RchiOBHm_Chr7g0219571

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002335
Physical Location & Seq
Reverse (-)
38122893 .. 38125199
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19651

Sequence Viewer

Length: 1245 bp
ATGGTCATGCACATCGCCCCCTCTTCCTCCATCTATCTCAATAATGTTGCGGTTGTTGACAGTATAGCTGAAAGGGTTTATAATCTTCTTGAAGATTACTACAAGGACAATCGTACAGCATACGTATTTACTGCTGATCATGGAATGCATGATAAAGGTAGCCACGGAGATGGACATCCTTCAAACACCGATACTCCCCTTGTTGTTTGGGGAGCAGGTGTTAAACACCCAAAGCCTATTTCCAGCAGCAGCCATGGAGATGGACATTCTGATTGTGGTACTCGTTTTGTTGATGACCATATGCATGACACGCCAACGCCTAAAGAATGGGGCCTTCATGGCATTGAAAGGGTGGATGTCAATCAAACTGATATCGCACCTCTCATGTCAACACTTCTTGGATTGCCATGTCCTGTAAACTCGGTCGGGAGTTTACCCCTTGATTACATAGACATGAAAAAGACAGATGAAGTTGAAGCTGTTCTAGCCAATACAAAGCAAGTTCTCAATCAATTTGTTCGGAAATCACAAACCAAGGAGGCAACTTCATTATATTTTAAGCCTTTCAAACCATTGGGCCATTATTCAACCCTCTTGGATCAGATTGAGGATCATATATCTAATGGAGACTATGAAGCTGCAAGGAAACTATCTGAAAATCTCCGAGACTTGGCACTGCAAGGACTTCGTTATTTTCAAACTTATGATTGGCTGATGCTCATGACTGTAATTATTCTTGGGTATATTGGTTGGATGACCTATATTGTTCTCCATGTGTTGCAATCTTACACGTCATTGGCAGGGGATATATTGACAAAGGAGCAAGCAGATCACTTGACAGATTATACCAGAAAAGTACAACTTTGTGGATGCTTGTTTCTGGGACTACTTAGGGTCATACTGTTCATGGAGCAGGCTCCTCCTCTTTACCATGCATACACAACAATGACAGTATTTTTGTGGACACAAATATCTAGTGAATATCGGTTCATAAAAGCATTATGGAAACAGTTACATGGAAGAGAAATAAATTATTTCACAAAAGTTGGAGCCGCCTGTGCTGTCTCAGTATTCATACTTGAATATCTGGTGAATAGCTTCACCGAGAGGAAGCTCTACACATGGTGTTTTCTGACTGTGGGGGTCATAGCCTTTCTTTATCTTTTCAAGTCAATTCCATGGAGATCTGGGATTCCATTCTTTGTTTGTGGTGCATGCTGGTTCTTATCAGTATCTGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest PigN Sulfatase

Protein Analysis

414

Amino Acids

46.96

Weight (kDa)

