Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0003359

Physical Location & Seq

Genomic Coordinates Reverse (-)

63870316 .. 63872340

Gene Structure 2025 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00384530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGATCCAAAGATTAAACTCGGGTCGTCGGATCCTAACCTACCCGGCGGTTCATCCCTGCGAAGCCATAGCTCCGGAGACGCTTCTGATCTCACTCATCGCTCTCACCCACAACATTTGCTCTTACAAATCCAAATGCATCGCCAGCAACAAGCGAAACGCTTTGGAAGCAATCCGCCAAATCGGAGTGATTCGGGTTTTTTTGGAAGAGATCCAGGCCGGGCTGCCGGGTCTTCCGGACTCGACCGTTTTGAGCTTCTCGGAGCTCCATTTGGCTTTCCAGAAGGTTCAGTACTTGCTGGAAGACTGTACCCGGGACGACGCCCGGGTATGGATGCTGATGAATTCGGAGCGGGTTGCGAACCAGTTCCGGGTCTTGATTCGGGCGATGGCGACGGCTCTCGATGTTCTGCCTCTTGATGTCATCGACGTGGCAGTTGAGGTGAAGGAGCAGGTGGAATTGGTGATGAGGCAAGCGCGGCGGGTGAGGTTCGAAGTTGACCCGGAGGATAAACGGGTCGTCGAGGCGGTTAAGAAAGTTTTGATCGGGTTGGAGAAACGGGTCGACCCGGATAGGAGTGAGCTGAAGCGGGTACTTGATCATGTTGGGATTCGAAGATGGAGCAGGTGCGAGAAGGAGGTGAGATTTTTGGAATCTGAAATTGGGTTTGAGAATGTGAGAGAGGTGGGGTTGCTGAGCAGCCTGAAGGGGTTGTTGTGCTACTGCCGGTGCGTCATGTTCGGGTCTGTGGACGGGTTACCCGGGCCGCGACCCGAGCCCAGTAGCCGCAGCGAGGTGGTGAGTTGCCTGAATTTGGAAGATTTCCGGTGTCCGATATCGCTGGAGATAATGACGGATCCGGTGACGATCGGGACGGGTCACAGCTACGATCGGAGCTCCATTATGAAGTGGTTCAGAGCTGGAAAGCCGATTTGTCCGAAAACAGGGGAGAAAATGAAGAGCATGGAGGTGGTGCCTAATTTGGCATTGAAGAGGTTGATTCAGCAGTACTGCAATGAGAATGGGATTCCGGTTGGCGATTCGGGTCGTCGGAATCGCGACATTGGGAGGAGTACAGTTGGAGGTAGCTTGGCGGCTGAGGGAGCTATGAAAATGGTGGCGGAGTTTCTCTGTGCAAGTGTTGGGACTGGATCGAATAAGGCTGCTTATGAGATTCGGCTTCTTTCGAAAACAAGCATTTTTAACAGGTCTTGCTTGGTGGAAGCTGGAGTGGTGCCGCATTTGTTGAAGCTGTTGGCTTCCGCAGATTCGGAGACTCAAGAGAATGCGGTTGCAGGGTTGTTGAATCTGTCCAAGCATTCGAAAGGGAAAGCTGTGATTGTTGAAAATGGGGGTGTGGATTTGGTTGTGGATGTTTTGAAGGAGGGTCTCAAAGTTGAGGCTTGCCAGCATGCTGCTGGTACTTTGTTTTATCTTGCTTCCATTGAGGAGTACCGGATATTGATCGGAGAAAACCCGGGGGCCATACCGGCTTTGAGGGAGCTGATCAAGAACGATTCTGTTCGGGGCAAGAAGAATGCATTGGTTGCTATATTCGGCCTGCTTACACACCCTGCAAATCATAGAAGGGTGTTAGCAGCTGGGGTCGTTCCTTTGCTGTTTGAGATACTGACATCTTTTGACAGTGAAGTTCTTGTGACAGATTCTCTAGCGATTCTCGCAACTCTGGCTGAGAAACCCGAGGGTGCAGTTGCAATTTCGCGTTGCAAGGCTTTGGATTCCATTGTGGGGATTCTGAATTCTTCGACTTCAAGGGCGGCCAAGGAGTTCTGTGTGTCTTTGTTGCTGGCTCTTTGTGCAAATGGTGGCCACAATGTGGTTGCTCTTTTGGTTAAGAGCCCTTCTCTTATGGGATGTTTGTATTCCCAACTTAGTGAAGGCACATCTAGAGCAAGCAAAAGGGCCAGTGCTCTCATCAAGCTCCTCCATGAATTCCATGAAACAAGATCGTCTGGTCCACTCACTCAGGTTCTTCCACAGGACCGATTTGTTCACGTTAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009966 GO:0009987 GO:0010029 GO:0010119 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1900140 GO:1901419 GO:1901564 GO:1905957 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

674

Amino Acids

73.83

Weight (kDa)

8.66

Isoelectric Point (pI)

42.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	53 - 161	6.4e-14	PUB10-like, N-terminal domain
U-box	PF04564	273 - 341	5.4e-17	U-box domain
ARM_ARMC5	PF24768	364 - 550	6e-10	ARMC5
ARM_PUB	PF25598	386 - 649	2.3e-15	Plant U-box protein ARM repeat region
Arm	PF00514	402 - 438	2.7e-09	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10560 AT1G60190
fragaria_vesca	FvH4_4g34620 FvH4_5g05140
malus_domestica	MD06G1177100.v1.1 MD13G1090900.v1.1 MD14G1183300.v1.1 MD16G1091300.v1.1
prunus_persica	Prupe.1G255000_v2.0.a1 Prupe.5G175800_v2.0.a1
pyrus_communis	pycom06g15720 pycom13g07890 pycom14g15220 pycom16g07800
rosa_chinensis	RchiOBHm_Chr4g0443981 RchiOBHm_Chr7g0193831
rosa_laevigata	RLG00000004242 RLG00000005870
rosa_multiflora	Rmu_co8252843.1_g000001 Rmu_sc0001436.1_g000032 Rmu_sc0027702.1_g000002 Rmu_sc0029481.1_g000002
rosa_roxburghii	Rroxscaffold_3G00261790 Rroxscaffold_5G00384530
rosa_rugosa	Rorug04G0348700 Rorug07G0012400
rosa_samantha	Rh4AG409400 Rh4BG420500 Rh4CG434500 Rh4DG415500 Rh7AG139000 Rh7BG139200 Rh7CG142900 Rh7DG140500
rosa_wichuraiana	Rw4G035170 Rw7G011850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	442, 615
Acc36I	ACCTGC	2 cut(s)	442, 615
AccB1I	GGYRCC	2 cut(s)	973, 1234
AccB7I	CCANNNNNTGG	1 cut(s)	1837
AccBSI	CCGCTC	1 cut(s)	352
AccI	GTMKAC	1 cut(s)	564
AccII	CGCG	4 cut(s)	478, 769, 1059, 1723
AccIII	TCCGGA	2 cut(s)	73, 235
AclWI	GGATC	6 cut(s)	25, 38, 205, 851, 864, 1159
AcoI	YGGCCR	2 cut(s)	1779, 1828
