MD07G1309800.v1.1

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005922
Physical Location & Seq
Forward (+)
36485860 .. 36487071
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1309800.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGCAGCATCATCTTCCCTCCCAGTGGTGGGTAGTCGCCTGGGGAACCGGCGGCTTGCAGCTCTCGCTCTCTGCCTTGTATTGCTCATCGATATTACTACCACTACTTTGGTATTGGCTTCCAACAAGGGAAGAGGCCGCGGTGCAAATAAAGGTGCTACAAAGAAAGGTGGTGGTGCACACAGAGGTGGTGGTGCACACAAAGGTGGTGGTGCACACAAAGGTAGCGGTGCACACAGAGGTGGCGGTGCACAAAAAGGTGGCGGTCGAAAAGGTAGTGCACCTGTAACTGCTGGCGGCAAGCCCGGTGGTGTCGTACACGCTGGTGGTTTAGCACCTACTGCCGGCGGCAGCGTCCCAGCTCCTGGAGGTGGCGGATCCATCACATACCACGGAGGGCCTCTCATGACCGGCGACATTAACCTTTCCATCCTATTTTACGGCCAGTTCACAGCCGAACAAAAGAACGTTATCCGGAGTTTCCTGAGATCCCTCGAGAACACGGAGAACGATCACGTCGCCGCCGTGCACAGGTGGTGGGACGTCATCGAGAGCTACCAATTGTTCGCTGGTCCAAACCCCGGTAAAGGTACTCCACCCAGACTCCGGATCAAGGTGGGGGCCCAGCAGAGCGACGACAAGTACTCGATCGGAAAAGTGATAACCTTTGAGTTCATCAAGAGACTGGTGAATAAGGCCGGCTCCGGAAAGCCCAACACTATTAACGTGATCTTCACAGGGAGCCAAGTGACGGTGCACAGCTTGTGCCGTGGCAAATGCTATGAGCACGGCTTGATTGAGAACAGACCCTACCTCGTCGTCGGAAACCCTGAAATCGAGTGTCCCGGGGCTTGCGGTTGGCCATTCCAGAGGCTGGACTACGGTACTCCTAATCAAGTGACGGTGAAGCCCCCCAACAGCAACATGGGCATTGACGCCATGCTCTTAAACTTCGCCTCCGGCTTGGCTGCCACCGTCACGAACCCATTCAACACTGGATTCTTCAAGCCGGGACGAAAGAGCGACCCCGTCGAGGCCGCCACCGCTTGCGAAAACATCTTCGGAAGCGGAGCTGTCCCAGGGAAGACTGGCAAGGTTGAGGTCGACCGCGCTACTGGTGGGTCTTACAATGCCGTGGGAGAGAAGGGCATGAAGTTCATGCTCCCCGCCGTCTGGAACCCACGAACTTCCTCCTGCTGGACGGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

404

Amino Acids

41.7

Weight (kDa)

9.9

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 128 - 401 2.2e-89 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 546
AccB7I CCANNNNNTGG 2 cut(s) 365, 874
AccI GTMKAC 1 cut(s) 1104
AccII CGCG 2 cut(s) 141, 1110
AccIII TCCGGA 3 cut(s) 474, 606, 704
AclI AACGTT 1 cut(s) 468
AclWI GGATC 4 cut(s) 372, 385, 483, 617
AcoI YGGCCR 3 cut(s) 442, 860, 1203
AcyI GRCGYC 2 cut(s) 543, 936
AfaI GTAC 4 cut(s) 318, 592, 644, 886
AgsI TTSAA 2 cut(s) 991, 1006
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 3 cut(s) 39, 364, 1078
AleI CACNNNNGTG 4 cut(s) 186, 204, 240, 324
AluBI AGCT 5 cut(s) 62, 362, 555, 762, 1073
AluI AGCT 5 cut(s) 62, 362, 555, 762, 1073
Alw21I GWGCWC 9 cut(s) 181, 199, 217, 235, 253, 283, 531, 759, 789
Alw26I GTCTC 1 cut(s) 676
Alw44I GTGCAC 8 cut(s) 177, 195, 213, 231, 249, 279, 527, 755
AlwI GGATC 4 cut(s) 372, 385, 483, 617
AlwNI CAGNNNCTG 2 cut(s) 365, 874
Ama87I CYCGRG 2 cut(s) 494, 845
Aor13HI TCCGGA 3 cut(s) 474, 606, 704
AoxI GGCC 8 cut(s) 136, 398, 442, 621, 696, 860, 1035, 1203
ApaI GGGCCC 1 cut(s) 625
