Rh2CG601400

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002335
Physical Location & Seq
Reverse (-)
77682799 .. 77689115
6317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG601400.1

Sequence Viewer

Length: 2964 bp
ATGGGAGGGAAAGAGGTAGAAGAAAGCAGCAGAAGCCGGAAAGTAGTGAACTTGAGCGTGAGAAGAAAATGGCTGAAGACGAAAGAGAAATGGCTCGTCGTCGTCGGCGTCGTTCTTCACGCCGTTTACATGCTCAGCATCTTCGACATCTACTTCAAGTCCCCCGTTGTTCACGGCATGGATCTCGTCTCCCCTCGCTTCCCCGCCCCCGCCAAACGCCTCGTCTTACTCGTCGCTGATGGTCTCCGAGCGGACAAGTTCTTCGAGTCCGACGCCGCCGGAAAGTTCAGAGCGCCGTTTCTGAGGAGCGTGATCAAGGAGCGAGGTCGGTGGGGAGTCTCTCATGCTCGGCCGCCGACTGAGTCGAGGCCGGGACACGTGGCGATTATTGCTGGCTTCTATGAGGATCCGAGTGCTGTTACGAAAGGGTGGAAAGCTAATCCTGTTGAATTCGATTCGGTGTTTAATCGGAGCCGGCATACATTTTCCTATGGTAGTCCGGATATAGTTCCGATTTTCTGTGCTGCATTGCCTCACACTACTTGGAACAGTTATCCTCATGATTTTGAAGACTTTGCTACTGATGCCTCCTTTCTGGATGAGTGGTCTTTTGATCAATTTCAGAGCCTAATGAATAGGTCCAAAGAGGACCCAAAGTTGAAGGAGTTACTTTTACAGGATAATCTTGTTGTGTTTTTGCACCTACTTGGTTGTGATTCAAATGGTCATGCACATCGGCCCTTTTCCTCCATCTATCTCAATAATGTTGCGGTTGTTGACAGTATTGCTGAAAGGGTTTATTATCTTCTTGAAGATTACTACAAGGACAATCGTACAGCATATGTATTTACTGCTGATCATGGAATGCATGATAAAGGTAGCCACGGAGATGGACATCCTTCAAACACGGATACTCCCCTTGTTGTTTGGGGAGCAGGTGTTAAACATCTGAAGCCTATTTCCAGCAGCAGCCATGGAGATGGACATTCTGATTGTGGTACTCGTTTTGTTGATGACCATATGCATGACACGCCAACGCCTAAAGAATGGGGCCTTCATGGCATTGAAAGGGTGGATGTCAATCAAGCTGATATCGCACCTCTCATGTCAACACTTCTTGGATTGCCATGTCCTGTAAACTCGGTTGGGAGTTTACCCCTTGATTACATAGACATGAAAAAGACAGATGAAGTTGAAGCTGTTCTAGCCAATACAAAGCAAGTTCTCAATCAATTTATTCGGAAATCACAAACGAAGGAGGCAACTTCATTATATTTTAAGCCTTTCAAACCATTGGGCCATTATTCATCCCTCTTGGATCAGATTGAGGATCATATATCTAATGGAGACTATGAAGCTGCAAGGAAACTATCTGAAAATCTTCGAGACTTGGCACTGCAAGGACTTCACTATTTTCAAACTTATGATTGGCTGATGCTCATGACTGTAATTATTCTTGGGTATATTGGTTGGATGACCTATATTGTTCTCCATGTGTTGCAATCTTACACGTCATTGGCAGGGGACATATTGACAAAGGAGCAAGCAGATCACCGGACAGATAACACAAGAAAAGTTCAACTTTGTGGATGCTTGTTTCTGGGATTACTCAGCATTATACTGTTCATGGAGCAGTCTCCTCCTCTTTACCATGCATACACAACAATGACAGTATTTTTGTGGACACAAATATCTAGTGAATATCGGTTCATAAAAGCATTATGGAAACAGTTACACGGAAGAGAAATAAATTATTTCACAAAAGTTCTAGCCGCTTGTGCTGTCTCAGTATTCATACTTGAATATCTGGTGAATAGCTTCACCGGGAGGAAGCTCTACACATGGTGTTTTCTGACTGTGGGGGTCATAGCCTTTCTTTATCTTTTCAAGTCAATTCCATGGAGATCTGGGATTCCATTCTTTGTTTGTGGTGCATGCTGGTTCTTATCTGTATTTACTTTGATGCCAGCGGAAATTCCGGATAATAATAATCTAGTAATTTGGAGTGGAGTTATGATTATTTTGATCGGAGCAGCTGGGAGGTTTCTAGATCAGCATACTGAAGGAAACAAATTTTGGCTCACTATCTGTAATAGTGATGCAAAGAAATCCAAGTTCCCCATGCTCTTTCAATTACAGGCTCTTTTGGTTGGATTATCATCTGTGATGGTATCAATATCAACATCTCATAGAACGGAGAACCAAGAACTGCATGTCTGGCACCAGATTATAAATTGGTCCATTGCCGGTCCCTCAATGGTACTTCCATTATTTTCAGCAAATGGTCTCTTGTCCCGGCTTACGTCCATATTTCTTGGCTTTGCGCCCACATTTCTTCTATTATCTATCGGATATGAAGCTGTCTTCTATGGTGCTTTTGCTCTTGTACTCATGGCTTGGATACTATTTGAAAACACACTTATTTACTCAAGTAAGGTGAAAAGGTTGTCAAGTTCCTTTAACAATATGGAGGATAATCCCATAGTTGATGGTAGATACTTGCAGCTGTCTGACATCAGAATTCCTTTAACCTTTATGGTCTTGTTTAATGTTGCCTTCTTCGGAACGGGAAATTTTGCAAGTATCGCAAGTTTCGAAATCTCGTCAGTCTATCGGTTCATTACTGTCTTCAGCCCTTTTCTTATGGCAGCGTTACTCATCTTCAAGCTATTCATTCCATTTCTGCTTGTCATATGCGTATTCAGTGCAATAACAAAGCTGAACCGACTTCCACGATTGGGATGCTATTTTCTCGTTATATTGTTTTCAGATGTTATGACTATGCATTTCTTCTTCCTGGTCCAGAATACAGGAAGTTGGATGGAAATTGGTAACAGTATCAGCCATTTTGGAATCATGAGTGCTCAAGTTGTCTTTGTTCTGTTGCTTTTCGCACTCACAAATGTATACACGAAGGATATCTGTATTGGTTCTGTGGAGCGGTCTTCTCGGAAAGCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest PigN Sulfatase

Protein Analysis

987

Amino Acids

111.63

Weight (kDa)

7.4

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PigN PF04987 467 - 939 1.3e-126 Phosphatidylinositolglycan class N (PIG-N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2231
AarI CACCTGC 1 cut(s) 926
Acc36I ACCTGC 1 cut(s) 926
AccB1I GGYRCC 1 cut(s) 2220
AccBSI CCGCTC 2 cut(s) 251, 2941
AccI GTMKAC 1 cut(s) 2907
AccIII TCCGGA 2 cut(s) 499, 1978
AciI CCGC 9 cut(s) 204, 210, 251, 276, 353, 770, 1773, 1970, 2941
AclWI GGATC 5 cut(s) 189, 401, 414, 1326, 1338
AcoI YGGCCR 1 cut(s) 350
AcsI RAATTY 5 cut(s) 449, 1974, 2072, 2520, 2572
AcuI CTGAAG 4 cut(s) 95, 971, 2082, 2614
AcvI CACGTG 1 cut(s) 379
AcyI GRCGYC 2 cut(s) 108, 273
AdeI CACNNNGTG 1 cut(s) 1845
AfaI GTAC 4 cut(s) 835, 1000, 2262, 2388
AfiI CCNNNNNNNGG 1 cut(s) 2738
AflIII ACRYGT 2 cut(s) 376, 1509
AjiI CACGTC 1 cut(s) 1512
AjnI CCWGG 1 cut(s) 2796
AjuI GAANNNNNNNTTGG 2 cut(s) 2059, 2091
Alw21I GWGCWC 1 cut(s) 2866
Alw26I GTCTC 8 cut(s) 193, 248, 343, 1341, 1380, 1641, 1789, 2291
AlwI GGATC 5 cut(s) 189, 401, 414, 1326, 1338
Aor13HI TCCGGA 2 cut(s) 499, 1978
AoxI GGCC 5 cut(s) 350, 368, 737, 1051, 1297
ApeKI GCWGC 8 cut(s) 27, 524, 966, 969, 1358, 2033, 2503, 2648
ApoI RAATTY 5 cut(s) 449, 1974, 2072, 2520, 2572
ArsI GACNNNNNNTTYG 6 cut(s) 207, 239, 245, 277, 1660, 1692
Asp700I GAANNNNTTC 3 cut(s) 1200, 1380, 1817
