MD08G1146200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008025
Physical Location & Seq
Forward (+)
14601542 .. 14602819
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1146200.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGACCTGTAGACATATAGAGAAGTTTATCTACGGTGCAAAGCATCTAATGGCTAAGCGAAATGGAGCGTTGCATGTGAATGTGAAGCTGTTTCATTGGTCGGAGGAGTCTCGTGAAGATCCTTTGCCCCCAGAGTGGTATCAGAAGGCATTCCCGAAGTTGACAAAATTAACCCAGTTGTTAAAAAATGTTGACTTGATTGATGGAAGGCTCGTCAATGTCAGTGATGATTCGATTATCATTGATGATCGTATTGAAAACAGAATGCTTACTTTCAAGTCACTTGCCAGGGTCTTTATTGGAGCTCCACTAGTACAGAAAGCGCTGAGGAATAATATGGCGGCATTGTCAGGAGGTAGAATCCATAACCCGTTTGTGTGTTTCGGTACACCTAGTGAAAGGGAGCCCATGATTGTGAATTCACTCTCAGTAGTAGGTGGCTTTCTGAACATCTCTGCCCAACAAAGGCAGTCGGTGCGTGTCACAATATCCCCACAGGTCACACAGCATCGCATATGGACAGGGATGCTTGAGGAGGTACTCAATGGATTGAAATCCGAGTTGGATTATCTAGACTATCAGTACCCAAGCAAAGGGATAAAAATGGGGCAACAGATAGCTTCTAGCTGTCTAAAGTTCTTGGCCGACACATCCACCTCCATTTCATATGAAAACGACTCCTCTTCATGGACACGCCTTGTGCCTGCCAAAGTTATTGACTCTTCTGGTTTGCAGAAATGGGAAGATGTTCTTGACATGTTCAGCGACCTCATTGATTGGTTGAAAAATGAAAGGAGGTTACTGACTTCCGTAGAAAAGCTTGAGGTAATGAAAGAGGGGCTGTGTCAAATCAAGGATGTGCTGACTGATAGAAGTATAGGACACAAGGAACTCCGGCATCAAGAAAGCTTAGTGCAGAAGAAGTTGACGAAGACATTGGGTCATTCATCCAAGTGCTTGTTCACACTTTTGTTATATTACCTGTATGGGCGTGTTGGAGATATTGAAGTGGACATGCGTGGTGGTATTTATAGCCGTGGTAGTGGGGATGATTTCTGCTTGTGCATGGCAAGGATTGTGACTTCGGACGAGGAGGAGATGGTGTGGAGCGGGGTGAGGCAGTTAGACAGGGCTCTTGGGCTTTTCAAGTTTGTATGGGAAACAGCAGGGATGAAAGGGGCTTTACAACTGCAAGGCCATGTATGGTGTGTAGGGGCTGAGGGCAGAGTTCTTACATATAGAGGAAACACCTTTTTTGTACATGGGATTCATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

48.24

Weight (kDa)

8.5

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1110
AccI GTMKAC 1 cut(s) 10
AciI CCGC 2 cut(s) 341, 1110
AclWI GGATC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 1 cut(s) 418
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 5 cut(s) 315, 388, 540, 584, 1260
AfeI AGCGCT 1 cut(s) 324
AfiI CCNNNNNNNGG 4 cut(s) 135, 465, 593, 687
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 6 cut(s) 257, 277, 553, 784, 1007, 1147
AhdI GACNNNNNGTC 1 cut(s) 939
AhlI ACTAGT 1 cut(s) 310
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 4 cut(s) 545, 577, 944, 976
AluBI AGCT 6 cut(s) 88, 305, 620, 627, 820, 909
AluI AGCT 6 cut(s) 88, 305, 620, 627, 820, 909
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 113
Aor51HI AGCGCT 1 cut(s) 324
AoxI GGCC 2 cut(s) 642, 1195
ApoI RAATTY 1 cut(s) 418
Asp700I GAANNNNTTC 2 cut(s) 149, 747
AspLEI GCGC 1 cut(s) 325
AsuHPI GGTGA 1 cut(s) 1126
BanII GRGCYC 3 cut(s) 307, 408, 1135
BarI GAAGNNNNNNTAC 2 cut(s) 14, 46
