Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0002946

Physical Location & Seq

Genomic Coordinates Forward (+)

20961514 .. 20964430

Gene Structure 2917 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g17790.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 894 bp

ATGGAGTCGCCGTTCAAGGCTGATGCGGTGAAAGGGAAGGTGGCTCTGATAACCGGAGGTGGGTCAGGAATCGGGTTGGAAATGTCGACCCAATTCGGCAAACATGGCGCCTCCATTGCCATCATGGGCCGCCGGAAGCAAGTGCTTGACTCAGCCGTCTCCGAGCTTCGATCTCTTGGAATTCAGGCTGTTGGTTTTGAGGGAGATGTTCGTAAACAGGAAGATGCAAAAAGAGTGGTGGAATCAACTTTCCAGCATTTTGGTAGGCTTGACGTACTTGTGAATGCTGCAGCTGGCAATTTTCTTGTATCAGCAGAGGATCTATCACCTAATGGATTTCGAACAGTTATGGATATTGATGCTGTTGGTACATTCACAATGTGCCACGAAGCACTCAAGTATCTTAAAAAAGGAGGGCCCGGAAGGGGCTCATCTGGTGGTGGAACAATATTGAACATCAGTGTTACTTTACATTATACAGCCGCTTGGTATCAAGTCCATGTATCAGCAGCCAAGGCAGCTGTTGATGCCACTATGAGAAACTTGGCACTCGAGTGGGGAACTGACTACGACATTAGGGTCAATGGAATAGCACCGGGTCCCATTGGTGATACACCTGGCATGAGTAAACTTGCACCTGCGGAGATAAATAGCAAAGCTAGAGATTACATGCCTTTGTATAAACTAGGGGACAAATGGGATATTGCTATGGCCGCTCTCTACCTTACTTCGGATGCAGGTAAATTCATCAATGGAACTGTCATTGTAGTTGATGGGGGACTTTGGCTGAGCCGGCCTCGCCATCTGCCAAAAGAAGCAGTGAAGCAGCTTTCTCGAACAGTAGAGAACAGATCTAGAAATGCACCAGTAGGGGTTCCAAACAGCAAGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0016020 GO:0031090 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04146 map04146

Pfam Domains

Protein Families

adh_short_C2

Protein Analysis

298

Amino Acids

31.66

Weight (kDa)

9.3

Isoelectric Point (pI)

