Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002335

Physical Location & Seq

Genomic Coordinates Forward (+)

2336668 .. 2342644

Gene Structure 5977 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1038400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2946 bp

ATGGCGGACGGCAGAGATGCGGCGGAAGGAAGCAACGCCATAGCAAGCACGCGGAGAAGACGACGGATATGGCTGAAGAGAAAAGAGAAATGGCTGGTAGTACTGGGCGTAGTTCTCCACGCTGTTTACATGCTCAGCATCTTTGACATCTACTTCAAGTCCCCCATTGTCCACGGCATGGATCCCGTCACCCCCCGCTTCTCCGCTCCTGCAAAACGCCTCGTTTTGCTCGTCGCTGATGGGCTGCGAGCCGACAAATTCTTCGAGTCGGATTCCGACGGGAACTTCAGAGCACCGTTTTTGAGAAGTGTTATGAGAGAGAAAGGTCGGTGGGGAGTGTCGCACGCTCGTCCGCCGACGGAGTCGAGGCCTGGACATGTTGCTATCATTGCTGGGTTCTATGAGGATCCCAGTGCTGTTACGAAAGGGTGGAAAGCTAACCCTGTTGAATTCGATTCGGTTTTTAATCGAAGCCGGCATACTTTTTCTTATGGTAGTCCGGATATAGTTCCGATTTTCTGCGCTGCATTGCCTCACAGCACCTGGAATAGTTACCCTCATGAATTTGAAGACTTTGCTACTGATGCTTCCTTTCTGGATGAGTGGTCTTTTGATCAATTTCAGAGCCTTCTGAATCGGTCCAAAGAAGACCAGAAGTTGAAGGAGTTACTCTTACAGGATAATCTTGTTGTGTTTTTGCACTTACTTGGCTGTGATTCAAATGGTCATGCGCATAGGCCCTTTTCGTCCATATATCTCAATAATGTTGCGGTTGTTGACAGTATAGCTGAAAGGGTTTATAATCTTCTTGAAGATTATTACAAGGACAATTGTACTGCATATGTATTTACCGCTGATCATGGAATGCATGATAAAGGCAGCCATGGAGATGGACATCCTTCAAACACTGATACTCCCCTAGTTGTTTGGGGAGCAGGTGTTAAACATCCCAAGCCAGTCTCCAACCATTCTGATTGCAGTTTTCGTTTTGTTGATGATCATATGCATGATGCGCCAACACCTAAAGAATGGGGCCTTCATGGCATTGAAAGGGTGGATGTTAATCAAGCTGATATTGCACCTCTCATGGTTTCAACTCTTCTTGGTTTGCCTGGTCCTGTAAACTCAGTTGGAAGTTTACCTCTTGATTACATAGACATGACAAAGGAAGATGAAGCTGAAGCTGTGGTTGCAAACTCAAAGCAAATTCTCAACCAGTTTCTTAGGAAGTCACAAACAAAGGAGGCAAATTCATTAAATTTTAAGCCTTTCAAACCACTGGCCGATTATTCCTCCCTATTGGATCAGATTGAGGATCTAATATCTAATACAGACTATGAAGCTGCAAGGAAGCTGTCTGAAGATCTCAGGGATTTGGCTTTGAAAGGACTACACTATTTCCAAACTTACGATTGGCTGATGCTCATGACAGTAATTATTCTTGGGTATATCGGTTGGATGACCTATATTCTTCTCCATGTGTTGCAATCTTATACTTCATTGGCAGGGTATATGTTTAGAAAGGAGCAAGCAGATCACCGGACAGATAATACCAGAAAAGTACAACTTTGTGGATGCCTTTTTCTGGGACTACTTAGTATAGTACTGTTTATGGAGCGTTCTCCTCCTCTTTACCACACATACACGTCAATGACAGTATTTCTATGGACACAAATATTTAGTGAATATTGGTTCATCAAAGCATTATGGAAAGATTTATATGGAAGAAGAATCAATTACTTTGTGAAAATTCTAGCCACTGGTGCTTTCTCAGTATTCATTCTTGAATTCCTGGTTAATAGCTTTACTGAGAGGAAGCTATACACTTGGTGTTTCCTAGTTTCCGGAGTCATATCCTTTCTCTATCTCTTAAAATCAATTCCATGGAGATCTGGCATACCAATCTTTGTTTGTGCTGCATGTTGGTTCTTGTCTGTATTTACTTTGATGCCAGCAGAAATTCCAGATAATAATCGTCTAGTGATTTGGAGTGGAGTTATGATTATTATGATCGGAGTAGCTGCCAGACTGCTAGATCTGCGCACTGAAGGAAAGTATTGGCTAAGTATCTGTAATCATGACAAGAAGAAACCTAAATTTCCCATGCTCTTCCAGTTACAGGCTCTTTTGGTTGGATTGTCATCCATGATGGTGTCGATATCAACCTCTCATAGAACAGAGAAGCAGGAACTACTCGCTGTGCACCAGATCATAAATTGGTCCATCGCTGGTTTCTCGATGGTACTTCCATTATTTTCAGCAAATGGTCTCTTGTCCCGGCTTACTTCCATATTTCTCGGTTTTGCTCCCACATTTCTTCTATTATCTATCGGATATGAAGCTGTTTTCTACGGTGCTCTGGCCCTTGTACTCATCTCTTGGATACTAGTTGAAAACACACTTATTTACTCAAGTAAGGTGAAAAGGTTGTCAAGTTCCTTTAGCGATATGGAGGATAATGTCATACTTGATGGTAGATACTTACAGCTGTCTGACGTGAGAATTCCGTTAATCTTTATGGTTTTATTCAACGTTGCTTTCTTTGGAACGGGAAACTTTGCGAGTATTGCAAGTTTTGAGATTTCATCAGTGTATCGGTTCATTACTGTCTTCAGTCCGTTTCTCATGGCCGCCTTACTTATCTTCAAGTTATTCATTCCGTTCCTGCTAGTCATATGTGTATTCAGTGCAATAACAAAGCTAAACCGACTTCCACGGTTGGCATGCTATTTTCTCGTTATACTATTTTCAGACGTAATGACTATGCATTTTTTCTTCCTGGTCCGGAATACAGGAAGCTGGATGGAAATTGGTAACAGCATCAGCCACTTCGGAATCATGAGTGCTCAAGTTGTCTTTGTGCTTTTGCTCTTTGCTCTCACGAACATATACACGAAAGATATCGACATTGGTTCAGTGGACTGCTCCTCTCGGAAAGCAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 map00563 map01100

