Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001264

Physical Location & Seq

Genomic Coordinates Reverse (-)

35898325 .. 35902567

Gene Structure 4243 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1229100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGAGTACTCCTTTGTGCATGCTTCTAGGTTTTCTTGTGATACAGTGCTTCGCGCATGCGGCGGAGCACAATGGAGCTTCAGTTCTTGAGCCGGAGAGTGTGAAATTGGACACAGGTGGGCTGAGCAGAGCCAGCTTCCGCAAGGATTTTGTGTTCGGAACGGCGACGTCCGCTTACCAAGTTGAAGGCATGGCCAACAAGGAGGGTCGAGGGCCCAGCATTTGGGATGCATTTGTTAAAATTCCCGGAATTATTGCAAATAATGGTACAGCAGATGTTGCTGTGGACCAGTATCACCGATACAAAGACGATATTGATCTCATGGCAAGCCTAAATTTCGATGCCTATCGATTCTCAATATCATGGTCCAGAATTTTTCCAAATGGCACTGGGAATGTAAATTGGAAGGGAGTTGCATACTACAATCGGTTAATTAACTACTTGCTCAAAAGAGGCATTACTCCATATGCAAATTTATATCATTATGATCTTCCCCTAGCTCTTGAGAAGAAGTACTTGGGATTGCTAAGTGACAAAGTGGTGAAAGATTTTGCTGATTATGCAGACTTTTGTTTCAAGACGTTTGGAGACAGAGTGAAGAACTGGATGACCTTCAATGAGCCTAGAGTGATTGCTGCTCTTGGATATGATAACGGATTCCATGCTCCTGGACGATGCTCCAAAGCATTTGGAAATTGCACGGCTGGAAATTCAGCAACCGAGCCTTATATTGCTGCTCATCATTTGATTTTGTCACACGGAGCTGCCGTTCAGAGATACCGCGAAAAGTATCAGAAAATACAAAAAGGAAGGATTGGTATTCTCTTGGATTTTGTTTGGTATGAGCCTCTTACAAGATCAAAGGCTGACAACCTTGCAGCTCAAAGGGCCAGAGACTTTCATGTTGGATGGTTCATCCACCCGATTGTTTATGGAGAGTATCCGAAAACAGTCCAAGAAATTGTTGGTGACAGGCTGCCTAAGTTCACCAAAGAGGAGGTTAAGATGGTGAAGGGTTCAATGGATTATGTTGGAATCAACCAATATACTACTTACTACATGTATCACCCAAAACCATCAAAATCAAATGTCTTGGGGTACCAGCAGGATTGGAATGCAGGATTTGCTTATGCAAAGAATGGAGTGCCTATTGGTCCTAGGGCATATTCTTATTGGCTGTACCAAGTGCCATGGGGTTTGTACAAAGCTGTTATGTACATAAAAGAGCATTATGGAAATCCCCCAGTGATTTTATCTGAAAATGGAATGGACGACCCTGGCAATGTGACACTTGCGGAGGGATTGCGCGACACGACAAGGGCCAACTTCTACAGGAGTTACATAACTCAACTGAAGAAGGCAGCAGATGACGGAGCCAATGTGATGGGCTACTTCGCTTGGTCGTTGCTCGATAACTTTGAATGGAGGCTGGGATACACTTCCAGATTTGGCATTGTCTACGTAGATTTCAAAACCCTCAAGAGATATCCAAAGATGTCCGCAAACTGGTTCAAGAAGCTACTTACCAGAAACAAGCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0008150 GO:0008152 GO:0008422 GO:0009505 GO:0015923 GO:0015926 GO:0016787 GO:0016798 GO:0022626 GO:0030312 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0047668 GO:0071704 GO:0071944 GO:0080079 GO:0080081 GO:0080082 GO:0080083 GO:1901135 GO:1901657 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

