MD06G1184600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011101
Physical Location & Seq
Reverse (-)
32269300 .. 32271401
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1184600.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGTGGCGATTTCGTTGTACAGAGGAAATCTCCACAGAGTTCCCGACGTACCACGTCGGTGGCTTATGCCAACCCCTAAAATCTCCCTGAAGGACTTCAAATCCCTTTTAACCCGCCGCTCTGTAGCTCTCTCCCGCCTCCGCTCCGGCCTCCCCGCCGCCGTCTCTTCTAACCCTAAGCCTGATTTTGTCCCGGGCCAGCTCAGCGAAGCTCCAAAGGCGGGAAATTTGTCTAACCCAACGATTAAATTCGAAGCGCCGGAGAATGTTGCTACTGATTGTGAAGCTGGGCCGTCCGGGGAGAAGAAGGATGGGAAGCAATCGGACGGTGCTGGTTTTGCGGTGAAATCCGGTGGTGGGTCCGATTCGCCGCCTGCTCTTAAGTCCGATTTGATCGAGAAAGGGTTGGATGTTGCAGATGTTGGTGCTAGTTCACTGCCTGTGAAGGTTGAGAAATTGGATGAAGTGGAAAACCCTAAATCTGAGAGATGCAACAAAGAGGAGGAGTTAAGTGATAAGCAAAAAAGGAAGAGAGAAGTTGAGGAGAAGCTGCAGGTTTTGAATCAGAAGAAACATAATCTTGTTCAAGTGTTGAAGCAGATCTTAAATGCAGAGGAAGAATTAAAGAGACGAAATTGTATGCAAGGAGTGGTGGTTCGCCCGTCTGTATCACCTCAAGTTGATGTAACAAATGACTCCGGTTCAATGACTAGGCATGCTGCTGCTAGGTTGGGCTCAGAGGCGAATCTTGAGATGGAAGGGGTTGAGGGCGATGAGCTTTCAAATCATAACTTTCATTCTCGCCATGTATTCCGGATGAACAGCACATCCCCATCTTCAGAGTCTCCTCTCAGAAGGCCGGCTCATATTCAGCACAACGTGGTTCCACACCCCTCTCGAGCAAGTTTAGGACCAACCGGCACTAGTCCATCACGCTTTGCTCCCATAGGGCAACAGGGGCATCCTGCAAATCTACCCACAGTATCTGTATCAGGAACCAATTACATGGCATCCTCTCCATCCCCTGCAGCTTCCGGCGGCACTTCTGCTTTCAAAGACGCTCGATTGCCAAGTCCATGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G_path_suppress

Protein Analysis

362

Amino Acids

38.87

Weight (kDa)

9.25

Isoelectric Point (pI)

66.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47850
fragaria_vesca FvH4_5g15910 FvH4_5g15910
malus_domestica MD06G1184600.v1.1 MD14G1190500.v1.1
prunus_persica Prupe.5G183400_v2.0.a1
pyrus_communis pycom06g16380 pycom14g15820
rosa_chinensis RchiOBHm_Chr7g0180141
rosa_laevigata RLG00000005289
rosa_multiflora Rmu_ssc0000119.1_g000036
rosa_roxburghii Rroxscaffold_3G00273090
rosa_rugosa Rorug06G0430800
rosa_samantha Rh6BG096200 Rh7AG031300 Rh7BG031300 Rh7BG031400 Rh7CG033200 Rh7DG031800
rosa_wichuraiana Rw7G002610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AccBSI CCGCTC 2 cut(s) 120, 144
AccIII TCCGGA 1 cut(s) 813
AcsI RAATTY 2 cut(s) 226, 248
AcuI CTGAAG 2 cut(s) 110, 822
AdeI CACNNNGTG 1 cut(s) 880
AfaI GTAC 2 cut(s) 20, 51
AfiI CCNNNNNNNGG 2 cut(s) 221, 357
AflII CTTAAG 1 cut(s) 380
AgsI TTSAA 7 cut(s) 100, 562, 587, 595, 705, 783, 1054
AhlI ACTAGT 1 cut(s) 923
AjiI CACGTC 1 cut(s) 56
AleI CACNNNNGTG 1 cut(s) 58
AloI GAACNNNNNNTCC 4 cut(s) 639, 671, 812, 844
