Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003359

Physical Location & Seq

Genomic Coordinates Forward (+)

2462670 .. 2465275

Gene Structure 2606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005870

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGATCCAAAGATTAAACTCGGGTCGTCGGATCCTAACCTACCCGGCGGTTCATCCCTGCGAAGCCATAGCTCCGGAGACGCTTCTGATCTCACTCATCGCTCTCACCCACAACATTTGCTCTTACAAATCCAAATGCATCTCCAGCAACAAGCGAAACGCGTTGGAAGCAATCCGCCAAATCGGAGTGATTCGGGTTTTTTTGGAAGAGATCCAGGCCGGGCTGCTGCCGGGTCTTCCGGATTCGACCGTTTTGAGCTTCTCGGAGCTCCATTTGGCTTTCCAGAAGGTTCAGTACTTGCTGGAAGACTGTACCCGGGACGACGCCCGGGTGTGGATGCTGATGAATTCGGAGCGGGTTGCGAACCAGTTCCGGGTCTTGATTCGGGCCATGGCGACGGCTCTCGATGTTCTGCCTCTTGATTCGGTCGACGTGGCAGTTGAGGTGAAGGAGCAGGTGGAATTGGTGATGAGGCAAGCGCGGCGGGTGAGGTTCGAAGTTGACCCGGAAGATAAACGGGCCGTGGAGGCGGTTAAGAAAGTTTTGATCGGGTTCGAGAAACGGGTCGACCCGGATAGGAGTGAGCTGAAGCGGGTACTTGATCATGTTGGGATTCGAAGATGGAGTAGGTGCGAGAAGGAGGTGAGATTTTTGGAATCTGAAATTGGGTTTGAGAATCTGAGAGAGGTGGGTTTGCTGTGCAGCCTGAAGGGGTTGTTGTGCTACTGCCGGTGCGTCATGTTCGGGTCTGTGGACGGGTTACCCCCCGGCCCGCGACCCGAGCCCAGTAGCCGCAGCGAGCTGGTGAGTAGCCTGAATTTGGAAGATTTCCGGTGTCCGATATCGTTGGAGATAATGACGGATCCGGTGACGATCGGGACGGGTCACAGCTACGATCGGAGCTCCATTATGAAGTGGTTCAGAGGTGGAAAGCCGATTTGCCCGAAAACAGGGGAGAAGATGAAGAGCACGGAGGTGGTGCCTAATTTGGCATTGAAGAGGTTGATTCAGCAGTACTGCAATGAGAATGGGATTCCGTTTGGCGATTCGGGTCGTCGGAATCGCGACATTAGGAGGAGTACAGTTGCAGGTAGCTTGGCGGCTGAGGGAGCTATGAAAATGGTGGCGGAGTTTCTCTGTGGAAGTGTTGGGAGTGGATCGAATAAGGCTGCTTATGAGATTCGGCTTCTTTCGAAAACAAGCATTTTTAATAGGTCTTGTTTGGTGGAAGCTGGAGTGGTGCCGCATTTGTTGAAGCTGTTGGCTTCTGCAGATTCGGAGACTCAAGAGAATGCAGTTGCAGGGTTGTTGAATCTGTCCAAGCATTCGAAAGGGAAAGCTGTGATTGTTGAAAATGGGGGTGTGGATTTGGTTGTGGATGTTCTCAAGGAGGGTCTCAAAGTTGAGGCTTGCCAGCATGCTGCTGGCACTTTGTTTTATCTTGCTTCGATTGAGGAGTACCGGATATTGATCGGAGAAAACCCGAGGGCCATACCGGCTTTGAGGGAGCTGATCAAGAACGGTTCTGATCGAGGCAAGAAGAATGCATTGGTTGCTATATTCGGGCTGCTTACACACCCTGCAAATCATACAAGGGTGTTAGCAGCTGGGGTGGTTCCTTTGCTGTTTGAGATACTGACATCTTTTGACAGTGAAGTTCTTGTGACAGATTCTCTAGCAATTCTCGCAACTCTGGCTGAGAAACCCGAGGGAGCAGTTGCAATTTCGCGTTGCAAGGCTTTGGATTCGATTGTGGGGATTCTGAATTCTTCGACTTCAAGGGCAGCGAAGGAGTTCTGTGTGTCTTTGTTGCTGGCTCTTTGTGCAAATGGTGGCCACAATGTGGTTGCTCTTTTGGTTAAGAGCCCTTCTCTTATGGGATGTTTGTATTCCCAACTTAGTGAAGGCACATCTAGAGCAAGCAAAAGGGCCAGTGCTCTCATCAAGGTCCTCCATGAATTCCATGAAACAAGATCGTCTGGTCCACTCACTCGGGTTCTTCCACAGGAGCGATTTGTTCACGTTAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009966 