Pfam Domains

Protein Families

Phosphodiest PigN Sulfatase

Protein Analysis

982

Amino Acids

111.4

Weight (kDa)

7.15

Isoelectric Point (pI)

36.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphodiest	PF01663	178 - 291	9.7e-06	Type I phosphodiesterase / nucleotide pyrophosphatase
Sulfatase	PF00884	248 - 323	1.9e-06	Sulfatase
PigN	PF04987	462 - 933	7.9e-127	Phosphatidylinositolglycan class N (PIG-N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002335)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01380 AT3G01380
fragaria_vesca	FvH4_6g50180 FvH4_6g50180 FvH4_6g50180
malus_domestica	MD09G1038400.v1.1 MD17G1039500.v1.1
prunus_persica	Prupe.2G019300_v2.0.a1 Prupe.3G279400_v2.0.a1 Prupe.3G279400_v2.0.a1
pyrus_communis	pycom111g03060 pycom17g03550 pycom17g03560
rosa_chinensis	RchiOBHm_Chr2g0170441 RchiOBHm_Chr7g0219531 RchiOBHm_Chr7g0219551 RchiOBHm_Chr7g0219561 RchiOBHm_Chr7g0219571 RchiOBHm_Chr7g0219581
rosa_laevigata	RLG00000002284 RLG00000021957
rosa_multiflora	Rmu_co7962372.1_g000001 Rmu_sc0009395.1_g000013
rosa_roxburghii	Rroxscaffold_2G00081260 Rroxscaffold_2G00081270 Rroxscaffold_3G00239980
rosa_rugosa	Rorug02G0548900 Rorug07G0186100 Rorug07G0186200 Rorug07G0186300
rosa_samantha	Rh2AG620800 Rh2BG631800 Rh2CG601400 Rh2DG644500 Rh7AG330000 Rh7AG330100 Rh7BG319700 Rh7BG319800 Rh7CG347300 Rh7CG347400
rosa_wichuraiana	Rw2G051460 Rw7G027790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	801
AarI	CACCTGC	1 cut(s)	926
Acc16I	TGCGCA	2 cut(s)	732, 2042
Acc36I	ACCTGC	1 cut(s)	926
AccB7I	CCANNNNNTGG	1 cut(s)	178
AccBSI	CCGCTC	1 cut(s)	206
AccII	CGCG	1 cut(s)	52
AccIII	TCCGGA	3 cut(s)	499, 1844, 2784
AclI	AACGTT	1 cut(s)	2532
AclWI	GGATC	6 cut(s)	176, 189, 401, 414, 1311, 1323
AcoI	YGGCCR	2 cut(s)	1281, 2628
AcuI	CTGAAG	6 cut(s)	95, 271, 1200, 1380, 2067, 2596
AdeI	CACNNNGTG	1 cut(s)	1830
AfaI	GTAC	6 cut(s)	102, 835, 1563, 1605, 2244, 2370
AfiI	CCNNNNNNNGG	3 cut(s)	178, 1585, 2119
AflIII	ACRYGT	2 cut(s)	376, 1644
AhlI	ACTAGT	1 cut(s)	2386
AjiI	CACGTC	2 cut(s)	1647, 2497
AjnI	CCWGG	5 cut(s)	370, 542, 1111, 1791, 2778
Alw21I	GWGCWC	4 cut(s)	295, 2205, 2359, 2848
Alw26I	GTCTC	2 cut(s)	964, 2273
Alw44I	GTGCAC	1 cut(s)	2201
AlwI	GGATC	6 cut(s)	176, 189, 401, 414, 1311, 1323
AlwNI	CAGNNNCTG	1 cut(s)	543
Aor13HI	TCCGGA	3 cut(s)	499, 1844, 2784
AoxI	GGCC	6 cut(s)	368, 737, 1033, 1281, 2361, 2628
ApaLI	GTGCAC	1 cut(s)	2201
ApeKI	GCWGC	6 cut(s)	244, 524, 879, 1343, 1916, 2021
ArsI	GACNNNNNNTTYG	2 cut(s)	245, 277
AspLEI	GCGC	4 cut(s)	524, 733, 1015, 2043