6.08

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfatase PF00884 12 - 82 3.8e-07 Sulfatase
PigN PF04987 227 - 411 5e-34 Phosphatidylinositolglycan class N (PIG-N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
AarI CACCTGC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 206
AciI CCGC 2 cut(s) 50, 1053
AclWI GGATC 2 cut(s) 606, 618
AdeI CACNNNGTG 1 cut(s) 1125
AfaI GTAC 3 cut(s) 115, 280, 858
AfiI CCNNNNNNNGG 1 cut(s) 670
AflIII ACRYGT 1 cut(s) 789
AgsI TTSAA 9 cut(s) 92, 183, 347, 476, 568, 588, 698, 1082, 1168
AjiI CACGTC 1 cut(s) 792
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AluBI AGCT 5 cut(s) 68, 479, 638, 1098, 1114
AluI AGCT 5 cut(s) 68, 479, 638, 1098, 1114
Alw26I GTCTC 3 cut(s) 621, 660, 1069
AlwI GGATC 2 cut(s) 606, 618
AlwNI CAGNNNCTG 1 cut(s) 1235
AoxI GGCC 2 cut(s) 331, 577
ApeKI GCWGC 3 cut(s) 246, 249, 638
ArsI GACNNNNNNTTYG 2 cut(s) 940, 972
Asp700I GAANNNNTTC 2 cut(s) 480, 1097
AspS9I GGNCC 2 cut(s) 331, 577
AsuHPI GGTGA 2 cut(s) 1093, 1102
BbvI GCAGC 3 cut(s) 258, 261, 625
BccI CCATC 3 cut(s) 38, 164, 254
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 3 cut(s) 621, 660, 1069
BfaI CTAG 2 cut(s) 485, 975
BfuAI ACCTGC 1 cut(s) 206
BglI GCCNNNNNGGC 1 cut(s) 339
BglII AGATCT 1 cut(s) 1184
BisI GCNGC 4 cut(s) 247, 250, 639, 1053
BlsI GCNGC 4 cut(s) 248, 251, 640, 1054
BmgBI CACGTC 1 cut(s) 792
BmgT120I GGNCC 2 cut(s) 331, 577
BmiI GGNNCC 3 cut(s) 332, 918, 1051
BmsI GCATC 2 cut(s) 705, 860
BsaAI YACGTR 1 cut(s) 124
BsaBI GATNNNNATC 2 cut(s) 174, 360
BsaJI CCNNGG 4 cut(s) 163, 253, 534, 1178
BsaXI ACNNNNNCTCC 4 cut(s) 178, 204, 208, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 670
Bse8I GATNNNNATC 2 cut(s) 174, 360
BseDI CCNNGG 4 cut(s) 163, 253, 534, 1178
BseGI GGATG 4 cut(s) 175, 361, 759, 875
BseJI GATNNNNATC 2 cut(s) 174, 360
BseLI CCNNNNNNNGG 1 cut(s) 670
BseMII CTCAG 1 cut(s) 1080
BseRI GAGGAG 2 cut(s) 909, 912
BseXI GCAGC 3 cut(s) 258, 261, 625
Bsh1285I CGRYCG 1 cut(s) 426
BshFI GGCC 2 cut(s) 333, 579
BsiEI CGRYCG 1 cut(s) 426
BslFI GGGAC 1 cut(s) 897
BslI CCNNNNNNNGG 1 cut(s) 670
BsmAI GTCTC 3 cut(s) 621, 660, 1069
BsmFI GGGAC 1 cut(s) 897
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 2 cut(s) 333, 579
Bsp143I GATC 5 cut(s) 136, 598, 610, 829, 1184
Bsp19I CCATGG 2 cut(s) 253, 1178
BspACI CCGC 2 cut(s) 50, 1053
BspANI GGCC 2 cut(s) 333, 579
BspCNI CTCAG 1 cut(s) 1079
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 3 cut(s) 332, 918, 1051
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 606, 618
BssECI CCNNGG 4 cut(s) 163, 253, 534, 1178
