MD05G1326400.v1.1

PAP-specific phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007801
Physical Location & Seq
Forward (+)
45244569 .. 45247733
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1326400.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGATTTTTGTGCACTCAGCCCGCCATTTTTCGACCGTCCGATTCACTCGGCCCGATTCGGCACTCCGCCGGCGGCGCTTCCGCGTGAGGTCGAGTTTGCCGTTCGGAACTGAAAAGGCCAACTACTATCGAGAGCTGGAAGCTGCCGTCGATGCCGTGGAGAGAGCTTGCGCGCTCTGCGTTGACATCCAGTCGTCGGTGCTTCAACGGCGGGTCGTTGAAAAGACCGACCAGACTCCGGTGACGGTGGCGGATTTTGGAGTCCAGGCTCTCGTCAGTTTGGAGCTTGGGAAACGATTCCCTTTGATTCCATTGGTGGCGGAAGAGGACTCGGCGTTTGTTCGTTCGAATAAACTGGTGGAGCCAGTGCTCAATGCGGTGCTTGACAAATCTAGTTGTGCAGAGAACCCATGGACGGCTGATGAAGTATTGGAAGCCATTGACAGAGGTGGTCCGGAGGGTCTTGCTTTCGGAGCTCAGCCCGCCACGTATTGGGTACTTGACCCCATTGATGGCACAAAAGGATTCGTGAAGGGAAATCCGGCGTTATATGTGGTTGGCTTGGCACTTGTTGTTGACGGGGAGCTTGTATTGGGTGTCATGGGGTGCCCAAACTGGAAGAATGATATATCCAACAAATCCACCTCTGAAGTCCAGGAAGAAAATATCAGTCCACCTGGATCAGGGATTATTATGGTTGCTCATACAGGCTGTGGAACATGGACAAAACGTTTATCATCTGTGCTAAATTCCACAGCCAAAATGCCTTATTCTTGGACTCAATGCTTTGTTGATGGGTCTTGTATCATGGAAGAGGCACGATTTTCTATCCCAGATAGTGATGTATGGGAGTCATTTCCGCTATCATCTTTATTCAGATCAACTAACGCTGATAGCATTGACGAGGGCGAAATTCTTCTTGTAAAATCTTGTTGTGGAAGTTTATCCAAGTATATGATGGTGGCTTCGGGCGTGGTATCTCTTCATATGCAGCGTGTGAAAGTTCACAGAGTTACCAAGGCATGGGATCACGTTGTTGGTATTATATGTGTGCACGAAGCAGGGGGGAAGGTGACTGACTGGAAGGGAGATGAACTTAATCTAGCAGCGGATGAAGTTGGACGGAGGAATATATACCCTTCCGGCGGAATCCTTGCGACTAATGGCACCTTGCATAACCGGCTTTTAGAGATGATATCTTTCAGTTCAACTGTTTCCCGGTGA

Protein Analysis

408

Amino Acids

44.52

Weight (kDa)

5.83

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 70 - 399 3.6e-27 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 606, 1166
AccB7I CCANNNNNTGG 2 cut(s) 492, 1023
AccII CGCG 2 cut(s) 84, 173
AccIII TCCGGA 1 cut(s) 454
AclI AACGTT 1 cut(s) 730
AclWI GGATC 2 cut(s) 688, 1035
AcsI RAATTY 2 cut(s) 748, 912
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 8 cut(s) 196, 238, 415, 492, 512, 683, 1023, 1145
AgsI TTSAA 3 cut(s) 206, 221, 1209
AhdI GACNNNNNGTC 1 cut(s) 190
AjnI CCWGG 3 cut(s) 264, 654, 676
AluBI AGCT 6 cut(s) 136, 143, 167, 286, 476, 586
AluI AGCT 6 cut(s) 136, 143, 167, 286, 476, 586
Alw21I GWGCWC 4 cut(s) 15, 372, 478, 1056
Alw44I GTGCAC 2 cut(s) 11, 1052
AlwI GGATC 2 cut(s) 688, 1035
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 2 cut(s) 50, 117
ApaLI GTGCAC 2 cut(s) 11, 1052
ApeKI GCWGC 3 cut(s) 143, 991, 1106
