Rorug01G0447300

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005922
Physical Location & Seq
Forward (+)
54280055 .. 54283513
3459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0447300.1

Sequence Viewer

Length: 936 bp
ATGGCACCTCCTCGAGGCCGTGGTGGTTCTGGTGGGTTCAGGGGTGGAAGGAGTGATGGAGGGAGAGGCCGAGGTGGAGGCCGTGGATTCGGTGACAGAAGTAACAGTGGTTTTAAGCCCAGTGGAGGCCGAGGTGACCGTGGTGGTCGGGGCCGTGGTGACCGCGGCCGTGGAAGAGGCCGTGGGGGCAGAGGAGGAATGAAGGGTGGAACCAAGGTTGTGGTTGAGCCCCATAGACATGCTGGGGTTTTCCTTGCTAAGGGTAAAGAAGATGCTCTTGTGACCAAGAACATGGTCCCCGGAGAAGCTGTCTACAATGAGAAGAGGATTTCCGTTCAGCAAGAGGATGGAACAAAAGTTGAGTATAGGATTTGGAACCCTTTCCGTTCCAAGTTGGCAGCTGCTATTCTTGGTGGTGTTGATGAGATCTGGATTAAACCTGGTGCCAAGGTTCTCTACCTGGGGGCTGCTTCTGGTACCACAGTCTCTCATGTCTCCGACCTTGTTGGCCCTACTGGATGTGTCTATGCAGTGGAGTTTTCTCATAGAAGTGGCAGAGACTTGGTAAACATGGCTAAGAAGAGAACTAATGTCATACCAATTATTGAAGATGCTAGGCATCCAGCGAAATACCGTATGCTTGTTGGCATGGTTGACGTGATATTTTCTGATGTTGCACAACCCGATCAGGCCAGGATTCTAGCTTTGAATGCATCATATTTTCTTAAAGCAGGAGGGCACTTTGTCATCTCTATCAAGGCTAATTGTATCGACTGCACTGTTCCTGCTGAGGTGGTGTTCCAATCTGAGGTGAAGAAGCTGCAGGCAGAACAGTTTAAGCCTTTTGAACAAGTGACTCTTGAACCTTTTGAGCGTGATCACGCTTGTGTGATTGGTGGTTACCGCATGCCAAAGAAGTCAAAGGCTGCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

311

Amino Acids

33.29

Weight (kDa)