GO:0009987 GO:0010029 GO:0010119 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1900140 GO:1901419 GO:1901564 GO:1905957 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

677

Amino Acids

74.18

Weight (kDa)

8.8

Isoelectric Point (pI)

42.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	53 - 162	4.7e-14	PUB10-like, N-terminal domain
U-box	PF04564	275 - 343	6.1e-17	U-box domain
zf-RING_UBOX	PF13445	279 - 315	8.9e-06	RING-type zinc-finger
ARM_ARMC5	PF24768	361 - 552	1.4e-09	ARMC5
ARM_PUB	PF25598	389 - 651	1.5e-14	Plant U-box protein ARM repeat region
Arm	PF00514	404 - 440	2.7e-09	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10560 AT1G60190
fragaria_vesca	FvH4_4g34620 FvH4_5g05140
malus_domestica	MD06G1177100.v1.1 MD13G1090900.v1.1 MD14G1183300.v1.1 MD16G1091300.v1.1
prunus_persica	Prupe.1G255000_v2.0.a1 Prupe.5G175800_v2.0.a1
pyrus_communis	pycom06g15720 pycom13g07890 pycom14g15220 pycom16g07800
rosa_chinensis	RchiOBHm_Chr4g0443981 RchiOBHm_Chr7g0193831
rosa_laevigata	RLG00000004242 RLG00000005870
rosa_multiflora	Rmu_co8252843.1_g000001 Rmu_sc0001436.1_g000032 Rmu_sc0027702.1_g000002 Rmu_sc0029481.1_g000002
rosa_roxburghii	Rroxscaffold_3G00261790 Rroxscaffold_5G00384530
rosa_rugosa	Rorug04G0348700 Rorug07G0012400
rosa_samantha	Rh4AG409400 Rh4BG420500 Rh4CG434500 Rh4DG415500 Rh7AG139000 Rh7BG139200 Rh7CG142900 Rh7DG140500
rosa_wichuraiana	Rw4G035170 Rw7G011850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	445
Acc36I	ACCTGC	2 cut(s)	445, 1079
AccB1I	GGYRCC	2 cut(s)	979, 1240
AccB7I	CCANNNNNTGG	1 cut(s)	1843
AccBSI	CCGCTC	1 cut(s)	355
AccI	GTMKAC	2 cut(s)	429, 567
AccII	CGCG	5 cut(s)	161, 481, 775, 1065, 1729
AccIII	TCCGGA	2 cut(s)	73, 238
AclWI	GGATC	6 cut(s)	25, 38, 205, 857, 870, 1165
AcoI	YGGCCR	1 cut(s)	1834
AcsI	RAATTY	4 cut(s)	346, 817, 1765, 1958
