Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003359

Physical Location & Seq

Genomic Coordinates Reverse (-)

61987492 .. 61989812

Gene Structure 2321 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G035170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2028 bp

ATGATCCAAAGCTTAAACTCGGGTCGTCGGATCCTAACCTACCCGGCGGTTCATCCCTGCGAAGCCATAGCTCCGGAGACGCTTCTGACCTCACTCATCGCTCTCACCCACAGCATTTGCTCTTACAAATCCAAATGCATCGCCAGCAACAAGCGAAACGCTTTGGAAGCAATCCGCCAAATCGGAGTGATTCGGGATTTTTTGGAAGAGATCCAGGCCGGGCCGCCGGGTCTTCCGGATTCGACCGTTTTGAGCTTCTCGGAGCTCCATTTGGCTTTCCAGAAGGTTCAGTACTTGCTGGAAGACTGTACCCGGGACGACGCCCGGCTATGGATGCTGATGAATTCGGAGCGGGTTGCGAACCAGTTCCGGGTCTTGATTCGGGCCATGGCGACGGCTCTCGATGTTCTGCCTCTTGATGTCATCCACGTGGCAGTTGAGGTGAAGGAGCAGGTCGAATTGGTGATGAGGCAAGCGCGGCGGGTGAGGTTCCAAGTTGACCCGGAAGATAAGCGGGTCGTCGAGGCGGTTAAGAAAGTTTTGATCGGGTTCGAGAAACGGGTCGACCCGGATAGGAGTGAGCTGAAGCGGGTACTTGATCATGGTGGGATTCGGAGATGGAGTAGGTGCGAGAAGGAGGTGAGATTTTTGGAATCTGAAATTGGGTTCGAGAATCTGAGAGAGGTGGGGTTGCTGAGCAGCCTGAAGGGGTTGTTGTGCTACTGCCGGTGCGTCATGTTCGGGTCTGTGGACGGGTTACCCGGCCCGCGACCCGAGCCCAGTAGCCGCAGCGAGGTGGTGGTGAGTAGCCTGAATGTGGAAGATTTCCGGTGCCCGATATCGCTGGAGATAATGACGGATCCGGTGACGATCGGGACGGGTCACAGCTACGACCGGAGCTCCATTATGAAGTGGTTCGGAGCCGGAAAGCCGATTTGCCCGAAAACAGGGGAGAAAATGAAGAGCACGGGGGTGGTGCCTAATTTGGCACTGAAGAGGCTGATCCAGCAGTACTGCAGTGAGAATGGGATTCCGTTTGGCGATTCGGGTCGTCGGAATCGCGACACTGGGAGGAGTACAGCTGCAGGTAGCTCGGCGGCCGAGGGAGCTATGAAGATGGTGGCGGAGTTTCTCTGTGCGAGTGTTGGGAGTGGATCGAATAAGGCTGCTTATGAGATTCGGCTTCTTTCGAAAACAAGCATTTTTAACAGGTCTTGTTTGGTGGAAGCTGGAGTGGTGCCGCATTTGTTGAAGCTGTTGGCTTGTGCAGATTCGGAGACTCAAGAGAATGCAGTTGCAGGGTTGTTGAATCTGTCCAAGCATTCGAAAGGGAAAGCTGTGATTGTTGAAAATGGGGGTGTGGATTTGGTTGTGGATGTTCTCAAGGAGGGTCTCAAAGTTGAGGCTTGCCAGCATGCTGCTGGCACTTTGTTTTATCTTGCTTCCATTGAGGAGTACCGGATATTGATCGGAGAAAACCCGAGGGCCATACCGGCTCTGAGGGAGCTGATCAACAACGGTTCTGATCGAGGCAAGAAGAATGCATTGGTTGCTATATTCGGCCTGCTTACACACCCTGCAAATCATACGAGGGTGTTAGCAGCTGGGGTGGTTCCTTTGCTGTTTGAGATACTGACGTCTTTTGACAGTGAAGTTCTTGTGACAGATTCTCTAGCGATTCTCGCAACTCTGGCTGAGAAACCCGAGGGAGCAGTTGCAATTTCGCGTTGCAAGGCTTTGGATTCCATTGTGGGGATTCTGAATTCTTCGACTTCAAGGGCGTCCAAGGAGTTCTGTGTGTCTTTGTTGCTGGCTCTTTGTGCAAATGGTGGCCACAATGTGGTTGCTCTTTTGGTTAAGAGCCCTTCTCTTATGGCATGTTTGTATTCCCAACTTAGTGAAGGCACATCTAGAGCAAGCAAAAGGGCCGGTGCTCTCATCAAGGTCCTCCATGAATTCCATGAAACAAGATCGTCTGGTCCAGTCACTCGGGTTCTTCCACAGGAGCGATTTGTTCACGTTAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009966 