Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002722

Physical Location & Seq

Genomic Coordinates Forward (+)

11771379 .. 11774961

Gene Structure 3583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1125200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGGCACATATTCACAAAGCATCATATGATCACCATCACTGGCTTTTCATGCTAGTTTTCTTAGTACTATCATACGTTGCTTCCTCTTCAGATCCGACCGAGCCAGCCATCCTCCTCAAGTTCAAGTCATTTCTATCCAACGACAGTGCTTTGGACAACTGGACTATCGCCACAGCTCCCTGCAACGGTACGCACAACCTCTGGGCAGGTTTGCTTTGTAATGGCGACGGCAATGTGTACGGGTTGGCTTTAACAAACATGGGTCTCCTGGGGATAATCGATATAGACACACTTATGGATTTGTCTGCCCTGAAAACCATAAGCTTCATGAACAATAGCTTTGAAGGTCCTCTCCCTAGTGTGAACAAACTTAGTTCACTTACATCAGTTTACTTGTCGCACAATAAATTCAATGGTGAAATCCCAGATAATTTTTTTGTTGGCATGAATTCCTTGAAGAAAATTTATCTGGATGGGAATGAATTCACGGGTAAAATACCAAAATCGCTGGCTGATTTGACTAAGATTGTGGAAATGGATATTCGGGGAAACCAATTCAGTGGGAAACTACCAAATTTTCCGCAAAATCCAAATGCATGGAAGACTTTGAATGTGTCATACAACAGACTCGAGGGTCGTATTCCGGCAGGCCTGAGAGTTTCCAACCCAAGCGCTTTCGTCGGAAACCCAGACTTATGTGGGAAGCCACTGGCTGCATGCAAGTCCTTAAAGAAGAAAGTCCTTCTAATCATTGCCATCTTAGTCATCTCAGTTGCTGCAATCTTGTTCTTCATTGTAGTATGCCTCAAACGACGCCGTTCAGCACAATCAAAACAAGTGACAACAGTACAAAACAAACTGGGGGCCAACCATGACACAGGCGCAATGGAGGTCGAATTGGCAGCTGAAGAAGAAAATAACAACTTCAAGAACAATGCTGAGAGAGGAGGGGGGGAGTTGTATTTTCTGAAGGACATAAATTTTGAGTTGGAAGAGTTGCTGAGGGCCCCAGCTGAGGTTTTGGGAAGTGGGTCCTTTGGGTCATCTTACAAGGCAGGGCTTTTGAGTGGGTCCATGGTTGTGAAGAGGTTTAAGGAAATGAACCAAGTGAGGAAAGACGACTTTTATGACCACATGAGAAGGCTTGGAAGGTTGTCACACCCTAATTTGCTCCCACTTGTTGCTTTCTATTATAGGAAAGAAGAGAAGCTCTTGGTTCATGATTTTGTTGCCAATGGAAGCTTGGCTAGTCATCTCCATGCTAAGCGAGAACCAGGGCAGCCGGGATTGAATTGGCCAACTCGGTTGATGATCATCAAAGGAGTGGCAAGGGGTTTGGCCTACCTCTACAAAGAGTTCCCCGGCCTAACGTTACCCCACGGTCACCTCAAGTCTTCCAACGTGCTCCTAGACCACAACTTCAATCCTCTCCTGGCAGAGTATGCCCTAATACCCCTAGTCAACACAGACCATGCCCAAAAGTTCATGGTGGCTTTCAAGTCCCCGGAGTTCTATCATAACGAACGCTCGTCAAAAAAGACCGATGTATGGAGCCTAGGCATACTCATCTTCGAAATGCTCACCGGAAAATTCCCAGCAAACTATCTCCAACCCGGGAAGAGAGCGAACGCGGACTTAGCTGCTTGGGTGAACTCAGTTGTGAGAGAAGAGTGGACGGGTGAAGTGTTTGACAAGGACATGAAGGGGACGACGAACGGAGAAGGTGAGATGCTGAAGATGCTGAAGATTGGGATGTGTTGCTGTGAGAGCAGTGTGGAGAGGAGGTGGGATTGGAGAGAAGTTGTGGACAAGATTGAAGAGTTAAAGGAGAGGGACGGTGAAGAAGACTACTCATCTTATGCTAGTGATGGGGATGTGTACTCTTCTAGGGCTATTTCTGAGGACGATTTCTCTTTCTCTGTTAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

