Prupe.3G213500_v2.0.a1

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0003991
Physical Location & Seq
Forward (+)
21734993 .. 21737103
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G213500.1

Sequence Viewer

Length: 489 bp
ATGACGGCCGCGGATGCGACGACGTCGTCTTCACCGTCGACGACTGCAACGATCCCGTCGAACCTCCCTCTCATCTCTGCGTTCCTCGCCTGCGCTCTCGCTCAGTTCCTTAAGCTCTTCACCTCCTGGTACAAAGAAAGGAGATGGGATTCGAAGCGAATGCTCGATTCCGGCGGAATGCCGTCGTCGCATTCGGCCACGGTGACGGCTCTGGCGGTCGCCATTGGCCTTCAAGATGGCGTCGGAGGACCCGCTTTCGCCATTGCTCTGGTCTTAGCATGCATTGTGATGTATGATGCCTCCGGAGTGAGACTTCATGCTGGTCGGCAAGCCGAGTTGCTGAACCAAATTGTGTACGAGCTTCCTCCGGAGCATCCTTTGTCCAGTGTTAGACCTCTGCGCGATTCACTTGGACATACTCCCTTGCAGGTTGTTGCTGGTGCCGTGCTGGGATGCGTAGTGGCATATCTAATGCGGAACACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

163

Amino Acids

17.11

Weight (kDa)