AcsI	RAATTY	4 cut(s)	343, 811, 1759, 1952
AcuI	CTGAAG	2 cut(s)	605, 725
AcyI	GRCGYC	1 cut(s)	321
AdeI	CACNNNGTG	1 cut(s)	1837
AfaI	GTAC	7 cut(s)	293, 310, 594, 1010, 1075, 1423, 1454
AfiI	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 814, 944, 1749, 1837
AgsI	TTSAA	6 cut(s)	991, 1249, 1306, 1346, 1381, 1773
AjiI	CACGTC	1 cut(s)	430
AjnI	CCWGG	1 cut(s)	213
AjuI	GAANNNNNNNTTGG	4 cut(s)	645, 677, 1526, 1558
Alw21I	GWGCWC	3 cut(s)	267, 899, 1933
Alw26I	GTCTC	3 cut(s)	71, 1268, 1394
AlwI	GGATC	6 cut(s)	25, 38, 205, 851, 864, 1159
Ama87I	CYCGRG	7 cut(s)	19, 312, 324, 761, 773, 1477, 1700
Aor13HI	TCCGGA	2 cut(s)	73, 235
AoxI	GGCC	7 cut(s)	216, 764, 1482, 1558, 1779, 1828, 1923
ApeKI	GCWGC	6 cut(s)	223, 699, 789, 1163, 1415, 1598
ApoI	RAATTY	4 cut(s)	343, 811, 1759, 1952
Asp700I	GAANNNNTTC	2 cut(s)	365, 911
AspLEI	GCGC	1 cut(s)	478
AspS9I	GGNCC	5 cut(s)	764, 1482, 1923, 1976, 2002
AsuHPI	GGTGA	7 cut(s)	97, 454, 475, 496, 652, 811, 874
AsuII	TTCGAA	4 cut(s)	492, 613, 1187, 1322
AvaI	CYCGRG	7 cut(s)	19, 312, 324, 761, 773, 1477, 1700
AvaII	GGWCC	2 cut(s)	1976, 2002
BalI	TGGCCA	1 cut(s)	1830
BamHI	GGATCC	2 cut(s)	30, 856
BanI	GGYRCC	2 cut(s)	973, 1234
BanII	GRGCYC	4 cut(s)	267, 780, 899, 1862
BarI	GAAGNNNNNNTAC	2 cut(s)	275, 307
BbsI	GAAGAC	2 cut(s)	224, 309
Bbv12I	GWGCWC	3 cut(s)	267, 899, 1933
BbvCI	CCTCAGC	1 cut(s)	1098
BbvI	GCAGC	6 cut(s)	210, 711, 801, 1150, 1402, 1610
BccI	CCATC	2 cut(s)	382, 612
BceAI	ACGGC	1 cut(s)	411
BciT130I	CCWGG	1 cut(s)	215
BclI	TGATCA	2 cut(s)	598, 1506
BcoDI	GTCTC	3 cut(s)	71, 1268, 1394
BfaI	CTAG	2 cut(s)	1670, 1908
BfuAI	ACCTGC	2 cut(s)	442, 615
BglI	GCCNNNNNGGC	1 cut(s)	1490
BlpI	GCTNAGC	1 cut(s)	695
BmcAI	AGTACT	2 cut(s)	293, 1010
Bme18I	GGWCC	2 cut(s)	1976, 2002
BmeT110I	CYCGRG	7 cut(s)	19, 312, 324, 761, 773, 1477, 1700
BmgBI	CACGTC	1 cut(s)	430
BmgT120I	GGNCC	5 cut(s)	764, 1482, 1923, 1976, 2002
BmiI	GGNNCC	5 cut(s)	32, 858, 975, 1236, 1483
BmrI	ACTGGG	1 cut(s)	774
BmsI	GCATC	2 cut(s)	147, 324
BmuI	ACTGGG	1 cut(s)	774
BpiI	GAAGAC	2 cut(s)	224, 309