644

Amino Acids

71.8

Weight (kDa)

6.82

Isoelectric Point (pI)

30.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	126 - 186	4.2e-09	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	339 - 536	9.8e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	339 - 601	1.4e-23	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002722)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50610 AT3G20190 AT3G20190
fragaria_vesca	FvH4_2g36521 FvH4_2g36530 FvH4_6g36540
malus_domestica	MD08G1125100.v1.1 MD08G1125200.v1.1 MD09G1167500.v1.1 MD15G1104700.v1.1 MD17G1160800.v1.1
prunus_persica	Prupe.1G456700_v2.0.a1 Prupe.1G456800_v2.0.a1 Prupe.3G025500_v2.0.a1
pyrus_communis	pycom08g10400 pycom09g08500 pycom15g09530 pycom17g15310
rosa_chinensis	RchiOBHm_Chr2g0149011 RchiOBHm_Chr6g0305321 RchiOBHm_Chr6g0305341
rosa_laevigata	RLG00000010898 RLG00000010899 RLG00000020362
rosa_multiflora	Rmu_sc0007360.1_g000007 Rmu_ssc0000148.1_g000003
rosa_roxburghii	Rroxscaffold_7G00162890 Rroxscaffold_7G00162900
rosa_rugosa	Rorug02G0408600 Rorug06G0339600 Rorug06G0339700.1
rosa_samantha	Rh2AG467900 Rh2BG480700 Rh2CG454300 Rh2DG489600 Rh6AG451200 Rh6AG451400
rosa_wichuraiana	Rw2G038120 Rw6G039330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	633
Acc36I	ACCTGC	1 cut(s)	197
AccII	CGCG	1 cut(s)	1631
AciI	CCGC	2 cut(s)	581, 1631
AclI	AACGTT	1 cut(s)	1370
AclWI	GGATC	1 cut(s)	86
AcoI	YGGCCR	1 cut(s)	1295
AcsI	RAATTY	7 cut(s)	407, 448, 462, 482, 574, 979, 1589
AcuI	CTGAAG	5 cut(s)	72, 927, 989, 1754, 1763
AcyI	GRCGYC	1 cut(s)	814
AfaI	GTAC	5 cut(s)	66, 190, 239, 849, 1880
AfeI	AGCGCT	1 cut(s)	673
AfiI	CCNNNNNNNGG	3 cut(s)	185, 1015, 1548
AjnI	CCWGG	3 cut(s)	267, 1273, 1431
AluBI	AGCT	8 cut(s)	176, 324, 339, 905, 1013, 1210, 1242, 1640
AluI	AGCT	8 cut(s)	176, 324, 339, 905, 1013, 1210, 1242, 1640
Alw21I	GWGCWC	1 cut(s)	1407
Alw26I	GTCTC	1 cut(s)	269
AlwI	GGATC	1 cut(s)	86
AlwNI	CAGNNNCTG	1 cut(s)	776