ApaLI GTGCAC 8 cut(s) 177, 195, 213, 231, 249, 279, 527, 755
ApeKI GCWGC 4 cut(s) 5, 59, 351, 968
Asp700I GAANNNNTTC 1 cut(s) 986
AspLEI GCGC 1 cut(s) 1112
AspS9I GGNCC 4 cut(s) 398, 572, 621, 622
AsuC2I CCSGG 5 cut(s) 306, 582, 846, 847, 1011
AsuHPI GGTGA 2 cut(s) 700, 916
AvaI CYCGRG 2 cut(s) 494, 845
AvaII GGWCC 1 cut(s) 572
BaeGI GKGCMC 9 cut(s) 181, 199, 217, 235, 253, 283, 531, 625, 759
BalI TGGCCA 1 cut(s) 862
BamHI GGATCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 1091
Bbv12I GWGCWC 9 cut(s) 181, 199, 217, 235, 253, 283, 531, 759, 789
BbvI GCAGC 4 cut(s) 17, 71, 363, 955
BccI CCATC 2 cut(s) 389, 437
BceAI ACGGC 6 cut(s) 457, 509, 753, 805, 1118, 1154
BciT130I CCWGG 3 cut(s) 41, 366, 1080
BcnI CCSGG 5 cut(s) 306, 582, 846, 847, 1011
BcoDI GTCTC 1 cut(s) 676
BmcAI AGTACT 1 cut(s) 644
Bme1390I CCNGG 8 cut(s) 41, 306, 366, 582, 846, 847, 1011, 1080
Bme18I GGWCC 1 cut(s) 572
BmeT110I CYCGRG 2 cut(s) 494, 845
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 4 cut(s) 398, 572, 621, 622
BmiI GGNNCC 7 cut(s) 47, 379, 622, 623, 703, 743, 1178
BmrFI CCNGG 8 cut(s) 41, 306, 366, 582, 846, 847, 1011, 1080
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 1091
BplI GAGNNNNNCTC 2 cut(s) 387, 419
BpmI CTGGAG 1 cut(s) 387
BpuMI CCSGG 5 cut(s) 306, 582, 846, 847, 1011
Bsa29I ATCGAT 1 cut(s) 90
BsaHI GRCGYC 2 cut(s) 543, 936
BsaWI WCCGGW 3 cut(s) 474, 606, 704
BsaXI ACNNNNNCTCC 4 cut(s) 588, 618, 941, 971
Bse118I RCCGGY 4 cut(s) 48, 344, 410, 698
Bse1I ACTGG 6 cut(s) 23, 445, 690, 1000, 1093, 1120
BseAI TCCGGA 3 cut(s) 474, 606, 704
BseBI CCWGG 3 cut(s) 41, 366, 1080
BseCI ATCGAT 1 cut(s) 90
BseGI GGATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 6 cut(s) 23, 445, 690, 1000, 1093, 1120
BseSI GKGCMC 9 cut(s) 181, 199, 217, 235, 253, 283, 531, 625, 759
BseXI GCAGC 4 cut(s) 17, 71, 363, 955
BseYI CCCAGC 2 cut(s) 358, 624
Bsh1236I CGCG 2 cut(s) 141, 1110
Bsh1285I CGRYCG 3 cut(s) 268, 651, 1108
BshFI GGCC 8 cut(s) 138, 400, 444, 623, 698, 862, 1037, 1205
BshVI ATCGAT 1 cut(s) 90
BsiEI CGRYCG 3 cut(s) 268, 651, 1108
BsiHKAI GWGCWC 9 cut(s) 181, 199, 217, 235, 253, 283, 531, 759, 789
BsiHKCI CYCGRG 2 cut(s) 494, 845
BslFI GGGAC 5 cut(s) 341, 554, 828, 1026, 1061
BsmAI GTCTC 1 cut(s) 676
BsmFI GGGAC 5 cut(s) 341, 554, 828, 1026, 1061
BsnI GGCC 8 cut(s) 138, 400, 444, 623, 698, 862, 1037, 1205
BsoBI CYCGRG 2 cut(s) 494, 845
Bsp120I GGGCCC 1 cut(s) 621
Bsp13I TCCGGA 3 cut(s) 474, 606, 704
Bsp143I GATC 6 cut(s) 377, 488, 511, 609, 648, 729
BspANI GGCC 8 cut(s) 138, 400, 444, 623, 698, 862, 1037, 1205
BspCNI CTCAG 1 cut(s) 477
BspDI ATCGAT 1 cut(s) 90