ApoI RAATTY 2 cut(s) 748, 912
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 2 cut(s) 296, 915
AspLEI GCGC 3 cut(s) 78, 173, 175
AspS9I GGNCC 2 cut(s) 51, 452
AsuC2I CCSGG 1 cut(s) 1219
AsuHPI GGTGA 2 cut(s) 253, 1084
AsuII TTCGAA 1 cut(s) 347
AvaII GGWCC 1 cut(s) 452
BaeGI GKGCMC 3 cut(s) 15, 611, 1056
BanI GGYRCC 2 cut(s) 606, 1166
BanII GRGCYC 1 cut(s) 478
Bbv12I GWGCWC 4 cut(s) 15, 372, 478, 1056
BbvI GCAGC 3 cut(s) 130, 1003, 1118
BccI CCATC 3 cut(s) 506, 788, 952
BceAI ACGGC 5 cut(s) 85, 131, 140, 224, 432
BciT130I CCWGG 3 cut(s) 266, 656, 678
BcnI CCSGG 1 cut(s) 1219
BfaI CTAG 2 cut(s) 393, 1103
BfoI RGCGCY 1 cut(s) 79
BisI GCNGC 4 cut(s) 74, 144, 992, 1107
BlpI GCTNAGC 1 cut(s) 477
BlsI GCNGC 4 cut(s) 75, 145, 993, 1108
Bme1390I CCNGG 4 cut(s) 266, 656, 678, 1219
Bme18I GGWCC 1 cut(s) 452
BmeRI GACNNNNNGTC 1 cut(s) 190
BmgT120I GGNCC 2 cut(s) 51, 452
BmiI GGNNCC 3 cut(s) 363, 608, 1168
BmrFI CCNGG 4 cut(s) 266, 656, 678, 1219
BmsI GCATC 1 cut(s) 142
Bpu1102I GCTNAGC 1 cut(s) 477
Bpu14I TTCGAA 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 1219
BsaAI YACGTR 1 cut(s) 489
BsaJI CCNNGG 3 cut(s) 156, 410, 1017
BsaWI WCCGGW 2 cut(s) 238, 454
Bsc4I CCNNNNNNNGG 8 cut(s) 196, 238, 415, 492, 512, 683, 1023, 1145
Bse118I RCCGGY 2 cut(s) 69, 1179
Bse1I ACTGG 5 cut(s) 190, 360, 365, 620, 1085
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 3 cut(s) 266, 656, 678
BseDI CCNNGG 3 cut(s) 156, 410, 1017
BseGI GGATG 2 cut(s) 186, 1117
BseLI CCNNNNNNNGG 8 cut(s) 196, 238, 415, 492, 512, 683, 1023, 1145
BseMII CTCAG 2 cut(s) 30, 491
BseNI ACTGG 5 cut(s) 190, 360, 365, 620, 1085
BsePI GCGCGC 1 cut(s) 171
BseSI GKGCMC 3 cut(s) 15, 611, 1056
BseXI GCAGC 3 cut(s) 130, 1003, 1118
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 84, 173
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 52, 119
BshNI GGYRCC 2 cut(s) 606, 1166
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 4 cut(s) 15, 372, 478, 1056
BsiSI CCGG 7 cut(s) 70, 239, 455, 542, 1143, 1180, 1219
BslI CCNNNNNNNGG 8 cut(s) 196, 238, 415, 492, 512, 683, 1023, 1145
BsnI GGCC 2 cut(s) 52, 119
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 5 cut(s) 15, 372, 478, 611, 1056
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 3 cut(s) 680, 878, 1027
Bsp1720I GCTNAGC 1 cut(s) 477
Bsp19I CCATGG 1 cut(s) 410
BspANI GGCC 2 cut(s) 52, 119
BspCNI CTCAG 2 cut(s) 29, 490
