Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003359

Physical Location & Seq

Genomic Coordinates Reverse (-)

27579870 .. 27581927

Gene Structure 2058 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1183300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGAATTGGAGATTTGATAGCGACGACCGCCGGATACTCATGTTTCCGGCGGTGCGCCCTTGCGAATGTATTTCTCCGGAGACCCTTCTCGACTCTCTGATCCCTCTCTCCCACACAATCTGCAATTTCCACTCGAAATGCTTCGCGACGCAACGGAAGAATAGCCGGGAAGCAATTCGCCTAGTTCGTGTTCTTCTCATGTTCTTTGAGGAGGCATGCGAGTGCAATTCGGCTCTTTCGAAATCTTCCGCTGTCCTCTGCTTCTCCGAGCTTCATTTCGTCTTCCAAAAAATCCGGTTTTTGTTGGAAGACTGCACGAGAGAAGGCGGTCGGATTTTGATGCTGACGAAATGCGAGTTTCTTGCTACCCAATTTCGGCTGCTGATTCGAGCTTTGGCGACGGCTCTTGATGTTTTTCCGTTGAAATTGATCGATGTTGGCGATGAAGTTAAGGAATTGGTTGAATTGGTGTCGAAGCAATCGTGGAAGGTCAAAATCGAGCTCGAAGCGGATGACGAATTGGCTTCGAAACGATTGCTTGCGATTCTGAGCCAATTCGAAAGGGGAATCGAGCCCGATTTGAATGCCGTAAAGCGGGTCCTCGATTACCTTCGAGTCAAAAGATGGAGCGACTGCAACATGGAGATCAAATTCTTGGAAGAAGAAATCGCTTTCAAGCGTTCCGATCGCGACGAGAGGGAGGTTCCTTTTCTCAGTAGCTTGGTGGGGTTCCTGAGCTACAGCAGGGGAGTGATCTTCGAAACATTGGATCATCAAAACGCTGCGAATCAAATCGATTCCAGGTGCAACGTGGAGATGTTTCGTTGCTTAAACCCTGAAGATTTCAAGTGCCCGATTTCTCTTGAGCTAATGCTTGATCCGGTGACTGTATCTACAGGTCAGACATACGATCGAAGTTCGATTCAGAAATGGCTCCGCGCCGGAAACATGCTCTGCCCCAAAACAGGAGAGGTGATCACAAACACAGAGTTGGTACCGAATTCGATTCTTCGCAAGCTACTAAAAAATCTTTGTGAATACAATGGCGTTTCTCTTGGCAGGCCAGGAACGCAAAGCTCCGACAAAGATCAAACGGTCGCGCATGGTAGCCCCGTTGCTAAACAAGCAATGAAGTTTCTCTCGAGATTTCTCAGCGGAAGGGTTGCTTGCGGAACAAGCGAACAGAAAAACAAGGCTGCTTATGAGATTCGGCTGCTAGCCAAATCAAACATTTTCAATAGGTCTTGTTTTATCAAGGCTGGCTCCATCCCTCCTCTGCTGAAACTACTGAATTCCGCGGATGCATCCACGCAAGTGAATGCAATCGCAGCCTTGTTGAAGCTCGCGAAATACGCAAGCGGAATGAAATTGATAATGTCATGGGGAGGACTACCATCAGTTGTTGCTGTTCTTAAAAATGGGTTGAGTTTAGAAGCCCGGCAATGCGCAGCTGCAACGATATTTCACCTCTCTTTGACAAGCCAGTATCGAAAACTAATTGGAGAATTACCTGAGGCCATCCCAGCTCTTGTGGAGCTGATCAGGGAAGGAACAACGTGTGGCAAAAAGAACTCCGTGGTTGCAATTCTCGGGCTGCTCCTTCGTGCCAAGAATCTACAAAAAGTGCTGGACGCAGGCATTGTTCCATTGCTAATTGACATAATATCTTCATCATCAGAAAACCCTGATCAGCTTGTTGATGATGCATTAGCAATTCTCGCAATTTTGGCCGAGAATGCGGTAGGATCACATGAAATCCTCTACGCCATTGATCTGCATTTGATCATCCGCATTCTTCAAACTTCGACATCCCAAAGAGCGAAAAAGTATTGTGTTTCAACATTGCTGTCTTTGTGCATCAATGGCGGGGCGGAAGTTGTTGCTGTTTTGGCGACGGAACCATCTCTAATGCCTAGACTGTTTTCGCTCTTGACTGATGGAACATCTCACTCGATCAATGAAGCGTCCTCCCTCATCAAATTTCTCCATAACTTCGGTGAAACTAGCGCACCGAGATGGATGAGTACTGCAGTTCGGCGCAAACAAAGCGTTCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0010029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

