Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002335

Physical Location & Seq

Genomic Coordinates Reverse (-)

79517051 .. 79523351

Gene Structure 6301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021957

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2964 bp

ATGGGAGGGAGAGAGGTAGAAGAAAGCAGCAGAAGCCGGAAAGTAGTGAACTTAAGCGTGAGAAGAAAATGGCTGAAGACGAAAGAGAAATGGCTCGTCGTCGTCGGCGTCGTCCTCCACGCCGTTTACATGCTCAGCATCTTCGACATCTACTTCAAGTCCCCCATTGTTCACGGCATGGATCTTGTCTCCCCTCGCTTCCCCGCCCCCGCCAAACGCCTCGTCTTACTCGTCGCTGACGGTCTCCGAGCGGACAAGTTCTTCGAGTCCGACGCCGCCGGGAAGTTCAGAGCTCCGTTTCTGAGGAGCGTGATCAAGGAGCGAGGTCGGTGGGGAGTCTCTCATGCTCGGCCGCCGACTGAGTCGAGGCCGGGACACGTGGCGATCATTGCCGGCTTCTATGAGGATCCGAGTGCTGTTACGAAAGGGTGGAAAGCTAATCCTGTTGAATTCGATTCGGTGTTTAACCGGAGCCGGCATACATTTTCGTATGGCAGTCCGGATATAGTTCCAATTTTCTGCGCTGCTTTGCCTCACACTACTTGGAATAGTTATCCTCATGATTTCGAAGACTTTGCTACTGATGCCTCCTTTCTGGATGAGTGGTCGTTTGATCAATTTCAGAGCCTAATGAATAGGTCCAAAGAGGACCCAAAGTTGAACGAGTTACTTTTACAGGATAATCTTGTTGTGTTTTTGCACCTACTTGGTTGTGATTCAAATGGTCATGCACATCGGCCCTTTTCCTCCATCTATCTCAATAATGTCGCGGTTGTTGACAGTATTGCTGAAAGGGTTTATTATCTTCTTGAAGATTACTACAAGGACAATCGTACAGCATATGTATTTACTGCTGATCATGGAATGCATGATAAAGGTAGCCACGGAGATGGACATCCTTCAAACACTGATACTCCCCTTGTTGTTTGGGGAGCAGGCGTTAAACATCCGAAGCCTATTTCCAGCAGCAGCCATGGAGATGGACATTCTGATTGTGGTACTCGTTTTGTTGATGACCATATGCATGACACGCCAACGCCTAAAGAATGGGGCCTTCATGGCATTGAAAGGGTGGATGTCAATCAAGCTGATATTGCAACTCTCATGTCAACACTTCTTGGATTGCCATGTCCTGTAAACTCGGTTGGGAGTTTACCCCTTGATTACATAGACATGAAAAAGACAGATGAAGTTGAAGCTGTTCTAGCCAATACAAAGCAAGTTCTCAATCAATTTATTCGGAAATCACAAACGAAGGAGGCAACTTCATTATATTTTAAGCCTTTCAAACCATTGGGCCATTATTCATCCCTTTTGGGTCAGATTGAGGATCATATATCTAATGGAGACTATGAAGCTGCAAGGAAACTATCTGAAAATCTCCGAGACTTGGCACTGCAAGGACTTCACTATTTTCAAACTTATGATTGGCTGATGCTTATGACTGTAATTATTCTTGGGTATATTGGTTGGATGACCTATATTGTTCTCCATGTGTTGCAATCTTACACGTCATTGGCAGGGGACATATTGACAAAGGAGCAAGCAGATCACCGGACAGATAATACCAGAAAAGTACAACTTTGTGGATGCTTGTTTCTGGGACTACTTAGCATTATACTGTTCATGGAGCAGTCTCCTCCTCTTTACCATGCATACACAACAATGACAGTATTTTTGTGGACACAAATATCTAGTGAATATCGGTTCATAAAAGCATTATGGAAACAGTTACACGGAAGAGAAATAAATTATTTCACAAAAGTTCTAGCCGCTTGTGCTGTCTCAGTATTCATACTTGAATATCTGGTGAATAGCTTCACCGGGAGGAAGCTCTACACATGGTGTTTTCTGACTGTGGGGGTCATAGCCTTTCTTTATCTTTTCAAGTCAATTCCATGGAGATCTGGGATTCCATTCTTTGTTTGTGGTGCATGCTGGTTCTTATCTGTATTTACTTTGATGCCAGCGGAAATTCCGGATAATAATAATCTAGTAATTTGGAGTGGGGTTATGATTATTTTGATCGGAGCAGCTGGGAGGTTTCTAGATCAGCATACTGAAGGAAACAAATTTTGGCTCACTATCTGTAATAGTGATGCAAAGAAATCCAAGTTCCCCATGCTTTTTCAATTACAGGCTCTTTTGGTTGGATTATCATCTGTGATGGTATCAATATCAACATCTCATAGAACGGAGAACCAAGAACTGCATGTCTGGCACCAGATTATAAATTGGTCCATTGCCGGTCCCTCGATGGTACTTCCATTATTTTCAGCAAATGGTCTCTTGTCCCGGCTTACGTCCATATTTCTTGGCTTTGCGCCCACATTTCTTCTATTATCTATTGGATATGAAGCTGTCTTCTATGGTGCTTTTGCTCTTGTACTGATGGCTTGGATACTATTTGAAAACACACTTATTTACTCAAGTAAGGTGAAAAGGTTGTCAAGTTCCTTTAACAATATGGAGGATAATCCCATAGTTGATGGTAGATACTTGCAGCTGTCTGACATCAGAATTCCTTTAACCTTTATGGTCTTGTTTAATGTTGCCTTCTTCGGAACGGGAAATTTTGCAAGTATCGCAAGTTTCGAAATCTCGTCAGTCTATCGGTTCATTACTGTCTTCAGCCCTTTTCTTATGGCAGCGTTACTCATCTTCAAGCTATTCATTCCATTTCTGCTTGTCATATGCGTATTCAGTGCAATAACAAAGCTGAACCGACTTCCACGATTGGGATGCTATTTTCTCGTTATATTGTTTTCAGACGTTATGACCATGCATTTCTTCTTCCTGGTCCAGAATACAGGAAGTTGGATGGAAATTGGTAACAGCATCAGCCATTTTGGAATCATGAGTGCTCAAGTTGTCTTTGTTCTGTTGCTTTTCGCACTCACAAACGTATACACGAAGGATATCTGTATTGGTTCTGTGGAGCGGTCTTCTCGGAAAGCCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 map00563 map01100

