Rroxscaffold_3G00239980

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002335
Physical Location & Seq
Forward (+)
31355744 .. 31360942
5199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239980.1

Sequence Viewer

Length: 1119 bp
ATGCTCGGCCGGGACACGTGGCGATTATTGCTGGCTTCTGTGAGGATCCGAGTCGCTGTTACGAAAGGGTGGAAAGCTAATCCTGTTGAATTTGATTCGGTGTTTAACCGGAGCCGGCATACATTTTCGTATGGTAGTCCGGACATAGTTCCGATTTTCTTTGCTGCATTGCCTCACACTACTTGGAACAGTGATCCTCATGATTTCGAAGACTTTGCTACTGGTGCCTTCTTTCTGGATGAGTGGTCGTTTGATCAATTTCAGAGCCTAATGAATAGGTCCAAAGAGGACCCAAAGTTGAAGGAGTTACTTTTCCAGGATAATCTTGTTGTGTTTTTGCACCTACTTGGTTGTGATTCAAATGGTCATGCACATCGCCCCTTTTCCTCTATCTATCTCAATAATGTCGCAGTTGTTGACAGTATAGCTGAAAGGGTTTATAATCTTCTTGAAGATTACTACAAGGACAATCGTACAGCATACGTATTTACTGCTGATCATGGAACGCATGATAAAGGTAGCCACGGAGATGGACATCCTTCAAACACCGATACTCCCCTTGTTGTTTGGGGAGCAGGTGTTAAACACCCAAAGCCTATTTCCAGCAGCAGCCATGGAGATGGACATTCTGATTGTGGTACTCGTTTTGTTGATGACCATATGCATGACACGCCAACCCCTAAAGAATGGGGCCTTCATGGCATTGAAAGGGTGGATGTCAATCAAGCTGATATCGCACCTCTCATGTCAACACTTCTTGGATTGCCATGTCCTGTAAACTCAGTTGGGAGTTTACCCCTTGATTACATAGACATGAAAAAGACAAATGAAGTTGAAGCTGTTCTAGCCAATACAAAGCAAGTTCTCAACCAATTTGTTCGGAAATCACAAACCAAGGGGGCAACTTCATTATATTTTAAGCCTTTCAAACCATTGGGCCATTATTCAACTCTCTTGGATCAGATTGAGGATCATATATCTAATAGAGACTATGAAGCTGCAAGGAAACTATCTGAAAATCTCCAAGACTTGGCACTGCAAGGACTTCATTATTTTCAAACTTATGATTGGCTGATGCTCATGACTGTAATTATTCTTGGGTATATATTAGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest PigN Sulfatase

Protein Analysis

372

Amino Acids

41.98

Weight (kDa)

5.77

Isoelectric Point (pI)

