Rorug01G0439700

serine protease inhibitor, Kazal-type family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003543
Physical Location & Seq
Reverse (-)
53839017 .. 53840937
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0439700.1

Sequence Viewer

Length: 864 bp
ATGGCTCCTCCTCCGCCTACGGTGGAGCAGAAAGGCGAGTCCACCTCCGGCGAGTCGACGCAGCGAGGCTCTCCGACGCCGTTTCTGAGCAAAACGTACCAGCTCGTCGACGATCCCGCCATCGATGACGTCATCGCCTGGAGCGGCGACGGATCCAGCTTCGTGGTGAAGAACCCCACCGTGTTCGCCAGAGATTTGCTTCCCAAGTATTTCAAGCACAACAACTTCTCCAGCTTCGTCCGGCAGCTCAACACCTACGGATTTCGGAAGATTATACCTGACCGGTGGGAATTCTCCAACGACTGCTTCCGCAGAGGCGAGAAGCGGCTGCTCTGCGACATACAGCGGCGGAGAATTATGCCTCCGGCGGCCGTGATTCCGGCGAAACCTATCACCTCTCCGGCGAACTCCGGCGAGGAGCAGGTGACCACTTCGTCGAGCTCCTCGCCGCGGACGAGGATTCCGTGGCAGCTGGTGGAGGAGAACGAGAGGCTGAGGAAGGAGAACCTCCGGCTGACCAAGGAGCTGGCGGAGATCAAGTCTCTCTGCCACAACATCTTCTCCCTGGTGTCGAATTACGCCTGTAATAAGCCACCGGAGGGCGGTTTAGGTGAGTTGACGCAGCTGAGTTTGATGCCCGGGGAGCGGTTTTCCGGCGGGGAGACGAGTCCGAAGATTTTCGGGGTGGAAATCGGAGCGAAGCGGGCGAGGGAGGAAGGCGGTGGAGGCGATGAGGAGACGGATCTACGGCTGCGGCAACCGAGCGGTGGTGGCGGTGTGAAATCAGAGCCGTCGGATGTGGAGCATCGTGAGAAGCCGTGGCTGAAGCAGTGCCACAGGGCTAATCAGAGGGTGTGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

287

Amino Acids

32.0

Weight (kDa)

8.7

Isoelectric Point (pI)

