MD07G1302300.v1.1

serine protease inhibitor, Kazal-type family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003543
Physical Location & Seq
Forward (+)
36069914 .. 36070336
423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1302300.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGAAGCCCATTTCGCAAAATCGGTGCCAGTCCCTCTCATCCTCCTGAGCCTCTTCCCAATCATTGTCTTCTGTTCCTTCACTACAGTTCGATCCGACCACGAATCATCTACGATGATTAATCTCCGGTTGCCCTCAGATGCCACGTCCGCGGATCGATGCAGTGCACAAGTGGAGTCACCCTTGTCCCGCCCCATGTGTCCGGTAAGCTGCTTTAGACCAGACCCGGTGTGTGGGGTGGACGGGGTTACCTACTGGTGTGGGTGCCAAGAGGCACAATGTGCCGGTGTCAAAGTAGGAAAATTAGGGTTTTGTGAAGTGGGCAACGGGGGGTCTGCCCCTCTCTCTGCTCAGGCGCTGCTTCTGGTTCACATTGTTTGGCTGATCGTCCTCGGCTTCGCGGTCCTTTTCGGGCTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

14.92

Weight (kDa)

5.82

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 25, 264
AccII CGCG 2 cut(s) 152, 401
AciI CCGC 4 cut(s) 150, 152, 190, 401
AclWI GGATC 2 cut(s) 87, 162
AdeI CACNNNGTG 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 233
AjiI CACGTC 1 cut(s) 147
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
Alw21I GWGCWC 1 cut(s) 169
Alw44I GTGCAC 1 cut(s) 165
AlwI GGATC 2 cut(s) 87, 162
AlwNI CAGNNNCTG 1 cut(s) 358
ApaLI GTGCAC 1 cut(s) 165
ApeKI GCWGC 2 cut(s) 210, 358
AseI ATTAAT 1 cut(s) 120
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 403
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 171
AvaII GGWCC 1 cut(s) 403
BaeGI GKGCMC 1 cut(s) 169
BanI GGYRCC 2 cut(s) 25, 264
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 2 cut(s) 197, 345
BcnI CCSGG 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 359
BisI GCNGC 2 cut(s) 211, 359
BlsI GCNGC 2 cut(s) 212, 360
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 403
BmgBI CACGTC 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 2 cut(s) 27, 266
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 130, 149
BpiI GAAGAC 1 cut(s) 61
Bpu10I CCTNAGC 2 cut(s) 47, 351
BpuMI CCSGG 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 150, 391
BsaWI WCCGGW 2 cut(s) 126, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 2 cut(s) 29, 260
BseCI ATCGAT 1 cut(s) 157
BseDI CCNNGG 2 cut(s) 150, 391
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 3 cut(s) 38, 150, 365
BseNI ACTGG 2 cut(s) 29, 260
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 2 cut(s) 197, 345
Bsh1236I CGCG 2 cut(s) 152, 401
BshNI GGYRCC 2 cut(s) 25, 264
BshVI ATCGAT 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 169
BsiSI CCGG 4 cut(s) 127, 203, 227, 285
BslFI GGGAC 2 cut(s) 17, 172
BslI CCNNNNNNNGG 1 cut(s) 233
BsmFI GGGAC 2 cut(s) 17, 172
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 3 cut(s) 92, 154, 384
BspACI CCGC 4 cut(s) 150, 152, 190, 401
BspCNI CTCAG 3 cut(s) 39, 149, 364
BspDI ATCGAT 1 cut(s) 157
BspFNI CGCG 2 cut(s) 152, 401
BspLI GGNNCC 2 cut(s) 27, 266
BspPI GGATC 2 cut(s) 87, 162
BspT107I GGYRCC 2 cut(s) 25, 264
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 2 cut(s) 29, 260
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 150, 391
BssMI GATC 3 cut(s) 92, 154, 384
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 281
BstDEI CTNAG 3 cut(s) 47, 136, 351
BstDSI CCRYGG 1 cut(s) 150
BstEII GGTNACC 1 cut(s) 247
BstF5I GGATG 1 cut(s) 39
BstFNI CGCG 2 cut(s) 152, 401
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 3 cut(s) 95, 157, 387
BstMBI GATC 3 cut(s) 92, 154, 384
BstMWI GCNNNNNNNGC 3 cut(s) 14, 149, 281
BstPI GGTNACC 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 84
BstSLI GKGCMC 1 cut(s) 169