GO:0009987 GO:0010029 GO:0010119 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1900140 GO:1901419 GO:1901564 GO:1905957 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

675

Amino Acids

73.6

Weight (kDa)

8.67

Isoelectric Point (pI)

43.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	53 - 161	4.4e-14	PUB10-like, N-terminal domain
U-box	PF04564	274 - 342	4.4e-16	U-box domain
ARM_ARMC5	PF24768	367 - 551	2.8e-08	ARMC5
ARM_PUB	PF25598	387 - 619	4.8e-14	Plant U-box protein ARM repeat region
Arm	PF00514	403 - 439	7.2e-09	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10560 AT1G60190
fragaria_vesca	FvH4_4g34620 FvH4_5g05140
malus_domestica	MD06G1177100.v1.1 MD13G1090900.v1.1 MD14G1183300.v1.1 MD16G1091300.v1.1
prunus_persica	Prupe.1G255000_v2.0.a1 Prupe.5G175800_v2.0.a1
pyrus_communis	pycom06g15720 pycom13g07890 pycom14g15220 pycom16g07800
rosa_chinensis	RchiOBHm_Chr4g0443981 RchiOBHm_Chr7g0193831
rosa_laevigata	RLG00000004242 RLG00000005870
rosa_multiflora	Rmu_co8252843.1_g000001 Rmu_sc0001436.1_g000032 Rmu_sc0027702.1_g000002 Rmu_sc0029481.1_g000002
rosa_roxburghii	Rroxscaffold_3G00261790 Rroxscaffold_5G00384530
rosa_rugosa	Rorug04G0348700 Rorug07G0012400
rosa_samantha	Rh4AG409400 Rh4BG420500 Rh4CG434500 Rh4DG415500 Rh7AG139000 Rh7BG139200 Rh7CG142900 Rh7DG140500
rosa_wichuraiana	Rw4G035170 Rw7G011850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1640
Acc36I	ACCTGC	2 cut(s)	442, 1076
AccB1I	GGYRCC	3 cut(s)	831, 976, 1237
AccB7I	CCANNNNNTGG	1 cut(s)	1840
AccBSI	CCGCTC	1 cut(s)	352
AccI	GTMKAC	1 cut(s)	564
AccII	CGCG	4 cut(s)	478, 769, 1062, 1726
AccIII	TCCGGA	2 cut(s)	73, 235
AclWI	GGATC	7 cut(s)	25, 38, 205, 854, 867, 997, 1162
AcoI	YGGCCR	2 cut(s)	1098, 1831
AcsI	RAATTY	3 cut(s)	343, 1762, 1955
AcuI	CTGAAG	3 cut(s)	605, 725, 1013
AcvI	CACGTG	1 cut(s)	430
AcyI	GRCGYC	3 cut(s)	321, 1637, 1781
AdeI	CACNNNGTG	1 cut(s)	1840
AfaI	GTAC	6 cut(s)	293, 310, 594, 1013, 1078, 1457
