Rroxscaffold_1G00057840

Peroxisomal 2,4-dienoyl-CoA reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002946
Physical Location & Seq
Forward (+)
79844647 .. 79847770
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057840.1

Sequence Viewer

Length: 945 bp
ATGGAATCACCATTCAAGGCGGAGATACTGAAGGGCAAGGTGGCTTTGTTGACCGGAGGAGCCTCGGGAATTGGGTACGAGATATCAGTTCAGTTGGGCAAACATGGAGCCTCCATTGCCGTCATGGGTCGTCGTAAAAACGTCATCGACTCTGCTGTTGAATCCCTTCACTCCCTTGGCATTCCCGCTATTGGACTTGTGGGTGATGTTCGCAAAAGAGATGATGCAGTTAGAGTTTTGGAATCAACTGTCAAGCATTTTGGTAGGCTCGATATCCTTGTGAATGCTGCTGCTGGCAATTTCCTTGTCCCGTCTGAGGATCTATCCCCAAATGGATTTCGAACAGTTTTAGATATAGATGCTGTTGGCACCTTTACGATGTGTCATGAAGCACTCAAGTATCTCAAGAAGGGGGCACCAGGGAAGGACTCATCTGTTGGTGGAACAATAATAAACATAAGTGTGACTTTGCATTATACAGCTACTTGGTATCAAATTCATGTATCTGCAGCAAAGGCTGAAAAGGCTGATGTGACTGAAATGGGTTCGTTCTTTCTTCTATACCAGGCTGCTGTTGATAGCATTACAAGAAGCTTGGCATTGGAATGGGGAACAGATTATGATATCAGAGTCAATGGTATTGCGCCAGGACCTATTGAAGACACAGCTGGCTTTAGTAAACTCTTACTGCAGGAGATTTTGAGCCAATTTAATGTGAATGCTCCTACAACAAAAGTAGGACAGAAATGGGATATTGCTATGGCTGCAGTATATCTTGCGTCTACCGCAGGGAAATACATCAATGGAACAACATTGGTTGTAGATGGGGCAGATTGGTTGACCAAACCATCCCATCTTCCGAAAGAGACGGTAAAGCAACTGTCCCGAGCTGTGGAGCGAAGGTCTAGAAATGAACCAGTTGGCATTCCAAAGAGCAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

314

Amino Acids

33.65

Weight (kDa)