BssMI GATC 5 cut(s) 136, 598, 610, 829, 1184
BssT1I CCWWGG 3 cut(s) 253, 534, 1178
Bst4CI ACNGT 6 cut(s) 62, 727, 903, 952, 1011, 1138
Bst6I CTCTTC 2 cut(s) 28, 1015
BstBAI YACGTR 1 cut(s) 124
BstC8I GCNNGC 3 cut(s) 825, 915, 1216
BstDEI CTNAG 2 cut(s) 890, 1066
BstDSI CCRYGG 3 cut(s) 163, 253, 1178
BstF5I GGATG 4 cut(s) 175, 361, 759, 875
BstKTI GATC 5 cut(s) 139, 601, 613, 832, 1187
BstMAI GTCTC 3 cut(s) 621, 660, 1069
BstMBI GATC 5 cut(s) 136, 598, 610, 829, 1184
BstMCI CGRYCG 1 cut(s) 426
BstMWI GCNNNNNNNGC 4 cut(s) 310, 339, 485, 1058
BstNSI RCATGY 1 cut(s) 1218
BstSNI TACGTA 1 cut(s) 124
BstV1I GCAGC 3 cut(s) 258, 261, 625
BstX2I RGATCY 1 cut(s) 1184
BstXI CCANNNNNNTGG 2 cut(s) 170, 260
BstYI RGATCY 1 cut(s) 1184
BsuRI GGCC 2 cut(s) 333, 579
BtgI CCRYGG 3 cut(s) 163, 253, 1178
BtrI CACGTC 1 cut(s) 792
BtsCI GGATG 4 cut(s) 175, 361, 759, 875
BtsI GCAGTG 1 cut(s) 674
BtsIMutI CAGTG 1 cut(s) 674
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 3 cut(s) 825, 915, 1216
CaiI CAGNNNCTG 1 cut(s) 1235
CciI TCATGA 1 cut(s) 720
Cfr13I GGNCC 2 cut(s) 331, 577
Csp6I GTAC 3 cut(s) 114, 279, 857
CviQI GTAC 3 cut(s) 114, 279, 857
DdeI CTNAG 2 cut(s) 890, 1066
DpnI GATC 5 cut(s) 138, 600, 612, 831, 1186
DpnII GATC 5 cut(s) 136, 598, 610, 829, 1184
DraIII CACNNNGTG 1 cut(s) 1125
Eam1104I CTCTTC 2 cut(s) 28, 1015
EarI CTCTTC 2 cut(s) 28, 1015
Eco105I TACGTA 1 cut(s) 124
Eco130I CCWWGG 3 cut(s) 253, 534, 1178
Eco32I GATATC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 331
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 3 cut(s) 253, 534, 1178
EcoT22I ATGCAT 3 cut(s) 150, 306, 937
ErhI CCWWGG 3 cut(s) 253, 534, 1178
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FaqI GGGAC 1 cut(s) 897
FauNDI CATATG 1 cut(s) 300
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 4 cut(s) 247, 250, 639, 1053
FokI GGATG 4 cut(s) 162, 368, 766, 882
Fsp4HI GCNGC 4 cut(s) 247, 250, 639, 1053
FspBI CTAG 2 cut(s) 485, 975
GluI GCNGC 4 cut(s) 247, 250, 639, 1053
HaeIII GGCC 2 cut(s) 333, 579
HincII GTYRAC 2 cut(s) 58, 390
HindII GTYRAC 2 cut(s) 58, 390
HinfI GANTC 1 cut(s) 1192
HphI GGTGA 2 cut(s) 1093, 1102
Hpy166II GTNNAC 5 cut(s) 58, 390, 418, 434, 963
Hpy188I TCNGA 7 cut(s) 271, 522, 603, 655, 665, 1134, 1237
Hpy188III TCNNGA 3 cut(s) 89, 427, 721
Hpy8I GTNNAC 5 cut(s) 58, 390, 418, 434, 963
HpyAV CCTTC 2 cut(s) 189, 344
HpyCH4III ACNGT 6 cut(s) 62, 727, 903, 952, 1011, 1138
HpyCH4IV ACGT 2 cut(s) 123, 791
HpyCH4V TGCA 8 cut(s) 10, 148, 304, 641, 679, 781, 935, 1214
HpyF10VI GCNNNNNNNGC 4 cut(s) 310, 339, 485, 1058
HpyF3I CTNAG 2 cut(s) 890, 1066