37.52

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002946)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12800
fragaria_vesca	FvH4_3g12490 FvH4_3g12490 FvH4_3g14740 FvH4_3g14740
malus_domestica	MD05G1229700.v1.1 MD05G1249900.v1.1 MD10G1209100.v1.1
prunus_persica	Prupe.4G113900_v2.0.a1 Prupe.4G132600_v2.0.a1
pyrus_communis	pycom05g22760 pycom10g17790
rosa_chinensis	RchiOBHm_Chr5g0020441 RchiOBHm_Chr5g0024271
rosa_laevigata	RLG00000032530 RLG00000032789
rosa_multiflora	Rmu_sc0000033.1_g000008 Rmu_sc0000033.1_g000009 Rmu_sc0002344.1_g000003
rosa_roxburghii	Rroxscaffold_1G00054910 Rroxscaffold_1G00055770 Rroxscaffold_1G00057840
rosa_rugosa	Rorug05G0059300 Rorug05G0082900
rosa_samantha	Rh5AG150100 Rh5AG150300 Rh5AG173400 Rh5BG149300 Rh5BG170300 Rh5CG160900 Rh5CG186400 Rh5DG148500 Rh5DG148700 Rh5DG172900
rosa_wichuraiana	Rw0G000860 Rw5G013280 Rw5G013290 Rw5G015660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	646
AasI	GACNNNNNNGTC	2 cut(s)	155, 578
Acc36I	ACCTGC	2 cut(s)	646, 728
AccB1I	GGYRCC	1 cut(s)	107
AccBSI	CCGCTC	1 cut(s)	716
AccI	GTMKAC	1 cut(s)	86
AciI	CCGC	5 cut(s)	26, 130, 483, 641, 714
AclWI	GGATC	1 cut(s)	327
AcoI	YGGCCR	1 cut(s)	711
AcsI	RAATTY	2 cut(s)	180, 743
AcyI	GRCGYC	1 cut(s)	108
AdeI	CACNNNGTG	1 cut(s)	381
AfaI	GTAC	2 cut(s)	276, 370
AfiI	CCNNNNNNNGG	3 cut(s)	60, 425, 730
AgsI	TTSAA	2 cut(s)	16, 454
AjnI	CCWGG	1 cut(s)	616
AleI	CACNNNNGTG	1 cut(s)	553
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	5 cut(s)	166, 293, 521, 659, 829
AluI	AGCT	5 cut(s)	166, 293, 521, 659, 829
Alw26I	GTCTC	1 cut(s)	163
AlwI	GGATC	1 cut(s)	327
Ama87I	CYCGRG	1 cut(s)	551
AoxI	GGCC	4 cut(s)	127, 416, 711, 794
ApaI	GGGCCC	1 cut(s)	420
ApeKI	GCWGC	5 cut(s)	287, 290, 509, 518, 826
ApoI	RAATTY	2 cut(s)	180, 743
AspLEI	GCGC	1 cut(s)	110
AspS9I	GGNCC	4 cut(s)	127, 416, 417, 599
AsuC2I	CCSGG	2 cut(s)	420, 597
AsuHPI	GGTGA	3 cut(s)	40, 318, 620
AsuII	TTCGAA	1 cut(s)	340
AvaI	CYCGRG	1 cut(s)	551
AvaII	GGWCC	1 cut(s)	599
BaeGI	GKGCMC	1 cut(s)	420
BaeI	ACNNNNGTAYC	2 cut(s)	383, 416
BanI	GGYRCC	1 cut(s)	107
BanII	GRGCYC	2 cut(s)	420, 431
BbvI	GCAGC	5 cut(s)	274, 302, 521, 530, 838
BccI	CCATC	3 cut(s)	128, 767, 810
BceAI	ACGGC	1 cut(s)	140
BcgI	CGANNNNNNTGC	2 cut(s)	815, 849