686

Amino Acids

76.19

Weight (kDa)

8.61

Isoelectric Point (pI)

42.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	53 - 160	6.7e-10	PUB10-like, N-terminal domain
U-box	PF04564	279 - 340	6.5e-16	U-box domain
ARM_PUB	PF25598	395 - 662	1.1e-11	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10560 AT1G60190
fragaria_vesca	FvH4_4g34620 FvH4_5g05140
malus_domestica	MD06G1177100.v1.1 MD13G1090900.v1.1 MD14G1183300.v1.1 MD16G1091300.v1.1
prunus_persica	Prupe.1G255000_v2.0.a1 Prupe.5G175800_v2.0.a1
pyrus_communis	pycom06g15720 pycom13g07890 pycom14g15220 pycom16g07800
rosa_chinensis	RchiOBHm_Chr4g0443981 RchiOBHm_Chr7g0193831
rosa_laevigata	RLG00000004242 RLG00000005870
rosa_multiflora	Rmu_co8252843.1_g000001 Rmu_sc0001436.1_g000032 Rmu_sc0027702.1_g000002 Rmu_sc0029481.1_g000002
rosa_roxburghii	Rroxscaffold_3G00261790 Rroxscaffold_5G00384530
rosa_rugosa	Rorug04G0348700 Rorug07G0012400
rosa_samantha	Rh4AG409400 Rh4BG420500 Rh4CG434500 Rh4DG415500 Rh7AG139000 Rh7BG139200 Rh7CG142900 Rh7DG140500
rosa_wichuraiana	Rw4G035170 Rw7G011850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1447
Acc65I	GGTACC	1 cut(s)	994
AccB1I	GGYRCC	1 cut(s)	994
AccII	CGCG	6 cut(s)	146, 690, 939, 1100, 1298, 1346
AccIII	TCCGGA	1 cut(s)	76
AclWI	GGATC	4 cut(s)	94, 777, 872, 1753
AcoI	YGGCCR	1 cut(s)	1728
AcsI	RAATTY	4 cut(s)	650, 1000, 1291, 1979
AcuI	CTGAAG	1 cut(s)	858
AfaI	GTAC	2 cut(s)	996, 2026
AfiI	CCNNNNNNNGG	3 cut(s)	375, 594, 965
AflIII	ACRYGT	1 cut(s)	1556
AgsI	TTSAA	9 cut(s)	424, 464, 583, 676, 847, 1237, 1339, 1799, 1839
AjnI	CCWGG	2 cut(s)	800, 1063
AleI	CACNNNNGTG	1 cut(s)	1313
Alw21I	GWGCWC	1 cut(s)	504
Alw26I	GTCTC	1 cut(s)	74
AlwI	GGATC	4 cut(s)	94, 777, 872, 1753
Ama87I	CYCGRG	2 cut(s)	1141, 1589
Aor13HI	TCCGGA	1 cut(s)	76
AoxI	GGCC	3 cut(s)	1061, 1515, 1728
ApeKI	GCWGC	8 cut(s)	379, 782, 1196, 1213, 1328, 1448, 1451, 1594
ApoI	RAATTY	4 cut(s)	650, 1000, 1291, 1979
Asp700I	GAANNNNTTC	2 cut(s)	140, 174
Asp718I	GGTACC	1 cut(s)	994