BauI CACGAG 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 938
Bbv12I GWGCWC 1 cut(s) 307
BbvCI CCTCAGC 2 cut(s) 326, 1218
BccI CCATC 2 cut(s) 197, 1093
BceAI ACGGC 1 cut(s) 1020
BciT130I CCWGG 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 114
BcuI ACTAGT 1 cut(s) 310
BfaI CTAG 4 cut(s) 311, 393, 572, 624
BfmI CTRYAG 1 cut(s) 7
BfoI RGCGCY 1 cut(s) 326
BisI GCNGC 1 cut(s) 342
BlpI GCTNAGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 289
BmeRI GACNNNNNGTC 1 cut(s) 939
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 289
BmrI ACTGGG 1 cut(s) 169
BmsI GCATC 4 cut(s) 52, 516, 517, 907
BmuI ACTGGG 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 938
Bpu10I CCTNAGC 2 cut(s) 326, 1218
Bpu1102I GCTNAGC 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 551, 842
BsaBI GATNNNNATC 1 cut(s) 553
BsaJI CCNNGG 2 cut(s) 288, 1036
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 465, 593, 687
Bse1I ACTGG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 553
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 288, 1036
BseGI GGATG 6 cut(s) 531, 650, 862, 947, 1054, 1176
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 4 cut(s) 135, 465, 593, 687
BseMII CTCAG 3 cut(s) 317, 441, 1209
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 5 cut(s) 119, 548, 670, 1106, 1109
BsgI GTGCAG 1 cut(s) 935
BshFI GGCC 2 cut(s) 644, 1197
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 895
BslI CCNNNNNNNGG 4 cut(s) 135, 465, 593, 687
BsmAI GTCTC 1 cut(s) 114
BsmI GAATGC 2 cut(s) 149, 270
BsnI GGCC 2 cut(s) 644, 1197
Bsp1286I GDGCHC 3 cut(s) 307, 408, 1135
Bsp1407I TGTACA 1 cut(s) 1258
Bsp143I GATC 2 cut(s) 118, 247
Bsp1720I GCTNAGC 1 cut(s) 54
BspACI CCGC 2 cut(s) 341, 1110
BspANI GGCC 2 cut(s) 644, 1197
BspCNI CTCAG 3 cut(s) 318, 440, 1210
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 1110
BsrGI TGTACA 1 cut(s) 1258
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 288, 1036
BssMI GATC 2 cut(s) 118, 247
BssSI CACGAG 1 cut(s) 111
Bst2BI CACGAG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 2 cut(s) 688, 727
BstAPI GCANNNNNTGC 1 cut(s) 475
BstAUI TGTACA 1 cut(s) 1258
BstC8I GCNNGC 1 cut(s) 705
BstDEI CTNAG 5 cut(s) 54, 326, 427, 910, 1218
BstDSI CCRYGG 1 cut(s) 1036
BstF5I GGATG 6 cut(s) 531, 650, 862, 947, 1054, 1176
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 2 cut(s) 121, 250
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 2 cut(s) 118, 247
BstMWI GCNNNNNNNGC 1 cut(s) 475
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 3 cut(s) 77, 760, 1018
BstSCI CCNGG 1 cut(s) 287