61.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 28 - 117 3.7e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 412
AasI GACNNNNNNGTC 1 cut(s) 433
AatII GACGTC 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 412
AccBSI CCGCTC 3 cut(s) 144, 646, 765
AccI GTMKAC 2 cut(s) 56, 108
AccII CGCG 1 cut(s) 451
AclWI GGATC 4 cut(s) 107, 147, 160, 750
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 845
AcyI GRCGYC 2 cut(s) 77, 129
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 5 cut(s) 450, 599, 602, 645, 767
AgeI ACCGGT 1 cut(s) 282
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 2 cut(s) 137, 564
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 8 cut(s) 103, 159, 234, 247, 441, 472, 526, 625
AluI AGCT 8 cut(s) 103, 159, 234, 247, 441, 472, 526, 625
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 3 cut(s) 546, 656, 731
AlwI GGATC 4 cut(s) 107, 147, 160, 750
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 6 cut(s) 61, 244, 328, 469, 622, 751
ApoI RAATTY 1 cut(s) 290
AsiGI ACCGGT 1 cut(s) 282
Asp700I GAANNNNTTC 1 cut(s) 677
AsuC2I CCSGG 2 cut(s) 639, 640
AsuHPI GGTGA 4 cut(s) 178, 385, 436, 623
AvaI CYCGRG 1 cut(s) 638
BamHI GGATCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 443
BbvCI CCTCAGC 1 cut(s) 494
BbvI GCAGC 6 cut(s) 73, 256, 315, 481, 634, 738
BccI CCATC 1 cut(s) 128
BceAI ACGGC 5 cut(s) 64, 356, 764, 775, 802
BciT130I CCWGG 2 cut(s) 139, 566
BcnI CCSGG 2 cut(s) 639, 640
BcoDI GTCTC 3 cut(s) 546, 656, 731
BfuAI ACCTGC 1 cut(s) 412
Bme1390I CCNGG 4 cut(s) 139, 566, 639, 640
BmeT110I CYCGRG 1 cut(s) 638
BmiI GGNNCC 2 cut(s) 6, 154
BmrFI CCNGG 4 cut(s) 139, 566, 639, 640
BmsI GCATC 2 cut(s) 624, 814
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 2 cut(s) 160, 214
Bpu10I CCTNAGC 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 639, 640
Bsa29I ATCGAT 1 cut(s) 123
BsaHI GRCGYC 2 cut(s) 77, 129
BsaJI CCNNGG 7 cut(s) 449, 464, 519, 564, 638, 639, 818
BsaWI WCCGGW 2 cut(s) 282, 595
BsaXI ACNNNNNCTCC 6 cut(s) 212, 242, 524, 545, 554, 575
Bsc4I CCNNNNNNNGG 5 cut(s) 450, 599, 602, 645, 767
Bse118I RCCGGY 1 cut(s) 282
BseBI CCWGG 2 cut(s) 139, 566
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 7 cut(s) 449, 464, 519, 564, 638, 639, 818
BseGI GGATG 1 cut(s) 802
BseLI CCNNNNNNNGG 5 cut(s) 450, 599, 602, 645, 767
BseMII CTCAG 3 cut(s) 77, 485, 617
BseRI GAGGAG 4 cut(s) 431, 433, 494, 749
BseX3I CGGCCG 1 cut(s) 369
BseXI GCAGC 6 cut(s) 73, 256, 315, 481, 634, 738
Bsh1236I CGCG 1 cut(s) 451
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 371
BshTI ACCGGT 1 cut(s) 282
BshVI ATCGAT 1 cut(s) 123
BsiEI CGRYCG 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 638
BslI CCNNNNNNNGG 5 cut(s) 450, 599, 602, 645, 767
BsmAI GTCTC 3 cut(s) 546, 656, 731
BsmBI CGTCTC 2 cut(s) 656, 731
BsnI GGCC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 638
Bsp1286I GDGCHC 1 cut(s) 443
Bsp143I GATC 4 cut(s) 112, 152, 534, 742
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 3 cut(s) 78, 486, 618
BspDI ATCGAT 1 cut(s) 123
BspFNI CGCG 1 cut(s) 451
BspLI GGNNCC 2 cut(s) 6, 154
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 4 cut(s) 107, 147, 160, 750
BsrBI CCGCTC 3 cut(s) 144, 646, 765
BsrFI RCCGGY 1 cut(s) 282
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 7 cut(s) 449, 464, 519, 564, 638, 639, 818
BssMI GATC 4 cut(s) 112, 152, 534, 742
BssNI GRCGYC 2 cut(s) 77, 129
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 2 cut(s) 139, 566
Bst4CI ACNGT 2 cut(s) 22, 181
BstACI GRCGYC 2 cut(s) 77, 129
BstC8I GCNNGC 2 cut(s) 528, 705
BstDEI CTNAG 3 cut(s) 86, 494, 626
BstDSI CCRYGG 3 cut(s) 449, 464, 818
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 1 cut(s) 802
BstFNI CGCG 1 cut(s) 451
BstKTI GATC 4 cut(s) 115, 155, 537, 745
BstMAI GTCTC 3 cut(s) 546, 656, 731
BstMBI GATC 4 cut(s) 112, 152, 534, 742
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 4 cut(s) 643, 704, 726, 771
BstNI CCWGG 2 cut(s) 139, 566
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 4 cut(s) 137, 564, 637, 638
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 6 cut(s) 73, 256, 315, 481, 634, 738
BstX2I RGATCY 2 cut(s) 152, 742
BstXI CCANNNNNNTGG 2 cut(s) 163, 526
BstYI RGATCY 2 cut(s) 152, 742
BstZI CGGCCG 1 cut(s) 369
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 1 cut(s) 371
BsuTUI ATCGAT 1 cut(s) 123
BtgI CCRYGG 3 cut(s) 449, 464, 818
BtgZI GCGATG 2 cut(s) 118, 744
BtsCI GGATG 1 cut(s) 802