10.09

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fibrillarin PF01269 75 - 302 1.2e-108 Fibrillarin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 12
Acc65I GGTACC 1 cut(s) 476
AccB1I GGYRCC 3 cut(s) 4, 443, 476
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 165
AciI CCGC 3 cut(s) 163, 165, 904
AcoI YGGCCR 1 cut(s) 166
AfaI GTAC 1 cut(s) 478
AfiI CCNNNNNNNGG 4 cut(s) 14, 125, 259, 808
AgsI TTSAA 4 cut(s) 608, 709, 848, 863
AjiI CACGTC 1 cut(s) 658
AjnI CCWGG 3 cut(s) 439, 459, 692
AleI CACNNNNGTG 1 cut(s) 885
AluBI AGCT 4 cut(s) 308, 401, 704, 820
AluI AGCT 4 cut(s) 308, 401, 704, 820
Alw26I GTCTC 3 cut(s) 490, 499, 552
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 9 cut(s) 16, 67, 79, 127, 151, 166, 178, 508, 690
ApeKI GCWGC 6 cut(s) 398, 401, 467, 820, 926, 929
Asp700I GAANNNNTTC 1 cut(s) 380
Asp718I GGTACC 1 cut(s) 476
AspS9I GGNCC 3 cut(s) 151, 295, 509
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 4 cut(s) 104, 146, 170, 823
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 741
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BanI GGYRCC 3 cut(s) 4, 443, 476
BanII GRGCYC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 789
BbvI GCAGC 6 cut(s) 388, 410, 454, 807, 913, 916
BccI CCATC 2 cut(s) 50, 341
BceAI ACGGC 5 cut(s) 3, 66, 138, 153, 165
BciT130I CCWGG 3 cut(s) 441, 461, 694
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 3 cut(s) 490, 499, 552
BfaI CTAG 3 cut(s) 615, 701, 934
BfmI CTRYAG 1 cut(s) 821
BglI GCCNNNNNGGC 1 cut(s) 186
BglII AGATCT 1 cut(s) 426
BisI GCNGC 7 cut(s) 166, 399, 402, 468, 821, 927, 930
BlsI GCNGC 7 cut(s) 167, 400, 403, 469, 822, 928, 931
Bme1390I CCNGG 4 cut(s) 300, 441, 461, 694
Bme18I GGWCC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 658
BmgT120I GGNCC 3 cut(s) 151, 295, 509
BmiI GGNNCC 7 cut(s) 6, 152, 211, 297, 377, 445, 478
BmrFI CCNGG 4 cut(s) 300, 441, 461, 694
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 4 cut(s) 262, 601, 628, 722
BmuI ACTGGG 1 cut(s) 114
Bpu10I CCTNAGC 2 cut(s) 258, 789
BpuMI CCSGG 1 cut(s) 300
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 125, 259, 808
Bse1I ACTGG 2 cut(s) 120, 520
BseBI CCWGG 3 cut(s) 441, 461, 694
BseGI GGATG 3 cut(s) 352, 524, 619
BseLI CCNNNNNNNGG 4 cut(s) 14, 125, 259, 808
BseMII CTCAG 2 cut(s) 780, 798
BseNI ACTGG 2 cut(s) 120, 520
BseRI GAGGAG 1 cut(s) 207
BseSI GKGCMC 1 cut(s) 741
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 6 cut(s) 388, 410, 454, 807, 913, 916
BseYI CCCAGC 1 cut(s) 242
BsgI GTGCAG 1 cut(s) 760
Bsh1236I CGCG 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 9 cut(s) 18, 69, 81, 129, 153, 168, 180, 510, 692
BshNI GGYRCC 3 cut(s) 4, 443, 476
BsiEI CGRYCG 1 cut(s) 169
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 4 cut(s) 14, 125, 259, 808
BsmAI GTCTC 3 cut(s) 490, 499, 552
BsmFI GGGAC 1 cut(s) 281
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 9 cut(s) 18, 69, 81, 129, 153, 168, 180, 510, 692
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 231, 741
Bsp143I GATC 3 cut(s) 426, 685, 877
BspACI CCGC 3 cut(s) 163, 165, 904
BspANI GGCC 9 cut(s) 18, 69, 81, 129, 153, 168, 180, 510, 692
BspCNI CTCAG 2 cut(s) 781, 799
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 7 cut(s) 6, 152, 211, 297, 377, 445, 478
BspMAI CTGCAG 1 cut(s) 825
BspT107I GGYRCC 3 cut(s) 4, 443, 476
BsrI ACTGG 2 cut(s) 120, 520
BssMI GATC 3 cut(s) 426, 685, 877
BssT1I CCWWGG 2 cut(s) 213, 447
Bst2UI CCWGG 3 cut(s) 441, 461, 694
Bst4CI ACNGT 6 cut(s) 107, 140, 484, 635, 781, 834
