pycom07g28180

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005922
Physical Location & Seq
Forward (+)
27603311 .. 27604504
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g28180.1

Sequence Viewer

Length: 1194 bp
ATGGCAGCATCATCTTCCCCCCCAGTGGGGGCTAGTCGCCTGGGGAACCGGCGGCTTGCAGCTCTCGCTCTCTGCCTTGTATTGCTCATCGATATTACTACCACTACGTTGGTATTGGCTTCCAACAAGGGAAGAGGCCGCGTTGCAAATAAAGGTGCTGCAAAGAAAGGTGGTGGTGCACACAGAGGTGGTGGCACACACAAAGGTGGCGGTGCACACAGAGGTGGTGGTGCACAAAAAGGTAGCGGTCGAAAAGGTGGTGCACCTGTCACTGCTGGCGGCAAACCCGGTGGTGTCGTACACGCTGGTGGTTTAGCACCTGCTGCCGGCGGCAGCGTCCCAGCTCCTGGAGGTGGCGGACCCATCACATACCACGGAGGGCCTCTGATGACCGGCGACATTAACCTTTCCATCCTATTTTACGGCCAGTTCACAGCCGAACAAAAGAACGTGATCCGGAGTTTCCTGAGGTCCCTCGAGAACACGGAGAACGATCACGTCGCCGCCGTGCACAGGTGGTGGGACGTCATCGAGAGCTACCAATTGTTCGCTGGTCCAAACCCCGGTAAAGGTACCCCACCCAGACTCCGCATCAAGGTGGGGACCCAGCAGAGCGACGACAAGTACTCGATCGGAAAAGTGATAACCTTTGAGTTCATCAAGAGACTGGTGAATAAGGCCGGCTCCGGAAAGCCCAACACTATTAACGTGATCTTCACCGGGAGCCAGGTGACGGTGCACAGCTTGTGCCGTGGCAAATGCTATGAGCACGGCTTGATTGAGAACAGACCCTACCTTGTCGTCGGAAACCCCGAAATCGAGTGTCCCGGGGCTTGCGGTTGGCCATTCCAGAGGCTGGACTACGGTACGCCTAATCAAGTGACGGTGAAGCCCCCCAACGGCAACATGGGCATTGACGCCATGCTCTTAAACTTCGCCTCCGGCTTGGCTGCCACCGTCACGAACCCATTCAACACTGGATTCTTCAAGCCGGGACGAAAGAGCGACCCGGTCGAGGCCGGCACCGCGTGCGAAAACATCTTCGGAAGCGGCGCCGTCCCAGGGAAGACCGGCAAGGTTGAGGTCGACCGCGCTACTGGTGGGTCTTACAATGCCGTGGGAGAGAAGGGCATGAAGTTCATGCTCCCCGCCGTCTGGAACCCACGAACTTCCTCCTGCTGGACGGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

398

Amino Acids

41.13

Weight (kDa)

9.87

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 122 - 395 1.2e-89 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
AatII GACGTC 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 328
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 3 cut(s) 572, 1022, 1052
AccB7I CCANNNNNTGG 2 cut(s) 347, 856
AccI GTMKAC 1 cut(s) 1086
AccII CGCG 3 cut(s) 141, 1028, 1092
AccIII TCCGGA 2 cut(s) 456, 686
AclWI GGATC 1 cut(s) 448
AcoI YGGCCR 3 cut(s) 424, 842, 1185
AcyI GRCGYC 3 cut(s) 525, 918, 1053
AdeI CACNNNGTG 1 cut(s) 1029
AfaI GTAC 4 cut(s) 300, 574, 626, 868
AgsI TTSAA 2 cut(s) 973, 988
AjiI CACGTC 1 cut(s) 499
AjnI CCWGG 4 cut(s) 39, 346, 726, 1060
AleI CACNNNNGTG 4 cut(s) 186, 204, 222, 306
AluBI AGCT 4 cut(s) 62, 344, 537, 744
AluI AGCT 4 cut(s) 62, 344, 537, 744