514

Amino Acids

58.47

Weight (kDa)

9.23

Isoelectric Point (pI)

20.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	44 - 512	6.3e-155	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001264)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18070 AT3G18070 AT3G18070 AT3G18070 AT3G18070 AT3G18070 AT3G18070 AT3G18080
fragaria_vesca	FvH4_5g17090 FvH4_5g17090 FvH4_5g17090 FvH4_5g17090 FvH4_5g17090 FvH4_5g17090 FvH4_5g17090 FvH4_5g17090 FvH4_5g17090
malus_domestica	MD06G1229100.v1.1
prunus_persica	Prupe.5G230400_v2.0.a1
pyrus_communis	pycom06g20030
rosa_chinensis	RchiOBHm_Chr7g0178721 RchiOBHm_Chr7g0178731 RchiOBHm_Chr7g0178741 RchiOBHm_Chr7g0178751
rosa_laevigata	RLG00000005418 RLG00000005419
rosa_multiflora	Rmu_co8186916.1_g000001 Rmu_co8266963.1_g000001 Rmu_sc0002320.1_g000021 Rmu_sc0002320.1_g000022 Rmu_sc0002320.1_g000023 Rmu_sc0002320.1_g000027 Rmu_sc0025325.1_g000010 Rmu_sc0025325.1_g000011
rosa_roxburghii	Rroxscaffold_3G00274640
rosa_rugosa	Rorug06G0418900 Rorug06G0419000 Rorug06G0419100
rosa_samantha	Rh2AG182000 Rh2AG203200 Rh2AG531500 Rh5CG480100 Rh7AG018300 Rh7AG018400 Rh7AG018500 Rh7AG018600 Rh7BG017900 Rh7BG018000 Rh7CG018600 Rh7CG018800 Rh7DG018400 Rh7DG018500
rosa_wichuraiana	Rw7G001480 Rw7G001490 Rw7G001500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	170
Acc65I	GGTACC	1 cut(s)	1098
AccB1I	GGYRCC	1 cut(s)	1098
AccB7I	CCANNNNNTGG	1 cut(s)	222
AccI	GTMKAC	1 cut(s)	1458
AccII	CGCG	3 cut(s)	53, 783, 1308
AciI	CCGC	7 cut(s)	59, 62, 139, 171, 781, 1295, 1500
AcoI	YGGCCR	1 cut(s)	192
AcsI	RAATTY	5 cut(s)	240, 334, 372, 472, 709
AcuI	CTGAAG	2 cut(s)	63, 1373
AcyI	GRCGYC	1 cut(s)	167
AfaI	GTAC	7 cut(s)	7, 268, 515, 1100, 1181, 1202, 1217
AfiI	CCNNNNNNNGG	2 cut(s)	222, 1506
AflIII	ACRYGT	1 cut(s)	1059
AgsI	TTSAA	7 cut(s)	185, 577, 616, 1020, 1421, 1471, 1513
AjnI	CCWGG	2 cut(s)	667, 1276
AjuI	GAANNNNNNNTTGG	2 cut(s)	500, 532
AloI	GAACNNNNNNTCC	6 cut(s)	66, 98, 137, 169, 753, 785
AluBI	AGCT	7 cut(s)	77, 135, 500, 764, 881, 1208, 1519