AspS9I	GGNCC	7 cut(s)	639, 738, 1033, 1115, 2220, 2362, 2782
AsuC2I	CCSGG	1 cut(s)	2278
AsuHPI	GGTGA	3 cut(s)	181, 1529, 2431
AvaII	GGWCC	4 cut(s)	639, 1115, 2220, 2782
BaeGI	GKGCMC	1 cut(s)	2205
BamHI	GGATCC	2 cut(s)	181, 406
BarI	GAAGNNNNNNTAC	2 cut(s)	463, 495
BbsI	GAAGAC	4 cut(s)	64, 576, 654, 2602
Bbv12I	GWGCWC	4 cut(s)	295, 2205, 2359, 2848
BbvI	GCAGC	6 cut(s)	231, 511, 891, 1330, 1903, 2008
BccI	CCATC	7 cut(s)	233, 884, 2143, 2231, 2233, 2465, 2797
BceAI	ACGGC	2 cut(s)	25, 190
BciT130I	CCWGG	5 cut(s)	372, 544, 1113, 1793, 2780
BciVI	GTATCC	1 cut(s)	2376
BclI	TGATCA	3 cut(s)	613, 856, 997
BcnI	CCSGG	1 cut(s)	2278
BcoDI	GTCTC	2 cut(s)	964, 2273
BcuI	ACTAGT	1 cut(s)	2386
BfaI	CTAG	7 cut(s)	920, 1754, 1838, 1979, 2033, 2387, 2669
BfuAI	ACCTGC	1 cut(s)	926
BfuI	GTATCC	1 cut(s)	2376
BglI	GCCNNNNNGGC	1 cut(s)	1041
BglII	AGATCT	3 cut(s)	1363, 1889, 2035
BisI	GCNGC	8 cut(s)	21, 245, 525, 880, 1344, 1917, 2022, 2631
BlpI	GCTNAGC	1 cut(s)	134
BlsI	GCNGC	8 cut(s)	22, 246, 526, 881, 1345, 1918, 2023, 2632
BmcAI	AGTACT	2 cut(s)	102, 1605
Bme1390I	CCNGG	6 cut(s)	372, 544, 1113, 1793, 2278, 2780
Bme18I	GGWCC	4 cut(s)	639, 1115, 2220, 2782
BmgBI	CACGTC	2 cut(s)	1647, 2497
BmgT120I	GGNCC	7 cut(s)	639, 738, 1033, 1115, 2220, 2362, 2782
BmiI	GGNNCC	3 cut(s)	183, 408, 1034
BmrFI	CCNGG	6 cut(s)	372, 544, 1113, 1793, 2278, 2780
BmrI	ACTGGG	2 cut(s)	113, 405
BmsI	GCATC	8 cut(s)	7, 147, 574, 1000, 1410, 1565, 1938, 2829
BmuI	ACTGGG	2 cut(s)	113, 405
BpiI	GAAGAC	4 cut(s)	64, 576, 654, 2602
Bpu1102I	GCTNAGC	1 cut(s)	134
BpuEI	CTTGAG	2 cut(s)	2395, 2832
BpuMI	CCSGG	1 cut(s)	2278
BsaBI	GATNNNNATC	4 cut(s)	894, 1062, 1971, 2140
BsaI	GGTCTC	1 cut(s)	2273
BsaJI	CCNNGG	4 cut(s)	172, 883, 1883, 2714
BsaWI	WCCGGW	4 cut(s)	499, 1539, 1844, 2784
BsaXI	ACNNNNNCTCC	4 cut(s)	898, 924, 928, 954
Bsc4I	CCNNNNNNNGG	3 cut(s)	178, 1585, 2119
Bse118I	RCCGGY	1 cut(s)	474
Bse1I	ACTGG	7 cut(s)	108, 411, 956, 1216, 1284, 1765, 2113
Bse3DI	GCAATG	3 cut(s)	387, 527, 2946
Bse8I	GATNNNNATC	4 cut(s)	894, 1062, 1971, 2140
BseAI	TCCGGA	3 cut(s)	499, 1844, 2784
BseBI	CCWGG	5 cut(s)	372, 544, 1113, 1793, 2780
BseDI	CCNNGG	4 cut(s)	172, 883, 1883, 2714
BseGI	GGATG	8 cut(s)	604, 895, 946, 1063, 1464, 1580, 2141, 2808
BseJI	GATNNNNATC	4 cut(s)	894, 1062, 1971, 2140
BseLI	CCNNNNNNNGG	3 cut(s)	178, 1585, 2119
BseMI	GCAATG	3 cut(s)	387, 527, 2946
BseMII	CTCAG	5 cut(s)	148, 1140, 1381, 1785, 1800
BseNI	ACTGG	7 cut(s)	108, 411, 956, 1216, 1284, 1765, 2113
BseRI	GAGGAG	3 cut(s)	1614, 1617, 2917
BseSI	GKGCMC	1 cut(s)	2205