AcuI	CTGAAG	2 cut(s)	608, 728
AcyI	GRCGYC	1 cut(s)	324
AdeI	CACNNNGTG	1 cut(s)	1843
AfaI	GTAC	6 cut(s)	296, 313, 597, 1016, 1081, 1460
AfiI	CCNNNNNNNGG	5 cut(s)	333, 373, 820, 950, 1843
AflIII	ACRYGT	1 cut(s)	159
AgsI	TTSAA	5 cut(s)	997, 1255, 1312, 1352, 1779
AjiI	CACGTC	1 cut(s)	433
AjnI	CCWGG	1 cut(s)	213
AjuI	GAANNNNNNNTTGG	4 cut(s)	648, 680, 1532, 1564
AleI	CACNNNNGTG	1 cut(s)	974
Alw21I	GWGCWC	4 cut(s)	270, 905, 971, 1939
Alw26I	GTCTC	3 cut(s)	71, 1274, 1400
AlwI	GGATC	6 cut(s)	25, 38, 205, 857, 870, 1165
Ama87I	CYCGRG	7 cut(s)	19, 315, 327, 779, 1483, 1706, 1992
Aor13HI	TCCGGA	2 cut(s)	73, 238
AoxI	GGCC	7 cut(s)	216, 387, 519, 769, 1488, 1834, 1929
ApeKI	GCWGC	9 cut(s)	223, 226, 702, 795, 1169, 1421, 1567, 1604, 1784
ApoI	RAATTY	4 cut(s)	346, 817, 1765, 1958
Asp700I	GAANNNNTTC	3 cut(s)	368, 917, 1793
AspLEI	GCGC	1 cut(s)	481
AspS9I	GGNCC	7 cut(s)	387, 519, 770, 1488, 1929, 1948, 1982
AsuHPI	GGTGA	7 cut(s)	97, 457, 478, 499, 655, 817, 880
AsuII	TTCGAA	4 cut(s)	495, 616, 1193, 1328
AvaI	CYCGRG	7 cut(s)	19, 315, 327, 779, 1483, 1706, 1992
AvaII	GGWCC	2 cut(s)	1948, 1982
BalI	TGGCCA	1 cut(s)	1836
BamHI	GGATCC	2 cut(s)	30, 862
BanI	GGYRCC	2 cut(s)	979, 1240
BanII	GRGCYC	4 cut(s)	270, 786, 905, 1868
BarI	GAAGNNNNNNTAC	2 cut(s)	278, 310
BbsI	GAAGAC	2 cut(s)	227, 312
Bbv12I	GWGCWC	4 cut(s)	270, 905, 971, 1939
BbvCI	CCTCAGC	1 cut(s)	1104
BbvI	GCAGC	9 cut(s)	210, 213, 714, 807, 1156, 1408, 1554, 1616, 1796
BccI	CCATC	1 cut(s)	615
BceAI	ACGGC	2 cut(s)	414, 506
BciT130I	CCWGG	1 cut(s)	215
BclI	TGATCA	2 cut(s)	601, 1512
BcoDI	GTCTC	3 cut(s)	71, 1274, 1400
BfaI	CTAG	2 cut(s)	1676, 1914
BfmI	CTRYAG	1 cut(s)	1269
BfuAI	ACCTGC	2 cut(s)	445, 1079
BglI	GCCNNNNNGGC	2 cut(s)	527, 1496
BmcAI	AGTACT	2 cut(s)	296, 1016
Bme18I	GGWCC	2 cut(s)	1948, 1982
BmeT110I	CYCGRG	7 cut(s)	19, 315, 327, 779, 1483, 1706, 1992
BmgBI	CACGTC	1 cut(s)	433
BmgT120I	GGNCC	7 cut(s)	387, 519, 770, 1488, 1929, 1948, 1982
BmiI	GGNNCC	5 cut(s)	32, 864, 981, 1242, 1617
BmrI	ACTGGG	1 cut(s)	780
BmsI	GCATC	2 cut(s)	147, 327
BmuI	ACTGGG	1 cut(s)	780
BpiI	GAAGAC	2 cut(s)	227, 312
BplI	GAGNNNNNCTC	2 cut(s)	1855, 1887
