Rh5DG288300

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003991
Physical Location & Seq
Reverse (-)
35867443 .. 35870100
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG288300.1

Sequence Viewer

Length: 549 bp
ATGGACGAGGTGATCACAGCTGCTGATGCTTCCATCAATGGATCCAAGTCCTCCATCATTCCATACAACCTCCCTCTCCTCTCCGCCTTCCTCTCTTGCGCTCTCGCCCAGTTCCTCAAGCTTTTCACCACCTGGTACAAAGAGAAAAGATGGGACTCTAGAAGGATGCTTAGCTCTGGAGGAATGCCGTCGTCGCATTCGTCCACTGTGACGGCACTTGCAGTTGCTATTGGTTTCCAGGACGGAACCGGAGGGTCGACATTTGCCATTGCAGTGGTCTTGGCATGTGTTGTCATGTATGATGCTACTGGTGTAAGACTCCATGCTGGTCGTCAAGCTGAATTATTAAATCAGATAGTGTGTGAGCTACCTCCAGAACACCCGGTTTCTAGTGTTAGACCTCTACGGGATTCACTTGGTCATACTCCATTGCAGGTTTGCTTCAATTTTCATGTTCATGATCATTCACACTTGATTTTAATGGAAACGACTAATGGTCAGAAACTTGGCCGTGAAGATTATAACTTCTATGTCGGATGTGCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

182

Amino Acids

19.81

Weight (kDa)

