Rorug02G0457700

serine protease inhibitor, Kazal-type family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003543
Physical Location & Seq
Forward (+)
58902004 .. 58905446
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0457700.1

Sequence Viewer

Length: 2031 bp
ATGGGCGCGCATATGGCTATGGCTAGGCTTATGCGCGCACCAAACCCATCATTCCTGCTCGCTCTCATTGTAGCTACGCTTTCTTTTATGAATATGTTGTCTTTTGGTTTGCCGGACACCACGTCCGACGCCCTGTTAAAATTCAAGAGTTCTTTGGGGAACAGTTCCGAAGCTCTTACGAATTGGGGTAGTTCTCCGAACCCATGCAATGGTAACAGGGGGAATTGGGTTGGTGTGCTTTGCTTTCAAGGGAATGTTAGGGGCTTGAAACTCGAAGGCATGGGGTTACAGGGAACGTTAGATGTGGAACCTCTCGCGTCGTTGCCATATTTGAAGACTTTAAGCTTCACGAACAATTCATTCATAGGTCCGTTGCCTAATCTCACTAGACTAAAGAGATTAGGGTCGTTATATTTGTCAGATAATCATTTGTCGGGGGATATTCCTGATGATGCATTTGAGAATTTGACTTTCTTGAAGAAACTGTATTTGGGAAATAATGAGTTCTCAGGCAAAATCCCTTCATCTCTCGCCAAATTGCCAAACCTTTTCGACGTGGGGCTTGAGAATAACTATTTTAGTGGCGAAATCCCTGATTTTAATAGTGGGGACTCAGAGAAATTATGGAGGTTGTATTTGGCTAACAATGAGTTGGAGGGAGAAATCCCAAAAAGCCTGAGAACGCAGGAGCCTAGCAGTTTTGCAGGAAATGAAAACCTATGTGGTCCGCCTATAGAAGCATGTAGCCCCTCTCCTGCTCCTGCTCCGTCTCCATCGCCATCTTCGCCTCCATGTGGGGGATCCGGGGAACCATGCAGCCCTTCCGACCCTCCTACGAAGTCTTCATCTCTTGGTCTAAAAATAGCCTTGGGTATAGTGAGTATTCTGCTCCTATTAGTTATCATTGCTGTACTTTTGATGTTCCTCAGAAAGAGAAAGCAACAACCGGAATTAGACCAGGCGGAGTTGTTGGATGAATCCTCCAAGTACACAGCAGCAACTGCGAGCCAGATGGATGCCAAGTCGGTAGAAAATAATAATGTGAACAATCCACCAAGGAGGGGTGAGCAAGCAGGAAAGCTGTCATTTGTGAGGGATGACAGGGACAGGTTTGATTTGCATGACCTGCTAAGAGCCTCAGCCGAAATATTGGGGAGTGGGAACTTTGGGGCTTCATACAAGGCTTTGATACTAACTGATGCTGTAGTGGTCAAGAGGTATAAGCAGATGAACAATGTGGGAAGAGAGGAGTTCCATGACCACATGAGAAGGCTTGGGAGATTGACACACCAAAACTTGTTGCCAATCGTGGCCTATTATTATAGAAGGGAAGAGAAGCTCTTGGTTTCTGACTTTGTCGAGAACGGTAGCTTGGCTTCTCATCTTCATGGCAATCACAATCTGCATAAGCCTGGACTTGATTGGCCAACCCGTTTGAGAATAATCAAAGGAATAGGAAGGGGCCTGACATACCTCTACAACACACTCCCAAGCTTGGTTGTACCTCATGGTCATCTGAAATCCTCCAATGTGCTTCTGGACGAAAACATGGAGCCTCTACTGAACGACTATGCTCTACTCCCCGTGATCAACATGGAACAAGCCCAACACCTCATGATGGCTTACAAGTCACCCGAATACGCGCGGCACCACCGGATAACAAAGAAGACCGACGTGTGGTGCTTCGGGATAATCATGTTGGAGGTGTTGACAGGCAAGTTCCCAGAAAACTATCTAAAGCAAAGTTATGACAGTAAGGCGGATTTGGCGAGCTGGGTGAACGGAATGATCAAGGAGAAGAAGACTAGTGAGGTGTTTGATGGAGAAATGGGGAATGTCGGTGACAGCAAAGGTGAACTTCTAAAGCTGTTGAAGATTGGAGTGAGTTGTTGTGAGGAGGATGTGGAGAGAAGGCTGGACTTGCATGAAGTTGTTGCAAAGATTGAGGAGTTGAATGAGGGAGAAAGTGATGGGGAGTACCGCTCAAGTGTTTCAAGTGAAGAAGATGATTATACTTCTCAGGCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