Alw21I GWGCWC 7 cut(s) 181, 217, 235, 265, 513, 741, 771
Alw26I GTCTC 1 cut(s) 658
Alw44I GTGCAC 6 cut(s) 177, 213, 231, 261, 509, 737
AlwI GGATC 1 cut(s) 448
AlwNI CAGNNNCTG 2 cut(s) 347, 856
Ama87I CYCGRG 2 cut(s) 476, 827
Aor13HI TCCGGA 2 cut(s) 456, 686
AoxI GGCC 7 cut(s) 136, 380, 424, 678, 842, 1017, 1185
ApaLI GTGCAC 6 cut(s) 177, 213, 231, 261, 509, 737
ApeKI GCWGC 6 cut(s) 5, 59, 158, 323, 333, 950
Asp700I GAANNNNTTC 1 cut(s) 968
Asp718I GGTACC 1 cut(s) 572
AspLEI GCGC 2 cut(s) 1055, 1094
AspS9I GGNCC 5 cut(s) 359, 380, 471, 554, 603
AsuC2I CCSGG 7 cut(s) 288, 564, 721, 828, 829, 993, 1010
AsuHPI GGTGA 4 cut(s) 682, 709, 742, 898
AvaI CYCGRG 2 cut(s) 476, 827
AvaII GGWCC 4 cut(s) 359, 471, 554, 603
AxyI CCTNAGG 1 cut(s) 467
BaeGI GKGCMC 6 cut(s) 181, 217, 235, 265, 513, 741
BalI TGGCCA 1 cut(s) 844
BanI GGYRCC 3 cut(s) 572, 1022, 1052
BbsI GAAGAC 1 cut(s) 1073
Bbv12I GWGCWC 7 cut(s) 181, 217, 235, 265, 513, 741, 771
BbvI GCAGC 6 cut(s) 17, 71, 145, 310, 345, 937
BccI CCATC 2 cut(s) 371, 419
BceAI ACGGC 8 cut(s) 439, 491, 735, 787, 916, 1040, 1100, 1136
BciT130I CCWGG 4 cut(s) 41, 348, 728, 1062
BcnI CCSGG 7 cut(s) 288, 564, 721, 828, 829, 993, 1010
BcoDI GTCTC 1 cut(s) 658
BfaI CTAG 1 cut(s) 33
BfoI RGCGCY 1 cut(s) 1056
BfuAI ACCTGC 1 cut(s) 328
BmcAI AGTACT 1 cut(s) 626
Bme18I GGWCC 4 cut(s) 359, 471, 554, 603
BmeT110I CYCGRG 2 cut(s) 476, 827
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 5 cut(s) 359, 380, 471, 554, 603
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 2 cut(s) 17, 600
BmuI ACTGGG 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 1073
BpmI CTGGAG 1 cut(s) 369
BpuMI CCSGG 7 cut(s) 288, 564, 721, 828, 829, 993, 1010
Bsa29I ATCGAT 1 cut(s) 90
BsaHI GRCGYC 3 cut(s) 525, 918, 1053
BsaJI CCNNGG 9 cut(s) 40, 373, 562, 751, 827, 828, 1060, 1061, 1116
BsaWI WCCGGW 2 cut(s) 456, 686
BsaXI ACNNNNNCTCC 4 cut(s) 570, 600, 923, 953
Bse118I RCCGGY 6 cut(s) 48, 326, 392, 680, 1019, 1070
Bse1I ACTGG 5 cut(s) 23, 427, 672, 982, 1102
Bse21I CCTNAGG 1 cut(s) 467
BseAI TCCGGA 2 cut(s) 456, 686
BseBI CCWGG 4 cut(s) 41, 348, 728, 1062
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 9 cut(s) 40, 373, 562, 751, 827, 828, 1060, 1061, 1116
BseGI GGATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 5 cut(s) 23, 427, 672, 982, 1102
BseSI GKGCMC 6 cut(s) 181, 217, 235, 265, 513, 741