8.77

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 173 4.2e-28 short chain dehydrogenase
KR PF08659 15 - 122 1.6e-06 KR domain
adh_short_C2 PF13561 21 - 173 1.2e-20 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 189 - 276 6.7e-18 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 368, 415
AccI GTMKAC 1 cut(s) 782
AciI CCGC 3 cut(s) 20, 186, 786
AclWI GGATC 1 cut(s) 327
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 3 cut(s) 191, 316, 892
AgsI TTSAA 3 cut(s) 16, 161, 659
AjnI CCWGG 3 cut(s) 418, 564, 646
AluBI AGCT 5 cut(s) 482, 594, 668, 890, 940
AluI AGCT 5 cut(s) 482, 594, 668, 890, 940
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 1 cut(s) 327
Ama87I CYCGRG 2 cut(s) 64, 885
ApeKI GCWGC 5 cut(s) 287, 290, 509, 569, 764
ApoI RAATTY 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 165
AspLEI GCGC 1 cut(s) 646
AspS9I GGNCC 1 cut(s) 650
AsuHPI GGTGA 1 cut(s) 215
AsuII TTCGAA 1 cut(s) 340
AvaI CYCGRG 2 cut(s) 64, 885
AvaII GGWCC 1 cut(s) 650
BaeGI GKGCMC 1 cut(s) 418
BaeI ACNNNNGTAYC 2 cut(s) 383, 416
BanI GGYRCC 2 cut(s) 368, 415
BbsI GAAGAC 1 cut(s) 666
BbvI GCAGC 5 cut(s) 274, 277, 521, 556, 751
BccI CCATC 3 cut(s) 818, 856, 861
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 3 cut(s) 420, 566, 648
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 1 cut(s) 906
BfmI CTRYAG 3 cut(s) 507, 689, 765
BisI GCNGC 5 cut(s) 288, 291, 510, 570, 765
BlsI GCNGC 5 cut(s) 289, 292, 511, 571, 766
Bme1390I CCNGG 3 cut(s) 420, 566, 648
Bme18I GGWCC 1 cut(s) 650
BmeT110I CYCGRG 2 cut(s) 64, 885
BmgT120I GGNCC 1 cut(s) 650
BmiI GGNNCC 4 cut(s) 61, 109, 370, 417
BmrFI CCNGG 3 cut(s) 420, 566, 648
BmsI GCATC 2 cut(s) 214, 349
BpiI GAAGAC 1 cut(s) 666
Bpu14I TTCGAA 1 cut(s) 340
BpuEI CTTGAG 2 cut(s) 380, 389
BsaJI CCNNGG 3 cut(s) 63, 175, 419
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 887, 917
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 316, 892
Bse1I ACTGG 1 cut(s) 917
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 3 cut(s) 420, 566, 648
BseDI CCNNGG 3 cut(s) 63, 175, 419
BseGI GGATG 1 cut(s) 848
BseLI CCNNNNNNNGG 3 cut(s) 191, 316, 892
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 1 cut(s) 917
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 5 cut(s) 274, 277, 521, 556, 751
BshNI GGYRCC 2 cut(s) 368, 415
BsiHKCI CYCGRG 2 cut(s) 64, 885
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 2 cut(s) 293, 868
BslI CCNNNNNNNGG 3 cut(s) 191, 316, 892
BsmAI GTCTC 1 cut(s) 860
BsmBI CGTCTC 1 cut(s) 860
BsmFI GGGAC 2 cut(s) 293, 868
BsmI GAATGC 4 cut(s) 180, 289, 724, 924
BsoBI CYCGRG 2 cut(s) 64, 885
Bsp119I TTCGAA 1 cut(s) 340
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 1 cut(s) 319
BspACI CCGC 3 cut(s) 20, 186, 786
BspCNI CTCAG 1 cut(s) 307
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 4 cut(s) 61, 109, 370, 417
BspMAI CTGCAG 3 cut(s) 511, 693, 769
BspPI GGATC 1 cut(s) 327
BspT104I TTCGAA 1 cut(s) 340
BspT107I GGYRCC 2 cut(s) 368, 415
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 1 cut(s) 917
BssECI CCNNGG 3 cut(s) 63, 175, 419
BssMI GATC 1 cut(s) 319
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 3 cut(s) 420, 566, 648
Bst4CI ACNGT 4 cut(s) 250, 346, 871, 882
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 3 cut(s) 295, 670, 938
BstDEI CTNAG 1 cut(s) 315
BstF5I GGATG 1 cut(s) 848
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 1 cut(s) 322
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 1 cut(s) 319
BstMWI GCNNNNNNNGC 5 cut(s) 116, 515, 524, 764, 785
BstNI CCWGG 3 cut(s) 420, 566, 648
BstSCI CCNGG 3 cut(s) 418, 564, 646
BstSFI CTRYAG 3 cut(s) 507, 689, 765
BstSLI GKGCMC 1 cut(s) 418
BstV1I GCAGC 5 cut(s) 274, 277, 521, 556, 751
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BtsCI GGATG 1 cut(s) 848
Cac8I GCNNGC 3 cut(s) 295, 670, 938
CciI TCATGA 1 cut(s) 385
CfoI GCGC 1 cut(s) 646
Cfr13I GGNCC 1 cut(s) 650
CseI GACGC 1 cut(s) 768
Csp6I GTAC 1 cut(s) 76
CviAII CATG 4 cut(s) 104, 124, 386, 500
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 1 cut(s) 315
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
EciI GGCGGA 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 175
Eco32I GATATC 3 cut(s) 84, 274, 625
Eco47I GGWCC 1 cut(s) 650