AfiI	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 817, 947, 1752, 1840
AgsI	TTSAA	4 cut(s)	1252, 1309, 1349, 1776
AjnI	CCWGG	1 cut(s)	213
AjuI	GAANNNNNNNTTGG	4 cut(s)	645, 677, 1529, 1561
AleI	CACNNNNGTG	1 cut(s)	971
Alw21I	GWGCWC	4 cut(s)	267, 902, 968, 1936
Alw26I	GTCTC	3 cut(s)	71, 1271, 1397
AlwI	GGATC	7 cut(s)	25, 38, 205, 854, 867, 997, 1162
Ama87I	CYCGRG	6 cut(s)	19, 312, 773, 1480, 1703, 1989
Aor13HI	TCCGGA	2 cut(s)	73, 235
AoxI	GGCC	9 cut(s)	216, 221, 384, 763, 1098, 1485, 1561, 1831, 1926
ApeKI	GCWGC	6 cut(s)	699, 789, 1082, 1166, 1418, 1601
ApoI	RAATTY	3 cut(s)	343, 1762, 1955
Asp700I	GAANNNNTTC	2 cut(s)	365, 914
AspLEI	GCGC	1 cut(s)	478
AspS9I	GGNCC	7 cut(s)	221, 384, 764, 1485, 1926, 1945, 1979
AsuHPI	GGTGA	7 cut(s)	97, 454, 475, 496, 652, 814, 877
AsuII	TTCGAA	2 cut(s)	1190, 1325
AvaI	CYCGRG	6 cut(s)	19, 312, 773, 1480, 1703, 1989
AvaII	GGWCC	2 cut(s)	1945, 1979
BaeGI	GKGCMC	1 cut(s)	836
BaeI	ACNNNNGTAYC	2 cut(s)	1622, 1655
BalI	TGGCCA	1 cut(s)	1833
BamHI	GGATCC	2 cut(s)	30, 859
BanI	GGYRCC	3 cut(s)	831, 976, 1237
BanII	GRGCYC	4 cut(s)	267, 780, 902, 1865
BarI	GAAGNNNNNNTAC	2 cut(s)	275, 307
BbrPI	CACGTG	1 cut(s)	430
BbsI	GAAGAC	2 cut(s)	224, 309
Bbv12I	GWGCWC	4 cut(s)	267, 902, 968, 1936
BbvI	GCAGC	6 cut(s)	711, 801, 1069, 1153, 1405, 1613
BccI	CCATC	2 cut(s)	612, 1111
BceAI	ACGGC	1 cut(s)	411
BcgI	CGANNNNNNTGC	2 cut(s)	1074, 1108
BciT130I	CCWGG	1 cut(s)	215
BclI	TGATCA	2 cut(s)	598, 1509
BcoDI	GTCTC	3 cut(s)	71, 1271, 1397
BfaI	CTAG	2 cut(s)	1673, 1911
BfmI	CTRYAG	2 cut(s)	1015, 1083
BfuAI	ACCTGC	2 cut(s)	442, 1076
BglI	GCCNNNNNGGC	1 cut(s)	1493
BlpI	GCTNAGC	1 cut(s)	695
BmcAI	AGTACT	2 cut(s)	293, 1013
Bme18I	GGWCC	2 cut(s)	1945, 1979
BmeT110I	CYCGRG	6 cut(s)	19, 312, 773, 1480, 1703, 1989
BmgT120I	GGNCC	7 cut(s)	221, 384, 764, 1485, 1926, 1945, 1979
BmiI	GGNNCC	8 cut(s)	32, 491, 833, 861, 922, 978, 1239, 1614
BmrI	ACTGGG	2 cut(s)	774, 1077
BmsI	GCATC	2 cut(s)	147, 324
BmuI	ACTGGG	2 cut(s)	774, 1077
BpiI	GAAGAC	2 cut(s)	224, 309
BplI	GAGNNNNNCTC	2 cut(s)	1852, 1884
BpmI	CTGGAG	2 cut(s)	866, 1251
Bpu1102I	GCTNAGC	1 cut(s)	695
Bpu14I	TTCGAA	2 cut(s)	1190, 1325
BpuEI	CTTGAG	2 cut(s)	1266, 1367