Bst6I CTCTTC 3 cut(s) 169, 317, 575
BstC8I GCNNGC 2 cut(s) 825, 908
BstDEI CTNAG 4 cut(s) 258, 576, 789, 807
BstDSI CCRYGG 7 cut(s) 19, 82, 139, 154, 163, 169, 181
BstEII GGTNACC 3 cut(s) 134, 158, 899
BstENI CCTNNNNNAGG 2 cut(s) 12, 257
BstF5I GGATG 3 cut(s) 352, 524, 619
BstFNI CGCG 1 cut(s) 165
BstKTI GATC 3 cut(s) 429, 688, 880
BstMAI GTCTC 3 cut(s) 490, 499, 552
BstMBI GATC 3 cut(s) 426, 685, 877
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 186, 710
BstNI CCWGG 3 cut(s) 441, 461, 694
BstNSI RCATGY 2 cut(s) 242, 910
BstPI GGTNACC 3 cut(s) 134, 158, 899
BstSCI CCNGG 4 cut(s) 298, 439, 459, 692
BstSFI CTRYAG 1 cut(s) 821
BstSLI GKGCMC 1 cut(s) 741
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 6 cut(s) 388, 410, 454, 807, 913, 916
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 2 cut(s) 220, 292
BstYI RGATCY 1 cut(s) 426
BstZI CGGCCG 1 cut(s) 166
BsuRI GGCC 9 cut(s) 18, 69, 81, 129, 153, 168, 180, 510, 692
BtgI CCRYGG 7 cut(s) 19, 82, 139, 154, 163, 169, 181
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 3 cut(s) 352, 524, 619
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 4 cut(s) 112, 127, 537, 777
Cac8I GCNNGC 2 cut(s) 825, 908
Cfr13I GGNCC 3 cut(s) 151, 295, 509
Cfr42I CCGCGG 1 cut(s) 166
CsiI ACCWGGT 1 cut(s) 439
Csp6I GTAC 1 cut(s) 477
CviAII CATG 6 cut(s) 239, 292, 491, 571, 649, 907
CviQI GTAC 1 cut(s) 477
DdeI CTNAG 4 cut(s) 258, 576, 789, 807
DpnI GATC 3 cut(s) 428, 687, 879
DpnII GATC 3 cut(s) 426, 685, 877
EaeI YGGCCR 1 cut(s) 166
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 3 cut(s) 169, 317, 575
EarI CTCTTC 3 cut(s) 169, 317, 575
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 213, 447
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 295
Eco52I CGGCCG 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 12
Eco91I GGTNACC 3 cut(s) 134, 158, 899
EcoNI CCTNNNNNAGG 2 cut(s) 12, 257
EcoO65I GGTNACC 3 cut(s) 134, 158, 899
EcoRII CCWGG 3 cut(s) 439, 459, 692
EcoT14I CCWWGG 2 cut(s) 213, 447
EcoT22I ATGCAT 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 2 cut(s) 213, 447
FaeI CATG 6 cut(s) 242, 295, 494, 574, 652, 910
FalI AAGNNNNNCTT 4 cut(s) 261, 293, 843, 875
FaqI GGGAC 1 cut(s) 281
FatI CATG 6 cut(s) 238, 291, 490, 570, 648, 906
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 7 cut(s) 166, 399, 402, 468, 821, 927, 930
FokI GGATG 3 cut(s) 359, 531, 606
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 7 cut(s) 166, 399, 402, 468, 821, 927, 930
FspBI CTAG 3 cut(s) 615, 701, 934
GluI GCNGC 7 cut(s) 166, 399, 402, 468, 821, 927, 930
GsaI CCCAGC 1 cut(s) 246
HaeIII GGCC 9 cut(s) 18, 69, 81, 129, 153, 168, 180, 510, 692
HapII CCGG 1 cut(s) 300
Hin1II CATG 6 cut(s) 242, 295, 494, 574, 652, 910
HincII GTYRAC 1 cut(s) 655
HindII GTYRAC 1 cut(s) 655
HinfI GANTC 3 cut(s) 87, 697, 856
HpaII CCGG 1 cut(s) 300
HphI GGTGA 4 cut(s) 104, 146, 170, 823
Hpy166II GTNNAC 3 cut(s) 313, 568, 655
Hpy188I TCNGA 3 cut(s) 499, 670, 808
Hpy188III TCNNGA 2 cut(s) 430, 860
Hpy8I GTNNAC 3 cut(s) 313, 568, 655
HpyAV CCTTC 2 cut(s) 42, 196
HpyCH4III ACNGT 6 cut(s) 107, 140, 484, 635, 781, 834
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 5 cut(s) 530, 677, 713, 777, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 710
HpyF3I CTNAG 4 cut(s) 258, 576, 789, 807
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 6 cut(s) 242, 295, 494, 574, 652, 910
KpnI GGTACC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 877
KspI CCGCGG 1 cut(s) 166
Kzo9I GATC 3 cut(s) 426, 685, 877
Lsp1109I GCAGC 6 cut(s) 388, 410, 454, 807, 913, 916
