RchiOBHm_Chr2g0149011
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002722
Physical Location & Seq
Forward (+)
66870688 .. 66873499
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51844

Sequence Viewer

Length: 2037 bp
ATGGGCGCGCATATGGCTATGGCTAGGCTTATGCGCGCACCAAACCCATCATTCCTGCTCGCTCTCATTGTAGCTACGCTTTCTTTTATGAATATGTTGTCTTTTGGTTTGCCGGAGACCACGTCCGACGCCCTGTTAAAATTCAAAAGTTCTTTGGGGAACAGTTCCGAAGCTCTTAGGAATTGGGGTAGTTCTCCGAACCCATGCAATGGTAACAGGGGGAATTGGGTCGGTGTGCTTTGCTTTCAAGGGAATGTCAGGGGCTTGAAACTCGAAGGCATGGGGTTACAGGGAACGTTAGATATGGAACCTCTCGCGTCGTTGCCATATTTGAAGACTTTAAGCTTCGCGAACAATTCATTCATAGGTCCGTTGCCTAATCTCACTAGACTAAAGAGATTAGGGTCGTTATATTTGTCAGATAATCATTTGTCTGGGGATATTCCTGATAATGCATTTGAGAATTTGACTTTCTTGAAGAAACTGTATTTGGGAAATAATGAGTTCTCAGGCAAAATCCCTTCATCTCTTGCCAAATTGCCAAACCTTTTCGACGTGGGGCTTGAAAATAACTATTTTAGTGGCGAAATCCCTGATTTTAAAAGTGGGGACTCAGAGAAATTATGGAGGTTGTATTTGGCTAACAATGAGTTGGAGGGAGAAATCCCAAAAAGCCTGAGAACGCAGGAGCCTAGCAGTTTTGCAGGAAATGAAAACCTATGTGGTCCGCCTATAGAAGCATGTAGCCCCTCTCCTGCTCCTGCTCCTACATCATCTCCATCGCCATCTTCGCCTCCATGTGGGGGATCCGGGGAACCATGCAGCCCTTCCGACCCTCCTACGAAGTCTTCATCTCTTGGTCTAAAAATAGCCTTGGGTATAGTGAGTATTCTGCTCCTATTAGTTATCATTGCTGTACTTTTGATGTTCCTCAGAAAGAGAAAGCAACAACCGGAATTAGACCAGGCGGAGTTGTTGGATGAATCCTCCAAGTACACAGCAGCAACCGCGAGCCAGATGGATGCCAAGTCTGTAGAAAATAATAATGTGAACAATCCACCAAGGAGGGGTGAGCAAGCAGGAAAGCTGTCGTTTGTGAGGGATGACAGGGACAGGTTTGATTTGCATGACCTGCTAAGAGCCTCAGCCGAAATATTGGGGAGTGGGAACTTTGGGGCTTCGTACAAGGCTTTGATACTAACTGATGCTGTAGTGGTCAAGAGGTATAAGCAGATGAACAATGTGGGAAGAGAGGAGTTCCATGACCACATGAGAAGGCTTGGGAGATTGACACACCAAAACTTGTTGCCAATCGTGGCCTATTATTATAGAAGGGAAGAGAAGCTCTTGGTTTCTGACTTTGTGGAGAACGGTAGCTTGGCTTCTCATCTTCATGGCAATCACAATCTGCATAAGCCTGGACTTGACTGGCCTACCCGTTTGAGAATAATCAAGGGAATAGGAAGGGGCCTGACATACCTCTACAACACACTCCCAAGCTTGGTTGTACCTCATGGTCATCTGAAATCCTCCAATGTGCTTCTGGACGAAAACATGGAGCCTCTACTGAACGACTATGCTCTACTCCCCGTGATCAACATGGAACAAGCCCAACACCTCATGATGGCTTACAAGTCACCCGAATACGCGCGGCACCACCGGATAACAAAGAAGACCGACGTGTGGTGCTTCGGGATAATCATGTTGGAGGTGTTGACAGGCAAGTTCCCAGAAAACTATCTAAAGCAAAGTTATGACAGTAAGGCGGATTTGGCGAGCTGGGTGAACGGAATGATCAAGGAGAAGAAGACTAGTGAGGTGTTTGATGGAGAAATGGGGAATGTCGGTGACAGCAAAGGTGAACTTCTAAAGCTGTTGAAGATTGGAGTGAGTTGTTGTGAGGAGGATGTGGAGAGAAGGCTGGACTTGCATGAAGTTGTTGCAAAGATTGAGGAGTTGAATGAGGGAGAAAGTGATGGGGAATACCGCTCAAGTGTTTCAAGTGAAGAAGATGATTATACTTCTCAGGCTGTGTGA