8.54

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 418
AccB1I GGYRCC 1 cut(s) 440
AccI GTMKAC 1 cut(s) 38
AccII CGCG 2 cut(s) 11, 402
AccIII TCCGGA 2 cut(s) 302, 367
AciI CCGC 6 cut(s) 9, 11, 174, 215, 252, 475
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 2 cut(s) 6, 195
AcyI GRCGYC 2 cut(s) 23, 240
AfaI GTAC 2 cut(s) 131, 356
AflII CTTAAG 1 cut(s) 110
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 1 cut(s) 125
AluBI AGCT 2 cut(s) 115, 361
AluI AGCT 2 cut(s) 115, 361
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 1 cut(s) 46
Aor13HI TCCGGA 2 cut(s) 302, 367
AoxI GGCC 3 cut(s) 6, 195, 226
AspLEI GCGC 2 cut(s) 95, 402
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 3 cut(s) 24, 112, 214
AsuII TTCGAA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 248
BanI GGYRCC 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 21
BccI CCATC 2 cut(s) 138, 230
BceAI ACGGC 4 cut(s) 21, 166, 222, 428
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 304
BfrI CTTAAG 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 418
BisI GCNGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 2 cut(s) 250, 442
BmrFI CCNGG 1 cut(s) 127
BmsI GCATC 4 cut(s) 4, 286, 382, 443
BpiI GAAGAC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 152
BsaHI GRCGYC 2 cut(s) 23, 240
BsaJI CCNNGG 2 cut(s) 9, 198
BsaWI WCCGGW 2 cut(s) 302, 367
Bse1I ACTGG 1 cut(s) 384
Bse3DI GCAATG 1 cut(s) 261
BseAI TCCGGA 2 cut(s) 302, 367
BseBI CCWGG 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 9, 198
BseGI GGATG 3 cut(s) 19, 373, 458
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 384
BseX3I CGGCCG 1 cut(s) 6
BseYI CCCAGC 1 cut(s) 448
Bsh1236I CGCG 2 cut(s) 11, 402
Bsh1285I CGRYCG 2 cut(s) 9, 219
BshFI GGCC 3 cut(s) 8, 197, 228
BshNI GGYRCC 1 cut(s) 440
BsiEI CGRYCG 2 cut(s) 9, 219
BsiSI CCGG 3 cut(s) 171, 303, 368
BsmAI GTCTC 1 cut(s) 304
BsmI GAATGC 3 cut(s) 165, 183, 190
BsnI GGCC 3 cut(s) 8, 197, 228
Bsp119I TTCGAA 1 cut(s) 152
Bsp13I TCCGGA 2 cut(s) 302, 367
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 6 cut(s) 9, 11, 174, 215, 252, 475
BspANI GGCC 3 cut(s) 8, 197, 228
BspCNI CTCAG 1 cut(s) 115
BspEI TCCGGA 2 cut(s) 302, 367
BspFNI CGCG 2 cut(s) 11, 402
BspLI GGNNCC 2 cut(s) 250, 442
BspMI ACCTGC 1 cut(s) 418
BspPI GGATC 1 cut(s) 46
BspQI GCTCTTC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 440
BspTI CTTAAG 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 9, 198
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 2 cut(s) 23, 240
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 2 cut(s) 36, 202
Bst6I CTCTTC 1 cut(s) 122
BstACI GRCGYC 2 cut(s) 23, 240
BstAFI CTTAAG 1 cut(s) 110
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 91, 280, 330
BstDEI CTNAG 2 cut(s) 102, 274
BstDSI CCRYGG 2 cut(s) 9, 198
BstF5I GGATG 3 cut(s) 19, 373, 458
BstFNI CGCG 2 cut(s) 11, 402
BstHHI GCGC 2 cut(s) 95, 402
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 51
BstMCI CGRYCG 2 cut(s) 9, 219
BstMWI GCNNNNNNNGC 3 cut(s) 14, 86, 187
BstNI CCWGG 1 cut(s) 127
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 125
BstUI CGCG 2 cut(s) 11, 402
BstV2I GAAGAC 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 268
BstZI CGGCCG 1 cut(s) 6
BsuRI GGCC 3 cut(s) 8, 197, 228
BtgI CCRYGG 2 cut(s) 9, 198
BtsCI GGATG 3 cut(s) 19, 373, 458
BtsIMutI CAGTG 1 cut(s) 391
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 3 cut(s) 91, 280, 330
CfoI GCGC 2 cut(s) 95, 402
Cfr13I GGNCC 1 cut(s) 248
Cfr42I CCGCGG 1 cut(s) 12
CseI GACGC 1 cut(s) 229
Csp6I GTAC 2 cut(s) 130, 355
CviAII CATG 2 cut(s) 279, 317
CviJI RGCY 7 cut(s) 8, 115, 197, 209, 228, 332, 361
CviKI_1 RGCY 7 cut(s) 8, 115, 197, 209, 228, 332, 361
CviQI GTAC 2 cut(s) 130, 355
DdeI CTNAG 2 cut(s) 102, 274
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 2 cut(s) 6, 195
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 189
EclXI CGGCCG 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 248
Eco52I CGGCCG 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 125
EcoT22I ATGCAT 1 cut(s) 284
FaeI CATG 2 cut(s) 282, 320
FaiI YATR 5 cut(s) 280, 294, 318, 417, 466
FatI CATG 2 cut(s) 278, 316
FauI CCCGC 1 cut(s) 259
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 1 cut(s) 9
FokI GGATG 3 cut(s) 26, 360, 465
Fsp4HI GCNGC 1 cut(s) 9
GlaI GCGC 2 cut(s) 94, 401
GluI GCNGC 1 cut(s) 9
GsaI CCCAGC 1 cut(s) 452
HaeIII GGCC 3 cut(s) 8, 197, 228
HapII CCGG 3 cut(s) 171, 303, 368
HgaI GACGC 1 cut(s) 229
HhaI GCGC 2 cut(s) 95, 402
Hin1I GRCGYC 2 cut(s) 23, 240
Hin1II CATG 2 cut(s) 282, 320
Hin6I GCGC 2 cut(s) 93, 400
HinP1I GCGC 2 cut(s) 93, 400
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 3 cut(s) 149, 167, 404
HpaII CCGG 3 cut(s) 171, 303, 368
HphI GGTGA 3 cut(s) 24, 112, 214
Hpy166II GTNNAC 2 cut(s) 39, 355
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 3 cut(s) 233, 303, 368
Hpy8I GTNNAC 2 cut(s) 39, 355
Hpy99I CGWCG 9 cut(s) 22, 25, 28, 40, 43, 61, 187, 190, 245
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 2 cut(s) 36, 202
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 47, 282, 427
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 86, 187
HpyF3I CTNAG 2 cut(s) 102, 274
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 2 cut(s) 23, 240
Hsp92II CATG 2 cut(s) 282, 320
HspAI GCGC 2 cut(s) 93, 400
Kpn2I TCCGGA 2 cut(s) 302, 367
KspI CCGCGG 1 cut(s) 12
Kzo9I GATC 1 cut(s) 51
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 1 cut(s) 370
LweI GCATC 4 cut(s) 4, 286, 382, 443
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 202
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 2 cut(s) 21, 109
MluCI AATT 1 cut(s) 348
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 8 cut(s) 74, 78, 95, 133, 239, 310, 375, 405
Mph1103I ATGCAT 1 cut(s) 284
MroI TCCGGA 2 cut(s) 302, 367
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 11
MspCI CTTAAG 1 cut(s) 110
MspI CCGG 3 cut(s) 171, 303, 368
MspR9I CCNGG 1 cut(s) 127
Mva1269I GAATGC 3 cut(s) 165, 183, 190
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 2 cut(s) 11, 402
MwoI GCNNNNNNNGC 3 cut(s) 14, 86, 187
NdeII GATC 1 cut(s) 51
NlaIII CATG 2 cut(s) 282, 320
NlaIV GGNNCC 2 cut(s) 250, 442
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 284
NspI RCATGY 1 cut(s) 282
NspV TTCGAA 1 cut(s) 152
PaeI GCATGC 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 3 cut(s) 32, 47, 56
PctI GAATGC 3 cut(s) 165, 183, 190
PfeI GAWTC 3 cut(s) 149, 167, 404
PflFI GACNNNGTC 2 cut(s) 22, 25
PkrI GCNGC 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 1 cut(s) 125
PspN4I GGNNCC 2 cut(s) 250, 442
PspPI GGNCC 1 cut(s) 248
PspPPI RGGWCCY 1 cut(s) 248
PsyI GACNNNGTC 2 cut(s) 22, 25
RsaI GTAC 2 cut(s) 131, 356
RsaNI GTAC 2 cut(s) 130, 355
RseI CAYNNNNRTG 1 cut(s) 287
SacII CCGCGG 1 cut(s) 12
SalI GTCGAC 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 127
SetI ASST 7 cut(s) 26, 66, 117, 125, 363, 397, 432
SfaNI GCATC 4 cut(s) 4, 286, 382, 443
Sfr303I CCGCGG 1 cut(s) 12
SfuI TTCGAA 1 cut(s) 152
SgrBI CCGCGG 1 cut(s) 12
SgrDI CGTCGACG 1 cut(s) 37
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SphI GCATGC 1 cut(s) 282
Sse9I AATT 1 cut(s) 348
SsiI CCGC 6 cut(s) 9, 11, 174, 215, 252, 475
StyD4I CCNGG 1 cut(s) 125
TaaI ACNGT 2 cut(s) 36, 202
TaiI ACGT 1 cut(s) 26
TaqI TCGA 4 cut(s) 38, 59, 152, 165
TasI AATT 1 cut(s) 348
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 3 cut(s) 149, 167, 404
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 305
TspRI CASTG 1 cut(s) 391
Tth111I GACNNNGTC 2 cut(s) 22, 25
Vha464I CTTAAG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 248
XceI RCATGY 1 cut(s) 282
XmiI GTMKAC 1 cut(s) 38
ZraI GACGTC 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.