MD07G1232100.v1.1

membrane protein At1g06890-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001213
Physical Location & Seq
Forward (+)
30621973 .. 30625743
3771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1232100.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGGGGAAGGCGAGAGGTTTCAGCTGGGGACAGTGGGAGCGTTAACCCTCTCTGTGGTCTCATCGGTCTCCATTGTCATTTGCAACAAGGCCCTCATCAGTTCCTTGGGTTTCACCTTTGCCACAACTTTGACAAGCTGGCATCTTTTAGTCACCTTTTGTTCCCTTCACGCGGCTTTAAAGATGAAATTCTTTGAACACAAACCCCTTGATCAAACGACTGTTACGGGGTTTGGCATTCTGAATGGAATCTCAATTGGACTGTTAAATCTGAGCCTGGGCTTCAATTCGGTTGGCTTTTATCAGATGACTAAGCTAGCAATCATCCCTTGCACTGTACTATTGGAGACCCTTTTCCTCGCGAAGAAATTCAGGAGCATTCAGATTGCCCTCCTTATTCTCCTCATTGGTGTTGGGATTGCAACAGTCACTGATGTTCAACTTAATGCTCTTGGAAGTATCTTGTCTCTTCTTGCAGTTATTACAACATGCGTTGCTCAAATTATGACCAATACCATCCAGAAGAAGTATAAGGTTTCTTCAACGCAACTCCTGTATCAATCATGTCCATATCAGTCAGGAACTTTATTAATCAGTGGCCCATTTCTGGATTGGTTCTTGACGAATCAAAATGTATTTGCTTTCAAGTATACAACTCAAGTGTTGGTTTTCATAATTATGTCCTGCCTGATCTCCGTCTCGGTAAATTTTAGCACATTTCTTGTAATTGGAAAGACATCTCCTGTCACCTATCAAGTTCTTGGACATCTAAAGACATGCCTTGTGCTGGCATTTGGTTATATTTTGCTTCATGATCCATTTGACTGGAGGAACATCCTCGGAATATTCGTCGCACTTGCTGGGATGGTTTTATATTCGTACTTTTGCACTCATGAGGGTCAGAAAAAGGTTGCTGAAGAAGCAACGCAGCCATCACAGGCAGGGGAAGGTGAGTCGGATCCTCTGGTGGCCGCGGAAACTGGAAGCGGGGTAGGAACTGATTCGACTCCCCAAATTGGCCCTGTATGGAAGTCGAACAAAGATTTGCATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

352

Amino Acids

37.99

Weight (kDa)

8.07

Isoelectric Point (pI)