Ama87I	CYCGRG	2 cut(s)	629, 1613
Aor51HI	AGCGCT	1 cut(s)	673
AoxI	GGCC	6 cut(s)	649, 864, 1005, 1295, 1338, 1363
ApaI	GGGCCC	1 cut(s)	1009
ApeKI	GCWGC	5 cut(s)	713, 776, 902, 1279, 1640
ApoI	RAATTY	7 cut(s)	407, 448, 462, 482, 574, 979, 1589
ArsI	GACNNNNNNTTYG	2 cut(s)	965, 997
Asp700I	GAANNNNTTC	1 cut(s)	482
AspA2I	CCTAGG	1 cut(s)	1555
AspLEI	GCGC	2 cut(s)	674, 884
AspS9I	GGNCC	6 cut(s)	347, 864, 1005, 1006, 1032, 1071
AsuC2I	CCSGG	5 cut(s)	1284, 1362, 1505, 1614, 1615
AsuHPI	GGTGA	8 cut(s)	23, 428, 1376, 1573, 1660, 1691, 1736, 1850
AsuII	TTCGAA	1 cut(s)	1572
AvaI	CYCGRG	2 cut(s)	629, 1613
AvaII	GGWCC	3 cut(s)	347, 1032, 1071
AvrII	CCTAGG	1 cut(s)	1555
BaeGI	GKGCMC	1 cut(s)	1009
BalI	TGGCCA	1 cut(s)	1297
BanII	GRGCYC	1 cut(s)	1009
BbsI	GAAGAC	3 cut(s)	608, 1386, 1851
Bbv12I	GWGCWC	1 cut(s)	1407
BbvCI	CCTCAGC	2 cut(s)	1001, 1014
BbvI	GCAGC	5 cut(s)	700, 763, 914, 1291, 1627
BccI	CCATC	5 cut(s)	42, 116, 467, 764, 1862
BceAI	ACGGC	2 cut(s)	244, 801
BciT130I	CCWGG	3 cut(s)	269, 1275, 1433
BclI	TGATCA	2 cut(s)	28, 1311
BcnI	CCSGG	5 cut(s)	1284, 1362, 1505, 1614, 1615
BcoDI	GTCTC	1 cut(s)	269
BfaI	CTAG	8 cut(s)	53, 357, 1248, 1409, 1457, 1556, 1863, 1887
BfoI	RGCGCY	1 cut(s)	675
BfuAI	ACCTGC	1 cut(s)	197
BisI	GCNGC	5 cut(s)	714, 777, 903, 1280, 1641
BlnI	CCTAGG	1 cut(s)	1555
BlpI	GCTNAGC	1 cut(s)	1263
BlsI	GCNGC	5 cut(s)	715, 778, 904, 1281, 1642
BmcAI	AGTACT	1 cut(s)	66
Bme1390I	CCNGG	8 cut(s)	269, 1275, 1284, 1362, 1433, 1505, 1614, 1615
Bme18I	GGWCC	3 cut(s)	347, 1032, 1071
BmeT110I	CYCGRG	2 cut(s)	629, 1613
BmgT120I	GGNCC	6 cut(s)	347, 864, 1005, 1006, 1032, 1071
BmiI	GGNNCC	6 cut(s)	865, 1007, 1008, 1033, 1072, 1553
BmrFI	CCNGG	8 cut(s)	269, 1275, 1284, 1362, 1433, 1505, 1614, 1615
BmrI	ACTGGG	1 cut(s)	869
BmsI	GCATC	3 cut(s)	29, 1719, 1728
BmuI	ACTGGG	1 cut(s)	869
BpiI	GAAGAC	3 cut(s)	608, 1386, 1851
Bpu10I	CCTNAGC	2 cut(s)	1001, 1014
Bpu1102I	GCTNAGC	1 cut(s)	1263
Bpu14I	TTCGAA	1 cut(s)	1572
BpuEI	CTTGAG	2 cut(s)	101, 1373
BpuMI	CCSGG	5 cut(s)	1284, 1362, 1505, 1614, 1615
Bsa29I	ATCGAT	1 cut(s)	279
BsaBI	GATNNNNATC	2 cut(s)	33, 1313
BsaHI	GRCGYC	1 cut(s)	814