BsaAI	YACGTR	1 cut(s)	430
BsaBI	GATNNNNATC	1 cut(s)	1466
BsaHI	GRCGYC	3 cut(s)	321, 1637, 1781
BsaI	GGTCTC	1 cut(s)	1397
BsaJI	CCNNGG	6 cut(s)	312, 387, 1101, 1481, 1704, 1785
BsaWI	WCCGGW	6 cut(s)	73, 235, 828, 862, 894, 1458
BsaXI	ACNNNNNCTCC	4 cut(s)	607, 637, 884, 914
Bsc4I	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 817, 947, 1752, 1840
Bse118I	RCCGGY	3 cut(s)	726, 1492, 1928
Bse1I	ACTGG	4 cut(s)	364, 780, 1072, 1982
Bse8I	GATNNNNATC	1 cut(s)	1466
BseAI	TCCGGA	2 cut(s)	73, 235
BseBI	CCWGG	1 cut(s)	215
BseDI	CCNNGG	6 cut(s)	312, 387, 1101, 1481, 1704, 1785
BseGI	GGATG	4 cut(s)	52, 339, 423, 1381
BseJI	GATNNNNATC	1 cut(s)	1466
BseLI	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 817, 947, 1752, 1840
BseMII	CTCAG	4 cut(s)	668, 686, 1490, 1686
BseNI	ACTGG	4 cut(s)	364, 780, 1072, 1982
BseRI	GAGGAG	2 cut(s)	1087, 1466
BseSI	GKGCMC	1 cut(s)	836
BseX3I	CGGCCG	1 cut(s)	1098
BseXI	GCAGC	6 cut(s)	711, 801, 1069, 1153, 1405, 1613
BseYI	CCCAGC	1 cut(s)	1604
BsgI	GTGCAG	1 cut(s)	1287
Bsh1236I	CGCG	4 cut(s)	478, 769, 1062, 1726
Bsh1285I	CGRYCG	4 cut(s)	246, 873, 895, 1101
BshFI	GGCC	9 cut(s)	218, 223, 386, 765, 1100, 1487, 1563, 1833, 1928
BshNI	GGYRCC	3 cut(s)	831, 976, 1237
BsiEI	CGRYCG	4 cut(s)	246, 873, 895, 1101
BsiHKAI	GWGCWC	4 cut(s)	267, 902, 968, 1936
BsiHKCI	CYCGRG	6 cut(s)	19, 312, 773, 1480, 1703, 1989
BslFI	GGGAC	2 cut(s)	329, 889
BslI	CCNNNNNNNGG	7 cut(s)	330, 370, 793, 817, 947, 1752, 1840
BsmAI	GTCTC	3 cut(s)	71, 1271, 1397
BsmBI	CGTCTC	1 cut(s)	71
BsmFI	GGGAC	2 cut(s)	329, 889
BsmI	GAATGC	3 cut(s)	1294, 1321, 1546
BsnI	GGCC	9 cut(s)	218, 223, 386, 765, 1100, 1487, 1563, 1833, 1928
Bso31I	GGTCTC	1 cut(s)	1397
BsoBI	CYCGRG	6 cut(s)	19, 312, 773, 1480, 1703, 1989
Bsp119I	TTCGAA	2 cut(s)	1190, 1325
Bsp1286I	GDGCHC	7 cut(s)	267, 780, 836, 902, 968, 1865, 1936
Bsp13I	TCCGGA	2 cut(s)	73, 235
Bsp1720I	GCTNAGC	1 cut(s)	695
Bsp19I	CCATGG	1 cut(s)	387
Bsp68I	TCGCGA	1 cut(s)	1062
BspANI	GGCC	9 cut(s)	218, 223, 386, 765, 1100, 1487, 1563, 1833, 1928
BspCNI	CTCAG	4 cut(s)	669, 687, 1491, 1687
BspEI	TCCGGA	2 cut(s)	73, 235
BspFNI	CGCG	4 cut(s)	478, 769, 1062, 1726
BspLI	GGNNCC	8 cut(s)	32, 491, 833, 861, 922, 978, 1239, 1614
