MD10G1304500.v1.1

PAP-specific phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007801
Physical Location & Seq
Forward (+)
39066072 .. 39068649
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1304500.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGATGGTTTGGTTGGATTTTTTAATTTACTGTATTGTACTGAATTGAGAAACGTAATGCAGGTACTTGACCCCATTGATGGAACAAAAGGATTTGTGAAGGGAAATCCCGCGTTTTTTCAGGTTGGTTTGGCACTTGTTGTTGACGGAAAGGTTGTATTGGGTGTCATGGGCTGCCCGAACTGGAAGAATGATGTATCCAACAAATCCACCTCTGAAGTCCAGGAAGAAAAAAGCAGTCCACCTGGATCAGGGATTATTATGGTTGCACATACAGGCTGTGGCACATGGACAAAACGTTTGTCATCTGTTGTAAATTCCACAGCCAAAATACCTTATTCTTGGACACGATGCTTCGTTGATGGGTCTTGTATCGTGGAAGAGGCACGATTTTCTATCACAGACAGTGATGTATGGGAGTCATTTCCACTATCATCTTTATTCAGCTCCACAACTAATGCTGATAGCATTGACGAGTGTGAAATTCTTCATGTAAAATCTTGTTGTGGAAGTTTATCCAAGTATATAATGGTGGCTTCAGGTGTGGTATCTCTTCATATGCAGCGTGTGAAAGTTCATAAAATTATCAAGGCTTGGGATCACGCTGTTGGTGTTATATGTGTGCATGAAGCAGGGGGAAAGGTGACTGACTGGAAGGGAGATGAACTTAATCTGGCAGCAGATGAAGTTGGACGGAGGAATATATACCCTTCCGGCGGAATCCTTGTGACTAATAGCACCTTGCATAACCGGCTTGTCGAGATGATATCTTCCAGTTCGTCCACTGTTTCGCGGTGA

Protein Analysis

266

Amino Acids

28.68

Weight (kDa)