AspLEI	GCGC	6 cut(s)	57, 941, 1102, 1448, 2009, 2040
AspS9I	GGNCC	1 cut(s)	598
AsuC2I	CCSGG	2 cut(s)	167, 1438
AsuHPI	GGTGA	4 cut(s)	895, 985, 1457, 2009
AsuII	TTCGAA	4 cut(s)	239, 527, 558, 759
AsuNHI	GCTAGC	1 cut(s)	1216
AvaI	CYCGRG	2 cut(s)	1141, 1589
AvaII	GGWCC	1 cut(s)	598
AxyI	CCTNAGG	1 cut(s)	1512
BaeGI	GKGCMC	1 cut(s)	854
BaeI	ACNNNNGTAYC	2 cut(s)	26, 59
BanI	GGYRCC	1 cut(s)	994
BanII	GRGCYC	2 cut(s)	504, 576
BauI	CACGAG	1 cut(s)	316
BbsI	GAAGAC	2 cut(s)	274, 315
Bbv12I	GWGCWC	1 cut(s)	504
BbvI	GCAGC	8 cut(s)	366, 769, 1183, 1200, 1340, 1438, 1460, 1581
BccI	CCATC	7 cut(s)	618, 1274, 1402, 1526, 1909, 1931, 2010
BceAI	ACGGC	2 cut(s)	417, 572
BcgI	CGANNNNNNTGC	6 cut(s)	344, 378, 517, 551, 566, 600
BciT130I	CCWGG	2 cut(s)	802, 1065
BciVI	GTATCC	1 cut(s)	27
BclI	TGATCA	4 cut(s)	975, 1539, 1687, 1782
BcnI	CCSGG	2 cut(s)	167, 1438
BcoDI	GTCTC	1 cut(s)	74
BfaI	CTAG	4 cut(s)	182, 1217, 1914, 2004
BfmI	CTRYAG	3 cut(s)	739, 894, 2028
BfuI	GTATCC	1 cut(s)	27
BisI	GCNGC	8 cut(s)	380, 783, 1197, 1214, 1329, 1449, 1452, 1595
BlsI	GCNGC	8 cut(s)	381, 784, 1198, 1215, 1330, 1450, 1453, 1596
BmcAI	AGTACT	1 cut(s)	2026
Bme1390I	CCNGG	4 cut(s)	167, 802, 1065, 1438
Bme18I	GGWCC	1 cut(s)	598
BmeT110I	CYCGRG	2 cut(s)	1141, 1589
BmgT120I	GGNCC	1 cut(s)	598
BmiI	GGNNCC	7 cut(s)	599, 705, 731, 935, 996, 1264, 1899
BmrFI	CCNGG	4 cut(s)	167, 802, 1065, 1438
BmsI	GCATC	5 cut(s)	330, 1291, 1313, 1693, 1866
BmtI	GCTAGC	1 cut(s)	1220
BpiI	GAAGAC	2 cut(s)	274, 315
Bpu10I	CCTNAGC	1 cut(s)	734
Bpu14I	TTCGAA	4 cut(s)	239, 527, 558, 759
BpuEI	CTTGAG	1 cut(s)	884
BpuMI	CCSGG	2 cut(s)	167, 1438
Bsa29I	ATCGAT	2 cut(s)	432, 795
BsaI	GGTCTC	1 cut(s)	74
BsaJI	CCNNGG	2 cut(s)	1296, 1575
BsaWI	WCCGGW	3 cut(s)	76, 294, 880
Bsc4I	CCNNNNNNNGG	3 cut(s)	375, 594, 965
Bse1I	ACTGG	1 cut(s)	1483
Bse21I	CCTNAGG	1 cut(s)	1512
Bse3DI	GCAATG	4 cut(s)	1134, 1448, 1646, 1841
BseAI	TCCGGA	1 cut(s)	76
BseBI	CCWGG	2 cut(s)	802, 1065
BseCI	ATCGAT	2 cut(s)	432, 795
BseDI	CCNNGG	2 cut(s)	1296, 1575
BseGI	GGATG	8 cut(s)	517, 1266, 1304, 1306, 1518, 1785, 1808, 2025
BseLI	CCNNNNNNNGG	3 cut(s)	375, 594, 965
BseMI	GCAATG	4 cut(s)	1134, 1448, 1646, 1841
BseMII	CTCAG	5 cut(s)	539, 725, 727, 1165, 1503
BseNI	ACTGG	1 cut(s)	1483