BsaI	GGTCTC	1 cut(s)	269
BsaJI	CCNNGG	8 cut(s)	268, 1074, 1274, 1360, 1378, 1503, 1555, 1613
BsaWI	WCCGGW	1 cut(s)	1583
BsaXI	ACNNNNNCTCC	2 cut(s)	1769, 1799
Bsc4I	CCNNNNNNNGG	3 cut(s)	185, 1015, 1548
Bse1I	ACTGG	4 cut(s)	44, 164, 714, 864
Bse3DI	GCAATG	3 cut(s)	238, 750, 891
Bse8I	GATNNNNATC	2 cut(s)	33, 1313
BseBI	CCWGG	3 cut(s)	269, 1275, 1433
BseCI	ATCGAT	1 cut(s)	279
BseDI	CCNNGG	8 cut(s)	268, 1074, 1274, 1360, 1378, 1503, 1555, 1613
BseGI	GGATG	4 cut(s)	108, 478, 1758, 1879
BseJI	GATNNNNATC	2 cut(s)	33, 1313
BseLI	CCNNNNNNNGG	3 cut(s)	185, 1015, 1548
BseMI	GCAATG	3 cut(s)	238, 750, 891
BseMII	CTCAG	7 cut(s)	644, 783, 930, 992, 1005, 1668, 1890
BseNI	ACTGG	4 cut(s)	44, 164, 714, 864
BseRI	GAGGAG	3 cut(s)	104, 960, 1795
BseSI	GKGCMC	1 cut(s)	1009
BseXI	GCAGC	5 cut(s)	700, 763, 914, 1291, 1627
BseYI	CCCAGC	2 cut(s)	1009, 1594
Bsh1236I	CGCG	1 cut(s)	1631
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	6 cut(s)	651, 866, 1007, 1297, 1340, 1365
BshVI	ATCGAT	1 cut(s)	279
BsiEI	CGRYCG	1 cut(s)	99
BsiHKAI	GWGCWC	1 cut(s)	1407
BsiHKCI	CYCGRG	2 cut(s)	629, 1613
BsiSI	CCGG	6 cut(s)	644, 1283, 1362, 1505, 1584, 1614
BslFI	GGGAC	3 cut(s)	1486, 1720, 1847
BslI	CCNNNNNNNGG	3 cut(s)	185, 1015, 1548
BsmAI	GTCTC	1 cut(s)	269
BsmFI	GGGAC	3 cut(s)	1486, 1720, 1847
BsnI	GGCC	6 cut(s)	651, 866, 1007, 1297, 1340, 1365
Bso31I	GGTCTC	1 cut(s)	269
BsoBI	CYCGRG	2 cut(s)	629, 1613
Bsp119I	TTCGAA	1 cut(s)	1572
Bsp120I	GGGCCC	1 cut(s)	1005
Bsp1286I	GDGCHC	2 cut(s)	1009, 1407
Bsp143I	GATC	3 cut(s)	28, 91, 1311
Bsp1720I	GCTNAGC	1 cut(s)	1263
Bsp19I	CCATGG	1 cut(s)	1074
BspACI	CCGC	2 cut(s)	581, 1631
BspANI	GGCC	6 cut(s)	651, 866, 1007, 1297, 1340, 1365
BspCNI	CTCAG	7 cut(s)	645, 782, 931, 993, 1006, 1667, 1891
BspDI	ATCGAT	1 cut(s)	279
BspFNI	CGCG	1 cut(s)	1631
BspHI	TCATGA	2 cut(s)	327, 1219
BspLI	GGNNCC	6 cut(s)	865, 1007, 1008, 1033, 1072, 1553
BspMI	ACCTGC	1 cut(s)	197
BspPI	GGATC	1 cut(s)	86
BspT104I	TTCGAA	1 cut(s)	1572
BspTNI	GGTCTC	1 cut(s)	269
BsrDI	GCAATG	3 cut(s)	238, 750, 891
BsrI	ACTGG	4 cut(s)	44, 164, 714, 864
BssECI	CCNNGG	8 cut(s)	268, 1074, 1274, 1360, 1378, 1503, 1555, 1613