BspMAI	CTGCAG	2 cut(s)	1019, 1087
BspMI	ACCTGC	2 cut(s)	442, 1076
BspPI	GGATC	7 cut(s)	25, 38, 205, 854, 867, 997, 1162
BspQI	GCTCTTC	1 cut(s)	956
BspT104I	TTCGAA	2 cut(s)	1190, 1325
BspT107I	GGYRCC	3 cut(s)	831, 976, 1237
BspTNI	GGTCTC	1 cut(s)	1397
BsrBI	CCGCTC	1 cut(s)	352
BsrFI	RCCGGY	3 cut(s)	726, 1492, 1928
BsrI	ACTGG	4 cut(s)	364, 780, 1072, 1982
BssAI	RCCGGY	3 cut(s)	726, 1492, 1928
BssECI	CCNNGG	6 cut(s)	312, 387, 1101, 1481, 1704, 1785
BssNI	GRCGYC	3 cut(s)	321, 1637, 1781
BssT1I	CCWWGG	2 cut(s)	387, 1785
Bst2UI	CCWGG	1 cut(s)	215
Bst4CI	ACNGT	4 cut(s)	247, 308, 1520, 1649
Bst6I	CTCTTC	3 cut(s)	201, 956, 989
BstACI	GRCGYC	3 cut(s)	321, 1637, 1781
BstAPI	GCANNNNNTGC	1 cut(s)	1550
BstBAI	YACGTR	1 cut(s)	430
BstBI	TTCGAA	2 cut(s)	1190, 1325
BstDEI	CTNAG	5 cut(s)	677, 695, 1499, 1695, 1895
BstDSI	CCRYGG	1 cut(s)	387
BstEII	GGTNACC	1 cut(s)	756
BstF5I	GGATG	4 cut(s)	52, 339, 423, 1381
BstFNI	CGCG	4 cut(s)	478, 769, 1062, 1726
BstHHI	GCGC	1 cut(s)	478
BstMAI	GTCTC	3 cut(s)	71, 1271, 1397
BstMCI	CGRYCG	4 cut(s)	246, 873, 895, 1101
BstNI	CCWGG	1 cut(s)	215
BstNSI	RCATGY	2 cut(s)	1418, 1881
BstPI	GGTNACC	1 cut(s)	756
BstSFI	CTRYAG	2 cut(s)	1015, 1083
BstSLI	GKGCMC	1 cut(s)	836
BstUI	CGCG	4 cut(s)	478, 769, 1062, 1726
BstV1I	GCAGC	6 cut(s)	711, 801, 1069, 1153, 1405, 1613
BstV2I	GAAGAC	2 cut(s)	224, 309
BstX2I	RGATCY	3 cut(s)	30, 210, 859
BstYI	RGATCY	3 cut(s)	30, 210, 859
BstZI	CGGCCG	1 cut(s)	1098
BsuRI	GGCC	9 cut(s)	218, 223, 386, 765, 1100, 1487, 1563, 1833, 1928
BtgI	CCRYGG	1 cut(s)	387
BtgZI	GCGATG	2 cut(s)	82, 124
BtsCI	GGATG	4 cut(s)	52, 339, 423, 1381
BtsI	GCAGTG	1 cut(s)	1024
BtsIMutI	CAGTG	4 cut(s)	989, 1024, 1065, 1654
BtuMI	TCGCGA	1 cut(s)	1062
BveI	ACCTGC	2 cut(s)	442, 1076
CfoI	GCGC	1 cut(s)	478
Cfr10I	RCCGGY	3 cut(s)	726, 1492, 1928
Cfr13I	GGNCC	7 cut(s)	221, 384, 764, 1485, 1926, 1945, 1979
Cfr9I	CCCGGG	1 cut(s)	312
CseI	GACGC	4 cut(s)	88, 329, 721, 1770
Csp6I	GTAC	6 cut(s)	292, 309, 593, 1012, 1077, 1456
CspCI	CAANNNNNGTGG	2 cut(s)	98, 133
CviAII	CATG	7 cut(s)	388, 602, 736, 1415, 1878, 1952, 1961
CviQI	GTAC	6 cut(s)	292, 309, 593, 1012, 1077, 1456
DdeI	CTNAG	5 cut(s)	677, 695, 1499, 1695, 1895