AluBI AGCT 7 cut(s) 128, 202, 212, 287, 550, 778, 1031
AluI AGCT 7 cut(s) 128, 202, 212, 287, 550, 778, 1031
Alw26I GTCTC 3 cut(s) 169, 622, 849
AlwNI CAGNNNCTG 1 cut(s) 986
Ama87I CYCGRG 2 cut(s) 193, 897
Aor13HI TCCGGA 1 cut(s) 813
AoxI GGCC 4 cut(s) 148, 196, 290, 857
ApeKI GCWGC 4 cut(s) 550, 719, 722, 1028
ApoI RAATTY 2 cut(s) 226, 248
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 4 cut(s) 196, 290, 360, 911
AsuC2I CCSGG 3 cut(s) 194, 195, 298
AsuHPI GGTGA 2 cut(s) 355, 663
AsuII TTCGAA 1 cut(s) 252
AvaI CYCGRG 2 cut(s) 193, 897
AvaII GGWCC 2 cut(s) 360, 911
BanII GRGCYC 1 cut(s) 737
BbvI GCAGC 4 cut(s) 537, 706, 709, 1040
BccI CCATC 5 cut(s) 305, 748, 841, 937, 1027
BceAI ACGGC 2 cut(s) 146, 277
BcnI CCSGG 3 cut(s) 194, 195, 298
BcoDI GTCTC 3 cut(s) 169, 622, 849
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 4 cut(s) 429, 711, 726, 924
BfmI CTRYAG 3 cut(s) 123, 551, 1026
BfoI RGCGCY 1 cut(s) 260
BfrI CTTAAG 1 cut(s) 380
BfuAI ACCTGC 1 cut(s) 544
BglII AGATCT 1 cut(s) 600
BisI GCNGC 8 cut(s) 118, 159, 371, 551, 720, 723, 1029, 1039
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 8 cut(s) 119, 160, 372, 552, 721, 724, 1030, 1040
Bme1390I CCNGG 3 cut(s) 194, 195, 298
Bme18I GGWCC 2 cut(s) 360, 911
BmeT110I CYCGRG 2 cut(s) 193, 897
BmgBI CACGTC 1 cut(s) 56
BmgT120I GGNCC 4 cut(s) 196, 290, 360, 911
BmiI GGNNCC 3 cut(s) 361, 885, 997
BmrFI CCNGG 3 cut(s) 194, 195, 298
BmsI GCATC 3 cut(s) 479, 970, 1019
BplI GAGNNNNNCTC 2 cut(s) 15, 47
Bpu10I CCTNAGC 1 cut(s) 177
Bpu1102I GCTNAGC 1 cut(s) 203
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 660, 770
BpuMI CCSGG 3 cut(s) 194, 195, 298
BsaJI CCNNGG 3 cut(s) 193, 297, 1076
BsaWI WCCGGW 3 cut(s) 350, 698, 813
BsaXI ACNNNNNCTCC 2 cut(s) 639, 669
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 357
Bse118I RCCGGY 2 cut(s) 859, 917
BseAI TCCGGA 1 cut(s) 813
BseDI CCNNGG 3 cut(s) 193, 297, 1076
BseGI GGATG 8 cut(s) 316, 415, 466, 822, 827, 961, 1010, 1019
BseLI CCNNNNNNNGG 2 cut(s) 221, 357
BseMII CTCAG 4 cut(s) 217, 474, 750, 865
BseRI GAGGAG 4 cut(s) 515, 518, 557, 837
BseXI GCAGC 4 cut(s) 537, 706, 709, 1040
BseYI CCCAGC 1 cut(s) 287
BshFI GGCC 4 cut(s) 150, 198, 292, 859
BsiHKCI CYCGRG 2 cut(s) 193, 897
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 221, 357
BsmAI GTCTC 3 cut(s) 169, 622, 849
BsmBI CGTCTC 2 cut(s) 169, 622
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 4 cut(s) 150, 198, 292, 859
BsoBI CYCGRG 2 cut(s) 193, 897