BciT130I	CCWGG	1 cut(s)	618
BcnI	CCSGG	2 cut(s)	420, 597
BcoDI	GTCTC	1 cut(s)	163
BfaI	CTAG	3 cut(s)	660, 686, 855
BfmI	CTRYAG	1 cut(s)	288
BfoI	RGCGCY	1 cut(s)	111
BfuAI	ACCTGC	2 cut(s)	646, 728
BglII	AGATCT	1 cut(s)	851
BisI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 827
BlpI	GCTNAGC	1 cut(s)	788
BlsI	GCNGC	8 cut(s)	131, 289, 292, 484, 511, 520, 715, 828
Bme1390I	CCNGG	3 cut(s)	420, 597, 618
Bme18I	GGWCC	1 cut(s)	599
BmeT110I	CYCGRG	1 cut(s)	551
BmgT120I	GGNCC	4 cut(s)	127, 416, 417, 599
BmiI	GGNNCC	5 cut(s)	109, 418, 600, 601, 876
BmrFI	CCNGG	3 cut(s)	420, 597, 618
BmsI	GCATC	5 cut(s)	13, 214, 349, 517, 724
BplI	GAGNNNNNCTC	2 cut(s)	781, 813
Bpu1102I	GCTNAGC	1 cut(s)	788
Bpu14I	TTCGAA	1 cut(s)	340
BpuEI	CTTGAG	1 cut(s)	380
BpuMI	CCSGG	2 cut(s)	420, 597
BsaHI	GRCGYC	1 cut(s)	108
BsaJI	CCNNGG	1 cut(s)	513
BsaWI	WCCGGW	1 cut(s)	53
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	3 cut(s)	60, 425, 730
Bse118I	RCCGGY	1 cut(s)	792
Bse1I	ACTGG	1 cut(s)	866
Bse3DI	GCAATG	1 cut(s)	114
BseBI	CCWGG	1 cut(s)	618
BseDI	CCNNGG	1 cut(s)	513
BseGI	GGATG	1 cut(s)	739
BseLI	CCNNNNNNNGG	3 cut(s)	60, 425, 730
BseMI	GCAATG	1 cut(s)	114
BseMII	CTCAG	2 cut(s)	165, 779
BseNI	ACTGG	1 cut(s)	866
BseSI	GKGCMC	1 cut(s)	420
BseXI	GCAGC	5 cut(s)	274, 302, 521, 530, 838
BshFI	GGCC	4 cut(s)	129, 418, 713, 796
BshNI	GGYRCC	1 cut(s)	107
BsiHKCI	CYCGRG	1 cut(s)	551
BsiSI	CCGG	5 cut(s)	54, 133, 420, 596, 793
BslFI	GGGAC	3 cut(s)	585, 704, 792
BslI	CCNNNNNNNGG	3 cut(s)	60, 425, 730
BsmAI	GTCTC	1 cut(s)	163
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	3 cut(s)	585, 704, 792
BsmI	GAATGC	1 cut(s)	289
BsnI	GGCC	4 cut(s)	129, 418, 713, 796
BsoBI	CYCGRG	1 cut(s)	551
Bsp119I	TTCGAA	1 cut(s)	340
Bsp120I	GGGCCC	1 cut(s)	416
Bsp1286I	GDGCHC	2 cut(s)	420, 431
Bsp143I	GATC	3 cut(s)	170, 319, 851
Bsp1720I	GCTNAGC	1 cut(s)	788
BspACI	CCGC	5 cut(s)	26, 130, 483, 641, 714
BspANI	GGCC	4 cut(s)	129, 418, 713, 796
BspCNI	CTCAG	2 cut(s)	164, 780
BspLI	GGNNCC	5 cut(s)	109, 418, 600, 601, 876
BspMAI	CTGCAG	1 cut(s)	292
BspMI	ACCTGC	2 cut(s)	646, 728
BspPI	GGATC	1 cut(s)	327
BspT104I	TTCGAA	1 cut(s)	340
BspT107I	GGYRCC	1 cut(s)	107
BsrBI	CCGCTC	1 cut(s)	716
BsrDI	GCAATG	1 cut(s)	114
BsrFI	RCCGGY	1 cut(s)	792
BsrI	ACTGG	1 cut(s)	866