AspLEI GCGC 2 cut(s) 295, 2326
AspS9I GGNCC 8 cut(s) 639, 649, 738, 1051, 1297, 2238, 2249, 2800
AsuC2I CCSGG 3 cut(s) 372, 1825, 2296
AsuHPI GGTGA 4 cut(s) 1544, 1813, 1822, 2449
AsuII TTCGAA 1 cut(s) 2595
AvaII GGWCC 5 cut(s) 639, 649, 2238, 2249, 2800
BamHI GGATCC 1 cut(s) 406
BanI GGYRCC 1 cut(s) 2220
BbrPI CACGTG 1 cut(s) 379
BbsI GAAGAC 5 cut(s) 83, 576, 2356, 2620, 2937
Bbv12I GWGCWC 1 cut(s) 2866
BbvI GCAGC 8 cut(s) 39, 511, 978, 981, 1345, 2045, 2515, 2660
BccI CCATC 7 cut(s) 233, 758, 884, 974, 2161, 2483, 2815
BceAI ACGGC 3 cut(s) 107, 190, 280
BcgI CGANNNNNNTGC 4 cut(s) 166, 200, 2724, 2758
BciT130I CCWGG 1 cut(s) 2798
BciVI GTATCC 2 cut(s) 904, 2394
BclI TGATCA 3 cut(s) 312, 613, 856
BcnI CCSGG 3 cut(s) 372, 1825, 2296
BcoDI GTCTC 8 cut(s) 193, 248, 343, 1341, 1380, 1641, 1789, 2291
BfaI CTAG 5 cut(s) 1205, 1695, 1769, 1994, 2048
BfoI RGCGCY 1 cut(s) 296
BfuAI ACCTGC 1 cut(s) 926
BfuI GTATCC 2 cut(s) 904, 2394
BglI GCCNNNNNGGC 1 cut(s) 1059
BglII AGATCT 1 cut(s) 1904
BlpI GCTNAGC 1 cut(s) 134
Bme1390I CCNGG 4 cut(s) 372, 1825, 2296, 2798
Bme18I GGWCC 5 cut(s) 639, 649, 2238, 2249, 2800
BmgBI CACGTC 1 cut(s) 1512
BmgT120I GGNCC 8 cut(s) 639, 649, 738, 1051, 1297, 2238, 2249, 2800
BmiI GGNNCC 6 cut(s) 408, 473, 651, 1052, 2222, 2251
BmrFI CCNGG 4 cut(s) 372, 1825, 2296, 2798
BmsI GCATC 7 cut(s) 147, 574, 1425, 1580, 1953, 2089, 2732
BpiI GAAGAC 5 cut(s) 83, 576, 2356, 2620, 2937
Bpu1102I GCTNAGC 1 cut(s) 134
Bpu14I TTCGAA 1 cut(s) 2595
BpuEI CTTGAG 3 cut(s) 73, 2413, 2850
BpuMI CCSGG 3 cut(s) 372, 1825, 2296
BsaAI YACGTR 1 cut(s) 379
BsaBI GATNNNNATC 3 cut(s) 894, 1080, 2158
BsaHI GRCGYC 2 cut(s) 108, 273
BsaI GGTCTC 2 cut(s) 248, 2291
BsaJI CCNNGG 3 cut(s) 883, 973, 1898
BsaWI WCCGGW 3 cut(s) 499, 1554, 1978
BsaXI ACNNNNNCTCC 4 cut(s) 898, 924, 928, 954
Bsc4I CCNNNNNNNGG 1 cut(s) 2738
Bse118I RCCGGY 2 cut(s) 474, 2246
Bse3DI GCAATG 2 cut(s) 527, 2241
Bse8I GATNNNNATC 3 cut(s) 894, 1080, 2158
BseAI TCCGGA 2 cut(s) 499, 1978
BseBI CCWGG 1 cut(s) 2798
BseDI CCNNGG 3 cut(s) 883, 973, 1898
BseGI GGATG 8 cut(s) 604, 895, 1081, 1307, 1479, 1595, 2747, 2826
BseJI GATNNNNATC 3 cut(s) 894, 1080, 2158
BseLI CCNNNNNNNGG 1 cut(s) 2738
BseMI GCAATG 2 cut(s) 527, 2241
BseMII CTCAG 5 cut(s) 148, 293, 351, 1624, 1800
BseRI GAGGAG 3 cut(s) 319, 1629, 1632