BseXI	GCAGC	6 cut(s)	231, 511, 891, 1330, 1903, 2008
BseYI	CCCAGC	1 cut(s)	392
Bsh1236I	CGCG	1 cut(s)	52
BshFI	GGCC	6 cut(s)	370, 739, 1035, 1283, 2363, 2630
BsiHKAI	GWGCWC	4 cut(s)	295, 2205, 2359, 2848
BsiSI	CCGG	6 cut(s)	475, 500, 1540, 1845, 2278, 2785
BslFI	GGGAC	3 cut(s)	145, 1602, 2260
BslI	CCNNNNNNNGG	3 cut(s)	178, 1585, 2119
BsmAI	GTCTC	2 cut(s)	964, 2273
BsmFI	GGGAC	3 cut(s)	145, 1602, 2260
BsmI	GAATGC	1 cut(s)	870
BsnI	GGCC	6 cut(s)	370, 739, 1035, 1283, 2363, 2630
Bso31I	GGTCTC	1 cut(s)	2273
Bsp1286I	GDGCHC	4 cut(s)	295, 2205, 2359, 2848
Bsp13I	TCCGGA	3 cut(s)	499, 1844, 2784
Bsp1720I	GCTNAGC	1 cut(s)	134
Bsp19I	CCATGG	2 cut(s)	883, 1883
BspANI	GGCC	6 cut(s)	370, 739, 1035, 1283, 2363, 2630
BspCNI	CTCAG	5 cut(s)	147, 1139, 1380, 1784, 1801
BspEI	TCCGGA	3 cut(s)	499, 1844, 2784
BspFNI	CGCG	1 cut(s)	52
BspHI	TCATGA	4 cut(s)	559, 1425, 2077, 2838
BspLI	GGNNCC	3 cut(s)	183, 408, 1034
BspMI	ACCTGC	1 cut(s)	926
BspPI	GGATC	6 cut(s)	176, 189, 401, 414, 1311, 1323
BspQI	GCTCTTC	1 cut(s)	2114
BspTNI	GGTCTC	1 cut(s)	2273
BsrBI	CCGCTC	1 cut(s)	206
BsrDI	GCAATG	3 cut(s)	387, 527, 2946
BsrFI	RCCGGY	1 cut(s)	474
BsrI	ACTGG	7 cut(s)	108, 411, 956, 1216, 1284, 1765, 2113
BssAI	RCCGGY	1 cut(s)	474
BssECI	CCNNGG	4 cut(s)	172, 883, 1883, 2714
BssT1I	CCWWGG	2 cut(s)	883, 1883
Bst2UI	CCWGG	5 cut(s)	372, 544, 1113, 1793, 2780
Bst4CI	ACNGT	8 cut(s)	297, 782, 1432, 1608, 1657, 2354, 2608, 2718
Bst6I	CTCTTC	3 cut(s)	71, 1104, 2114
BstC8I	GCNNGC	8 cut(s)	46, 50, 249, 345, 476, 1530, 1953, 2725
BstDEI	CTNAG	8 cut(s)	134, 1126, 1223, 1367, 1595, 1771, 1809, 2063
BstDSI	CCRYGG	4 cut(s)	172, 883, 1883, 2714
BstF5I	GGATG	8 cut(s)	604, 895, 946, 1063, 1464, 1580, 2141, 2808
BstFNI	CGCG	1 cut(s)	52
BstHHI	GCGC	4 cut(s)	524, 733, 1015, 2043
BstMAI	GTCTC	2 cut(s)	964, 2273
BstMWI	GCNNNNNNNGC	9 cut(s)	389, 584, 1012, 1041, 1076, 1190, 1763, 2038, 2567
BstNI	CCWGG	5 cut(s)	372, 544, 1113, 1793, 2780
BstNSI	RCATGY	4 cut(s)	133, 380, 1923, 2727
BstSCI	CCNGG	6 cut(s)	370, 542, 1111, 1791, 2276, 2778
BstSLI	GKGCMC	1 cut(s)	2205
BstUI	CGCG	1 cut(s)	52
BstV1I	GCAGC	6 cut(s)	231, 511, 891, 1330, 1903, 2008
BstV2I	GAAGAC	4 cut(s)	64, 576, 654, 2602
BstX2I	RGATCY	6 cut(s)	181, 406, 1315, 1363, 1889, 2035
BstXI	CCANNNNNNTGG	1 cut(s)	890
BstYI	RGATCY	6 cut(s)	181, 406, 1315, 1363, 1889, 2035
BsuI	GTATCC	1 cut(s)	2376
BsuRI	GGCC	6 cut(s)	370, 739, 1035, 1283, 2363, 2630
BtgI	CCRYGG	4 cut(s)	172, 883, 1883, 2714
BtgZI	GCGATG	1 cut(s)	2209
BtrI	CACGTC	2 cut(s)	1647, 2497