BplI	GAGNNNNNCTC	2 cut(s)	1849, 1881
BpmI	CTGGAG	2 cut(s)	863, 1248
Bpu10I	CCTNAGC	1 cut(s)	1098
Bpu1102I	GCTNAGC	1 cut(s)	695
Bpu14I	TTCGAA	4 cut(s)	492, 613, 1187, 1322
BpuEI	CTTGAG	1 cut(s)	1263
BsaBI	GATNNNNATC	1 cut(s)	1463
BsaHI	GRCGYC	1 cut(s)	321
BsaI	GGTCTC	1 cut(s)	1394
BsaJI	CCNNGG	7 cut(s)	312, 324, 761, 1477, 1478, 1701, 1782
BsaWI	WCCGGW	6 cut(s)	73, 235, 825, 859, 1030, 1455
Bsc4I	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 814, 944, 1749, 1837
Bse118I	RCCGGY	2 cut(s)	726, 1489
Bse1I	ACTGG	4 cut(s)	364, 780, 1153, 1926
Bse3DI	GCAATG	1 cut(s)	1021
Bse8I	GATNNNNATC	1 cut(s)	1463
BseAI	TCCGGA	2 cut(s)	73, 235
BseBI	CCWGG	1 cut(s)	215
BseDI	CCNNGG	7 cut(s)	312, 324, 761, 1477, 1478, 1701, 1782
BseGI	GGATG	4 cut(s)	52, 339, 1378, 1880
BseJI	GATNNNNATC	1 cut(s)	1463
BseLI	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 814, 944, 1749, 1837
BseMI	GCAATG	1 cut(s)	1021
BseMII	CTCAG	4 cut(s)	686, 1089, 1683, 2000
BseNI	ACTGG	4 cut(s)	364, 780, 1153, 1926
BseRI	GAGGAG	3 cut(s)	1084, 1463, 1934
BseXI	GCAGC	6 cut(s)	210, 711, 801, 1150, 1402, 1610
BseYI	CCCAGC	1 cut(s)	1601
BsgI	GTGCAG	1 cut(s)	1728
Bsh1236I	CGCG	4 cut(s)	478, 769, 1059, 1723
Bsh1285I	CGRYCG	3 cut(s)	246, 870, 892
BshFI	GGCC	7 cut(s)	218, 766, 1484, 1560, 1781, 1830, 1925
BshNI	GGYRCC	2 cut(s)	973, 1234
BsiEI	CGRYCG	3 cut(s)	246, 870, 892
BsiHKAI	GWGCWC	3 cut(s)	267, 899, 1933
BsiHKCI	CYCGRG	7 cut(s)	19, 312, 324, 761, 773, 1477, 1700
BslFI	GGGAC	3 cut(s)	329, 886, 1159
BslI	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 814, 944, 1749, 1837
BsmAI	GTCTC	3 cut(s)	71, 1268, 1394
BsmBI	CGTCTC	1 cut(s)	71
BsmFI	GGGAC	3 cut(s)	329, 886, 1159
BsmI	GAATGC	3 cut(s)	1291, 1318, 1543
BsnI	GGCC	7 cut(s)	218, 766, 1484, 1560, 1781, 1830, 1925
Bso31I	GGTCTC	1 cut(s)	1394
BsoBI	CYCGRG	7 cut(s)	19, 312, 324, 761, 773, 1477, 1700
Bsp119I	TTCGAA	4 cut(s)	492, 613, 1187, 1322
Bsp1286I	GDGCHC	5 cut(s)	267, 780, 899, 1862, 1933
Bsp13I	TCCGGA	2 cut(s)	73, 235
Bsp1720I	GCTNAGC	1 cut(s)	695
Bsp68I	TCGCGA	1 cut(s)	1059
