MD09G1071100.v1.1

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003991
Physical Location & Seq
Forward (+)
4915968 .. 4917731
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1071100.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGACGAGGTGATGACGGCTGCGGATGCGACGACGTCGTCTTCGCCGTCGACGGCGGCGGCGATCCCATCGAACCTCGCTCTCCTCGCTGCGCTCCTAGCATGCGCTCTCGCTCAGTTCCTGAAGCTCTTCACCACCTGGTACAAAGAAAAGAGATGGGACTCGAAGAAAATGCTCGATTCCGGCGGGATGCCGTCGTCACATTCCGCCACGGTGACGGCTCTGGCCGTCGCCGTTGGCCTTGAGGACGGCACCGGAGGACCTGCCTTCGCCATTGCCTTTGTCTTAGCGTGCATTGTTATGTATGATGCCTCAGGAGTGAGACTTCATGCTGGTCGGCAAGCCGAGTTGCTGAACCTAATTGTGTGTGAGCTTCCTCCAGAGCATCCTTTGTCCAGCGTTAGACCTCTGCGCGATTCACTTGGACATACTCCGCTACAGGTTGCTGCAGGTGCTGTGTTGGGATGCATAGTAGCTTACCTAATGAGTAACACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

167

Amino Acids

17.27

Weight (kDa)