BstUI CGCG 2 cut(s) 152, 401
BstV1I GCAGC 2 cut(s) 197, 345
BstV2I GAAGAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 157
BsuTUI ATCGAT 1 cut(s) 157
BtgI CCRYGG 1 cut(s) 150
BtrI CACGTC 1 cut(s) 147
BtsCI GGATG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 169
CaiI CAGNNNCTG 1 cut(s) 358
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 403
Cfr42I CCGCGG 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 157
CviAII CATG 1 cut(s) 196
CviJI RGCY 6 cut(s) 8, 51, 210, 382, 396, 415
CviKI_1 RGCY 6 cut(s) 8, 51, 210, 382, 396, 415
DdeI CTNAG 3 cut(s) 47, 136, 351
DpnI GATC 3 cut(s) 94, 156, 386
DpnII GATC 3 cut(s) 92, 154, 384
DraIII CACNNNGTG 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 403
Eco91I GGTNACC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 247
FaeI CATG 1 cut(s) 199
FaiI YATR 1 cut(s) 197
FaqI GGGAC 2 cut(s) 17, 172
FatI CATG 1 cut(s) 195
FauI CCCGC 1 cut(s) 197
Fnu4HI GCNGC 2 cut(s) 211, 359
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 2 cut(s) 211, 359
GlaI GCGC 1 cut(s) 357
GluI GCNGC 2 cut(s) 211, 359
HaeII RGCGCY 1 cut(s) 359
HapII CCGG 4 cut(s) 127, 203, 227, 285
HhaI GCGC 1 cut(s) 358
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 2 cut(s) 104, 176
HpaII CCGG 4 cut(s) 127, 203, 227, 285
HphI GGTGA 1 cut(s) 171
Hpy166II GTNNAC 3 cut(s) 167, 241, 370
Hpy188I TCNGA 3 cut(s) 97, 139, 422
Hpy188III TCNNGA 1 cut(s) 46
Hpy8I GTNNAC 3 cut(s) 167, 241, 370
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 2 cut(s) 162, 167
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 149, 281
HpyF3I CTNAG 3 cut(s) 47, 136, 351
HpySE526I ACGT 1 cut(s) 146
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 1 cut(s) 356
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 3 cut(s) 92, 154, 384
Lsp1109I GCAGC 2 cut(s) 197, 345
LweI GCATC 2 cut(s) 130, 149
MaeII ACGT 1 cut(s) 146
MaeIII GTNAC 2 cut(s) 177, 247
MalI GATC 3 cut(s) 94, 156, 386
MboI GATC 3 cut(s) 92, 154, 384
MboII GAAGA 2 cut(s) 46, 61
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 1 cut(s) 302
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 7 cut(s) 45, 53, 62, 145, 265, 351, 401
MseI TTAA 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 4 cut(s) 127, 203, 227, 285
MspR9I CCNGG 1 cut(s) 227
MvnI CGCG 2 cut(s) 152, 401
MwoI GCNNNNNNNGC 3 cut(s) 14, 149, 281
NciI CCSGG 1 cut(s) 227
NdeII GATC 3 cut(s) 92, 154, 384
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 2 cut(s) 27, 266
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 177
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 2 cut(s) 212, 360
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 27, 266
PspPI GGNCC 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 358
SacII CCGCGG 1 cut(s) 153
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 211, 359
Sau3AI GATC 3 cut(s) 92, 154, 384
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 1 cut(s) 169
SetI ASST 3 cut(s) 149, 212, 254
SfaNI GCATC 2 cut(s) 130, 149
SfcI CTRYAG 1 cut(s) 84
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 1 cut(s) 403
Sse9I AATT 1 cut(s) 302
SsiI CCGC 4 cut(s) 150, 152, 190, 401
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 1 cut(s) 149
TaqI TCGA 2 cut(s) 91, 157
TasI AATT 1 cut(s) 302
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 169
TseFI GTSAC 1 cut(s) 177
TseI GCWGC 2 cut(s) 210, 358
Tsp45I GTSAC 1 cut(s) 177
TspRI CASTG 1 cut(s) 169
VneI GTGCAC 1 cut(s) 165
VpaK11BI GGWCC 1 cut(s) 403
VspI ATTAAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.