BstSFI CTRYAG 1 cut(s) 7
BstV2I GAAGAC 1 cut(s) 938
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 2 cut(s) 644, 1197
BtgI CCRYGG 1 cut(s) 1036
BtgZI GCGATG 1 cut(s) 494
BtsCI GGATG 6 cut(s) 531, 650, 862, 947, 1054, 1176
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 705
CfoI GCGC 1 cut(s) 325
Csp6I GTAC 5 cut(s) 314, 387, 539, 583, 1259
CviAII CATG 9 cut(s) 74, 409, 687, 757, 1015, 1066, 1199, 1262, 1271
CviQI GTAC 5 cut(s) 314, 387, 539, 583, 1259
DdeI CTNAG 5 cut(s) 54, 326, 427, 910, 1218
DpnI GATC 2 cut(s) 120, 249
DpnII GATC 2 cut(s) 118, 247
DraIII CACNNNGTG 1 cut(s) 395
DriI GACNNNNNGTC 1 cut(s) 939
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 2 cut(s) 688, 727
Eam1105I GACNNNNNGTC 1 cut(s) 939
EarI CTCTTC 2 cut(s) 688, 727
Ecl136II GAGCTC 1 cut(s) 305
Eco24I GRGCYC 3 cut(s) 307, 408, 1135
Eco47III AGCGCT 1 cut(s) 324
Eco53kI GAGCTC 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 287
EcoT38I GRGCYC 3 cut(s) 307, 408, 1135
FaeI CATG 9 cut(s) 77, 412, 690, 760, 1018, 1069, 1202, 1265, 1274
FalI AAGNNNNNCTT 2 cut(s) 735, 767
FatI CATG 9 cut(s) 73, 408, 686, 756, 1014, 1065, 1198, 1261, 1270
FauI CCCGC 1 cut(s) 1103
FauNDI CATATG 2 cut(s) 515, 667
FblI GTMKAC 1 cut(s) 10
Fnu4HI GCNGC 1 cut(s) 342
FokI GGATG 6 cut(s) 538, 637, 869, 934, 1061, 1183
FriOI GRGCYC 3 cut(s) 307, 408, 1135
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 4 cut(s) 311, 393, 572, 624
GlaI GCGC 1 cut(s) 324
GluI GCNGC 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 2 cut(s) 644, 1197
HapII CCGG 1 cut(s) 895
HhaI GCGC 1 cut(s) 325
Hin1II CATG 9 cut(s) 77, 412, 690, 760, 1018, 1069, 1202, 1265, 1274
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 3 cut(s) 162, 193, 927
HindII GTYRAC 3 cut(s) 162, 193, 927
HindIII AAGCTT 2 cut(s) 818, 907
HinfI GANTC 6 cut(s) 107, 230, 360, 677, 719, 1267
HpaII CCGG 1 cut(s) 895
HphI GGTGA 1 cut(s) 1126
Hpy166II GTNNAC 7 cut(s) 11, 162, 193, 389, 927, 963, 1012
Hpy188I TCNGA 5 cut(s) 103, 144, 447, 559, 1087
Hpy188III TCNNGA 6 cut(s) 113, 154, 351, 572, 752, 902
Hpy8I GTNNAC 7 cut(s) 11, 162, 193, 389, 927, 963, 1012
HpyAV CCTTC 2 cut(s) 139, 201
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4V TGCA 6 cut(s) 38, 73, 733, 916, 1065, 1192
HpyF10VI GCNNNNNNNGC 1 cut(s) 475
HpyF3I CTNAG 5 cut(s) 54, 326, 427, 910, 1218
Hsp92II CATG 9 cut(s) 77, 412, 690, 760, 1018, 1069, 1202, 1265, 1274
HspAI GCGC 1 cut(s) 323
Kzo9I GATC 2 cut(s) 118, 247
LmnI GCTCC 5 cut(s) 65, 302, 310, 403, 1107
LweI GCATC 4 cut(s) 52, 516, 517, 907
MaeI CTAG 4 cut(s) 311, 393, 572, 624
