Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002946

Physical Location & Seq

Genomic Coordinates Forward (+)

36307986 .. 36310742

Gene Structure 2757 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1229700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGAGTCGCCGTTCAAGGCTGATGCGGTCAAAGGGAAGGTGACTCTTATAACCGGAGGCGGGTCAGGAATCGGGTTCGAAATATCGACCCAATTCGGCAAGCATGGAGCATCGATTGCCATCATGGGCCGCCGGAAGCAAGTGCTGGACTCTGCCGTCTCCGAGCTTCGCTCTCTTGGAATTCAGGCTGTTGGTTTTGTGGGGGATGTTCGTAAACAGGAAGATGCAAAACGAGTGGTGGAATCAACTTTCCAGCATTTTGGTAGGCTTGACATACTTGTGAATGCCGCAGCTGGCAATTTTCTTGCATCAGCAGAGGACCTGTCCCCTAATGGATTTCGAACAGTTATGGATATTGATGCTGTTGGCACATTCACAATGTGCTACGAAGTACTCAAGTATCTTAAAAAAGGAGGTCCTGGAAGGGGCTCATCTGGTGGTGGAACAATATTGAACATCAGTGCTACTTTACATTATACGGCTGCTTGGTATCAAGTCCATGTATCAGCCGCCAAGGCAGCTGTTGATGCCACTATGAGAAACTTGGCACTGGAGTGGGGAACTGACTACGACATTAGGGTCAATGGAATAGCACCGGGTCCCATTGGTGATACGCCTGGCATGAGTAAACTTGCACCAACAGAGATAAATAGCAAAGCTAGAGATTACATGCCTTTGTATAAACTAGGGGACAAATGGGATATTGCTATGGCCGCTCTCTACCTTACTTCAGATGCAGCTTTTCAGTGTCATCCGGTCCTATTATCCTTTGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0016020 GO:0031090 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04146 map04146

Pfam Domains

Protein Families

adh_short_C2

Protein Analysis

259

Amino Acids

27.51

Weight (kDa)

6.97

Isoelectric Point (pI)

