Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0007351

Physical Location & Seq

Genomic Coordinates Forward (+)

25048428 .. 25053352

Gene Structure 4925 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1111900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2943 bp

ATGTCCGATCCTGAAGACGATACCCAAGCGCCAGCCATTCCGCCGAAGCCAAAGAACGAACGAGAGGCTGCTCTCATGGACTTTGATCTCGACCTCGACGCTTTCTGGCCGTTGGATCCGAATCACTTCCTCTCGAACCCCACATCGCCGCTCTTGTTGCCGTCCAATGACCAGCCCTGCACCCCTTTGTGGGCTTTCCCCGACGGCGATGTCGATGGTGAAGATAAGCTCGCCGTGCATGTTGGTCAAGATCTCTCTGACCCTTCTCAATTCGTTTCCTGTAATTCTAATTCCGTAACACAAGGGTCAACAGAGAACAAGGATAACAGACTGGAACCTGTTGAAAGTTCAGATGGTTATAGTTTATTTAAAGAGAAGATCACTCAAGCACTTAGGTACCTCAAGGAATTGACTGACCAAAATGTTCTAGCTCAGGTTTGGGTACCAGTAAAGGACGGAAATCGGTATGTACTCACAACTTCAGGGCAGCCGTTTGTACTTGATCAACATAGTATTGGACTTCATCAGTATAGGATGGCCTCTCTGATGTATATGTTTTCTGTGGGTGGGGAGAGTGATGGAATGCTTGGGCTTCCTGGCCGCGTTTTCCAGCATAAATTGCCCGAATGGACTCCAAATGTTCAGTACTACTCCATTAAAGAGTATCCACGGCTTGGTCATGCTCAGCATTACAATGTTCAGGGAACCTTAGCTTTGCCCGTGTTTGAGCCCTCTGGACAGTCTTGTGCTGGTGTACTTGAGCTCATAATGACTTCTCCTATTATCAACTGTGGTCCTGAGGTTGATAAAGTTTGCAAAGCACTTGAGGCGGTAAGCTTGAAAAGTTCAGAGATACTGGATCATCCAAGCAGGCAGATTCAAATATGCAATGAAGGTCGCCAAATTGCATTGGCTGAAATCTTGAAGATACTGACAGTGGTATGCGAAACTTACAAATTACCACTTGCTCAGACATGGGTTCCCTGTATGCATCGAAGTGTTTTGGCTTATGGCGGGGGCCTGAAGAAGAGCTGTACTAGCGTTGATGGCAGTTGCATGGAACAGGTCTGCATGTCTACAACTGATGTTGCATTCTACATTATAGATGCTCATATGTGGCGTTTTCGAGATGCCTGCGTTGAACATCACTTACAAAAGGGTCAAGGGGTTGCTGGGAGGGCATTTTCATCCCGTAACGCTTGCTTCTGTGGAGACATTACCCAATTCTGCAAAACTGAGTACCCTTTAGTACACTATGCCCGCATGTTTGGTTTAGCCAGCTCTTTTGCTATCTGCTTACAGAGCACTCATACTGGAAATGATGATTATATTCTAGAATTTTTTCTGCCACCAAGTATCATGGACAGTCATGAACAACAAACTTTGTTGGGCTCCATATTGGCGATAATGAAGAATGATTTCCGGAGTCTCAGTGTTGCTTCTGGTATTGTACTTGAAGAGGAGGGAATAGTTGAAATTGTTCAAGCTTCTACAAATAACGGGCTTGCCACAAGACTTGAATGTATACTGATACCCCATTCTGTGGAATCACCACCAGGGCCTGCCTTACCTAACAGAGAAGAGATGGCACAACTAGATTCATCGAAACCACAGTTAAAGGTAGTATATGATGTTGTAAATGATGGGAGAAATTCTGTTCATGAAGGTGGAGAAAACAATATTTCTCTCCCAGAGAATAAAGACATAAAAAAGAGACCGGAGAAGAAGCGTGGAAAAGCAGAGAAATCAATTAGTCTAGAGGTTCTCCAACAATATTTTGCAGGGAGTCTTAAAGATGCTGCAAAGAATCTTGGAGTTTGCCCCACTACAATGAAGCGCATCTGCAGGCATCATGGAATCTCCCGCTGGCCATCTCGCAAGATCAAGAAGGTCAACCGTTCTCTCACTAAGCTACAACGCGTAATTGAATCAGTTCAAGGTGCTGAAGGAGCATTTGGTTTAACTCCTCTTACTGCAAGTCCACGTCCGGTTGCTGCTGGCTCCATTTCTCAGCCTTATAATTTAAATGGTTCTAATAAACAAGGTTCACCAAGCTCTAAACCTTGTGAAGTTTCTGGAGAAAAGAAAGACTCAACCACCTCTACTTCACCAGGAACAGAGGGCCAGGCTGGGATTGACAATCATTTGTTAGATTGGCGGATACCGAGTCACGAGGAACTTGTTCCAGAACATAACAGGTTTCTGCGAGACAGGGGTAAAGGCTCAAACACATCCAACACAGGAAGCAGCTCAAGGGAGGCCAGTGCTGGTACCCCTACGTCTCATGGTTCATGCCAAGGTAGTCCTGGAAATGGAATTGCCGTGGTAAACTATCCCTTTGTTCCTTTCGTCCATGAACAATGTATTGAAGTAAATGGCTCCCCTCAATCAGCAGTTCGAACACCGGAGGAGTCAAATTTATTAGTTGCATGCTCAATACCCGATGCTCTCCTGATGGCAGAACCTGAGGAACCATGTAGAGGAATGCTATTAGAGGATGCTGGAAGTTCAAAAGATTTGAGAAATCTCTGTCCTTTGGCAGACACAATAATGGACGAGCAAGTTCGAGAAGCTTGTAACACAAACCCTCCATTTTCAGATTCAGCTCCCAAGCAATCCAACACAATGCCAGATATCATGGGCAGGCAAGAAATGAAGAGTGTAACCATAAAGGCGACATATAAAGAGGACATTATTAGGTTTCGTGTGTCGATGAGTTCAGGTATTGTGGAGCTGAAAGAGGCAGTGGCAAAGAGATTAAAACTGGAAGTGGGCACATTCGACATCAAATATGTGGATGATGACCTTGAACGGGTTCTGGTAGCCTGTGATGCAGATCTGCAGGAGTGTATGGAAGTTTGTAGAACATCAGGCAGCAATATGATCAGACTCTCGGTTCATGACATAATGTCCCATCTCGGGAGCTCTTGCGGGAGTGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009889 GO:0009987 GO:0010035 GO:0010118 GO:0010167 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042126 GO:0042128 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0071941 GO:0080090 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001057 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

981

Amino Acids

107.36

Weight (kDa)

5.46

Isoelectric Point (pI)

50.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAF_NLP	PF22922	125 - 261	5.8e-07	NLP protein GAF domain
GAF_NLP	PF22922	294 - 477	1e-76	NLP protein GAF domain
RWP-RK	PF02042	583 - 631	2e-23	RWP-RK domain
PB1	PF00564	888 - 968	3.4e-20	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64530 AT1G64530 AT4G24020 AT4G24020
fragaria_vesca	FvH4_5g11570 FvH4_5g11570
malus_domestica	MD06G1111900.v1.1 MD14G1133200.v1.1 MD14G1133300.v1.1
prunus_persica	Prupe.5G124900_v2.0.a1 Prupe.5G124900_v2.0.a1 Prupe.5G125000_v2.0.a1
pyrus_communis	pycom06g10490
rosa_chinensis	RchiOBHm_Chr7g0186181
rosa_laevigata	RLG00000004858
rosa_multiflora	Rmu_sc0009528.1_g000001
rosa_roxburghii	Rroxscaffold_3G00268340
rosa_rugosa	Rorug06G0472600 Rorug06G0472600 Rorug06G0472600
rosa_samantha	Rh7AG068900 Rh7BG084800 Rh7CG079200 Rh7DG079400
rosa_wichuraiana	Rw7G006610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2019
AasI	GACNNNNNNGTC	1 cut(s)	209