6.42

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 23 - 148 9.1e-46 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
Acc36I ACCTGC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 257
AciI CCGC 1 cut(s) 84
AclWI GGATC 2 cut(s) 36, 49
AcoI YGGCCR 1 cut(s) 508
AfaI GTAC 1 cut(s) 137
AgsI TTSAA 1 cut(s) 445
AhdI GACNNNNNGTC 1 cut(s) 495
AjnI CCWGG 2 cut(s) 131, 237
AluBI AGCT 5 cut(s) 20, 121, 174, 338, 367
AluI AGCT 5 cut(s) 20, 121, 174, 338, 367
AlwI GGATC 2 cut(s) 36, 49
AlwNI CAGNNNCTG 1 cut(s) 23
AoxI GGCC 1 cut(s) 508
ApeKI GCWGC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 2 cut(s) 22, 118
BamHI GGATCC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 7
BccI CCATC 3 cut(s) 41, 62, 144
BceAI ACGGC 3 cut(s) 172, 228, 495
BciT130I CCWGG 2 cut(s) 133, 239
BclI TGATCA 2 cut(s) 12, 460
BcnI CCSGG 1 cut(s) 383
BfaI CTAG 3 cut(s) 159, 390, 547
BfuAI ACCTGC 1 cut(s) 424
BisI GCNGC 1 cut(s) 21
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 3 cut(s) 133, 239, 383
BmeRI GACNNNNNGTC 1 cut(s) 495
BmiI GGNNCC 2 cut(s) 43, 247
BmrFI CCNGG 3 cut(s) 133, 239, 383
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 3 cut(s) 16, 156, 292
BmuI ACTGGG 1 cut(s) 103
BpmI CTGGAG 2 cut(s) 198, 357
Bpu1102I GCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 383
BsaWI WCCGGW 1 cut(s) 248
Bse1I ACTGG 2 cut(s) 109, 313
Bse3DI GCAATG 2 cut(s) 267, 428
BseBI CCWGG 2 cut(s) 133, 239
BseGI GGATG 2 cut(s) 171, 542
BseMI GCAATG 2 cut(s) 267, 428
BseNI ACTGG 2 cut(s) 109, 313
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 510
BsiSI CCGG 2 cut(s) 249, 383
BslFI GGGAC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 189, 196
BsnI GGCC 1 cut(s) 510
Bsp143I GATC 3 cut(s) 12, 41, 460
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 1 cut(s) 84
BspANI GGCC 1 cut(s) 510
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 43, 247
BspMI ACCTGC 1 cut(s) 424
BspPI GGATC 2 cut(s) 36, 49
BsrDI GCAATG 2 cut(s) 267, 428
BsrI ACTGG 2 cut(s) 109, 313
BssMI GATC 3 cut(s) 12, 41, 460
Bst2UI CCWGG 2 cut(s) 133, 239
Bst4CI ACNGT 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 170
BstF5I GGATG 2 cut(s) 171, 542
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 3 cut(s) 15, 44, 463
BstMBI GATC 3 cut(s) 12, 41, 460
BstMWI GCNNNNNNNGC 2 cut(s) 26, 193
BstNI CCWGG 2 cut(s) 133, 239
BstNSI RCATGY 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 131, 237, 381
BstV1I GCAGC 1 cut(s) 7
BstX2I RGATCY 1 cut(s) 41
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 1 cut(s) 41
BsuRI GGCC 1 cut(s) 510
BtsCI GGATG 2 cut(s) 171, 542
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 204, 279
BveI ACCTGC 1 cut(s) 424
CaiI CAGNNNCTG 1 cut(s) 23
CciI TCATGA 1 cut(s) 457
CfoI GCGC 1 cut(s) 101
CsiI ACCWGGT 1 cut(s) 131
Csp6I GTAC 1 cut(s) 136
CviAII CATG 5 cut(s) 285, 295, 323, 452, 458
CviJI RGCY 6 cut(s) 20, 121, 174, 338, 367, 510
CviKI_1 RGCY 6 cut(s) 20, 121, 174, 338, 367, 510
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 170
DpnI GATC 3 cut(s) 14, 43, 462
DpnII GATC 3 cut(s) 12, 41, 460
DriI GACNNNNNGTC 1 cut(s) 495
EaeI YGGCCR 1 cut(s) 508
Eam1105I GACNNNNNGTC 1 cut(s) 495
EciI GGCGGA 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 131, 237
FaeI CATG 5 cut(s) 288, 298, 326, 455, 461
FaqI GGGAC 1 cut(s) 167
FatI CATG 5 cut(s) 284, 294, 322, 451, 457
FbaI TGATCA 2 cut(s) 12, 460
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 178
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 3 cut(s) 159, 390, 547
GlaI GCGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 21
GsuI CTGGAG 2 cut(s) 198, 357
HaeIII GGCC 1 cut(s) 510
HapII CCGG 2 cut(s) 249, 383
HhaI GCGC 1 cut(s) 101
Hin1II CATG 5 cut(s) 288, 298, 326, 455, 461
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 3 cut(s) 155, 318, 410
HpaII CCGG 2 cut(s) 249, 383
HphI GGTGA 2 cut(s) 22, 118
Hpy166II GTNNAC 2 cut(s) 204, 258
Hpy188I TCNGA 3 cut(s) 354, 501, 536
Hpy188III TCNNGA 4 cut(s) 159, 177, 374, 458
Hpy8I GTNNAC 2 cut(s) 204, 258
Hpy99I CGWCG 2 cut(s) 193, 196
HpyAV CCTTC 2 cut(s) 97, 156
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4V TGCA 3 cut(s) 221, 272, 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 193
HpyF3I CTNAG 1 cut(s) 170
Hsp92II CATG 5 cut(s) 288, 298, 326, 455, 461
HspAI GCGC 1 cut(s) 99
Ksp22I TGATCA 2 cut(s) 12, 460
Kzo9I GATC 3 cut(s) 12, 41, 460
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 3 cut(s) 16, 156, 292
MabI ACCWGGT 1 cut(s) 131
MaeI CTAG 3 cut(s) 159, 390, 547
MaeIII GTNAC 1 cut(s) 208
MalI GATC 3 cut(s) 14, 43, 462
MboI GATC 3 cut(s) 12, 41, 460
MboII GAAGA 1 cut(s) 527
MflI RGATCY 1 cut(s) 41
MluCI AATT 2 cut(s) 341, 445
MlyI GAGTC 2 cut(s) 149, 312
MmeI TCCRAC 1 cut(s) 514
MseI TTAA 2 cut(s) 347, 479
MslI CAYNNNNRTG 2 cut(s) 272, 456
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 2 cut(s) 249, 383
MspR9I CCNGG 3 cut(s) 133, 239, 383
Mva1269I GAATGC 2 cut(s) 189, 196
MvaI CCWGG 2 cut(s) 133, 239
MwoI GCNNNNNNNGC 2 cut(s) 26, 193
NciI CCSGG 1 cut(s) 383
NdeII GATC 3 cut(s) 12, 41, 460
NlaIII CATG 5 cut(s) 288, 298, 326, 455, 461
NlaIV GGNNCC 2 cut(s) 43, 247
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 288
PagI TCATGA 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PctI GAATGC 2 cut(s) 189, 196
PfeI GAWTC 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 237
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 2 cut(s) 149, 312
PpsI GAGTC 2 cut(s) 149, 312
PsiI TTATAA 1 cut(s) 522
Psp6I CCWGG 2 cut(s) 131, 237
PspGI CCWGG 2 cut(s) 131, 237
PspN4I GGNNCC 2 cut(s) 43, 247
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 2 cut(s) 272, 456
SalI GTCGAC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 347, 479
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 3 cut(s) 12, 41, 460
SchI GAGTC 2 cut(s) 149, 312
ScrFI CCNGG 3 cut(s) 133, 239, 383
SexAI ACCWGGT 1 cut(s) 131
SfaNI GCATC 3 cut(s) 16, 156, 292
SmiMI CAYNNNNRTG 2 cut(s) 272, 456
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 2 cut(s) 341, 445
SsiI CCGC 1 cut(s) 84
SspMI CTAG 3 cut(s) 159, 390, 547
StyD4I CCNGG 3 cut(s) 131, 237, 381
TaaI ACNGT 1 cut(s) 208
TaqI TCGA 1 cut(s) 257
TasI AATT 2 cut(s) 341, 445
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 2 cut(s) 347, 479
Tru9I TTAA 2 cut(s) 347, 479
TscAI CASTG 2 cut(s) 211, 279
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 440, 446
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 2 cut(s) 211, 279
XbaI TCTAGA 1 cut(s) 158
XceI RCATGY 1 cut(s) 288
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 3 cut(s) 159, 390, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.