Rroxscaffold_4G00276720

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005922
Physical Location & Seq
Reverse (-)
232407 .. 233339
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276720.1

Sequence Viewer

Length: 933 bp
ATGGCCTTATCAAGATCAAGAGTTCATCCTGATCAGCTTGCTCTGGTTCTCAGTTTACTCTTGTTAATTACTACTCCATCCCTGGTTTTGGGGCACGTTAAACCCCCAGGCACCATCTGTTACAATGGAGGGCTACTCCTGACCGGCAAACTCGACCTTTCAATCTTCTTCTATGGTGCAATTGGGCGCACACAAAAGCTTGTCGTCAGGAATTTCATCAAGTCCCTAAATCATGGTGGTGGCACCCTTAAGCCTCGGGTAACATCCTGGTGGGACATGGTCGAGAGCTACCAATCCGTATCTTCCCCCGATCATAAAGCGCCCAAAATCGCAGTTAAAGTGGTGAGTCAAATAACCGAGAAAGGTTACTCCCTGGGGAAGGTGATCACCATTGACTTCATAAGGTCGATGGTCAAAAAGGCCACCGCCGGGAAGCCTCCTGCCTCTCTTGTCGTCATATTCACCGATAAGGAAAGTGCGGTCGCATCCCTTTGCAGGGGAAAATGCTATGATCACGGAGTCACAGATAACCAGCCCTATCTCATCGTCGGAAACCCCGAAGTCGAGTGCCCTGATGCTTGTGGCTGGCCCTTCATGAAGTCCAGCTACAATCCAGGCATCACCCTCACACCGCCAAACGGCAACCCCGCCGTCGATGCTATTCTAGTCAACTTTGCTGCCGGTTTGGCTGCTGCCGTCACCAATCCATTCAACACCGGATTTTCCAAACCAGGACCCAAAACCTGGCCGCTGGAGGCCAGCACTGTTTGTGACGGTATATTCGCAAGCGGGTCGTTCCCGGGGAATCCCGGCAAGGTGGCGATGGACGCGAGAACAGGTGCTGCTTTCAATGCTCATGGATTGAAGGGTATGAAGTTTTTGCTTCCTGCGCTGTGGAGTCCACGAACATCATCATGCTGGACCCCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

310

Amino Acids

33.02

Weight (kDa)

9.43

Isoelectric Point (pI)

