pycom03g08340

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005922
Physical Location & Seq
Forward (+)
6734289 .. 6735446
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08340.1

Sequence Viewer

Length: 1158 bp
ATGGCAGCATCATCTTCCCTCCCAATGATGGGTAGTCGCTTGGGGAACCGGCGGCTTGCAGCTCTCGCTCTCTGCCTTGTATTGCTCCTCCATGTTACTACCACTACTTTGGTATTGTCCTCCAACAAGGGAAAAGGCGGCGGTGCAAAAAAAGGTGGCACACAAAAAGGTGGTGGTGCACAGAAACGTGGCGGTGCTCAGAAAGGTGGCGATCGAAAAGGTGGTGCACCTGGAACTGCTGGCGGTAAACCCCGTGGTATCGTACACGTTGGTGATGCAGCACCTGCTGCCGGCGGCAGCTTCCGAGCTCGTGGAAGTGGCGGAGCCATCACATACCACGGAGGGCCTCTGATGACCGGCGAAATTAACCTTTCCATCCTATTTTACGGCCAGTTTACAGCCGAACAAAAGAACGTGATTCAGAGTTTCCTGAGGTCCCTCGAGAACACGGAGAACGATCACGTCGCCGCCGTGCAAAGGTGGTGGGACGTCATCGAGAGCTACCAATTGTTCACTGGTCCAACCCCCGCTACCGGTACCCCACCTAGACTCCGCATCAAGGTGGGCATCCAACAGAGCGACGACAAGTACTCGATCGGAAAAGTGTTAACCCTTACATACTTCAAGAATCTGGTGAAGAAGGCCGACTCCGGCAAGCCCAACACTCTTTTCGTGTTCTTCACCGGGAGCCAAGTGACGGTGCACCAATTGTGCCGTGGCAAATGCTATGACCACGGCTTAATTGACAACAAACCATACCTCGTCGTCGGAAACCCCGAAATCTTGTGCCGCGGGGCTTGCGGTTGGCCATTCCAGAGGCTGGACTACGGTACGCCTAATCAAGTGACGGTGAAGCCCCCCAACGGCAACATGGGCATTGACGCCATGCTCGCCCACTTCGCCTCCGGCTTGGCAGCCGTCGTCACCAACCCATTCAACACTGGATTCTTCAAGCCGGGACCCAAGAGCGACCCCATCGAGGCCGGCACCGCCTGCGACAACATCTTCGGAAGCGGTGCTGTCCCAGGCAAGACCGGCAAGGTATTGGTCGACCCCGCTACCGGTGGGTCCTATAATGCTGTCGGAGAGAAGGGCATGAAGTTCATGCTCCCCGCCGTCTGGAACCCACGAACTTCCTCCTGCTGGACGGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

386

Amino Acids

40.37

Weight (kDa)

9.77

Isoelectric Point (pI)