28.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfatase PF00884 130 - 202 8.3e-08 Sulfatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 441
AarI CACCTGC 1 cut(s) 566
Acc36I ACCTGC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 1030
AccIII TCCGGA 1 cut(s) 139
AclWI GGATC 5 cut(s) 40, 53, 188, 966, 978
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 1 cut(s) 89
AcvI CACGTG 1 cut(s) 18
AfaI GTAC 2 cut(s) 475, 640
AfiI CCNNNNNNNGG 1 cut(s) 1030
AflIII ACRYGT 1 cut(s) 15
AjnI CCWGG 1 cut(s) 315
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 5 cut(s) 77, 428, 728, 839, 998
AluI AGCT 5 cut(s) 77, 428, 728, 839, 998
Alw26I GTCTC 1 cut(s) 981
AlwI GGATC 5 cut(s) 40, 53, 188, 966, 978
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 3 cut(s) 7, 691, 937
ApeKI GCWGC 4 cut(s) 164, 606, 609, 998
ApoI RAATTY 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 840
AspS9I GGNCC 4 cut(s) 279, 289, 691, 937
AsuC2I CCSGG 1 cut(s) 11
AsuII TTCGAA 1 cut(s) 207
AvaII GGWCC 2 cut(s) 279, 289
BamHI GGATCC 1 cut(s) 45
BanI GGYRCC 1 cut(s) 224
BbrPI CACGTG 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 216
BbvI GCAGC 4 cut(s) 151, 618, 621, 985
BccI CCATC 2 cut(s) 524, 614
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 1 cut(s) 317
BclI TGATCA 2 cut(s) 253, 496
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 981
BfaI CTAG 1 cut(s) 845
BfuAI ACCTGC 1 cut(s) 566
BglI GCCNNNNNGGC 1 cut(s) 699
BisI GCNGC 4 cut(s) 165, 607, 610, 999
BlsI GCNGC 4 cut(s) 166, 608, 611, 1000
Bme1390I CCNGG 2 cut(s) 11, 317
Bme18I GGWCC 2 cut(s) 279, 289
BmgT120I GGNCC 4 cut(s) 279, 289, 691, 937
BmiI GGNNCC 5 cut(s) 47, 113, 226, 291, 692
BmrFI CCNGG 2 cut(s) 11, 317
BmsI GCATC 1 cut(s) 1065
BpiI GAAGAC 1 cut(s) 216
Bpu14I TTCGAA 1 cut(s) 207
BpuMI CCSGG 1 cut(s) 11
BsaAI YACGTR 2 cut(s) 18, 484
BsaBI GATNNNNATC 2 cut(s) 534, 720
BsaJI CCNNGG 3 cut(s) 523, 613, 894
BsaWI WCCGGW 2 cut(s) 108, 139
BsaXI ACNNNNNCTCC 4 cut(s) 538, 564, 568, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 1030
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 226
Bse3DI GCAATG 1 cut(s) 167
Bse8I GATNNNNATC 2 cut(s) 534, 720
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 3 cut(s) 523, 613, 894
BseGI GGATG 3 cut(s) 244, 535, 721
BseJI GATNNNNATC 2 cut(s) 534, 720
BseLI CCNNNNNNNGG 1 cut(s) 1030
BseMI GCAATG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 1 cut(s) 226
BseX3I CGGCCG 1 cut(s) 7
BseXI GCAGC 4 cut(s) 151, 618, 621, 985
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 9, 693, 939
BshNI GGYRCC 1 cut(s) 224
BsiEI CGRYCG 1 cut(s) 10
BsiSI CCGG 4 cut(s) 10, 109, 115, 140
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 1030
BsmAI GTCTC 1 cut(s) 981
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 3 cut(s) 9, 693, 939
Bsp119I TTCGAA 1 cut(s) 207
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 6 cut(s) 45, 193, 253, 496, 958, 970
Bsp19I CCATGG 1 cut(s) 613
BspANI GGCC 3 cut(s) 9, 693, 939
BspCNI CTCAG 1 cut(s) 794
BspEI TCCGGA 1 cut(s) 139
BspHI TCATGA 2 cut(s) 199, 1080
BspLI GGNNCC 5 cut(s) 47, 113, 226, 291, 692