27.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 21 - 293 3.5e-16 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 650
AccII CGCG 3 cut(s) 173, 362, 974
AciI CCGC 4 cut(s) 173, 972, 974, 987
AclWI GGATC 3 cut(s) 809, 953, 966
AcoI YGGCCR 1 cut(s) 969
AcsI RAATTY 3 cut(s) 188, 368, 706
AcuI CTGAAG 1 cut(s) 936
AfaI GTAC 2 cut(s) 339, 881
AfiI CCNNNNNNNGG 5 cut(s) 55, 172, 607, 787, 1016
AgsI TTSAA 5 cut(s) 197, 286, 440, 543, 646
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 3 cut(s) 25, 138, 316
AluI AGCT 3 cut(s) 25, 138, 316
Alw26I GTCTC 5 cut(s) 64, 73, 341, 471, 703
AlwI GGATC 3 cut(s) 809, 953, 966
AlwNI CAGNNNCTG 1 cut(s) 431
AoxI GGCC 4 cut(s) 90, 598, 969, 1018
ApeKI GCWGC 1 cut(s) 928
ApoI RAATTY 3 cut(s) 188, 368, 706
AseI ATTAAT 1 cut(s) 590
Asp700I GAANNNNTTC 2 cut(s) 368, 1000
AspS9I GGNCC 3 cut(s) 91, 599, 1019
AsuHPI GGTGA 4 cut(s) 106, 145, 739, 962
AsuNHI GCTAGC 1 cut(s) 316
BaeI ACNNNNGTAYC 2 cut(s) 539, 572
BamHI GGATCC 1 cut(s) 958
BbvI GCAGC 1 cut(s) 940
BccI CCATC 3 cut(s) 524, 859, 940
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 1 cut(s) 211
BcoDI GTCTC 5 cut(s) 64, 73, 341, 471, 703
BfaI CTAG 1 cut(s) 317
BisI GCNGC 3 cut(s) 174, 929, 972
BlsI GCNGC 3 cut(s) 175, 930, 973
Bme1390I CCNGG 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 91, 599, 1019
BmiI GGNNCC 1 cut(s) 960
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 151
BmtI GCTAGC 1 cut(s) 320
BpmI CTGGAG 1 cut(s) 847
BpuEI CTTGAG 1 cut(s) 642
BsaI GGTCTC 3 cut(s) 64, 73, 341
BsaJI CCNNGG 4 cut(s) 105, 277, 838, 972
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 172, 607, 787, 1016
Bse1I ACTGG 2 cut(s) 830, 985
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 4 cut(s) 105, 277, 838, 972
BseGI GGATG 4 cut(s) 324, 516, 834, 870
BseLI CCNNNNNNNGG 5 cut(s) 55, 172, 607, 787, 1016
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 2 cut(s) 830, 985
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 1 cut(s) 940
BseYI CCCAGC 2 cut(s) 25, 860
Bsh1236I CGCG 3 cut(s) 173, 362, 974
BshFI GGCC 4 cut(s) 92, 600, 971, 1020
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 5 cut(s) 55, 172, 607, 787, 1016
BsmAI GTCTC 5 cut(s) 64, 73, 341, 471, 703
BsmBI CGTCTC 1 cut(s) 703
BsmFI GGGAC 1 cut(s) 43
BsmI GAATGC 2 cut(s) 237, 378
BsnI GGCC 4 cut(s) 92, 600, 971, 1020
Bso31I GGTCTC 3 cut(s) 64, 73, 341
Bsp143I GATC 4 cut(s) 211, 690, 814, 958
Bsp68I TCGCGA 1 cut(s) 362
BspACI CCGC 4 cut(s) 173, 972, 974, 987
BspANI GGCC 4 cut(s) 92, 600, 971, 1020
BspCNI CTCAG 1 cut(s) 264
BspFNI CGCG 3 cut(s) 173, 362, 974
BspHI TCATGA 2 cut(s) 811, 892
BspLI GGNNCC 1 cut(s) 960
BspOI GCTAGC 1 cut(s) 320
BspPI GGATC 3 cut(s) 809, 953, 966
BspTNI GGTCTC 3 cut(s) 64, 73, 341
BsrI ACTGG 2 cut(s) 830, 985
BssECI CCNNGG 4 cut(s) 105, 277, 838, 972
BssMI GATC 4 cut(s) 211, 690, 814, 958
BssNAI GTATAC 1 cut(s) 651
BssT1I CCWWGG 1 cut(s) 105