AluI	AGCT	7 cut(s)	77, 135, 500, 764, 881, 1208, 1519
Alw21I	GWGCWC	1 cut(s)	69
Alw26I	GTCTC	2 cut(s)	582, 888
AoxI	GGCC	4 cut(s)	192, 212, 888, 1320
ApaI	GGGCCC	1 cut(s)	216
ApeKI	GCWGC	6 cut(s)	635, 734, 764, 878, 976, 1361
ApoI	RAATTY	5 cut(s)	240, 334, 372, 472, 709
Asp718I	GGTACC	1 cut(s)	1098
AspA2I	CCTAGG	1 cut(s)	1157
AspLEI	GCGC	2 cut(s)	55, 1308
AspS9I	GGNCC	7 cut(s)	212, 213, 286, 366, 888, 1154, 1320
AsuC2I	CCSGG	1 cut(s)	246
AsuHPI	GGTGA	6 cut(s)	287, 553, 979, 980, 1021, 1058
AvaII	GGWCC	3 cut(s)	286, 366, 1154
AvrII	CCTAGG	1 cut(s)	1157
BaeGI	GKGCMC	1 cut(s)	216
BalI	TGGCCA	1 cut(s)	194
BanI	GGYRCC	1 cut(s)	1098
BanII	GRGCYC	1 cut(s)	216
Bbv12I	GWGCWC	1 cut(s)	69
BbvI	GCAGC	6 cut(s)	622, 721, 751, 890, 963, 1373
BccI	CCATC	4 cut(s)	903, 1000, 1084, 1378
BceAI	ACGGC	3 cut(s)	177, 717, 752
BciT130I	CCWGG	2 cut(s)	669, 1278
BciVI	GTATCC	2 cut(s)	951, 1427
BcnI	CCSGG	1 cut(s)	246
BcoDI	GTCTC	2 cut(s)	582, 888
BfaI	CTAG	4 cut(s)	26, 497, 624, 1158
BfmI	CTRYAG	1 cut(s)	1330
BfuI	GTATCC	2 cut(s)	951, 1427
BisI	GCNGC	7 cut(s)	60, 636, 735, 765, 879, 977, 1362
BlnI	CCTAGG	1 cut(s)	1157
BlpI	GCTNAGC	1 cut(s)	122
BlsI	GCNGC	7 cut(s)	61, 637, 736, 766, 880, 978, 1363
BmcAI	AGTACT	2 cut(s)	7, 515
Bme1390I	CCNGG	3 cut(s)	246, 669, 1278
Bme18I	GGWCC	3 cut(s)	286, 366, 1154
BmgT120I	GGNCC	7 cut(s)	212, 213, 286, 366, 888, 1154, 1320
BmiI	GGNNCC	3 cut(s)	214, 1100, 1375
BmrFI	CCNGG	3 cut(s)	246, 669, 1278
BmrI	ACTGGG	2 cut(s)	399, 1238
BmsI	GCATC	3 cut(s)	217, 331, 665
BmuI	ACTGGG	2 cut(s)	399, 1238
Bpu1102I	GCTNAGC	1 cut(s)	122
BpuEI	CTTGAG	3 cut(s)	107, 524, 1463
BpuMI	CCSGG	1 cut(s)	246
Bsa29I	ATCGAT	1 cut(s)	349
BsaAI	YACGTR	1 cut(s)	1462
BsaBI	GATNNNNATC	2 cut(s)	315, 345
BsaHI	GRCGYC	1 cut(s)	167
BsaJI	CCNNGG	3 cut(s)	1157, 1190, 1276
BsaXI	ACNNNNNCTCC	8 cut(s)	66, 96, 579, 609, 753, 783, 1365, 1395
Bsc4I	CCNNNNNNNGG	2 cut(s)	222, 1506
Bse1I	ACTGG	5 cut(s)	289, 394, 608, 1244, 1511
Bse3DI	GCAATG	1 cut(s)	1288
Bse8I	GATNNNNATC	2 cut(s)	315, 345
BseBI	CCWGG	2 cut(s)	669, 1278
BseCI	ATCGAT	1 cut(s)	349
BseDI	CCNNGG	3 cut(s)	1157, 1190, 1276
BseGI	GGATG	4 cut(s)	232, 612, 914, 915