BseXI GCAGC 6 cut(s) 17, 71, 145, 310, 345, 937
BseYI CCCAGC 2 cut(s) 340, 606
Bsh1236I CGCG 3 cut(s) 141, 1028, 1092
Bsh1285I CGRYCG 4 cut(s) 250, 633, 1014, 1090
BshFI GGCC 7 cut(s) 138, 382, 426, 680, 844, 1019, 1187
BshNI GGYRCC 3 cut(s) 572, 1022, 1052
BshVI ATCGAT 1 cut(s) 90
BsiEI CGRYCG 4 cut(s) 250, 633, 1014, 1090
BsiHKAI GWGCWC 7 cut(s) 181, 217, 235, 265, 513, 741, 771
BsiHKCI CYCGRG 2 cut(s) 476, 827
BslFI GGGAC 7 cut(s) 323, 457, 536, 616, 810, 1008, 1043
BsmAI GTCTC 1 cut(s) 658
BsmFI GGGAC 7 cut(s) 323, 457, 536, 616, 810, 1008, 1043
BsnI GGCC 7 cut(s) 138, 382, 426, 680, 844, 1019, 1187
BsoBI CYCGRG 2 cut(s) 476, 827
Bsp1286I GDGCHC 7 cut(s) 181, 217, 235, 265, 513, 741, 771
Bsp13I TCCGGA 2 cut(s) 456, 686
Bsp143I GATC 4 cut(s) 453, 493, 630, 711
BspANI GGCC 7 cut(s) 138, 382, 426, 680, 844, 1019, 1187
BspCNI CTCAG 1 cut(s) 459
BspDI ATCGAT 1 cut(s) 90
BspEI TCCGGA 2 cut(s) 456, 686
BspFNI CGCG 3 cut(s) 141, 1028, 1092
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 1 cut(s) 448
BspT107I GGYRCC 3 cut(s) 572, 1022, 1052
BsrFI RCCGGY 6 cut(s) 48, 326, 392, 680, 1019, 1070
BsrI ACTGG 5 cut(s) 23, 427, 672, 982, 1102
BssAI RCCGGY 6 cut(s) 48, 326, 392, 680, 1019, 1070
BssECI CCNNGG 9 cut(s) 40, 373, 562, 751, 827, 828, 1060, 1061, 1116
BssMI GATC 4 cut(s) 453, 493, 630, 711
BssNI GRCGYC 3 cut(s) 525, 918, 1053
Bst2UI CCWGG 4 cut(s) 41, 348, 728, 1062
Bst4CI ACNGT 4 cut(s) 736, 866, 886, 958
Bst6I CTCTTC 1 cut(s) 127
BstACI GRCGYC 3 cut(s) 525, 918, 1053
BstAPI GCANNNNNTGC 2 cut(s) 323, 1029
BstC8I GCNNGC 7 cut(s) 57, 277, 328, 682, 835, 1021, 1030
BstDEI CTNAG 1 cut(s) 467
BstDSI CCRYGG 3 cut(s) 373, 751, 1116
BstF5I GGATG 1 cut(s) 411
BstFNI CGCG 3 cut(s) 141, 1028, 1092
BstH2I RGCGCY 1 cut(s) 1056
BstHHI GCGC 2 cut(s) 1055, 1094
BstKTI GATC 4 cut(s) 456, 496, 633, 714
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 4 cut(s) 453, 493, 630, 711
BstMCI CGRYCG 4 cut(s) 250, 633, 1014, 1090
BstMWI GCNNNNNNNGC 5 cut(s) 65, 323, 909, 1025, 1029
BstNI CCWGG 4 cut(s) 41, 348, 728, 1062
BstSLI GKGCMC 6 cut(s) 181, 217, 235, 265, 513, 741
BstUI CGCG 3 cut(s) 141, 1028, 1092
BstV1I GCAGC 6 cut(s) 17, 71, 145, 310, 345, 937
BstV2I GAAGAC 1 cut(s) 1073
BstXI CCANNNNNNTGG 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 90
