pycom03g14770

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003991
Physical Location & Seq
Forward (+)
16080821 .. 16083183
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g14770.1

Sequence Viewer

Length: 504 bp
ATGGACGAGGTACTCACTGCTGCTGATGCCTCATCAAATGGATCTACATCATCACAGCCGTCCATTCTTCCCTATAATCTCCCCCTCCTCTCTGCCTTCCTGTCCTGCGCTCTTGCTCAGTTTCTCAAGCTCTTCACTACCTGGTACAAAGAGAAAAGATGGGATTCCAGAAGAATGCTTGGGTCAGGAGGAATGCCCTCATCGCATTCCTCAACTGTGACGGCGTTGGTAGTTGCTATTGGTTTACAGGAAGGAACCGGAGGGTCTGCATTTGCCATTGCCGTGGTTTTGGCATGTGTTGTCATGTATGATGCCACTGGTGTAAGACTTCATGCTGGTCGTCAAGCTGAATTATTAAATCAAATAGTGTGTGAGCTACCTCCAGAACACCCTGTTTCTAGTGTTAGACCTCTGCGGGATTCACTTGGTCATACTCCATTGCAGGTTCTTGCAGGTGCAGTGTTGGGATGTATAGTAGCGTATCTCATGAAAAGTTCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

168

Amino Acids

17.61

Weight (kDa)

6.89

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 26 - 159 4.4e-50 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 443
Acc36I ACCTGC 2 cut(s) 433, 443
AciI CCGC 1 cut(s) 415
AclWI GGATC 1 cut(s) 49
AfaI GTAC 2 cut(s) 12, 146
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 4 cut(s) 130, 347, 376, 501
AluI AGCT 4 cut(s) 130, 347, 376, 501
AlwI GGATC 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 110
BbvI GCAGC 1 cut(s) 7
BccI CCATC 1 cut(s) 153
BceAI ACGGC 3 cut(s) 43, 237, 266
BciT130I CCWGG 1 cut(s) 142
BfaI CTAG 2 cut(s) 399, 502
BfuAI ACCTGC 2 cut(s) 433, 443
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 16, 301
BpmI CTGGAG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 282
BsaWI WCCGGW 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 2 cut(s) 276, 437
Bse8I GATNNNNATC 1 cut(s) 46
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 473
BseJI GATNNNNATC 1 cut(s) 46
BseMI GCAATG 2 cut(s) 276, 437
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 1 cut(s) 77
BseXI GCAGC 1 cut(s) 7
BsgI GTGCAG 1 cut(s) 477
BsiSI CCGG 1 cut(s) 258
BsmI GAATGC 3 cut(s) 180, 198, 205
Bsp143I GATC 1 cut(s) 41
BspACI CCGC 1 cut(s) 415
BspCNI CTCAG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 256
BspMI ACCTGC 2 cut(s) 433, 443
BspPI GGATC 1 cut(s) 49
BspQI GCTCTTC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 276, 437
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 117
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 1 cut(s) 473
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 1 cut(s) 44
BstMBI GATC 1 cut(s) 41
BstMWI GCNNNNNNNGC 2 cut(s) 26, 202
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 140
BstV1I GCAGC 1 cut(s) 7
BstX2I RGATCY 1 cut(s) 41
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 473
BtsI GCAGTG 2 cut(s) 15, 465
BtsIMutI CAGTG 3 cut(s) 15, 315, 465
BveI ACCTGC 2 cut(s) 433, 443
CciI TCATGA 1 cut(s) 486
CfoI GCGC 1 cut(s) 110
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 2 cut(s) 11, 145
CviAII CATG 4 cut(s) 294, 304, 332, 487
CviJI RGCY 5 cut(s) 58, 130, 347, 376, 501
CviKI_1 RGCY 5 cut(s) 58, 130, 347, 376, 501
CviQI GTAC 2 cut(s) 11, 145
DdeI CTNAG 1 cut(s) 117
DpnI GATC 1 cut(s) 43
DpnII GATC 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 140
FaeI CATG 4 cut(s) 297, 307, 335, 490
FaiI YATR 8 cut(s) 75, 295, 305, 309, 333, 432, 473, 488
FatI CATG 4 cut(s) 293, 303, 331, 486
FauI CCCGC 1 cut(s) 408
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 480
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 2 cut(s) 399, 502
GlaI GCGC 1 cut(s) 109
GluI GCNGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 366
HapII CCGG 1 cut(s) 258
HhaI GCGC 1 cut(s) 110
Hin1II CATG 4 cut(s) 297, 307, 335, 490
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 2 cut(s) 164, 419
HpaII CCGG 1 cut(s) 258
Hpy166II GTNNAC 1 cut(s) 245
Hpy188III TCNNGA 4 cut(s) 168, 186, 383, 487
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 106, 245
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 4 cut(s) 269, 442, 452, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 202
HpyF3I CTNAG 1 cut(s) 117
Hsp92II CATG 4 cut(s) 297, 307, 335, 490
HspAI GCGC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 41
LguI GCTCTTC 1 cut(s) 137
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 2 cut(s) 16, 301
MabI ACCWGGT 1 cut(s) 140
MaeI CTAG 2 cut(s) 399, 502
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 43
MboI GATC 1 cut(s) 41
MboII GAAGA 3 cut(s) 59, 124, 183
MflI RGATCY 1 cut(s) 41
MluCI AATT 1 cut(s) 350
MnlI CCTC 9 cut(s) 40, 95, 98, 182, 208, 220, 254, 390, 420
MseI TTAA 1 cut(s) 356
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 142
Mva1269I GAATGC 3 cut(s) 180, 198, 205
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 26, 202
NdeII GATC 1 cut(s) 41
NlaIII CATG 4 cut(s) 297, 307, 335, 490
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 1 cut(s) 297
PagI TCATGA 1 cut(s) 486
PaqCI CACCTGC 1 cut(s) 443
PciSI GCTCTTC 1 cut(s) 137
PctI GAATGC 3 cut(s) 180, 198, 205
PfeI GAWTC 2 cut(s) 164, 419
PkrI GCNGC 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 256
PsuI RGATCY 1 cut(s) 41
RsaI GTAC 2 cut(s) 12, 146
RsaNI GTAC 2 cut(s) 11, 145
RseI CAYNNNNRTG 1 cut(s) 281
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 142
SexAI ACCWGGT 1 cut(s) 140
SfaNI GCATC 2 cut(s) 16, 301
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 1 cut(s) 350
SsiI CCGC 1 cut(s) 415
SspMI CTAG 2 cut(s) 399, 502
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 217
TasI AATT 1 cut(s) 350
TfiI GAWTC 2 cut(s) 164, 419
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TscAI CASTG 3 cut(s) 22, 322, 465
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 320, 503
TspRI CASTG 3 cut(s) 22, 322, 465
XceI RCATGY 1 cut(s) 297
XspI CTAG 2 cut(s) 399, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.