Acc65I	GGTACC	3 cut(s)	396, 442, 2270
AccB1I	GGYRCC	3 cut(s)	396, 442, 2270
AccB7I	CCANNNNNTGG	2 cut(s)	674, 1543
AccBSI	CCGCTC	1 cut(s)	151
AccI	GTMKAC	2 cut(s)	1076, 1525
AccII	CGCG	2 cut(s)	603, 1920
AccIII	TCCGGA	1 cut(s)	1422
AciI	CCGC	9 cut(s)	41, 149, 601, 830, 1014, 1261, 1864, 2158, 2931
AclWI	GGATC	4 cut(s)	2, 110, 123, 867
AcoI	YGGCCR	3 cut(s)	107, 598, 1868
AcsI	RAATTY	3 cut(s)	1337, 1651, 2416
AcuI	CTGAAG	4 cut(s)	33, 465, 1043, 1965
AfiI	CCNNNNNNNGG	8 cut(s)	189, 190, 674, 1543, 2312, 2480, 2812, 2919
AflIII	ACRYGT	1 cut(s)	1918
AhdI	GACNNNNNGTC	1 cut(s)	2908
AjiI	CACGTC	1 cut(s)	1985
AjnI	CCWGG	5 cut(s)	595, 1555, 2110, 2124, 2305
AjuI	GAANNNNNNNTTGG	2 cut(s)	1938, 1970
Alw21I	GWGCWC	3 cut(s)	765, 1307, 2927
Alw26I	GTCTC	5 cut(s)	1206, 1433, 1708, 2202, 2286
AlwI	GGATC	4 cut(s)	2, 110, 123, 867
AlwNI	CAGNNNCTG	2 cut(s)	1562, 2465
Ama87I	CYCGRG	1 cut(s)	2918
Aor13HI	TCCGGA	1 cut(s)	1422
AoxI	GGCC	8 cut(s)	107, 537, 598, 1018, 1559, 1868, 2122, 2259
ApeKI	GCWGC	6 cut(s)	68, 487, 1799, 1994, 2247, 2874
ApoI	RAATTY	3 cut(s)	1337, 1651, 2416
Asp700I	GAANNNNTTC	7 cut(s)	125, 1341, 1418, 1479, 1932, 2181, 2814
Asp718I	GGTACC	3 cut(s)	396, 442, 2270
AspLEI	GCGC	2 cut(s)	31, 1839
AspS9I	GGNCC	4 cut(s)	794, 1018, 1559, 2122
AsuHPI	GGTGA	4 cut(s)	230, 1542, 2040, 2100
AsuII	TTCGAA	1 cut(s)	2398
AvaI	CYCGRG	1 cut(s)	2918
AvaII	GGWCC	1 cut(s)	794
AxyI	CCTNAGG	2 cut(s)	798, 2466
BaeGI	GKGCMC	1 cut(s)	2777
BaeI	ACNNNNGTAYC	2 cut(s)	380, 413
BalI	TGGCCA	1 cut(s)	1870
BamHI	GGATCC	1 cut(s)	115
BanI	GGYRCC	3 cut(s)	396, 442, 2270
BanII	GRGCYC	4 cut(s)	732, 765, 1394, 2927
BauI	CACGAG	1 cut(s)	2171
BbsI	GAAGAC	1 cut(s)	21
Bbv12I	GWGCWC	3 cut(s)	765, 1307, 2927
BbvI	GCAGC	6 cut(s)	55, 499, 1786, 1981, 2259, 2886
BceAI	ACGGC	7 cut(s)	94, 145, 218, 220, 475, 686, 2306
BcgI	CGANNNNNNTGC	2 cut(s)	1116, 1150
BciT130I	CCWGG	5 cut(s)	597, 1557, 2112, 2126, 2307
BciVI	GTATCC	2 cut(s)	675, 2154
BclI	TGATCA	2 cut(s)	502, 2883
BcoDI	GTCTC	5 cut(s)	1206, 1433, 1708, 2202, 2286
BfaI	CTAG	5 cut(s)	428, 1038, 1334, 1595, 1757
BfmI	CTRYAG	2 cut(s)	1843, 2840
BfoI	RGCGCY	1 cut(s)	32
BfuI	GTATCC	2 cut(s)	675, 2154
BglII	AGATCT	2 cut(s)	250, 2836
BisI	GCNGC	8 cut(s)	69, 149, 488, 601, 1800, 1995, 2248, 2875
BlpI	GCTNAGC	1 cut(s)	684
BlsI	GCNGC	8 cut(s)	70, 150, 489, 602, 1801, 1996, 2249, 2876