BssMI	GATC	3 cut(s)	28, 91, 1311
BssNI	GRCGYC	1 cut(s)	814
BssT1I	CCWWGG	2 cut(s)	1074, 1555
Bst2UI	CCWGG	3 cut(s)	269, 1275, 1433
Bst4CI	ACNGT	5 cut(s)	146, 188, 847, 1382, 1838
Bst6I	CTCTTC	8 cut(s)	91, 987, 1079, 1197, 1613, 1662, 1812, 1888
BstACI	GRCGYC	1 cut(s)	814
BstAPI	GCANNNNNTGC	1 cut(s)	1442
BstBI	TTCGAA	1 cut(s)	1572
BstC8I	GCNNGC	4 cut(s)	105, 510, 649, 718
BstDSI	CCRYGG	2 cut(s)	1074, 1378
BstEII	GGTNACC	1 cut(s)	1382
BstF5I	GGATG	4 cut(s)	108, 478, 1758, 1879
BstFNI	CGCG	1 cut(s)	1631
BstH2I	RGCGCY	1 cut(s)	675
BstHHI	GCGC	2 cut(s)	674, 884
BstKTI	GATC	3 cut(s)	31, 94, 1314
BstMAI	GTCTC	1 cut(s)	269
BstMBI	GATC	3 cut(s)	28, 91, 1311
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	5 cut(s)	49, 1442, 1637, 1738, 1767
BstNI	CCWGG	3 cut(s)	269, 1275, 1433
BstNSI	RCATGY	1 cut(s)	720
BstPI	GGTNACC	1 cut(s)	1382
BstSCI	CCNGG	8 cut(s)	267, 1273, 1282, 1360, 1431, 1503, 1612, 1613
BstSLI	GKGCMC	1 cut(s)	1009
BstUI	CGCG	1 cut(s)	1631
BstV1I	GCAGC	5 cut(s)	700, 763, 914, 1291, 1627
BstV2I	GAAGAC	3 cut(s)	608, 1386, 1851
BstX2I	RGATCY	1 cut(s)	91
BstXI	CCANNNNNNTGG	2 cut(s)	560, 597
BstYI	RGATCY	1 cut(s)	91
Bsu15I	ATCGAT	1 cut(s)	279
BsuRI	GGCC	6 cut(s)	651, 866, 1007, 1297, 1340, 1365
BsuTUI	ATCGAT	1 cut(s)	279
BtgI	CCRYGG	2 cut(s)	1074, 1378
BtsCI	GGATG	4 cut(s)	108, 478, 1758, 1879
BtsI	GCAGTG	1 cut(s)	1777
BtsIMutI	CAGTG	5 cut(s)	37, 151, 565, 707, 1777
BveI	ACCTGC	1 cut(s)	197
Cac8I	GCNNGC	4 cut(s)	105, 510, 649, 718
CaiI	CAGNNNCTG	1 cut(s)	776
CciI	TCATGA	2 cut(s)	327, 1219
CfoI	GCGC	2 cut(s)	674, 884
Cfr13I	GGNCC	6 cut(s)	347, 864, 1005, 1006, 1032, 1071
Cfr9I	CCCGGG	1 cut(s)	1613
ClaI	ATCGAT	1 cut(s)	279
CseI	GACGC	1 cut(s)	822
Csp6I	GTAC	5 cut(s)	65, 189, 238, 848, 1879
CviQI	GTAC	5 cut(s)	65, 189, 238, 848, 1879
DpnI	GATC	3 cut(s)	30, 93, 1313
DpnII	GATC	3 cut(s)	28, 91, 1311
DrdI	GACNNNNNNGTC	1 cut(s)	633
DseDI	GACNNNNNNGTC	1 cut(s)	633
EaeI	YGGCCR	1 cut(s)	1295
Eam1104I	CTCTTC	8 cut(s)	91, 987, 1079, 1197, 1613, 1662, 1812, 1888
EarI	CTCTTC	8 cut(s)	91, 987, 1079, 1197, 1613, 1662, 1812, 1888