BpmI	CTGGAG	2 cut(s)	127, 1254
Bpu10I	CCTNAGC	1 cut(s)	1104
Bpu14I	TTCGAA	4 cut(s)	495, 616, 1193, 1328
BpuEI	CTTGAG	2 cut(s)	1269, 1370
BsaBI	GATNNNNATC	1 cut(s)	1469
BsaHI	GRCGYC	1 cut(s)	324
BsaI	GGTCTC	1 cut(s)	1400
BsaJI	CCNNGG	7 cut(s)	315, 327, 390, 522, 766, 1484, 1707
BsaWI	WCCGGW	5 cut(s)	73, 238, 831, 865, 1461
Bsc4I	CCNNNNNNNGG	5 cut(s)	333, 373, 820, 950, 1843
Bse118I	RCCGGY	2 cut(s)	729, 1495
Bse1I	ACTGG	3 cut(s)	367, 786, 1932
Bse3DI	GCAATG	1 cut(s)	1027
Bse8I	GATNNNNATC	1 cut(s)	1469
BseAI	TCCGGA	2 cut(s)	73, 238
BseBI	CCWGG	1 cut(s)	215
BseDI	CCNNGG	7 cut(s)	315, 327, 390, 522, 766, 1484, 1707
BseGI	GGATG	4 cut(s)	52, 342, 1384, 1886
BseJI	GATNNNNATC	1 cut(s)	1469
BseLI	CCNNNNNNNGG	5 cut(s)	333, 373, 820, 950, 1843
BseMI	GCAATG	1 cut(s)	1027
BseMII	CTCAG	3 cut(s)	671, 1095, 1689
BseNI	ACTGG	3 cut(s)	367, 786, 1932
BseRI	GAGGAG	2 cut(s)	1090, 1469
BseXI	GCAGC	9 cut(s)	210, 213, 714, 807, 1156, 1408, 1554, 1616, 1796
BseYI	CCCAGC	1 cut(s)	1607
BsgI	GTGCAG	1 cut(s)	721
Bsh1236I	CGCG	5 cut(s)	161, 481, 775, 1065, 1729
Bsh1285I	CGRYCG	4 cut(s)	249, 429, 876, 898
BshFI	GGCC	7 cut(s)	218, 389, 521, 771, 1490, 1836, 1931
BshNI	GGYRCC	2 cut(s)	979, 1240
BsiEI	CGRYCG	4 cut(s)	249, 429, 876, 898
BsiHKAI	GWGCWC	4 cut(s)	270, 905, 971, 1939
BsiHKCI	CYCGRG	7 cut(s)	19, 315, 327, 779, 1483, 1706, 1992
BslFI	GGGAC	2 cut(s)	332, 892
BslI	CCNNNNNNNGG	5 cut(s)	333, 373, 820, 950, 1843
BsmAI	GTCTC	3 cut(s)	71, 1274, 1400
BsmBI	CGTCTC	1 cut(s)	71
BsmFI	GGGAC	2 cut(s)	332, 892
BsmI	GAATGC	3 cut(s)	1297, 1324, 1549
BsnI	GGCC	7 cut(s)	218, 389, 521, 771, 1490, 1836, 1931
Bso31I	GGTCTC	1 cut(s)	1400
BsoBI	CYCGRG	7 cut(s)	19, 315, 327, 779, 1483, 1706, 1992
Bsp119I	TTCGAA	4 cut(s)	495, 616, 1193, 1328
Bsp1286I	GDGCHC	6 cut(s)	270, 786, 905, 971, 1868, 1939
Bsp13I	TCCGGA	2 cut(s)	73, 238
Bsp19I	CCATGG	1 cut(s)	390
Bsp68I	TCGCGA	1 cut(s)	1065
BspANI	GGCC	7 cut(s)	218, 389, 521, 771, 1490, 1836, 1931
BspCNI	CTCAG	3 cut(s)	672, 1096, 1690
BspEI	TCCGGA	2 cut(s)	73, 238
BspFNI	CGCG	5 cut(s)	161, 481, 775, 1065, 1729
BspLI	GGNNCC	5 cut(s)	32, 864, 981, 1242, 1617