27.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 110 - 383 1.3e-85 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 292
AatII GACGTC 1 cut(s) 492
Acc36I ACCTGC 1 cut(s) 292
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 2 cut(s) 536, 986
AccB7I CCANNNNNTGG 1 cut(s) 820
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 1 cut(s) 792
AcoI YGGCCR 3 cut(s) 388, 806, 1149
AcyI GRCGYC 2 cut(s) 489, 882
AfaI GTAC 4 cut(s) 264, 538, 590, 832
AflIII ACRYGT 1 cut(s) 265
AgeI ACCGGT 2 cut(s) 533, 1061
AgsI TTSAA 3 cut(s) 625, 937, 952
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 2 cut(s) 229, 1024
AjuI GAANNNNNNNTTGG 2 cut(s) 653, 685
AleI CACNNNNGTG 1 cut(s) 270
AluBI AGCT 4 cut(s) 62, 300, 308, 501
AluI AGCT 4 cut(s) 62, 300, 308, 501
Alw21I GWGCWC 5 cut(s) 181, 199, 229, 310, 705
Alw44I GTGCAC 3 cut(s) 177, 225, 701
AlwNI CAGNNNCTG 2 cut(s) 284, 820
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 6 cut(s) 344, 388, 642, 806, 981, 1149
ApaLI GTGCAC 3 cut(s) 177, 225, 701
ApeKI GCWGC 6 cut(s) 5, 59, 278, 287, 297, 914
ArsI GACNNNNNNTTYG 2 cut(s) 989, 1021
AsiGI ACCGGT 2 cut(s) 533, 1061
Asp718I GGTACC 1 cut(s) 536
AspS9I GGNCC 5 cut(s) 344, 435, 518, 959, 1068
AsuC2I CCSGG 2 cut(s) 685, 957
AsuHPI GGTGA 5 cut(s) 284, 646, 673, 862, 916
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 4 cut(s) 435, 518, 959, 1068
AxyI CCTNAGG 1 cut(s) 431
BaeGI GKGCMC 3 cut(s) 181, 229, 705
BalI TGGCCA 1 cut(s) 808
BanI GGYRCC 2 cut(s) 536, 986
BanII GRGCYC 1 cut(s) 310
BauI CACGAG 1 cut(s) 309
Bbv12I GWGCWC 5 cut(s) 181, 199, 229, 310, 705
BbvI GCAGC 6 cut(s) 17, 71, 274, 290, 309, 926
BccI CCATC 4 cut(s) 22, 335, 383, 983
BceAI ACGGC 7 cut(s) 403, 455, 699, 751, 880, 902, 1100
BciT130I CCWGG 2 cut(s) 231, 1026
BcnI CCSGG 2 cut(s) 685, 957
BfaI CTAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 292
BmcAI AGTACT 1 cut(s) 590
Bme1390I CCNGG 4 cut(s) 231, 685, 957, 1026
Bme18I GGWCC 4 cut(s) 435, 518, 959, 1068
BmeT110I CYCGRG 1 cut(s) 440
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 5 cut(s) 344, 435, 518, 959, 1068
BmrFI CCNGG 4 cut(s) 231, 685, 957, 1026
BmsI GCATC 4 cut(s) 17, 265, 564, 576
BpuMI CCSGG 2 cut(s) 685, 957
BsaHI GRCGYC 2 cut(s) 489, 882
BsaJI CCNNGG 6 cut(s) 253, 337, 715, 733, 790, 1024
BsaWI WCCGGW 2 cut(s) 533, 1061
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 887, 917
Bse118I RCCGGY 7 cut(s) 48, 290, 356, 533, 983, 1034, 1061
Bse1I ACTGG 3 cut(s) 391, 520, 946
Bse21I CCTNAGG 1 cut(s) 431
BseBI CCWGG 2 cut(s) 231, 1026
BseDI CCNNGG 6 cut(s) 253, 337, 715, 733, 790, 1024
BseGI GGATG 2 cut(s) 375, 567
BseMII CTCAG 2 cut(s) 212, 422
BseNI ACTGG 3 cut(s) 391, 520, 946