BseJI	GATNNNNATC	2 cut(s)	315, 345
BseLI	CCNNNNNNNGG	2 cut(s)	222, 1506
BseMI	GCAATG	1 cut(s)	1288
BseMII	CTCAG	1 cut(s)	113
BseNI	ACTGG	5 cut(s)	289, 394, 608, 1244, 1511
BseRI	GAGGAG	1 cut(s)	1010
BseSI	GKGCMC	1 cut(s)	216
BseXI	GCAGC	6 cut(s)	622, 721, 751, 890, 963, 1373
BseYI	CCCAGC	2 cut(s)	215, 1429
Bsh1236I	CGCG	3 cut(s)	53, 783, 1308
BshFI	GGCC	4 cut(s)	194, 214, 890, 1322
BshNI	GGYRCC	1 cut(s)	1098
BshVI	ATCGAT	1 cut(s)	349
BsiHKAI	GWGCWC	1 cut(s)	69
BsiSI	CCGG	2 cut(s)	92, 246
BslI	CCNNNNNNNGG	2 cut(s)	222, 1506
BsmAI	GTCTC	2 cut(s)	582, 888
BsmI	GAATGC	1 cut(s)	1120
BsnI	GGCC	4 cut(s)	194, 214, 890, 1322
Bsp120I	GGGCCC	1 cut(s)	212
Bsp1286I	GDGCHC	2 cut(s)	69, 216
Bsp1407I	TGTACA	2 cut(s)	1200, 1215
Bsp143I	GATC	3 cut(s)	316, 487, 857
Bsp1720I	GCTNAGC	1 cut(s)	122
Bsp19I	CCATGG	1 cut(s)	1190
BspACI	CCGC	7 cut(s)	59, 62, 139, 171, 781, 1295, 1500
BspANI	GGCC	4 cut(s)	194, 214, 890, 1322
BspCNI	CTCAG	1 cut(s)	114
BspDI	ATCGAT	1 cut(s)	349
BspFNI	CGCG	3 cut(s)	53, 783, 1308
BspLI	GGNNCC	3 cut(s)	214, 1100, 1375
BspT107I	GGYRCC	1 cut(s)	1098
BsrDI	GCAATG	1 cut(s)	1288
BsrGI	TGTACA	2 cut(s)	1200, 1215
BsrI	ACTGG	5 cut(s)	289, 394, 608, 1244, 1511
BssECI	CCNNGG	3 cut(s)	1157, 1190, 1276
BssMI	GATC	3 cut(s)	316, 487, 857
BssNI	GRCGYC	1 cut(s)	167
BssT1I	CCWWGG	2 cut(s)	1157, 1190
Bst2UI	CCWGG	2 cut(s)	669, 1278
Bst4CI	ACNGT	2 cut(s)	45, 952
BstACI	GRCGYC	1 cut(s)	167
BstAPI	GCANNNNNTGC	2 cut(s)	278, 1124
BstAUI	TGTACA	2 cut(s)	1200, 1215
BstBAI	YACGTR	1 cut(s)	1462
BstC8I	GCNNGC	4 cut(s)	20, 57, 133, 328
BstDEI	CTNAG	3 cut(s)	122, 527, 981
BstDSI	CCRYGG	1 cut(s)	1190
BstF5I	GGATG	4 cut(s)	232, 612, 914, 915
BstFNI	CGCG	3 cut(s)	53, 783, 1308
BstHHI	GCGC	2 cut(s)	55, 1308
BstKTI	GATC	3 cut(s)	319, 490, 860
BstMAI	GTCTC	2 cut(s)	582, 888
BstMBI	GATC	3 cut(s)	316, 487, 857
BstMWI	GCNNNNNNNGC	7 cut(s)	59, 132, 170, 278, 560, 887, 1124
BstNI	CCWGG	2 cut(s)	669, 1278
BstNSI	RCATGY	3 cut(s)	22, 59, 1063
BstSCI	CCNGG	3 cut(s)	244, 667, 1276
BstSFI	CTRYAG	1 cut(s)	1330
BstSLI	GKGCMC	1 cut(s)	216
BstSNI	TACGTA	1 cut(s)	1462
BstUI	CGCG	3 cut(s)	53, 783, 1308
