MD06G1177100.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003359
Physical Location & Seq
Reverse (-)
31692100 .. 31694163
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1177100.v1.1.491

Sequence Viewer

Length: 2064 bp
ATGAACCCGAAATTCGACAGCAGCGACCGCCGGATTCTCACGTTTCCGGCGGTGCGACCTTGCGAATTTATTTCTCTGGAGACCCTTCTCGAGTCTCTGATCGCTCTCTCCCACACCATCTGCAGTTTCCAATCGAAATGCTGTGCGACGCAATGGAGGAACAGCCGGGAAGCAATTCGGCAGGTTCGTGTTCTTCTCATGTTCTTTGAGGAGGTACGTGAATGCAATTCGGTTGTTCCGAAATCGTCTGTTGTCGTCTGCCTCTCCGAGCTTCATTTCGTCTTCCAGAAAATTCGGTTTTTATTGGAGGATTGTATGAGGGAAGGTGGTCGAGTTTTGATGCTGACGAAATGCGAGTTTCTTGCGACTCAATTTCGGTTTCTGATTCGAGCTGTGGCGACGGCTCTCGATGTTCTGCCGTTGAAAGTGATCGATGTTGGCGATGAAGTTAAGGAATTGGTTGAATTGGTGTCGAAGCAATCGTGGAAGGCCAACTTAGAGCTCCAAGTGGGTGACAGATTGGCTTCGAAACGATTGCTTGCGATTCTGAACCAATTCGAAAAGGGAATCGAGCCCGATTTGATTGCCGTAAAGCGGGTCCTCGATTACCTTCGAATTAAAAGCTGGAGCGAGTGCAACACGGAGATCAAATTCTTGGAAGAACAAATTGCTATCAAGCGTTTCGATCAGGAGGAGATGGATGTTCCTTTTCTGAGTAGCTTGGTGGGGTTCATGAGCTACAGCAGGGGGCTGATTTTCGAAACATTGGATCATCAAAACCCCGCGAATCAAATCGATTCCAGGTGCGACGTGGAGATGTTTCGTTGCTTAAACCCTGAAGATTTCAGGTGCCCGATTTCTCTGGAGCTAATGCTTGATCCGGTGACTGTATCTACAGGTCAGACGTACGATCGAAATTCGATTCAAAAATGGCTCGACGACGGACACATGCTCTGCCCCAAAACAGGAGAGATGATCACAAACACAGAGTTGGTACCGAATTCGGTTCTCCGAAGGCTGATAAAAAACCTTTGTGAATACAACGGCATTTCTCTCGGCAGGCCAGGAAGGAAAAGCGCCGACAAGGAAAAAACGATCGCACCTGGTAGCCCGGTTGCTAAACACGCAATGAGGTTTCTGTCGAGATTTCTCAGCGGAAGGCTTGCTTGCGGAACAAGCGAACAGCAAAACAAGGCCGCTTATGAGATTCGATTGCTAGGCAAATCAAACATTTTCAACAGGTCTTGTTTGATCAAGGCTGGCTGTATCCCTCCTCTGCTGAGGCTACTGAATTCCGCTGATGCATCCACGCAAGGGAATGCAATTGCAGCCTTGTTGAAGCTCGCGAAATACGCAAGCGGAATGAAATTGATAATGTCATGGGGAGGCCTACCATCAGTTGTTGCTGTTCTTAAAAATGGGTTGAGTTTAGAAGCCCGACAAAGCGCAGCTGCAACGATGTTTTACCTCTCTTCGATGCGGGAGTCTCGGAAACTAATCGGAGAAATGCCTGAGGCCATCCCAGCTCTTGTGGAGCTGATCAGGGAAGGAAATACGTGCGGGAAAAAGAACTCCGTGGTTGCGATTTTCGGGCTGATCCTCCTTGCTAGGAATCAACAAAAAGTGCTGGACGCGGGCATTGTTCCACTGCTAGTTGACATAATATCTTCTTCGTCACAAAACCCTGATCAGCTCGTTGATGATGCATTATCAGTTCTTGCAATTTTGGCTGAAAATGTCGTAGGATCATACGAAATCCTCCACTCCATTGATTTGCAATTGATCATCCGCATTCTTCAAACTTCGACATCCCCAAAAGCAAGAGAGTATTGTGTTTCAACACTGCTGTCTTTGTGCATCAATGGCGGGGCGGAAGTTGTTGCTGTTTTGGCAACGGAATCATCACTAATGCCTAGACTCTTTTCGCTCTTGACGGATGGAACATCTTATGCGATCAAGAAATCGCGCTCCCTCATCAAAATTCTCCATGACTTTCGTGAAACTAGTACATCAAGATGGATGAGTTCTGCAGTTAGGCGCAAACAAGGCGTCCAGGAACGGTGA

Protein Analysis

688

Amino Acids

76.7

Weight (kDa)

8.7

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB10_N PF25368 59 - 160 3.2e-11 PUB10-like, N-terminal domain
U-box PF04564 279 - 343 3e-16 U-box domain