676

Amino Acids

74.66

Weight (kDa)

5.75

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 81 1.1e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 133 - 192 9.5e-09 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 382 - 647 1.3e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 382 - 646 1.1e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1134
AccB1I GGYRCC 1 cut(s) 1647
AccB7I CCANNNNNTGG 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 1983
AccII CGCG 5 cut(s) 8, 36, 317, 1643, 1645
AciI CCGC 5 cut(s) 728, 962, 1645, 1760, 1981
AclI AACGTT 1 cut(s) 296
AclWI GGATC 2 cut(s) 795, 808
AcoI YGGCCR 1 cut(s) 1424
AcsI RAATTY 2 cut(s) 140, 463
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 4 cut(s) 912, 989, 1503, 1979
AfiI CCNNNNNNNGG 7 cut(s) 209, 794, 797, 1061, 1495, 1618, 1677
AflIII ACRYGT 1 cut(s) 1674
AgsI TTSAA 8 cut(s) 145, 248, 268, 334, 478, 1873, 1954, 1995
AhdI GACNNNNNGTC 1 cut(s) 121
AhlI ACTAGT 1 cut(s) 1805
AjiI CACGTC 3 cut(s) 123, 556, 1675
AjnI CCWGG 2 cut(s) 957, 1411
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 1355, 1387
AluBI AGCT 9 cut(s) 74, 173, 345, 1081, 1339, 1371, 1494, 1773, 1867
AluI AGCT 9 cut(s) 74, 173, 345, 1081, 1339, 1371, 1494, 1773, 1867
Alw26I GTCTC 1 cut(s) 774
AlwI GGATC 2 cut(s) 795, 808
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 3 cut(s) 1311, 1424, 1462
ApeKI GCWGC 2 cut(s) 816, 995
ApoI RAATTY 2 cut(s) 140, 463
AspLEI GCGC 5 cut(s) 8, 10, 36, 38, 1645
AspS9I GGNCC 3 cut(s) 368, 725, 1462
AsuC2I CCSGG 1 cut(s) 805
AsuHPI GGTGA 5 cut(s) 1076, 1623, 1789, 1853, 1865
AvaII GGWCC 2 cut(s) 368, 725
BalI TGGCCA 1 cut(s) 1426
BamHI GGATCC 1 cut(s) 800
BanI GGYRCC 1 cut(s) 1647
BarI GAAGNNNNNNTAC 2 cut(s) 1360, 1392
BbsI GAAGAC 4 cut(s) 341, 834, 1673, 1808
BbvCI CCTCAGC 1 cut(s) 1138
BbvI GCAGC 2 cut(s) 828, 1007
BccI CCATC 7 cut(s) 55, 781, 787, 1006, 1612, 1814, 1964
BciT130I CCWGG 2 cut(s) 959, 1413
BclI TGATCA 2 cut(s) 1587, 1788
BcnI CCSGG 1 cut(s) 805
BcoDI GTCTC 1 cut(s) 774
BcuI ACTAGT 1 cut(s) 1805
BfaI CTAG 4 cut(s) 24, 387, 693, 1806
BfmI CTRYAG 2 cut(s) 732, 1203
BfuAI ACCTGC 1 cut(s) 1134
BisI GCNGC 3 cut(s) 817, 996, 1646