BmcAI	AGTACT	1 cut(s)	647
Bme1390I	CCNGG	5 cut(s)	597, 1557, 2112, 2126, 2307
Bme18I	GGWCC	1 cut(s)	794
BmeRI	GACNNNNNGTC	1 cut(s)	2908
BmeT110I	CYCGRG	1 cut(s)	2918
BmgBI	CACGTC	1 cut(s)	1985
BmgT120I	GGNCC	4 cut(s)	794, 1018, 1559, 2122
BmrFI	CCNGG	5 cut(s)	597, 1557, 2112, 2126, 2307
BmsI	GCATC	9 cut(s)	1000, 1096, 1120, 1786, 1848, 1858, 2434, 2488, 2821
BpiI	GAAGAC	1 cut(s)	21
BpmI	CTGGAG	1 cut(s)	2097
Bpu10I	CCTNAGC	2 cut(s)	432, 709
Bpu1102I	GCTNAGC	1 cut(s)	684
Bpu14I	TTCGAA	1 cut(s)	2398
BpuEI	CTTGAG	5 cut(s)	369, 386, 779, 845, 2236
BsaBI	GATNNNNATC	2 cut(s)	120, 2835
BsaI	GGTCTC	1 cut(s)	1708
BsaJI	CCNNGG	4 cut(s)	668, 1556, 2296, 2322
BsaWI	WCCGGW	4 cut(s)	1422, 1717, 1987, 2404
BsaXI	ACNNNNNCTCC	4 cut(s)	1417, 1447, 2572, 2602
Bsc4I	CCNNNNNNNGG	8 cut(s)	189, 190, 674, 1543, 2312, 2480, 2812, 2919
Bse1I	ACTGG	6 cut(s)	336, 446, 861, 1318, 2262, 2769
Bse21I	CCTNAGG	2 cut(s)	798, 2466
Bse3DI	GCAATG	1 cut(s)	895
Bse8I	GATNNNNATC	2 cut(s)	120, 2835
BseAI	TCCGGA	1 cut(s)	1422
BseBI	CCWGG	5 cut(s)	597, 1557, 2112, 2126, 2307
BseDI	CCNNGG	4 cut(s)	668, 1556, 2296, 2322
BseGI	GGATG	6 cut(s)	540, 862, 1187, 2231, 2503, 2803
BseJI	GATNNNNATC	2 cut(s)	120, 2835
BseLI	CCNNNNNNNGG	8 cut(s)	189, 190, 674, 1543, 2312, 2480, 2812, 2919
BseMI	GCAATG	1 cut(s)	895
BseMII	CTCAG	8 cut(s)	446, 698, 789, 983, 1227, 1444, 2024, 2457
BseNI	ACTGG	6 cut(s)	336, 446, 861, 1318, 2262, 2769
BseRI	GAGGAG	3 cut(s)	1475, 1956, 2423
BseSI	GKGCMC	1 cut(s)	2777
BseXI	GCAGC	6 cut(s)	55, 499, 1786, 1981, 2259, 2886
BseYI	CCCAGC	2 cut(s)	1172, 2129
BsgI	GTGCAG	1 cut(s)	163
Bsh1236I	CGCG	2 cut(s)	603, 1920
BshFI	GGCC	8 cut(s)	109, 539, 600, 1020, 1561, 1870, 2124, 2261
BshNI	GGYRCC	3 cut(s)	396, 442, 2270
BsiHKAI	GWGCWC	3 cut(s)	765, 1307, 2927
BsiHKCI	CYCGRG	1 cut(s)	2918
BsiSI	CCGG	4 cut(s)	1423, 1718, 1988, 2405
BslFI	GGGAC	1 cut(s)	2896
BslI	CCNNNNNNNGG	8 cut(s)	189, 190, 674, 1543, 2312, 2480, 2812, 2919
BsmAI	GTCTC	5 cut(s)	1206, 1433, 1708, 2202, 2286
BsmBI	CGTCTC	1 cut(s)	2286
BsmFI	GGGAC	1 cut(s)	2896
BsmI	GAATGC	3 cut(s)	588, 1091, 2490
BsnI	GGCC	8 cut(s)	109, 539, 600, 1020, 1561, 1870, 2124, 2261
Bso31I	GGTCTC	1 cut(s)	1708
BsoBI	CYCGRG	1 cut(s)	2918
Bsp119I	TTCGAA	1 cut(s)	2398
Bsp1286I	GDGCHC	6 cut(s)	732, 765, 1307, 1394, 2777, 2927