BspANI	GGCC	7 cut(s)	218, 766, 1484, 1560, 1781, 1830, 1925
BspCNI	CTCAG	4 cut(s)	687, 1090, 1684, 1999
BspEI	TCCGGA	2 cut(s)	73, 235
BspFNI	CGCG	4 cut(s)	478, 769, 1059, 1723
BspLI	GGNNCC	5 cut(s)	32, 858, 975, 1236, 1483
BspMI	ACCTGC	2 cut(s)	442, 615
BspPI	GGATC	6 cut(s)	25, 38, 205, 851, 864, 1159
BspQI	GCTCTTC	1 cut(s)	953
BspT104I	TTCGAA	4 cut(s)	492, 613, 1187, 1322
BspT107I	GGYRCC	2 cut(s)	973, 1234
BspTNI	GGTCTC	1 cut(s)	1394
BsrBI	CCGCTC	1 cut(s)	352
BsrDI	GCAATG	1 cut(s)	1021
BsrFI	RCCGGY	2 cut(s)	726, 1489
BsrI	ACTGG	4 cut(s)	364, 780, 1153, 1926
BssAI	RCCGGY	2 cut(s)	726, 1489
BssECI	CCNNGG	7 cut(s)	312, 324, 761, 1477, 1478, 1701, 1782
BssNI	GRCGYC	1 cut(s)	321
BssT1I	CCWWGG	1 cut(s)	1782
Bst2UI	CCWGG	1 cut(s)	215
Bst4CI	ACNGT	4 cut(s)	247, 308, 1078, 1646
Bst6I	CTCTTC	3 cut(s)	201, 953, 986
BstACI	GRCGYC	1 cut(s)	321
BstAPI	GCANNNNNTGC	1 cut(s)	1547
BstBI	TTCGAA	4 cut(s)	492, 613, 1187, 1322
BstC8I	GCNNGC	8 cut(s)	145, 474, 1405, 1409, 1413, 1562, 1809, 1915
BstDEI	CTNAG	5 cut(s)	695, 1098, 1692, 1892, 1986
BstEII	GGTNACC	1 cut(s)	756
BstF5I	GGATG	4 cut(s)	52, 339, 1378, 1880
BstFNI	CGCG	4 cut(s)	478, 769, 1059, 1723
BstHHI	GCGC	1 cut(s)	478
BstMAI	GTCTC	3 cut(s)	71, 1268, 1394
BstMCI	CGRYCG	3 cut(s)	246, 870, 892
BstNI	CCWGG	1 cut(s)	215
BstNSI	RCATGY	1 cut(s)	1415
BstPI	GGTNACC	1 cut(s)	756
BstUI	CGCG	4 cut(s)	478, 769, 1059, 1723
BstV1I	GCAGC	6 cut(s)	210, 711, 801, 1150, 1402, 1610
BstV2I	GAAGAC	2 cut(s)	224, 309
BstX2I	RGATCY	3 cut(s)	30, 210, 856
BstYI	RGATCY	3 cut(s)	30, 210, 856
BsuRI	GGCC	7 cut(s)	218, 766, 1484, 1560, 1781, 1830, 1925
BtgZI	GCGATG	3 cut(s)	82, 124, 401
BtrI	CACGTC	1 cut(s)	430
BtsCI	GGATG	4 cut(s)	52, 339, 1378, 1880
BtsIMutI	CAGTG	2 cut(s)	1651, 1933
BtuMI	TCGCGA	1 cut(s)	1059
BveI	ACCTGC	2 cut(s)	442, 615
Cac8I	GCNNGC	8 cut(s)	145, 474, 1405, 1409, 1413, 1562, 1809, 1915
CfoI	GCGC	1 cut(s)	478
Cfr10I	RCCGGY	2 cut(s)	726, 1489
Cfr13I	GGNCC	5 cut(s)	764, 1482, 1923, 1976, 2002
Cfr9I	CCCGGG	4 cut(s)	312, 324, 761, 1477
CseI	GACGC	3 cut(s)	88, 329, 721
Csp6I	GTAC	7 cut(s)	292, 309, 593, 1009, 1074, 1422, 1453