Bsu36I CCTNAGG 1 cut(s) 467
BsuRI GGCC 7 cut(s) 138, 382, 426, 680, 844, 1019, 1187
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 3 cut(s) 373, 751, 1116
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 1 cut(s) 411
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 3 cut(s) 30, 270, 975
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 7 cut(s) 57, 277, 328, 682, 835, 1021, 1030
CaiI CAGNNNCTG 2 cut(s) 347, 856
CfoI GCGC 2 cut(s) 1055, 1094
Cfr10I RCCGGY 6 cut(s) 48, 326, 392, 680, 1019, 1070
Cfr13I GGNCC 5 cut(s) 359, 380, 471, 554, 603
Cfr9I CCCGGG 1 cut(s) 827
ClaI ATCGAT 1 cut(s) 90
CseI GACGC 2 cut(s) 325, 926
Csp6I GTAC 4 cut(s) 299, 573, 625, 867
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 5 cut(s) 907, 922, 1132, 1141, 1189
CviQI GTAC 4 cut(s) 299, 573, 625, 867
DdeI CTNAG 1 cut(s) 467
DinI GGCGCC 1 cut(s) 1054
DpnI GATC 4 cut(s) 455, 495, 632, 713
DpnII GATC 4 cut(s) 453, 493, 630, 711
DraIII CACNNNGTG 1 cut(s) 1029
EaeI YGGCCR 3 cut(s) 424, 842, 1185
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
EciI GGCGGA 1 cut(s) 372
Eco47I GGWCC 4 cut(s) 359, 471, 554, 603
Eco81I CCTNAGG 1 cut(s) 467
Eco88I CYCGRG 2 cut(s) 476, 827
EcoO109I RGGNCCY 3 cut(s) 380, 471, 603
EcoRII CCWGG 4 cut(s) 39, 346, 726, 1060
EgeI GGCGCC 1 cut(s) 1054
EheI GGCGCC 1 cut(s) 1054
FaeI CATG 5 cut(s) 910, 925, 1135, 1144, 1192
FaiI YATR 7 cut(s) 370, 765, 908, 923, 1133, 1142, 1190
FaqI GGGAC 7 cut(s) 323, 457, 536, 616, 810, 1008, 1043
FatI CATG 5 cut(s) 906, 921, 1131, 1140, 1188
FauI CCCGC 1 cut(s) 1156
FblI GTMKAC 1 cut(s) 1086
FokI GGATG 1 cut(s) 398
FspBI CTAG 1 cut(s) 33
GlaI GCGC 2 cut(s) 1054, 1093
GsaI CCCAGC 2 cut(s) 344, 610
GsuI CTGGAG 1 cut(s) 369
HaeII RGCGCY 1 cut(s) 1056
HaeIII GGCC 7 cut(s) 138, 382, 426, 680, 844, 1019, 1187
HgaI GACGC 2 cut(s) 325, 926
HhaI GCGC 2 cut(s) 1055, 1094
Hin1I GRCGYC 3 cut(s) 525, 918, 1053
Hin1II CATG 5 cut(s) 910, 925, 1135, 1144, 1192
Hin6I GCGC 2 cut(s) 1053, 1092
HinP1I GCGC 2 cut(s) 1053, 1092
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HinfI GANTC 2 cut(s) 585, 981
HphI GGTGA 4 cut(s) 682, 709, 742, 898
Hpy166II GTNNAC 9 cut(s) 179, 215, 233, 263, 301, 432, 511, 739, 1087
Hpy188I TCNGA 4 cut(s) 387, 635, 806, 1046
Hpy188III TCNNGA 9 cut(s) 457, 466, 478, 532, 661, 687, 850, 961, 1156
