Rroxscaffold_7G00162890

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002722
Physical Location & Seq
Forward (+)
5263061 .. 5263597
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162890.1

Sequence Viewer

Length: 537 bp
ATGGCTCGTAACAAAGCAGCAGCAGCACACAATCAATTGCTGCTATTAATAGAAATATTTCTGTTAGCAATAGTATCATCATGTGTTATCTCATCTTCTAGTGCTGCTAGTGAGGCTGAAACGCTCATCACCTTCAAGAATTCTCTATCCGATACCTCGGCACTAGATAACTGGAATGCTTCTACAAAACCCTGCAACGGGTCCCAGAGCCATTGGACGGGTGTGCAGTGTAACAACGGGAGCGTATCTGGGCTGCAGCTGGAGAACATGAGCCTAACTGGCCTGATTGACCTTGACACGCTCACACAGTTGCCGGCCCTCAAGGCCATAAGCTTCATGAACAACGGCTTTGGAGGTCCACTGCCCCATGTGAAGAGACTTTCACTCCAAGGTCTCTACTTATCACAGAACCAATTCTCTGGTGATATCCCGGATGACGCTTTCAGTGGCATGGGTGATACCTTGAAGAAGCTTTACTTGGCCAAAAATGGATTTATGGGGAAGATACCGAGTTCTTTGGTTGATTTGCCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

178

Amino Acids

18.89

Weight (kDa)