23.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 41 - 309 6.6e-79 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 110, 242
AccB7I CCANNNNNTGG 1 cut(s) 744
AccII CGCG 1 cut(s) 830
AciI CCGC 6 cut(s) 426, 479, 632, 648, 749, 789
AcoI YGGCCR 1 cut(s) 746
AcsI RAATTY 1 cut(s) 211
AfiI CCNNNNNNNGG 7 cut(s) 88, 379, 429, 495, 496, 638, 744
AflII CTTAAG 1 cut(s) 248
AgsI TTSAA 4 cut(s) 162, 712, 850, 865
AjnI CCWGG 7 cut(s) 81, 106, 266, 372, 613, 730, 743
AluBI AGCT 4 cut(s) 37, 199, 288, 606
AluI AGCT 4 cut(s) 37, 199, 288, 606
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 2 cut(s) 255, 799
AoxI GGCC 5 cut(s) 3, 420, 587, 746, 756
ApeKI GCWGC 4 cut(s) 677, 689, 692, 842
ApoI RAATTY 1 cut(s) 211
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
AspLEI GCGC 3 cut(s) 189, 322, 892
AspS9I GGNCC 3 cut(s) 588, 734, 921
AsuC2I CCSGG 4 cut(s) 430, 800, 801, 810
AsuHPI GGTGA 6 cut(s) 355, 379, 394, 454, 613, 691
AvaI CYCGRG 2 cut(s) 255, 799
AvaII GGWCC 2 cut(s) 734, 921
BaeGI GKGCMC 2 cut(s) 96, 572
BanI GGYRCC 2 cut(s) 110, 242
BarI GAAGNNNNNNTAC 2 cut(s) 590, 622
BbvI GCAGC 4 cut(s) 664, 676, 679, 829
BccI CCATC 4 cut(s) 85, 122, 403, 817
BceAI ACGGC 3 cut(s) 635, 655, 680
BcgI CGANNNNNNTGC 2 cut(s) 774, 808
BciT130I CCWGG 7 cut(s) 83, 108, 268, 374, 615, 732, 745
BclI TGATCA 3 cut(s) 31, 384, 511
BcnI CCSGG 4 cut(s) 430, 800, 801, 810
BfaI CTAG 1 cut(s) 665
BfoI RGCGCY 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 248
BglI GCCNNNNNGGC 1 cut(s) 686
BisI GCNGC 5 cut(s) 678, 690, 693, 749, 843
BlsI GCNGC 5 cut(s) 679, 691, 694, 750, 844
Bme18I GGWCC 2 cut(s) 734, 921
BmeT110I CYCGRG 2 cut(s) 255, 799
BmgT120I GGNCC 3 cut(s) 588, 734, 921
BmiI GGNNCC 4 cut(s) 112, 244, 736, 923
BmsI GCATC 4 cut(s) 494, 565, 627, 646
BplI GAGNNNNNCTC 2 cut(s) 120, 152
BpmI CTGGAG 1 cut(s) 773
BpuMI CCSGG 4 cut(s) 430, 800, 801, 810
BsaJI CCNNGG 7 cut(s) 81, 106, 254, 372, 373, 799, 800
BsaWI WCCGGW 1 cut(s) 716
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 379, 429, 495, 496, 638, 744
Bse118I RCCGGY 2 cut(s) 143, 680
BseBI CCWGG 7 cut(s) 83, 108, 268, 374, 615, 732, 745
BseDI CCNNGG 7 cut(s) 81, 106, 254, 372, 373, 799, 800
BseGI GGATG 4 cut(s) 25, 77, 263, 485
BseLI CCNNNNNNNGG 7 cut(s) 88, 379, 429, 495, 496, 638, 744
BseMII CTCAG 1 cut(s) 64
BseSI GKGCMC 2 cut(s) 96, 572
BseXI GCAGC 4 cut(s) 664, 676, 679, 829
Bsh1236I CGCG 1 cut(s) 830
Bsh1285I CGRYCG 1 cut(s) 483
BshFI GGCC 5 cut(s) 5, 422, 589, 748, 758
BshNI GGYRCC 2 cut(s) 110, 242
BsiEI CGRYCG 1 cut(s) 483
BsiHKCI CYCGRG 2 cut(s) 255, 799
BsiSI CCGG 6 cut(s) 144, 429, 681, 717, 800, 810
BslFI GGGAC 2 cut(s) 208, 287
BslI CCNNNNNNNGG 7 cut(s) 88, 379, 429, 495, 496, 638, 744
BsmFI GGGAC 2 cut(s) 208, 287
BsnI GGCC 5 cut(s) 5, 422, 589, 748, 758
BsoBI CYCGRG 2 cut(s) 255, 799
Bsp1286I GDGCHC 2 cut(s) 96, 572
Bsp143I GATC 5 cut(s) 14, 31, 310, 384, 511
BspACI CCGC 6 cut(s) 426, 479, 632, 648, 749, 789
BspANI GGCC 5 cut(s) 5, 422, 589, 748, 758
BspCNI CTCAG 1 cut(s) 63
BspFNI CGCG 1 cut(s) 830
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 4 cut(s) 112, 244, 736, 923