BseX3I CGGCCG 1 cut(s) 350
BseXI GCAGC 8 cut(s) 39, 511, 978, 981, 1345, 2045, 2515, 2660
BseYI CCCAGC 1 cut(s) 2036
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 5 cut(s) 352, 370, 739, 1053, 1299
BshNI GGYRCC 1 cut(s) 2220
BsiEI CGRYCG 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 2866
BslFI GGGAC 5 cut(s) 145, 387, 1538, 2235, 2278
BslI CCNNNNNNNGG 1 cut(s) 2738
BsmAI GTCTC 8 cut(s) 193, 248, 343, 1341, 1380, 1641, 1789, 2291
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 5 cut(s) 145, 387, 1538, 2235, 2278
BsmI GAATGC 1 cut(s) 870
BsnI GGCC 5 cut(s) 352, 370, 739, 1053, 1299
Bso31I GGTCTC 2 cut(s) 248, 2291
Bsp119I TTCGAA 1 cut(s) 2595
Bsp1286I GDGCHC 1 cut(s) 2866
Bsp13I TCCGGA 2 cut(s) 499, 1978
Bsp1720I GCTNAGC 1 cut(s) 134
Bsp19I CCATGG 2 cut(s) 973, 1898
BspACI CCGC 9 cut(s) 204, 210, 251, 276, 353, 770, 1773, 1970, 2941
BspANI GGCC 5 cut(s) 352, 370, 739, 1053, 1299
BspCNI CTCAG 5 cut(s) 147, 294, 352, 1623, 1799
BspEI TCCGGA 2 cut(s) 499, 1978
BspHI TCATGA 3 cut(s) 559, 1440, 2856
BspLI GGNNCC 6 cut(s) 408, 473, 651, 1052, 2222, 2251
BspMI ACCTGC 1 cut(s) 926
BspPI GGATC 5 cut(s) 189, 401, 414, 1326, 1338
BspT104I TTCGAA 1 cut(s) 2595
BspT107I GGYRCC 1 cut(s) 2220
BspTNI GGTCTC 2 cut(s) 248, 2291
BsrBI CCGCTC 2 cut(s) 251, 2941
BsrDI GCAATG 2 cut(s) 527, 2241
BsrFI RCCGGY 2 cut(s) 474, 2246
BssAI RCCGGY 2 cut(s) 474, 2246
BssECI CCNNGG 3 cut(s) 883, 973, 1898
BssNAI GTATAC 1 cut(s) 2908
BssNI GRCGYC 2 cut(s) 108, 273
BssT1I CCWWGG 2 cut(s) 973, 1898
Bst1107I GTATAC 1 cut(s) 2908
Bst2UI CCWGG 1 cut(s) 2798
Bst4CI ACNGT 9 cut(s) 551, 782, 1447, 1623, 1672, 1731, 1858, 2626, 2837
Bst6I CTCTTC 1 cut(s) 1735
BstACI GRCGYC 2 cut(s) 108, 273
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 1 cut(s) 2595
BstC8I GCNNGC 5 cut(s) 394, 476, 1545, 1936, 1968
BstDEI CTNAG 5 cut(s) 134, 302, 360, 1610, 1786
BstDSI CCRYGG 3 cut(s) 883, 973, 1898
BstF5I GGATG 8 cut(s) 604, 895, 1081, 1307, 1479, 1595, 2747, 2826
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 2 cut(s) 295, 2326
BstMAI GTCTC 8 cut(s) 193, 248, 343, 1341, 1380, 1641, 1789, 2291
BstMCI CGRYCG 1 cut(s) 353
BstNI CCWGG 1 cut(s) 2798
BstNSI RCATGY 3 cut(s) 133, 1938, 2216
BstSCI CCNGG 4 cut(s) 370, 1823, 2294, 2796
BstV1I GCAGC 8 cut(s) 39, 511, 978, 981, 1345, 2045, 2515, 2660