CspCI	CAANNNNNGTGG	2 cut(s)	98, 133
CviAII	CATG	6 cut(s)	602, 736, 964, 1412, 1949, 1958
CviQI	GTAC	7 cut(s)	292, 309, 593, 1009, 1074, 1422, 1453
DdeI	CTNAG	5 cut(s)	695, 1098, 1692, 1892, 1986
DraIII	CACNNNGTG	1 cut(s)	1837
EaeI	YGGCCR	2 cut(s)	1779, 1828
Eam1104I	CTCTTC	3 cut(s)	201, 953, 986
EarI	CTCTTC	3 cut(s)	201, 953, 986
EciI	GGCGGA	2 cut(s)	164, 1136
Ecl136II	GAGCTC	2 cut(s)	265, 897
Eco130I	CCWWGG	1 cut(s)	1782
Eco24I	GRGCYC	4 cut(s)	267, 780, 899, 1862
Eco31I	GGTCTC	1 cut(s)	1394
Eco32I	GATATC	1 cut(s)	837
Eco47I	GGWCC	2 cut(s)	1976, 2002
Eco53kI	GAGCTC	2 cut(s)	265, 897
Eco57I	CTGAAG	2 cut(s)	605, 725
Eco88I	CYCGRG	7 cut(s)	19, 312, 324, 761, 773, 1477, 1700
Eco91I	GGTNACC	1 cut(s)	756
EcoICRI	GAGCTC	2 cut(s)	265, 897
EcoO65I	GGTNACC	1 cut(s)	756
EcoRI	GAATTC	3 cut(s)	343, 1759, 1952
EcoRII	CCWGG	1 cut(s)	213
EcoRV	GATATC	1 cut(s)	837
EcoT14I	CCWWGG	1 cut(s)	1782
EcoT22I	ATGCAT	2 cut(s)	140, 1543
EcoT38I	GRGCYC	4 cut(s)	267, 780, 899, 1862
ErhI	CCWWGG	1 cut(s)	1782
Esp3I	CGTCTC	1 cut(s)	71
FaeI	CATG	6 cut(s)	605, 739, 967, 1415, 1952, 1961
FaqI	GGGAC	3 cut(s)	329, 886, 1159
FatI	CATG	6 cut(s)	601, 735, 963, 1411, 1948, 1957
FauI	CCCGC	3 cut(s)	345, 474, 582
FbaI	TGATCA	2 cut(s)	598, 1506
FblI	GTMKAC	1 cut(s)	564
FokI	GGATG	4 cut(s)	39, 346, 1385, 1887
FriOI	GRGCYC	4 cut(s)	267, 780, 899, 1862
FspBI	CTAG	2 cut(s)	1670, 1908
GlaI	GCGC	1 cut(s)	477
GsaI	CCCAGC	1 cut(s)	1605
GsuI	CTGGAG	2 cut(s)	863, 1248
HaeIII	GGCC	7 cut(s)	218, 766, 1484, 1560, 1781, 1830, 1925
HgaI	GACGC	3 cut(s)	88, 329, 721
HhaI	GCGC	1 cut(s)	478
Hin1I	GRCGYC	1 cut(s)	321
Hin1II	CATG	6 cut(s)	605, 739, 967, 1415, 1952, 1961
Hin6I	GCGC	1 cut(s)	476
HinP1I	GCGC	1 cut(s)	476
HincII	GTYRAC	2 cut(s)	499, 565
HindII	GTYRAC	2 cut(s)	499, 565
HphI	GGTGA	7 cut(s)	97, 454, 475, 496, 652, 811, 874
Hpy166II	GTNNAC	5 cut(s)	499, 565, 751, 1979, 2014
Hpy8I	GTNNAC	5 cut(s)	499, 565, 751, 1979, 2014
Hpy99I	CGWCG	6 cut(s)	30, 323, 397, 431, 524, 1053
HpyAV	CCTTC	8 cut(s)	277, 439, 628, 700, 1375, 1581, 1872, 1892