Bst1107I GTATAC 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 5 cut(s) 34, 223, 264, 337, 427
Bst6I CTCTTC 1 cut(s) 474
BstC8I GCNNGC 4 cut(s) 140, 318, 789, 1050
BstDEI CTNAG 2 cut(s) 272, 312
BstDSI CCRYGG 1 cut(s) 972
BstF5I GGATG 4 cut(s) 324, 516, 834, 870
BstFNI CGCG 3 cut(s) 173, 362, 974
BstKTI GATC 4 cut(s) 214, 693, 817, 961
BstMAI GTCTC 5 cut(s) 64, 73, 341, 471, 703
BstMBI GATC 4 cut(s) 211, 690, 814, 958
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 3 cut(s) 492, 780, 1052
BstSCI CCNGG 1 cut(s) 276
BstUI CGCG 3 cut(s) 173, 362, 974
BstV1I GCAGC 1 cut(s) 940
BstX2I RGATCY 1 cut(s) 958
BstXI CCANNNNNNTGG 1 cut(s) 825
BstYI RGATCY 1 cut(s) 958
BstZ17I GTATAC 1 cut(s) 651
BsuRI GGCC 4 cut(s) 92, 600, 971, 1020
BtgI CCRYGG 1 cut(s) 972
BtsCI GGATG 4 cut(s) 324, 516, 834, 870
BtsIMutI CAGTG 4 cut(s) 39, 333, 429, 601
BtuMI TCGCGA 1 cut(s) 362
Cac8I GCNNGC 4 cut(s) 140, 318, 789, 1050
CaiI CAGNNNCTG 1 cut(s) 431
CciI TCATGA 2 cut(s) 811, 892
Cfr13I GGNCC 3 cut(s) 91, 599, 1019
Cfr42I CCGCGG 1 cut(s) 975
Csp6I GTAC 2 cut(s) 338, 880
CviAII CATG 6 cut(s) 489, 564, 777, 812, 893, 1049
CviQI GTAC 2 cut(s) 338, 880
DdeI CTNAG 2 cut(s) 272, 312
DpnI GATC 4 cut(s) 213, 692, 816, 960
DpnII GATC 4 cut(s) 211, 690, 814, 958
DraI TTTAAA 1 cut(s) 180
EaeI YGGCCR 1 cut(s) 969
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 105
Eco31I GGTCTC 3 cut(s) 64, 73, 341
Eco57I CTGAAG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 703
FaeI CATG 6 cut(s) 492, 567, 780, 815, 896, 1052
FaqI GGGAC 1 cut(s) 43
FatI CATG 6 cut(s) 488, 563, 776, 811, 892, 1048
FauI CCCGC 1 cut(s) 980
FbaI TGATCA 1 cut(s) 211
FblI GTMKAC 1 cut(s) 650
Fnu4HI GCNGC 3 cut(s) 174, 929, 972
FokI GGATG 4 cut(s) 311, 503, 821, 877
Fsp4HI GCNGC 3 cut(s) 174, 929, 972
FspBI CTAG 1 cut(s) 317
GluI GCNGC 3 cut(s) 174, 929, 972
GsaI CCCAGC 2 cut(s) 29, 864
GsuI CTGGAG 1 cut(s) 847
HaeIII GGCC 4 cut(s) 92, 600, 971, 1020
Hin1II CATG 6 cut(s) 492, 567, 780, 815, 896, 1052
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 5 cut(s) 249, 625, 953, 1001, 1006
HpaI GTTAAC 1 cut(s) 45
HphI GGTGA 4 cut(s) 106, 145, 739, 962
Hpy166II GTNNAC 2 cut(s) 45, 651
Hpy188I TCNGA 7 cut(s) 243, 273, 306, 384, 842, 903, 958
Hpy188III TCNNGA 8 cut(s) 361, 373, 520, 579, 608, 619, 812, 893
Hpy8I GTNNAC 2 cut(s) 45, 651
Hpy99I CGWCG 1 cut(s) 854
HpyAV CCTTC 2 cut(s) 176, 941
HpyCH4III ACNGT 5 cut(s) 34, 223, 264, 337, 427
HpyCH4V TGCA 6 cut(s) 84, 333, 422, 476, 888, 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 2 cut(s) 272, 312
Hsp92II CATG 6 cut(s) 492, 567, 780, 815, 896, 1052
Ksp22I TGATCA 1 cut(s) 211
KspAI GTTAAC 1 cut(s) 45
KspI CCGCGG 1 cut(s) 975
Kzo9I GATC 4 cut(s) 211, 690, 814, 958
LmnI GCTCC 2 cut(s) 38, 375
Lsp1109I GCAGC 1 cut(s) 940
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 317
MaeIII GTNAC 4 cut(s) 151, 223, 427, 745