BstV1I	GCAGC	6 cut(s)	622, 721, 751, 890, 963, 1373
BstXI	CCANNNNNNTGG	2 cut(s)	668, 1384
Bsu15I	ATCGAT	1 cut(s)	349
BsuI	GTATCC	2 cut(s)	951, 1427
BsuRI	GGCC	4 cut(s)	194, 214, 890, 1322
BsuTUI	ATCGAT	1 cut(s)	349
BtgI	CCRYGG	1 cut(s)	1190
BtsCI	GGATG	4 cut(s)	232, 612, 914, 915
BtsIMutI	CAGTG	3 cut(s)	50, 387, 1251
Cac8I	GCNNGC	4 cut(s)	20, 57, 133, 328
CfoI	GCGC	2 cut(s)	55, 1308
Cfr13I	GGNCC	7 cut(s)	212, 213, 286, 366, 888, 1154, 1320
ClaI	ATCGAT	1 cut(s)	349
Csp6I	GTAC	7 cut(s)	6, 267, 514, 1099, 1180, 1201, 1216
CviAII	CATG	9 cut(s)	19, 56, 190, 322, 363, 662, 902, 1060, 1191
CviQI	GTAC	7 cut(s)	6, 267, 514, 1099, 1180, 1201, 1216
DdeI	CTNAG	3 cut(s)	122, 527, 981
DpnI	GATC	3 cut(s)	318, 489, 859
DpnII	GATC	3 cut(s)	316, 487, 857
EaeI	YGGCCR	1 cut(s)	192
EciI	GGCGGA	1 cut(s)	77
Eco105I	TACGTA	1 cut(s)	1462
Eco130I	CCWWGG	2 cut(s)	1157, 1190
Eco24I	GRGCYC	1 cut(s)	216
Eco32I	GATATC	1 cut(s)	1487
Eco47I	GGWCC	3 cut(s)	286, 366, 1154
Eco57I	CTGAAG	2 cut(s)	63, 1373
EcoO109I	RGGNCCY	1 cut(s)	212
EcoRII	CCWGG	2 cut(s)	667, 1276
EcoRV	GATATC	1 cut(s)	1487
EcoT14I	CCWWGG	2 cut(s)	1157, 1190
EcoT22I	ATGCAT	1 cut(s)	232
EcoT38I	GRGCYC	1 cut(s)	216
ErhI	CCWWGG	2 cut(s)	1157, 1190
FaeI	CATG	9 cut(s)	22, 59, 193, 325, 366, 665, 905, 1063, 1194
FalI	AAGNNNNNCTT	2 cut(s)	500, 532
FatI	CATG	9 cut(s)	18, 55, 189, 321, 362, 661, 901, 1059, 1190
FauNDI	CATATG	1 cut(s)	466
FblI	GTMKAC	1 cut(s)	1458
Fnu4HI	GCNGC	7 cut(s)	60, 636, 735, 765, 879, 977, 1362
FokI	GGATG	4 cut(s)	239, 619, 902, 921
FriOI	GRGCYC	1 cut(s)	216
Fsp4HI	GCNGC	7 cut(s)	60, 636, 735, 765, 879, 977, 1362
FspBI	CTAG	4 cut(s)	26, 497, 624, 1158
GlaI	GCGC	2 cut(s)	54, 1307
GluI	GCNGC	7 cut(s)	60, 636, 735, 765, 879, 977, 1362
GsaI	CCCAGC	2 cut(s)	219, 1433
HaeIII	GGCC	4 cut(s)	194, 214, 890, 1322
HapII	CCGG	2 cut(s)	92, 246
HhaI	GCGC	2 cut(s)	55, 1308
Hin1I	GRCGYC	1 cut(s)	167
Hin1II	CATG	9 cut(s)	22, 59, 193, 325, 366, 665, 905, 1063, 1194
Hin6I	GCGC	2 cut(s)	53, 1306
HinP1I	GCGC	2 cut(s)	53, 1306
HinfI	GANTC	3 cut(s)	351, 657, 1035
HpaII	CCGG	2 cut(s)	92, 246
HphI	GGTGA	6 cut(s)	287, 553, 979, 980, 1021, 1058