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 3 cut(s) 181, 229, 705
BseXI GCAGC 6 cut(s) 17, 71, 274, 290, 309, 926
Bsh1236I CGCG 1 cut(s) 792
Bsh1285I CGRYCG 2 cut(s) 214, 597
BshFI GGCC 6 cut(s) 346, 390, 644, 808, 983, 1151
BshNI GGYRCC 2 cut(s) 536, 986
BshTI ACCGGT 2 cut(s) 533, 1061
BsiEI CGRYCG 2 cut(s) 214, 597
BsiHKAI GWGCWC 5 cut(s) 181, 199, 229, 310, 705
BsiHKCI CYCGRG 1 cut(s) 440
BslFI GGGAC 4 cut(s) 421, 500, 972, 1007
BsmFI GGGAC 4 cut(s) 421, 500, 972, 1007
BsnI GGCC 6 cut(s) 346, 390, 644, 808, 983, 1151
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 5 cut(s) 181, 199, 229, 310, 705
Bsp143I GATC 3 cut(s) 211, 457, 594
BspANI GGCC 6 cut(s) 346, 390, 644, 808, 983, 1151
BspCNI CTCAG 2 cut(s) 211, 423
BspFNI CGCG 1 cut(s) 792
BspMI ACCTGC 1 cut(s) 292
BspT107I GGYRCC 2 cut(s) 536, 986
BsrFI RCCGGY 7 cut(s) 48, 290, 356, 533, 983, 1034, 1061
BsrI ACTGG 3 cut(s) 391, 520, 946
BssAI RCCGGY 7 cut(s) 48, 290, 356, 533, 983, 1034, 1061
BssECI CCNNGG 6 cut(s) 253, 337, 715, 733, 790, 1024
BssMI GATC 3 cut(s) 211, 457, 594
BssNI GRCGYC 2 cut(s) 489, 882
BssSI CACGAG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 231, 1026
Bst4CI ACNGT 3 cut(s) 700, 830, 850
BstACI GRCGYC 2 cut(s) 489, 882
BstAPI GCANNNNNTGC 3 cut(s) 284, 287, 993
BstC8I GCNNGC 8 cut(s) 57, 241, 292, 656, 799, 891, 985, 994
BstDEI CTNAG 2 cut(s) 198, 431
BstDSI CCRYGG 5 cut(s) 253, 337, 715, 733, 790
BstF5I GGATG 2 cut(s) 375, 567
BstFNI CGCG 1 cut(s) 792
BstKTI GATC 3 cut(s) 214, 460, 597
BstMBI GATC 3 cut(s) 211, 457, 594
BstMCI CGRYCG 2 cut(s) 214, 597
BstNI CCWGG 2 cut(s) 231, 1026
BstSCI CCNGG 4 cut(s) 229, 683, 955, 1024
BstSLI GKGCMC 3 cut(s) 181, 229, 705
BstUI CGCG 1 cut(s) 792
BstV1I GCAGC 6 cut(s) 17, 71, 274, 290, 309, 926
BstXI CCANNNNNNTGG 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 431
BsuRI GGCC 6 cut(s) 346, 390, 644, 808, 983, 1151
BtgI CCRYGG 5 cut(s) 253, 337, 715, 733, 790
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 375, 567
BtsIMutI CAGTG 2 cut(s) 513, 939
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 8 cut(s) 57, 241, 292, 656, 799, 891, 985, 994
CaiI CAGNNNCTG 2 cut(s) 284, 820
Cfr10I RCCGGY 7 cut(s) 48, 290, 356, 533, 983, 1034, 1061
Cfr13I GGNCC 5 cut(s) 344, 435, 518, 959, 1068
Cfr42I CCGCGG 1 cut(s) 793
CseI GACGC 1 cut(s) 890
Csp6I GTAC 4 cut(s) 263, 537, 589, 831
CspAI ACCGGT 2 cut(s) 533, 1061
CspCI CAANNNNNGTGG 2 cut(s) 464, 499
CviAII CATG 6 cut(s) 92, 871, 886, 1096, 1105, 1153
CviQI GTAC 4 cut(s) 263, 537, 589, 831
DdeI CTNAG 2 cut(s) 198, 431
DpnI GATC 3 cut(s) 213, 459, 596
DpnII GATC 3 cut(s) 211, 457, 594
EaeI YGGCCR 3 cut(s) 388, 806, 1149
EciI GGCGGA 1 cut(s) 336