MalI GATC 4 cut(s) 213, 692, 816, 960
MboI GATC 4 cut(s) 211, 690, 814, 958
MboII GAAGA 5 cut(s) 376, 461, 531, 535, 929
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 958
MluCI AATT 9 cut(s) 188, 255, 286, 368, 501, 675, 706, 726, 1014
MlyI GAGTC 2 cut(s) 962, 1000
MmeI TCCRAC 1 cut(s) 936
MroXI GAANNNNTTC 2 cut(s) 368, 1000
MseI TTAA 5 cut(s) 44, 179, 266, 444, 590
MslI CAYNNNNRTG 1 cut(s) 677
MspA1I CMGCKG 2 cut(s) 25, 974
MspR9I CCNGG 1 cut(s) 278
MunI CAATTG 1 cut(s) 255
Mva1269I GAATGC 2 cut(s) 237, 378
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 3 cut(s) 173, 362, 974
MwoI GCNNNNNNNGC 1 cut(s) 920
NdeII GATC 4 cut(s) 211, 690, 814, 958
NheI GCTAGC 1 cut(s) 316
NlaIII CATG 6 cut(s) 492, 567, 780, 815, 896, 1052
NlaIV GGNNCC 1 cut(s) 960
NmuCI GTSAC 3 cut(s) 151, 427, 745
NruI TCGCGA 1 cut(s) 362
NspI RCATGY 3 cut(s) 492, 780, 1052
PaeI GCATGC 1 cut(s) 1052
PagI TCATGA 2 cut(s) 811, 892
PcsI WCGNNNNNNNCGW 1 cut(s) 846
PctI GAATGC 2 cut(s) 237, 378
PdmI GAANNNNTTC 2 cut(s) 368, 1000
PfeI GAWTC 3 cut(s) 249, 625, 1001
PkrI GCNGC 3 cut(s) 175, 930, 973
PleI GAGTC 2 cut(s) 961, 1000
PpsI GAGTC 2 cut(s) 961, 1000
PshBI ATTAAT 1 cut(s) 590
Psp6I CCWGG 1 cut(s) 276
PspFI CCCAGC 2 cut(s) 25, 860
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 960
PspPI GGNCC 3 cut(s) 91, 599, 1019
PstNI CAGNNNCTG 1 cut(s) 431
PsuI RGATCY 1 cut(s) 958
PvuII CAGCTG 1 cut(s) 25
RruI TCGCGA 1 cut(s) 362
RsaI GTAC 2 cut(s) 339, 881
RsaNI GTAC 2 cut(s) 338, 880
RseI CAYNNNNRTG 1 cut(s) 677
SacII CCGCGG 1 cut(s) 975
SaqAI TTAA 5 cut(s) 44, 179, 266, 444, 590
SatI GCNGC 3 cut(s) 174, 929, 972
Sau3AI GATC 4 cut(s) 211, 690, 814, 958
Sau96I GGNCC 3 cut(s) 91, 599, 1019
SchI GAGTC 2 cut(s) 962, 1000
ScrFI CCNGG 1 cut(s) 278
SfaNI GCATC 1 cut(s) 151
Sfr303I CCGCGG 1 cut(s) 975
SgrBI CCGCGG 1 cut(s) 975
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
SphI GCATGC 1 cut(s) 1052
Sse9I AATT 9 cut(s) 188, 255, 286, 368, 501, 675, 706, 726, 1014
SsiI CCGC 4 cut(s) 173, 972, 974, 987
SspI AATATT 1 cut(s) 846
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 5 cut(s) 34, 223, 264, 337, 427
TaqI TCGA 2 cut(s) 1004, 1034
TaqII GACCGA 1 cut(s) 55
TasI AATT 9 cut(s) 188, 255, 286, 368, 501, 675, 706, 726, 1014
TatI WGTACW 1 cut(s) 337
TauI GCSGC 2 cut(s) 176, 974
TfiI GAWTC 3 cut(s) 249, 625, 1001
Tru1I TTAA 5 cut(s) 44, 179, 266, 444, 590
Tru9I TTAA 5 cut(s) 44, 179, 266, 444, 590
TscAI CASTG 4 cut(s) 39, 340, 436, 601
TseFI GTSAC 3 cut(s) 151, 427, 745
TseI GCWGC 1 cut(s) 928
Tsp45I GTSAC 3 cut(s) 151, 427, 745
TspDTI ATGAA 3 cut(s) 200, 661, 800
TspGWI ACGGA 1 cut(s) 685
TspRI CASTG 4 cut(s) 39, 340, 436, 601
VspI ATTAAT 1 cut(s) 590
XapI RAATTY 3 cut(s) 188, 368, 706
XceI RCATGY 3 cut(s) 492, 780, 1052
XcmI CCANNNNNNNNNTGG 1 cut(s) 609
XmiI GTMKAC 1 cut(s) 650
XmnI GAANNNNTTC 2 cut(s) 368, 1000
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.