Protein Analysis

678

Amino Acids

74.93

Weight (kDa)

5.95

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 81 7.2e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 133 - 192 5.2e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 384 - 649 1.3e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 384 - 648 1.1e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1140
AccB1I GGYRCC 1 cut(s) 1653
AccB7I CCANNNNNTGG 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 1989
AccII CGCG 7 cut(s) 8, 36, 317, 350, 1010, 1649, 1651
AciI CCGC 6 cut(s) 728, 968, 1008, 1651, 1766, 1987
AclI AACGTT 1 cut(s) 296
AclWI GGATC 2 cut(s) 801, 814
AcsI RAATTY 2 cut(s) 140, 463
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 4 cut(s) 918, 995, 1184, 1509
AfiI CCNNNNNNNGG 7 cut(s) 209, 800, 803, 1067, 1501, 1624, 1683
AflIII ACRYGT 1 cut(s) 1680
AgsI TTSAA 9 cut(s) 145, 248, 268, 334, 478, 566, 1879, 1960, 2001
AhlI ACTAGT 1 cut(s) 1811
AjiI CACGTC 3 cut(s) 123, 556, 1681
AjnI CCWGG 2 cut(s) 963, 1417
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 1361, 1393
AluBI AGCT 9 cut(s) 74, 173, 345, 1087, 1345, 1377, 1500, 1779, 1873
AluI AGCT 9 cut(s) 74, 173, 345, 1087, 1345, 1377, 1500, 1779, 1873
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 2 cut(s) 801, 814
AoxI GGCC 3 cut(s) 1317, 1430, 1468
ApeKI GCWGC 2 cut(s) 822, 1001
ApoI RAATTY 2 cut(s) 140, 463
AspLEI GCGC 5 cut(s) 8, 10, 36, 38, 1651
AspS9I GGNCC 3 cut(s) 368, 725, 1468
AsuC2I CCSGG 1 cut(s) 811
AsuHPI GGTGA 5 cut(s) 1082, 1629, 1795, 1859, 1871
AvaII GGWCC 2 cut(s) 368, 725
BamHI GGATCC 1 cut(s) 806
BanI GGYRCC 1 cut(s) 1653
BarI GAAGNNNNNNTAC 2 cut(s) 1366, 1398
BbsI GAAGAC 4 cut(s) 341, 840, 1679, 1814
BbvCI CCTCAGC 1 cut(s) 1144
BbvI GCAGC 2 cut(s) 834, 1013
BccI CCATC 7 cut(s) 55, 787, 793, 1012, 1618, 1820, 1970
BciT130I CCWGG 2 cut(s) 965, 1419
BclI TGATCA 2 cut(s) 1593, 1794
BcnI CCSGG 1 cut(s) 811
BcoDI GTCTC 1 cut(s) 110
BcuI ACTAGT 1 cut(s) 1811
BfaI CTAG 4 cut(s) 24, 387, 693, 1812
BfmI CTRYAG 3 cut(s) 732, 1032, 1209
BfuAI ACCTGC 1 cut(s) 1140
BisI GCNGC 3 cut(s) 823, 1002, 1652
BlsI GCNGC 3 cut(s) 824, 1003, 1653
Bme1390I CCNGG 3 cut(s) 811, 965, 1419
Bme18I GGWCC 2 cut(s) 368, 725