Pfam Domains

Protein Families

Phosphodiest PigN Sulfatase

Protein Analysis

988

Amino Acids

111.58

Weight (kDa)

7.4

Isoelectric Point (pI)

36.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sulfatase	PF00884	248 - 322	5.4e-06	Sulfatase
PigN	PF04987	467 - 939	1.3e-126	Phosphatidylinositolglycan class N (PIG-N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002335)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01380 AT3G01380
fragaria_vesca	FvH4_6g50180 FvH4_6g50180 FvH4_6g50180
malus_domestica	MD09G1038400.v1.1 MD17G1039500.v1.1
prunus_persica	Prupe.2G019300_v2.0.a1 Prupe.3G279400_v2.0.a1 Prupe.3G279400_v2.0.a1
pyrus_communis	pycom111g03060 pycom17g03550 pycom17g03560
rosa_chinensis	RchiOBHm_Chr2g0170441 RchiOBHm_Chr7g0219531 RchiOBHm_Chr7g0219551 RchiOBHm_Chr7g0219561 RchiOBHm_Chr7g0219571 RchiOBHm_Chr7g0219581
rosa_laevigata	RLG00000002284 RLG00000021957
rosa_multiflora	Rmu_co7962372.1_g000001 Rmu_sc0009395.1_g000013
rosa_roxburghii	Rroxscaffold_2G00081260 Rroxscaffold_2G00081270 Rroxscaffold_3G00239980
rosa_rugosa	Rorug02G0548900 Rorug07G0186100 Rorug07G0186200 Rorug07G0186300
rosa_samantha	Rh2AG620800 Rh2BG631800 Rh2CG601400 Rh2DG644500 Rh7AG330000 Rh7AG330100 Rh7BG319700 Rh7BG319800 Rh7CG347300 Rh7CG347400
rosa_wichuraiana	Rw2G051460 Rw7G027790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2231
AccB1I	GGYRCC	1 cut(s)	2220
AccBSI	CCGCTC	2 cut(s)	251, 2941
AccI	GTMKAC	1 cut(s)	2907
AccII	CGCG	1 cut(s)	770
AccIII	TCCGGA	2 cut(s)	499, 1978
AciI	CCGC	9 cut(s)	204, 210, 251, 276, 353, 770, 1773, 1970, 2941
AclWI	GGATC	4 cut(s)	189, 401, 414, 1338
AcoI	YGGCCR	1 cut(s)	350
AcsI	RAATTY	5 cut(s)	449, 1974, 2072, 2520, 2572
AcuI	CTGAAG	3 cut(s)	95, 2082, 2614
AcvI	CACGTG	1 cut(s)	379
AcyI	GRCGYC	2 cut(s)	108, 273
AdeI	CACNNNGTG	1 cut(s)	1845
AfaI	GTAC	5 cut(s)	835, 1000, 1578, 2262, 2388
AfiI	CCNNNNNNNGG	2 cut(s)	1390, 2738
AflII	CTTAAG	1 cut(s)	52
AflIII	ACRYGT	2 cut(s)	376, 1509
AjiI	CACGTC	1 cut(s)	1512
AjnI	CCWGG	1 cut(s)	2796
AjuI	GAANNNNNNNTTGG	2 cut(s)	2059, 2091
Alw21I	GWGCWC	2 cut(s)	295, 2866
Alw26I	GTCTC	8 cut(s)	193, 248, 343, 1341, 1380, 1641, 1789, 2291
AlwI	GGATC	4 cut(s)	189, 401, 414, 1338
Aor13HI	TCCGGA	2 cut(s)	499, 1978
AoxI	GGCC	5 cut(s)	350, 368, 737, 1051, 1297
ApeKI	GCWGC	8 cut(s)	27, 524, 966, 969, 1358, 2033, 2503, 2648
ApoI	RAATTY	5 cut(s)	449, 1974, 2072, 2520, 2572
ArsI	GACNNNNNNTTYG	6 cut(s)	207, 239, 245, 277, 1660, 1692