Hpy8I GTNNAC 9 cut(s) 179, 215, 233, 263, 301, 432, 511, 739, 1087
Hpy99I CGWCG 3 cut(s) 503, 620, 806
HpyAV CCTTC 1 cut(s) 1120
HpyCH4III ACNGT 4 cut(s) 736, 866, 886, 958
HpyCH4IV ACGT 5 cut(s) 107, 450, 498, 525, 708
HpyCH4V TGCA 9 cut(s) 59, 146, 161, 179, 215, 233, 263, 511, 739
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 323, 909, 1025, 1029
HpyF3I CTNAG 1 cut(s) 467
HpySE526I ACGT 5 cut(s) 107, 450, 498, 525, 708
Hsp92I GRCGYC 3 cut(s) 525, 918, 1053
Hsp92II CATG 5 cut(s) 910, 925, 1135, 1144, 1192
HspAI GCGC 2 cut(s) 1053, 1092
KasI GGCGCC 1 cut(s) 1052
KflI GGGWCCC 1 cut(s) 603
Kpn2I TCCGGA 2 cut(s) 456, 686
KpnI GGTACC 1 cut(s) 576
KroI GCCGGC 3 cut(s) 326, 680, 1019
KroNI GCCGGC 3 cut(s) 328, 682, 1021
Kzo9I GATC 4 cut(s) 453, 493, 630, 711
LmnI GCTCC 4 cut(s) 349, 689, 723, 1149
Lsp1109I GCAGC 6 cut(s) 17, 71, 145, 310, 345, 937
LweI GCATC 2 cut(s) 17, 600
MaeI CTAG 1 cut(s) 33
MaeII ACGT 5 cut(s) 107, 450, 498, 525, 708
MaeIII GTNAC 4 cut(s) 268, 730, 880, 958
MalI GATC 4 cut(s) 455, 495, 632, 713
MboI GATC 4 cut(s) 453, 493, 630, 711
MboII GAAGA 6 cut(s) 6, 144, 706, 976, 1033, 1078
MfeI CAATTG 1 cut(s) 542
MhlI GDGCHC 7 cut(s) 181, 217, 235, 265, 513, 741, 771
MlsI TGGCCA 1 cut(s) 844
MluCI AATT 1 cut(s) 542
MluNI TGGCCA 1 cut(s) 844
Mly113I GGCGCC 1 cut(s) 1053
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 2 cut(s) 147, 784
Mox20I TGGCCA 1 cut(s) 844
MroI TCCGGA 2 cut(s) 456, 686
MroNI GCCGGC 3 cut(s) 326, 680, 1019
MroXI GAANNNNTTC 1 cut(s) 968
MscI TGGCCA 1 cut(s) 844
MseI TTAA 3 cut(s) 402, 705, 929
MslI CAYNNNNRTG 5 cut(s) 186, 204, 222, 306, 596
Msp20I TGGCCA 1 cut(s) 844
MunI CAATTG 1 cut(s) 542
MvaI CCWGG 4 cut(s) 41, 348, 728, 1062
MvnI CGCG 3 cut(s) 141, 1028, 1092
MwoI GCNNNNNNNGC 5 cut(s) 65, 323, 909, 1025, 1029
NaeI GCCGGC 3 cut(s) 328, 682, 1021
NarI GGCGCC 1 cut(s) 1053
NciI CCSGG 7 cut(s) 288, 564, 721, 828, 829, 993, 1010
NdeII GATC 4 cut(s) 453, 493, 630, 711
NgoMIV GCCGGC 3 cut(s) 326, 680, 1019
NlaIII CATG 5 cut(s) 910, 925, 1135, 1144, 1192
NmuCI GTSAC 4 cut(s) 268, 730, 880, 958
OliI CACNNNNGTG 4 cut(s) 186, 204, 222, 306
PaeR7I CTCGAG 1 cut(s) 476
PaqCI CACCTGC 1 cut(s) 328
PasI CCCWGGG 1 cut(s) 1061
PcsI WCGNNNNNNNCGW 2 cut(s) 504, 810
PdiI GCCGGC 3 cut(s) 328, 682, 1021
PdmI GAANNNNTTC 1 cut(s) 968
PfeI GAWTC 1 cut(s) 981