DraIII	CACNNNGTG	1 cut(s)	1840
EaeI	YGGCCR	2 cut(s)	1098, 1831
EagI	CGGCCG	1 cut(s)	1098
Eam1104I	CTCTTC	3 cut(s)	201, 956, 989
EarI	CTCTTC	3 cut(s)	201, 956, 989
EciI	GGCGGA	2 cut(s)	164, 1139
Ecl136II	GAGCTC	2 cut(s)	265, 900
EclXI	CGGCCG	1 cut(s)	1098
Eco130I	CCWWGG	2 cut(s)	387, 1785
Eco24I	GRGCYC	4 cut(s)	267, 780, 902, 1865
Eco31I	GGTCTC	1 cut(s)	1397
Eco32I	GATATC	1 cut(s)	840
Eco47I	GGWCC	2 cut(s)	1945, 1979
Eco52I	CGGCCG	1 cut(s)	1098
Eco53kI	GAGCTC	2 cut(s)	265, 900
Eco57I	CTGAAG	3 cut(s)	605, 725, 1013
Eco72I	CACGTG	1 cut(s)	430
Eco88I	CYCGRG	6 cut(s)	19, 312, 773, 1480, 1703, 1989
Eco91I	GGTNACC	1 cut(s)	756
EcoICRI	GAGCTC	2 cut(s)	265, 900
EcoO109I	RGGNCCY	1 cut(s)	1945
EcoO65I	GGTNACC	1 cut(s)	756
EcoRI	GAATTC	3 cut(s)	343, 1762, 1955
EcoRII	CCWGG	1 cut(s)	213
EcoRV	GATATC	1 cut(s)	840
EcoT14I	CCWWGG	2 cut(s)	387, 1785
EcoT22I	ATGCAT	2 cut(s)	140, 1546
EcoT38I	GRGCYC	4 cut(s)	267, 780, 902, 1865
ErhI	CCWWGG	2 cut(s)	387, 1785
Esp3I	CGTCTC	1 cut(s)	71
FaeI	CATG	7 cut(s)	391, 605, 739, 1418, 1881, 1955, 1964
FaqI	GGGAC	2 cut(s)	329, 889
FatI	CATG	7 cut(s)	387, 601, 735, 1414, 1877, 1951, 1960
FauI	CCCGC	5 cut(s)	345, 474, 507, 582, 774
FbaI	TGATCA	2 cut(s)	598, 1509
FblI	GTMKAC	1 cut(s)	564
FokI	GGATG	4 cut(s)	39, 346, 410, 1388
FriOI	GRGCYC	4 cut(s)	267, 780, 902, 1865
FspBI	CTAG	2 cut(s)	1673, 1911
GlaI	GCGC	1 cut(s)	477
GsaI	CCCAGC	1 cut(s)	1608
GsuI	CTGGAG	2 cut(s)	866, 1251
HaeIII	GGCC	9 cut(s)	218, 223, 386, 765, 1100, 1487, 1563, 1833, 1928
HgaI	GACGC	4 cut(s)	88, 329, 721, 1770
HhaI	GCGC	1 cut(s)	478
Hin1I	GRCGYC	3 cut(s)	321, 1637, 1781
Hin1II	CATG	7 cut(s)	391, 605, 739, 1418, 1881, 1955, 1964
Hin6I	GCGC	1 cut(s)	476
HinP1I	GCGC	1 cut(s)	476
HincII	GTYRAC	2 cut(s)	499, 565
HindII	GTYRAC	2 cut(s)	499, 565
HindIII	AAGCTT	1 cut(s)	10
HphI	GGTGA	7 cut(s)	97, 454, 475, 496, 652, 814, 877
Hpy166II	GTNNAC	4 cut(s)	499, 565, 751, 2017
Hpy8I	GTNNAC	4 cut(s)	499, 565, 751, 2017
Hpy99I	CGWCG	5 cut(s)	30, 323, 397, 524, 1056
HpyAV	CCTTC	6 cut(s)	277, 439, 628, 700, 1875, 1895
HpyCH4III	ACNGT	4 cut(s)	247, 308, 1520, 1649
HpyCH4IV	ACGT	3 cut(s)	429, 1637, 2019
HpyF3I	CTNAG	5 cut(s)	677, 695, 1499, 1695, 1895