BlsI GCNGC 3 cut(s) 818, 997, 1647
Bme1390I CCNGG 3 cut(s) 805, 959, 1413
Bme18I GGWCC 2 cut(s) 368, 725
BmeRI GACNNNNNGTC 1 cut(s) 121
BmgBI CACGTC 3 cut(s) 123, 556, 1675
BmgT120I GGNCC 3 cut(s) 368, 725, 1462
BmiI GGNNCC 7 cut(s) 309, 690, 802, 810, 1463, 1554, 1649
BmrFI CCNGG 3 cut(s) 805, 959, 1413
BmsI GCATC 3 cut(s) 442, 1006, 1189
BpiI GAAGAC 4 cut(s) 341, 834, 1673, 1808
BplI GAGNNNNNCTC 2 cut(s) 1967, 1999
Bpu10I CCTNAGC 1 cut(s) 1138
BpuEI CTTGAG 2 cut(s) 584, 1969
BpuMI CCSGG 1 cut(s) 805
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 3 cut(s) 804, 867, 1055
BsaWI WCCGGW 2 cut(s) 946, 1653
Bsc4I CCNNNNNNNGG 7 cut(s) 209, 794, 797, 1061, 1495, 1618, 1677
Bse3DI GCAATG 2 cut(s) 214, 903
BseBI CCWGG 2 cut(s) 959, 1413
BseDI CCNNGG 3 cut(s) 804, 867, 1055
BseGI GGATG 4 cut(s) 979, 1021, 1102, 1906
BseLI CCNNNNNNNGG 7 cut(s) 209, 794, 797, 1061, 1495, 1618, 1677
BseMI GCAATG 2 cut(s) 214, 903
BseMII CTCAG 5 cut(s) 522, 627, 668, 940, 1152
BsePI GCGCGC 2 cut(s) 6, 34
BseRI GAGGAG 3 cut(s) 1262, 1910, 1961
BseXI GCAGC 2 cut(s) 828, 1007
BseYI CCCAGC 1 cut(s) 1773
Bsh1236I CGCG 5 cut(s) 8, 36, 317, 1643, 1645
BshFI GGCC 3 cut(s) 1313, 1426, 1464
BshNI GGYRCC 1 cut(s) 1647
BsiSI CCGG 4 cut(s) 113, 804, 947, 1654
BslFI GGGAC 2 cut(s) 623, 1118
BslI CCNNNNNNNGG 7 cut(s) 209, 794, 797, 1061, 1495, 1618, 1677
BsmAI GTCTC 1 cut(s) 774
BsmBI CGTCTC 1 cut(s) 774
BsmFI GGGAC 2 cut(s) 623, 1118
BsnI GGCC 3 cut(s) 1313, 1426, 1464
Bsp143I GATC 3 cut(s) 800, 1587, 1788
BspACI CCGC 5 cut(s) 728, 962, 1645, 1760, 1981
BspANI GGCC 3 cut(s) 1313, 1426, 1464
BspCNI CTCAG 5 cut(s) 521, 626, 669, 939, 1151
BspFNI CGCG 5 cut(s) 8, 36, 317, 1643, 1645
BspHI TCATGA 1 cut(s) 1614
BspLI GGNNCC 7 cut(s) 309, 690, 802, 810, 1463, 1554, 1649
BspMI ACCTGC 1 cut(s) 1134
BspPI GGATC 2 cut(s) 795, 808
BspT107I GGYRCC 1 cut(s) 1647
BsrBI CCGCTC 1 cut(s) 1983
BsrDI GCAATG 2 cut(s) 214, 903
BssECI CCNNGG 3 cut(s) 804, 867, 1055
BssHII GCGCGC 2 cut(s) 6, 34
BssMI GATC 3 cut(s) 800, 1587, 1788
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 2 cut(s) 867, 1055
Bst2UI CCWGG 2 cut(s) 959, 1413
Bst4CI ACNGT 4 cut(s) 164, 486, 1367, 1754
Bst6I CTCTTC 2 cut(s) 1237, 1326
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 2 cut(s) 1001, 1126