BspEI TCCGGA 3 cut(s) 474, 606, 704
BspFNI CGCG 2 cut(s) 141, 1110
BspHI TCATGA 1 cut(s) 405
BspLI GGNNCC 7 cut(s) 47, 379, 622, 623, 703, 743, 1178
BspPI GGATC 4 cut(s) 372, 385, 483, 617
BsrFI RCCGGY 4 cut(s) 48, 344, 410, 698
BsrI ACTGG 6 cut(s) 23, 445, 690, 1000, 1093, 1120
BssAI RCCGGY 4 cut(s) 48, 344, 410, 698
BssMI GATC 6 cut(s) 377, 488, 511, 609, 648, 729
BssNI GRCGYC 2 cut(s) 543, 936
Bst2UI CCWGG 3 cut(s) 41, 366, 1080
Bst4CI ACNGT 4 cut(s) 754, 884, 904, 976
Bst6I CTCTTC 1 cut(s) 127
BstACI GRCGYC 2 cut(s) 543, 936
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 7 cut(s) 57, 295, 302, 346, 700, 853, 1048
BstDEI CTNAG 1 cut(s) 485
BstDSI CCRYGG 4 cut(s) 139, 391, 769, 1134
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 2 cut(s) 141, 1110
BstHHI GCGC 1 cut(s) 1112
BstKTI GATC 6 cut(s) 380, 491, 514, 612, 651, 732
BstMAI GTCTC 1 cut(s) 676
BstMBI GATC 6 cut(s) 377, 488, 511, 609, 648, 729
BstMCI CGRYCG 3 cut(s) 268, 651, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 65, 341, 927, 1043
BstNI CCWGG 3 cut(s) 41, 366, 1080
BstSCI CCNGG 8 cut(s) 39, 304, 364, 580, 844, 845, 1009, 1078
BstSLI GKGCMC 9 cut(s) 181, 199, 217, 235, 253, 283, 531, 625, 759
BstUI CGCG 2 cut(s) 141, 1110
BstV1I GCAGC 4 cut(s) 17, 71, 363, 955
BstV2I GAAGAC 1 cut(s) 1091
BstX2I RGATCY 2 cut(s) 377, 488
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 2 cut(s) 377, 488
Bsu15I ATCGAT 1 cut(s) 90
BsuRI GGCC 8 cut(s) 138, 400, 444, 623, 698, 862, 1037, 1205
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 4 cut(s) 139, 391, 769, 1134
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 30, 993
Cac8I GCNNGC 7 cut(s) 57, 295, 302, 346, 700, 853, 1048
CaiI CAGNNNCTG 2 cut(s) 365, 874
CciI TCATGA 1 cut(s) 405
CfoI GCGC 1 cut(s) 1112
Cfr10I RCCGGY 4 cut(s) 48, 344, 410, 698
Cfr13I GGNCC 4 cut(s) 398, 572, 621, 622
Cfr42I CCGCGG 1 cut(s) 142
Cfr9I CCCGGG 1 cut(s) 845
ClaI ATCGAT 1 cut(s) 90
CseI GACGC 2 cut(s) 343, 944
Csp6I GTAC 4 cut(s) 317, 591, 643, 885
CspCI CAANNNNNGTGG 4 cut(s) 190, 225, 289, 324
CviAII CATG 6 cut(s) 406, 925, 940, 1150, 1159, 1207
CviQI GTAC 4 cut(s) 317, 591, 643, 885
DdeI CTNAG 1 cut(s) 485
DpnI GATC 6 cut(s) 379, 490, 513, 611, 650, 731
DpnII GATC 6 cut(s) 377, 488, 511, 609, 648, 729
EaeI YGGCCR 3 cut(s) 442, 860, 1203
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
EciI GGCGGA 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 572
Eco88I CYCGRG 2 cut(s) 494, 845
EcoO109I RGGNCCY 2 cut(s) 398, 621
EcoRII CCWGG 3 cut(s) 39, 364, 1078
EcoT38I GRGCYC 1 cut(s) 625
FaeI CATG 6 cut(s) 409, 928, 943, 1153, 1162, 1210