BssAI	RCCGGY	1 cut(s)	792
BssECI	CCNNGG	1 cut(s)	513
BssMI	GATC	3 cut(s)	170, 319, 851
BssNI	GRCGYC	1 cut(s)	108
BssT1I	CCWWGG	1 cut(s)	513
Bst2UI	CCWGG	1 cut(s)	618
Bst4CI	ACNGT	3 cut(s)	346, 760, 841
BstACI	GRCGYC	1 cut(s)	108
BstBI	TTCGAA	1 cut(s)	340
BstC8I	GCNNGC	2 cut(s)	295, 794
BstDEI	CTNAG	2 cut(s)	151, 788
BstF5I	GGATG	1 cut(s)	739
BstH2I	RGCGCY	1 cut(s)	111
BstHHI	GCGC	1 cut(s)	110
BstKTI	GATC	3 cut(s)	173, 322, 854
BstMAI	GTCTC	1 cut(s)	163
BstMBI	GATC	3 cut(s)	170, 319, 851
BstMWI	GCNNNNNNNGC	8 cut(s)	105, 116, 515, 518, 527, 713, 793, 798
BstNI	CCWGG	1 cut(s)	618
BstNSI	RCATGY	1 cut(s)	673
BstSCI	CCNGG	3 cut(s)	418, 595, 616
BstSFI	CTRYAG	1 cut(s)	288
BstSLI	GKGCMC	1 cut(s)	420
BstV1I	GCAGC	5 cut(s)	274, 302, 521, 530, 838
BstX2I	RGATCY	2 cut(s)	319, 851
BstXI	CCANNNNNNTGG	1 cut(s)	260
BstYI	RGATCY	2 cut(s)	319, 851
BsuRI	GGCC	4 cut(s)	129, 418, 713, 796
BtsCI	GGATG	1 cut(s)	739
BtsI	GCAGTG	1 cut(s)	825
BtsIMutI	CAGTG	2 cut(s)	466, 825
BveI	ACCTGC	2 cut(s)	646, 728
Cac8I	GCNNGC	2 cut(s)	295, 794
CfoI	GCGC	1 cut(s)	110
Cfr10I	RCCGGY	1 cut(s)	792
Cfr13I	GGNCC	4 cut(s)	127, 416, 417, 599
Csp6I	GTAC	2 cut(s)	275, 369
CspCI	CAANNNNNGTGG	2 cut(s)	216, 251
CviAII	CATG	5 cut(s)	104, 124, 500, 622, 670
CviQI	GTAC	2 cut(s)	275, 369
DdeI	CTNAG	2 cut(s)	151, 788
DinI	GGCGCC	1 cut(s)	109
DpnI	GATC	3 cut(s)	172, 321, 853
DpnII	GATC	3 cut(s)	170, 319, 851
DraIII	CACNNNGTG	1 cut(s)	381
DrdI	GACNNNNNNGTC	2 cut(s)	155, 578
DseDI	GACNNNNNNGTC	2 cut(s)	155, 578
EaeI	YGGCCR	1 cut(s)	711
Eco130I	CCWWGG	1 cut(s)	513
Eco24I	GRGCYC	2 cut(s)	420, 431
Eco47I	GGWCC	1 cut(s)	599
Eco88I	CYCGRG	1 cut(s)	551
EcoO109I	RGGNCCY	2 cut(s)	416, 599
EcoRI	GAATTC	1 cut(s)	180
EcoRII	CCWGG	1 cut(s)	616
EcoT14I	CCWWGG	1 cut(s)	513
EcoT38I	GRGCYC	2 cut(s)	420, 431
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	1 cut(s)	513
Esp3I	CGTCTC	1 cut(s)	163
FaeI	CATG	5 cut(s)	107, 127, 503, 625, 673
FaqI	GGGAC	3 cut(s)	585, 704, 792
FatI	CATG	5 cut(s)	103, 123, 499, 621, 669
FblI	GTMKAC	1 cut(s)	86
Fnu4HI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 827
FokI	GGATG	1 cut(s)	746
FriOI	GRGCYC	2 cut(s)	420, 431
Fsp4HI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 827
FspBI	CTAG	3 cut(s)	660, 686, 855
GlaI	GCGC	1 cut(s)	109