Asp700I	GAANNNNTTC	2 cut(s)	1200, 1817
AspLEI	GCGC	2 cut(s)	524, 2326
AspS9I	GGNCC	8 cut(s)	639, 649, 738, 1051, 1297, 2238, 2249, 2800
AsuC2I	CCSGG	4 cut(s)	280, 372, 1825, 2296
AsuHPI	GGTGA	4 cut(s)	1544, 1813, 1822, 2449
AsuII	TTCGAA	2 cut(s)	567, 2595
AvaII	GGWCC	5 cut(s)	639, 649, 2238, 2249, 2800
BamHI	GGATCC	1 cut(s)	406
BanI	GGYRCC	1 cut(s)	2220
BanII	GRGCYC	1 cut(s)	295
BbrPI	CACGTG	1 cut(s)	379
BbsI	GAAGAC	5 cut(s)	83, 576, 2356, 2620, 2937
Bbv12I	GWGCWC	2 cut(s)	295, 2866
BbvI	GCAGC	8 cut(s)	39, 511, 978, 981, 1345, 2045, 2515, 2660
BccI	CCATC	8 cut(s)	758, 884, 974, 2161, 2251, 2386, 2483, 2815
BceAI	ACGGC	2 cut(s)	107, 190
BcgI	CGANNNNNNTGC	4 cut(s)	557, 591, 2724, 2758
BciT130I	CCWGG	1 cut(s)	2798
BciVI	GTATCC	1 cut(s)	2394
BclI	TGATCA	3 cut(s)	312, 613, 856
BcnI	CCSGG	4 cut(s)	280, 372, 1825, 2296
BcoDI	GTCTC	8 cut(s)	193, 248, 343, 1341, 1380, 1641, 1789, 2291
BfaI	CTAG	5 cut(s)	1205, 1695, 1769, 1994, 2048
BfrI	CTTAAG	1 cut(s)	52
BfuI	GTATCC	1 cut(s)	2394
BglI	GCCNNNNNGGC	1 cut(s)	1059
BglII	AGATCT	1 cut(s)	1904
BlpI	GCTNAGC	1 cut(s)	134
Bme1390I	CCNGG	5 cut(s)	280, 372, 1825, 2296, 2798
Bme18I	GGWCC	5 cut(s)	639, 649, 2238, 2249, 2800
BmgBI	CACGTC	1 cut(s)	1512
BmgT120I	GGNCC	8 cut(s)	639, 649, 738, 1051, 1297, 2238, 2249, 2800
BmiI	GGNNCC	6 cut(s)	408, 473, 651, 1052, 2222, 2251
BmrFI	CCNGG	5 cut(s)	280, 372, 1825, 2296, 2798
BmsI	GCATC	8 cut(s)	147, 574, 1425, 1580, 1953, 2089, 2732, 2847
BpiI	GAAGAC	5 cut(s)	83, 576, 2356, 2620, 2937
Bpu1102I	GCTNAGC	1 cut(s)	134
Bpu14I	TTCGAA	2 cut(s)	567, 2595
BpuEI	CTTGAG	2 cut(s)	2413, 2850
BpuMI	CCSGG	4 cut(s)	280, 372, 1825, 2296
BsaAI	YACGTR	1 cut(s)	379
BsaBI	GATNNNNATC	3 cut(s)	894, 1080, 2158
BsaHI	GRCGYC	2 cut(s)	108, 273
BsaI	GGTCTC	2 cut(s)	248, 2291
BsaJI	CCNNGG	3 cut(s)	883, 973, 1898
BsaWI	WCCGGW	4 cut(s)	468, 499, 1554, 1978
BsaXI	ACNNNNNCTCC	4 cut(s)	898, 924, 928, 954
Bsc4I	CCNNNNNNNGG	2 cut(s)	1390, 2738
Bse118I	RCCGGY	3 cut(s)	392, 474, 2246
Bse3DI	GCAATG	2 cut(s)	387, 2241
Bse8I	GATNNNNATC	3 cut(s)	894, 1080, 2158
BseAI	TCCGGA	2 cut(s)	499, 1978
BseBI	CCWGG	1 cut(s)	2798
BseDI	CCNNGG	3 cut(s)	883, 973, 1898
BseGI	GGATG	9 cut(s)	604, 895, 946, 1081, 1307, 1479, 1595, 2747, 2826
BseJI	GATNNNNATC	3 cut(s)	894, 1080, 2158
BseLI	CCNNNNNNNGG	2 cut(s)	1390, 2738
BseMI	GCAATG	2 cut(s)	387, 2241
BseMII	CTCAG	4 cut(s)	148, 293, 351, 1800
BseRI	GAGGAG	3 cut(s)	319, 1629, 1632
BseX3I	CGGCCG	1 cut(s)	350