HpyCH4III	ACNGT	4 cut(s)	247, 308, 1078, 1646
HpyCH4IV	ACGT	2 cut(s)	429, 2016
HpyF3I	CTNAG	5 cut(s)	695, 1098, 1692, 1892, 1986
HpySE526I	ACGT	2 cut(s)	429, 2016
Hsp92I	GRCGYC	1 cut(s)	321
Hsp92II	CATG	6 cut(s)	605, 739, 967, 1415, 1952, 1961
HspAI	GCGC	1 cut(s)	476
Kpn2I	TCCGGA	2 cut(s)	73, 235
Ksp22I	TGATCA	2 cut(s)	598, 1506
LguI	GCTCTTC	1 cut(s)	953
Lsp1109I	GCAGC	6 cut(s)	210, 711, 801, 1150, 1402, 1610
LweI	GCATC	2 cut(s)	147, 324
MaeI	CTAG	2 cut(s)	1670, 1908
MaeII	ACGT	2 cut(s)	429, 2016
MaeIII	GTNAC	4 cut(s)	756, 862, 878, 1657
MbiI	CCGCTC	1 cut(s)	352
MflI	RGATCY	3 cut(s)	30, 210, 856
MhlI	GDGCHC	5 cut(s)	267, 780, 899, 1862, 1933
MlsI	TGGCCA	1 cut(s)	1830
MluCI	AATT	8 cut(s)	343, 458, 660, 811, 979, 1716, 1759, 1952
MluNI	TGGCCA	1 cut(s)	1830
MlyI	GAGTC	2 cut(s)	233, 1270
MmeI	TCCRAC	4 cut(s)	8, 531, 1031, 1060
Mox20I	TGGCCA	1 cut(s)	1830
Mph1103I	ATGCAT	2 cut(s)	140, 1543
MroI	TCCGGA	2 cut(s)	73, 235
MroXI	GAANNNNTTC	2 cut(s)	365, 911
MscI	TGGCCA	1 cut(s)	1830
MseI	TTAA	4 cut(s)	14, 531, 1203, 1854
MslI	CAYNNNNRTG	2 cut(s)	428, 968
Msp20I	TGGCCA	1 cut(s)	1830
MspA1I	CMGCKG	1 cut(s)	1601
Mva1269I	GAATGC	3 cut(s)	1291, 1318, 1543
MvaI	CCWGG	1 cut(s)	215
MvnI	CGCG	4 cut(s)	478, 769, 1059, 1723
NlaIII	CATG	6 cut(s)	605, 739, 967, 1415, 1952, 1961
NlaIV	GGNNCC	5 cut(s)	32, 858, 975, 1236, 1483
NmuCI	GTSAC	3 cut(s)	862, 878, 1657
NruI	TCGCGA	1 cut(s)	1059
NsiI	ATGCAT	2 cut(s)	140, 1543
NspI	RCATGY	1 cut(s)	1415
NspV	TTCGAA	4 cut(s)	492, 613, 1187, 1322
PaeI	GCATGC	1 cut(s)	1415
PaqCI	CACCTGC	2 cut(s)	442, 615
PciSI	GCTCTTC	1 cut(s)	953
PctI	GAATGC	3 cut(s)	1291, 1318, 1543
PdmI	GAANNNNTTC	2 cut(s)	365, 911
PflMI	CCANNNNNTGG	1 cut(s)	1837
Ple19I	CGATCG	2 cut(s)	870, 892
PleI	GAGTC	2 cut(s)	233, 1270
PpsI	GAGTC	2 cut(s)	233, 1270
Psp124BI	GAGCTC	2 cut(s)	267, 899
Psp6I	CCWGG	1 cut(s)	213
PspEI	GGTNACC	1 cut(s)	756
PspFI	CCCAGC	1 cut(s)	1601
PspGI	CCWGG	1 cut(s)	213
PspN4I	GGNNCC	5 cut(s)	32, 858, 975, 1236, 1483
PspPI	GGNCC	5 cut(s)	764, 1482, 1923, 1976, 2002
PsuI	RGATCY	3 cut(s)	30, 210, 856