Ecl136II GAGCTC 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 310
Eco47I GGWCC 4 cut(s) 435, 518, 959, 1068
Eco53kI GAGCTC 1 cut(s) 308
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 308
EcoO109I RGGNCCY 4 cut(s) 344, 435, 959, 1068
EcoRII CCWGG 2 cut(s) 229, 1024
EcoT38I GRGCYC 1 cut(s) 310
FaeI CATG 6 cut(s) 95, 874, 889, 1099, 1108, 1156
FaqI GGGAC 4 cut(s) 421, 500, 972, 1007
FatI CATG 6 cut(s) 91, 870, 885, 1095, 1104, 1152
FauI CCCGC 4 cut(s) 535, 785, 1063, 1120
FblI GTMKAC 1 cut(s) 1050
FokI GGATG 2 cut(s) 362, 554
FriOI GRGCYC 1 cut(s) 310
FspBI CTAG 1 cut(s) 546
HaeIII GGCC 6 cut(s) 346, 390, 644, 808, 983, 1151
HgaI GACGC 1 cut(s) 890
Hin1I GRCGYC 2 cut(s) 489, 882
Hin1II CATG 6 cut(s) 95, 874, 889, 1099, 1108, 1156
HincII GTYRAC 2 cut(s) 609, 1051
HindII GTYRAC 2 cut(s) 609, 1051
HinfI GANTC 5 cut(s) 418, 549, 628, 647, 945
HpaI GTTAAC 1 cut(s) 609
HphI GGTGA 5 cut(s) 284, 646, 673, 862, 916
Hpy166II GTNNAC 9 cut(s) 179, 227, 248, 265, 396, 513, 609, 703, 1051
Hpy188I TCNGA 8 cut(s) 201, 305, 351, 423, 599, 770, 1010, 1085
Hpy188III TCNNGA 6 cut(s) 430, 442, 496, 625, 814, 1120
Hpy8I GTNNAC 9 cut(s) 179, 227, 248, 265, 396, 513, 609, 703, 1051
Hpy99I CGWCG 5 cut(s) 467, 584, 767, 770, 923
HpyAV CCTTC 2 cut(s) 634, 1084
HpyCH4III ACNGT 3 cut(s) 700, 830, 850
HpyCH4IV ACGT 5 cut(s) 187, 267, 414, 462, 489
HpyCH4V TGCA 7 cut(s) 59, 146, 179, 227, 278, 475, 703
HpyF3I CTNAG 2 cut(s) 198, 431
HpySE526I ACGT 5 cut(s) 187, 267, 414, 462, 489
Hsp92I GRCGYC 2 cut(s) 489, 882
Hsp92II CATG 6 cut(s) 95, 874, 889, 1099, 1108, 1156
KflI GGGWCCC 1 cut(s) 959
KpnI GGTACC 1 cut(s) 540
KroI GCCGGC 2 cut(s) 290, 983
KroNI GCCGGC 2 cut(s) 292, 985
KspAI GTTAAC 1 cut(s) 609
KspI CCGCGG 1 cut(s) 793
Kzo9I GATC 3 cut(s) 211, 457, 594
LmnI GCTCC 4 cut(s) 90, 323, 687, 1113
Lsp1109I GCAGC 6 cut(s) 17, 71, 274, 290, 309, 926
LweI GCATC 4 cut(s) 17, 265, 564, 576
MaeI CTAG 1 cut(s) 546
MaeII ACGT 5 cut(s) 187, 267, 414, 462, 489
MaeIII GTNAC 4 cut(s) 94, 694, 844, 922
MalI GATC 3 cut(s) 213, 459, 596
MboI GATC 3 cut(s) 211, 457, 594
MboII GAAGA 5 cut(s) 6, 649, 670, 940, 997
MfeI CAATTG 2 cut(s) 506, 707
MhlI GDGCHC 5 cut(s) 181, 199, 229, 310, 705
MlsI TGGCCA 1 cut(s) 808
MluCI AATT 4 cut(s) 363, 506, 707, 741
MluNI TGGCCA 1 cut(s) 808
MlyI GAGTC 2 cut(s) 543, 641
MmeI TCCRAC 5 cut(s) 147, 545, 595, 748, 1063
Mox20I TGGCCA 1 cut(s) 808
MroNI GCCGGC 2 cut(s) 290, 983
MscI TGGCCA 1 cut(s) 808
MseI TTAA 3 cut(s) 366, 608, 740
MslI CAYNNNNRTG 2 cut(s) 270, 560
Msp20I TGGCCA 1 cut(s) 808
MspA1I CMGCKG 1 cut(s) 792
MspR9I CCNGG 4 cut(s) 231, 685, 957, 1026
MunI CAATTG 2 cut(s) 506, 707
MvaI CCWGG 2 cut(s) 231, 1026
MvnI CGCG 1 cut(s) 792