BspMAI	CTGCAG	1 cut(s)	1273
BspMI	ACCTGC	2 cut(s)	445, 1079
BspPI	GGATC	6 cut(s)	25, 38, 205, 857, 870, 1165
BspQI	GCTCTTC	1 cut(s)	959
BspT104I	TTCGAA	4 cut(s)	495, 616, 1193, 1328
BspT107I	GGYRCC	2 cut(s)	979, 1240
BspTNI	GGTCTC	1 cut(s)	1400
BsrBI	CCGCTC	1 cut(s)	355
BsrDI	GCAATG	1 cut(s)	1027
BsrFI	RCCGGY	2 cut(s)	729, 1495
BsrI	ACTGG	3 cut(s)	367, 786, 1932
BssAI	RCCGGY	2 cut(s)	729, 1495
BssECI	CCNNGG	7 cut(s)	315, 327, 390, 522, 766, 1484, 1707
BssNI	GRCGYC	1 cut(s)	324
BssT1I	CCWWGG	1 cut(s)	390
Bst2UI	CCWGG	1 cut(s)	215
Bst4CI	ACNGT	5 cut(s)	250, 311, 1084, 1523, 1652
Bst6I	CTCTTC	3 cut(s)	201, 959, 992
BstACI	GRCGYC	1 cut(s)	324
BstAPI	GCANNNNNTGC	1 cut(s)	1553
BstBI	TTCGAA	4 cut(s)	495, 616, 1193, 1328
BstC8I	GCNNGC	9 cut(s)	477, 773, 800, 1411, 1415, 1419, 1426, 1815, 1921
BstDEI	CTNAG	4 cut(s)	680, 1104, 1698, 1898
BstDSI	CCRYGG	2 cut(s)	390, 522
BstEII	GGTNACC	1 cut(s)	759
BstF5I	GGATG	4 cut(s)	52, 342, 1384, 1886
BstFNI	CGCG	5 cut(s)	161, 481, 775, 1065, 1729
BstHHI	GCGC	1 cut(s)	481
BstMAI	GTCTC	3 cut(s)	71, 1274, 1400
BstMCI	CGRYCG	4 cut(s)	249, 429, 876, 898
BstNI	CCWGG	1 cut(s)	215
BstNSI	RCATGY	1 cut(s)	1421
BstPI	GGTNACC	1 cut(s)	759
BstSFI	CTRYAG	1 cut(s)	1269
BstUI	CGCG	5 cut(s)	161, 481, 775, 1065, 1729
BstV1I	GCAGC	9 cut(s)	210, 213, 714, 807, 1156, 1408, 1554, 1616, 1796
BstV2I	GAAGAC	2 cut(s)	227, 312
BstX2I	RGATCY	3 cut(s)	30, 210, 862
BstYI	RGATCY	3 cut(s)	30, 210, 862
BsuRI	GGCC	7 cut(s)	218, 389, 521, 771, 1490, 1836, 1931
BtgI	CCRYGG	2 cut(s)	390, 522
BtgZI	GCGATG	1 cut(s)	82
BtrI	CACGTC	1 cut(s)	433
BtsCI	GGATG	4 cut(s)	52, 342, 1384, 1886
BtsIMutI	CAGTG	2 cut(s)	1657, 1939
BtuMI	TCGCGA	1 cut(s)	1065
BveI	ACCTGC	2 cut(s)	445, 1079
Cac8I	GCNNGC	9 cut(s)	477, 773, 800, 1411, 1415, 1419, 1426, 1815, 1921
CfoI	GCGC	1 cut(s)	481
Cfr10I	RCCGGY	2 cut(s)	729, 1495
Cfr13I	GGNCC	7 cut(s)	387, 519, 770, 1488, 1929, 1948, 1982
Cfr9I	CCCGGG	2 cut(s)	315, 327
CseI	GACGC	3 cut(s)	88, 332, 724
Csp6I	GTAC	6 cut(s)	295, 312, 596, 1015, 1080, 1459
CspCI	CAANNNNNGTGG	2 cut(s)	98, 133
CviAII	CATG	6 cut(s)	391, 605, 739, 1418, 1955, 1964