HpySE526I	ACGT	3 cut(s)	429, 1637, 2019
Hsp92I	GRCGYC	3 cut(s)	321, 1637, 1781
Hsp92II	CATG	7 cut(s)	391, 605, 739, 1418, 1881, 1955, 1964
HspAI	GCGC	1 cut(s)	476
Kpn2I	TCCGGA	2 cut(s)	73, 235
Ksp22I	TGATCA	2 cut(s)	598, 1509
LguI	GCTCTTC	1 cut(s)	956
Lsp1109I	GCAGC	6 cut(s)	711, 801, 1069, 1153, 1405, 1613
LweI	GCATC	2 cut(s)	147, 324
MaeI	CTAG	2 cut(s)	1673, 1911
MaeII	ACGT	3 cut(s)	429, 1637, 2019
MaeIII	GTNAC	5 cut(s)	756, 865, 881, 1660, 1984
MbiI	CCGCTC	1 cut(s)	352
MflI	RGATCY	3 cut(s)	30, 210, 859
MhlI	GDGCHC	7 cut(s)	267, 780, 836, 902, 968, 1865, 1936
MlsI	TGGCCA	1 cut(s)	1833
MluCI	AATT	7 cut(s)	343, 458, 660, 982, 1719, 1762, 1955
MluNI	TGGCCA	1 cut(s)	1833
MlyI	GAGTC	1 cut(s)	1273
MmeI	TCCRAC	2 cut(s)	8, 1034
Mox20I	TGGCCA	1 cut(s)	1833
Mph1103I	ATGCAT	2 cut(s)	140, 1546
MroI	TCCGGA	2 cut(s)	73, 235
MroXI	GAANNNNTTC	2 cut(s)	365, 914
MscI	TGGCCA	1 cut(s)	1833
MseI	TTAA	4 cut(s)	14, 531, 1206, 1857
MslI	CAYNNNNRTG	2 cut(s)	428, 971
Msp20I	TGGCCA	1 cut(s)	1833
MspA1I	CMGCKG	2 cut(s)	1082, 1604
Mva1269I	GAATGC	3 cut(s)	1294, 1321, 1546
MvaI	CCWGG	1 cut(s)	215
MvnI	CGCG	4 cut(s)	478, 769, 1062, 1726
NcoI	CCATGG	1 cut(s)	387
NlaIII	CATG	7 cut(s)	391, 605, 739, 1418, 1881, 1955, 1964
NlaIV	GGNNCC	8 cut(s)	32, 491, 833, 861, 922, 978, 1239, 1614
NmeAIII	GCCGAG	2 cut(s)	1073, 1126
NmuCI	GTSAC	4 cut(s)	865, 881, 1660, 1984
NruI	TCGCGA	1 cut(s)	1062
NsiI	ATGCAT	2 cut(s)	140, 1546
NspI	RCATGY	2 cut(s)	1418, 1881
NspV	TTCGAA	2 cut(s)	1190, 1325
OliI	CACNNNNGTG	1 cut(s)	971
PaeI	GCATGC	1 cut(s)	1418
PciSI	GCTCTTC	1 cut(s)	956
PctI	GAATGC	3 cut(s)	1294, 1321, 1546
PdmI	GAANNNNTTC	2 cut(s)	365, 914
PflMI	CCANNNNNTGG	1 cut(s)	1840
Ple19I	CGATCG	1 cut(s)	873
PleI	GAGTC	1 cut(s)	1273
PmaCI	CACGTG	1 cut(s)	430
PmlI	CACGTG	1 cut(s)	430
PpsI	GAGTC	1 cut(s)	1273
Ppu21I	YACGTR	1 cut(s)	430
PpuMI	RGGWCCY	1 cut(s)	1945
Psp124BI	GAGCTC	2 cut(s)	267, 902
Psp5II	RGGWCCY	1 cut(s)	1945
Psp6I	CCWGG	1 cut(s)	213
PspCI	CACGTG	1 cut(s)	430
PspEI	GGTNACC	1 cut(s)	756
PspFI	CCCAGC	1 cut(s)	1604
PspGI	CCWGG	1 cut(s)	213
PspN4I	GGNNCC	8 cut(s)	32, 491, 833, 861, 922, 978, 1239, 1614
PspPI	GGNCC	7 cut(s)	221, 384, 764, 1485, 1926, 1945, 1979