BstV2I GAAGAC 5 cut(s) 83, 576, 2356, 2620, 2937
BstX2I RGATCY 3 cut(s) 181, 406, 1904
BstXI CCANNNNNNTGG 2 cut(s) 890, 980
BstYI RGATCY 3 cut(s) 181, 406, 1904
BstZ17I GTATAC 1 cut(s) 2908
BstZI CGGCCG 1 cut(s) 350
BsuI GTATCC 2 cut(s) 904, 2394
BsuRI GGCC 5 cut(s) 352, 370, 739, 1053, 1299
BtgI CCRYGG 3 cut(s) 883, 973, 1898
BtrI CACGTC 1 cut(s) 1512
BtsCI GGATG 8 cut(s) 604, 895, 1081, 1307, 1479, 1595, 2747, 2826
BtsI GCAGTG 1 cut(s) 1394
BtsIMutI CAGTG 2 cut(s) 1394, 2710
BveI ACCTGC 1 cut(s) 926
Cac8I GCNNGC 5 cut(s) 394, 476, 1545, 1936, 1968
CciI TCATGA 3 cut(s) 559, 1440, 2856
CfoI GCGC 2 cut(s) 295, 2326
Cfr10I RCCGGY 2 cut(s) 474, 2246
Cfr13I GGNCC 8 cut(s) 639, 649, 738, 1051, 1297, 2238, 2249, 2800
CseI GACGC 2 cut(s) 97, 281
Csp6I GTAC 4 cut(s) 834, 999, 2261, 2387
CviQI GTAC 4 cut(s) 834, 999, 2261, 2387
DdeI CTNAG 5 cut(s) 134, 302, 360, 1610, 1786
DraIII CACNNNGTG 1 cut(s) 1845
EaeI YGGCCR 1 cut(s) 350
EagI CGGCCG 1 cut(s) 350
Eam1104I CTCTTC 1 cut(s) 1735
EarI CTCTTC 1 cut(s) 1735
EclXI CGGCCG 1 cut(s) 350
Eco130I CCWWGG 2 cut(s) 973, 1898
Eco31I GGTCTC 2 cut(s) 248, 2291
Eco32I GATATC 2 cut(s) 1093, 2920
Eco47I GGWCC 5 cut(s) 639, 649, 2238, 2249, 2800
Eco52I CGGCCG 1 cut(s) 350
Eco57I CTGAAG 4 cut(s) 95, 971, 2082, 2614
Eco72I CACGTG 1 cut(s) 379
EcoO109I RGGNCCY 2 cut(s) 649, 1051
EcoRI GAATTC 2 cut(s) 449, 2520
EcoRII CCWGG 1 cut(s) 2796
EcoRV GATATC 2 cut(s) 1093, 2920
EcoT14I CCWWGG 2 cut(s) 973, 1898
EcoT22I ATGCAT 4 cut(s) 870, 1026, 1657, 2787
ErhI CCWWGG 2 cut(s) 973, 1898
Esp3I CGTCTC 1 cut(s) 193
FalI AAGNNNNNCTT 2 cut(s) 1566, 1598
FaqI GGGAC 5 cut(s) 145, 387, 1538, 2235, 2278
FauI CCCGC 2 cut(s) 211, 217
FauNDI CATATG 3 cut(s) 841, 1020, 2693
FbaI TGATCA 3 cut(s) 312, 613, 856
FblI GTMKAC 1 cut(s) 2907
FokI GGATG 8 cut(s) 611, 882, 1088, 1294, 1486, 1602, 2754, 2833
FspBI CTAG 5 cut(s) 1205, 1695, 1769, 1994, 2048
GlaI GCGC 2 cut(s) 294, 2325
GsaI CCCAGC 1 cut(s) 2040
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 5 cut(s) 352, 370, 739, 1053, 1299
HgaI GACGC 2 cut(s) 97, 281
HhaI GCGC 2 cut(s) 295, 2326
Hin1I GRCGYC 2 cut(s) 108, 273
Hin6I GCGC 2 cut(s) 293, 2324
HinP1I GCGC 2 cut(s) 293, 2324
HincII GTYRAC 2 cut(s) 778, 1110
HindII GTYRAC 2 cut(s) 778, 1110
HinfI GANTC 7 cut(s) 266, 336, 362, 455, 716, 1912, 2853