PvuI	CGATCG	2 cut(s)	870, 892
PvuII	CAGCTG	1 cut(s)	1601
RruI	TCGCGA	1 cut(s)	1059
RsaI	GTAC	7 cut(s)	293, 310, 594, 1010, 1075, 1423, 1454
RsaNI	GTAC	7 cut(s)	292, 309, 593, 1009, 1074, 1422, 1453
RseI	CAYNNNNRTG	2 cut(s)	428, 968
SacI	GAGCTC	2 cut(s)	267, 899
SalI	GTCGAC	1 cut(s)	563
SapI	GCTCTTC	1 cut(s)	953
SaqAI	TTAA	4 cut(s)	14, 531, 1203, 1854
Sau96I	GGNCC	5 cut(s)	764, 1482, 1923, 1976, 2002
ScaI	AGTACT	2 cut(s)	293, 1010
SchI	GAGTC	2 cut(s)	233, 1270
SduI	GDGCHC	5 cut(s)	267, 780, 899, 1862, 1933
SfaNI	GCATC	2 cut(s)	147, 324
SfuI	TTCGAA	4 cut(s)	492, 613, 1187, 1322
SinI	GGWCC	2 cut(s)	1976, 2002
SmaI	CCCGGG	4 cut(s)	314, 326, 763, 1479
SmiMI	CAYNNNNRTG	2 cut(s)	428, 968
SmlI	CTYRAG	1 cut(s)	1278
SmoI	CTYRAG	1 cut(s)	1278
SphI	GCATGC	1 cut(s)	1415
Sse9I	AATT	8 cut(s)	343, 458, 660, 811, 979, 1716, 1759, 1952
SspMI	CTAG	2 cut(s)	1670, 1908
SstI	GAGCTC	2 cut(s)	267, 899
StyI	CCWWGG	1 cut(s)	1782
TaaI	ACNGT	4 cut(s)	247, 308, 1078, 1646
TaiI	ACGT	2 cut(s)	432, 2019
TaqII	GACCGA	1 cut(s)	2019
TasI	AATT	8 cut(s)	343, 458, 660, 811, 979, 1716, 1759, 1952
TatI	WGTACW	3 cut(s)	291, 1008, 1073
TauI	GCSGC	6 cut(s)	481, 769, 789, 1097, 1240, 1781
Tru1I	TTAA	4 cut(s)	14, 531, 1203, 1854
Tru9I	TTAA	4 cut(s)	14, 531, 1203, 1854
TscAI	CASTG	2 cut(s)	1651, 1933
TseFI	GTSAC	3 cut(s)	862, 878, 1657
TseI	GCWGC	6 cut(s)	223, 699, 789, 1163, 1415, 1598
Tsp45I	GTSAC	3 cut(s)	862, 878, 1657
TspDTI	ATGAA	7 cut(s)	41, 356, 920, 971, 1124, 1965, 1974
TspGWI	ACGGA	1 cut(s)	869
TspMI	CCCGGG	4 cut(s)	312, 324, 761, 1477
TspRI	CASTG	2 cut(s)	1651, 1933
Van91I	CCANNNNNTGG	1 cut(s)	1837
VpaK11BI	GGWCC	2 cut(s)	1976, 2002
XapI	RAATTY	4 cut(s)	343, 811, 1759, 1952
XbaI	TCTAGA	1 cut(s)	1907
XceI	RCATGY	1 cut(s)	1415
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1415
XmaI	CCCGGG	4 cut(s)	312, 324, 761, 1477
XmiI	GTMKAC	1 cut(s)	564
XmnI	GAANNNNTTC	2 cut(s)	365, 911
XspI	CTAG	2 cut(s)	1670, 1908
ZrmI	AGTACT	2 cut(s)	293, 1010
Zsp2I	ATGCAT	2 cut(s)	140, 1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.