BtsI GCAGTG 1 cut(s) 836
BtsIMutI CAGTG 1 cut(s) 836
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 528, 705
Cfr10I RCCGGY 1 cut(s) 282
Cfr42I CCGCGG 1 cut(s) 452
Cfr9I CCCGGG 1 cut(s) 638
ClaI ATCGAT 1 cut(s) 123
CseI GACGC 3 cut(s) 67, 85, 628
Csp6I GTAC 1 cut(s) 97
CspAI ACCGGT 1 cut(s) 282
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 3 cut(s) 86, 494, 626
DpnI GATC 4 cut(s) 114, 154, 536, 744
DpnII GATC 4 cut(s) 112, 152, 534, 742
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 369
EagI CGGCCG 1 cut(s) 369
EciI GGCGGA 2 cut(s) 364, 545
Ecl136II GAGCTC 1 cut(s) 441
EclXI CGGCCG 1 cut(s) 369
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 443
Eco52I CGGCCG 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 845
Eco88I CYCGRG 1 cut(s) 638
Eco91I GGTNACC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 441
EcoO65I GGTNACC 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 2 cut(s) 137, 564
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 519
Esp3I CGTCTC 2 cut(s) 656, 731
FaiI YATR 3 cut(s) 275, 341, 359
FauI CCCGC 3 cut(s) 124, 650, 696
FblI GTMKAC 2 cut(s) 56, 108
FokI GGATG 1 cut(s) 809
FriOI GRGCYC 1 cut(s) 443
GsuI CTGGAG 2 cut(s) 160, 214
HaeIII GGCC 1 cut(s) 371
HgaI GACGC 3 cut(s) 67, 85, 628
Hin1I GRCGYC 2 cut(s) 77, 129
HincII GTYRAC 3 cut(s) 57, 109, 618
HindII GTYRAC 3 cut(s) 57, 109, 618
HinfI GANTC 5 cut(s) 38, 53, 376, 460, 667
HphI GGTGA 4 cut(s) 178, 385, 436, 623
Hpy166II GTNNAC 4 cut(s) 42, 57, 109, 618
Hpy188I TCNGA 8 cut(s) 75, 87, 267, 672, 695, 787, 796, 849
Hpy188III TCNNGA 1 cut(s) 809
Hpy8I GTNNAC 4 cut(s) 42, 57, 109, 618
Hpy99I CGWCG 7 cut(s) 61, 79, 110, 113, 152, 439, 796
HpyAV CCTTC 2 cut(s) 493, 710
HpyCH4III ACNGT 2 cut(s) 22, 181
HpyCH4IV ACGT 2 cut(s) 95, 129
HpyF10VI GCNNNNNNNGC 4 cut(s) 643, 704, 726, 771
HpyF3I CTNAG 3 cut(s) 86, 494, 626
HpySE526I ACGT 2 cut(s) 95, 129
Hsp92I GRCGYC 2 cut(s) 77, 129
KspI CCGCGG 1 cut(s) 452
Kzo9I GATC 4 cut(s) 112, 152, 534, 742
LmnI GCTCC 9 cut(s) 10, 25, 141, 418, 446, 523, 643, 695, 802
Lsp1109I GCAGC 6 cut(s) 73, 256, 315, 481, 634, 738
LweI GCATC 2 cut(s) 624, 814
MaeII ACGT 2 cut(s) 95, 129
MaeIII GTNAC 1 cut(s) 424
MalI GATC 4 cut(s) 114, 154, 536, 744
MbiI CCGCTC 3 cut(s) 144, 646, 765
MboI GATC 4 cut(s) 112, 152, 534, 742
MboII GAAGA 4 cut(s) 181, 280, 550, 685
MflI RGATCY 2 cut(s) 152, 742
MhlI GDGCHC 1 cut(s) 443
MluCI AATT 4 cut(s) 290, 354, 574, 859
MlyI GAGTC 3 cut(s) 47, 62, 676
MmeI TCCRAC 3 cut(s) 98, 321, 774
MroXI GAANNNNTTC 1 cut(s) 677
MspA1I CMGCKG 4 cut(s) 346, 451, 472, 625
MspR9I CCNGG 4 cut(s) 139, 566, 639, 640
MvaI CCWGG 2 cut(s) 139, 566
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 4 cut(s) 643, 704, 726, 771
NciI CCSGG 2 cut(s) 639, 640
NdeII GATC 4 cut(s) 112, 152, 534, 742
NlaIV GGNNCC 2 cut(s) 6, 154
NmuCI GTSAC 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 412
PcsI WCGNNNNNNNCGW 2 cut(s) 452, 461
PdmI GAANNNNTTC 1 cut(s) 677
PfeI GAWTC 2 cut(s) 376, 460
PinAI ACCGGT 1 cut(s) 282
PleI GAGTC 3 cut(s) 46, 61, 675
PpsI GAGTC 3 cut(s) 46, 61, 675
Psp124BI GAGCTC 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 137, 564
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 137, 564
PspN4I GGNNCC 2 cut(s) 6, 154
PsuI RGATCY 2 cut(s) 152, 742
PvuII CAGCTG 2 cut(s) 472, 625
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SacI GAGCTC 1 cut(s) 443
SacII CCGCGG 1 cut(s) 452
SalI GTCGAC 2 cut(s) 55, 107
Sau3AI GATC 4 cut(s) 112, 152, 534, 742
SchI GAGTC 3 cut(s) 47, 62, 676
ScrFI CCNGG 4 cut(s) 139, 566, 639, 640
SduI GDGCHC 1 cut(s) 443
SfaNI GCATC 2 cut(s) 624, 814
Sfr303I CCGCGG 1 cut(s) 452
SgrBI CCGCGG 1 cut(s) 452
SgrDI CGTCGACG 1 cut(s) 107
SmaI CCCGGG 1 cut(s) 640
Sse9I AATT 4 cut(s) 290, 354, 574, 859
SstI GAGCTC 1 cut(s) 443
StyD4I CCNGG 4 cut(s) 137, 564, 637, 638
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 2 cut(s) 22, 181
TaiI ACGT 2 cut(s) 98, 132
TaqI TCGA 5 cut(s) 56, 108, 123, 437, 572
TasI AATT 4 cut(s) 290, 354, 574, 859
TauI GCSGC 6 cut(s) 147, 328, 349, 371, 451, 757
TfiI GAWTC 2 cut(s) 376, 460
TscAI CASTG 1 cut(s) 836
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 6 cut(s) 61, 244, 328, 469, 622, 751
Tsp45I GTSAC 1 cut(s) 424
TspGWI ACGGA 4 cut(s) 165, 273, 453, 755
TspMI CCCGGG 1 cut(s) 638
TspRI CASTG 1 cut(s) 836
XapI RAATTY 1 cut(s) 290
XmaI CCCGGG 1 cut(s) 638
XmiI GTMKAC 2 cut(s) 56, 108
XmnI GAANNNNTTC 1 cut(s) 677
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.