ARM_PUB PF25598 394 - 664 3.3e-15 Plant U-box protein ARM repeat region
Arm PF00514 413 - 449 1.3e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 413 - 617 4e-10 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 172
Acc65I GGTACC 1 cut(s) 994
AccB1I GGYRCC 2 cut(s) 849, 994
AccII CGCG 4 cut(s) 785, 1346, 1634, 1966
AclWI GGATC 4 cut(s) 777, 872, 1591, 1753
AcsI RAATTY 8 cut(s) 11, 65, 291, 650, 916, 1000, 1291, 1980
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 1 cut(s) 2049
AfaI GTAC 4 cut(s) 216, 908, 996, 2008
AfiI CCNNNNNNNGG 3 cut(s) 594, 965, 1314
AgsI TTSAA 7 cut(s) 424, 464, 926, 1237, 1339, 1799, 1839
AhlI ACTAGT 1 cut(s) 2003
AjiI CACGTC 1 cut(s) 811
AjnI CCWGG 4 cut(s) 800, 1063, 1102, 2052
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 3 cut(s) 74, 99, 1491
AlwI GGATC 4 cut(s) 777, 872, 1591, 1753
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 5 cut(s) 489, 1061, 1194, 1387, 1515
ApeKI GCWGC 4 cut(s) 21, 1328, 1448, 1451
ApoI RAATTY 8 cut(s) 11, 65, 291, 650, 916, 1000, 1291, 1980
ArsI GACNNNNNNTTYG 1 cut(s) 2034
Asp700I GAANNNNTTC 2 cut(s) 174, 554
Asp718I GGTACC 1 cut(s) 994
AspLEI GCGC 4 cut(s) 1079, 1448, 1968, 2040
AspS9I GGNCC 1 cut(s) 598
AsuC2I CCSGG 2 cut(s) 167, 1112
AsuHPI GGTGA 2 cut(s) 524, 895
AsuII TTCGAA 4 cut(s) 527, 558, 613, 759
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 598
AxyI CCTNAGG 1 cut(s) 1512
BaeGI GKGCMC 1 cut(s) 854
BanI GGYRCC 2 cut(s) 849, 994
BanII GRGCYC 2 cut(s) 504, 576
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 504
BbvCI CCTCAGC 1 cut(s) 1280
BbvI GCAGC 4 cut(s) 33, 1340, 1438, 1460
BccI CCATC 6 cut(s) 125, 691, 1402, 1526, 1931, 2010
BceAI ACGGC 4 cut(s) 403, 417, 572, 1060
BcgI CGANNNNNNTGC 8 cut(s) 344, 378, 517, 551, 566, 600, 1036, 1070
BciT130I CCWGG 4 cut(s) 802, 1065, 1104, 2054
BciVI GTATCC 1 cut(s) 1277
BclI TGATCA 5 cut(s) 975, 1251, 1539, 1687, 1782
BcnI CCSGG 2 cut(s) 167, 1112
BcoDI GTCTC 3 cut(s) 74, 99, 1491
BcuI ACTAGT 1 cut(s) 2003
BfaI CTAG 5 cut(s) 1217, 1608, 1652, 1914, 2004
BfmI CTRYAG 4 cut(s) 121, 739, 894, 2028
BfoI RGCGCY 1 cut(s) 1080
BfuAI ACCTGC 1 cut(s) 172
BfuI GTATCC 1 cut(s) 1277
BisI GCNGC 5 cut(s) 22, 1197, 1329, 1449, 1452
BlsI GCNGC 5 cut(s) 23, 1198, 1330, 1450, 1453
Bme1390I CCNGG 6 cut(s) 167, 802, 1065, 1104, 1112, 2054
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 89
BmgBI CACGTC 1 cut(s) 811
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 3 cut(s) 599, 851, 996
BmrFI CCNGG 6 cut(s) 167, 802, 1065, 1104, 1112, 2054