Eco130I	CCWWGG	2 cut(s)	1074, 1555
Eco147I	AGGCCT	1 cut(s)	651
Eco24I	GRGCYC	1 cut(s)	1009
Eco31I	GGTCTC	1 cut(s)	269
Eco47I	GGWCC	3 cut(s)	347, 1032, 1071
Eco47III	AGCGCT	1 cut(s)	673
Eco57I	CTGAAG	5 cut(s)	72, 927, 989, 1754, 1763
Eco88I	CYCGRG	2 cut(s)	629, 1613
Eco91I	GGTNACC	1 cut(s)	1382
EcoO109I	RGGNCCY	4 cut(s)	347, 1005, 1006, 1032
EcoO65I	GGTNACC	1 cut(s)	1382
EcoRI	GAATTC	2 cut(s)	448, 482
EcoRII	CCWGG	3 cut(s)	267, 1273, 1431
EcoT14I	CCWWGG	2 cut(s)	1074, 1555
EcoT22I	ATGCAT	1 cut(s)	598
EcoT38I	GRGCYC	1 cut(s)	1009
ErhI	CCWWGG	2 cut(s)	1074, 1555
FaqI	GGGAC	3 cut(s)	1486, 1720, 1847
FauNDI	CATATG	1 cut(s)	25
FbaI	TGATCA	2 cut(s)	28, 1311
Fnu4HI	GCNGC	5 cut(s)	714, 777, 903, 1280, 1641
FokI	GGATG	4 cut(s)	95, 485, 1765, 1886
FriOI	GRGCYC	1 cut(s)	1009
Fsp4HI	GCNGC	5 cut(s)	714, 777, 903, 1280, 1641
FspBI	CTAG	8 cut(s)	53, 357, 1248, 1409, 1457, 1556, 1863, 1887
GlaI	GCGC	2 cut(s)	673, 883
GluI	GCNGC	5 cut(s)	714, 777, 903, 1280, 1641
GsaI	CCCAGC	2 cut(s)	1013, 1598
HaeII	RGCGCY	1 cut(s)	675
HaeIII	GGCC	6 cut(s)	651, 866, 1007, 1297, 1340, 1365
HapII	CCGG	6 cut(s)	644, 1283, 1362, 1505, 1584, 1614
HgaI	GACGC	1 cut(s)	822
HhaI	GCGC	2 cut(s)	674, 884
Hin1I	GRCGYC	1 cut(s)	814
Hin6I	GCGC	2 cut(s)	672, 882
HinP1I	GCGC	2 cut(s)	672, 882
HincII	GTYRAC	1 cut(s)	1462
HindII	GTYRAC	1 cut(s)	1462
HindIII	AAGCTT	2 cut(s)	322, 1240
HinfI	GANTC	1 cut(s)	627
HpaII	CCGG	6 cut(s)	644, 1283, 1362, 1505, 1584, 1614
HphI	GGTGA	8 cut(s)	23, 428, 1376, 1573, 1660, 1691, 1736, 1850
Hpy166II	GTNNAC	9 cut(s)	238, 364, 377, 391, 1462, 1651, 1674, 1807, 1879
Hpy188I	TCNGA	5 cut(s)	91, 96, 683, 969, 1900
Hpy188III	TCNNGA	4 cut(s)	328, 470, 928, 1220
Hpy8I	GTNNAC	9 cut(s)	238, 364, 377, 391, 1462, 1651, 1674, 1807, 1879
Hpy99I	CGWCG	4 cut(s)	230, 683, 816, 1714
HpyAV	CCTTC	7 cut(s)	338, 752, 964, 1134, 1143, 1696, 1715
HpyCH4III	ACNGT	5 cut(s)	146, 188, 847, 1382, 1838
HpyCH4IV	ACGT	3 cut(s)	75, 1370, 1401
HpyCH4V	TGCA	5 cut(s)	183, 596, 716, 720, 779
HpyF10VI	GCNNNNNNNGC	5 cut(s)	49, 1442, 1637, 1738, 1767
HpySE526I	ACGT	3 cut(s)	75, 1370, 1401
Hsp92I	GRCGYC	1 cut(s)	814