FaiI YATR 8 cut(s) 388, 407, 783, 926, 941, 1151, 1160, 1208
FaqI GGGAC 5 cut(s) 341, 554, 828, 1026, 1061
FatI CATG 6 cut(s) 405, 924, 939, 1149, 1158, 1206
FauI CCCGC 1 cut(s) 1174
FblI GTMKAC 1 cut(s) 1104
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 1 cut(s) 625
GlaI GCGC 1 cut(s) 1111
GsaI CCCAGC 2 cut(s) 362, 628
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 8 cut(s) 138, 400, 444, 623, 698, 862, 1037, 1205
HgaI GACGC 2 cut(s) 343, 944
HhaI GCGC 1 cut(s) 1112
Hin1I GRCGYC 2 cut(s) 543, 936
Hin1II CATG 6 cut(s) 409, 928, 943, 1153, 1162, 1210
Hin6I GCGC 1 cut(s) 1110
HinP1I GCGC 1 cut(s) 1110
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HinfI GANTC 2 cut(s) 603, 999
HphI GGTGA 2 cut(s) 700, 916
Hpy188I TCNGA 3 cut(s) 653, 824, 1064
Hpy99I CGWCG 5 cut(s) 521, 638, 821, 824, 1034
HpyAV CCTTC 1 cut(s) 1138
HpyCH4III ACNGT 4 cut(s) 754, 884, 904, 976
HpyCH4IV ACGT 4 cut(s) 468, 516, 543, 726
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 341, 927, 1043
HpyF3I CTNAG 1 cut(s) 485
HpySE526I ACGT 4 cut(s) 468, 516, 543, 726
Hsp92I GRCGYC 2 cut(s) 543, 936
Hsp92II CATG 6 cut(s) 409, 928, 943, 1153, 1162, 1210
HspAI GCGC 1 cut(s) 1110
Kpn2I TCCGGA 3 cut(s) 474, 606, 704
KroI GCCGGC 2 cut(s) 344, 698
KroNI GCCGGC 2 cut(s) 346, 700
KspI CCGCGG 1 cut(s) 142
Kzo9I GATC 6 cut(s) 377, 488, 511, 609, 648, 729
LmnI GCTCC 5 cut(s) 367, 707, 741, 1070, 1167
Lsp1109I GCAGC 4 cut(s) 17, 71, 363, 955
LweI GCATC 1 cut(s) 17
MaeII ACGT 4 cut(s) 468, 516, 543, 726
MaeIII GTNAC 4 cut(s) 286, 748, 898, 976
MalI GATC 6 cut(s) 379, 490, 513, 611, 650, 731
MboI GATC 6 cut(s) 377, 488, 511, 609, 648, 729
MboII GAAGA 6 cut(s) 6, 144, 724, 994, 1051, 1096
MfeI CAATTG 1 cut(s) 560
MflI RGATCY 2 cut(s) 377, 488
MlsI TGGCCA 1 cut(s) 862
MluCI AATT 1 cut(s) 560
MluNI TGGCCA 1 cut(s) 862
MlyI GAGTC 1 cut(s) 597
MmeI TCCRAC 2 cut(s) 147, 802
Mox20I TGGCCA 1 cut(s) 862
MroI TCCGGA 3 cut(s) 474, 606, 704
MroNI GCCGGC 2 cut(s) 344, 698
MroXI GAANNNNTTC 1 cut(s) 986
MscI TGGCCA 1 cut(s) 862
MseI TTAA 3 cut(s) 420, 723, 947
MslI CAYNNNNRTG 4 cut(s) 186, 204, 240, 324
Msp20I TGGCCA 1 cut(s) 862
MspA1I CMGCKG 1 cut(s) 141
MspR9I CCNGG 8 cut(s) 41, 306, 366, 582, 846, 847, 1011, 1080
MunI CAATTG 1 cut(s) 560
MvaI CCWGG 3 cut(s) 41, 366, 1080
MvnI CGCG 2 cut(s) 141, 1110
MwoI GCNNNNNNNGC 4 cut(s) 65, 341, 927, 1043
NaeI GCCGGC 2 cut(s) 346, 700
NciI CCSGG 5 cut(s) 306, 582, 846, 847, 1011
NdeII GATC 6 cut(s) 377, 488, 511, 609, 648, 729
NgoMIV GCCGGC 2 cut(s) 344, 698
NlaIII CATG 6 cut(s) 409, 928, 943, 1153, 1162, 1210
NlaIV GGNNCC 7 cut(s) 47, 379, 622, 623, 703, 743, 1178