BseXI	GCAGC	8 cut(s)	39, 511, 978, 981, 1345, 2045, 2515, 2660
BseYI	CCCAGC	1 cut(s)	2036
Bsh1236I	CGCG	1 cut(s)	770
Bsh1285I	CGRYCG	1 cut(s)	353
BshFI	GGCC	5 cut(s)	352, 370, 739, 1053, 1299
BshNI	GGYRCC	1 cut(s)	2220
BsiEI	CGRYCG	1 cut(s)	353
BsiHKAI	GWGCWC	2 cut(s)	295, 2866
BslFI	GGGAC	6 cut(s)	145, 387, 1538, 1617, 2235, 2278
BslI	CCNNNNNNNGG	2 cut(s)	1390, 2738
BsmAI	GTCTC	8 cut(s)	193, 248, 343, 1341, 1380, 1641, 1789, 2291
BsmFI	GGGAC	6 cut(s)	145, 387, 1538, 1617, 2235, 2278
BsmI	GAATGC	1 cut(s)	870
BsnI	GGCC	5 cut(s)	352, 370, 739, 1053, 1299
Bso31I	GGTCTC	2 cut(s)	248, 2291
Bsp119I	TTCGAA	2 cut(s)	567, 2595
Bsp1286I	GDGCHC	2 cut(s)	295, 2866
Bsp13I	TCCGGA	2 cut(s)	499, 1978
Bsp1720I	GCTNAGC	1 cut(s)	134
Bsp19I	CCATGG	2 cut(s)	973, 1898
BspACI	CCGC	9 cut(s)	204, 210, 251, 276, 353, 770, 1773, 1970, 2941
BspANI	GGCC	5 cut(s)	352, 370, 739, 1053, 1299
BspCNI	CTCAG	4 cut(s)	147, 294, 352, 1799
BspEI	TCCGGA	2 cut(s)	499, 1978
BspFNI	CGCG	1 cut(s)	770
BspHI	TCATGA	2 cut(s)	559, 2856
BspLI	GGNNCC	6 cut(s)	408, 473, 651, 1052, 2222, 2251
BspPI	GGATC	4 cut(s)	189, 401, 414, 1338
BspT104I	TTCGAA	2 cut(s)	567, 2595
BspT107I	GGYRCC	1 cut(s)	2220
BspTI	CTTAAG	1 cut(s)	52
BspTNI	GGTCTC	2 cut(s)	248, 2291
BsrBI	CCGCTC	2 cut(s)	251, 2941
BsrDI	GCAATG	2 cut(s)	387, 2241
BsrFI	RCCGGY	3 cut(s)	392, 474, 2246
BssAI	RCCGGY	3 cut(s)	392, 474, 2246
BssECI	CCNNGG	3 cut(s)	883, 973, 1898
BssNAI	GTATAC	1 cut(s)	2908
BssNI	GRCGYC	2 cut(s)	108, 273
BssT1I	CCWWGG	2 cut(s)	973, 1898
Bst1107I	GTATAC	1 cut(s)	2908
Bst2UI	CCWGG	1 cut(s)	2798
Bst4CI	ACNGT	8 cut(s)	242, 782, 1447, 1623, 1672, 1731, 1858, 2626
Bst6I	CTCTTC	1 cut(s)	1735
BstACI	GRCGYC	2 cut(s)	108, 273
BstAFI	CTTAAG	1 cut(s)	52
BstBAI	YACGTR	1 cut(s)	379
BstBI	TTCGAA	2 cut(s)	567, 2595
BstC8I	GCNNGC	6 cut(s)	394, 476, 937, 1545, 1936, 1968
BstDEI	CTNAG	5 cut(s)	134, 302, 360, 1610, 1786
BstDSI	CCRYGG	3 cut(s)	883, 973, 1898
BstF5I	GGATG	9 cut(s)	604, 895, 946, 1081, 1307, 1479, 1595, 2747, 2826
BstFNI	CGCG	1 cut(s)	770
BstHHI	GCGC	2 cut(s)	524, 2326
BstMAI	GTCTC	8 cut(s)	193, 248, 343, 1341, 1380, 1641, 1789, 2291
BstMCI	CGRYCG	1 cut(s)	353
BstNI	CCWGG	1 cut(s)	2798
BstNSI	RCATGY	3 cut(s)	133, 1938, 2216
BstSCI	CCNGG	5 cut(s)	278, 370, 1823, 2294, 2796
BstUI	CGCG	1 cut(s)	770
BstV1I	GCAGC	8 cut(s)	39, 511, 978, 981, 1345, 2045, 2515, 2660
BstV2I	GAAGAC	5 cut(s)	83, 576, 2356, 2620, 2937
BstX2I	RGATCY	3 cut(s)	181, 406, 1904