HspAI	GCGC	2 cut(s)	672, 882
Ksp22I	TGATCA	2 cut(s)	28, 1311
Kzo9I	GATC	3 cut(s)	28, 91, 1311
LmnI	GCTCC	4 cut(s)	181, 1176, 1410, 1551
Lsp1109I	GCAGC	5 cut(s)	700, 763, 914, 1291, 1627
LweI	GCATC	3 cut(s)	29, 1719, 1728
MaeI	CTAG	8 cut(s)	53, 357, 1248, 1409, 1457, 1556, 1863, 1887
MaeII	ACGT	3 cut(s)	75, 1370, 1401
MaeIII	GTNAC	4 cut(s)	838, 1155, 1371, 1382
MalI	GATC	3 cut(s)	30, 93, 1313
MboI	GATC	3 cut(s)	28, 91, 1311
MflI	RGATCY	1 cut(s)	91
MhlI	GDGCHC	2 cut(s)	1009, 1407
MlsI	TGGCCA	1 cut(s)	1297
MluNI	TGGCCA	1 cut(s)	1297
MlyI	GAGTC	1 cut(s)	621
MmeI	TCCRAC	7 cut(s)	119, 162, 661, 687, 969, 1422, 1633
Mox20I	TGGCCA	1 cut(s)	1297
Mph1103I	ATGCAT	1 cut(s)	598
MroXI	GAANNNNTTC	1 cut(s)	482
MscI	TGGCCA	1 cut(s)	1297
MseI	TTAA	5 cut(s)	251, 728, 1092, 1823, 1923
MslI	CAYNNNNRTG	3 cut(s)	293, 1079, 1257
Msp20I	TGGCCA	1 cut(s)	1297
MspA1I	CMGCKG	2 cut(s)	905, 1013
MspI	CCGG	6 cut(s)	644, 1283, 1362, 1505, 1584, 1614
MspR9I	CCNGG	8 cut(s)	269, 1275, 1284, 1362, 1433, 1505, 1614, 1615
MvaI	CCWGG	3 cut(s)	269, 1275, 1433
MvnI	CGCG	1 cut(s)	1631
MwoI	GCNNNNNNNGC	5 cut(s)	49, 1442, 1637, 1738, 1767
NciI	CCSGG	5 cut(s)	1284, 1362, 1505, 1614, 1615
NcoI	CCATGG	1 cut(s)	1074
NdeI	CATATG	1 cut(s)	25
NdeII	GATC	3 cut(s)	28, 91, 1311
NlaIV	GGNNCC	6 cut(s)	865, 1007, 1008, 1033, 1072, 1553
NmuCI	GTSAC	3 cut(s)	838, 1155, 1382
NsiI	ATGCAT	1 cut(s)	598
NspI	RCATGY	1 cut(s)	720
NspV	TTCGAA	1 cut(s)	1572
PaeI	GCATGC	1 cut(s)	720
PaeR7I	CTCGAG	1 cut(s)	629
PagI	TCATGA	2 cut(s)	327, 1219
PceI	AGGCCT	1 cut(s)	651
PdmI	GAANNNNTTC	1 cut(s)	482
PkrI	GCNGC	5 cut(s)	715, 778, 904, 1281, 1642
PleI	GAGTC	1 cut(s)	621
PpsI	GAGTC	1 cut(s)	621
PpuMI	RGGWCCY	2 cut(s)	347, 1032
Psp1406I	AACGTT	1 cut(s)	1370
Psp5II	RGGWCCY	2 cut(s)	347, 1032
Psp6I	CCWGG	3 cut(s)	267, 1273, 1431
PspEI	GGTNACC	1 cut(s)	1382
PspFI	CCCAGC	2 cut(s)	1009, 1594
PspGI	CCWGG	3 cut(s)	267, 1273, 1431
PspN4I	GGNNCC	6 cut(s)	865, 1007, 1008, 1033, 1072, 1553
PspOMI	GGGCCC	1 cut(s)	1005
PspPI	GGNCC	6 cut(s)	347, 864, 1005, 1006, 1032, 1071
PspPPI	RGGWCCY	2 cut(s)	347, 1032
PspXI	VCTCGAGB	1 cut(s)	629
PstNI	CAGNNNCTG	1 cut(s)	776