BseRI	GAGGAG	2 cut(s)	224, 1263
BseSI	GKGCMC	1 cut(s)	854
BseXI	GCAGC	8 cut(s)	366, 769, 1183, 1200, 1340, 1438, 1460, 1581
BseYI	CCCAGC	1 cut(s)	1522
BsgI	GTGCAG	1 cut(s)	298
Bsh1236I	CGCG	6 cut(s)	146, 690, 939, 1100, 1298, 1346
Bsh1285I	CGRYCG	5 cut(s)	28, 331, 688, 913, 1098
BshFI	GGCC	3 cut(s)	1063, 1517, 1730
BshNI	GGYRCC	1 cut(s)	994
BshVI	ATCGAT	2 cut(s)	432, 795
BsiEI	CGRYCG	5 cut(s)	28, 331, 688, 913, 1098
BsiHKAI	GWGCWC	1 cut(s)	504
BsiHKCI	CYCGRG	2 cut(s)	1141, 1589
BsiSI	CCGG	8 cut(s)	31, 47, 77, 166, 295, 881, 942, 1438
BslI	CCNNNNNNNGG	3 cut(s)	375, 594, 965
BsmAI	GTCTC	1 cut(s)	74
BsmI	GAATGC	4 cut(s)	589, 1324, 1741, 1791
BsnI	GGCC	3 cut(s)	1063, 1517, 1730
Bso31I	GGTCTC	1 cut(s)	74
BsoBI	CYCGRG	2 cut(s)	1141, 1589
Bsp119I	TTCGAA	4 cut(s)	239, 527, 558, 759
Bsp1286I	GDGCHC	3 cut(s)	504, 576, 854
Bsp13I	TCCGGA	1 cut(s)	76
Bsp68I	TCGCGA	3 cut(s)	146, 690, 1346
BspANI	GGCC	3 cut(s)	1063, 1517, 1730
BspCNI	CTCAG	5 cut(s)	540, 726, 726, 1164, 1504
BspDI	ATCGAT	2 cut(s)	432, 795
BspEI	TCCGGA	1 cut(s)	76
BspFNI	CGCG	6 cut(s)	146, 690, 939, 1100, 1298, 1346
BspLI	GGNNCC	7 cut(s)	599, 705, 731, 935, 996, 1264, 1899
BspMAI	CTGCAG	1 cut(s)	2032
BspOI	GCTAGC	1 cut(s)	1220
BspPI	GGATC	4 cut(s)	94, 777, 872, 1753
BspT104I	TTCGAA	4 cut(s)	239, 527, 558, 759
BspT107I	GGYRCC	1 cut(s)	994
BspTNI	GGTCTC	1 cut(s)	74
BsrDI	GCAATG	4 cut(s)	1134, 1448, 1646, 1841
BsrI	ACTGG	1 cut(s)	1483
BssECI	CCNNGG	2 cut(s)	1296, 1575
BssSI	CACGAG	1 cut(s)	316
Bst2BI	CACGAG	1 cut(s)	316
Bst2UI	CCWGG	2 cut(s)	802, 1065
Bst4CI	ACNGT	3 cut(s)	889, 1096, 1920
BstBI	TTCGAA	4 cut(s)	239, 527, 558, 759
BstDEI	CTNAG	5 cut(s)	548, 713, 734, 1151, 1512
BstDSI	CCRYGG	2 cut(s)	1296, 1575
BstF5I	GGATG	8 cut(s)	517, 1266, 1304, 1306, 1518, 1785, 1808, 2025
BstFNI	CGCG	6 cut(s)	146, 690, 939, 1100, 1298, 1346
BstHHI	GCGC	6 cut(s)	57, 941, 1102, 1448, 2009, 2040
BstMAI	GTCTC	1 cut(s)	74
BstMCI	CGRYCG	5 cut(s)	28, 331, 688, 913, 1098
BstNI	CCWGG	2 cut(s)	802, 1065
BstNSI	RCATGY	2 cut(s)	219, 952
BstSCI	CCNGG	4 cut(s)	165, 800, 1063, 1436
BstSFI	CTRYAG	3 cut(s)	739, 894, 2028
BstSLI	GKGCMC	1 cut(s)	854
BstUI	CGCG	6 cut(s)	146, 690, 939, 1100, 1298, 1346
BstV1I	GCAGC	8 cut(s)	366, 769, 1183, 1200, 1340, 1438, 1460, 1581