BstXI	CCANNNNNNTGG	2 cut(s)	890, 980
BstYI	RGATCY	3 cut(s)	181, 406, 1904
BstZ17I	GTATAC	1 cut(s)	2908
BstZI	CGGCCG	1 cut(s)	350
BsuI	GTATCC	1 cut(s)	2394
BsuRI	GGCC	5 cut(s)	352, 370, 739, 1053, 1299
BtgI	CCRYGG	3 cut(s)	883, 973, 1898
BtrI	CACGTC	1 cut(s)	1512
BtsCI	GGATG	9 cut(s)	604, 895, 946, 1081, 1307, 1479, 1595, 2747, 2826
BtsI	GCAGTG	1 cut(s)	1394
BtsIMutI	CAGTG	3 cut(s)	906, 1394, 2710
Cac8I	GCNNGC	6 cut(s)	394, 476, 937, 1545, 1936, 1968
CciI	TCATGA	2 cut(s)	559, 2856
CfoI	GCGC	2 cut(s)	524, 2326
Cfr10I	RCCGGY	3 cut(s)	392, 474, 2246
Cfr13I	GGNCC	8 cut(s)	639, 649, 738, 1051, 1297, 2238, 2249, 2800
CseI	GACGC	2 cut(s)	97, 281
Csp6I	GTAC	5 cut(s)	834, 999, 1577, 2261, 2387
CviQI	GTAC	5 cut(s)	834, 999, 1577, 2261, 2387
DdeI	CTNAG	5 cut(s)	134, 302, 360, 1610, 1786
DraIII	CACNNNGTG	1 cut(s)	1845
EaeI	YGGCCR	1 cut(s)	350
EagI	CGGCCG	1 cut(s)	350
Eam1104I	CTCTTC	1 cut(s)	1735
EarI	CTCTTC	1 cut(s)	1735
Ecl136II	GAGCTC	1 cut(s)	293
EclXI	CGGCCG	1 cut(s)	350
Eco130I	CCWWGG	2 cut(s)	973, 1898
Eco24I	GRGCYC	1 cut(s)	295
Eco31I	GGTCTC	2 cut(s)	248, 2291
Eco32I	GATATC	1 cut(s)	2920
Eco47I	GGWCC	5 cut(s)	639, 649, 2238, 2249, 2800
Eco52I	CGGCCG	1 cut(s)	350
Eco53kI	GAGCTC	1 cut(s)	293
Eco57I	CTGAAG	3 cut(s)	95, 2082, 2614
Eco72I	CACGTG	1 cut(s)	379
EcoICRI	GAGCTC	1 cut(s)	293
EcoO109I	RGGNCCY	2 cut(s)	649, 1051
EcoRI	GAATTC	2 cut(s)	449, 2520
EcoRII	CCWGG	1 cut(s)	2796
EcoRV	GATATC	1 cut(s)	2920
EcoT14I	CCWWGG	2 cut(s)	973, 1898
EcoT22I	ATGCAT	4 cut(s)	870, 1026, 1657, 2787
EcoT38I	GRGCYC	1 cut(s)	295
ErhI	CCWWGG	2 cut(s)	973, 1898
FalI	AAGNNNNNCTT	2 cut(s)	1566, 1598
FaqI	GGGAC	6 cut(s)	145, 387, 1538, 1617, 2235, 2278
FauI	CCCGC	2 cut(s)	211, 217
FauNDI	CATATG	3 cut(s)	841, 1020, 2693
FbaI	TGATCA	3 cut(s)	312, 613, 856
FblI	GTMKAC	1 cut(s)	2907
FokI	GGATG	9 cut(s)	611, 882, 933, 1088, 1294, 1486, 1602, 2754, 2833
FriOI	GRGCYC	1 cut(s)	295
FspBI	CTAG	5 cut(s)	1205, 1695, 1769, 1994, 2048
GlaI	GCGC	2 cut(s)	523, 2325
GsaI	CCCAGC	1 cut(s)	2040
HaeIII	GGCC	5 cut(s)	352, 370, 739, 1053, 1299
HgaI	GACGC	2 cut(s)	97, 281
HhaI	GCGC	2 cut(s)	524, 2326
Hin1I	GRCGYC	2 cut(s)	108, 273
Hin6I	GCGC	2 cut(s)	522, 2324
HinP1I	GCGC	2 cut(s)	522, 2324
HincII	GTYRAC	2 cut(s)	778, 1110
HindII	GTYRAC	2 cut(s)	778, 1110
HinfI	GANTC	7 cut(s)	266, 336, 362, 455, 716, 1912, 2853
HphI	GGTGA	4 cut(s)	1544, 1813, 1822, 2449
Hpy166II	GTNNAC	9 cut(s)	49, 127, 172, 778, 1110, 1138, 1154, 1683, 2908