BmgBI CACGTC 3 cut(s) 123, 556, 1681
BmgT120I GGNCC 3 cut(s) 368, 725, 1468
BmiI GGNNCC 7 cut(s) 309, 690, 808, 816, 1469, 1560, 1655
BmrFI CCNGG 3 cut(s) 811, 965, 1419
BmsI GCATC 2 cut(s) 1012, 1195
BpiI GAAGAC 4 cut(s) 341, 840, 1679, 1814
Bpu10I CCTNAGC 1 cut(s) 1144
BpuEI CTTGAG 1 cut(s) 1975
BpuMI CCSGG 1 cut(s) 811
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 3 cut(s) 810, 873, 1061
BsaWI WCCGGW 2 cut(s) 952, 1659
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 760, 790
Bsc4I CCNNNNNNNGG 7 cut(s) 209, 800, 803, 1067, 1501, 1624, 1683
Bse1I ACTGG 1 cut(s) 1433
Bse3DI GCAATG 2 cut(s) 214, 909
BseBI CCWGG 2 cut(s) 965, 1419
BseDI CCNNGG 3 cut(s) 810, 873, 1061
BseGI GGATG 4 cut(s) 985, 1027, 1108, 1912
BseLI CCNNNNNNNGG 7 cut(s) 209, 800, 803, 1067, 1501, 1624, 1683
BseMI GCAATG 2 cut(s) 214, 909
BseMII CTCAG 5 cut(s) 522, 627, 668, 946, 1158
BseNI ACTGG 1 cut(s) 1433
BsePI GCGCGC 2 cut(s) 6, 34
BseRI GAGGAG 3 cut(s) 1268, 1916, 1967
BseXI GCAGC 2 cut(s) 834, 1013
BseYI CCCAGC 1 cut(s) 1779
Bsh1236I CGCG 7 cut(s) 8, 36, 317, 350, 1010, 1649, 1651
BshFI GGCC 3 cut(s) 1319, 1432, 1470
BshNI GGYRCC 1 cut(s) 1653
BsiSI CCGG 4 cut(s) 113, 810, 953, 1660
BslFI GGGAC 2 cut(s) 623, 1124
BslI CCNNNNNNNGG 7 cut(s) 209, 800, 803, 1067, 1501, 1624, 1683
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 2 cut(s) 623, 1124
BsnI GGCC 3 cut(s) 1319, 1432, 1470
Bso31I GGTCTC 1 cut(s) 110
Bsp143I GATC 3 cut(s) 806, 1593, 1794
Bsp68I TCGCGA 1 cut(s) 350
BspACI CCGC 6 cut(s) 728, 968, 1008, 1651, 1766, 1987
BspANI GGCC 3 cut(s) 1319, 1432, 1470
BspCNI CTCAG 5 cut(s) 521, 626, 669, 945, 1157
BspFNI CGCG 7 cut(s) 8, 36, 317, 350, 1010, 1649, 1651
BspHI TCATGA 1 cut(s) 1620
BspLI GGNNCC 7 cut(s) 309, 690, 808, 816, 1469, 1560, 1655
BspMI ACCTGC 1 cut(s) 1140
BspPI GGATC 2 cut(s) 801, 814
BspT107I GGYRCC 1 cut(s) 1653
BspTNI GGTCTC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 1989
BsrDI GCAATG 2 cut(s) 214, 909
BsrI ACTGG 1 cut(s) 1433
BssECI CCNNGG 3 cut(s) 810, 873, 1061
BssHII GCGCGC 2 cut(s) 6, 34
BssMI GATC 3 cut(s) 806, 1593, 1794
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 2 cut(s) 873, 1061
Bst2UI CCWGG 2 cut(s) 965, 1419
Bst4CI ACNGT 4 cut(s) 164, 486, 1373, 1760