Hpy166II	GTNNAC	3 cut(s)	286, 987, 1459
Hpy188I	TCNGA	5 cut(s)	158, 774, 795, 945, 1258
Hpy188III	TCNNGA	7 cut(s)	86, 369, 503, 577, 1443, 1480, 1513
Hpy8I	GTNNAC	3 cut(s)	286, 987, 1459
Hpy99I	CGWCG	1 cut(s)	169
HpyAV	CCTTC	6 cut(s)	179, 400, 622, 804, 1006, 1351
HpyCH4III	ACNGT	2 cut(s)	45, 952
HpyCH4IV	ACGT	3 cut(s)	167, 581, 1461
HpyF10VI	GCNNNNNNNGC	7 cut(s)	59, 132, 170, 278, 560, 887, 1124
HpyF3I	CTNAG	3 cut(s)	122, 527, 981
HpySE526I	ACGT	3 cut(s)	167, 581, 1461
Hsp92I	GRCGYC	1 cut(s)	167
Hsp92II	CATG	9 cut(s)	22, 59, 193, 325, 366, 665, 905, 1063, 1194
HspAI	GCGC	2 cut(s)	53, 1306
KpnI	GGTACC	1 cut(s)	1102
Kzo9I	GATC	3 cut(s)	316, 487, 857
LmnI	GCTCC	6 cut(s)	64, 74, 670, 683, 761, 1373
Lsp1109I	GCAGC	6 cut(s)	622, 721, 751, 890, 963, 1373
LweI	GCATC	3 cut(s)	217, 331, 665
MaeI	CTAG	4 cut(s)	26, 497, 624, 1158
MaeII	ACGT	3 cut(s)	167, 581, 1461
MaeIII	GTNAC	5 cut(s)	530, 753, 968, 1285, 1337
MalI	GATC	3 cut(s)	318, 489, 859
MboI	GATC	3 cut(s)	316, 487, 857
MboII	GAAGA	4 cut(s)	482, 520, 610, 1366
MhlI	GDGCHC	2 cut(s)	69, 216
MlsI	TGGCCA	1 cut(s)	194
MluNI	TGGCCA	1 cut(s)	194
MmeI	TCCRAC	2 cut(s)	886, 1012
MnlI	CCTC	9 cut(s)	196, 203, 446, 858, 988, 991, 1291, 1419, 1487
Mox20I	TGGCCA	1 cut(s)	194
Mph1103I	ATGCAT	1 cut(s)	232
MscI	TGGCCA	1 cut(s)	194
MseI	TTAA	4 cut(s)	237, 431, 435, 1002
Msp20I	TGGCCA	1 cut(s)	194
MspI	CCGG	2 cut(s)	92, 246
MspR9I	CCNGG	3 cut(s)	246, 669, 1278
Mva1269I	GAATGC	1 cut(s)	1120
MvaI	CCWGG	2 cut(s)	669, 1278
MvnI	CGCG	3 cut(s)	53, 783, 1308
MwoI	GCNNNNNNNGC	7 cut(s)	59, 132, 170, 278, 560, 887, 1124
NciI	CCSGG	1 cut(s)	246
NcoI	CCATGG	1 cut(s)	1190
NdeI	CATATG	1 cut(s)	466
NdeII	GATC	3 cut(s)	316, 487, 857
NlaIII	CATG	9 cut(s)	22, 59, 193, 325, 366, 665, 905, 1063, 1194
NlaIV	GGNNCC	3 cut(s)	214, 1100, 1375
NmuCI	GTSAC	4 cut(s)	530, 753, 968, 1285
NsiI	ATGCAT	1 cut(s)	232
NspI	RCATGY	3 cut(s)	22, 59, 1063
PacI	TTAATTAA	1 cut(s)	435
PaeI	GCATGC	2 cut(s)	22, 59
PciI	ACATGT	1 cut(s)	1059
PcsI	WCGNNNNNNNCGW	1 cut(s)	765
PctI	GAATGC	1 cut(s)	1120
PfeI	GAWTC	3 cut(s)	351, 657, 1035
PflMI	CCANNNNNTGG	1 cut(s)	222