BmsI GCATC 6 cut(s) 330, 1291, 1313, 1467, 1693, 1866
BpiI GAAGAC 1 cut(s) 274
BpmI CTGGAG 3 cut(s) 98, 646, 884
Bpu10I CCTNAGC 1 cut(s) 1280
Bpu14I TTCGAA 4 cut(s) 527, 558, 613, 759
BpuMI CCSGG 2 cut(s) 167, 1112
Bsa29I ATCGAT 2 cut(s) 432, 795
BsaAI YACGTR 2 cut(s) 218, 1557
BsaHI GRCGYC 1 cut(s) 2049
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 1575
BsaWI WCCGGW 1 cut(s) 880
Bsc4I CCNNNNNNNGG 3 cut(s) 594, 965, 1314
Bse21I CCTNAGG 1 cut(s) 1512
Bse3DI GCAATG 2 cut(s) 158, 1134
BseBI CCWGG 4 cut(s) 802, 1065, 1104, 2054
BseCI ATCGAT 2 cut(s) 432, 795
BseDI CCNNGG 1 cut(s) 1575
BseGI GGATG 7 cut(s) 706, 1304, 1518, 1785, 1808, 1942, 2025
BseLI CCNNNNNNNGG 3 cut(s) 594, 965, 1314
BseMI GCAATG 2 cut(s) 158, 1134
BseMII CTCAG 4 cut(s) 704, 1165, 1271, 1503
BseRI GAGGAG 3 cut(s) 224, 707, 1263
BseSI GKGCMC 1 cut(s) 854
BseXI GCAGC 4 cut(s) 33, 1340, 1438, 1460
BseYI CCCAGC 1 cut(s) 1522
Bsh1236I CGCG 4 cut(s) 785, 1346, 1634, 1966
Bsh1285I CGRYCG 3 cut(s) 28, 913, 1098
BshFI GGCC 5 cut(s) 491, 1063, 1196, 1389, 1517
BshNI GGYRCC 2 cut(s) 849, 994
BshVI ATCGAT 2 cut(s) 432, 795
BsiEI CGRYCG 3 cut(s) 28, 913, 1098
BsiHKAI GWGCWC 1 cut(s) 504
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 5 cut(s) 31, 47, 166, 881, 1112
BsiWI CGTACG 1 cut(s) 906
BslI CCNNNNNNNGG 3 cut(s) 594, 965, 1314
BsmAI GTCTC 3 cut(s) 74, 99, 1491
BsmI GAATGC 3 cut(s) 227, 1324, 1791
BsnI GGCC 5 cut(s) 491, 1063, 1196, 1389, 1517
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 89
Bsp119I TTCGAA 4 cut(s) 527, 558, 613, 759
Bsp1286I GDGCHC 3 cut(s) 504, 576, 854
Bsp68I TCGCGA 1 cut(s) 1346
BspANI GGCC 5 cut(s) 491, 1063, 1196, 1389, 1517
BspCNI CTCAG 4 cut(s) 705, 1164, 1272, 1504
BspDI ATCGAT 2 cut(s) 432, 795
BspFNI CGCG 4 cut(s) 785, 1346, 1634, 1966
BspHI TCATGA 1 cut(s) 732
BspLI GGNNCC 3 cut(s) 599, 851, 996
BspMAI CTGCAG 2 cut(s) 125, 2032
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 4 cut(s) 777, 872, 1591, 1753
BspT104I TTCGAA 4 cut(s) 527, 558, 613, 759
BspT107I GGYRCC 2 cut(s) 849, 994
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 2 cut(s) 158, 1134
BssECI CCNNGG 1 cut(s) 1575
BssNI GRCGYC 1 cut(s) 2049
Bst2UI CCWGG 4 cut(s) 802, 1065, 1104, 2054
Bst4CI ACNGT 2 cut(s) 889, 2061
Bst6I CTCTTC 1 cut(s) 1477
BstACI GRCGYC 1 cut(s) 2049
BstBAI YACGTR 2 cut(s) 218, 1557
BstBI TTCGAA 4 cut(s) 527, 558, 613, 759
BstC8I GCNNGC 8 cut(s) 540, 1061, 1164, 1168, 1261, 1344, 1357, 1636
BstDEI CTNAG 5 cut(s) 496, 713, 1151, 1280, 1512
