MD11G1211900.v1.1

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003991
Physical Location & Seq
Forward (+)
30940886 .. 30944196
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1211900.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGACGAGGTAATCACTGCCGCTGATGCCTCATCAAATGGATCTACATCATCACAGCCGTCCATTCTTCCCTCCAATCTCCCCCTCCTCTCTGCCTTCCTCTCCTGCGCTCTCGCTCAGTTCCTCAAGCTCTTCACCACCTGGTACAAAGAGAAACGATGGGACTCCAGAAGAATGCTAGGGTCAGGGGGAATGCCCTCATCGCATTCGGCGACTGTGACGGCATTGGCAGTTGCTATTGGTTTCCAGGAAGGAACCGGATCCGCATTTGCCATTGCCGTGGTTTTGGCATGCGTTGTCATGTATGATGCAACTGGTGTAAGACTTCATGCTGGTCGTCAAGCTGAATTATTAAATCAAATAGTGTGTGAGCTACCTCCAGAACACCCTGTTTCTAGTGTTAGACCTCTGCGGGATTCTCTTGGTCATACTCCATTGCAGGTTCTTGCAGGTGCAGTGTTGGGATGTATTGTAGCTTATCTCATGAGAAGTTCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

167

Amino Acids

17.49

Weight (kDa)