PflFI GACNNNGTC 1 cut(s) 1010
PflMI CCANNNNNTGG 2 cut(s) 347, 856
PfoI TCCNGGA 1 cut(s) 346
Ple19I CGATCG 1 cut(s) 633
PleI GAGTC 1 cut(s) 579
PluTI GGCGCC 1 cut(s) 1056
PpsI GAGTC 1 cut(s) 579
PpuMI RGGWCCY 2 cut(s) 471, 603
Psp5II RGGWCCY 2 cut(s) 471, 603
Psp6I CCWGG 4 cut(s) 39, 346, 726, 1060
PspFI CCCAGC 2 cut(s) 340, 606
PspGI CCWGG 4 cut(s) 39, 346, 726, 1060
PspPI GGNCC 5 cut(s) 359, 380, 471, 554, 603
PspPPI RGGWCCY 2 cut(s) 471, 603
PsrI GAACNNNNNNTAC 2 cut(s) 776, 808
PstNI CAGNNNCTG 2 cut(s) 347, 856
PsyI GACNNNGTC 1 cut(s) 1010
PvuI CGATCG 1 cut(s) 633
RsaI GTAC 4 cut(s) 300, 574, 626, 868
RsaNI GTAC 4 cut(s) 299, 573, 625, 867
RseI CAYNNNNRTG 5 cut(s) 186, 204, 222, 306, 596
SalI GTCGAC 1 cut(s) 1085
SaqAI TTAA 3 cut(s) 402, 705, 929
Sau3AI GATC 4 cut(s) 453, 493, 630, 711
Sau96I GGNCC 5 cut(s) 359, 380, 471, 554, 603
ScaI AGTACT 1 cut(s) 626
SchI GAGTC 1 cut(s) 579
SduI GDGCHC 7 cut(s) 181, 217, 235, 265, 513, 741, 771
SfaNI GCATC 2 cut(s) 17, 600
SfoI GGCGCC 1 cut(s) 1054
Sfr274I CTCGAG 1 cut(s) 476
SinI GGWCC 4 cut(s) 359, 471, 554, 603
SlaI CTCGAG 1 cut(s) 476
SmaI CCCGGG 1 cut(s) 829
SmiMI CAYNNNNRTG 5 cut(s) 186, 204, 222, 306, 596
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 1 cut(s) 542
SspDI GGCGCC 1 cut(s) 1052
SspMI CTAG 1 cut(s) 33
TaaI ACNGT 4 cut(s) 736, 866, 886, 958
TaiI ACGT 5 cut(s) 110, 453, 501, 528, 711
TaqI TCGA 8 cut(s) 90, 250, 477, 531, 629, 819, 1014, 1086
TasI AATT 1 cut(s) 542
TatI WGTACW 1 cut(s) 624
TauI GCSGC 6 cut(s) 55, 141, 282, 333, 506, 1053
TfiI GAWTC 1 cut(s) 981
Tru1I TTAA 3 cut(s) 402, 705, 929
Tru9I TTAA 3 cut(s) 402, 705, 929
TscAI CASTG 3 cut(s) 30, 277, 982
TseFI GTSAC 4 cut(s) 268, 730, 880, 958
TseI GCWGC 6 cut(s) 5, 59, 158, 323, 333, 950
Tsp45I GTSAC 4 cut(s) 268, 730, 880, 958
TspDTI ATGAA 3 cut(s) 646, 1129, 1148
TspGWI ACGGA 2 cut(s) 390, 500
TspMI CCCGGG 1 cut(s) 827
TspRI CASTG 3 cut(s) 30, 277, 982
Tth111I GACNNNGTC 1 cut(s) 1010
Van91I CCANNNNNTGG 2 cut(s) 347, 856
VneI GTGCAC 6 cut(s) 177, 213, 231, 261, 509, 737
VpaK11BI GGWCC 4 cut(s) 359, 471, 554, 603
XcmI CCANNNNNNNNNTGG 2 cut(s) 548, 904
XhoI CTCGAG 1 cut(s) 476
XmaI CCCGGG 1 cut(s) 827
XmiI GTMKAC 1 cut(s) 1086
XmnI GAANNNNTTC 1 cut(s) 968
XspI CTAG 1 cut(s) 33
ZraI GACGTC 1 cut(s) 526
ZrmI AGTACT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.