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 737
Bsp13I TCCGGA 1 cut(s) 813
Bsp1407I TGTACA 1 cut(s) 18
Bsp143I GATC 2 cut(s) 393, 600
Bsp1720I GCTNAGC 1 cut(s) 203
Bsp19I CCATGG 1 cut(s) 1076
BspANI GGCC 4 cut(s) 150, 198, 292, 859
BspCNI CTCAG 4 cut(s) 216, 475, 749, 864
BspEI TCCGGA 1 cut(s) 813
BspLI GGNNCC 3 cut(s) 361, 885, 997
BspMAI CTGCAG 2 cut(s) 555, 1030
BspMI ACCTGC 1 cut(s) 544
BspT104I TTCGAA 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 380
BsrBI CCGCTC 2 cut(s) 120, 144
BsrFI RCCGGY 2 cut(s) 859, 917
BsrGI TGTACA 1 cut(s) 18
BssAI RCCGGY 2 cut(s) 859, 917
BssECI CCNNGG 3 cut(s) 193, 297, 1076
BssMI GATC 2 cut(s) 393, 600
BssT1I CCWWGG 1 cut(s) 1076
Bst4CI ACNGT 2 cut(s) 329, 982
Bst6I CTCTTC 2 cut(s) 172, 524
BstAFI CTTAAG 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 18
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 4 cut(s) 200, 375, 717, 861
BstDEI CTNAG 5 cut(s) 177, 203, 483, 736, 851
BstDSI CCRYGG 1 cut(s) 1076
BstF5I GGATG 8 cut(s) 316, 415, 466, 822, 827, 961, 1010, 1019
BstH2I RGCGCY 1 cut(s) 260
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 2 cut(s) 396, 603
BstMAI GTCTC 3 cut(s) 169, 622, 849
BstMBI GATC 2 cut(s) 393, 600
BstMWI GCNNNNNNNGC 4 cut(s) 204, 218, 338, 958
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 192, 193, 296
BstSFI CTRYAG 3 cut(s) 123, 551, 1026
BstV1I GCAGC 4 cut(s) 537, 706, 709, 1040
BstX2I RGATCY 1 cut(s) 600
BstXI CCANNNNNNTGG 3 cut(s) 60, 1006, 1077
BstYI RGATCY 1 cut(s) 600
BsuRI GGCC 4 cut(s) 150, 198, 292, 859
BtgI CCRYGG 1 cut(s) 1076
BtgZI GCGATG 1 cut(s) 786
BtrI CACGTC 1 cut(s) 56
BtsCI GGATG 8 cut(s) 316, 415, 466, 822, 827, 961, 1010, 1019
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 1 cut(s) 434
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 200, 375, 717, 861
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 1 cut(s) 259
Cfr10I RCCGGY 2 cut(s) 859, 917
Cfr13I GGNCC 4 cut(s) 196, 290, 360, 911
Cfr9I CCCGGG 1 cut(s) 193
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 2 cut(s) 19, 50
CviAII CATG 4 cut(s) 716, 806, 1006, 1077
CviQI GTAC 2 cut(s) 19, 50
DdeI CTNAG 5 cut(s) 177, 203, 483, 736, 851
DpnI GATC 2 cut(s) 395, 602
DpnII GATC 2 cut(s) 393, 600
DraIII CACNNNGTG 1 cut(s) 880
Eam1104I CTCTTC 2 cut(s) 172, 524
EarI CTCTTC 2 cut(s) 172, 524
Eco130I CCWWGG 1 cut(s) 1076
Eco24I GRGCYC 1 cut(s) 737
Eco47I GGWCC 2 cut(s) 360, 911
Eco57I CTGAAG 2 cut(s) 110, 822
Eco88I CYCGRG 2 cut(s) 193, 897