GluI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 827
HaeII	RGCGCY	1 cut(s)	111
HaeIII	GGCC	4 cut(s)	129, 418, 713, 796
HapII	CCGG	5 cut(s)	54, 133, 420, 596, 793
HhaI	GCGC	1 cut(s)	110
Hin1I	GRCGYC	1 cut(s)	108
Hin1II	CATG	5 cut(s)	107, 127, 503, 625, 673
Hin6I	GCGC	1 cut(s)	108
HinP1I	GCGC	1 cut(s)	108
HincII	GTYRAC	1 cut(s)	87
HindII	GTYRAC	1 cut(s)	87
HinfI	GANTC	4 cut(s)	5, 69, 149, 242
HpaII	CCGG	5 cut(s)	54, 133, 420, 596, 793
HphI	GGTGA	3 cut(s)	40, 318, 620
Hpy166II	GTNNAC	3 cut(s)	87, 215, 629
Hpy188I	TCNGA	3 cut(s)	48, 163, 733
Hpy188III	TCNNGA	3 cut(s)	66, 834, 855
Hpy8I	GTNNAC	3 cut(s)	87, 215, 629
HpyAV	CCTTC	2 cut(s)	31, 417
HpyCH4III	ACNGT	3 cut(s)	346, 760, 841
HpyCH4IV	ACGT	1 cut(s)	273
HpyCH4V	TGCA	5 cut(s)	227, 290, 635, 737, 863
HpyF10VI	GCNNNNNNNGC	8 cut(s)	105, 116, 515, 518, 527, 713, 793, 798
HpyF3I	CTNAG	2 cut(s)	151, 788
HpySE526I	ACGT	1 cut(s)	273
Hsp92I	GRCGYC	1 cut(s)	108
Hsp92II	CATG	5 cut(s)	107, 127, 503, 625, 673
HspAI	GCGC	1 cut(s)	108
KasI	GGCGCC	1 cut(s)	107
KflI	GGGWCCC	1 cut(s)	599
KroI	GCCGGC	1 cut(s)	792
KroNI	GCCGGC	1 cut(s)	794
Kzo9I	GATC	3 cut(s)	170, 319, 851
Lsp1109I	GCAGC	5 cut(s)	274, 302, 521, 530, 838
LweI	GCATC	5 cut(s)	13, 214, 349, 517, 724
MaeI	CTAG	3 cut(s)	660, 686, 855
MaeII	ACGT	1 cut(s)	273
MaeIII	GTNAC	1 cut(s)	463
MalI	GATC	3 cut(s)	172, 321, 853
MbiI	CCGCTC	1 cut(s)	716
MboI	GATC	3 cut(s)	170, 319, 851
MboII	GAAGA	1 cut(s)	233
MflI	RGATCY	2 cut(s)	319, 851
MhlI	GDGCHC	2 cut(s)	420, 431
MluCI	AATT	4 cut(s)	92, 180, 298, 743
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	2 cut(s)	14, 143
MmeI	TCCRAC	1 cut(s)	57
MnlI	CCTC	6 cut(s)	50, 121, 193, 310, 407, 807
MroNI	GCCGGC	1 cut(s)	792
MseI	TTAA	1 cut(s)	405
MslI	CAYNNNNRTG	1 cut(s)	553
MspA1I	CMGCKG	2 cut(s)	293, 521
MspI	CCGG	5 cut(s)	54, 133, 420, 596, 793
MspR9I	CCNGG	3 cut(s)	420, 597, 618
Mva1269I	GAATGC	1 cut(s)	289
MvaI	CCWGG	1 cut(s)	618
MwoI	GCNNNNNNNGC	8 cut(s)	105, 116, 515, 518, 527, 713, 793, 798
NaeI	GCCGGC	1 cut(s)	794
NarI	GGCGCC	1 cut(s)	108
NciI	CCSGG	2 cut(s)	420, 597
NdeII	GATC	3 cut(s)	170, 319, 851
NgoMIV	GCCGGC	1 cut(s)	792
NlaIII	CATG	5 cut(s)	107, 127, 503, 625, 673
NlaIV	GGNNCC	5 cut(s)	109, 418, 600, 601, 876
NspI	RCATGY	1 cut(s)	673
