Rorug07G0012400

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003359
Physical Location & Seq
Reverse (-)
980812 .. 986634
5823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0012400.1

Sequence Viewer

Length: 3357 bp
ATGGCGATTGTGACCGGAGATCGGTACCTGGAGAAGCTAGTCCACTTCGTGGAGCAGCAAGCCGGTTCTCTGATCGACGGTTCGTTAGTGCTGAAGCTCAACCCGGCCGGCTTCCACTACGTCCACTCCCGCCTCGAGGCCTTGCACGAGCTCGAGAGCCTCCTCGCCGGCGCTCCCGTCGACTATCTCCGAGCCTATATCTCCGACCTCGGCGACCACCGCGCCCTCGAGCAGCTCAGGAGGATTCTCAGGCTGTTGACGTCGCTTAAGGTCGTTTCGGTGCTGCCGGCTCCTGCCAGAGACCCCACGCCGCTGTCGTTTTGGCCGTTCGGGAGGCTTAGGGTTTTGGAGCTTCGGGGATGCGATTTGTCCACGTCGGCCGCGAAAGGTTTGCTCGAGTTGAGGCACACCTTGGAGAAGATCATCTGTCACAATTCAACTGATGCCCTGCGCCATGTGTTTGCGAGTAGAATTGCAGAGATAAGGGACTCGCCACTGTGGCACCGGTTGTCATTTGTTTCGTGTGCTTGTAATGGCTTGGTTCTGATGGATGAGTCTTTGCAACTACTTCCGGCAGTTGAAACTCTTGACCTGAGCCGAAACAAGTTTGCAAAGATTGACAATCTTCGCAAGTGTGGCAAATTGAAACACCTAGACCTTGGTTTCAATCATCTGAGAACGATTTCGTCATTTGGTGAGGTCACCTCTCGACTACTTAAACTTGTTTTGAGGAACAATGCTCTAACTACGTTGCGTGGGATTGAGACTTTGAAGTCCCTTGAAGGGCTTGATGTTTCCTACAATATTATTTCCAATTTTTCGGAGTTAGAGTTCCTTGGTGGTCTTCCATCTCTACAAAGCTTGTGGCTGGAAGGGAATCCATTATGTTGTGCCAGTTGGTATCGGCCACAAGTATTCAGCTATTTCTATAATCCAGAAAAATTGAAGTTAGATGATAAGGAAATCAGCACCAGAGAGTTTTGGAAGAGGCAACTAATTATTGCAAGTAGACAGAAGCGACCTGCCAGCTTTGGGTTTTATTCTCCCATAAAGTGTGATGAGAAAGGAGATGCAAGTATCAACAGAAGAAGGAAAAAGGCGTCTCGTCTTGCTTCCATTGTAAGTGAAGAAGGGAGCACATCTCTCTGTTCTGATCAGGAGTCTGTGTCTTGTGACAATGAGATTCAAAGCAGAGAAGAACTTGTTATATCAGATGAGGAAGCTGAAATTGTTGATTTGATGACTAGAGTTGAGCAAATGAAGAAAGAGCGCTCTGTTCTTTGGTTGCGAGAGTTCAAAGAATGGTTGGATCATGCTCCTGAGGATTCTGTGGACAGCAATAGACATAGCGGGACGACATTGCATATTGGCAGGGAAAATTATATAAAAGAAAAGTCAAGCTGGAGGCAGCTTGGTGAAAACTCCAGATTTGTTTCAGACTATGCTCAAGCATCAGGAGATGAGAGTAGTACAAATGTTTTAGATTCAGATAGTTCATTTATGGATATGTCTACTGGTTTGCATGCCCATCACTTTGATCAAACTGGTTCCTTGGGAAATGCTGGTTTTGTCATCCCAGTTGGCATGGATAGAAGAGATCTGAAGGAAACCCTAAAAGATAGTTCACTAGAAGGAACAAGTAGTGTCCCTTTACGAGCAAAAAGTTTCCATGGTCACATATTCACCGGTCAAAGAGGTCATAGGATGGTCGAAAATTTGAGTATGTCATTGACTTCTATAGATGATATTTCTGAATCTCGCTCCTCGTCTGTGTGCCCGGGATCACCACCTCATTATCAAAAGGACATTCTCAACCGTCGTCATAACTTGGAGGAAGAAATTTTACAGTTATCTGCAGAGTCATTTTCAGTGGCATCATCTGATAGTAATACCAGCTGTAGTGAAGACGATAACTATGAGTCTAGGCAGTCCATTCCTGAAGGTCATCAGCTACTGAATAAAAATATGGAAGAACATCCGTCTTCAGATCCTTTTAGGTTGTATGACATGAGATATGAGGTTCCACATGTAAGAGGAAATGACAAGTGTTCAGTTGGAATAGGGGCAGAGAAAATTTCAAATTCAGACCTATCGCTGCAATCACATGCCAGTGATGTCCCTGGTCGTGCTCATCATGGTGAGATTGCTCATTTTGTTGATGAAGAAGGTGATTTGTTAGAGAGGAGAAAACGCAGACAGAAAATAAAAAGAAGAGTTGTTACACTACTAGAAGATGACAAAATGGTTAGGCAAGCAGAAACATTACCAACATTAAATGGTAATATAGAGAATCATGTAACAATACTGGAAGATGAGCAAGATAGTCGATCTTTCTATGGGATTAACCTTGATGAAGTTATTGGTAAGAACCAGATGTTGACAAATACAAGTAATATCCCACTAATAAATGATACTACTGGATCTTCTGGGGCTGAATGTTTCTCTTCAAAGAGTGATGAATTTATTGAGGACTATTTCAGAAAGAATGTTGCAGATATGGGAAATAATGAAATTTGCAAGCAGTATATGCGCTGCTATTGTATACTTGAGTTGGATTCTCTATACAGAGAAAGAGAAGTAGCTGTCTTGCTGAGCAGTGAAAACAAAGTGTATGTGCTACTAATTGGCATTGGAGGTGATGGATCAGGCACCATCTTGAATTTGCAGGGTTCTCATAGGGTTGAAGACATAAGAGACGTCGTAGTTGGTGTAGGACTTCAGGTTGTGAGGGTGTATGTCGAGGAGAGTGCTACCTACCTCTTTAAAACCAGAAGCATTGAAAGTTCAAGACAGTTACTTTCAACTTTAACAGTTATCGATTCTTATTCTCCAATCGATAAATTTTGTTTGAGGAGTTTGGAGCAGGTTCAGGTTGGACTATTTGAGAAACAAATATGTGGAGGTTCAAAACTGAGCATATTCCAGTATTCTATGGTGCAGTTTTGGTGTAGTAGTAGTGAAGATGGAGCATGGCTTTCAAGATCAATATTTGTGGCTGGAGAGCATTTATTTGTGTGCTTTGAAGACCTCATGCAGTTTAGCTCTTTATCAGAGGATACGTCTCTACCTCCATATTTTTCACTTGACTTGTGTTGCTCTATTGCTGACATATCTGAGCTGGTTGTCGAGACCAGGGAAAATCGGTGTGTAACCTTAGCTGTTGAATGTGCAATGTCGGAATTCTGTTCTTCACCCAAAGCTGGGAAACAAGACCCCGGTGAAAATGACATAAAGACAGCTTCAGGCTCTATGACTTGGAAACTCAAGTGGTTTTCTGAAGAGAGTCCGTTCAAGTTTGTGGCATTGTTGAAGGCAATCCATGCCGGGTTGACTATTTCTCCTTTGCTGATAAGATGA

Protein Analysis

1118

Amino Acids

125.76

Weight (kDa)

5.55

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 192 - 231 3.5e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 772
AatII GACGTC 2 cut(s) 263, 2700
AbsI CCTCGAGG 1 cut(s) 134
Acc36I ACCTGC 2 cut(s) 1030, 2854
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 3 cut(s) 24, 501, 2648
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 4 cut(s) 180, 1009, 1511, 2542
AccII CGCG 2 cut(s) 222, 383
AciI CCGC 5 cut(s) 130, 220, 311, 381, 1350
AclWI GGATC 5 cut(s) 1317, 1789, 1982, 2428, 2650
AcoI YGGCCR 4 cut(s) 105, 323, 378, 905
AcsI RAATTY 9 cut(s) 1714, 1839, 2073, 2080, 2459, 2511, 2659, 2840, 3179
AcuI CTGAAG 7 cut(s) 113, 1622, 1959, 1968, 2702, 3225, 3297
AcyI GRCGYC 3 cut(s) 260, 1100, 2697
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 2 cut(s) 26, 1471
AfeI AGCGCT 1 cut(s) 1271
AfiI CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3200, 3201
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 2026
AgeI ACCGGT 2 cut(s) 504, 1685
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 3 cut(s) 27, 2119, 3131
AloI GAACNNNNNNTCC 2 cut(s) 815, 847
Alw21I GWGCWC 3 cut(s) 153, 1139, 2131
Alw26I GTCTC 6 cut(s) 294, 758, 1107, 2688, 3066, 3122
AlwI GGATC 5 cut(s) 1317, 1789, 1982, 2428, 2650
AlwNI CAGNNNCTG 1 cut(s) 1954
Ama87I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Aor51HI AGCGCT 1 cut(s) 1271
AoxI GGCC 5 cut(s) 105, 138, 323, 378, 905
ApeKI GCWGC 6 cut(s) 55, 232, 283, 1408, 2095, 2532
ApoI RAATTY 9 cut(s) 1714, 1839, 2073, 2080, 2459, 2511, 2659, 2840, 3179
AsiGI ACCGGT 2 cut(s) 504, 1685
Asp700I GAANNNNTTC 1 cut(s) 682
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 5 cut(s) 173, 224, 453, 1272, 2532
AsuC2I CCSGG 5 cut(s) 104, 1778, 1779, 3216, 3325
AvaI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