NmuCI GTSAC 3 cut(s) 748, 898, 976
OliI CACNNNNGTG 4 cut(s) 186, 204, 240, 324
PaeR7I CTCGAG 1 cut(s) 494
PagI TCATGA 1 cut(s) 405
PasI CCCWGGG 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PdiI GCCGGC 2 cut(s) 346, 700
PdmI GAANNNNTTC 1 cut(s) 986
PfeI GAWTC 1 cut(s) 999
PflFI GACNNNGTC 1 cut(s) 1028
PflMI CCANNNNNTGG 2 cut(s) 365, 874
PfoI TCCNGGA 1 cut(s) 364
Ple19I CGATCG 1 cut(s) 651
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
Psp1406I AACGTT 1 cut(s) 468
Psp6I CCWGG 3 cut(s) 39, 364, 1078
PspFI CCCAGC 2 cut(s) 358, 624
PspGI CCWGG 3 cut(s) 39, 364, 1078
PspN4I GGNNCC 7 cut(s) 47, 379, 622, 623, 703, 743, 1178
PspOMI GGGCCC 1 cut(s) 621
PspPI GGNCC 4 cut(s) 398, 572, 621, 622
PsrI GAACNNNNNNTAC 2 cut(s) 794, 826
PstNI CAGNNNCTG 2 cut(s) 365, 874
PsuI RGATCY 2 cut(s) 377, 488
PsyI GACNNNGTC 1 cut(s) 1028
PvuI CGATCG 1 cut(s) 651
RsaI GTAC 4 cut(s) 318, 592, 644, 886
RsaNI GTAC 4 cut(s) 317, 591, 643, 885
RseI CAYNNNNRTG 4 cut(s) 186, 204, 240, 324
SacII CCGCGG 1 cut(s) 142
SalI GTCGAC 1 cut(s) 1103
SaqAI TTAA 3 cut(s) 420, 723, 947
Sau3AI GATC 6 cut(s) 377, 488, 511, 609, 648, 729
Sau96I GGNCC 4 cut(s) 398, 572, 621, 622
ScaI AGTACT 1 cut(s) 644
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 8 cut(s) 41, 306, 366, 582, 846, 847, 1011, 1080
SfaNI GCATC 1 cut(s) 17
Sfr274I CTCGAG 1 cut(s) 494
Sfr303I CCGCGG 1 cut(s) 142
SgrBI CCGCGG 1 cut(s) 142
SinI GGWCC 1 cut(s) 572
SlaI CTCGAG 1 cut(s) 494
SmaI CCCGGG 1 cut(s) 847
SmiMI CAYNNNNRTG 4 cut(s) 186, 204, 240, 324
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 1 cut(s) 560
StyD4I CCNGG 8 cut(s) 39, 304, 364, 580, 844, 845, 1009, 1078
TaaI ACNGT 4 cut(s) 754, 884, 904, 976
TaiI ACGT 4 cut(s) 471, 519, 546, 729
TaqI TCGA 8 cut(s) 90, 268, 495, 549, 647, 837, 1032, 1104
TasI AATT 1 cut(s) 560
TatI WGTACW 1 cut(s) 642
TauI GCSGC 6 cut(s) 55, 141, 300, 351, 524, 1040
TfiI GAWTC 1 cut(s) 999
Tru1I TTAA 3 cut(s) 420, 723, 947
Tru9I TTAA 3 cut(s) 420, 723, 947
TscAI CASTG 2 cut(s) 30, 1000
TseFI GTSAC 3 cut(s) 748, 898, 976
TseI GCWGC 4 cut(s) 5, 59, 351, 968
Tsp45I GTSAC 3 cut(s) 748, 898, 976
TspDTI ATGAA 3 cut(s) 664, 1147, 1166
TspGWI ACGGA 2 cut(s) 408, 518
TspMI CCCGGG 1 cut(s) 845
TspRI CASTG 2 cut(s) 30, 1000
Tth111I GACNNNGTC 1 cut(s) 1028
Van91I CCANNNNNTGG 2 cut(s) 365, 874
VneI GTGCAC 8 cut(s) 177, 195, 213, 231, 249, 279, 527, 755
VpaK11BI GGWCC 1 cut(s) 572
XcmI CCANNNNNNNNNTGG 2 cut(s) 566, 922
XhoI CTCGAG 1 cut(s) 494
XmaI CCCGGG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 1 cut(s) 986
ZraI GACGTC 1 cut(s) 544
ZrmI AGTACT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.