BstDSI CCRYGG 1 cut(s) 1575
BstF5I GGATG 7 cut(s) 706, 1304, 1518, 1785, 1808, 1942, 2025
BstFNI CGCG 4 cut(s) 785, 1346, 1634, 1966
BstH2I RGCGCY 1 cut(s) 1080
BstHHI GCGC 4 cut(s) 1079, 1448, 1968, 2040
BstMAI GTCTC 3 cut(s) 74, 99, 1491
BstMCI CGRYCG 3 cut(s) 28, 913, 1098
BstNI CCWGG 4 cut(s) 802, 1065, 1104, 2054
BstNSI RCATGY 1 cut(s) 952
BstSCI CCNGG 6 cut(s) 165, 800, 1063, 1102, 1110, 2052
BstSFI CTRYAG 4 cut(s) 121, 739, 894, 2028
BstSLI GKGCMC 1 cut(s) 854
BstUI CGCG 4 cut(s) 785, 1346, 1634, 1966
BstV1I GCAGC 4 cut(s) 33, 1340, 1438, 1460
BstV2I GAAGAC 1 cut(s) 274
Bsu15I ATCGAT 2 cut(s) 432, 795
Bsu36I CCTNAGG 1 cut(s) 1512
BsuI GTATCC 1 cut(s) 1277
BsuRI GGCC 5 cut(s) 491, 1063, 1196, 1389, 1517
BsuTUI ATCGAT 2 cut(s) 432, 795
BtgI CCRYGG 1 cut(s) 1575
BtgZI GCGATG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 811
BtsCI GGATG 7 cut(s) 706, 1304, 1518, 1785, 1808, 1942, 2025
BtsI GCAGTG 2 cut(s) 1646, 1841
BtsIMutI CAGTG 2 cut(s) 1646, 1841
BtuMI TCGCGA 1 cut(s) 1346
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 8 cut(s) 540, 1061, 1164, 1168, 1261, 1344, 1357, 1636
CciI TCATGA 1 cut(s) 732
CfoI GCGC 4 cut(s) 1079, 1448, 1968, 2040
Cfr13I GGNCC 1 cut(s) 598
ClaI ATCGAT 2 cut(s) 432, 795
CseI GACGC 3 cut(s) 157, 1640, 2038
CsiI ACCWGGT 1 cut(s) 1102
Csp6I GTAC 4 cut(s) 215, 907, 995, 2007
CviAII CATG 5 cut(s) 199, 733, 949, 1380, 1988
CviQI GTAC 4 cut(s) 215, 907, 995, 2007
DdeI CTNAG 5 cut(s) 496, 713, 1151, 1280, 1512
Eam1104I CTCTTC 1 cut(s) 1477
EarI CTCTTC 1 cut(s) 1477
EciI GGCGGA 1 cut(s) 1886
Ecl136II GAGCTC 1 cut(s) 502
Eco147I AGGCCT 1 cut(s) 1389
Eco24I GRGCYC 2 cut(s) 504, 576
Eco31I GGTCTC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 598
Eco53kI GAGCTC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 858
Eco81I CCTNAGG 1 cut(s) 1512
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 502
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 2 cut(s) 1000, 1291
EcoRII CCWGG 4 cut(s) 800, 1063, 1102, 2052
EcoT22I ATGCAT 2 cut(s) 1306, 1708
EcoT38I GRGCYC 2 cut(s) 504, 576
FaeI CATG 5 cut(s) 202, 736, 952, 1383, 1991
FalI AAGNNNNNCTT 4 cut(s) 479, 511, 1150, 1182
FatI CATG 5 cut(s) 198, 732, 948, 1379, 1987
FauI CCCGC 6 cut(s) 588, 790, 1473, 1553, 1627, 1859
FbaI TGATCA 5 cut(s) 975, 1251, 1539, 1687, 1782
Fnu4HI GCNGC 5 cut(s) 22, 1197, 1329, 1449, 1452
FokI GGATG 7 cut(s) 713, 1291, 1505, 1772, 1795, 1949, 2032
FriOI GRGCYC 2 cut(s) 504, 576
Fsp4HI GCNGC 5 cut(s) 22, 1197, 1329, 1449, 1452
FspBI CTAG 5 cut(s) 1217, 1608, 1652, 1914, 2004