AxyI CCTNAGG 1 cut(s) 1320
BaeGI GKGCMC 1 cut(s) 1778
BanI GGYRCC 3 cut(s) 24, 501, 2648
BanII GRGCYC 1 cut(s) 153
BarI GAAGNNNNNNTAC 4 cut(s) 1827, 1859, 2203, 2235
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 5 cut(s) 836, 1911, 1974, 2691, 3030
Bbv12I GWGCWC 3 cut(s) 153, 1139, 2131
BbvI GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2519
BccI CCATC 7 cut(s) 541, 856, 1536, 1699, 2633, 2660, 2957
BceAI ACGGC 1 cut(s) 310
BcgI CGANNNNNNTGC 6 cut(s) 373, 407, 2306, 2340, 2729, 2763
BciT130I CCWGG 3 cut(s) 29, 2121, 3133
BciVI GTATCC 1 cut(s) 3049
BclI TGATCA 2 cut(s) 1153, 1537
BcnI CCSGG 5 cut(s) 104, 1778, 1779, 3216, 3325
BcoDI GTCTC 6 cut(s) 294, 758, 1107, 2688, 3066, 3122
BfaI CTAG 6 cut(s) 38, 653, 1245, 1628, 1923, 2228
BfmI CTRYAG 3 cut(s) 1737, 1854, 1897
BfoI RGCGCY 2 cut(s) 174, 1273
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 2 cut(s) 1030, 2854
BfuI GTATCC 1 cut(s) 3049
BglI GCCNNNNNGGC 1 cut(s) 499
BglII AGATCT 1 cut(s) 1597
BisI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2533
BlpI GCTNAGC 1 cut(s) 2591
BlsI GCNGC 8 cut(s) 57, 234, 285, 312, 382, 1410, 2097, 2534
Bme1390I CCNGG 8 cut(s) 29, 104, 1778, 1779, 2121, 3133, 3216, 3325
BmeT110I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
BmgBI CACGTC 1 cut(s) 375
BmiI GGNNCC 6 cut(s) 26, 291, 503, 1549, 2022, 2650
BmrFI CCNGG 8 cut(s) 29, 104, 1778, 1779, 2121, 3133, 3216, 3325
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
BmuI ACTGGG 1 cut(s) 1571
BpiI GAAGAC 5 cut(s) 836, 1911, 1974, 2691, 3030
BplI GAGNNNNNCTC 4 cut(s) 689, 721, 1126, 1158
BpmI CTGGAG 4 cut(s) 50, 1408, 1423, 3018
Bpu10I CCTNAGC 4 cut(s) 236, 338, 593, 3154
Bpu1102I GCTNAGC 1 cut(s) 2591
BpuEI CTTGAG 3 cut(s) 1431, 2567, 3248
BpuMI CCSGG 5 cut(s) 104, 1778, 1779, 3216, 3325
Bsa29I ATCGAT 2 cut(s) 2817, 2835
BsaHI GRCGYC 3 cut(s) 260, 1100, 2697
BsaI GGTCTC 2 cut(s) 294, 3122
BsaWI WCCGGW 3 cut(s) 14, 504, 1685
BsaXI ACNNNNNCTCC 8 cut(s) 110, 140, 815, 845, 1151, 1181, 3322, 3352
Bsc4I CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3200, 3201
Bse118I RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
Bse1I ACTGG 8 cut(s) 894, 1519, 1549, 1577, 2109, 2310, 2422, 2923
Bse21I CCTNAGG 1 cut(s) 1320
Bse3DI GCAATG 2 cut(s) 1358, 3177
BseBI CCWGG 3 cut(s) 29, 2121, 3133
BseCI ATCGAT 2 cut(s) 2817, 2835
BseGI GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BseLI CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3200, 3201
BseMI GCAATG 2 cut(s) 1358, 3177
BseMII CTCAG 8 cut(s) 250, 262, 584, 665, 1311, 2582, 2903, 3105
BseNI ACTGG 8 cut(s) 894, 1519, 1549, 1577, 2109, 2310, 2422, 2923
BseRI GAGGAG 5 cut(s) 152, 1753, 2197, 2756, 2866
BseSI GKGCMC 1 cut(s) 1778
BseX3I CGGCCG 2 cut(s) 105, 378