NaeI GCCGGC 2 cut(s) 292, 985
NciI CCSGG 2 cut(s) 685, 957
NdeII GATC 3 cut(s) 211, 457, 594
NgoMIV GCCGGC 2 cut(s) 290, 983
NlaIII CATG 6 cut(s) 95, 874, 889, 1099, 1108, 1156
NmuCI GTSAC 3 cut(s) 694, 844, 922
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 440
PaqCI CACCTGC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 2 cut(s) 468, 774
PdiI GCCGGC 2 cut(s) 292, 985
PfeI GAWTC 3 cut(s) 418, 628, 945
PflMI CCANNNNNTGG 1 cut(s) 820
PinAI ACCGGT 2 cut(s) 533, 1061
Ple19I CGATCG 2 cut(s) 214, 597
PleI GAGTC 2 cut(s) 543, 641
PpsI GAGTC 2 cut(s) 543, 641
PpuMI RGGWCCY 3 cut(s) 435, 959, 1068
Psp124BI GAGCTC 1 cut(s) 310
Psp5II RGGWCCY 3 cut(s) 435, 959, 1068
Psp6I CCWGG 2 cut(s) 229, 1024
PspGI CCWGG 2 cut(s) 229, 1024
PspPI GGNCC 5 cut(s) 344, 435, 518, 959, 1068
PspPPI RGGWCCY 3 cut(s) 435, 959, 1068
PstNI CAGNNNCTG 2 cut(s) 284, 820
PvuI CGATCG 2 cut(s) 214, 597
RsaI GTAC 4 cut(s) 264, 538, 590, 832
RsaNI GTAC 4 cut(s) 263, 537, 589, 831
RseI CAYNNNNRTG 2 cut(s) 270, 560
SacI GAGCTC 1 cut(s) 310
SacII CCGCGG 1 cut(s) 793
SalI GTCGAC 1 cut(s) 1049
SaqAI TTAA 3 cut(s) 366, 608, 740
Sau3AI GATC 3 cut(s) 211, 457, 594
Sau96I GGNCC 5 cut(s) 344, 435, 518, 959, 1068
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 2 cut(s) 543, 641
ScrFI CCNGG 4 cut(s) 231, 685, 957, 1026
SduI GDGCHC 5 cut(s) 181, 199, 229, 310, 705
SfaNI GCATC 4 cut(s) 17, 265, 564, 576
Sfr274I CTCGAG 1 cut(s) 440
Sfr303I CCGCGG 1 cut(s) 793
SgrBI CCGCGG 1 cut(s) 793
SinI GGWCC 4 cut(s) 435, 518, 959, 1068
SlaI CTCGAG 1 cut(s) 440
SmiMI CAYNNNNRTG 2 cut(s) 270, 560
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 4 cut(s) 363, 506, 707, 741
SspMI CTAG 1 cut(s) 546
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 4 cut(s) 229, 683, 955, 1024
TaaI ACNGT 3 cut(s) 700, 830, 850
TaiI ACGT 5 cut(s) 190, 270, 417, 465, 492
TaqI TCGA 6 cut(s) 214, 441, 495, 593, 978, 1050
TasI AATT 4 cut(s) 363, 506, 707, 741
TatI WGTACW 1 cut(s) 588
TauI GCSGC 5 cut(s) 55, 141, 297, 470, 792
TfiI GAWTC 3 cut(s) 418, 628, 945
Tru1I TTAA 3 cut(s) 366, 608, 740
Tru9I TTAA 3 cut(s) 366, 608, 740
TscAI CASTG 2 cut(s) 520, 946
TseFI GTSAC 3 cut(s) 694, 844, 922
TseI GCWGC 6 cut(s) 5, 59, 278, 287, 297, 914
Tsp45I GTSAC 3 cut(s) 694, 844, 922
TspDTI ATGAA 2 cut(s) 1093, 1112
TspGWI ACGGA 2 cut(s) 354, 464
TspRI CASTG 2 cut(s) 520, 946
Van91I CCANNNNNTGG 1 cut(s) 820
VneI GTGCAC 3 cut(s) 177, 225, 701
VpaK11BI GGWCC 4 cut(s) 435, 518, 959, 1068
XcmI CCANNNNNNNNNTGG 3 cut(s) 512, 713, 868
XhoI CTCGAG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 1050
XspI CTAG 1 cut(s) 546
ZraI GACGTC 1 cut(s) 490
ZrmI AGTACT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.