GlaI GCGC 4 cut(s) 1078, 1447, 1967, 2039
GluI GCNGC 5 cut(s) 22, 1197, 1329, 1449, 1452
GsaI CCCAGC 1 cut(s) 1526
GsuI CTGGAG 3 cut(s) 98, 646, 884
HaeII RGCGCY 1 cut(s) 1080
HaeIII GGCC 5 cut(s) 491, 1063, 1196, 1389, 1517
HapII CCGG 5 cut(s) 31, 47, 166, 881, 1112
HgaI GACGC 3 cut(s) 157, 1640, 2038
HhaI GCGC 4 cut(s) 1079, 1448, 1968, 2040
Hin1I GRCGYC 1 cut(s) 2049
Hin1II CATG 5 cut(s) 202, 736, 952, 1383, 1991
Hin6I GCGC 4 cut(s) 1077, 1446, 1966, 2038
HinP1I GCGC 4 cut(s) 1077, 1446, 1966, 2038
HincII GTYRAC 1 cut(s) 1657
HindII GTYRAC 1 cut(s) 1657
HpaII CCGG 5 cut(s) 31, 47, 166, 881, 1112
HphI GGTGA 2 cut(s) 524, 895
Hpy166II GTNNAC 1 cut(s) 1657
Hpy8I GTNNAC 1 cut(s) 1657
Hpy99I CGWCG 5 cut(s) 151, 403, 812, 941, 944
HpyAV CCTTC 8 cut(s) 95, 317, 481, 620, 1008, 1062, 1152, 1541
HpyCH4III ACNGT 2 cut(s) 889, 2061
HpyCH4IV ACGT 5 cut(s) 41, 217, 810, 905, 1556
HpyF3I CTNAG 5 cut(s) 496, 713, 1151, 1280, 1512
HpySE526I ACGT 5 cut(s) 41, 217, 810, 905, 1556
Hsp92I GRCGYC 1 cut(s) 2049
Hsp92II CATG 5 cut(s) 202, 736, 952, 1383, 1991
HspAI GCGC 4 cut(s) 1077, 1446, 1966, 2038
KpnI GGTACC 1 cut(s) 998
Ksp22I TGATCA 5 cut(s) 975, 1251, 1539, 1687, 1782
LmnI GCTCC 5 cut(s) 507, 627, 865, 1534, 1973
Lsp1109I GCAGC 4 cut(s) 33, 1340, 1438, 1460
LweI GCATC 6 cut(s) 330, 1291, 1313, 1467, 1693, 1866
MabI ACCWGGT 1 cut(s) 1102
MaeI CTAG 5 cut(s) 1217, 1608, 1652, 1914, 2004
MaeII ACGT 5 cut(s) 41, 217, 810, 905, 1556
MaeIII GTNAC 3 cut(s) 512, 883, 1674
MboII GAAGA 8 cut(s) 185, 274, 671, 851, 1464, 1659, 1662, 1787
MfeI CAATTG 2 cut(s) 1323, 1778
MhlI GDGCHC 3 cut(s) 504, 576, 854
MlyI GAGTC 4 cut(s) 101, 361, 1493, 1911
Mph1103I ATGCAT 2 cut(s) 1306, 1708
MroXI GAANNNNTTC 2 cut(s) 174, 554
MseI TTAA 4 cut(s) 450, 618, 830, 1413
MslI CAYNNNNRTG 1 cut(s) 2014
MspA1I CMGCKG 3 cut(s) 1155, 1298, 1451
MspI CCGG 5 cut(s) 31, 47, 166, 881, 1112
MspR9I CCNGG 6 cut(s) 167, 802, 1065, 1104, 1112, 2054
MunI CAATTG 2 cut(s) 1323, 1778
Mva1269I GAATGC 3 cut(s) 227, 1324, 1791
MvaI CCWGG 4 cut(s) 802, 1065, 1104, 2054
MvnI CGCG 4 cut(s) 785, 1346, 1634, 1966
NciI CCSGG 2 cut(s) 167, 1112
NlaIII CATG 5 cut(s) 202, 736, 952, 1383, 1991
NlaIV GGNNCC 3 cut(s) 599, 851, 996
NmeAIII GCCGAG 1 cut(s) 1035
NmuCI GTSAC 3 cut(s) 512, 883, 1674
NruI TCGCGA 1 cut(s) 1346
NsiI ATGCAT 2 cut(s) 1306, 1708
NspI RCATGY 1 cut(s) 952
NspV TTCGAA 4 cut(s) 527, 558, 613, 759
PaeR7I CTCGAG 1 cut(s) 89
PagI TCATGA 1 cut(s) 732
PceI AGGCCT 1 cut(s) 1389
PcsI WCGNNNNNNNCGW 5 cut(s) 21, 184, 236, 438, 479