5.83

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 78 5.7e-09 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 1 cut(s) 139
AfiI CCNNNNNNNGG 3 cut(s) 197, 198, 217
AgsI TTSAA 2 cut(s) 136, 466
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 3 cut(s) 259, 333, 472
AluI AGCT 3 cut(s) 259, 333, 472
Alw26I GTCTC 2 cut(s) 370, 398
AoxI GGCC 4 cut(s) 280, 315, 324, 480
ApeKI GCWGC 7 cut(s) 17, 20, 23, 40, 104, 253, 256
ApoI RAATTY 1 cut(s) 139
AseI ATTAAT 1 cut(s) 47
Asp700I GAANNNNTTC 2 cut(s) 57, 413
AspS9I GGNCC 3 cut(s) 201, 316, 356
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 3 cut(s) 121, 434, 467
AvaII GGWCC 2 cut(s) 201, 356
BalI TGGCCA 1 cut(s) 482
BarI GAAGNNNNNNTAC 2 cut(s) 458, 490
BbvI GCAGC 7 cut(s) 27, 29, 32, 35, 91, 240, 268
BceAI ACGGC 1 cut(s) 361
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 2 cut(s) 370, 398
BfaI CTAG 3 cut(s) 99, 108, 164
BfmI CTRYAG 1 cut(s) 254
BglI GCCNNNNNGGC 2 cut(s) 279, 323
BisI GCNGC 7 cut(s) 18, 21, 24, 41, 105, 254, 257
BlsI GCNGC 7 cut(s) 19, 22, 25, 42, 106, 255, 258
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 2 cut(s) 201, 356
BmgT120I GGNCC 3 cut(s) 201, 316, 356
BmiI GGNNCC 2 cut(s) 202, 203
BmrFI CCNGG 1 cut(s) 431
BpmI CTGGAG 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 156, 388
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 198, 217
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 2 cut(s) 176, 283
BseDI CCNNGG 2 cut(s) 156, 388
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 3 cut(s) 197, 198, 217
BseNI ACTGG 2 cut(s) 176, 283
BseXI GCAGC 7 cut(s) 27, 29, 32, 35, 91, 240, 268
BsgI GTGCAG 1 cut(s) 245
BshFI GGCC 4 cut(s) 282, 317, 326, 482
BsiSI CCGG 2 cut(s) 314, 431
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 3 cut(s) 197, 198, 217
BsmAI GTCTC 2 cut(s) 370, 398
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 4 cut(s) 282, 317, 326, 482
Bso31I GGTCTC 1 cut(s) 398
BspANI GGCC 4 cut(s) 282, 317, 326, 482
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 2 cut(s) 202, 203
BspMAI CTGCAG 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 398
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 2 cut(s) 176, 283
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 156, 388
BssT1I CCWWGG 1 cut(s) 388
Bst4CI ACNGT 1 cut(s) 309
Bst6I CTCTTC 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 315
BstF5I GGATG 1 cut(s) 439
BstMAI GTCTC 2 cut(s) 370, 398
BstMWI GCNNNNNNNGC 4 cut(s) 23, 113, 279, 323
BstSCI CCNGG 1 cut(s) 429
BstSFI CTRYAG 1 cut(s) 254
BstV1I GCAGC 7 cut(s) 27, 29, 32, 35, 91, 240, 268
BstXI CCANNNNNNTGG 1 cut(s) 419
BsuRI GGCC 4 cut(s) 282, 317, 326, 482
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 2 cut(s) 233, 359
BtsIMutI CAGTG 3 cut(s) 233, 359, 451
Cac8I GCNNGC 1 cut(s) 315
CciI TCATGA 1 cut(s) 336
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 3 cut(s) 201, 316, 356
CseI GACGC 1 cut(s) 446
CviAII CATG 5 cut(s) 81, 268, 337, 368, 451
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 398
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 201, 356
EcoO109I RGGNCCY 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 139
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 5 cut(s) 84, 271, 340, 371, 454
FaiI YATR 7 cut(s) 82, 269, 329, 338, 369, 452, 497
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 1 cut(s) 187
FatI CATG 5 cut(s) 80, 267, 336, 367, 450
Fnu4HI GCNGC 7 cut(s) 18, 21, 24, 41, 105, 254, 257
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 7 cut(s) 18, 21, 24, 41, 105, 254, 257
FspBI CTAG 3 cut(s) 99, 108, 164
GluI GCNGC 7 cut(s) 18, 21, 24, 41, 105, 254, 257
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 4 cut(s) 282, 317, 326, 482
HapII CCGG 2 cut(s) 314, 431
HgaI GACGC 1 cut(s) 446
Hin1II CATG 5 cut(s) 84, 271, 340, 371, 454
HindIII AAGCTT 2 cut(s) 331, 470
HpaII CCGG 2 cut(s) 314, 431
HphI GGTGA 3 cut(s) 121, 434, 467
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 2 cut(s) 136, 337
Hpy8I GTNNAC 1 cut(s) 359
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4V TGCA 3 cut(s) 195, 226, 256
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 113, 279, 323
Hsp92II CATG 5 cut(s) 84, 271, 340, 371, 454
KflI GGGWCCC 1 cut(s) 201
KroI GCCGGC 1 cut(s) 313
KroNI GCCGGC 1 cut(s) 315
LmnI GCTCC 1 cut(s) 240
Lsp1109I GCAGC 7 cut(s) 27, 29, 32, 35, 91, 240, 268
MaeI CTAG 3 cut(s) 99, 108, 164
MaeIII GTNAC 2 cut(s) 8, 230
MboII GAAGA 4 cut(s) 87, 385, 478, 514
MfeI CAATTG 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 4 cut(s) 35, 139, 413, 532
MluNI TGGCCA 1 cut(s) 482
MnlI CCTC 4 cut(s) 106, 166, 329, 347
Mox20I TGGCCA 1 cut(s) 482
MroNI GCCGGC 1 cut(s) 313
MroXI GAANNNNTTC 2 cut(s) 57, 413
MscI TGGCCA 1 cut(s) 482
MseI TTAA 2 cut(s) 47, 535
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 2 cut(s) 314, 431
MspR9I CCNGG 1 cut(s) 431
MunI CAATTG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 181
MwoI GCNNNNNNNGC 4 cut(s) 23, 113, 279, 323
NaeI GCCGGC 1 cut(s) 315
NciI CCSGG 1 cut(s) 431
NgoMIV GCCGGC 1 cut(s) 313
NlaIII CATG 5 cut(s) 84, 271, 340, 371, 454
NlaIV GGNNCC 2 cut(s) 202, 203
NmeAIII GCCGAG 1 cut(s) 137
PagI TCATGA 1 cut(s) 336
PctI GAATGC 1 cut(s) 181
PdiI GCCGGC 1 cut(s) 315
PdmI GAANNNNTTC 2 cut(s) 57, 413
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 7 cut(s) 19, 22, 25, 42, 106, 255, 258
PpuMI RGGWCCY 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 202, 203
PspPI GGNCC 3 cut(s) 201, 316, 356
PspPPI RGGWCCY 1 cut(s) 201
PstI CTGCAG 1 cut(s) 258
PvuII CAGCTG 1 cut(s) 259
SaqAI TTAA 2 cut(s) 47, 535
SatI GCNGC 7 cut(s) 18, 21, 24, 41, 105, 254, 257
Sau96I GGNCC 3 cut(s) 201, 316, 356
ScrFI CCNGG 1 cut(s) 431
SetI ASST 9 cut(s) 134, 158, 261, 294, 335, 358, 394, 464, 474
SfcI CTRYAG 1 cut(s) 254
SfiI GGCCNNNNNGGCC 1 cut(s) 323
SinI GGWCC 2 cut(s) 201, 356
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 4 cut(s) 35, 139, 413, 532
SspI AATATT 1 cut(s) 57
SspMI CTAG 3 cut(s) 99, 108, 164
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 1 cut(s) 309
TasI AATT 4 cut(s) 35, 139, 413, 532
Tru1I TTAA 2 cut(s) 47, 535
Tru9I TTAA 2 cut(s) 47, 535
TscAI CASTG 3 cut(s) 233, 366, 451
TseI GCWGC 7 cut(s) 17, 20, 23, 40, 104, 253, 256
TspDTI ATGAA 2 cut(s) 325, 353
TspRI CASTG 3 cut(s) 233, 366, 451
VpaK11BI GGWCC 2 cut(s) 201, 356
VspI ATTAAT 1 cut(s) 47
XapI RAATTY 1 cut(s) 139
XmnI GAANNNNTTC 2 cut(s) 57, 413
XspI CTAG 3 cut(s) 99, 108, 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.