MaeIII GTNAC 5 cut(s) 279, 481, 499, 798, 1078
MalI GATC 2 cut(s) 120, 249
MbiI CCGCTC 1 cut(s) 1110
MboI GATC 2 cut(s) 118, 247
MboII GAAGA 6 cut(s) 128, 675, 714, 755, 931, 943
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 3 cut(s) 307, 408, 1135
MluCI AATT 2 cut(s) 167, 418
MlyI GAGTC 3 cut(s) 116, 671, 713
MmeI TCCRAC 3 cut(s) 81, 543, 976
MroXI GAANNNNTTC 2 cut(s) 149, 747
MseI TTAA 2 cut(s) 170, 182
MslI CAYNNNNRTG 3 cut(s) 78, 413, 952
MspI CCGG 1 cut(s) 895
MspR9I CCNGG 1 cut(s) 289
Mva1269I GAATGC 2 cut(s) 149, 270
MvaI CCWGG 1 cut(s) 289
MwoI GCNNNNNNNGC 1 cut(s) 475
NdeI CATATG 2 cut(s) 515, 667
NdeII GATC 2 cut(s) 118, 247
NlaIII CATG 9 cut(s) 77, 412, 690, 760, 1018, 1069, 1202, 1265, 1274
NlaIV GGNNCC 1 cut(s) 405
NmuCI GTSAC 4 cut(s) 279, 481, 499, 1078
NspI RCATGY 3 cut(s) 77, 760, 1018
PciI ACATGT 1 cut(s) 756
PctI GAATGC 2 cut(s) 149, 270
PdmI GAANNNNTTC 2 cut(s) 149, 747
PfeI GAWTC 3 cut(s) 230, 360, 1267
PkrI GCNGC 1 cut(s) 343
PleI GAGTC 3 cut(s) 115, 671, 713
PpsI GAGTC 3 cut(s) 115, 671, 713
PscI ACATGT 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 1 cut(s) 405
PsuI RGATCY 1 cut(s) 118
RsaI GTAC 5 cut(s) 315, 388, 540, 584, 1260
RsaNI GTAC 5 cut(s) 314, 387, 539, 583, 1259
RseI CAYNNNNRTG 3 cut(s) 78, 413, 952
SacI GAGCTC 1 cut(s) 307
SaqAI TTAA 2 cut(s) 170, 182
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 2 cut(s) 118, 247
SchI GAGTC 3 cut(s) 116, 671, 713
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 3 cut(s) 307, 408, 1135
SfaNI GCATC 4 cut(s) 52, 516, 517, 907
SfcI CTRYAG 1 cut(s) 7
SmiMI CAYNNNNRTG 3 cut(s) 78, 413, 952
SmlI CTYRAG 2 cut(s) 530, 821
SmoI CTYRAG 2 cut(s) 530, 821
SpeI ACTAGT 1 cut(s) 310
Sse9I AATT 2 cut(s) 167, 418
SsiI CCGC 2 cut(s) 341, 1110
SspMI CTAG 4 cut(s) 311, 393, 572, 624
SstI GAGCTC 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 287
TaaI ACNGT 1 cut(s) 35
TaqI TCGA 1 cut(s) 233
TasI AATT 2 cut(s) 167, 418
TatI WGTACW 2 cut(s) 313, 1258
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 3 cut(s) 230, 360, 1267
Tru1I TTAA 2 cut(s) 170, 182
Tru9I TTAA 2 cut(s) 170, 182
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 4 cut(s) 279, 481, 499, 1078
Tsp45I GTSAC 4 cut(s) 279, 481, 499, 1078
TspDTI ATGAA 9 cut(s) 83, 654, 675, 684, 804, 845, 936, 1187, 1259
TspGWI ACGGA 1 cut(s) 799
TspRI CASTG 1 cut(s) 229
XapI RAATTY 1 cut(s) 418
XbaI TCTAGA 1 cut(s) 571
XceI RCATGY 3 cut(s) 77, 760, 1018
XmiI GTMKAC 1 cut(s) 10
XmnI GAANNNNTTC 2 cut(s) 149, 747
XspI CTAG 4 cut(s) 311, 393, 572, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.