HphI GGTGA 4 cut(s) 1544, 1813, 1822, 2449
Hpy166II GTNNAC 9 cut(s) 49, 127, 172, 778, 1110, 1138, 1154, 1683, 2908
Hpy8I GTNNAC 9 cut(s) 49, 127, 172, 778, 1110, 1138, 1154, 1683, 2908
Hpy99I CGWCG 6 cut(s) 101, 104, 107, 113, 236, 275
HpyAV CCTTC 7 cut(s) 655, 909, 1064, 1249, 2057, 2566, 2908
HpyCH4III ACNGT 9 cut(s) 551, 782, 1447, 1623, 1672, 1731, 1858, 2626, 2837
HpyCH4IV ACGT 3 cut(s) 378, 1511, 2303
HpyF3I CTNAG 5 cut(s) 134, 302, 360, 1610, 1786
HpySE526I ACGT 3 cut(s) 378, 1511, 2303
Hsp92I GRCGYC 2 cut(s) 108, 273
HspAI GCGC 2 cut(s) 293, 2324
Kpn2I TCCGGA 2 cut(s) 499, 1978
KroI GCCGGC 1 cut(s) 474
KroNI GCCGGC 1 cut(s) 476
Ksp22I TGATCA 3 cut(s) 312, 613, 856
LmnI GCTCC 8 cut(s) 306, 319, 471, 932, 1540, 1630, 2030, 2938
Lsp1109I GCAGC 8 cut(s) 39, 511, 978, 981, 1345, 2045, 2515, 2660
LweI GCATC 7 cut(s) 147, 574, 1425, 1580, 1953, 2089, 2732
MaeI CTAG 5 cut(s) 1205, 1695, 1769, 1994, 2048
MaeII ACGT 3 cut(s) 378, 1511, 2303
MaeIII GTNAC 5 cut(s) 418, 666, 1731, 2652, 2831
MbiI CCGCTC 2 cut(s) 251, 2941
MflI RGATCY 3 cut(s) 181, 406, 1904
MhlI GDGCHC 1 cut(s) 2866
MlyI GAGTC 3 cut(s) 275, 345, 371
MmeI TCCRAC 4 cut(s) 294, 1451, 2131, 2798
Mph1103I ATGCAT 4 cut(s) 870, 1026, 1657, 2787
MroI TCCGGA 2 cut(s) 499, 1978
MroNI GCCGGC 1 cut(s) 474
MroXI GAANNNNTTC 3 cut(s) 1200, 1380, 1817
MseI TTAA 7 cut(s) 465, 942, 1278, 2460, 2528, 2547, 2962
MslI CAYNNNNRTG 5 cut(s) 888, 978, 1023, 1172, 1664
MspA1I CMGCKG 3 cut(s) 1970, 2036, 2506
MspR9I CCNGG 4 cut(s) 372, 1825, 2296, 2798
Mva1269I GAATGC 1 cut(s) 870
MvaI CCWGG 1 cut(s) 2798
NaeI GCCGGC 1 cut(s) 476
NciI CCSGG 3 cut(s) 372, 1825, 2296
NcoI CCATGG 2 cut(s) 973, 1898
NdeI CATATG 3 cut(s) 841, 1020, 2693
NgoMIV GCCGGC 1 cut(s) 474
NlaIV GGNNCC 6 cut(s) 408, 473, 651, 1052, 2222, 2251
NmeAIII GCCGAG 1 cut(s) 328
NsiI ATGCAT 4 cut(s) 870, 1026, 1657, 2787
NspI RCATGY 3 cut(s) 133, 1938, 2216
NspV TTCGAA 1 cut(s) 2595
PaeI GCATGC 1 cut(s) 1938
PagI TCATGA 3 cut(s) 559, 1440, 2856
PaqCI CACCTGC 1 cut(s) 926
PcsI WCGNNNNNNNCGW 4 cut(s) 105, 108, 228, 2592
PctI GAATGC 1 cut(s) 870
PdiI GCCGGC 1 cut(s) 476
PdmI GAANNNNTTC 3 cut(s) 1200, 1380, 1817
PfeI GAWTC 4 cut(s) 455, 716, 1912, 2853
PflFI GACNNNGTC 1 cut(s) 361
PleI GAGTC 3 cut(s) 274, 344, 370
PmaCI CACGTG 1 cut(s) 379
PmlI CACGTG 1 cut(s) 379