BstV2I	GAAGAC	2 cut(s)	274, 315
Bsu15I	ATCGAT	2 cut(s)	432, 795
Bsu36I	CCTNAGG	1 cut(s)	1512
BsuI	GTATCC	1 cut(s)	27
BsuRI	GGCC	3 cut(s)	1063, 1517, 1730
BsuTUI	ATCGAT	2 cut(s)	432, 795
BtgI	CCRYGG	2 cut(s)	1296, 1575
BtgZI	GCGATG	1 cut(s)	456
BtsCI	GGATG	8 cut(s)	517, 1266, 1304, 1306, 1518, 1785, 1808, 2025
BtuMI	TCGCGA	3 cut(s)	146, 690, 1346
CfoI	GCGC	6 cut(s)	57, 941, 1102, 1448, 2009, 2040
Cfr13I	GGNCC	1 cut(s)	598
Cfr42I	CCGCGG	1 cut(s)	1299
ClaI	ATCGAT	2 cut(s)	432, 795
CseI	GACGC	3 cut(s)	157, 1640, 1953
Csp6I	GTAC	2 cut(s)	995, 2025
CviAII	CATG	8 cut(s)	40, 199, 216, 640, 949, 1103, 1380, 1751
CviQI	GTAC	2 cut(s)	995, 2025
DdeI	CTNAG	5 cut(s)	548, 713, 734, 1151, 1512
EaeI	YGGCCR	1 cut(s)	1728
EciI	GGCGGA	1 cut(s)	1886
Ecl136II	GAGCTC	1 cut(s)	502
Eco24I	GRGCYC	2 cut(s)	504, 576
Eco31I	GGTCTC	1 cut(s)	74
Eco47I	GGWCC	1 cut(s)	598
Eco53kI	GAGCTC	1 cut(s)	502
Eco57I	CTGAAG	1 cut(s)	858
Eco81I	CCTNAGG	1 cut(s)	1512
Eco88I	CYCGRG	2 cut(s)	1141, 1589
EcoICRI	GAGCTC	1 cut(s)	502
EcoO109I	RGGNCCY	1 cut(s)	598
EcoRI	GAATTC	2 cut(s)	1000, 1291
EcoRII	CCWGG	2 cut(s)	800, 1063
EcoT22I	ATGCAT	2 cut(s)	1306, 1708
EcoT38I	GRGCYC	2 cut(s)	504, 576
FaeI	CATG	8 cut(s)	43, 202, 219, 643, 952, 1106, 1383, 1754
FalI	AAGNNNNNCTT	2 cut(s)	1150, 1182
FatI	CATG	8 cut(s)	39, 198, 215, 639, 948, 1102, 1379, 1750
FauI	CCCGC	2 cut(s)	588, 1859
FbaI	TGATCA	4 cut(s)	975, 1539, 1687, 1782
Fnu4HI	GCNGC	8 cut(s)	380, 783, 1197, 1214, 1329, 1449, 1452, 1595
FokI	GGATG	8 cut(s)	524, 1253, 1291, 1313, 1505, 1772, 1795, 2032
FriOI	GRGCYC	2 cut(s)	504, 576
Fsp4HI	GCNGC	8 cut(s)	380, 783, 1197, 1214, 1329, 1449, 1452, 1595
FspBI	CTAG	4 cut(s)	182, 1217, 1914, 2004
FspI	TGCGCA	1 cut(s)	1447
GlaI	GCGC	6 cut(s)	56, 940, 1101, 1447, 2008, 2039
GluI	GCNGC	8 cut(s)	380, 783, 1197, 1214, 1329, 1449, 1452, 1595
GsaI	CCCAGC	1 cut(s)	1526
HaeIII	GGCC	3 cut(s)	1063, 1517, 1730
HapII	CCGG	8 cut(s)	31, 47, 77, 166, 295, 881, 942, 1438
HgaI	GACGC	3 cut(s)	157, 1640, 1953
HhaI	GCGC	6 cut(s)	57, 941, 1102, 1448, 2009, 2040
Hin1II	CATG	8 cut(s)	43, 202, 219, 643, 952, 1106, 1383, 1754
Hin6I	GCGC	6 cut(s)	55, 939, 1100, 1446, 2007, 2038
HinP1I	GCGC	6 cut(s)	55, 939, 1100, 1446, 2007, 2038