Hpy188III	TCNNGA	8 cut(s)	500, 560, 596, 809, 1979, 2048, 2803, 2857
Hpy8I	GTNNAC	9 cut(s)	49, 127, 172, 778, 1110, 1138, 1154, 1683, 2908
Hpy99I	CGWCG	6 cut(s)	101, 104, 107, 113, 236, 275
HpyAV	CCTTC	6 cut(s)	909, 1064, 1249, 2057, 2566, 2908
HpyCH4III	ACNGT	8 cut(s)	242, 782, 1447, 1623, 1672, 1731, 1858, 2626
HpyCH4IV	ACGT	5 cut(s)	378, 1511, 2303, 2772, 2904
HpyF3I	CTNAG	5 cut(s)	134, 302, 360, 1610, 1786
HpySE526I	ACGT	5 cut(s)	378, 1511, 2303, 2772, 2904
Hsp92I	GRCGYC	2 cut(s)	108, 273
HspAI	GCGC	2 cut(s)	522, 2324
Kpn2I	TCCGGA	2 cut(s)	499, 1978
KroI	GCCGGC	2 cut(s)	392, 474
KroNI	GCCGGC	2 cut(s)	394, 476
Ksp22I	TGATCA	3 cut(s)	312, 613, 856
LmnI	GCTCC	9 cut(s)	298, 306, 319, 471, 932, 1540, 1630, 2030, 2938
Lsp1109I	GCAGC	8 cut(s)	39, 511, 978, 981, 1345, 2045, 2515, 2660
LweI	GCATC	8 cut(s)	147, 574, 1425, 1580, 1953, 2089, 2732, 2847
MaeI	CTAG	5 cut(s)	1205, 1695, 1769, 1994, 2048
MaeII	ACGT	5 cut(s)	378, 1511, 2303, 2772, 2904
MaeIII	GTNAC	5 cut(s)	418, 666, 1731, 2652, 2831
MbiI	CCGCTC	2 cut(s)	251, 2941
MflI	RGATCY	3 cut(s)	181, 406, 1904
MhlI	GDGCHC	2 cut(s)	295, 2866
MlyI	GAGTC	3 cut(s)	275, 345, 371
MmeI	TCCRAC	4 cut(s)	294, 1451, 2131, 2798
Mph1103I	ATGCAT	4 cut(s)	870, 1026, 1657, 2787
MroI	TCCGGA	2 cut(s)	499, 1978
MroNI	GCCGGC	2 cut(s)	392, 474
MroXI	GAANNNNTTC	2 cut(s)	1200, 1817
MseI	TTAA	8 cut(s)	53, 465, 942, 1278, 2460, 2528, 2547, 2962
MslI	CAYNNNNRTG	5 cut(s)	888, 978, 1023, 1172, 1664
MspA1I	CMGCKG	3 cut(s)	1970, 2036, 2506
MspCI	CTTAAG	1 cut(s)	52
MspR9I	CCNGG	5 cut(s)	280, 372, 1825, 2296, 2798
Mva1269I	GAATGC	1 cut(s)	870
MvaI	CCWGG	1 cut(s)	2798
MvnI	CGCG	1 cut(s)	770
NaeI	GCCGGC	2 cut(s)	394, 476
NciI	CCSGG	4 cut(s)	280, 372, 1825, 2296
NcoI	CCATGG	2 cut(s)	973, 1898
NdeI	CATATG	3 cut(s)	841, 1020, 2693
NgoMIV	GCCGGC	2 cut(s)	392, 474
NlaIV	GGNNCC	6 cut(s)	408, 473, 651, 1052, 2222, 2251
NmeAIII	GCCGAG	1 cut(s)	328
NsiI	ATGCAT	4 cut(s)	870, 1026, 1657, 2787
NspI	RCATGY	3 cut(s)	133, 1938, 2216
NspV	TTCGAA	2 cut(s)	567, 2595
PaeI	GCATGC	1 cut(s)	1938
PagI	TCATGA	2 cut(s)	559, 2856
PcsI	WCGNNNNNNNCGW	4 cut(s)	105, 108, 228, 2592
PctI	GAATGC	1 cut(s)	870
PdiI	GCCGGC	2 cut(s)	394, 476
PdmI	GAANNNNTTC	2 cut(s)	1200, 1817
PfeI	GAWTC	4 cut(s)	455, 716, 1912, 2853
PflFI	GACNNNGTC	1 cut(s)	361
PleI	GAGTC	3 cut(s)	274, 344, 370
PmaCI	CACGTG	1 cut(s)	379
PmlI	CACGTG	1 cut(s)	379
PpsI	GAGTC	3 cut(s)	274, 344, 370
Ppu21I	YACGTR	1 cut(s)	379