PspPPI	RGGWCCY	1 cut(s)	1945
PstI	CTGCAG	2 cut(s)	1019, 1087
PsuI	RGATCY	3 cut(s)	30, 210, 859
PvuI	CGATCG	1 cut(s)	873
PvuII	CAGCTG	2 cut(s)	1082, 1604
RruI	TCGCGA	1 cut(s)	1062
RsaI	GTAC	6 cut(s)	293, 310, 594, 1013, 1078, 1457
RsaNI	GTAC	6 cut(s)	292, 309, 593, 1012, 1077, 1456
RseI	CAYNNNNRTG	2 cut(s)	428, 971
SacI	GAGCTC	2 cut(s)	267, 902
SalI	GTCGAC	1 cut(s)	563
SapI	GCTCTTC	1 cut(s)	956
SaqAI	TTAA	4 cut(s)	14, 531, 1206, 1857
Sau96I	GGNCC	7 cut(s)	221, 384, 764, 1485, 1926, 1945, 1979
ScaI	AGTACT	2 cut(s)	293, 1013
SchI	GAGTC	1 cut(s)	1273
SduI	GDGCHC	7 cut(s)	267, 780, 836, 902, 968, 1865, 1936
SfaNI	GCATC	2 cut(s)	147, 324
SfcI	CTRYAG	2 cut(s)	1015, 1083
SfuI	TTCGAA	2 cut(s)	1190, 1325
SinI	GGWCC	2 cut(s)	1945, 1979
SmaI	CCCGGG	1 cut(s)	314
SmiMI	CAYNNNNRTG	2 cut(s)	428, 971
SmlI	CTYRAG	2 cut(s)	1281, 1382
SmoI	CTYRAG	2 cut(s)	1281, 1382
SphI	GCATGC	1 cut(s)	1418
Sse9I	AATT	7 cut(s)	343, 458, 660, 982, 1719, 1762, 1955
SspMI	CTAG	2 cut(s)	1673, 1911
SstI	GAGCTC	2 cut(s)	267, 902
StyI	CCWWGG	2 cut(s)	387, 1785
TaaI	ACNGT	4 cut(s)	247, 308, 1520, 1649
TaiI	ACGT	3 cut(s)	432, 1640, 2022
TasI	AATT	7 cut(s)	343, 458, 660, 982, 1719, 1762, 1955
TatI	WGTACW	3 cut(s)	291, 1011, 1076
TauI	GCSGC	5 cut(s)	226, 481, 789, 1100, 1243
Tru1I	TTAA	4 cut(s)	14, 531, 1206, 1857
Tru9I	TTAA	4 cut(s)	14, 531, 1206, 1857
TscAI	CASTG	4 cut(s)	996, 1024, 1072, 1654
TseFI	GTSAC	4 cut(s)	865, 881, 1660, 1984
TseI	GCWGC	6 cut(s)	699, 789, 1082, 1166, 1418, 1601
Tsp45I	GTSAC	4 cut(s)	865, 881, 1660, 1984
TspDTI	ATGAA	7 cut(s)	41, 356, 923, 974, 1127, 1968, 1977
TspGWI	ACGGA	2 cut(s)	872, 1023
TspMI	CCCGGG	1 cut(s)	312
TspRI	CASTG	4 cut(s)	996, 1024, 1072, 1654
Van91I	CCANNNNNTGG	1 cut(s)	1840
VpaK11BI	GGWCC	2 cut(s)	1945, 1979
XapI	RAATTY	3 cut(s)	343, 1762, 1955
XbaI	TCTAGA	1 cut(s)	1910
XceI	RCATGY	2 cut(s)	1418, 1881
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1418
XmaI	CCCGGG	1 cut(s)	312
XmiI	GTMKAC	1 cut(s)	564
XmnI	GAANNNNTTC	2 cut(s)	365, 914
XspI	CTAG	2 cut(s)	1673, 1911
ZraI	GACGTC	1 cut(s)	1638
ZrmI	AGTACT	2 cut(s)	293, 1013
Zsp2I	ATGCAT	2 cut(s)	140, 1546

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.