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 2 cut(s) 84, 173
BspLI GGNNCC 3 cut(s) 363, 608, 1168
BspPI GGATC 2 cut(s) 688, 1035
BspT104I TTCGAA 1 cut(s) 347
BspT107I GGYRCC 2 cut(s) 606, 1166
BsrFI RCCGGY 2 cut(s) 69, 1179
BsrI ACTGG 5 cut(s) 190, 360, 365, 620, 1085
BssAI RCCGGY 2 cut(s) 69, 1179
BssECI CCNNGG 3 cut(s) 156, 410, 1017
BssHII GCGCGC 1 cut(s) 171
BssMI GATC 3 cut(s) 680, 878, 1027
BssT1I CCWWGG 2 cut(s) 410, 1017
Bst2UI CCWGG 3 cut(s) 266, 656, 678
Bst4CI ACNGT 3 cut(s) 37, 247, 1213
Bst6I CTCTTC 3 cut(s) 318, 807, 987
BstBAI YACGTR 1 cut(s) 489
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 5 cut(s) 22, 71, 169, 173, 483
BstDEI CTNAG 2 cut(s) 16, 477
BstDSI CCRYGG 2 cut(s) 156, 410
BstENI CCTNNNNNAGG 1 cut(s) 681
BstF5I GGATG 2 cut(s) 186, 1117
BstFNI CGCG 2 cut(s) 84, 173
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 3 cut(s) 78, 173, 175
BstKTI GATC 3 cut(s) 683, 881, 1030
BstMBI GATC 3 cut(s) 680, 878, 1027
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 7 cut(s) 75, 152, 177, 208, 473, 482, 1180
BstNI CCWGG 3 cut(s) 266, 656, 678
BstSCI CCNGG 4 cut(s) 264, 654, 676, 1217
BstSLI GKGCMC 3 cut(s) 15, 611, 1056
BstUI CGCG 2 cut(s) 84, 173
BstV1I GCAGC 3 cut(s) 130, 1003, 1118
BsuRI GGCC 2 cut(s) 52, 119
BtgI CCRYGG 2 cut(s) 156, 410
BtsCI GGATG 2 cut(s) 186, 1117
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 5 cut(s) 22, 71, 169, 173, 483
CfoI GCGC 3 cut(s) 78, 173, 175
Cfr10I RCCGGY 2 cut(s) 69, 1179
Cfr13I GGNCC 2 cut(s) 51, 452
Csp6I GTAC 1 cut(s) 497
CviAII CATG 5 cut(s) 411, 601, 720, 808, 1023
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 2 cut(s) 16, 477
DpnI GATC 3 cut(s) 682, 880, 1029
DpnII GATC 3 cut(s) 680, 878, 1027
DriI GACNNNNNGTC 1 cut(s) 190
Eam1104I CTCTTC 3 cut(s) 318, 807, 987
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 3 cut(s) 318, 807, 987
EciI GGCGGA 4 cut(s) 56, 266, 335, 1161
Ecl136II GAGCTC 1 cut(s) 476
Eco130I CCWWGG 2 cut(s) 410, 1017
Eco24I GRGCYC 1 cut(s) 478
Eco32I GATATC 1 cut(s) 1197
Eco47I GGWCC 1 cut(s) 452
Eco53kI GAGCTC 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 669
EcoICRI GAGCTC 1 cut(s) 476
EcoNI CCTNNNNNAGG 1 cut(s) 681
EcoRII CCWGG 3 cut(s) 264, 654, 676
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 2 cut(s) 410, 1017
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 410, 1017
FaeI CATG 5 cut(s) 414, 604, 723, 811, 1026
FatI CATG 5 cut(s) 410, 600, 719, 807, 1022