BspT107I GGYRCC 2 cut(s) 110, 242
BspTI CTTAAG 1 cut(s) 248
BsrFI RCCGGY 2 cut(s) 143, 680
BssAI RCCGGY 2 cut(s) 143, 680
BssECI CCNNGG 7 cut(s) 81, 106, 254, 372, 373, 799, 800
BssMI GATC 5 cut(s) 14, 31, 310, 384, 511
Bst2UI CCWGG 7 cut(s) 83, 108, 268, 374, 615, 732, 745
Bst4CI ACNGT 2 cut(s) 766, 776
BstAFI CTTAAG 1 cut(s) 248
BstC8I GCNNGC 4 cut(s) 39, 587, 760, 787
BstDEI CTNAG 1 cut(s) 50
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 4 cut(s) 25, 77, 263, 485
BstFNI CGCG 1 cut(s) 830
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 3 cut(s) 189, 322, 892
BstKTI GATC 5 cut(s) 17, 34, 313, 387, 514
BstMBI GATC 5 cut(s) 14, 31, 310, 384, 511
BstMCI CGRYCG 1 cut(s) 483
BstMWI GCNNNNNNNGC 5 cut(s) 656, 686, 827, 851, 889
BstNI CCWGG 7 cut(s) 83, 108, 268, 374, 615, 732, 745
BstSLI GKGCMC 2 cut(s) 96, 572
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 4 cut(s) 664, 676, 679, 829
BsuRI GGCC 5 cut(s) 5, 422, 589, 748, 758
BtgZI GCGATG 1 cut(s) 836
BtsCI GGATG 4 cut(s) 25, 77, 263, 485
BtsIMutI CAGTG 1 cut(s) 762
Cac8I GCNNGC 4 cut(s) 39, 587, 760, 787
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 1 cut(s) 594
CfoI GCGC 3 cut(s) 189, 322, 892
Cfr10I RCCGGY 2 cut(s) 143, 680
Cfr13I GGNCC 3 cut(s) 588, 734, 921
Cfr9I CCCGGG 1 cut(s) 799
CseI GACGC 1 cut(s) 836
CviAII CATG 5 cut(s) 233, 277, 595, 857, 915
DdeI CTNAG 1 cut(s) 50
DpnI GATC 5 cut(s) 16, 33, 312, 386, 513
DpnII GATC 5 cut(s) 14, 31, 310, 384, 511
EaeI YGGCCR 1 cut(s) 746
Eco47I GGWCC 2 cut(s) 734, 921
Eco88I CYCGRG 2 cut(s) 255, 799
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 734
EcoRII CCWGG 7 cut(s) 81, 106, 266, 372, 613, 730, 743
FaeI CATG 5 cut(s) 236, 280, 598, 860, 918
FaqI GGGAC 2 cut(s) 208, 287
FatI CATG 5 cut(s) 232, 276, 594, 856, 914
FauI CCCGC 2 cut(s) 655, 782
FbaI TGATCA 3 cut(s) 31, 384, 511
Fnu4HI GCNGC 5 cut(s) 678, 690, 693, 749, 843
FokI GGATG 4 cut(s) 12, 64, 250, 472
Fsp4HI GCNGC 5 cut(s) 678, 690, 693, 749, 843
FspBI CTAG 1 cut(s) 665
GlaI GCGC 3 cut(s) 188, 321, 891
GluI GCNGC 5 cut(s) 678, 690, 693, 749, 843
GsuI CTGGAG 1 cut(s) 773
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 5 cut(s) 5, 422, 589, 748, 758
HapII CCGG 6 cut(s) 144, 429, 681, 717, 800, 810
HgaI GACGC 1 cut(s) 836
HhaI GCGC 3 cut(s) 189, 322, 892
Hin1II CATG 5 cut(s) 236, 280, 598, 860, 918
Hin6I GCGC 3 cut(s) 187, 320, 890
HinP1I GCGC 3 cut(s) 187, 320, 890
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 4 cut(s) 346, 519, 805, 898
HpaII CCGG 6 cut(s) 144, 429, 681, 717, 800, 810
HphI GGTGA 6 cut(s) 355, 379, 394, 454, 613, 691
Hpy166II GTNNAC 3 cut(s) 56, 670, 902
Hpy188I TCNGA 1 cut(s) 551
Hpy188III TCNNGA 7 cut(s) 12, 18, 29, 139, 208, 283, 595
Hpy8I GTNNAC 3 cut(s) 56, 670, 902
Hpy99I CGWCG 2 cut(s) 551, 656
HpyAV CCTTC 3 cut(s) 373, 601, 859
HpyCH4III ACNGT 2 cut(s) 766, 776
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 2 cut(s) 179, 495
HpyF10VI GCNNNNNNNGC 5 cut(s) 656, 686, 827, 851, 889
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 5 cut(s) 236, 280, 598, 860, 918
HspAI GCGC 3 cut(s) 187, 320, 890
Ksp22I TGATCA 3 cut(s) 31, 384, 511