PpuMI	RGGWCCY	1 cut(s)	649
PsiI	TTATAA	1 cut(s)	2231
Psp124BI	GAGCTC	1 cut(s)	295
Psp5II	RGGWCCY	1 cut(s)	649
Psp6I	CCWGG	1 cut(s)	2796
PspCI	CACGTG	1 cut(s)	379
PspFI	CCCAGC	1 cut(s)	2036
PspGI	CCWGG	1 cut(s)	2796
PspN4I	GGNNCC	6 cut(s)	408, 473, 651, 1052, 2222, 2251
PspPI	GGNCC	8 cut(s)	639, 649, 738, 1051, 1297, 2238, 2249, 2800
PspPPI	RGGWCCY	1 cut(s)	649
PsuI	RGATCY	3 cut(s)	181, 406, 1904
PsyI	GACNNNGTC	1 cut(s)	361
PvuII	CAGCTG	2 cut(s)	2036, 2506
RsaI	GTAC	5 cut(s)	835, 1000, 1578, 2262, 2388
RsaNI	GTAC	5 cut(s)	834, 999, 1577, 2261, 2387
RseI	CAYNNNNRTG	5 cut(s)	888, 978, 1023, 1172, 1664
SacI	GAGCTC	1 cut(s)	295
SaqAI	TTAA	8 cut(s)	53, 465, 942, 1278, 2460, 2528, 2547, 2962
Sau96I	GGNCC	8 cut(s)	639, 649, 738, 1051, 1297, 2238, 2249, 2800
SchI	GAGTC	3 cut(s)	275, 345, 371
ScrFI	CCNGG	5 cut(s)	280, 372, 1825, 2296, 2798
SduI	GDGCHC	2 cut(s)	295, 2866
SfaNI	GCATC	8 cut(s)	147, 574, 1425, 1580, 1953, 2089, 2732, 2847
SfuI	TTCGAA	2 cut(s)	567, 2595
SinI	GGWCC	5 cut(s)	639, 649, 2238, 2249, 2800
SmiMI	CAYNNNNRTG	5 cut(s)	888, 978, 1023, 1172, 1664
SmlI	CTYRAG	3 cut(s)	52, 2428, 2865
SmoI	CTYRAG	3 cut(s)	52, 2428, 2865
SphI	GCATGC	1 cut(s)	1938
SsiI	CCGC	9 cut(s)	204, 210, 251, 276, 353, 770, 1773, 1970, 2941
SspMI	CTAG	5 cut(s)	1205, 1695, 1769, 1994, 2048
SstI	GAGCTC	1 cut(s)	295
StyD4I	CCNGG	5 cut(s)	278, 370, 1823, 2294, 2796
StyI	CCWWGG	2 cut(s)	973, 1898
TaaI	ACNGT	8 cut(s)	242, 782, 1447, 1623, 1672, 1731, 1858, 2626
TaiI	ACGT	5 cut(s)	381, 1514, 2306, 2775, 2907
TaqI	TCGA	7 cut(s)	144, 264, 365, 453, 567, 2255, 2595
TatI	WGTACW	2 cut(s)	1576, 2386
TauI	GCSGC	3 cut(s)	278, 355, 1775
TfiI	GAWTC	4 cut(s)	455, 716, 1912, 2853
Tru1I	TTAA	8 cut(s)	53, 465, 942, 1278, 2460, 2528, 2547, 2962
Tru9I	TTAA	8 cut(s)	53, 465, 942, 1278, 2460, 2528, 2547, 2962
TscAI	CASTG	3 cut(s)	913, 1401, 2710
TseI	GCWGC	8 cut(s)	27, 524, 966, 969, 1358, 2033, 2503, 2648
TspGWI	ACGGA	4 cut(s)	285, 900, 1752, 2210
TspRI	CASTG	3 cut(s)	913, 1401, 2710
Tth111I	GACNNNGTC	1 cut(s)	361
Vha464I	CTTAAG	1 cut(s)	52
VpaK11BI	GGWCC	5 cut(s)	639, 649, 2238, 2249, 2800
XapI	RAATTY	5 cut(s)	449, 1974, 2072, 2520, 2572
XbaI	TCTAGA	1 cut(s)	2047
XceI	RCATGY	3 cut(s)	133, 1938, 2216
XmiI	GTMKAC	1 cut(s)	2907
XmnI	GAANNNNTTC	2 cut(s)	1200, 1817
XspI	CTAG	5 cut(s)	1205, 1695, 1769, 1994, 2048
Zsp2I	ATGCAT	4 cut(s)	870, 1026, 1657, 2787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.