32.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	13 - 212	5.6e-40	short chain dehydrogenase
SDR	PF23441	13 - 202	1e-05	SDR-like rossmann domain
KR	PF08659	15 - 118	1.1e-07	KR domain
adh_short_C2	PF13561	22 - 247	2.4e-41	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002946)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12800
fragaria_vesca	FvH4_3g12490 FvH4_3g12490 FvH4_3g14740 FvH4_3g14740
malus_domestica	MD05G1229700.v1.1 MD05G1249900.v1.1 MD10G1209100.v1.1
prunus_persica	Prupe.4G113900_v2.0.a1 Prupe.4G132600_v2.0.a1
pyrus_communis	pycom05g22760 pycom10g17790
rosa_chinensis	RchiOBHm_Chr5g0020441 RchiOBHm_Chr5g0024271
rosa_laevigata	RLG00000032530 RLG00000032789
rosa_multiflora	Rmu_sc0000033.1_g000008 Rmu_sc0000033.1_g000009 Rmu_sc0002344.1_g000003
rosa_roxburghii	Rroxscaffold_1G00054910 Rroxscaffold_1G00055770 Rroxscaffold_1G00057840
rosa_rugosa	Rorug05G0059300 Rorug05G0082900
rosa_samantha	Rh5AG150100 Rh5AG150300 Rh5AG173400 Rh5BG149300 Rh5BG170300 Rh5CG160900 Rh5CG186400 Rh5DG148500 Rh5DG148700 Rh5DG172900
rosa_wichuraiana	Rw0G000860 Rw5G013280 Rw5G013290 Rw5G015660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	50
AasI	GACNNNNNNGTC	2 cut(s)	155, 578
AccBSI	CCGCTC	1 cut(s)	716
AciI	CCGC	6 cut(s)	26, 60, 130, 288, 510, 714
AcoI	YGGCCR	1 cut(s)	711
AcsI	RAATTY	1 cut(s)	180
AcuI	CTGAAG	1 cut(s)	714
AdeI	CACNNNGTG	1 cut(s)	381
AfaI	GTAC	1 cut(s)	393
AfiI	CCNNNNNNNGG	2 cut(s)	60, 425
AgsI	TTSAA	2 cut(s)	16, 454
AjnI	CCWGG	2 cut(s)	418, 616
AleI	CACNNNNGTG	1 cut(s)	553
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	5 cut(s)	166, 293, 521, 659, 740
AluI	AGCT	5 cut(s)	166, 293, 521, 659, 740
Alw26I	GTCTC	1 cut(s)	163
AoxI	GGCC	2 cut(s)	127, 711
ApeKI	GCWGC	4 cut(s)	290, 482, 518, 737
ApoI	RAATTY	1 cut(s)	180
AspS9I	GGNCC	5 cut(s)	127, 319, 416, 599, 757
AsuC2I	CCSGG	1 cut(s)	597
AsuHPI	GGTGA	2 cut(s)	52, 620
AsuII	TTCGAA	2 cut(s)	78, 340
AvaII	GGWCC	4 cut(s)	319, 416, 599, 757
BaeI	ACNNNNGTAYC	2 cut(s)	383, 416
BanII	GRGCYC	1 cut(s)	431
BbvI	GCAGC	4 cut(s)	302, 469, 530, 749
BccI	CCATC	1 cut(s)	128
BceAI	ACGGC	2 cut(s)	140, 495
BciT130I	CCWGG	2 cut(s)	420, 618
BcnI	CCSGG	1 cut(s)	597
BcoDI	GTCTC	1 cut(s)	163
BfaI	CTAG	2 cut(s)	660, 686
BglI	GCCNNNNNGGC	1 cut(s)	515
BisI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 738
BlsI	GCNGC	8 cut(s)	131, 289, 292, 484, 511, 520, 715, 739
BmcAI	AGTACT	1 cut(s)	393
Bme1390I	CCNGG	3 cut(s)	420, 597, 618
Bme18I	GGWCC	4 cut(s)	319, 416, 599, 757
BmgT120I	GGNCC	5 cut(s)	127, 319, 416, 599, 757
BmiI	GGNNCC	2 cut(s)	600, 601
BmrFI	CCNGG	3 cut(s)	420, 597, 618
BmsI	GCATC	7 cut(s)	13, 119, 214, 317, 349, 517, 724
BplI	GAGNNNNNCTC	2 cut(s)	155, 187
BpmI	CTGGAG	1 cut(s)	572
Bpu14I	TTCGAA	2 cut(s)	78, 340
BpuEI	CTTGAG	1 cut(s)	380
BpuMI	CCSGG	1 cut(s)	597
Bsa29I	ATCGAT	1 cut(s)	113
BsaBI	GATNNNNATC	1 cut(s)	119
BsaJI	CCNNGG	1 cut(s)	513
BsaWI	WCCGGW	2 cut(s)	53, 754
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	2 cut(s)	60, 425