BtsCI	GGATG	8 cut(s)	604, 895, 946, 1063, 1464, 1580, 2141, 2808
BtsIMutI	CAGTG	8 cut(s)	418, 906, 1277, 1758, 2043, 2595, 2692, 2922
BveI	ACCTGC	1 cut(s)	926
Cac8I	GCNNGC	8 cut(s)	46, 50, 249, 345, 476, 1530, 1953, 2725
CaiI	CAGNNNCTG	1 cut(s)	543
CciI	TCATGA	4 cut(s)	559, 1425, 2077, 2838
CfoI	GCGC	4 cut(s)	524, 733, 1015, 2043
Cfr10I	RCCGGY	1 cut(s)	474
Cfr13I	GGNCC	7 cut(s)	639, 738, 1033, 1115, 2220, 2362, 2782
Csp6I	GTAC	6 cut(s)	101, 834, 1562, 1604, 2243, 2369
CviQI	GTAC	6 cut(s)	101, 834, 1562, 1604, 2243, 2369
DdeI	CTNAG	8 cut(s)	134, 1126, 1223, 1367, 1595, 1771, 1809, 2063
DraIII	CACNNNGTG	1 cut(s)	1830
EaeI	YGGCCR	2 cut(s)	1281, 2628
Eam1104I	CTCTTC	3 cut(s)	71, 1104, 2114
EarI	CTCTTC	3 cut(s)	71, 1104, 2114
EciI	GGCGGA	3 cut(s)	20, 38, 342
Eco130I	CCWWGG	2 cut(s)	883, 1883
Eco147I	AGGCCT	1 cut(s)	370
Eco31I	GGTCTC	1 cut(s)	2273
Eco32I	GATATC	2 cut(s)	2160, 2902
Eco47I	GGWCC	4 cut(s)	639, 1115, 2220, 2782
Eco57I	CTGAAG	6 cut(s)	95, 271, 1200, 1380, 2067, 2596
EcoO109I	RGGNCCY	2 cut(s)	738, 1033
EcoRI	GAATTC	3 cut(s)	449, 1787, 2502
EcoRII	CCWGG	5 cut(s)	370, 542, 1111, 1791, 2778
EcoRV	GATATC	2 cut(s)	2160, 2902
EcoT14I	CCWWGG	2 cut(s)	883, 1883
EcoT22I	ATGCAT	3 cut(s)	870, 1008, 2769
ErhI	CCWWGG	2 cut(s)	883, 1883
FalI	AAGNNNNNCTT	2 cut(s)	1551, 1583
FaqI	GGGAC	3 cut(s)	145, 1602, 2260
FauI	CCCGC	1 cut(s)	203
FauNDI	CATATG	3 cut(s)	841, 1002, 2675
FbaI	TGATCA	3 cut(s)	613, 856, 997
Fnu4HI	GCNGC	8 cut(s)	21, 245, 525, 880, 1344, 1917, 2022, 2631
FokI	GGATG	8 cut(s)	611, 882, 933, 1070, 1471, 1587, 2128, 2815
Fsp4HI	GCNGC	8 cut(s)	21, 245, 525, 880, 1344, 1917, 2022, 2631
FspAI	RTGCGCAY	1 cut(s)	732
FspBI	CTAG	7 cut(s)	920, 1754, 1838, 1979, 2033, 2387, 2669
FspI	TGCGCA	2 cut(s)	732, 2042
GlaI	GCGC	4 cut(s)	523, 732, 1014, 2042
GluI	GCNGC	8 cut(s)	21, 245, 525, 880, 1344, 1917, 2022, 2631
GsaI	CCCAGC	1 cut(s)	396
HaeIII	GGCC	6 cut(s)	370, 739, 1035, 1283, 2363, 2630
HapII	CCGG	6 cut(s)	475, 500, 1540, 1845, 2278, 2785
HhaI	GCGC	4 cut(s)	524, 733, 1015, 2043
Hin6I	GCGC	4 cut(s)	522, 731, 1013, 2041
HinP1I	GCGC	4 cut(s)	522, 731, 1013, 2041
HincII	GTYRAC	1 cut(s)	778
HindII	GTYRAC	1 cut(s)	778
HinfI	GANTC	9 cut(s)	266, 272, 362, 455, 634, 716, 1731, 1848, 2835
HpaII	CCGG	6 cut(s)	475, 500, 1540, 1845, 2278, 2785
HphI	GGTGA	3 cut(s)	181, 1529, 2431
Hpy166II	GTNNAC	7 cut(s)	127, 172, 778, 1123, 1139, 2203, 2920
Hpy8I	GTNNAC	7 cut(s)	127, 172, 778, 1123, 1139, 2203, 2920
Hpy99I	CGWCG	4 cut(s)	66, 236, 281, 361
HpyAV	CCTTC	6 cut(s)	20, 638, 655, 909, 1046, 2042