BseXI GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2519
BseYI CCCAGC 1 cut(s) 3200
BsgI GTGCAG 1 cut(s) 2957
Bsh1236I CGCG 2 cut(s) 222, 383
Bsh1285I CGRYCG 2 cut(s) 108, 381
BshFI GGCC 5 cut(s) 107, 140, 325, 380, 907
BshNI GGYRCC 3 cut(s) 24, 501, 2648
BshTI ACCGGT 2 cut(s) 504, 1685
BshVI ATCGAT 2 cut(s) 2817, 2835
BsiEI CGRYCG 2 cut(s) 108, 381
BsiHKAI GWGCWC 3 cut(s) 153, 1139, 2131
BsiHKCI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
BslFI GGGAC 5 cut(s) 500, 760, 1366, 1631, 2102
BslI CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3200, 3201
BsmAI GTCTC 6 cut(s) 294, 758, 1107, 2688, 3066, 3122
BsmBI CGTCTC 3 cut(s) 1107, 2688, 3066
BsmFI GGGAC 5 cut(s) 500, 760, 1366, 1631, 2102
BsnI GGCC 5 cut(s) 107, 140, 325, 380, 907
Bso31I GGTCTC 2 cut(s) 294, 3122
BsoBI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Bsp1286I GDGCHC 4 cut(s) 153, 1139, 1778, 2131
Bsp1720I GCTNAGC 1 cut(s) 2591
Bsp19I CCATGG 1 cut(s) 1669
BspACI CCGC 5 cut(s) 130, 220, 311, 381, 1350
BspANI GGCC 5 cut(s) 107, 140, 325, 380, 907
BspCNI CTCAG 8 cut(s) 249, 261, 585, 666, 1312, 2583, 2904, 3106
BspDI ATCGAT 2 cut(s) 2817, 2835
BspFNI CGCG 2 cut(s) 222, 383
BspLI GGNNCC 6 cut(s) 26, 291, 503, 1549, 2022, 2650
BspMAI CTGCAG 1 cut(s) 1858
BspMI ACCTGC 2 cut(s) 1030, 2854
BspPI GGATC 5 cut(s) 1317, 1789, 1982, 2428, 2650
BspT107I GGYRCC 3 cut(s) 24, 501, 2648
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 2 cut(s) 294, 3122
BsrDI GCAATG 2 cut(s) 1358, 3177
BsrFI RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
BsrI ACTGG 8 cut(s) 894, 1519, 1549, 1577, 2109, 2310, 2422, 2923
BssAI RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
BssNAI GTATAC 1 cut(s) 2543
BssNI GRCGYC 3 cut(s) 260, 1100, 2697
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
Bst1107I GTATAC 1 cut(s) 2543
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 3 cut(s) 29, 2121, 3133
Bst4CI ACNGT 6 cut(s) 80, 498, 1817, 1848, 2793, 2812
Bst6I CTCTTC 5 cut(s) 980, 1588, 2206, 2449, 3273
BstACI GRCGYC 3 cut(s) 260, 1100, 2697
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 2 cut(s) 2527, 3320
BstC8I GCNNGC 8 cut(s) 60, 109, 169, 288, 1027, 1524, 2253, 2519
BstDSI CCRYGG 1 cut(s) 1669
BstEII GGTNACC 1 cut(s) 700
BstF5I GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BstFNI CGCG 2 cut(s) 222, 383
BstH2I RGCGCY 2 cut(s) 174, 1273
BstHHI GCGC 5 cut(s) 173, 224, 453, 1272, 2532
BstMAI GTCTC 6 cut(s) 294, 758, 1107, 2688, 3066, 3122
BstMCI CGRYCG 2 cut(s) 108, 381
BstMWI GCNNNNNNNGC 5 cut(s) 219, 499, 636, 2527, 3320
BstNI CCWGG 3 cut(s) 29, 2121, 3133
BstNSI RCATGY 3 cut(s) 1526, 2030, 2108
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 8 cut(s) 27, 102, 1776, 1777, 2119, 3131, 3214, 3323