BstC8I GCNNGC 6 cut(s) 8, 36, 60, 1006, 1071, 1771
BstDEI CTNAG 7 cut(s) 508, 613, 677, 926, 1130, 1138, 2019
BstF5I GGATG 4 cut(s) 979, 1021, 1102, 1906
BstFNI CGCG 5 cut(s) 8, 36, 317, 1643, 1645
BstHHI GCGC 5 cut(s) 8, 10, 36, 38, 1645
BstKTI GATC 3 cut(s) 803, 1590, 1791
BstMAI GTCTC 1 cut(s) 774
BstMBI GATC 3 cut(s) 800, 1587, 1788
BstMWI GCNNNNNNNGC 6 cut(s) 14, 784, 1001, 1126, 1766, 1921
BstNI CCWGG 2 cut(s) 959, 1413
BstNSI RCATGY 1 cut(s) 744
BstSCI CCNGG 3 cut(s) 803, 957, 1411
BstSFI CTRYAG 2 cut(s) 732, 1203
BstUI CGCG 5 cut(s) 8, 36, 317, 1643, 1645
BstV1I GCAGC 2 cut(s) 828, 1007
BstV2I GAAGAC 4 cut(s) 341, 834, 1673, 1808
BstX2I RGATCY 1 cut(s) 800
BstYI RGATCY 1 cut(s) 800
BsuRI GGCC 3 cut(s) 1313, 1426, 1464
BtgZI GCGATG 1 cut(s) 759
BtrI CACGTC 3 cut(s) 123, 556, 1675
BtsCI GGATG 4 cut(s) 979, 1021, 1102, 1906
BveI ACCTGC 1 cut(s) 1134
Cac8I GCNNGC 6 cut(s) 8, 36, 60, 1006, 1071, 1771
CaiI CAGNNNCTG 1 cut(s) 1001
CciI TCATGA 1 cut(s) 1614
CfoI GCGC 5 cut(s) 8, 10, 36, 38, 1645
Cfr13I GGNCC 3 cut(s) 368, 725, 1462
CseI GACGC 2 cut(s) 137, 306
Csp6I GTAC 4 cut(s) 911, 988, 1502, 1978
CviQI GTAC 4 cut(s) 911, 988, 1502, 1978
DdeI CTNAG 7 cut(s) 508, 613, 677, 926, 1130, 1138, 2019
DpnI GATC 3 cut(s) 802, 1589, 1790
DpnII GATC 3 cut(s) 800, 1587, 1788
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 1424
Eam1104I CTCTTC 2 cut(s) 1237, 1326
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 2 cut(s) 1237, 1326
EciI GGCGGA 3 cut(s) 717, 977, 1775
Eco130I CCWWGG 2 cut(s) 867, 1055
Eco47I GGWCC 2 cut(s) 368, 725
EcoO109I RGGNCCY 1 cut(s) 1462
EcoRII CCWGG 2 cut(s) 957, 1411
EcoT14I CCWWGG 2 cut(s) 867, 1055
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 867, 1055
Esp3I CGTCTC 1 cut(s) 774
FalI AAGNNNNNCTT 2 cut(s) 1842, 1874
FaqI GGGAC 2 cut(s) 623, 1118
FauNDI CATATG 1 cut(s) 12
FbaI TGATCA 2 cut(s) 1587, 1788
Fnu4HI GCNGC 3 cut(s) 817, 996, 1646
FokI GGATG 4 cut(s) 986, 1028, 1109, 1913
Fsp4HI GCNGC 3 cut(s) 817, 996, 1646
FspBI CTAG 4 cut(s) 24, 387, 693, 1806
GlaI GCGC 5 cut(s) 7, 9, 35, 37, 1644
GluI GCNGC 3 cut(s) 817, 996, 1646
GsaI CCCAGC 1 cut(s) 1777
HaeIII GGCC 3 cut(s) 1313, 1426, 1464
HapII CCGG 4 cut(s) 113, 804, 947, 1654
HgaI GACGC 2 cut(s) 137, 306