6.16

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 19 - 257 4.1e-26 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 52
AccII CGCG 2 cut(s) 113, 793
AciI CCGC 3 cut(s) 111, 717, 793
AclI AACGTT 1 cut(s) 298
AclWI GGATC 2 cut(s) 256, 606
AcsI RAATTY 2 cut(s) 316, 483
AcuI CTGAAG 2 cut(s) 237, 522
AfaI GTAC 2 cut(s) 40, 66
AfiI CCNNNNNNNGG 3 cut(s) 80, 251, 716
AjnI CCWGG 2 cut(s) 222, 244
AluBI AGCT 1 cut(s) 447
AluI AGCT 1 cut(s) 447
AlwI GGATC 2 cut(s) 256, 606
ApeKI GCWGC 3 cut(s) 174, 562, 677
ApoI RAATTY 2 cut(s) 316, 483
ArsI GACNNNNNNTTYG 4 cut(s) 283, 287, 315, 319
Asp700I GAANNNNTTC 1 cut(s) 486
AsuHPI GGTGA 1 cut(s) 655
BbvI GCAGC 3 cut(s) 161, 574, 689
BccI CCATC 2 cut(s) 74, 356
BciT130I CCWGG 2 cut(s) 224, 246
BciVI GTATCC 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 52
BfuI GTATCC 1 cut(s) 208
BisI GCNGC 3 cut(s) 175, 563, 678
BlsI GCNGC 3 cut(s) 176, 564, 679
Bme1390I CCNGG 2 cut(s) 224, 246
BmrFI CCNGG 2 cut(s) 224, 246
BmsI GCATC 1 cut(s) 341
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 251, 716
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 3 cut(s) 188, 656, 774
BseBI CCWGG 2 cut(s) 224, 246
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 80, 251, 716
BseNI ACTGG 3 cut(s) 188, 656, 774
BseXI GCAGC 3 cut(s) 161, 574, 689
Bsh1236I CGCG 2 cut(s) 113, 793
BsiSI CCGG 2 cut(s) 714, 751
BslI CCNNNNNNNGG 3 cut(s) 80, 251, 716
Bsp143I GATC 2 cut(s) 248, 598
BspACI CCGC 3 cut(s) 111, 717, 793
BspFNI CGCG 2 cut(s) 113, 793
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 2 cut(s) 256, 606
BsrFI RCCGGY 1 cut(s) 750
BsrI ACTGG 3 cut(s) 188, 656, 774
BssAI RCCGGY 1 cut(s) 750
BssMI GATC 2 cut(s) 248, 598
Bst2UI CCWGG 2 cut(s) 224, 246
Bst4CI ACNGT 3 cut(s) 33, 407, 787
Bst6I CTCTTC 2 cut(s) 375, 558
BstENI CCTNNNNNAGG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 113, 793
BstKTI GATC 2 cut(s) 251, 601
BstMBI GATC 2 cut(s) 248, 598
BstMWI GCNNNNNNNGC 1 cut(s) 751
BstNI CCWGG 2 cut(s) 224, 246
BstSCI CCNGG 2 cut(s) 222, 244
BstUI CGCG 2 cut(s) 113, 793
BstV1I GCAGC 3 cut(s) 161, 574, 689
BsuI GTATCC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 2 cut(s) 412, 783
BveI ACCTGC 1 cut(s) 52
Cfr10I RCCGGY 1 cut(s) 750
Csp6I GTAC 2 cut(s) 39, 65
CviAII CATG 4 cut(s) 169, 288, 491, 626
CviJI RGCY 7 cut(s) 174, 279, 326, 447, 536, 593, 754
CviKI_1 RGCY 7 cut(s) 174, 279, 326, 447, 536, 593, 754
CviQI GTAC 2 cut(s) 39, 65
DpnI GATC 2 cut(s) 250, 600
DpnII GATC 2 cut(s) 248, 598
Eam1104I CTCTTC 2 cut(s) 375, 558
EarI CTCTTC 2 cut(s) 375, 558
EciI GGCGGA 1 cut(s) 732
Eco32I GATATC 1 cut(s) 768
Eco57I CTGAAG 2 cut(s) 237, 522
EcoNI CCTNNNNNAGG 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 222, 244
EcoRV GATATC 1 cut(s) 768
FaeI CATG 4 cut(s) 172, 291, 494, 629
FatI CATG 4 cut(s) 168, 287, 490, 625
FauI CCCGC 1 cut(s) 118
FauNDI CATATG 1 cut(s) 558
Fnu4HI GCNGC 3 cut(s) 175, 563, 678
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 3 cut(s) 175, 563, 678
GluI GCNGC 3 cut(s) 175, 563, 678
HapII CCGG 2 cut(s) 714, 751
Hin1II CATG 4 cut(s) 172, 291, 494, 629
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 2 cut(s) 419, 720
HpaII CCGG 2 cut(s) 714, 751
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 3 cut(s) 145, 242, 783
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 1 cut(s) 760
Hpy8I GTNNAC 3 cut(s) 145, 242, 783
HpyAV CCTTC 3 cut(s) 94, 649, 720
HpyCH4III ACNGT 3 cut(s) 33, 407, 787
HpyCH4IV ACGT 2 cut(s) 54, 298
HpyCH4V TGCA 5 cut(s) 61, 269, 562, 625, 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 751
HpySE526I ACGT 2 cut(s) 54, 298
Hsp92II CATG 4 cut(s) 172, 291, 494, 629
Kzo9I GATC 2 cut(s) 248, 598
LmnI GCTCC 1 cut(s) 452
Lsp1109I GCAGC 3 cut(s) 161, 574, 689
LweI GCATC 1 cut(s) 341
MaeII ACGT 2 cut(s) 54, 298
MaeIII GTNAC 2 cut(s) 643, 727
MalI GATC 2 cut(s) 250, 600
MboI GATC 2 cut(s) 248, 598
MboII GAAGA 6 cut(s) 199, 239, 392, 479, 545, 762
MluCI AATT 5 cut(s) 25, 44, 316, 483, 582
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 2 cut(s) 225, 670
MnlI CCTC 3 cut(s) 223, 376, 690
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 2 cut(s) 24, 669
MspI CCGG 2 cut(s) 714, 751
MspR9I CCNGG 2 cut(s) 224, 246
MvaI CCWGG 2 cut(s) 224, 246
MvnI CGCG 2 cut(s) 113, 793
MwoI GCNNNNNNNGC 1 cut(s) 751
NdeI CATATG 1 cut(s) 558
NdeII GATC 2 cut(s) 248, 598
NlaIII CATG 4 cut(s) 172, 291, 494, 629
NmuCI GTSAC 2 cut(s) 643, 727
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 3 cut(s) 176, 564, 679
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Psp1406I AACGTT 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 222, 244
PspGI CCWGG 2 cut(s) 222, 244
RsaI GTAC 2 cut(s) 40, 66
RsaNI GTAC 2 cut(s) 39, 65
SaqAI TTAA 2 cut(s) 24, 669
SatI GCNGC 3 cut(s) 175, 563, 678
Sau3AI GATC 2 cut(s) 248, 598
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 224, 246
SfaNI GCATC 1 cut(s) 341
Sse9I AATT 5 cut(s) 25, 44, 316, 483, 582
SsiI CCGC 3 cut(s) 111, 717, 793
StyD4I CCNGG 2 cut(s) 222, 244
TaaI ACNGT 3 cut(s) 33, 407, 787
TaiI ACGT 2 cut(s) 57, 301
TaqI TCGA 1 cut(s) 759
TasI AATT 5 cut(s) 25, 44, 316, 483, 582
TatI WGTACW 1 cut(s) 38
TfiI GAWTC 1 cut(s) 720
Tru1I TTAA 2 cut(s) 24, 669
Tru9I TTAA 2 cut(s) 24, 669
TscAI CASTG 2 cut(s) 412, 790
TseFI GTSAC 2 cut(s) 643, 727
TseI GCWGC 3 cut(s) 174, 562, 677
Tsp45I GTSAC 2 cut(s) 643, 727
TspDTI ATGAA 6 cut(s) 479, 545, 566, 642, 678, 699
TspGWI ACGGA 2 cut(s) 162, 709
TspRI CASTG 2 cut(s) 412, 790
XagI CCTNNNNNAGG 1 cut(s) 249
XapI RAATTY 2 cut(s) 316, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.