BstSFI CTRYAG 3 cut(s) 1737, 1854, 1897
BstSLI GKGCMC 1 cut(s) 1778
BstUI CGCG 2 cut(s) 222, 383
BstV1I GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2519
BstV2I GAAGAC 5 cut(s) 836, 1911, 1974, 2691, 3030
BstX2I RGATCY 3 cut(s) 1597, 1987, 2420
BstYI RGATCY 3 cut(s) 1597, 1987, 2420
BstZ17I GTATAC 1 cut(s) 2543
BstZI CGGCCG 2 cut(s) 105, 378
Bsu15I ATCGAT 2 cut(s) 2817, 2835
Bsu36I CCTNAGG 1 cut(s) 1320
BsuI GTATCC 1 cut(s) 3049
BsuRI GGCC 5 cut(s) 107, 140, 325, 380, 907
BsuTUI ATCGAT 2 cut(s) 2817, 2835
BtgI CCRYGG 1 cut(s) 1669
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BtsI GCAGTG 1 cut(s) 2602
BtsIMutI CAGTG 4 cut(s) 494, 1875, 2116, 2602
BveI ACCTGC 2 cut(s) 1030, 2854
Cac8I GCNNGC 8 cut(s) 60, 109, 169, 288, 1027, 1524, 2253, 2519
CaiI CAGNNNCTG 1 cut(s) 1954
CfoI GCGC 5 cut(s) 173, 224, 453, 1272, 2532
Cfr10I RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
Cfr9I CCCGGG 1 cut(s) 1777
ClaI ATCGAT 2 cut(s) 2817, 2835
CseI GACGC 1 cut(s) 1089
Csp6I GTAC 2 cut(s) 25, 1470
CspAI ACCGGT 2 cut(s) 504, 1685
CspCI CAANNNNNGTGG 4 cut(s) 845, 880, 2973, 3008
CviQI GTAC 2 cut(s) 25, 1470
DraI TTTAAA 1 cut(s) 2764
DraIII CACNNNGTG 1 cut(s) 49
DrdI GACNNNNNNGTC 1 cut(s) 772
DseDI GACNNNNNNGTC 1 cut(s) 772
EaeI YGGCCR 4 cut(s) 105, 323, 378, 905
EagI CGGCCG 2 cut(s) 105, 378
Eam1104I CTCTTC 5 cut(s) 980, 1588, 2206, 2449, 3273
EarI CTCTTC 5 cut(s) 980, 1588, 2206, 2449, 3273
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 2 cut(s) 105, 378
Eco130I CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 2 cut(s) 294, 3122
Eco47III AGCGCT 1 cut(s) 1271
Eco52I CGGCCG 2 cut(s) 105, 378
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 7 cut(s) 113, 1622, 1959, 1968, 2702, 3225, 3297
Eco81I CCTNAGG 1 cut(s) 1320
Eco88I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Eco91I GGTNACC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 700
EcoRI GAATTC 1 cut(s) 3179
EcoRII CCWGG 3 cut(s) 27, 2119, 3131
EcoT14I CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
Esp3I CGTCTC 3 cut(s) 1107, 2688, 3066
FaqI GGGAC 5 cut(s) 500, 760, 1366, 1631, 2102
FauI CCCGC 2 cut(s) 137, 1343
FbaI TGATCA 2 cut(s) 1153, 1537
FblI GTMKAC 4 cut(s) 180, 1009, 1511, 2542
Fnu4HI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2533
FokI GGATG 5 cut(s) 372, 563, 1559, 1717, 1962
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2533
FspBI CTAG 6 cut(s) 38, 653, 1245, 1628, 1923, 2228
GlaI GCGC 5 cut(s) 172, 223, 452, 1271, 2531
GluI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2533
GsaI CCCAGC 1 cut(s) 3204
GsuI CTGGAG 4 cut(s) 50, 1408, 1423, 3018
HaeII RGCGCY 2 cut(s) 174, 1273
HaeIII GGCC 5 cut(s) 107, 140, 325, 380, 907