Eco57I CTGAAG 1 cut(s) 50
Eco88I CYCGRG 2 cut(s) 64, 885
EcoO109I RGGNCCY 1 cut(s) 650
EcoRII CCWGG 3 cut(s) 418, 564, 646
EcoRV GATATC 3 cut(s) 84, 274, 625
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 860
FaeI CATG 4 cut(s) 107, 127, 389, 503
FalI AAGNNNNNCTT 2 cut(s) 451, 483
FaqI GGGAC 2 cut(s) 293, 868
FatI CATG 4 cut(s) 103, 123, 385, 499
FauI CCCGC 1 cut(s) 193
FblI GTMKAC 1 cut(s) 782
Fnu4HI GCNGC 5 cut(s) 288, 291, 510, 570, 765
FokI GGATG 1 cut(s) 835
Fsp4HI GCNGC 5 cut(s) 288, 291, 510, 570, 765
FspBI CTAG 1 cut(s) 906
GlaI GCGC 1 cut(s) 645
GluI GCNGC 5 cut(s) 288, 291, 510, 570, 765
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 768
HhaI GCGC 1 cut(s) 646
Hin1II CATG 4 cut(s) 107, 127, 389, 503
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HincII GTYRAC 2 cut(s) 51, 840
HindII GTYRAC 2 cut(s) 51, 840
HindIII AAGCTT 1 cut(s) 592
HinfI GANTC 6 cut(s) 5, 149, 161, 242, 428, 630
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 4 cut(s) 51, 680, 783, 840
Hpy188I TCNGA 3 cut(s) 316, 629, 861
Hpy188III TCNNGA 5 cut(s) 66, 386, 406, 885, 906
Hpy8I GTNNAC 4 cut(s) 51, 680, 783, 840
Hpy99I CGWCG 1 cut(s) 135
HpyAV CCTTC 5 cut(s) 25, 176, 403, 418, 894
HpyCH4III ACNGT 4 cut(s) 250, 346, 871, 882
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 5 cut(s) 227, 472, 509, 691, 767
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 515, 524, 764, 785
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 4 cut(s) 107, 127, 389, 503
HspAI GCGC 1 cut(s) 644
Kzo9I GATC 1 cut(s) 319
LmnI GCTCC 4 cut(s) 59, 107, 727, 895
Lsp1109I GCAGC 5 cut(s) 274, 277, 521, 556, 751
LweI GCATC 2 cut(s) 214, 349
MaeI CTAG 1 cut(s) 906
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 2 cut(s) 463, 532
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 3 cut(s) 548, 671, 848
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 4 cut(s) 69, 298, 495, 707
MlyI GAGTC 3 cut(s) 143, 422, 639
MnlI CCTC 4 cut(s) 50, 73, 121, 310
MroXI GAANNNNTTC 1 cut(s) 165
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 2 cut(s) 461, 604
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 3 cut(s) 420, 566, 648
Mva1269I GAATGC 4 cut(s) 180, 289, 724, 924
MvaI CCWGG 3 cut(s) 420, 566, 648
MwoI GCNNNNNNNGC 5 cut(s) 116, 515, 524, 764, 785
NdeII GATC 1 cut(s) 319
NlaIII CATG 4 cut(s) 107, 127, 389, 503
NlaIV GGNNCC 4 cut(s) 61, 109, 370, 417
NmuCI GTSAC 2 cut(s) 463, 532
NspV TTCGAA 1 cut(s) 340
PagI TCATGA 1 cut(s) 385
PctI GAATGC 4 cut(s) 180, 289, 724, 924
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 3 cut(s) 5, 161, 242
PkrI GCNGC 5 cut(s) 289, 292, 511, 571, 766
PleI GAGTC 3 cut(s) 143, 422, 638
PpsI GAGTC 3 cut(s) 143, 422, 638
PpuMI RGGWCCY 1 cut(s) 650
Psp5II RGGWCCY 1 cut(s) 650
Psp6I CCWGG 3 cut(s) 418, 564, 646
PspGI CCWGG 3 cut(s) 418, 564, 646
PspN4I GGNNCC 4 cut(s) 61, 109, 370, 417
PspPI GGNCC 1 cut(s) 650
PspPPI RGGWCCY 1 cut(s) 650
PstI CTGCAG 3 cut(s) 511, 693, 769
PsuI RGATCY 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
RseI CAYNNNNRTG 2 cut(s) 461, 604
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 5 cut(s) 288, 291, 510, 570, 765
Sau3AI GATC 1 cut(s) 319
Sau96I GGNCC 1 cut(s) 650
SchI GAGTC 3 cut(s) 143, 422, 639
ScrFI CCNGG 3 cut(s) 420, 566, 648
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 2 cut(s) 214, 349
SfcI CTRYAG 3 cut(s) 507, 689, 765
SfuI TTCGAA 1 cut(s) 340
SinI GGWCC 1 cut(s) 650
SmiMI CAYNNNNRTG 2 cut(s) 461, 604
SmlI CTYRAG 2 cut(s) 395, 404
SmoI CTYRAG 2 cut(s) 395, 404
Sse9I AATT 4 cut(s) 69, 298, 495, 707
SsiI CCGC 3 cut(s) 20, 186, 786
SspMI CTAG 1 cut(s) 906
StyD4I CCNGG 3 cut(s) 418, 564, 646
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 4 cut(s) 250, 346, 871, 882
TaiI ACGT 1 cut(s) 144
TaqI TCGA 3 cut(s) 147, 270, 340
TasI AATT 4 cut(s) 69, 298, 495, 707
TfiI GAWTC 3 cut(s) 5, 161, 242
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TseFI GTSAC 2 cut(s) 463, 532
TseI GCWGC 5 cut(s) 287, 290, 509, 569, 764
Tsp45I GTSAC 2 cut(s) 463, 532
TspDTI ATGAA 3 cut(s) 402, 488, 927
VpaK11BI GGWCC 1 cut(s) 650
XapI RAATTY 1 cut(s) 495
XbaI TCTAGA 1 cut(s) 905
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 165
XspI CTAG 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.