Bst6I CTCTTC 2 cut(s) 1243, 1332
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 1132
BstC8I GCNNGC 6 cut(s) 8, 36, 60, 1012, 1077, 1777
BstDEI CTNAG 8 cut(s) 176, 508, 613, 677, 932, 1136, 1144, 2025
BstF5I GGATG 4 cut(s) 985, 1027, 1108, 1912
BstFNI CGCG 7 cut(s) 8, 36, 317, 350, 1010, 1649, 1651
BstHHI GCGC 5 cut(s) 8, 10, 36, 38, 1651
BstKTI GATC 3 cut(s) 809, 1596, 1797
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 3 cut(s) 806, 1593, 1794
BstMWI GCNNNNNNNGC 6 cut(s) 14, 790, 1007, 1132, 1772, 1927
BstNI CCWGG 2 cut(s) 965, 1419
BstNSI RCATGY 1 cut(s) 744
BstSCI CCNGG 3 cut(s) 809, 963, 1417
BstSFI CTRYAG 3 cut(s) 732, 1032, 1209
BstUI CGCG 7 cut(s) 8, 36, 317, 350, 1010, 1649, 1651
BstV1I GCAGC 2 cut(s) 834, 1013
BstV2I GAAGAC 4 cut(s) 341, 840, 1679, 1814
BstX2I RGATCY 1 cut(s) 806
BstYI RGATCY 1 cut(s) 806
BsuRI GGCC 3 cut(s) 1319, 1432, 1470
BtgZI GCGATG 1 cut(s) 765
BtrI CACGTC 3 cut(s) 123, 556, 1681
BtsCI GGATG 4 cut(s) 985, 1027, 1108, 1912
BtuMI TCGCGA 1 cut(s) 350
BveI ACCTGC 1 cut(s) 1140
Cac8I GCNNGC 6 cut(s) 8, 36, 60, 1012, 1077, 1777
CciI TCATGA 1 cut(s) 1620
CfoI GCGC 5 cut(s) 8, 10, 36, 38, 1651
Cfr13I GGNCC 3 cut(s) 368, 725, 1468
CseI GACGC 2 cut(s) 137, 306
Csp6I GTAC 4 cut(s) 917, 994, 1183, 1508
CviQI GTAC 4 cut(s) 917, 994, 1183, 1508
DdeI CTNAG 8 cut(s) 176, 508, 613, 677, 932, 1136, 1144, 2025
DpnI GATC 3 cut(s) 808, 1595, 1796
DpnII GATC 3 cut(s) 806, 1593, 1794
DraI TTTAAA 1 cut(s) 601
Eam1104I CTCTTC 2 cut(s) 1243, 1332
EarI CTCTTC 2 cut(s) 1243, 1332
EciI GGCGGA 3 cut(s) 717, 983, 1781
Eco130I CCWWGG 2 cut(s) 873, 1061
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 2 cut(s) 368, 725
EcoO109I RGGNCCY 1 cut(s) 1468
EcoRII CCWGG 2 cut(s) 963, 1417
EcoT14I CCWWGG 2 cut(s) 873, 1061
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 873, 1061
FalI AAGNNNNNCTT 2 cut(s) 1848, 1880
FaqI GGGAC 2 cut(s) 623, 1124
FauNDI CATATG 1 cut(s) 12
FbaI TGATCA 2 cut(s) 1593, 1794
Fnu4HI GCNGC 3 cut(s) 823, 1002, 1652
FokI GGATG 4 cut(s) 992, 1034, 1115, 1919
Fsp4HI GCNGC 3 cut(s) 823, 1002, 1652
FspBI CTAG 4 cut(s) 24, 387, 693, 1812
GlaI GCGC 5 cut(s) 7, 9, 35, 37, 1650
GluI GCNGC 3 cut(s) 823, 1002, 1652
GsaI CCCAGC 1 cut(s) 1783
HaeIII GGCC 3 cut(s) 1319, 1432, 1470