HgaI GACGC 1 cut(s) 1089
HhaI GCGC 5 cut(s) 173, 224, 453, 1272, 2532
Hin1I GRCGYC 3 cut(s) 260, 1100, 2697
Hin6I GCGC 5 cut(s) 171, 222, 451, 1270, 2530
HinP1I GCGC 5 cut(s) 171, 222, 451, 1270, 2530
HincII GTYRAC 4 cut(s) 181, 258, 2379, 3330
HindII GTYRAC 4 cut(s) 181, 258, 2379, 3330
HindIII AAGCTT 1 cut(s) 859
Hpy99I CGWCG 6 cut(s) 80, 182, 265, 379, 1821, 2702
HpyAV CCTTC 9 cut(s) 776, 866, 1083, 1124, 1597, 1625, 1934, 2159, 3304
HpyCH4III ACNGT 6 cut(s) 80, 498, 1817, 1848, 2793, 2812
HpyCH4IV ACGT 6 cut(s) 120, 260, 374, 749, 2697, 3059
HpyF10VI GCNNNNNNNGC 5 cut(s) 219, 499, 636, 2527, 3320
HpySE526I ACGT 6 cut(s) 120, 260, 374, 749, 2697, 3059
Hsp92I GRCGYC 3 cut(s) 260, 1100, 2697
HspAI GCGC 5 cut(s) 171, 222, 451, 1270, 2530
KpnI GGTACC 1 cut(s) 28
KroI GCCGGC 3 cut(s) 107, 167, 286
KroNI GCCGGC 3 cut(s) 109, 169, 288
Ksp22I TGATCA 2 cut(s) 1153, 1537
LmnI GCTCC 9 cut(s) 52, 178, 295, 349, 1134, 1321, 1766, 2860, 2966
Lsp1109I GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2519
LweI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
MaeI CTAG 6 cut(s) 38, 653, 1245, 1628, 1923, 2228
MaeII ACGT 6 cut(s) 120, 260, 374, 749, 2697, 3059
MaeIII GTNAC 9 cut(s) 10, 428, 700, 1172, 1673, 2218, 2296, 2793, 3148
MflI RGATCY 3 cut(s) 1597, 1987, 2420
MhlI GDGCHC 4 cut(s) 153, 1139, 1778, 2131
MlyI GAGTC 6 cut(s) 482, 563, 1169, 1868, 1928, 3292
MmeI TCCRAC 6 cut(s) 228, 1287, 2035, 2532, 2854, 3156
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 3 cut(s) 107, 167, 286
MroXI GAANNNNTTC 1 cut(s) 682
MseI TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
MslI CAYNNNNRTG 2 cut(s) 2109, 2136
MspA1I CMGCKG 2 cut(s) 313, 1896
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 8 cut(s) 29, 104, 1778, 1779, 2121, 3133, 3216, 3325
MvaI CCWGG 3 cut(s) 29, 2121, 3133
MvnI CGCG 2 cut(s) 222, 383
MwoI GCNNNNNNNGC 5 cut(s) 219, 499, 636, 2527, 3320
NaeI GCCGGC 3 cut(s) 109, 169, 288
NciI CCSGG 5 cut(s) 104, 1778, 1779, 3216, 3325
NcoI CCATGG 1 cut(s) 1669
NgoMIV GCCGGC 3 cut(s) 107, 167, 286
NlaIV GGNNCC 6 cut(s) 26, 291, 503, 1549, 2022, 2650
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
NspI RCATGY 3 cut(s) 1526, 2030, 2108
PaeI GCATGC 1 cut(s) 1526
PaeR7I CTCGAG 4 cut(s) 134, 152, 227, 395
PceI AGGCCT 1 cut(s) 140
PciI ACATGT 1 cut(s) 2026
PcsI WCGNNNNNNNCGW 3 cut(s) 314, 323, 380
PdiI GCCGGC 3 cut(s) 109, 169, 288
PdmI GAANNNNTTC 1 cut(s) 682
PfeI GAWTC 9 cut(s) 244, 877, 1183, 1325, 1484, 1754, 2290, 2555, 2819
PflMI CCANNNNNTGG 1 cut(s) 49
PinAI ACCGGT 2 cut(s) 504, 1685
PkrI GCNGC 8 cut(s) 57, 234, 285, 312, 382, 1410, 2097, 2534
PleI GAGTC 6 cut(s) 482, 562, 1168, 1867, 1927, 3291
PpsI GAGTC 6 cut(s) 482, 562, 1168, 1867, 1927, 3291
PscI ACATGT 1 cut(s) 2026