6.89

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 25 - 158 1.3e-49 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 440
Acc36I ACCTGC 2 cut(s) 430, 440
AciI CCGC 3 cut(s) 21, 264, 412
AclWI GGATC 3 cut(s) 49, 255, 268
AfaI GTAC 1 cut(s) 146
AjnI CCWGG 2 cut(s) 140, 246
AluBI AGCT 5 cut(s) 130, 344, 373, 476, 498
AluI AGCT 5 cut(s) 130, 344, 373, 476, 498
AlwI GGATC 3 cut(s) 49, 255, 268
AspLEI GCGC 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 127
BamHI GGATCC 1 cut(s) 260
BccI CCATC 1 cut(s) 153
BceAI ACGGC 3 cut(s) 43, 237, 263
BciT130I CCWGG 2 cut(s) 142, 248
BfaI CTAG 3 cut(s) 179, 396, 499
BfuAI ACCTGC 2 cut(s) 430, 440
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 2 cut(s) 142, 248
BmiI GGNNCC 2 cut(s) 256, 262
BmrFI CCNGG 2 cut(s) 142, 248
BmsI GCATC 2 cut(s) 16, 298
BpmI CTGGAG 2 cut(s) 151, 363
BpuEI CTTGAG 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 279
BsaWI WCCGGW 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 2 cut(s) 273, 434
Bse8I GATNNNNATC 1 cut(s) 46
BseBI CCWGG 2 cut(s) 142, 248
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 1 cut(s) 470
BseJI GATNNNNATC 1 cut(s) 46
BseMI GCAATG 2 cut(s) 273, 434
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 319
BseRI GAGGAG 1 cut(s) 77
BsgI GTGCAG 1 cut(s) 474
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 176
BsmI GAATGC 3 cut(s) 180, 198, 205
Bsp143I GATC 2 cut(s) 41, 260
BspACI CCGC 3 cut(s) 21, 264, 412
BspCNI CTCAG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 2 cut(s) 256, 262
BspMI ACCTGC 2 cut(s) 430, 440
BspPI GGATC 3 cut(s) 49, 255, 268
BspQI GCTCTTC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 273, 434
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 2 cut(s) 41, 260
Bst2UI CCWGG 2 cut(s) 142, 248
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 292
BstDEI CTNAG 1 cut(s) 117
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 2 cut(s) 44, 263
BstMBI GATC 2 cut(s) 41, 260
BstMWI GCNNNNNNNGC 2 cut(s) 26, 202
BstNI CCWGG 2 cut(s) 142, 248
BstNSI RCATGY 1 cut(s) 294
BstSCI CCNGG 2 cut(s) 140, 246
BstX2I RGATCY 2 cut(s) 41, 260
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 2 cut(s) 41, 260
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 470
BtsI GCAGTG 2 cut(s) 15, 462
BtsIMutI CAGTG 2 cut(s) 15, 462
BveI ACCTGC 2 cut(s) 430, 440
Cac8I GCNNGC 1 cut(s) 292
CciI TCATGA 1 cut(s) 483
CfoI GCGC 1 cut(s) 110
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 1 cut(s) 145
CviAII CATG 4 cut(s) 291, 301, 329, 484
CviJI RGCY 6 cut(s) 58, 130, 344, 373, 476, 498
CviKI_1 RGCY 6 cut(s) 58, 130, 344, 373, 476, 498
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 1 cut(s) 117
DpnI GATC 2 cut(s) 43, 262
DpnII GATC 2 cut(s) 41, 260
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 140, 246
FaeI CATG 4 cut(s) 294, 304, 332, 487
FaiI YATR 6 cut(s) 292, 302, 306, 330, 429, 485
FaqI GGGAC 1 cut(s) 176
FatI CATG 4 cut(s) 290, 300, 328, 483
FauI CCCGC 1 cut(s) 405
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 477
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 3 cut(s) 179, 396, 499
GlaI GCGC 1 cut(s) 109
GluI GCNGC 1 cut(s) 21
GsuI CTGGAG 2 cut(s) 151, 363
HapII CCGG 1 cut(s) 258
HhaI GCGC 1 cut(s) 110
Hin1II CATG 4 cut(s) 294, 304, 332, 487
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 2 cut(s) 164, 416
HpaII CCGG 1 cut(s) 258
HphI GGTGA 1 cut(s) 127
Hpy188III TCNNGA 3 cut(s) 168, 380, 484
HpyAV CCTTC 2 cut(s) 106, 245
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 4 cut(s) 311, 439, 449, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 202
HpyF3I CTNAG 1 cut(s) 117
Hsp92II CATG 4 cut(s) 294, 304, 332, 487
HspAI GCGC 1 cut(s) 108
Kzo9I GATC 2 cut(s) 41, 260
LguI GCTCTTC 1 cut(s) 137
LweI GCATC 2 cut(s) 16, 298
MabI ACCWGGT 1 cut(s) 140
MaeI CTAG 3 cut(s) 179, 396, 499
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 43, 262
MboI GATC 2 cut(s) 41, 260
MboII GAAGA 3 cut(s) 59, 124, 183
MflI RGATCY 2 cut(s) 41, 260
MluCI AATT 1 cut(s) 347
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 9 cut(s) 40, 82, 95, 98, 110, 134, 208, 387, 417
MseI TTAA 1 cut(s) 353
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 2 cut(s) 142, 248
Mva1269I GAATGC 3 cut(s) 180, 198, 205
MvaI CCWGG 2 cut(s) 142, 248
MwoI GCNNNNNNNGC 2 cut(s) 26, 202
NdeII GATC 2 cut(s) 41, 260
NlaIII CATG 4 cut(s) 294, 304, 332, 487
NlaIV GGNNCC 2 cut(s) 256, 262
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 1 cut(s) 294
PaeI GCATGC 1 cut(s) 294
PagI TCATGA 1 cut(s) 483
PaqCI CACCTGC 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PctI GAATGC 3 cut(s) 180, 198, 205
PfeI GAWTC 1 cut(s) 416
PfoI TCCNGGA 1 cut(s) 246
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 140, 246
PspGI CCWGG 2 cut(s) 140, 246
PspN4I GGNNCC 2 cut(s) 256, 262
PsuI RGATCY 2 cut(s) 41, 260
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 278
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 2 cut(s) 41, 260
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 142, 248
SexAI ACCWGGT 1 cut(s) 140
SfaNI GCATC 2 cut(s) 16, 298
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SphI GCATGC 1 cut(s) 294
Sse9I AATT 1 cut(s) 347
SsiI CCGC 3 cut(s) 21, 264, 412
SspMI CTAG 3 cut(s) 179, 396, 499
StyD4I CCNGG 2 cut(s) 140, 246
TaaI ACNGT 1 cut(s) 217
TasI AATT 1 cut(s) 347
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TscAI CASTG 2 cut(s) 22, 462
TseFI GTSAC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 317
TspRI CASTG 2 cut(s) 22, 462
XceI RCATGY 1 cut(s) 294
XspI CTAG 3 cut(s) 179, 396, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.