CviQI	GTAC	6 cut(s)	295, 312, 596, 1015, 1080, 1459
DdeI	CTNAG	4 cut(s)	680, 1104, 1698, 1898
DraIII	CACNNNGTG	1 cut(s)	1843
EaeI	YGGCCR	1 cut(s)	1834
Eam1104I	CTCTTC	3 cut(s)	201, 959, 992
EarI	CTCTTC	3 cut(s)	201, 959, 992
EciI	GGCGGA	2 cut(s)	164, 1142
Ecl136II	GAGCTC	2 cut(s)	268, 903
Eco130I	CCWWGG	1 cut(s)	390
Eco24I	GRGCYC	4 cut(s)	270, 786, 905, 1868
Eco31I	GGTCTC	1 cut(s)	1400
Eco32I	GATATC	1 cut(s)	843
Eco47I	GGWCC	2 cut(s)	1948, 1982
Eco53kI	GAGCTC	2 cut(s)	268, 903
Eco57I	CTGAAG	2 cut(s)	608, 728
Eco88I	CYCGRG	7 cut(s)	19, 315, 327, 779, 1483, 1706, 1992
Eco91I	GGTNACC	1 cut(s)	759
EcoICRI	GAGCTC	2 cut(s)	268, 903
EcoO109I	RGGNCCY	1 cut(s)	1948
EcoO65I	GGTNACC	1 cut(s)	759
EcoRI	GAATTC	3 cut(s)	346, 1765, 1958
EcoRII	CCWGG	1 cut(s)	213
EcoRV	GATATC	1 cut(s)	843
EcoT14I	CCWWGG	1 cut(s)	390
EcoT22I	ATGCAT	2 cut(s)	140, 1549
EcoT38I	GRGCYC	4 cut(s)	270, 786, 905, 1868
ErhI	CCWWGG	1 cut(s)	390
Esp3I	CGTCTC	1 cut(s)	71
FaeI	CATG	6 cut(s)	394, 608, 742, 1421, 1958, 1967
FaqI	GGGAC	2 cut(s)	332, 892
FatI	CATG	6 cut(s)	390, 604, 738, 1417, 1954, 1963
FauI	CCCGC	4 cut(s)	348, 477, 585, 780
FbaI	TGATCA	2 cut(s)	601, 1512
FblI	GTMKAC	2 cut(s)	429, 567
FokI	GGATG	4 cut(s)	39, 349, 1391, 1893
FriOI	GRGCYC	4 cut(s)	270, 786, 905, 1868
FspBI	CTAG	2 cut(s)	1676, 1914
GlaI	GCGC	1 cut(s)	480
GsaI	CCCAGC	1 cut(s)	1611
GsuI	CTGGAG	2 cut(s)	127, 1254
HaeIII	GGCC	7 cut(s)	218, 389, 521, 771, 1490, 1836, 1931
HgaI	GACGC	3 cut(s)	88, 332, 724
HhaI	GCGC	1 cut(s)	481
Hin1I	GRCGYC	1 cut(s)	324
Hin1II	CATG	6 cut(s)	394, 608, 742, 1421, 1958, 1967
Hin6I	GCGC	1 cut(s)	479
HinP1I	GCGC	1 cut(s)	479
HincII	GTYRAC	3 cut(s)	430, 502, 568
HindII	GTYRAC	3 cut(s)	430, 502, 568
HphI	GGTGA	7 cut(s)	97, 457, 478, 499, 655, 817, 880
Hpy166II	GTNNAC	6 cut(s)	430, 502, 568, 754, 1985, 2020
Hpy8I	GTNNAC	6 cut(s)	430, 502, 568, 754, 1985, 2020
Hpy99I	CGWCG	5 cut(s)	30, 326, 400, 434, 1059
HpyAV	CCTTC	7 cut(s)	280, 442, 631, 703, 1783, 1878, 1898
HpyCH4III	ACNGT	5 cut(s)	250, 311, 1084, 1523, 1652
HpyCH4IV	ACGT	2 cut(s)	432, 2022
HpyF3I	CTNAG	4 cut(s)	680, 1104, 1698, 1898