HapII CCGG 4 cut(s) 113, 810, 953, 1660
HgaI GACGC 2 cut(s) 137, 306
HhaI GCGC 5 cut(s) 8, 10, 36, 38, 1651
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 5 cut(s) 6, 8, 34, 36, 1649
HinP1I GCGC 5 cut(s) 6, 8, 34, 36, 1649
HincII GTYRAC 1 cut(s) 1716
HindII GTYRAC 1 cut(s) 1716
HindIII AAGCTT 2 cut(s) 343, 1498
HinfI GANTC 2 cut(s) 611, 983
HpaII CCGG 4 cut(s) 113, 810, 953, 1660
HphI GGTGA 5 cut(s) 1082, 1629, 1795, 1859, 1871
Hpy166II GTNNAC 5 cut(s) 996, 1051, 1716, 1786, 1862
Hpy188I TCNGA 9 cut(s) 127, 169, 198, 421, 616, 832, 935, 1357, 1524
Hpy188III TCNNGA 7 cut(s) 349, 446, 475, 1219, 1544, 1621, 1693
Hpy8I GTNNAC 5 cut(s) 996, 1051, 1716, 1786, 1862
Hpy99I CGWCG 4 cut(s) 131, 322, 557, 1682
HpyAV CCTTC 7 cut(s) 269, 531, 837, 1269, 1326, 1458, 1911
HpyCH4III ACNGT 4 cut(s) 164, 486, 1373, 1760
HpyCH4IV ACGT 4 cut(s) 122, 296, 555, 1680
HpyCH4V TGCA 8 cut(s) 207, 455, 704, 822, 1126, 1411, 1930, 1943
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 790, 1007, 1132, 1772, 1927
HpyF3I CTNAG 8 cut(s) 176, 508, 613, 677, 932, 1136, 1144, 2025
HpySE526I ACGT 4 cut(s) 122, 296, 555, 1680
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 5 cut(s) 6, 8, 34, 36, 1649
Ksp22I TGATCA 2 cut(s) 1593, 1794
Kzo9I GATC 3 cut(s) 806, 1593, 1794
LmnI GCTCC 5 cut(s) 688, 763, 769, 900, 1558
Lsp1109I GCAGC 2 cut(s) 834, 1013
LweI GCATC 2 cut(s) 1012, 1195
MaeI CTAG 4 cut(s) 24, 387, 693, 1812
MaeII ACGT 4 cut(s) 122, 296, 555, 1680
MaeIII GTNAC 4 cut(s) 212, 285, 1635, 1847
MalI GATC 3 cut(s) 808, 1595, 1796
MbiI CCGCTC 1 cut(s) 1989
MboI GATC 3 cut(s) 806, 1593, 1794
MflI RGATCY 1 cut(s) 806
MluCI AATT 8 cut(s) 140, 181, 223, 355, 463, 536, 620, 956
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 5 cut(s) 150, 633, 855, 957, 1686
Mph1103I ATGCAT 1 cut(s) 457
MseI TTAA 3 cut(s) 137, 341, 600
MslI CAYNNNNRTG 1 cut(s) 1392
MspI CCGG 4 cut(s) 113, 810, 953, 1660
MspR9I CCNGG 3 cut(s) 811, 965, 1419
MvaI CCWGG 2 cut(s) 965, 1419
MvnI CGCG 7 cut(s) 8, 36, 317, 350, 1010, 1649, 1651
MwoI GCNNNNNNNGC 6 cut(s) 14, 790, 1007, 1132, 1772, 1927
NciI CCSGG 1 cut(s) 811
NdeI CATATG 1 cut(s) 12
NdeII GATC 3 cut(s) 806, 1593, 1794
NlaIV GGNNCC 7 cut(s) 309, 690, 808, 816, 1469, 1560, 1655