PctI GAATGC 3 cut(s) 227, 1324, 1791
PdmI GAANNNNTTC 2 cut(s) 174, 554
Pfl23II CGTACG 1 cut(s) 906
PfoI TCCNGGA 1 cut(s) 2052
PkrI GCNGC 5 cut(s) 23, 1198, 1330, 1450, 1453
Ple19I CGATCG 2 cut(s) 913, 1098
PleI GAGTC 4 cut(s) 100, 361, 1492, 1911
PpsI GAGTC 4 cut(s) 100, 361, 1492, 1911
Ppu21I YACGTR 2 cut(s) 218, 1557
PpuMI RGGWCCY 1 cut(s) 598
Psp124BI GAGCTC 1 cut(s) 504
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 4 cut(s) 800, 1063, 1102, 2052
PspFI CCCAGC 1 cut(s) 1522
PspGI CCWGG 4 cut(s) 800, 1063, 1102, 2052
PspLI CGTACG 1 cut(s) 906
PspN4I GGNNCC 3 cut(s) 599, 851, 996
PspPI GGNCC 1 cut(s) 598
PspPPI RGGWCCY 1 cut(s) 598
PstI CTGCAG 2 cut(s) 125, 2032
PvuI CGATCG 2 cut(s) 913, 1098
PvuII CAGCTG 1 cut(s) 1451
RruI TCGCGA 1 cut(s) 1346
RsaI GTAC 4 cut(s) 216, 908, 996, 2008
RsaNI GTAC 4 cut(s) 215, 907, 995, 2007
RseI CAYNNNNRTG 1 cut(s) 2014
SacI GAGCTC 1 cut(s) 504
SaqAI TTAA 4 cut(s) 450, 618, 830, 1413
SatI GCNGC 5 cut(s) 22, 1197, 1329, 1449, 1452
Sau96I GGNCC 1 cut(s) 598
SchI GAGTC 4 cut(s) 101, 361, 1493, 1911
ScrFI CCNGG 6 cut(s) 167, 802, 1065, 1104, 1112, 2054
SduI GDGCHC 3 cut(s) 504, 576, 854
SexAI ACCWGGT 1 cut(s) 1102
SfaNI GCATC 6 cut(s) 330, 1291, 1313, 1467, 1693, 1866
SfcI CTRYAG 4 cut(s) 121, 739, 894, 2028
Sfr274I CTCGAG 1 cut(s) 89
SfuI TTCGAA 4 cut(s) 527, 558, 613, 759
SinI GGWCC 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 2014
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
SpeI ACTAGT 1 cut(s) 2003
SseBI AGGCCT 1 cut(s) 1389
SspMI CTAG 5 cut(s) 1217, 1608, 1652, 1914, 2004
SstI GAGCTC 1 cut(s) 504
StuI AGGCCT 1 cut(s) 1389
StyD4I CCNGG 6 cut(s) 165, 800, 1063, 1102, 1110, 2052
TaaI ACNGT 2 cut(s) 889, 2061
TaiI ACGT 5 cut(s) 44, 220, 813, 908, 1559
TatI WGTACW 1 cut(s) 2006
TauI GCSGC 1 cut(s) 1199
Tru1I TTAA 4 cut(s) 450, 618, 830, 1413
Tru9I TTAA 4 cut(s) 450, 618, 830, 1413
TscAI CASTG 2 cut(s) 1653, 1848
TseFI GTSAC 3 cut(s) 512, 883, 1674
TseI GCWGC 4 cut(s) 21, 1328, 1448, 1451
Tsp45I GTSAC 3 cut(s) 512, 883, 1674
TspDTI ATGAA 5 cut(s) 17, 263, 459, 721, 1379
TspGWI ACGGA 5 cut(s) 656, 957, 1564, 1910, 1949
TspRI CASTG 2 cut(s) 1653, 1848
VpaK11BI GGWCC 1 cut(s) 598
XapI RAATTY 8 cut(s) 11, 65, 291, 650, 916, 1000, 1291, 1980
XceI RCATGY 1 cut(s) 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XhoI CTCGAG 1 cut(s) 89
XmnI GAANNNNTTC 2 cut(s) 174, 554
XspI CTAG 5 cut(s) 1217, 1608, 1652, 1914, 2004
Zsp2I ATGCAT 2 cut(s) 1306, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.