Bse1I	ACTGG	1 cut(s)	555
Bse8I	GATNNNNATC	1 cut(s)	119
BseBI	CCWGG	2 cut(s)	420, 618
BseCI	ATCGAT	1 cut(s)	113
BseDI	CCNNGG	1 cut(s)	513
BseGI	GGATG	2 cut(s)	211, 751
BseJI	GATNNNNATC	1 cut(s)	119
BseLI	CCNNNNNNNGG	2 cut(s)	60, 425
BseNI	ACTGG	1 cut(s)	555
BseXI	GCAGC	4 cut(s)	302, 469, 530, 749
BshFI	GGCC	2 cut(s)	129, 713
BshVI	ATCGAT	1 cut(s)	113
BsiSI	CCGG	4 cut(s)	54, 133, 596, 755
BslFI	GGGAC	3 cut(s)	310, 585, 704
BslI	CCNNNNNNNGG	2 cut(s)	60, 425
BsmAI	GTCTC	1 cut(s)	163
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	3 cut(s)	310, 585, 704
BsmI	GAATGC	1 cut(s)	289
BsnI	GGCC	2 cut(s)	129, 713
Bsp119I	TTCGAA	2 cut(s)	78, 340
Bsp1286I	GDGCHC	1 cut(s)	431
BspACI	CCGC	6 cut(s)	26, 60, 130, 288, 510, 714
BspANI	GGCC	2 cut(s)	129, 713
BspDI	ATCGAT	1 cut(s)	113
BspLI	GGNNCC	2 cut(s)	600, 601
BspT104I	TTCGAA	2 cut(s)	78, 340
BsrBI	CCGCTC	1 cut(s)	716
BsrI	ACTGG	1 cut(s)	555
BssECI	CCNNGG	1 cut(s)	513
BssT1I	CCWWGG	1 cut(s)	513
Bst2UI	CCWGG	2 cut(s)	420, 618
Bst4CI	ACNGT	1 cut(s)	346
BstAPI	GCANNNNNTGC	1 cut(s)	116
BstBI	TTCGAA	2 cut(s)	78, 340
BstC8I	GCNNGC	2 cut(s)	101, 295
BstF5I	GGATG	2 cut(s)	211, 751
BstMAI	GTCTC	1 cut(s)	163
BstMWI	GCNNNNNNNGC	5 cut(s)	116, 515, 518, 527, 713
BstNI	CCWGG	2 cut(s)	420, 618
BstNSI	RCATGY	1 cut(s)	673
BstSCI	CCNGG	3 cut(s)	418, 595, 616
BstV1I	GCAGC	4 cut(s)	302, 469, 530, 749
BstXI	CCANNNNNNTGG	1 cut(s)	260
Bsu15I	ATCGAT	1 cut(s)	113
BsuRI	GGCC	2 cut(s)	129, 713
BsuTUI	ATCGAT	1 cut(s)	113
BtsCI	GGATG	2 cut(s)	211, 751
BtsIMutI	CAGTG	3 cut(s)	466, 548, 752
Cac8I	GCNNGC	2 cut(s)	101, 295
Cfr13I	GGNCC	5 cut(s)	127, 319, 416, 599, 757
ClaI	ATCGAT	1 cut(s)	113
Csp6I	GTAC	1 cut(s)	392
CspCI	CAANNNNNGTGG	2 cut(s)	216, 251
CviAII	CATG	5 cut(s)	104, 124, 500, 622, 670
CviQI	GTAC	1 cut(s)	392
DraIII	CACNNNGTG	1 cut(s)	381
DrdI	GACNNNNNNGTC	2 cut(s)	155, 578
DseDI	GACNNNNNNGTC	2 cut(s)	155, 578
EaeI	YGGCCR	1 cut(s)	711
Eco130I	CCWWGG	1 cut(s)	513
Eco24I	GRGCYC	1 cut(s)	431
Eco47I	GGWCC	4 cut(s)	319, 416, 599, 757
Eco57I	CTGAAG	1 cut(s)	714
EcoO109I	RGGNCCY	3 cut(s)	319, 416, 599
EcoRI	GAATTC	1 cut(s)	180
EcoRII	CCWGG	2 cut(s)	418, 616
EcoT14I	CCWWGG	1 cut(s)	513
EcoT38I	GRGCYC	1 cut(s)	431
ErhI	CCWWGG	1 cut(s)	513
Esp3I	CGTCTC	1 cut(s)	163
FaeI	CATG	5 cut(s)	107, 127, 503, 625, 673
FaqI	GGGAC	3 cut(s)	310, 585, 704
FatI	CATG	5 cut(s)	103, 123, 499, 621, 669
FauI	CCCGC	1 cut(s)	53
Fnu4HI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 738
FokI	GGATG	2 cut(s)	218, 738
FriOI	GRGCYC	1 cut(s)	431
Fsp4HI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 738
FspBI	CTAG	2 cut(s)	660, 686
GluI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 738
GsuI	CTGGAG	1 cut(s)	572
HaeIII	GGCC	2 cut(s)	129, 713
HapII	CCGG	4 cut(s)	54, 133, 596, 755
Hin1II	CATG	5 cut(s)	107, 127, 503, 625, 673
HinfI	GANTC	5 cut(s)	5, 43, 69, 149, 242
HpaII	CCGG	4 cut(s)	54, 133, 596, 755
HphI	GGTGA	2 cut(s)	52, 620
Hpy166II	GTNNAC	2 cut(s)	215, 629
Hpy188I	TCNGA	2 cut(s)	163, 733
Hpy188III	TCNNGA	1 cut(s)	66