NmuCI GTSAC 2 cut(s) 1635, 1847
NruI TCGCGA 1 cut(s) 350
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 744
PagI TCATGA 1 cut(s) 1620
PauI GCGCGC 2 cut(s) 6, 34
PfeI GAWTC 1 cut(s) 983
PflFI GACNNNGTC 1 cut(s) 121
PflMI CCANNNNNTGG 1 cut(s) 209
PkrI GCNGC 3 cut(s) 824, 1003, 1653
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
Psp1406I AACGTT 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 963, 1417
PspFI CCCAGC 1 cut(s) 1779
PspGI CCWGG 2 cut(s) 963, 1417
PspN4I GGNNCC 7 cut(s) 309, 690, 808, 816, 1469, 1560, 1655
PspPI GGNCC 3 cut(s) 368, 725, 1468
PsuI RGATCY 1 cut(s) 806
PsyI GACNNNGTC 1 cut(s) 121
PteI GCGCGC 2 cut(s) 6, 34
RruI TCGCGA 1 cut(s) 350
RsaI GTAC 4 cut(s) 918, 995, 1184, 1509
RsaNI GTAC 4 cut(s) 917, 994, 1183, 1508
RseI CAYNNNNRTG 1 cut(s) 1392
SaqAI TTAA 3 cut(s) 137, 341, 600
SatI GCNGC 3 cut(s) 823, 1002, 1652
Sau3AI GATC 3 cut(s) 806, 1593, 1794
Sau96I GGNCC 3 cut(s) 368, 725, 1468
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 3 cut(s) 811, 965, 1419
SfaNI GCATC 2 cut(s) 1012, 1195
SfcI CTRYAG 3 cut(s) 732, 1032, 1209
SinI GGWCC 2 cut(s) 368, 725
SmiMI CAYNNNNRTG 1 cut(s) 1392
SmlI CTYRAG 1 cut(s) 1990
SmoI CTYRAG 1 cut(s) 1990
SpeI ACTAGT 1 cut(s) 1811
Sse9I AATT 8 cut(s) 140, 181, 223, 355, 463, 536, 620, 956
SsiI CCGC 6 cut(s) 728, 968, 1008, 1651, 1766, 1987
SspI AATATT 1 cut(s) 1155
SspMI CTAG 4 cut(s) 24, 387, 693, 1812
StyD4I CCNGG 3 cut(s) 809, 963, 1417
StyI CCWWGG 2 cut(s) 873, 1061
TaaI ACNGT 4 cut(s) 164, 486, 1373, 1760
TaiI ACGT 4 cut(s) 125, 299, 558, 1683
TaqI TCGA 2 cut(s) 273, 552
TaqII GACCGA 1 cut(s) 1691
TasI AATT 8 cut(s) 140, 181, 223, 355, 463, 536, 620, 956
TatI WGTACW 2 cut(s) 916, 993
TauI GCSGC 1 cut(s) 1654
TfiI GAWTC 1 cut(s) 983
Tru1I TTAA 3 cut(s) 137, 341, 600
Tru9I TTAA 3 cut(s) 137, 341, 600
TseFI GTSAC 2 cut(s) 1635, 1847
TseI GCWGC 2 cut(s) 822, 1001
Tsp45I GTSAC 2 cut(s) 1635, 1847
TspGWI ACGGA 2 cut(s) 360, 1803
Tth111I GACNNNGTC 1 cut(s) 121
Van91I CCANNNNNTGG 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 368, 725
XapI RAATTY 2 cut(s) 140, 463
XceI RCATGY 1 cut(s) 744
XcmI CCANNNNNNNNNTGG 1 cut(s) 1540
XspI CTAG 4 cut(s) 24, 387, 693, 1812
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.