Kzo9I GATC 5 cut(s) 14, 31, 310, 384, 511
Lsp1109I GCAGC 4 cut(s) 664, 676, 679, 829
LweI GCATC 4 cut(s) 494, 565, 627, 646
MaeI CTAG 1 cut(s) 665
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 6 cut(s) 119, 259, 365, 520, 697, 770
MalI GATC 5 cut(s) 16, 33, 312, 386, 513
MboI GATC 5 cut(s) 14, 31, 310, 384, 511
MboII GAAGA 3 cut(s) 157, 160, 294
MfeI CAATTG 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 96, 572
MluCI AATT 3 cut(s) 66, 180, 211
MlyI GAGTC 3 cut(s) 355, 528, 907
MmeI TCCRAC 1 cut(s) 529
MnlI CCTC 6 cut(s) 122, 264, 447, 454, 635, 748
MseI TTAA 4 cut(s) 65, 99, 249, 336
MslI CAYNNNNRTG 3 cut(s) 237, 268, 913
MspA1I CMGCKG 1 cut(s) 751
MspCI CTTAAG 1 cut(s) 248
MspI CCGG 6 cut(s) 144, 429, 681, 717, 800, 810
MunI CAATTG 1 cut(s) 180
MvaI CCWGG 7 cut(s) 83, 108, 268, 374, 615, 732, 745
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 5 cut(s) 656, 686, 827, 851, 889
NciI CCSGG 4 cut(s) 430, 800, 801, 810
NdeII GATC 5 cut(s) 14, 31, 310, 384, 511
NlaIII CATG 5 cut(s) 236, 280, 598, 860, 918
NlaIV GGNNCC 4 cut(s) 112, 244, 736, 923
NmuCI GTSAC 3 cut(s) 520, 697, 770
PagI TCATGA 1 cut(s) 594
PasI CCCWGGG 1 cut(s) 373
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PfeI GAWTC 1 cut(s) 805
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 744
PkrI GCNGC 5 cut(s) 679, 691, 694, 750, 844
PleI GAGTC 3 cut(s) 354, 527, 906
PpsI GAGTC 3 cut(s) 354, 527, 906
PpuMI RGGWCCY 1 cut(s) 734
Psp5II RGGWCCY 1 cut(s) 734
Psp6I CCWGG 7 cut(s) 81, 106, 266, 372, 613, 730, 743
PspGI CCWGG 7 cut(s) 81, 106, 266, 372, 613, 730, 743
PspN4I GGNNCC 4 cut(s) 112, 244, 736, 923
PspPI GGNCC 3 cut(s) 588, 734, 921
PspPPI RGGWCCY 1 cut(s) 734
PstNI CAGNNNCTG 1 cut(s) 842
PsyI GACNNNGTC 1 cut(s) 278
RseI CAYNNNNRTG 3 cut(s) 237, 268, 913
SaqAI TTAA 4 cut(s) 65, 99, 249, 336
SatI GCNGC 5 cut(s) 678, 690, 693, 749, 843
Sau3AI GATC 5 cut(s) 14, 31, 310, 384, 511
Sau96I GGNCC 3 cut(s) 588, 734, 921
SchI GAGTC 3 cut(s) 355, 528, 907
SduI GDGCHC 2 cut(s) 96, 572
SfaNI GCATC 4 cut(s) 494, 565, 627, 646
SinI GGWCC 2 cut(s) 734, 921
SmaI CCCGGG 1 cut(s) 801
SmiMI CAYNNNNRTG 3 cut(s) 237, 268, 913
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 3 cut(s) 66, 180, 211
SsiI CCGC 6 cut(s) 426, 479, 632, 648, 749, 789
SspMI CTAG 1 cut(s) 665
TaaI ACNGT 2 cut(s) 766, 776
TaiI ACGT 1 cut(s) 99
TaqI TCGA 5 cut(s) 153, 282, 407, 564, 654
TasI AATT 3 cut(s) 66, 180, 211
TauI GCSGC 1 cut(s) 751
TfiI GAWTC 1 cut(s) 805
Tru1I TTAA 4 cut(s) 65, 99, 249, 336
Tru9I TTAA 4 cut(s) 65, 99, 249, 336
TscAI CASTG 1 cut(s) 769
TseFI GTSAC 3 cut(s) 520, 697, 770
TseI GCWGC 4 cut(s) 677, 689, 692, 842
Tsp45I GTSAC 3 cut(s) 520, 697, 770
TspDTI ATGAA 6 cut(s) 14, 205, 388, 583, 611, 887
TspGWI ACGGA 2 cut(s) 286, 531
TspMI CCCGGG 1 cut(s) 799
TspRI CASTG 1 cut(s) 769
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 744
Vha464I CTTAAG 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 734, 921
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 1 cut(s) 211
XmaI CCCGGG 1 cut(s) 799
XspI CTAG 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.