EcoT14I CCWWGG 1 cut(s) 1076
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 1076
Esp3I CGTCTC 2 cut(s) 169, 622
FaeI CATG 4 cut(s) 719, 809, 1009, 1080
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FaqI GGGAC 1 cut(s) 176
FatI CATG 4 cut(s) 715, 805, 1005, 1076
FauI CCCGC 4 cut(s) 122, 143, 163, 214
Fnu4HI GCNGC 8 cut(s) 118, 159, 371, 551, 720, 723, 1029, 1039
FokI GGATG 8 cut(s) 323, 422, 473, 814, 829, 948, 997, 1006
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 8 cut(s) 118, 159, 371, 551, 720, 723, 1029, 1039
FspBI CTAG 4 cut(s) 429, 711, 726, 924
GlaI GCGC 1 cut(s) 258
GluI GCNGC 8 cut(s) 118, 159, 371, 551, 720, 723, 1029, 1039
GsaI CCCAGC 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 260
HaeIII GGCC 4 cut(s) 150, 198, 292, 859
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 1 cut(s) 259
Hin1II CATG 4 cut(s) 719, 809, 1009, 1080
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HinfI GANTC 5 cut(s) 365, 562, 695, 745, 842
HphI GGTGA 2 cut(s) 355, 663
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 8 cut(s) 325, 364, 388, 484, 567, 739, 841, 854
Hpy188III TCNNGA 6 cut(s) 44, 397, 749, 814, 897, 993
Hpy8I GTNNAC 1 cut(s) 434
Hpy99I CGWCG 2 cut(s) 50, 60
HpyAV CCTTC 5 cut(s) 85, 301, 439, 752, 849
HpyCH4III ACNGT 2 cut(s) 329, 982
HpyCH4IV ACGT 3 cut(s) 48, 55, 879
HpyCH4V TGCA 7 cut(s) 416, 492, 553, 611, 643, 968, 1028
HpyF10VI GCNNNNNNNGC 4 cut(s) 204, 218, 338, 958
HpyF3I CTNAG 5 cut(s) 177, 203, 483, 736, 851
HpySE526I ACGT 3 cut(s) 48, 55, 879
Hsp92II CATG 4 cut(s) 719, 809, 1009, 1080
HspAI GCGC 1 cut(s) 257
Kpn2I TCCGGA 1 cut(s) 813
KroI GCCGGC 1 cut(s) 859
KroNI GCCGGC 1 cut(s) 861
Kzo9I GATC 2 cut(s) 393, 600
LmnI GCTCC 3 cut(s) 149, 217, 946
Lsp1109I GCAGC 4 cut(s) 537, 706, 709, 1040
LweI GCATC 3 cut(s) 479, 970, 1019
MaeI CTAG 4 cut(s) 429, 711, 726, 924
MaeII ACGT 3 cut(s) 48, 55, 879
MaeIII GTNAC 1 cut(s) 685
MalI GATC 2 cut(s) 395, 602
MbiI CCGCTC 2 cut(s) 120, 144
MboI GATC 2 cut(s) 393, 600
MboII GAAGA 6 cut(s) 159, 316, 541, 580, 629, 828
MflI RGATCY 1 cut(s) 600
MhlI GDGCHC 1 cut(s) 737
MluCI AATT 7 cut(s) 226, 248, 455, 620, 634, 1000, 1081
MlyI GAGTC 2 cut(s) 689, 851
MmeI TCCRAC 1 cut(s) 387
MroI TCCGGA 1 cut(s) 813
MroNI GCCGGC 1 cut(s) 859
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 6 cut(s) 110, 246, 381, 509, 605, 623
MslI CAYNNNNRTG 1 cut(s) 58
MspCI CTTAAG 1 cut(s) 380
MspR9I CCNGG 3 cut(s) 194, 195, 298