HpySE526I	ACGT	2 cut(s)	432, 2022
Hsp92I	GRCGYC	1 cut(s)	324
Hsp92II	CATG	6 cut(s)	394, 608, 742, 1421, 1958, 1967
HspAI	GCGC	1 cut(s)	479
Kpn2I	TCCGGA	2 cut(s)	73, 238
Ksp22I	TGATCA	2 cut(s)	601, 1512
LguI	GCTCTTC	1 cut(s)	959
Lsp1109I	GCAGC	9 cut(s)	210, 213, 714, 807, 1156, 1408, 1554, 1616, 1796
LweI	GCATC	2 cut(s)	147, 327
MaeI	CTAG	2 cut(s)	1676, 1914
MaeII	ACGT	2 cut(s)	432, 2022
MaeIII	GTNAC	4 cut(s)	759, 868, 884, 1663
MbiI	CCGCTC	1 cut(s)	355
MflI	RGATCY	3 cut(s)	30, 210, 862
MhlI	GDGCHC	6 cut(s)	270, 786, 905, 971, 1868, 1939
MlsI	TGGCCA	1 cut(s)	1836
MluCI	AATT	9 cut(s)	346, 461, 663, 817, 985, 1680, 1722, 1765, 1958
MluI	ACGCGT	1 cut(s)	159
MluNI	TGGCCA	1 cut(s)	1836
MlyI	GAGTC	1 cut(s)	1276
MmeI	TCCRAC	4 cut(s)	8, 144, 828, 1037
Mox20I	TGGCCA	1 cut(s)	1836
Mph1103I	ATGCAT	2 cut(s)	140, 1549
MroI	TCCGGA	2 cut(s)	73, 238
MroXI	GAANNNNTTC	3 cut(s)	368, 917, 1793
MscI	TGGCCA	1 cut(s)	1836
MseI	TTAA	4 cut(s)	14, 534, 1209, 1860
MslI	CAYNNNNRTG	1 cut(s)	974
Msp20I	TGGCCA	1 cut(s)	1836
MspA1I	CMGCKG	1 cut(s)	1607
Mva1269I	GAATGC	3 cut(s)	1297, 1324, 1549
MvaI	CCWGG	1 cut(s)	215
MvnI	CGCG	5 cut(s)	161, 481, 775, 1065, 1729
NcoI	CCATGG	1 cut(s)	390
NlaIII	CATG	6 cut(s)	394, 608, 742, 1421, 1958, 1967
NlaIV	GGNNCC	5 cut(s)	32, 864, 981, 1242, 1617
NmuCI	GTSAC	3 cut(s)	868, 884, 1663
NruI	TCGCGA	1 cut(s)	1065
NsiI	ATGCAT	2 cut(s)	140, 1549
NspI	RCATGY	1 cut(s)	1421
NspV	TTCGAA	4 cut(s)	495, 616, 1193, 1328
OliI	CACNNNNGTG	1 cut(s)	974
PaeI	GCATGC	1 cut(s)	1421
PaqCI	CACCTGC	1 cut(s)	445
PciSI	GCTCTTC	1 cut(s)	959
PctI	GAATGC	3 cut(s)	1297, 1324, 1549
PdmI	GAANNNNTTC	3 cut(s)	368, 917, 1793
PflMI	CCANNNNNTGG	1 cut(s)	1843
Ple19I	CGATCG	2 cut(s)	876, 898
PleI	GAGTC	1 cut(s)	1276
PpsI	GAGTC	1 cut(s)	1276
PpuMI	RGGWCCY	1 cut(s)	1948
Psp124BI	GAGCTC	2 cut(s)	270, 905
Psp5II	RGGWCCY	1 cut(s)	1948
Psp6I	CCWGG	1 cut(s)	213
PspEI	GGTNACC	1 cut(s)	759
PspFI	CCCAGC	1 cut(s)	1607
PspGI	CCWGG	1 cut(s)	213
PspN4I	GGNNCC	5 cut(s)	32, 864, 981, 1242, 1617
PspPI	GGNCC	7 cut(s)	387, 519, 770, 1488, 1929, 1948, 1982
PspPPI	RGGWCCY	1 cut(s)	1948
PstI	CTGCAG	1 cut(s)	1273