Bsp13I	TCCGGA	1 cut(s)	1422
Bsp1720I	GCTNAGC	1 cut(s)	684
BspACI	CCGC	9 cut(s)	41, 149, 601, 830, 1014, 1261, 1864, 2158, 2931
BspANI	GGCC	8 cut(s)	109, 539, 600, 1020, 1561, 1870, 2124, 2261
BspCNI	CTCAG	8 cut(s)	445, 697, 790, 982, 1228, 1443, 2023, 2458
BspEI	TCCGGA	1 cut(s)	1422
BspFNI	CGCG	2 cut(s)	603, 1920
BspHI	TCATGA	3 cut(s)	1369, 1660, 2899
BspMAI	CTGCAG	2 cut(s)	1847, 2844
BspPI	GGATC	4 cut(s)	2, 110, 123, 867
BspQI	GCTCTTC	1 cut(s)	1022
BspT104I	TTCGAA	1 cut(s)	2398
BspT107I	GGYRCC	3 cut(s)	396, 442, 2270
BspTNI	GGTCTC	1 cut(s)	1708
BsrBI	CCGCTC	1 cut(s)	151
BsrDI	GCAATG	1 cut(s)	895
BsrI	ACTGG	6 cut(s)	336, 446, 861, 1318, 2262, 2769
BssECI	CCNNGG	4 cut(s)	668, 1556, 2296, 2322
BssNAI	GTATAC	1 cut(s)	1526
BssSI	CACGAG	1 cut(s)	2171
BssT1I	CCWWGG	1 cut(s)	2296
Bst1107I	GTATAC	1 cut(s)	1526
Bst2BI	CACGAG	1 cut(s)	2171
Bst2UI	CCWGG	5 cut(s)	597, 1557, 2112, 2126, 2307
Bst4CI	ACNGT	6 cut(s)	741, 791, 937, 1367, 1614, 1898
Bst6I	CTCTTC	4 cut(s)	1022, 1452, 1575, 2651
BstAPI	GCANNNNNTGC	1 cut(s)	619
BstBI	TTCGAA	1 cut(s)	2398
BstDSI	CCRYGG	2 cut(s)	668, 2322
BstF5I	GGATG	6 cut(s)	540, 862, 1187, 2231, 2503, 2803
BstFNI	CGCG	2 cut(s)	603, 1920
BstH2I	RGCGCY	1 cut(s)	32
BstHHI	GCGC	2 cut(s)	31, 1839
BstMAI	GTCTC	5 cut(s)	1206, 1433, 1708, 2202, 2286
BstNI	CCWGG	5 cut(s)	597, 1557, 2112, 2126, 2307
BstNSI	RCATGY	4 cut(s)	242, 1075, 1267, 2433
BstSCI	CCNGG	5 cut(s)	595, 1555, 2110, 2124, 2305
BstSFI	CTRYAG	2 cut(s)	1843, 2840
BstSLI	GKGCMC	1 cut(s)	2777
BstUI	CGCG	2 cut(s)	603, 1920
BstV1I	GCAGC	6 cut(s)	55, 499, 1786, 1981, 2259, 2886
BstV2I	GAAGAC	1 cut(s)	21
BstX2I	RGATCY	3 cut(s)	115, 250, 2836
BstYI	RGATCY	3 cut(s)	115, 250, 2836
BstZ17I	GTATAC	1 cut(s)	1526
Bsu36I	CCTNAGG	2 cut(s)	798, 2466
BsuI	GTATCC	2 cut(s)	675, 2154
BsuRI	GGCC	8 cut(s)	109, 539, 600, 1020, 1561, 1870, 2124, 2261
BtgI	CCRYGG	2 cut(s)	668, 2322
BtgZI	GCGATG	2 cut(s)	129, 222
BtrI	CACGTC	1 cut(s)	1985
BtsCI	GGATG	6 cut(s)	540, 862, 1187, 2231, 2503, 2803
BtsI	GCAGTG	1 cut(s)	2751
BtsIMutI	CAGTG	4 cut(s)	942, 1438, 2269, 2751
CaiI	CAGNNNCTG	2 cut(s)	1562, 2465
CciI	TCATGA	3 cut(s)	1369, 1660, 2899
CfoI	GCGC	2 cut(s)	31, 1839
Cfr13I	GGNCC	4 cut(s)	794, 1018, 1559, 2122
CseI	GACGC	1 cut(s)	107
CspCI	CAANNNNNGTGG	2 cut(s)	1498, 1533
DraI	TTTAAA	2 cut(s)	370, 2025
DrdI	GACNNNNNNGTC	1 cut(s)	209