HpaII	CCGG	8 cut(s)	31, 47, 77, 166, 295, 881, 942, 1438
HphI	GGTGA	4 cut(s)	895, 985, 1457, 2009
Hpy188I	TCNGA	9 cut(s)	99, 268, 333, 549, 685, 903, 927, 1081, 1678
Hpy99I	CGWCG	5 cut(s)	26, 151, 403, 695, 1897
HpyAV	CCTTC	7 cut(s)	95, 317, 481, 620, 1152, 1541, 1610
HpyCH4III	ACNGT	3 cut(s)	889, 1096, 1920
HpyCH4IV	ACGT	2 cut(s)	810, 1556
HpyF3I	CTNAG	5 cut(s)	548, 713, 734, 1151, 1512
HpySE526I	ACGT	2 cut(s)	810, 1556
Hsp92II	CATG	8 cut(s)	43, 202, 219, 643, 952, 1106, 1383, 1754
HspAI	GCGC	6 cut(s)	55, 939, 1100, 1446, 2007, 2038
Kpn2I	TCCGGA	1 cut(s)	76
KpnI	GGTACC	1 cut(s)	998
Ksp22I	TGATCA	4 cut(s)	975, 1539, 1687, 1782
KspI	CCGCGG	1 cut(s)	1299
LmnI	GCTCC	6 cut(s)	627, 939, 1082, 1268, 1534, 1602
Lsp1109I	GCAGC	8 cut(s)	366, 769, 1183, 1200, 1340, 1438, 1460, 1581
LweI	GCATC	5 cut(s)	330, 1291, 1313, 1693, 1866
MaeI	CTAG	4 cut(s)	182, 1217, 1914, 2004
MaeII	ACGT	2 cut(s)	810, 1556
MaeIII	GTNAC	1 cut(s)	883
MhlI	GDGCHC	3 cut(s)	504, 576, 854
MlyI	GAGTC	2 cut(s)	86, 624
MmeI	TCCRAC	3 cut(s)	285, 311, 1104
Mph1103I	ATGCAT	2 cut(s)	1306, 1708
MroI	TCCGGA	1 cut(s)	76
MroXI	GAANNNNTTC	2 cut(s)	140, 174
MseI	TTAA	3 cut(s)	450, 830, 1413
MslI	CAYNNNNRTG	3 cut(s)	220, 1313, 2014
MspA1I	CMGCKG	4 cut(s)	251, 1155, 1298, 1451
MspI	CCGG	8 cut(s)	31, 47, 77, 166, 295, 881, 942, 1438
MspR9I	CCNGG	4 cut(s)	167, 802, 1065, 1438
Mva1269I	GAATGC	4 cut(s)	589, 1324, 1741, 1791
MvaI	CCWGG	2 cut(s)	802, 1065
MvnI	CGCG	6 cut(s)	146, 690, 939, 1100, 1298, 1346
NciI	CCSGG	2 cut(s)	167, 1438
NheI	GCTAGC	1 cut(s)	1216
NlaIII	CATG	8 cut(s)	43, 202, 219, 643, 952, 1106, 1383, 1754
NlaIV	GGNNCC	7 cut(s)	599, 705, 731, 935, 996, 1264, 1899
NmeAIII	GCCGAG	1 cut(s)	1756
NmuCI	GTSAC	1 cut(s)	883
NruI	TCGCGA	3 cut(s)	146, 690, 1346
NsbI	TGCGCA	1 cut(s)	1447
NsiI	ATGCAT	2 cut(s)	1306, 1708
NspI	RCATGY	2 cut(s)	219, 952
NspV	TTCGAA	4 cut(s)	239, 527, 558, 759
OliI	CACNNNNGTG	1 cut(s)	1313
PaeI	GCATGC	1 cut(s)	219
PaeR7I	CTCGAG	1 cut(s)	1141
PcsI	WCGNNNNNNNCGW	4 cut(s)	184, 236, 438, 479
PctI	GAATGC	4 cut(s)	589, 1324, 1741, 1791
PdmI	GAANNNNTTC	2 cut(s)	140, 174
PfeI	GAWTC	9 cut(s)	385, 544, 567, 787, 797, 922, 1006, 1207, 1612
PkrI	GCNGC	8 cut(s)	381, 784, 1198, 1215, 1330, 1450, 1453, 1596