PsuI	RGATCY	1 cut(s)	91
PvuII	CAGCTG	2 cut(s)	905, 1013
RsaI	GTAC	5 cut(s)	66, 190, 239, 849, 1880
RsaNI	GTAC	5 cut(s)	65, 189, 238, 848, 1879
RseI	CAYNNNNRTG	3 cut(s)	293, 1079, 1257
SaqAI	TTAA	5 cut(s)	251, 728, 1092, 1823, 1923
SatI	GCNGC	5 cut(s)	714, 777, 903, 1280, 1641
Sau3AI	GATC	3 cut(s)	28, 91, 1311
Sau96I	GGNCC	6 cut(s)	347, 864, 1005, 1006, 1032, 1071
ScaI	AGTACT	1 cut(s)	66
SchI	GAGTC	1 cut(s)	621
ScrFI	CCNGG	8 cut(s)	269, 1275, 1284, 1362, 1433, 1505, 1614, 1615
SduI	GDGCHC	2 cut(s)	1009, 1407
SfaNI	GCATC	3 cut(s)	29, 1719, 1728
Sfr274I	CTCGAG	1 cut(s)	629
SfuI	TTCGAA	1 cut(s)	1572
SinI	GGWCC	3 cut(s)	347, 1032, 1071
SlaI	CTCGAG	1 cut(s)	629
SmaI	CCCGGG	1 cut(s)	1615
SmiMI	CAYNNNNRTG	3 cut(s)	293, 1079, 1257
SmlI	CTYRAG	3 cut(s)	116, 629, 1388
SmoI	CTYRAG	3 cut(s)	116, 629, 1388
SphI	GCATGC	1 cut(s)	720
SseBI	AGGCCT	1 cut(s)	651
SsiI	CCGC	2 cut(s)	581, 1631
SspMI	CTAG	8 cut(s)	53, 357, 1248, 1409, 1457, 1556, 1863, 1887
StuI	AGGCCT	1 cut(s)	651
StyD4I	CCNGG	8 cut(s)	267, 1273, 1282, 1360, 1431, 1503, 1612, 1613
StyI	CCWWGG	2 cut(s)	1074, 1555
TaaI	ACNGT	5 cut(s)	146, 188, 847, 1382, 1838
TaiI	ACGT	3 cut(s)	78, 1373, 1404
TaqI	TCGA	4 cut(s)	279, 630, 894, 1572
TaqII	GACCGA	2 cut(s)	113, 1556
TatI	WGTACW	3 cut(s)	64, 847, 1878
Tru1I	TTAA	5 cut(s)	251, 728, 1092, 1823, 1923
Tru9I	TTAA	5 cut(s)	251, 728, 1092, 1823, 1923
TscAI	CASTG	5 cut(s)	44, 151, 565, 714, 1777
TseFI	GTSAC	3 cut(s)	838, 1155, 1382
TseI	GCWGC	5 cut(s)	713, 776, 902, 1279, 1640
Tsp45I	GTSAC	3 cut(s)	838, 1155, 1382
TspGWI	ACGGA	1 cut(s)	1731
TspMI	CCCGGG	1 cut(s)	1613
TspRI	CASTG	5 cut(s)	44, 151, 565, 714, 1777
VpaK11BI	GGWCC	3 cut(s)	347, 1032, 1071
XapI	RAATTY	7 cut(s)	407, 448, 462, 482, 574, 979, 1589
XceI	RCATGY	1 cut(s)	720
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1240
XhoI	CTCGAG	1 cut(s)	629
XmaI	CCCGGG	1 cut(s)	1613
XmaJI	CCTAGG	1 cut(s)	1555
XmnI	GAANNNNTTC	1 cut(s)	482
XspI	CTAG	8 cut(s)	53, 357, 1248, 1409, 1457, 1556, 1863, 1887
ZrmI	AGTACT	1 cut(s)	66
Zsp2I	ATGCAT	1 cut(s)	598

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.