DriI	GACNNNNNGTC	1 cut(s)	2908
DseDI	GACNNNNNNGTC	1 cut(s)	209
EaeI	YGGCCR	3 cut(s)	107, 598, 1868
Eam1104I	CTCTTC	4 cut(s)	1022, 1452, 1575, 2651
Eam1105I	GACNNNNNGTC	1 cut(s)	2908
EarI	CTCTTC	4 cut(s)	1022, 1452, 1575, 2651
EciI	GGCGGA	2 cut(s)	30, 2173
Ecl136II	GAGCTC	2 cut(s)	763, 2925
Eco130I	CCWWGG	1 cut(s)	2296
Eco24I	GRGCYC	4 cut(s)	732, 765, 1394, 2927
Eco31I	GGTCTC	1 cut(s)	1708
Eco32I	GATATC	1 cut(s)	2635
Eco47I	GGWCC	1 cut(s)	794
Eco53kI	GAGCTC	2 cut(s)	763, 2925
Eco57I	CTGAAG	4 cut(s)	33, 465, 1043, 1965
Eco81I	CCTNAGG	2 cut(s)	798, 2466
Eco88I	CYCGRG	1 cut(s)	2918
EcoICRI	GAGCTC	2 cut(s)	763, 2925
EcoO109I	RGGNCCY	2 cut(s)	1018, 1559
EcoRII	CCWGG	5 cut(s)	595, 1555, 2110, 2124, 2305
EcoRV	GATATC	1 cut(s)	2635
EcoT14I	CCWWGG	1 cut(s)	2296
EcoT22I	ATGCAT	1 cut(s)	993
EcoT38I	GRGCYC	4 cut(s)	732, 765, 1394, 2927
ErhI	CCWWGG	1 cut(s)	2296
Esp3I	CGTCTC	1 cut(s)	2286
FaqI	GGGAC	1 cut(s)	2896
FauI	CCCGC	4 cut(s)	1007, 1268, 1871, 2924
FauNDI	CATATG	1 cut(s)	1113
FbaI	TGATCA	2 cut(s)	502, 2883
FblI	GTMKAC	2 cut(s)	1076, 1525
Fnu4HI	GCNGC	8 cut(s)	69, 149, 488, 601, 1800, 1995, 2248, 2875
FokI	GGATG	6 cut(s)	547, 849, 1174, 2218, 2510, 2810
FriOI	GRGCYC	4 cut(s)	732, 765, 1394, 2927
Fsp4HI	GCNGC	8 cut(s)	69, 149, 488, 601, 1800, 1995, 2248, 2875
FspBI	CTAG	5 cut(s)	428, 1038, 1334, 1595, 1757
GlaI	GCGC	2 cut(s)	30, 1838
GluI	GCNGC	8 cut(s)	69, 149, 488, 601, 1800, 1995, 2248, 2875
GsaI	CCCAGC	2 cut(s)	1176, 2133
GsuI	CTGGAG	1 cut(s)	2097
HaeII	RGCGCY	1 cut(s)	32
HaeIII	GGCC	8 cut(s)	109, 539, 600, 1020, 1561, 1870, 2124, 2261
HapII	CCGG	4 cut(s)	1423, 1718, 1988, 2405
HgaI	GACGC	1 cut(s)	107
HhaI	GCGC	2 cut(s)	31, 1839
Hin6I	GCGC	2 cut(s)	29, 1837
HinP1I	GCGC	2 cut(s)	29, 1837
HincII	GTYRAC	2 cut(s)	309, 1894
HindII	GTYRAC	2 cut(s)	309, 1894
HindIII	AAGCTT	3 cut(s)	835, 1485, 2571
HpaII	CCGG	4 cut(s)	1423, 1718, 1988, 2405
HphI	GGTGA	4 cut(s)	230, 1542, 2040, 2100
Hpy166II	GTNNAC	9 cut(s)	309, 755, 1077, 1252, 1526, 1894, 1982, 2048, 2329
Hpy188I	TCNGA	9 cut(s)	7, 120, 259, 352, 546, 850, 972, 2599, 2888
Hpy8I	GTNNAC	9 cut(s)	309, 755, 1077, 1252, 1526, 1894, 1982, 2048, 2329
Hpy99I	CGWCG	2 cut(s)	101, 206
HpyAV	CCTTC	5 cut(s)	273, 887, 1658, 1882, 1940
HpyCH4III	ACNGT	6 cut(s)	741, 791, 937, 1367, 1614, 1898
HpyCH4IV	ACGT	2 cut(s)	1984, 2279