FauI CCCGC 3 cut(s) 29, 204, 490
FauNDI CATATG 1 cut(s) 987
Fnu4HI GCNGC 4 cut(s) 74, 144, 992, 1107
FokI GGATG 2 cut(s) 173, 1124
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 4 cut(s) 74, 144, 992, 1107
FspBI CTAG 2 cut(s) 393, 1103
GlaI GCGC 3 cut(s) 77, 172, 174
GluI GCNGC 4 cut(s) 74, 144, 992, 1107
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 2 cut(s) 52, 119
HapII CCGG 7 cut(s) 70, 239, 455, 542, 1143, 1180, 1219
HhaI GCGC 3 cut(s) 78, 173, 175
Hin1II CATG 5 cut(s) 414, 604, 723, 811, 1026
Hin6I GCGC 3 cut(s) 76, 171, 173
HinP1I GCGC 3 cut(s) 76, 171, 173
HincII GTYRAC 2 cut(s) 184, 577
HindII GTYRAC 2 cut(s) 184, 577
HpaII CCGG 7 cut(s) 70, 239, 455, 542, 1143, 1180, 1219
HphI GGTGA 2 cut(s) 253, 1084
Hpy166II GTNNAC 6 cut(s) 13, 184, 577, 674, 1006, 1054
Hpy188I TCNGA 5 cut(s) 41, 107, 473, 649, 878
Hpy188III TCNNGA 3 cut(s) 131, 455, 529
Hpy8I GTNNAC 6 cut(s) 13, 184, 577, 674, 1006, 1054
Hpy99I CGWCG 2 cut(s) 152, 199
HpyAV CCTTC 4 cut(s) 526, 1063, 1078, 1149
HpyCH4III ACNGT 3 cut(s) 37, 247, 1213
HpyCH4IV ACGT 3 cut(s) 488, 730, 1032
HpyCH4V TGCA 5 cut(s) 13, 401, 991, 1054, 1174
HpyF10VI GCNNNNNNNGC 7 cut(s) 75, 152, 177, 208, 473, 482, 1180
HpyF3I CTNAG 2 cut(s) 16, 477
HpySE526I ACGT 3 cut(s) 488, 730, 1032
Hsp92II CATG 5 cut(s) 414, 604, 723, 811, 1026
HspAI GCGC 3 cut(s) 76, 171, 173
Kpn2I TCCGGA 1 cut(s) 454
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Kzo9I GATC 3 cut(s) 680, 878, 1027
LmnI GCTCC 4 cut(s) 283, 361, 473, 583
Lsp1109I GCAGC 3 cut(s) 130, 1003, 1118
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 393, 1103
MaeII ACGT 3 cut(s) 488, 730, 1032
MaeIII GTNAC 3 cut(s) 241, 1012, 1072
MalI GATC 3 cut(s) 682, 880, 1029
MboI GATC 3 cut(s) 680, 878, 1027
MboII GAAGA 6 cut(s) 335, 631, 671, 824, 908, 974
MhlI GDGCHC 5 cut(s) 15, 372, 478, 611, 1056
MluCI AATT 2 cut(s) 748, 912
MlyI GAGTC 5 cut(s) 229, 270, 323, 772, 860
MmeI TCCRAC 2 cut(s) 657, 1099
MnlI CCTC 8 cut(s) 81, 319, 440, 451, 655, 808, 898, 1119
MreI CGCCGGCG 1 cut(s) 69
MroI TCCGGA 1 cut(s) 454
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 2 cut(s) 296, 915
MseI TTAA 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 1109
MspI CCGG 7 cut(s) 70, 239, 455, 542, 1143, 1180, 1219
MspR9I CCNGG 4 cut(s) 266, 656, 678, 1219
MvaI CCWGG 3 cut(s) 266, 656, 678
MvnI CGCG 2 cut(s) 84, 173
MwoI GCNNNNNNNGC 7 cut(s) 75, 152, 177, 208, 473, 482, 1180