PsuI	RGATCY	3 cut(s)	30, 210, 862
PvuI	CGATCG	2 cut(s)	876, 898
PvuII	CAGCTG	1 cut(s)	1607
RruI	TCGCGA	1 cut(s)	1065
RsaI	GTAC	6 cut(s)	296, 313, 597, 1016, 1081, 1460
RsaNI	GTAC	6 cut(s)	295, 312, 596, 1015, 1080, 1459
RseI	CAYNNNNRTG	1 cut(s)	974
SacI	GAGCTC	2 cut(s)	270, 905
SalI	GTCGAC	2 cut(s)	428, 566
SapI	GCTCTTC	1 cut(s)	959
SaqAI	TTAA	4 cut(s)	14, 534, 1209, 1860
Sau96I	GGNCC	7 cut(s)	387, 519, 770, 1488, 1929, 1948, 1982
ScaI	AGTACT	2 cut(s)	296, 1016
SchI	GAGTC	1 cut(s)	1276
SduI	GDGCHC	6 cut(s)	270, 786, 905, 971, 1868, 1939
SfaNI	GCATC	2 cut(s)	147, 327
SfcI	CTRYAG	1 cut(s)	1269
SfuI	TTCGAA	4 cut(s)	495, 616, 1193, 1328
SinI	GGWCC	2 cut(s)	1948, 1982
SmaI	CCCGGG	2 cut(s)	317, 329
SmiMI	CAYNNNNRTG	1 cut(s)	974
SmlI	CTYRAG	2 cut(s)	1284, 1385
SmoI	CTYRAG	2 cut(s)	1284, 1385
SphI	GCATGC	1 cut(s)	1421
Sse9I	AATT	9 cut(s)	346, 461, 663, 817, 985, 1680, 1722, 1765, 1958
SspMI	CTAG	2 cut(s)	1676, 1914
SstI	GAGCTC	2 cut(s)	270, 905
StyI	CCWWGG	1 cut(s)	390
TaaI	ACNGT	5 cut(s)	250, 311, 1084, 1523, 1652
TaiI	ACGT	2 cut(s)	435, 2025
TaqII	GACCGA	1 cut(s)	415
TasI	AATT	9 cut(s)	346, 461, 663, 817, 985, 1680, 1722, 1765, 1958
TatI	WGTACW	3 cut(s)	294, 1014, 1079
TauI	GCSGC	4 cut(s)	484, 795, 1103, 1246
Tru1I	TTAA	4 cut(s)	14, 534, 1209, 1860
Tru9I	TTAA	4 cut(s)	14, 534, 1209, 1860
TscAI	CASTG	2 cut(s)	1657, 1939
TseFI	GTSAC	3 cut(s)	868, 884, 1663
TseI	GCWGC	9 cut(s)	223, 226, 702, 795, 1169, 1421, 1567, 1604, 1784
Tsp45I	GTSAC	3 cut(s)	868, 884, 1663
TspDTI	ATGAA	7 cut(s)	41, 359, 926, 977, 1130, 1971, 1980
TspGWI	ACGGA	3 cut(s)	875, 986, 1026
TspMI	CCCGGG	2 cut(s)	315, 327
TspRI	CASTG	2 cut(s)	1657, 1939
Van91I	CCANNNNNTGG	1 cut(s)	1843
VpaK11BI	GGWCC	2 cut(s)	1948, 1982
XapI	RAATTY	4 cut(s)	346, 817, 1765, 1958
XbaI	TCTAGA	1 cut(s)	1913
XceI	RCATGY	1 cut(s)	1421
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1421
XmaI	CCCGGG	2 cut(s)	315, 327
XmiI	GTMKAC	2 cut(s)	429, 567
XmnI	GAANNNNTTC	3 cut(s)	368, 917, 1793
XspI	CTAG	2 cut(s)	1676, 1914
ZrmI	AGTACT	2 cut(s)	296, 1016
Zsp2I	ATGCAT	2 cut(s)	140, 1549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.