HpySE526I	ACGT	2 cut(s)	1984, 2279
HspAI	GCGC	2 cut(s)	29, 1837
Kpn2I	TCCGGA	1 cut(s)	1422
KpnI	GGTACC	3 cut(s)	400, 446, 2274
Ksp22I	TGATCA	2 cut(s)	502, 2883
LguI	GCTCTTC	1 cut(s)	1022
LmnI	GCTCC	7 cut(s)	1397, 1949, 2006, 2384, 2611, 2731, 2922
Lsp1109I	GCAGC	6 cut(s)	55, 499, 1786, 1981, 2259, 2886
LweI	GCATC	9 cut(s)	1000, 1096, 1120, 1786, 1848, 1858, 2434, 2488, 2821
MaeI	CTAG	5 cut(s)	428, 1038, 1334, 1595, 1757
MaeII	ACGT	2 cut(s)	1984, 2279
MaeIII	GTNAC	5 cut(s)	295, 1193, 2168, 2576, 2662
MbiI	CCGCTC	1 cut(s)	151
MflI	RGATCY	3 cut(s)	115, 250, 2836
MhlI	GDGCHC	6 cut(s)	732, 765, 1307, 1394, 2777, 2927
MlsI	TGGCCA	1 cut(s)	1870
MluI	ACGCGT	1 cut(s)	1918
MluNI	TGGCCA	1 cut(s)	1870
MlyI	GAGTC	7 cut(s)	625, 1435, 1795, 2084, 2176, 2420, 2883
MmeI	TCCRAC	4 cut(s)	93, 1792, 2259, 2643
Mox20I	TGGCCA	1 cut(s)	1870
Mph1103I	ATGCAT	1 cut(s)	993
MroI	TCCGGA	1 cut(s)	1422
MroXI	GAANNNNTTC	7 cut(s)	125, 1341, 1418, 1479, 1932, 2181, 2814
MscI	TGGCCA	1 cut(s)	1870
MseI	TTAA	7 cut(s)	369, 657, 1616, 1791, 1961, 2024, 2759
MslI	CAYNNNNRTG	5 cut(s)	693, 996, 1665, 1829, 2549
Msp20I	TGGCCA	1 cut(s)	1870
MspA1I	CMGCKG	1 cut(s)	1866
MspI	CCGG	4 cut(s)	1423, 1718, 1988, 2405
MspR9I	CCNGG	5 cut(s)	597, 1557, 2112, 2126, 2307
Mva1269I	GAATGC	3 cut(s)	588, 1091, 2490
MvaI	CCWGG	5 cut(s)	597, 1557, 2112, 2126, 2307
MvnI	CGCG	2 cut(s)	603, 1920
NdeI	CATATG	1 cut(s)	1113
NmuCI	GTSAC	1 cut(s)	2168
NsiI	ATGCAT	1 cut(s)	993
NspI	RCATGY	4 cut(s)	242, 1075, 1267, 2433
NspV	TTCGAA	1 cut(s)	2398
PaeI	GCATGC	1 cut(s)	2433
PagI	TCATGA	3 cut(s)	1369, 1660, 2899
PciSI	GCTCTTC	1 cut(s)	1022
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PctI	GAATGC	3 cut(s)	588, 1091, 2490
PdmI	GAANNNNTTC	7 cut(s)	125, 1341, 1418, 1479, 1932, 2181, 2814
PfeI	GAWTC	8 cut(s)	121, 877, 1547, 1598, 1807, 1857, 1928, 2600
PflMI	CCANNNNNTGG	2 cut(s)	674, 1543
PfoI	TCCNGGA	1 cut(s)	2305
PkrI	GCNGC	8 cut(s)	70, 150, 489, 602, 1801, 1996, 2249, 2876
PleI	GAGTC	7 cut(s)	625, 1434, 1794, 2084, 2175, 2419, 2883
PpsI	GAGTC	7 cut(s)	625, 1434, 1794, 2084, 2175, 2419, 2883
PsiI	TTATAA	1 cut(s)	2019
Psp124BI	GAGCTC	2 cut(s)	765, 2927
Psp6I	CCWGG	5 cut(s)	595, 1555, 2110, 2124, 2305
PspFI	CCCAGC	2 cut(s)	1172, 2129
PspGI	CCWGG	5 cut(s)	595, 1555, 2110, 2124, 2305
PspPI	GGNCC	4 cut(s)	794, 1018, 1559, 2122