HhaI GCGC 5 cut(s) 8, 10, 36, 38, 1645
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 5 cut(s) 6, 8, 34, 36, 1643
HinP1I GCGC 5 cut(s) 6, 8, 34, 36, 1643
HincII GTYRAC 1 cut(s) 1710
HindII GTYRAC 1 cut(s) 1710
HindIII AAGCTT 2 cut(s) 343, 1492
HinfI GANTC 2 cut(s) 611, 977
HpaII CCGG 4 cut(s) 113, 804, 947, 1654
HphI GGTGA 5 cut(s) 1076, 1623, 1789, 1853, 1865
Hpy166II GTNNAC 5 cut(s) 990, 1045, 1710, 1780, 1856
Hpy188I TCNGA 9 cut(s) 127, 169, 198, 421, 616, 826, 929, 1351, 1518
Hpy188III TCNNGA 9 cut(s) 145, 349, 446, 475, 1213, 1360, 1538, 1615, 1687
Hpy8I GTNNAC 5 cut(s) 990, 1045, 1710, 1780, 1856
Hpy99I CGWCG 4 cut(s) 131, 322, 557, 1676
HpyAV CCTTC 7 cut(s) 269, 531, 831, 1263, 1320, 1452, 1905
HpyCH4III ACNGT 4 cut(s) 164, 486, 1367, 1754
HpyCH4IV ACGT 4 cut(s) 122, 296, 555, 1674
HpyCH4V TGCA 8 cut(s) 207, 455, 704, 816, 1120, 1405, 1924, 1937
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 784, 1001, 1126, 1766, 1921
HpyF3I CTNAG 7 cut(s) 508, 613, 677, 926, 1130, 1138, 2019
HpySE526I ACGT 4 cut(s) 122, 296, 555, 1674
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 5 cut(s) 6, 8, 34, 36, 1643
Ksp22I TGATCA 2 cut(s) 1587, 1788
Kzo9I GATC 3 cut(s) 800, 1587, 1788
LmnI GCTCC 5 cut(s) 688, 763, 769, 894, 1552
Lsp1109I GCAGC 2 cut(s) 828, 1007
LweI GCATC 3 cut(s) 442, 1006, 1189
MaeI CTAG 4 cut(s) 24, 387, 693, 1806
MaeII ACGT 4 cut(s) 122, 296, 555, 1674
MaeIII GTNAC 4 cut(s) 212, 285, 1629, 1841
MalI GATC 3 cut(s) 802, 1589, 1790
MbiI CCGCTC 1 cut(s) 1983
MboI GATC 3 cut(s) 800, 1587, 1788
MflI RGATCY 1 cut(s) 800
MlsI TGGCCA 1 cut(s) 1426
MluCI AATT 8 cut(s) 140, 181, 223, 355, 463, 536, 620, 950
MluNI TGGCCA 1 cut(s) 1426
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 5 cut(s) 150, 633, 849, 951, 1680
Mox20I TGGCCA 1 cut(s) 1426
Mph1103I ATGCAT 1 cut(s) 457
MscI TGGCCA 1 cut(s) 1426
MseI TTAA 3 cut(s) 137, 341, 600
MslI CAYNNNNRTG 1 cut(s) 1386
Msp20I TGGCCA 1 cut(s) 1426
MspI CCGG 4 cut(s) 113, 804, 947, 1654
MspR9I CCNGG 3 cut(s) 805, 959, 1413
MvaI CCWGG 2 cut(s) 959, 1413
MvnI CGCG 5 cut(s) 8, 36, 317, 1643, 1645
MwoI GCNNNNNNNGC 6 cut(s) 14, 784, 1001, 1126, 1766, 1921
NciI CCSGG 1 cut(s) 805
NdeI CATATG 1 cut(s) 12
NdeII GATC 3 cut(s) 800, 1587, 1788