BspMI ACCTGC 1 cut(s) 566
BspPI GGATC 5 cut(s) 40, 53, 188, 966, 978
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 3 cut(s) 523, 613, 894
BssMI GATC 6 cut(s) 45, 193, 253, 496, 958, 970
BssT1I CCWWGG 2 cut(s) 613, 894
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 3 cut(s) 191, 422, 1087
BstBAI YACGTR 2 cut(s) 18, 484
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 33, 116
BstDEI CTNAG 1 cut(s) 781
BstDSI CCRYGG 2 cut(s) 523, 613
BstF5I GGATG 3 cut(s) 244, 535, 721
BstKTI GATC 6 cut(s) 48, 196, 256, 499, 961, 973
BstMAI GTCTC 1 cut(s) 981
BstMBI GATC 6 cut(s) 45, 193, 253, 496, 958, 970
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 6 cut(s) 28, 224, 670, 699, 734, 845
BstNI CCWGG 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 9, 315
BstSNI TACGTA 1 cut(s) 484
BstV1I GCAGC 4 cut(s) 151, 618, 621, 985
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 45
BstXI CCANNNNNNTGG 2 cut(s) 530, 620
BstYI RGATCY 1 cut(s) 45
BstZI CGGCCG 1 cut(s) 7
BsuRI GGCC 3 cut(s) 9, 693, 939
BtgI CCRYGG 2 cut(s) 523, 613
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 3 cut(s) 244, 535, 721
BtsI GCAGTG 1 cut(s) 1034
BtsIMutI CAGTG 2 cut(s) 196, 1034
BveI ACCTGC 1 cut(s) 566
Cac8I GCNNGC 2 cut(s) 33, 116
CciI TCATGA 2 cut(s) 199, 1080
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 4 cut(s) 279, 289, 691, 937
Csp6I GTAC 2 cut(s) 474, 639
CviQI GTAC 2 cut(s) 474, 639
DdeI CTNAG 1 cut(s) 781
DpnI GATC 6 cut(s) 47, 195, 255, 498, 960, 972
DpnII GATC 6 cut(s) 45, 193, 253, 496, 958, 970
EaeI YGGCCR 1 cut(s) 7
EagI CGGCCG 1 cut(s) 7
EclXI CGGCCG 1 cut(s) 7
Eco105I TACGTA 1 cut(s) 484
Eco130I CCWWGG 2 cut(s) 613, 894
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 2 cut(s) 279, 289
Eco52I CGGCCG 1 cut(s) 7
Eco72I CACGTG 1 cut(s) 18
EcoO109I RGGNCCY 2 cut(s) 289, 691
EcoRII CCWGG 1 cut(s) 315
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 2 cut(s) 613, 894
EcoT22I ATGCAT 1 cut(s) 666
ErhI CCWWGG 2 cut(s) 613, 894
FaqI GGGAC 1 cut(s) 26
FauNDI CATATG 1 cut(s) 660
FbaI TGATCA 2 cut(s) 253, 496
Fnu4HI GCNGC 4 cut(s) 165, 607, 610, 999
FokI GGATG 3 cut(s) 251, 522, 728
Fsp4HI GCNGC 4 cut(s) 165, 607, 610, 999
FspBI CTAG 1 cut(s) 845
GluI GCNGC 4 cut(s) 165, 607, 610, 999
HaeIII GGCC 3 cut(s) 9, 693, 939
HapII CCGG 4 cut(s) 10, 109, 115, 140
HincII GTYRAC 2 cut(s) 418, 750
HindII GTYRAC 2 cut(s) 418, 750
HinfI GANTC 3 cut(s) 51, 95, 356
HpaII CCGG 4 cut(s) 10, 109, 115, 140
Hpy166II GTNNAC 4 cut(s) 418, 750, 778, 794
Hpy188I TCNGA 7 cut(s) 50, 153, 264, 631, 882, 963, 1015
Hpy188III TCNNGA 5 cut(s) 140, 200, 236, 449, 1081
Hpy8I GTNNAC 4 cut(s) 418, 750, 778, 794
HpyAV CCTTC 4 cut(s) 238, 295, 549, 704
HpyCH4III ACNGT 3 cut(s) 191, 422, 1087
HpyCH4IV ACGT 2 cut(s) 17, 483
HpyCH4V TGCA 6 cut(s) 167, 340, 371, 664, 1001, 1039
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 224, 670, 699, 734, 845
HpyF3I CTNAG 1 cut(s) 781
HpySE526I ACGT 2 cut(s) 17, 483
Kpn2I TCCGGA 1 cut(s) 139
KroI GCCGGC 1 cut(s) 114
KroNI GCCGGC 1 cut(s) 116
Ksp22I TGATCA 2 cut(s) 253, 496