PpsI GAGTC 3 cut(s) 274, 344, 370
Ppu21I YACGTR 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 649
PsiI TTATAA 1 cut(s) 2231
Psp5II RGGWCCY 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 2796
PspCI CACGTG 1 cut(s) 379
PspFI CCCAGC 1 cut(s) 2036
PspGI CCWGG 1 cut(s) 2796
PspN4I GGNNCC 6 cut(s) 408, 473, 651, 1052, 2222, 2251
PspPI GGNCC 8 cut(s) 639, 649, 738, 1051, 1297, 2238, 2249, 2800
PspPPI RGGWCCY 1 cut(s) 649
PsuI RGATCY 3 cut(s) 181, 406, 1904
PsyI GACNNNGTC 1 cut(s) 361
PvuII CAGCTG 2 cut(s) 2036, 2506
RsaI GTAC 4 cut(s) 835, 1000, 2262, 2388
RsaNI GTAC 4 cut(s) 834, 999, 2261, 2387
RseI CAYNNNNRTG 5 cut(s) 888, 978, 1023, 1172, 1664
SaqAI TTAA 7 cut(s) 465, 942, 1278, 2460, 2528, 2547, 2962
Sau96I GGNCC 8 cut(s) 639, 649, 738, 1051, 1297, 2238, 2249, 2800
SchI GAGTC 3 cut(s) 275, 345, 371
ScrFI CCNGG 4 cut(s) 372, 1825, 2296, 2798
SduI GDGCHC 1 cut(s) 2866
SfaNI GCATC 7 cut(s) 147, 574, 1425, 1580, 1953, 2089, 2732
SfuI TTCGAA 1 cut(s) 2595
SinI GGWCC 5 cut(s) 639, 649, 2238, 2249, 2800
SmiMI CAYNNNNRTG 5 cut(s) 888, 978, 1023, 1172, 1664
SmlI CTYRAG 3 cut(s) 52, 2428, 2865
SmoI CTYRAG 3 cut(s) 52, 2428, 2865
SphI GCATGC 1 cut(s) 1938
SsiI CCGC 9 cut(s) 204, 210, 251, 276, 353, 770, 1773, 1970, 2941
SspMI CTAG 5 cut(s) 1205, 1695, 1769, 1994, 2048
StyD4I CCNGG 4 cut(s) 370, 1823, 2294, 2796
StyI CCWWGG 2 cut(s) 973, 1898
TaaI ACNGT 9 cut(s) 551, 782, 1447, 1623, 1672, 1731, 1858, 2626, 2837
TaiI ACGT 3 cut(s) 381, 1514, 2306
TaqI TCGA 6 cut(s) 144, 264, 365, 453, 1384, 2595
TatI WGTACW 1 cut(s) 2386
TauI GCSGC 3 cut(s) 278, 355, 1775
TfiI GAWTC 4 cut(s) 455, 716, 1912, 2853
Tru1I TTAA 7 cut(s) 465, 942, 1278, 2460, 2528, 2547, 2962
Tru9I TTAA 7 cut(s) 465, 942, 1278, 2460, 2528, 2547, 2962
TscAI CASTG 2 cut(s) 1401, 2710
TseI GCWGC 8 cut(s) 27, 524, 966, 969, 1358, 2033, 2503, 2648
TspGWI ACGGA 4 cut(s) 900, 923, 1752, 2210
TspRI CASTG 2 cut(s) 1401, 2710
Tth111I GACNNNGTC 1 cut(s) 361
VpaK11BI GGWCC 5 cut(s) 639, 649, 2238, 2249, 2800
XapI RAATTY 5 cut(s) 449, 1974, 2072, 2520, 2572
XbaI TCTAGA 1 cut(s) 2047
XceI RCATGY 3 cut(s) 133, 1938, 2216
XmiI GTMKAC 1 cut(s) 2907
XmnI GAANNNNTTC 3 cut(s) 1200, 1380, 1817
XspI CTAG 5 cut(s) 1205, 1695, 1769, 1994, 2048
Zsp2I ATGCAT 4 cut(s) 870, 1026, 1657, 2787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.