NlaIV GGNNCC 7 cut(s) 309, 690, 802, 810, 1463, 1554, 1649
NmuCI GTSAC 2 cut(s) 1629, 1841
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 744
PagI TCATGA 1 cut(s) 1614
PauI GCGCGC 2 cut(s) 6, 34
PfeI GAWTC 1 cut(s) 977
PflFI GACNNNGTC 1 cut(s) 1355
PflMI CCANNNNNTGG 1 cut(s) 209
PkrI GCNGC 3 cut(s) 818, 997, 1647
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
Psp1406I AACGTT 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 957, 1411
PspFI CCCAGC 1 cut(s) 1773
PspGI CCWGG 2 cut(s) 957, 1411
PspN4I GGNNCC 7 cut(s) 309, 690, 802, 810, 1463, 1554, 1649
PspPI GGNCC 3 cut(s) 368, 725, 1462
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 800
PsyI GACNNNGTC 1 cut(s) 1355
PteI GCGCGC 2 cut(s) 6, 34
RsaI GTAC 4 cut(s) 912, 989, 1503, 1979
RsaNI GTAC 4 cut(s) 911, 988, 1502, 1978
RseI CAYNNNNRTG 1 cut(s) 1386
SaqAI TTAA 3 cut(s) 137, 341, 600
SatI GCNGC 3 cut(s) 817, 996, 1646
Sau3AI GATC 3 cut(s) 800, 1587, 1788
Sau96I GGNCC 3 cut(s) 368, 725, 1462
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 3 cut(s) 805, 959, 1413
SfaNI GCATC 3 cut(s) 442, 1006, 1189
SfcI CTRYAG 2 cut(s) 732, 1203
SinI GGWCC 2 cut(s) 368, 725
SmiMI CAYNNNNRTG 1 cut(s) 1386
SmlI CTYRAG 2 cut(s) 563, 1984
SmoI CTYRAG 2 cut(s) 563, 1984
SpeI ACTAGT 1 cut(s) 1805
Sse9I AATT 8 cut(s) 140, 181, 223, 355, 463, 536, 620, 950
SsiI CCGC 5 cut(s) 728, 962, 1645, 1760, 1981
SspI AATATT 1 cut(s) 1149
SspMI CTAG 4 cut(s) 24, 387, 693, 1806
StyD4I CCNGG 3 cut(s) 803, 957, 1411
StyI CCWWGG 2 cut(s) 867, 1055
TaaI ACNGT 4 cut(s) 164, 486, 1367, 1754
TaiI ACGT 4 cut(s) 125, 299, 558, 1677
TaqI TCGA 3 cut(s) 273, 552, 1359
TaqII GACCGA 1 cut(s) 1685
TasI AATT 8 cut(s) 140, 181, 223, 355, 463, 536, 620, 950
TatI WGTACW 2 cut(s) 910, 987
TauI GCSGC 1 cut(s) 1648
TfiI GAWTC 1 cut(s) 977
Tru1I TTAA 3 cut(s) 137, 341, 600
Tru9I TTAA 3 cut(s) 137, 341, 600
TseFI GTSAC 2 cut(s) 1629, 1841
TseI GCWGC 2 cut(s) 816, 995
Tsp45I GTSAC 2 cut(s) 1629, 1841
TspGWI ACGGA 3 cut(s) 360, 756, 1797
Tth111I GACNNNGTC 1 cut(s) 1355
Van91I CCANNNNNTGG 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 368, 725
XapI RAATTY 2 cut(s) 140, 463
XceI RCATGY 1 cut(s) 744
XcmI CCANNNNNNNNNTGG 1 cut(s) 1534
XspI CTAG 4 cut(s) 24, 387, 693, 1806
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.