Kzo9I GATC 6 cut(s) 45, 193, 253, 496, 958, 970
LmnI GCTCC 2 cut(s) 111, 572
Lsp1109I GCAGC 4 cut(s) 151, 618, 621, 985
LweI GCATC 1 cut(s) 1065
MaeI CTAG 1 cut(s) 845
MaeII ACGT 2 cut(s) 17, 483
MaeIII GTNAC 2 cut(s) 58, 306
MalI GATC 6 cut(s) 47, 195, 255, 498, 960, 972
MboI GATC 6 cut(s) 45, 193, 253, 496, 958, 970
MboII GAAGA 3 cut(s) 221, 437, 464
MflI RGATCY 1 cut(s) 45
MluCI AATT 4 cut(s) 89, 257, 872, 1089
MlyI GAGTC 1 cut(s) 60
MmeI TCCRAC 1 cut(s) 1093
MnlI CCTC 7 cut(s) 36, 183, 207, 280, 397, 750, 961
Mph1103I ATGCAT 1 cut(s) 666
MroI TCCGGA 1 cut(s) 139
MroNI GCCGGC 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 840
MseI TTAA 3 cut(s) 105, 582, 918
MslI CAYNNNNRTG 4 cut(s) 528, 618, 663, 812
MspI CCGG 4 cut(s) 10, 109, 115, 140
MspR9I CCNGG 2 cut(s) 11, 317
MvaI CCWGG 1 cut(s) 317
MwoI GCNNNNNNNGC 6 cut(s) 28, 224, 670, 699, 734, 845
NaeI GCCGGC 1 cut(s) 116
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 1 cut(s) 613
NdeI CATATG 1 cut(s) 660
NdeII GATC 6 cut(s) 45, 193, 253, 496, 958, 970
NgoMIV GCCGGC 1 cut(s) 114
NlaIV GGNNCC 5 cut(s) 47, 113, 226, 291, 692
NsiI ATGCAT 1 cut(s) 666
NspV TTCGAA 1 cut(s) 207
PagI TCATGA 2 cut(s) 199, 1080
PaqCI CACCTGC 1 cut(s) 566
PdiI GCCGGC 1 cut(s) 116
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 2 cut(s) 95, 356
PflMI CCANNNNNTGG 1 cut(s) 1030
PfoI TCCNGGA 1 cut(s) 315
PkrI GCNGC 4 cut(s) 166, 608, 611, 1000
PleI GAGTC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 18
PmlI CACGTG 1 cut(s) 18
PpsI GAGTC 1 cut(s) 59
Ppu21I YACGTR 2 cut(s) 18, 484
PpuMI RGGWCCY 1 cut(s) 289
PsiI TTATAA 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 315
PspCI CACGTG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 315
PspN4I GGNNCC 5 cut(s) 47, 113, 226, 291, 692
PspPI GGNCC 4 cut(s) 279, 289, 691, 937
PspPPI RGGWCCY 1 cut(s) 289
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 2 cut(s) 475, 640
RsaNI GTAC 2 cut(s) 474, 639
RseI CAYNNNNRTG 4 cut(s) 528, 618, 663, 812
SaqAI TTAA 3 cut(s) 105, 582, 918
SatI GCNGC 4 cut(s) 165, 607, 610, 999
Sau3AI GATC 6 cut(s) 45, 193, 253, 496, 958, 970
Sau96I GGNCC 4 cut(s) 279, 289, 691, 937
SchI GAGTC 1 cut(s) 60
ScrFI CCNGG 2 cut(s) 11, 317
SfaNI GCATC 1 cut(s) 1065
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 2 cut(s) 279, 289
SmiMI CAYNNNNRTG 4 cut(s) 528, 618, 663, 812
SnaBI TACGTA 1 cut(s) 484
Sse9I AATT 4 cut(s) 89, 257, 872, 1089
SspMI CTAG 1 cut(s) 845
StyD4I CCNGG 2 cut(s) 9, 315
StyI CCWWGG 2 cut(s) 613, 894
TaaI ACNGT 3 cut(s) 191, 422, 1087
TaiI ACGT 2 cut(s) 20, 486
TaqI TCGA 1 cut(s) 207
TasI AATT 4 cut(s) 89, 257, 872, 1089
TfiI GAWTC 2 cut(s) 95, 356
Tru1I TTAA 3 cut(s) 105, 582, 918
Tru9I TTAA 3 cut(s) 105, 582, 918
TscAI CASTG 2 cut(s) 196, 1041
TseI GCWGC 4 cut(s) 164, 606, 609, 998
TspDTI ATGAA 7 cut(s) 287, 686, 830, 843, 897, 1008, 1037
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 2 cut(s) 196, 1041
Van91I CCANNNNNTGG 1 cut(s) 1030
VpaK11BI GGWCC 2 cut(s) 279, 289
XapI RAATTY 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 1 cut(s) 845
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.