MwoI GCNNNNNNNGC 4 cut(s) 204, 218, 338, 958
NaeI GCCGGC 1 cut(s) 861
NciI CCSGG 3 cut(s) 194, 195, 298
NcoI CCATGG 1 cut(s) 1076
NdeII GATC 2 cut(s) 393, 600
NgoMIV GCCGGC 1 cut(s) 859
NlaIII CATG 4 cut(s) 719, 809, 1009, 1080
NlaIV GGNNCC 3 cut(s) 361, 885, 997
NspI RCATGY 1 cut(s) 719
NspV TTCGAA 1 cut(s) 252
OliI CACNNNNGTG 1 cut(s) 58
PaeI GCATGC 1 cut(s) 719
PaeR7I CTCGAG 1 cut(s) 897
PdiI GCCGGC 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 3 cut(s) 365, 562, 745
PkrI GCNGC 8 cut(s) 119, 160, 372, 552, 721, 724, 1030, 1040
PleI GAGTC 2 cut(s) 689, 850
PpsI GAGTC 2 cut(s) 689, 850
PspFI CCCAGC 1 cut(s) 287
PspN4I GGNNCC 3 cut(s) 361, 885, 997
PspPI GGNCC 4 cut(s) 196, 290, 360, 911
PstI CTGCAG 2 cut(s) 555, 1030
PstNI CAGNNNCTG 1 cut(s) 986
PsuI RGATCY 1 cut(s) 600
RsaI GTAC 2 cut(s) 20, 51
RsaNI GTAC 2 cut(s) 19, 50
RseI CAYNNNNRTG 1 cut(s) 58
SaqAI TTAA 6 cut(s) 110, 246, 381, 509, 605, 623
SatI GCNGC 8 cut(s) 118, 159, 371, 551, 720, 723, 1029, 1039
Sau3AI GATC 2 cut(s) 393, 600
Sau96I GGNCC 4 cut(s) 196, 290, 360, 911
SchI GAGTC 2 cut(s) 689, 851
ScrFI CCNGG 3 cut(s) 194, 195, 298
SduI GDGCHC 1 cut(s) 737
SfaNI GCATC 3 cut(s) 479, 970, 1019
SfcI CTRYAG 3 cut(s) 123, 551, 1026
Sfr274I CTCGAG 1 cut(s) 897
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 2 cut(s) 360, 911
SlaI CTCGAG 1 cut(s) 897
SmaI CCCGGG 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 58
SmlI CTYRAG 4 cut(s) 380, 675, 749, 897
SmoI CTYRAG 4 cut(s) 380, 675, 749, 897
SpeI ACTAGT 1 cut(s) 923
SphI GCATGC 1 cut(s) 719
Sse9I AATT 7 cut(s) 226, 248, 455, 620, 634, 1000, 1081
SspMI CTAG 4 cut(s) 429, 711, 726, 924
StyD4I CCNGG 3 cut(s) 192, 193, 296
StyI CCWWGG 1 cut(s) 1076
TaaI ACNGT 2 cut(s) 329, 982
TaiI ACGT 3 cut(s) 51, 58, 882
TaqI TCGA 4 cut(s) 252, 396, 898, 1063
TasI AATT 7 cut(s) 226, 248, 455, 620, 634, 1000, 1081
TatI WGTACW 1 cut(s) 18
TauI GCSGC 4 cut(s) 120, 161, 373, 1041
TfiI GAWTC 3 cut(s) 365, 562, 745
Tru1I TTAA 6 cut(s) 110, 246, 381, 509, 605, 623
Tru9I TTAA 6 cut(s) 110, 246, 381, 509, 605, 623
TscAI CASTG 1 cut(s) 441
TseI GCWGC 4 cut(s) 550, 719, 722, 1028
TspDTI ATGAA 3 cut(s) 477, 785, 833
TspMI CCCGGG 1 cut(s) 193
TspRI CASTG 1 cut(s) 441
Vha464I CTTAAG 1 cut(s) 380
VpaK11BI GGWCC 2 cut(s) 360, 911
XapI RAATTY 2 cut(s) 226, 248
XceI RCATGY 1 cut(s) 719
XhoI CTCGAG 1 cut(s) 897
XmaI CCCGGG 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 4 cut(s) 429, 711, 726, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.