6.03

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 25 - 158 2.2e-49 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 440
AasI GACNNNNNNGTC 1 cut(s) 37
AatII GACGTC 1 cut(s) 38
Acc36I ACCTGC 2 cut(s) 271, 440
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 50
AccII CGCG 1 cut(s) 414
AciI CCGC 6 cut(s) 23, 56, 59, 186, 207, 434
AclWI GGATC 1 cut(s) 58
AcoI YGGCCR 1 cut(s) 225
AcuI CTGAAG 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 1 cut(s) 143
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 3 cut(s) 127, 373, 476
AluI AGCT 3 cut(s) 127, 373, 476
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 1 cut(s) 58
AlwNI CAGNNNCTG 2 cut(s) 121, 455
AoxI GGCC 2 cut(s) 225, 238
ApeKI GCWGC 3 cut(s) 20, 89, 446
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 3 cut(s) 94, 107, 414
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 3 cut(s) 22, 124, 226
AvaII GGWCC 1 cut(s) 260
AxyI CCTNAGG 1 cut(s) 313
BanI GGYRCC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 7, 76, 433
BccI CCATC 2 cut(s) 76, 150
BceAI ACGGC 8 cut(s) 31, 33, 69, 178, 212, 218, 234, 265
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BciT130I CCWGG 1 cut(s) 139
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 2 cut(s) 437, 447
BfuAI ACCTGC 2 cut(s) 271, 440
BisI GCNGC 5 cut(s) 21, 57, 60, 90, 447
BlsI GCNGC 5 cut(s) 22, 58, 61, 91, 448
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 5 cut(s) 16, 180, 298, 394, 455
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 1 cut(s) 254
Bse21I CCTNAGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 4 cut(s) 31, 195, 385, 470
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 2 cut(s) 128, 327
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 3 cut(s) 7, 76, 433
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 2 cut(s) 227, 240
BshNI GGYRCC 1 cut(s) 251
BsiSI CCGG 2 cut(s) 183, 255
BslFI GGGAC 1 cut(s) 173
BsmAI GTCTC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 2 cut(s) 227, 240
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 6 cut(s) 23, 56, 59, 186, 207, 434
BspANI GGCC 2 cut(s) 227, 240
BspCNI CTCAG 2 cut(s) 127, 326
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 253
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 2 cut(s) 271, 440
BspPI GGATC 1 cut(s) 58
BspQI GCTCTTC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 273
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 63
BssNI GRCGYC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 3 cut(s) 103, 292, 342
BstDEI CTNAG 3 cut(s) 114, 286, 313
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 4 cut(s) 31, 195, 385, 470
BstFNI CGCG 1 cut(s) 414
BstHHI GCGC 3 cut(s) 94, 107, 414
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 4 cut(s) 26, 86, 98, 452
BstNI CCWGG 1 cut(s) 139
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 137
BstSFI CTRYAG 2 cut(s) 437, 447
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 3 cut(s) 7, 76, 433
BstV2I GAAGAC 1 cut(s) 33
Bsu36I CCTNAGG 1 cut(s) 313
BsuRI GGCC 2 cut(s) 227, 240
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 4 cut(s) 31, 195, 385, 470
BveI ACCTGC 2 cut(s) 271, 440
Cac8I GCNNGC 3 cut(s) 103, 292, 342
CaiI CAGNNNCTG 2 cut(s) 121, 455
CfoI GCGC 3 cut(s) 94, 107, 414
Cfr13I GGNCC 1 cut(s) 260
CsiI ACCWGGT 1 cut(s) 137
Csp6I GTAC 1 cut(s) 142
CviAII CATG 2 cut(s) 102, 329
CviJI RGCY 8 cut(s) 20, 127, 221, 227, 240, 344, 373, 476
CviKI_1 RGCY 8 cut(s) 20, 127, 221, 227, 240, 344, 373, 476
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 3 cut(s) 114, 286, 313
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
DrdI GACNNNNNNGTC 1 cut(s) 37
DseDI GACNNNNNNGTC 1 cut(s) 37
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
EciI GGCGGA 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 143
Eco81I CCTNAGG 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 470
FaeI CATG 2 cut(s) 105, 332
FaiI YATR 6 cut(s) 103, 302, 306, 330, 429, 470
FaqI GGGAC 1 cut(s) 173
FatI CATG 2 cut(s) 101, 328
FauI CCCGC 1 cut(s) 179
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 5 cut(s) 21, 57, 60, 90, 447
FokI GGATG 4 cut(s) 38, 202, 372, 477
Fsp4HI GCNGC 5 cut(s) 21, 57, 60, 90, 447
FspBI CTAG 1 cut(s) 98
GlaI GCGC 3 cut(s) 93, 106, 413
GluI GCNGC 5 cut(s) 21, 57, 60, 90, 447
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 2 cut(s) 227, 240
HapII CCGG 2 cut(s) 183, 255
HhaI GCGC 3 cut(s) 94, 107, 414
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 2 cut(s) 105, 332
Hin6I GCGC 3 cut(s) 92, 105, 412
HinP1I GCGC 3 cut(s) 92, 105, 412
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 3 cut(s) 161, 179, 416
HpaII CCGG 2 cut(s) 183, 255
HphI GGTGA 3 cut(s) 22, 124, 226
Hpy166II GTNNAC 1 cut(s) 51
Hpy188III TCNNGA 3 cut(s) 121, 315, 380
Hpy8I GTNNAC 1 cut(s) 51
Hpy99I CGWCG 7 cut(s) 34, 37, 40, 52, 55, 199, 233
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 3 cut(s) 294, 449, 468
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 86, 98, 452
HpyF3I CTNAG 3 cut(s) 114, 286, 313
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 2 cut(s) 105, 332
HspAI GCGC 3 cut(s) 92, 105, 412
Kzo9I GATC 1 cut(s) 63
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 3 cut(s) 7, 76, 433
LweI GCATC 5 cut(s) 16, 180, 298, 394, 455
MabI ACCWGGT 1 cut(s) 137
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 3 cut(s) 198, 214, 488
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 3 cut(s) 33, 121, 178
MluCI AATT 1 cut(s) 360
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 7 cut(s) 86, 95, 238, 251, 322, 387, 417
Mph1103I ATGCAT 1 cut(s) 470
MroXI GAANNNNTTC 1 cut(s) 128
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 2 cut(s) 183, 255
MspR9I CCNGG 1 cut(s) 139
MvaI CCWGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 4 cut(s) 26, 86, 98, 452
NdeII GATC 1 cut(s) 63
NlaIII CATG 2 cut(s) 105, 332
NlaIV GGNNCC 1 cut(s) 253
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 2 cut(s) 198, 214
NsiI ATGCAT 1 cut(s) 470
NspI RCATGY 1 cut(s) 105
PaeI GCATGC 1 cut(s) 105
PaqCI CACCTGC 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 59
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 179, 416
PflFI GACNNNGTC 2 cut(s) 34, 37
PkrI GCNGC 5 cut(s) 22, 58, 61, 91, 448
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 1 cut(s) 260
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 2 cut(s) 121, 455
PsyI GACNNNGTC 2 cut(s) 34, 37
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 299
SalI GTCGAC 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 134
SatI GCNGC 5 cut(s) 21, 57, 60, 90, 447
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 139
SexAI ACCWGGT 1 cut(s) 137
SfaNI GCATC 5 cut(s) 16, 180, 298, 394, 455
SfcI CTRYAG 2 cut(s) 437, 447
SgrDI CGTCGACG 1 cut(s) 49
SinI GGWCC 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SphI GCATGC 1 cut(s) 105
Sse9I AATT 1 cut(s) 360
SsiI CCGC 6 cut(s) 23, 56, 59, 186, 207, 434
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 137
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 38
TaqI TCGA 4 cut(s) 50, 71, 164, 177
TasI AATT 1 cut(s) 360
TauI GCSGC 2 cut(s) 59, 62
TfiI GAWTC 2 cut(s) 179, 416
TseFI GTSAC 2 cut(s) 198, 214
TseI GCWGC 3 cut(s) 20, 89, 446
Tsp45I GTSAC 2 cut(s) 198, 214
TspDTI ATGAA 1 cut(s) 317
Tth111I GACNNNGTC 2 cut(s) 34, 37
VpaK11BI GGWCC 1 cut(s) 260
XceI RCATGY 1 cut(s) 105
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 1 cut(s) 98
ZraI GACGTC 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.