Ple19I	CGATCG	2 cut(s)	688, 913
PleI	GAGTC	2 cut(s)	86, 623
PpsI	GAGTC	2 cut(s)	86, 623
PpuMI	RGGWCCY	1 cut(s)	598
Psp124BI	GAGCTC	1 cut(s)	504
Psp5II	RGGWCCY	1 cut(s)	598
Psp6I	CCWGG	2 cut(s)	800, 1063
PspFI	CCCAGC	1 cut(s)	1522
PspGI	CCWGG	2 cut(s)	800, 1063
PspN4I	GGNNCC	7 cut(s)	599, 705, 731, 935, 996, 1264, 1899
PspPI	GGNCC	1 cut(s)	598
PspPPI	RGGWCCY	1 cut(s)	598
PstI	CTGCAG	1 cut(s)	2032
PvuI	CGATCG	2 cut(s)	688, 913
PvuII	CAGCTG	1 cut(s)	1451
RruI	TCGCGA	3 cut(s)	146, 690, 1346
RsaI	GTAC	2 cut(s)	996, 2026
RsaNI	GTAC	2 cut(s)	995, 2025
RseI	CAYNNNNRTG	3 cut(s)	220, 1313, 2014
SacI	GAGCTC	1 cut(s)	504
SacII	CCGCGG	1 cut(s)	1299
SaqAI	TTAA	3 cut(s)	450, 830, 1413
SatI	GCNGC	8 cut(s)	380, 783, 1197, 1214, 1329, 1449, 1452, 1595
Sau96I	GGNCC	1 cut(s)	598
ScaI	AGTACT	1 cut(s)	2026
SchI	GAGTC	2 cut(s)	86, 624
ScrFI	CCNGG	4 cut(s)	167, 802, 1065, 1438
SduI	GDGCHC	3 cut(s)	504, 576, 854
SfaNI	GCATC	5 cut(s)	330, 1291, 1313, 1693, 1866
SfcI	CTRYAG	3 cut(s)	739, 894, 2028
Sfr274I	CTCGAG	1 cut(s)	1141
Sfr303I	CCGCGG	1 cut(s)	1299
SfuI	TTCGAA	4 cut(s)	239, 527, 558, 759
SgrBI	CCGCGG	1 cut(s)	1299
SinI	GGWCC	1 cut(s)	598
SlaI	CTCGAG	1 cut(s)	1141
SmiMI	CAYNNNNRTG	3 cut(s)	220, 1313, 2014
SmlI	CTYRAG	2 cut(s)	863, 1141
SmoI	CTYRAG	2 cut(s)	863, 1141
SphI	GCATGC	1 cut(s)	219
SspMI	CTAG	4 cut(s)	182, 1217, 1914, 2004
SstI	GAGCTC	1 cut(s)	504
StyD4I	CCNGG	4 cut(s)	165, 800, 1063, 1436
TaaI	ACNGT	3 cut(s)	889, 1096, 1920
TaiI	ACGT	2 cut(s)	813, 1559
TatI	WGTACW	1 cut(s)	2024
TfiI	GAWTC	9 cut(s)	385, 544, 567, 787, 797, 922, 1006, 1207, 1612
Tru1I	TTAA	3 cut(s)	450, 830, 1413
Tru9I	TTAA	3 cut(s)	450, 830, 1413
TseFI	GTSAC	1 cut(s)	883
TseI	GCWGC	8 cut(s)	379, 782, 1196, 1213, 1328, 1448, 1451, 1594
Tsp45I	GTSAC	1 cut(s)	883
TspDTI	ATGAA	8 cut(s)	17, 263, 459, 1145, 1379, 1659, 1767, 1974
TspGWI	ACGGA	4 cut(s)	169, 408, 1564, 1910
VpaK11BI	GGWCC	1 cut(s)	598
XapI	RAATTY	4 cut(s)	650, 1000, 1291, 1979
XceI	RCATGY	2 cut(s)	219, 952
XcmI	CCANNNNNNNNNTGG	1 cut(s)	808
XhoI	CTCGAG	1 cut(s)	1141
XmnI	GAANNNNTTC	2 cut(s)	140, 174
XspI	CTAG	4 cut(s)	182, 1217, 1914, 2004
ZrmI	AGTACT	1 cut(s)	2026
Zsp2I	ATGCAT	2 cut(s)	1306, 1708

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.