HpyCH4III	ACNGT	8 cut(s)	297, 782, 1432, 1608, 1657, 2354, 2608, 2718
HpyCH4IV	ACGT	4 cut(s)	1646, 2496, 2532, 2754
HpyF10VI	GCNNNNNNNGC	9 cut(s)	389, 584, 1012, 1041, 1076, 1190, 1763, 2038, 2567
HpyF3I	CTNAG	8 cut(s)	134, 1126, 1223, 1367, 1595, 1771, 1809, 2063
HpySE526I	ACGT	4 cut(s)	1646, 2496, 2532, 2754
HspAI	GCGC	4 cut(s)	522, 731, 1013, 2041
Kpn2I	TCCGGA	3 cut(s)	499, 1844, 2784
KroI	GCCGGC	1 cut(s)	474
KroNI	GCCGGC	1 cut(s)	476
Ksp22I	TGATCA	3 cut(s)	613, 856, 997
LguI	GCTCTTC	1 cut(s)	2114
LmnI	GCTCC	6 cut(s)	211, 932, 1525, 1615, 2311, 2930
Lsp1109I	GCAGC	6 cut(s)	231, 511, 891, 1330, 1903, 2008
LweI	GCATC	8 cut(s)	7, 147, 574, 1000, 1410, 1565, 1938, 2829
MaeI	CTAG	7 cut(s)	920, 1754, 1838, 1979, 2033, 2387, 2669
MaeII	ACGT	4 cut(s)	1646, 2496, 2532, 2754
MaeIII	GTNAC	7 cut(s)	187, 418, 551, 666, 1230, 2115, 2813
MbiI	CCGCTC	1 cut(s)	206
MfeI	CAATTG	1 cut(s)	829
MflI	RGATCY	6 cut(s)	181, 406, 1315, 1363, 1889, 2035
MhlI	GDGCHC	4 cut(s)	295, 2205, 2359, 2848
MlyI	GAGTC	3 cut(s)	275, 371, 1857
MmeI	TCCRAC	6 cut(s)	249, 300, 987, 1111, 1436, 2113
Mph1103I	ATGCAT	3 cut(s)	870, 1008, 2769
MroI	TCCGGA	3 cut(s)	499, 1844, 2784
MroNI	GCCGGC	1 cut(s)	474
MseI	TTAA	8 cut(s)	465, 942, 1062, 1256, 1263, 1797, 1871, 2510
MslI	CAYNNNNRTG	5 cut(s)	888, 1005, 1157, 1649, 2150
MspA1I	CMGCKG	2 cut(s)	854, 2488
MspI	CCGG	6 cut(s)	475, 500, 1540, 1845, 2278, 2785
MspR9I	CCNGG	6 cut(s)	372, 544, 1113, 1793, 2278, 2780
MunI	CAATTG	1 cut(s)	829
Mva1269I	GAATGC	1 cut(s)	870
MvaI	CCWGG	5 cut(s)	372, 544, 1113, 1793, 2780
MvnI	CGCG	1 cut(s)	52
MwoI	GCNNNNNNNGC	9 cut(s)	389, 584, 1012, 1041, 1076, 1190, 1763, 2038, 2567
NaeI	GCCGGC	1 cut(s)	476
NciI	CCSGG	1 cut(s)	2278
NcoI	CCATGG	2 cut(s)	883, 1883
NdeI	CATATG	3 cut(s)	841, 1002, 2675
NgoMIV	GCCGGC	1 cut(s)	474
NlaIV	GGNNCC	3 cut(s)	183, 408, 1034
NmuCI	GTSAC	2 cut(s)	187, 1230
NsbI	TGCGCA	2 cut(s)	732, 2042
NsiI	ATGCAT	3 cut(s)	870, 1008, 2769
NspI	RCATGY	4 cut(s)	133, 380, 1923, 2727
PaeI	GCATGC	1 cut(s)	2727
PagI	TCATGA	4 cut(s)	559, 1425, 2077, 2838
PaqCI	CACCTGC	1 cut(s)	926
PceI	AGGCCT	1 cut(s)	370
PciI	ACATGT	1 cut(s)	376
PciSI	GCTCTTC	1 cut(s)	2114
PcsI	WCGNNNNNNNCGW	1 cut(s)	228
PctI	GAATGC	1 cut(s)	870
PdiI	GCCGGC	1 cut(s)	476
PfeI	GAWTC	6 cut(s)	272, 455, 634, 716, 1731, 2835
PflFI	GACNNNGTC	1 cut(s)	361
PflMI	CCANNNNNTGG	1 cut(s)	178
PkrI	GCNGC	8 cut(s)	22, 246, 526, 881, 1345, 1918, 2023, 2632
PleI	GAGTC	3 cut(s)	274, 370, 1856
PpsI	GAGTC	3 cut(s)	274, 370, 1856
PscI	ACATGT	1 cut(s)	376
PsiI	TTATAA	1 cut(s)	801
Psp1406I	AACGTT	1 cut(s)	2532