PfoI	TCCNGGA	2 cut(s)	244, 667
PkrI	GCNGC	7 cut(s)	61, 637, 736, 766, 880, 978, 1363
Ppu21I	YACGTR	1 cut(s)	1462
PscI	ACATGT	1 cut(s)	1059
Psp6I	CCWGG	2 cut(s)	667, 1276
PspFI	CCCAGC	2 cut(s)	215, 1429
PspGI	CCWGG	2 cut(s)	667, 1276
PspN4I	GGNNCC	3 cut(s)	214, 1100, 1375
PspOMI	GGGCCC	1 cut(s)	212
PspPI	GGNCC	7 cut(s)	212, 213, 286, 366, 888, 1154, 1320
RsaI	GTAC	7 cut(s)	7, 268, 515, 1100, 1181, 1202, 1217
RsaNI	GTAC	7 cut(s)	6, 267, 514, 1099, 1180, 1201, 1216
SaqAI	TTAA	4 cut(s)	237, 431, 435, 1002
SatI	GCNGC	7 cut(s)	60, 636, 735, 765, 879, 977, 1362
Sau3AI	GATC	3 cut(s)	316, 487, 857
Sau96I	GGNCC	7 cut(s)	212, 213, 286, 366, 888, 1154, 1320
ScaI	AGTACT	2 cut(s)	7, 515
ScrFI	CCNGG	3 cut(s)	246, 669, 1278
SduI	GDGCHC	2 cut(s)	69, 216
SfaNI	GCATC	3 cut(s)	217, 331, 665
SfcI	CTRYAG	1 cut(s)	1330
SinI	GGWCC	3 cut(s)	286, 366, 1154
SmlI	CTYRAG	3 cut(s)	86, 503, 1478
SmoI	CTYRAG	3 cut(s)	86, 503, 1478
SnaBI	TACGTA	1 cut(s)	1462
SphI	GCATGC	2 cut(s)	22, 59
SsiI	CCGC	7 cut(s)	59, 62, 139, 171, 781, 1295, 1500
SspMI	CTAG	4 cut(s)	26, 497, 624, 1158
StyD4I	CCNGG	3 cut(s)	244, 667, 1276
StyI	CCWWGG	2 cut(s)	1157, 1190
TaaI	ACNGT	2 cut(s)	45, 952
TaiI	ACGT	3 cut(s)	170, 584, 1464
TaqI	TCGA	4 cut(s)	208, 339, 349, 1410
TatI	WGTACW	4 cut(s)	5, 513, 1200, 1215
TauI	GCSGC	1 cut(s)	62
TfiI	GAWTC	3 cut(s)	351, 657, 1035
Tru1I	TTAA	4 cut(s)	237, 431, 435, 1002
Tru9I	TTAA	4 cut(s)	237, 431, 435, 1002
TscAI	CASTG	3 cut(s)	50, 394, 1251
TseFI	GTSAC	4 cut(s)	530, 753, 968, 1285
TseI	GCWGC	6 cut(s)	635, 734, 764, 878, 976, 1361
Tsp45I	GTSAC	4 cut(s)	530, 753, 968, 1285
TspDTI	ATGAA	2 cut(s)	890, 904
TspGWI	ACGGA	3 cut(s)	669, 774, 1386
TspRI	CASTG	3 cut(s)	50, 394, 1251
Van91I	CCANNNNNTGG	1 cut(s)	222
VpaK11BI	GGWCC	3 cut(s)	286, 366, 1154
XapI	RAATTY	5 cut(s)	240, 334, 372, 472, 709
XceI	RCATGY	3 cut(s)	22, 59, 1063
XcmI	CCANNNNNNNNNTGG	1 cut(s)	962
XmaJI	CCTAGG	1 cut(s)	1157
XmiI	GTMKAC	1 cut(s)	1458
XspI	CTAG	4 cut(s)	26, 497, 624, 1158
ZraI	GACGTC	1 cut(s)	168
ZrmI	AGTACT	2 cut(s)	7, 515
Zsp2I	ATGCAT	1 cut(s)	232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.