Hpy8I	GTNNAC	2 cut(s)	215, 629
HpyAV	CCTTC	2 cut(s)	31, 417
HpyCH4III	ACNGT	1 cut(s)	346
HpyCH4V	TGCA	4 cut(s)	227, 308, 635, 737
HpyF10VI	GCNNNNNNNGC	5 cut(s)	116, 515, 518, 527, 713
Hsp92II	CATG	5 cut(s)	107, 127, 503, 625, 673
KflI	GGGWCCC	1 cut(s)	599
LmnI	GCTCC	1 cut(s)	107
Lsp1109I	GCAGC	4 cut(s)	302, 469, 530, 749
LweI	GCATC	7 cut(s)	13, 119, 214, 317, 349, 517, 724
MaeI	CTAG	2 cut(s)	660, 686
MaeIII	GTNAC	1 cut(s)	40
MbiI	CCGCTC	1 cut(s)	716
MboII	GAAGA	1 cut(s)	233
MhlI	GDGCHC	1 cut(s)	431
MluCI	AATT	3 cut(s)	92, 180, 298
MlyI	GAGTC	3 cut(s)	14, 37, 143
MnlI	CCTC	3 cut(s)	50, 310, 407
MseI	TTAA	1 cut(s)	405
MslI	CAYNNNNRTG	2 cut(s)	278, 553
MspA1I	CMGCKG	2 cut(s)	293, 521
MspI	CCGG	4 cut(s)	54, 133, 596, 755
MspR9I	CCNGG	3 cut(s)	420, 597, 618
Mva1269I	GAATGC	1 cut(s)	289
MvaI	CCWGG	2 cut(s)	420, 618
MwoI	GCNNNNNNNGC	5 cut(s)	116, 515, 518, 527, 713
NciI	CCSGG	1 cut(s)	597
NlaIII	CATG	5 cut(s)	107, 127, 503, 625, 673
NlaIV	GGNNCC	2 cut(s)	600, 601
NmuCI	GTSAC	1 cut(s)	40
NspI	RCATGY	1 cut(s)	673
NspV	TTCGAA	2 cut(s)	78, 340
OliI	CACNNNNGTG	1 cut(s)	553
PctI	GAATGC	1 cut(s)	289
PfeI	GAWTC	2 cut(s)	69, 242
PfoI	TCCNGGA	1 cut(s)	418
PkrI	GCNGC	8 cut(s)	131, 289, 292, 484, 511, 520, 715, 739
PleI	GAGTC	3 cut(s)	13, 37, 143
PpsI	GAGTC	3 cut(s)	13, 37, 143
PpuMI	RGGWCCY	3 cut(s)	319, 416, 599
PsiI	TTATAA	1 cut(s)	50
Psp5II	RGGWCCY	3 cut(s)	319, 416, 599
Psp6I	CCWGG	2 cut(s)	418, 616
PspGI	CCWGG	2 cut(s)	418, 616
PspN4I	GGNNCC	2 cut(s)	600, 601
PspPI	GGNCC	5 cut(s)	127, 319, 416, 599, 757
PspPPI	RGGWCCY	3 cut(s)	319, 416, 599
PvuII	CAGCTG	2 cut(s)	293, 521
RsaI	GTAC	1 cut(s)	393
RsaNI	GTAC	1 cut(s)	392
RseI	CAYNNNNRTG	2 cut(s)	278, 553
SaqAI	TTAA	1 cut(s)	405
SatI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 738
Sau96I	GGNCC	5 cut(s)	127, 319, 416, 599, 757
ScaI	AGTACT	1 cut(s)	393
SchI	GAGTC	3 cut(s)	14, 37, 143
ScrFI	CCNGG	3 cut(s)	420, 597, 618
SduI	GDGCHC	1 cut(s)	431
SetI	ASST	9 cut(s)	42, 168, 295, 324, 418, 523, 661, 726, 742
SfaNI	GCATC	7 cut(s)	13, 119, 214, 317, 349, 517, 724
SfuI	TTCGAA	2 cut(s)	78, 340
SinI	GGWCC	4 cut(s)	319, 416, 599, 757
SmiMI	CAYNNNNRTG	2 cut(s)	278, 553
SmlI	CTYRAG	1 cut(s)	395
SmoI	CTYRAG	1 cut(s)	395
Sse9I	AATT	3 cut(s)	92, 180, 298
SsiI	CCGC	6 cut(s)	26, 60, 130, 288, 510, 714
SspI	AATATT	1 cut(s)	450
SspMI	CTAG	2 cut(s)	660, 686
StyD4I	CCNGG	3 cut(s)	418, 595, 616
StyI	CCWWGG	1 cut(s)	513
TaaI	ACNGT	1 cut(s)	346
TaqI	TCGA	4 cut(s)	78, 86, 113, 340
TasI	AATT	3 cut(s)	92, 180, 298
TatI	WGTACW	1 cut(s)	391
TauI	GCSGC	4 cut(s)	132, 290, 512, 716
TfiI	GAWTC	2 cut(s)	69, 242
Tru1I	TTAA	1 cut(s)	405
Tru9I	TTAA	1 cut(s)	405
TscAI	CASTG	3 cut(s)	466, 555, 752
TseFI	GTSAC	1 cut(s)	40
TseI	GCWGC	4 cut(s)	290, 482, 518, 737
Tsp45I	GTSAC	1 cut(s)	40
TspRI	CASTG	3 cut(s)	466, 555, 752
VpaK11BI	GGWCC	4 cut(s)	319, 416, 599, 757
XapI	RAATTY	1 cut(s)	180
XceI	RCATGY	1 cut(s)	673
XspI	CTAG	2 cut(s)	660, 686
ZrmI	AGTACT	1 cut(s)	393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.