Psp6I	CCWGG	5 cut(s)	370, 542, 1111, 1791, 2778
PspFI	CCCAGC	1 cut(s)	392
PspGI	CCWGG	5 cut(s)	370, 542, 1111, 1791, 2778
PspN4I	GGNNCC	3 cut(s)	183, 408, 1034
PspPI	GGNCC	7 cut(s)	639, 738, 1033, 1115, 2220, 2362, 2782
PstNI	CAGNNNCTG	1 cut(s)	543
PsuI	RGATCY	6 cut(s)	181, 406, 1315, 1363, 1889, 2035
PsyI	GACNNNGTC	1 cut(s)	361
PvuII	CAGCTG	1 cut(s)	2488
RsaI	GTAC	6 cut(s)	102, 835, 1563, 1605, 2244, 2370
RsaNI	GTAC	6 cut(s)	101, 834, 1562, 1604, 2243, 2369
RseI	CAYNNNNRTG	5 cut(s)	888, 1005, 1157, 1649, 2150
SapI	GCTCTTC	1 cut(s)	2114
SaqAI	TTAA	8 cut(s)	465, 942, 1062, 1256, 1263, 1797, 1871, 2510
SatI	GCNGC	8 cut(s)	21, 245, 525, 880, 1344, 1917, 2022, 2631
Sau96I	GGNCC	7 cut(s)	639, 738, 1033, 1115, 2220, 2362, 2782
ScaI	AGTACT	2 cut(s)	102, 1605
SchI	GAGTC	3 cut(s)	275, 371, 1857
ScrFI	CCNGG	6 cut(s)	372, 544, 1113, 1793, 2278, 2780
SduI	GDGCHC	4 cut(s)	295, 2205, 2359, 2848
SfaNI	GCATC	8 cut(s)	7, 147, 574, 1000, 1410, 1565, 1938, 2829
SinI	GGWCC	4 cut(s)	639, 1115, 2220, 2782
SmiMI	CAYNNNNRTG	5 cut(s)	888, 1005, 1157, 1649, 2150
SmlI	CTYRAG	2 cut(s)	2410, 2847
SmoI	CTYRAG	2 cut(s)	2410, 2847
SpeI	ACTAGT	1 cut(s)	2386
SphI	GCATGC	1 cut(s)	2727
SseBI	AGGCCT	1 cut(s)	370
SspI	AATATT	2 cut(s)	1677, 1688
SspMI	CTAG	7 cut(s)	920, 1754, 1838, 1979, 2033, 2387, 2669
StuI	AGGCCT	1 cut(s)	370
StyD4I	CCNGG	6 cut(s)	370, 542, 1111, 1791, 2276, 2778
StyI	CCWWGG	2 cut(s)	883, 1883
TaaI	ACNGT	8 cut(s)	297, 782, 1432, 1608, 1657, 2354, 2608, 2718
TaiI	ACGT	4 cut(s)	1649, 2499, 2535, 2757
TaqI	TCGA	7 cut(s)	264, 365, 453, 469, 2156, 2237, 2904
TaqII	GACCGA	1 cut(s)	627
TatI	WGTACW	5 cut(s)	100, 833, 1561, 1603, 2368
TauI	GCSGC	2 cut(s)	23, 2633
TfiI	GAWTC	6 cut(s)	272, 455, 634, 716, 1731, 2835
Tru1I	TTAA	8 cut(s)	465, 942, 1062, 1256, 1263, 1797, 1871, 2510
Tru9I	TTAA	8 cut(s)	465, 942, 1062, 1256, 1263, 1797, 1871, 2510
TscAI	CASTG	8 cut(s)	418, 913, 1284, 1765, 2050, 2595, 2692, 2922
TseFI	GTSAC	2 cut(s)	187, 1230
TseI	GCWGC	6 cut(s)	244, 524, 879, 1343, 1916, 2021
Tsp45I	GTSAC	2 cut(s)	187, 1230
TspGWI	ACGGA	5 cut(s)	79, 374, 2496, 2607, 2649
TspRI	CASTG	8 cut(s)	418, 913, 1284, 1765, 2050, 2595, 2692, 2922
Tth111I	GACNNNGTC	1 cut(s)	361
Van91I	CCANNNNNTGG	1 cut(s)	178
VneI	GTGCAC	1 cut(s)	2201
VpaK11BI	GGWCC	4 cut(s)	639, 1115, 2220, 2782
XceI	RCATGY	4 cut(s)	133, 380, 1923, 2727
XspI	CTAG	7 cut(s)	920, 1754, 1838, 1979, 2033, 2387, 2669
ZrmI	AGTACT	2 cut(s)	102, 1605
Zsp2I	ATGCAT	3 cut(s)	870, 1008, 2769

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.