NspV	TTCGAA	1 cut(s)	340
OliI	CACNNNNGTG	1 cut(s)	553
PaeR7I	CTCGAG	1 cut(s)	551
PaqCI	CACCTGC	1 cut(s)	646
PctI	GAATGC	1 cut(s)	289
PdiI	GCCGGC	1 cut(s)	794
PfeI	GAWTC	2 cut(s)	69, 242
PkrI	GCNGC	8 cut(s)	131, 289, 292, 484, 511, 520, 715, 828
PleI	GAGTC	2 cut(s)	13, 143
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	2 cut(s)	13, 143
PpuMI	RGGWCCY	1 cut(s)	599
Psp5II	RGGWCCY	1 cut(s)	599
Psp6I	CCWGG	1 cut(s)	616
PspGI	CCWGG	1 cut(s)	616
PspN4I	GGNNCC	5 cut(s)	109, 418, 600, 601, 876
PspOMI	GGGCCC	1 cut(s)	416
PspPI	GGNCC	4 cut(s)	127, 416, 417, 599
PspPPI	RGGWCCY	1 cut(s)	599
PspXI	VCTCGAGB	1 cut(s)	551
PstI	CTGCAG	1 cut(s)	292
PsuI	RGATCY	2 cut(s)	319, 851
PvuII	CAGCTG	2 cut(s)	293, 521
RsaI	GTAC	2 cut(s)	276, 370
RsaNI	GTAC	2 cut(s)	275, 369
RseI	CAYNNNNRTG	1 cut(s)	553
SalI	GTCGAC	1 cut(s)	85
SaqAI	TTAA	1 cut(s)	405
SatI	GCNGC	8 cut(s)	130, 288, 291, 483, 510, 519, 714, 827
Sau3AI	GATC	3 cut(s)	170, 319, 851
Sau96I	GGNCC	4 cut(s)	127, 416, 417, 599
SchI	GAGTC	2 cut(s)	14, 143
ScrFI	CCNGG	3 cut(s)	420, 597, 618
SduI	GDGCHC	2 cut(s)	420, 431
SfaNI	GCATC	5 cut(s)	13, 214, 349, 517, 724
SfcI	CTRYAG	1 cut(s)	288
SfoI	GGCGCC	1 cut(s)	109
Sfr274I	CTCGAG	1 cut(s)	551
SfuI	TTCGAA	1 cut(s)	340
SinI	GGWCC	1 cut(s)	599
SlaI	CTCGAG	1 cut(s)	551
SmiMI	CAYNNNNRTG	1 cut(s)	553
SmlI	CTYRAG	2 cut(s)	395, 551
SmoI	CTYRAG	2 cut(s)	395, 551
Sse9I	AATT	4 cut(s)	92, 180, 298, 743
SsiI	CCGC	5 cut(s)	26, 130, 483, 641, 714
SspDI	GGCGCC	1 cut(s)	107
SspI	AATATT	1 cut(s)	450
SspMI	CTAG	3 cut(s)	660, 686, 855
StyD4I	CCNGG	3 cut(s)	418, 595, 616
StyI	CCWWGG	1 cut(s)	513
TaaI	ACNGT	3 cut(s)	346, 760, 841
TaiI	ACGT	1 cut(s)	276
TaqI	TCGA	5 cut(s)	86, 169, 340, 552, 835
TasI	AATT	4 cut(s)	92, 180, 298, 743
TauI	GCSGC	3 cut(s)	132, 485, 716
TfiI	GAWTC	2 cut(s)	69, 242
Tru1I	TTAA	1 cut(s)	405
Tru9I	TTAA	1 cut(s)	405
TscAI	CASTG	2 cut(s)	466, 825
TseI	GCWGC	5 cut(s)	287, 290, 509, 518, 826
TspDTI	ATGAA	1 cut(s)	736
TspRI	CASTG	2 cut(s)	466, 825
VpaK11BI	GGWCC	1 cut(s)	599
XapI	RAATTY	2 cut(s)	180, 743
XbaI	TCTAGA	1 cut(s)	854
XceI	RCATGY	1 cut(s)	673
XcmI	CCANNNNNNNNNTGG	1 cut(s)	121
XhoI	CTCGAG	1 cut(s)	551
XmiI	GTMKAC	1 cut(s)	86
XspI	CTAG	3 cut(s)	660, 686, 855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.