LweI GCATC 4 cut(s) 262, 601, 628, 722
MabI ACCWGGT 1 cut(s) 439
MaeI CTAG 3 cut(s) 615, 701, 934
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 7 cut(s) 92, 101, 134, 158, 280, 853, 899
MalI GATC 3 cut(s) 428, 687, 879
MboI GATC 3 cut(s) 426, 685, 877
MboII GAAGA 6 cut(s) 186, 281, 334, 592, 620, 826
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 2 cut(s) 231, 741
MluCI AATT 2 cut(s) 600, 763
MlyI GAGTC 1 cut(s) 850
MmeI TCCRAC 1 cut(s) 522
Mph1103I ATGCAT 1 cut(s) 715
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 4 cut(s) 114, 435, 726, 837
MslI CAYNNNNRTG 3 cut(s) 237, 549, 885
MspA1I CMGCKG 2 cut(s) 165, 401
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 4 cut(s) 300, 441, 461, 694
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 3 cut(s) 441, 461, 694
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 186, 710
NciI CCSGG 1 cut(s) 300
NdeII GATC 3 cut(s) 426, 685, 877
NlaIII CATG 6 cut(s) 242, 295, 494, 574, 652, 910
NlaIV GGNNCC 7 cut(s) 6, 152, 211, 297, 377, 445, 478
NmeAIII GCCGAG 2 cut(s) 95, 155
NmuCI GTSAC 5 cut(s) 92, 134, 158, 280, 853
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 2 cut(s) 242, 910
OliI CACNNNNGTG 1 cut(s) 885
PaeI GCATGC 1 cut(s) 910
PaeR7I CTCGAG 1 cut(s) 12
PctI GAATGC 1 cut(s) 715
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 2 cut(s) 87, 697
PkrI GCNGC 7 cut(s) 167, 400, 403, 469, 822, 928, 931
PleI GAGTC 1 cut(s) 850
PpsI GAGTC 1 cut(s) 850
Psp6I CCWGG 3 cut(s) 439, 459, 692
PspEI GGTNACC 3 cut(s) 134, 158, 899
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 3 cut(s) 439, 459, 692
PspN4I GGNNCC 7 cut(s) 6, 152, 211, 297, 377, 445, 478
PspPI GGNCC 3 cut(s) 151, 295, 509
PspXI VCTCGAGB 1 cut(s) 12
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 1 cut(s) 478
RsaNI GTAC 1 cut(s) 477
RseI CAYNNNNRTG 3 cut(s) 237, 549, 885
SacII CCGCGG 1 cut(s) 166
SaqAI TTAA 4 cut(s) 114, 435, 726, 837
SatI GCNGC 7 cut(s) 166, 399, 402, 468, 821, 927, 930
Sau3AI GATC 3 cut(s) 426, 685, 877
Sau96I GGNCC 3 cut(s) 151, 295, 509
SchI GAGTC 1 cut(s) 850
ScrFI CCNGG 4 cut(s) 300, 441, 461, 694
SduI GDGCHC 2 cut(s) 231, 741
SexAI ACCWGGT 1 cut(s) 439
SfaNI GCATC 4 cut(s) 262, 601, 628, 722
SfcI CTRYAG 1 cut(s) 821
Sfr274I CTCGAG 1 cut(s) 12
Sfr303I CCGCGG 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 166
SinI GGWCC 1 cut(s) 295
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 3 cut(s) 237, 549, 885
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SphI GCATGC 1 cut(s) 910
Sse9I AATT 2 cut(s) 600, 763
SsiI CCGC 3 cut(s) 163, 165, 904
SspMI CTAG 3 cut(s) 615, 701, 934
StyD4I CCNGG 4 cut(s) 298, 439, 459, 692
StyI CCWWGG 2 cut(s) 213, 447
TaaI ACNGT 6 cut(s) 107, 140, 484, 635, 781, 834
TaiI ACGT 1 cut(s) 660
TaqI TCGA 2 cut(s) 13, 771
TasI AATT 2 cut(s) 600, 763
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 2 cut(s) 87, 697
Tru1I TTAA 4 cut(s) 114, 435, 726, 837
Tru9I TTAA 4 cut(s) 114, 435, 726, 837
TscAI CASTG 4 cut(s) 112, 127, 537, 784
TseFI GTSAC 5 cut(s) 92, 134, 158, 280, 853
TseI GCWGC 6 cut(s) 398, 401, 467, 820, 926, 929
Tsp45I GTSAC 5 cut(s) 92, 134, 158, 280, 853
TspDTI ATGAA 1 cut(s) 215
TspGWI ACGGA 2 cut(s) 322, 374
TspRI CASTG 4 cut(s) 112, 127, 537, 784
VpaK11BI GGWCC 1 cut(s) 295
XagI CCTNNNNNAGG 2 cut(s) 12, 257
XceI RCATGY 2 cut(s) 242, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XhoI CTCGAG 1 cut(s) 12
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 3 cut(s) 615, 701, 934
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.