HpySE526I ACGT 2 cut(s) 123, 791
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 5 cut(s) 136, 598, 610, 829, 1184
LmnI GCTCC 5 cut(s) 212, 820, 910, 922, 1049
Lsp1109I GCAGC 3 cut(s) 258, 261, 625
LweI GCATC 2 cut(s) 705, 860
MaeI CTAG 2 cut(s) 485, 975
MaeII ACGT 2 cut(s) 123, 791
MaeIII GTNAC 1 cut(s) 1011
MalI GATC 5 cut(s) 138, 600, 612, 831, 1186
MboI GATC 5 cut(s) 136, 598, 610, 829, 1184
MboII GAAGA 4 cut(s) 15, 77, 104, 1032
MflI RGATCY 1 cut(s) 1184
MluCI AATT 4 cut(s) 512, 729, 1030, 1173
MmeI TCCRAC 2 cut(s) 731, 1027
MnlI CCTC 9 cut(s) 31, 37, 390, 532, 601, 602, 930, 933, 1101
Mph1103I ATGCAT 3 cut(s) 150, 306, 937
MroXI GAANNNNTTC 2 cut(s) 480, 1097
MseI TTAA 2 cut(s) 222, 558
MslI CAYNNNNRTG 5 cut(s) 168, 258, 303, 452, 944
Mva1269I GAATGC 1 cut(s) 150
MwoI GCNNNNNNNGC 4 cut(s) 310, 339, 485, 1058
NcoI CCATGG 2 cut(s) 253, 1178
NdeI CATATG 1 cut(s) 300
NdeII GATC 5 cut(s) 136, 598, 610, 829, 1184
NlaIV GGNNCC 3 cut(s) 332, 918, 1051
NsiI ATGCAT 3 cut(s) 150, 306, 937
NspI RCATGY 1 cut(s) 1218
PaeI GCATGC 1 cut(s) 1218
PagI TCATGA 1 cut(s) 720
PaqCI CACCTGC 1 cut(s) 206
PctI GAATGC 1 cut(s) 150
PdmI GAANNNNTTC 2 cut(s) 480, 1097
PfeI GAWTC 1 cut(s) 1192
PkrI GCNGC 4 cut(s) 248, 251, 640, 1054
Ppu21I YACGTR 1 cut(s) 124
PsiI TTATAA 1 cut(s) 81
PspN4I GGNNCC 3 cut(s) 332, 918, 1051
PspPI GGNCC 2 cut(s) 331, 577
PstNI CAGNNNCTG 1 cut(s) 1235
PsuI RGATCY 1 cut(s) 1184
RsaI GTAC 3 cut(s) 115, 280, 858
RsaNI GTAC 3 cut(s) 114, 279, 857
RseI CAYNNNNRTG 5 cut(s) 168, 258, 303, 452, 944
SaqAI TTAA 2 cut(s) 222, 558
SatI GCNGC 4 cut(s) 247, 250, 639, 1053
Sau3AI GATC 5 cut(s) 136, 598, 610, 829, 1184
Sau96I GGNCC 2 cut(s) 331, 577
SfaNI GCATC 2 cut(s) 705, 860
SmiMI CAYNNNNRTG 5 cut(s) 168, 258, 303, 452, 944
SnaBI TACGTA 1 cut(s) 124
SphI GCATGC 1 cut(s) 1218
Sse9I AATT 4 cut(s) 512, 729, 1030, 1173
SsiI CCGC 2 cut(s) 50, 1053
SspMI CTAG 2 cut(s) 485, 975
StyI CCWWGG 3 cut(s) 253, 534, 1178
TaaI ACNGT 6 cut(s) 62, 727, 903, 952, 1011, 1138
TaiI ACGT 2 cut(s) 126, 794
TaqII GACCGA 1 cut(s) 412
TasI AATT 4 cut(s) 512, 729, 1030, 1173
TatI WGTACW 1 cut(s) 856
TauI GCSGC 1 cut(s) 1055
TfiI GAWTC 1 cut(s) 1192
Tru1I TTAA 2 cut(s) 222, 558
Tru9I TTAA 2 cut(s) 222, 558
TscAI CASTG 1 cut(s) 681
TseI GCWGC 3 cut(s) 246, 249, 638
TspDTI ATGAA 8 cut(s) 326, 470, 483, 537, 648, 895, 979, 1063
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 681
XceI RCATGY 1 cut(s) 1218
XmnI GAANNNNTTC 2 cut(s) 480, 1097
XspI CTAG 2 cut(s) 485, 975
Zsp2I ATGCAT 3 cut(s) 150, 306, 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.