PsrI	GAACNNNNNNTAC	2 cut(s)	1134, 1166
PstI	CTGCAG	2 cut(s)	1847, 2844
PstNI	CAGNNNCTG	2 cut(s)	1562, 2465
PsuI	RGATCY	3 cut(s)	115, 250, 2836
RseI	CAYNNNNRTG	5 cut(s)	693, 996, 1665, 1829, 2549
SacI	GAGCTC	2 cut(s)	765, 2927
SapI	GCTCTTC	1 cut(s)	1022
SaqAI	TTAA	7 cut(s)	369, 657, 1616, 1791, 1961, 2024, 2759
SatI	GCNGC	8 cut(s)	69, 149, 488, 601, 1800, 1995, 2248, 2875
Sau96I	GGNCC	4 cut(s)	794, 1018, 1559, 2122
ScaI	AGTACT	1 cut(s)	647
SchI	GAGTC	7 cut(s)	625, 1435, 1795, 2084, 2176, 2420, 2883
ScrFI	CCNGG	5 cut(s)	597, 1557, 2112, 2126, 2307
SduI	GDGCHC	6 cut(s)	732, 765, 1307, 1394, 2777, 2927
SfaNI	GCATC	9 cut(s)	1000, 1096, 1120, 1786, 1848, 1858, 2434, 2488, 2821
SfcI	CTRYAG	2 cut(s)	1843, 2840
SfuI	TTCGAA	1 cut(s)	2398
SinI	GGWCC	1 cut(s)	794
SmiI	ATTTAAAT	1 cut(s)	2025
SmiMI	CAYNNNNRTG	5 cut(s)	693, 996, 1665, 1829, 2549
SmlI	CTYRAG	5 cut(s)	384, 401, 758, 824, 2251
SmoI	CTYRAG	5 cut(s)	384, 401, 758, 824, 2251
SphI	GCATGC	1 cut(s)	2433
SsiI	CCGC	9 cut(s)	41, 149, 601, 830, 1014, 1261, 1864, 2158, 2931
SspI	AATATT	2 cut(s)	1681, 1775
SspMI	CTAG	5 cut(s)	428, 1038, 1334, 1595, 1757
SstI	GAGCTC	2 cut(s)	765, 2927
StyD4I	CCNGG	5 cut(s)	595, 1555, 2110, 2124, 2305
StyI	CCWWGG	1 cut(s)	2296
SwaI	ATTTAAAT	1 cut(s)	2025
TaaI	ACNGT	6 cut(s)	741, 791, 937, 1367, 1614, 1898
TaiI	ACGT	2 cut(s)	1987, 2282
TatI	WGTACW	7 cut(s)	469, 496, 645, 754, 1034, 1249, 1450
TauI	GCSGC	2 cut(s)	151, 603
TfiI	GAWTC	8 cut(s)	121, 877, 1547, 1598, 1807, 1857, 1928, 2600
Tru1I	TTAA	7 cut(s)	369, 657, 1616, 1791, 1961, 2024, 2759
Tru9I	TTAA	7 cut(s)	369, 657, 1616, 1791, 1961, 2024, 2759
TscAI	CASTG	4 cut(s)	942, 1438, 2269, 2751
TseFI	GTSAC	1 cut(s)	2168
TseI	GCWGC	6 cut(s)	68, 487, 1799, 1994, 2247, 2874
Tsp45I	GTSAC	1 cut(s)	2168
TspGWI	ACGGA	2 cut(s)	283, 471
TspRI	CASTG	4 cut(s)	942, 1438, 2269, 2751
Van91I	CCANNNNNTGG	2 cut(s)	674, 1543
VpaK11BI	GGWCC	1 cut(s)	794
XapI	RAATTY	3 cut(s)	1337, 1651, 2416
XbaI	TCTAGA	2 cut(s)	1333, 1756
XceI	RCATGY	4 cut(s)	242, 1075, 1267, 2433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2303
XmiI	GTMKAC	2 cut(s)	1076, 1525
XmnI	GAANNNNTTC	7 cut(s)	125, 1341, 1418, 1479, 1932, 2181, 2814
XspI	CTAG	5 cut(s)	428, 1038, 1334, 1595, 1757
ZrmI	AGTACT	1 cut(s)	647
Zsp2I	ATGCAT	1 cut(s)	993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.