NaeI GCCGGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 1219
NcoI CCATGG 1 cut(s) 410
NdeI CATATG 1 cut(s) 987
NdeII GATC 3 cut(s) 680, 878, 1027
NgoMIV GCCGGC 1 cut(s) 69
NlaIII CATG 5 cut(s) 414, 604, 723, 811, 1026
NlaIV GGNNCC 3 cut(s) 363, 608, 1168
NmeAIII GCCGAG 2 cut(s) 28, 311
NmuCI GTSAC 2 cut(s) 241, 1072
NspV TTCGAA 1 cut(s) 347
PauI GCGCGC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 2 cut(s) 296, 915
PfeI GAWTC 6 cut(s) 42, 56, 297, 307, 525, 1149
PflMI CCANNNNNTGG 2 cut(s) 492, 1023
PfoI TCCNGGA 1 cut(s) 654
PkrI GCNGC 4 cut(s) 75, 145, 993, 1108
PleI GAGTC 5 cut(s) 229, 269, 323, 772, 859
PpsI GAGTC 5 cut(s) 229, 269, 323, 772, 859
Ppu21I YACGTR 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 478
Psp1406I AACGTT 1 cut(s) 730
Psp6I CCWGG 3 cut(s) 264, 654, 676
PspGI CCWGG 3 cut(s) 264, 654, 676
PspN4I GGNNCC 3 cut(s) 363, 608, 1168
PspPI GGNCC 2 cut(s) 51, 452
PteI GCGCGC 1 cut(s) 171
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
SacI GAGCTC 1 cut(s) 478
SaqAI TTAA 1 cut(s) 1098
SatI GCNGC 4 cut(s) 74, 144, 992, 1107
Sau3AI GATC 3 cut(s) 680, 878, 1027
Sau96I GGNCC 2 cut(s) 51, 452
SchI GAGTC 5 cut(s) 229, 270, 323, 772, 860
ScrFI CCNGG 4 cut(s) 266, 656, 678, 1219
SduI GDGCHC 5 cut(s) 15, 372, 478, 611, 1056
SfaNI GCATC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 347
SgrAI CRCCGGYG 1 cut(s) 69
SinI GGWCC 1 cut(s) 452
Sse9I AATT 2 cut(s) 748, 912
SspMI CTAG 2 cut(s) 393, 1103
SstI GAGCTC 1 cut(s) 478
StyD4I CCNGG 4 cut(s) 264, 654, 676, 1217
StyI CCWWGG 2 cut(s) 410, 1017
TaaI ACNGT 3 cut(s) 37, 247, 1213
TaiI ACGT 3 cut(s) 491, 733, 1035
TaqI TCGA 5 cut(s) 32, 92, 130, 150, 347
TaqII GACCGA 1 cut(s) 242
TasI AATT 2 cut(s) 748, 912
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 6 cut(s) 42, 56, 297, 307, 525, 1149
Tru1I TTAA 1 cut(s) 1098
Tru9I TTAA 1 cut(s) 1098
TscAI CASTG 1 cut(s) 372
TseFI GTSAC 2 cut(s) 241, 1072
TseI GCWGC 3 cut(s) 143, 991, 1106
Tsp45I GTSAC 2 cut(s) 241, 1072
TspDTI ATGAA 4 cut(s) 438, 974, 1107, 1128
TspGWI ACGGA 1 cut(s) 1138
TspRI CASTG 1 cut(s) 372
Van91I CCANNNNNTGG 2 cut(s) 492, 1023
VneI GTGCAC 2 cut(s) 11, 1052
VpaK11BI GGWCC 1 cut(s) 452
XagI CCTNNNNNAGG 1 cut(s) 681
XapI RAATTY 2 cut(s) 748, 912
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XmnI GAANNNNTTC 2 cut(s) 296, 915
XspI CTAG 2 cut(s) 393, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.