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 27, 2119, 3131
PspEI GGTNACC 1 cut(s) 700
PspFI CCCAGC 1 cut(s) 3200
PspGI CCWGG 3 cut(s) 27, 2119, 3131
PspN4I GGNNCC 6 cut(s) 26, 291, 503, 1549, 2022, 2650
PspXI VCTCGAGB 3 cut(s) 134, 227, 395
PstI CTGCAG 1 cut(s) 1858
PstNI CAGNNNCTG 1 cut(s) 1954
PsuI RGATCY 3 cut(s) 1597, 1987, 2420
PvuII CAGCTG 1 cut(s) 1896
RsaI GTAC 2 cut(s) 26, 1471
RsaNI GTAC 2 cut(s) 25, 1470
RseI CAYNNNNRTG 2 cut(s) 2109, 2136
SacI GAGCTC 1 cut(s) 153
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
SatI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2533
SchI GAGTC 6 cut(s) 482, 563, 1169, 1868, 1928, 3292
ScrFI CCNGG 8 cut(s) 29, 104, 1778, 1779, 2121, 3133, 3216, 3325
SduI GDGCHC 4 cut(s) 153, 1139, 1778, 2131
SfaNI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
SfcI CTRYAG 3 cut(s) 1737, 1854, 1897
Sfr274I CTCGAG 4 cut(s) 134, 152, 227, 395
SgrAI CRCCGGYG 1 cut(s) 167
SlaI CTCGAG 4 cut(s) 134, 152, 227, 395
SmaI CCCGGG 1 cut(s) 1779
SmiMI CAYNNNNRTG 2 cut(s) 2109, 2136
SmlI CTYRAG 8 cut(s) 134, 152, 227, 266, 395, 1446, 2546, 3263
SmoI CTYRAG 8 cut(s) 134, 152, 227, 266, 395, 1446, 2546, 3263
SphI GCATGC 1 cut(s) 1526
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 5 cut(s) 130, 220, 311, 381, 1350
SspI AATATT 2 cut(s) 805, 2988
SspMI CTAG 6 cut(s) 38, 653, 1245, 1628, 1923, 2228
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 8 cut(s) 27, 102, 1776, 1777, 2119, 3131, 3214, 3323
StyI CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
TaaI ACNGT 6 cut(s) 80, 498, 1817, 1848, 2793, 2812
TaiI ACGT 6 cut(s) 123, 263, 377, 752, 2700, 3062
TatI WGTACW 1 cut(s) 1469
TauI GCSGC 2 cut(s) 313, 383
TfiI GAWTC 9 cut(s) 244, 877, 1183, 1325, 1484, 1754, 2290, 2555, 2819
Tru1I TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
Tru9I TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
TscAI CASTG 4 cut(s) 501, 1875, 2116, 2602
TseFI GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
TseI GCWGC 6 cut(s) 55, 232, 283, 1408, 2095, 2532
Tsp45I GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
TspDTI ATGAA 6 cut(s) 1274, 1485, 2175, 2367, 2472, 2523
TspGWI ACGGA 2 cut(s) 1968, 3276
TspMI CCCGGG 1 cut(s) 1777
TspRI CASTG 4 cut(s) 501, 1875, 2116, 2602
Van91I CCANNNNNTGG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 9 cut(s) 1714, 1839, 2073, 2080, 2459, 2511, 2659, 2840, 3179
XceI RCATGY 3 cut(s) 1526, 2030, 2108
XhoI CTCGAG 4 cut(s) 134, 152, 227, 395
XmaI CCCGGG 1 cut(s) 1777
XmiI GTMKAC 4 cut(s) 180, 1009, 1511, 2542
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 6 cut(s) 38, 653, 1245, 1628, 1923, 2228
ZraI GACGTC 2 cut(s) 261, 2698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.