pycom09g03490

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003991
Physical Location & Seq
Reverse (-)
2581345 .. 2582753
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g03490.1

Sequence Viewer

Length: 489 bp
ATGACGGCTGCGGATGCGACGACGTCGTCTTCGCCGTCGACGGCGGCGGCGATCCCATCGAACCTCGCTCTCCTCGCTGCGCTCCTAGCATGCGCTCTCGCTCAGTTCCTCAAGCTCTTCACCACCTGGTACAAAGAAAAGAGATGGGACTCGAAGAAAATGCTCGATTCCGGCGGGATGCCGTCGTCACATTCCGCCACGGTGACAGCTCTGGCCGTCGCCGTTGGCCTTAAGGACGGCACCGGAGGACCTGCCTTCGCCATTGCCTTTGTCTTAGCTTGCATTGTTATGTATGATGCCTCAGGTGTGAGACTTCATGCTGGTCGGCAAGCCGAGTTGCTGAACCAAATAGTGTGTGAGCTTCCTCCAGAGCATCCTTTGTCCAGCGTTAGACCTCTGCGCGATTCACTTGGACATACTCCGCTACAGGTTGCTGCAGGTGCTGTGTTGGGATGCATAGTAGCTTACCTAATGAGTAACACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

163

Amino Acids

16.81

Weight (kDa)

8.4

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 21 - 154 2.3e-50 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 428
AasI GACNNNNNNGTC 1 cut(s) 25
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 2 cut(s) 259, 428
AccB1I GGYRCC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 38
AccII CGCG 1 cut(s) 402
AciI CCGC 6 cut(s) 11, 44, 47, 174, 195, 422
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 131
AflII CTTAAG 1 cut(s) 230
AjnI CCWGG 1 cut(s) 125
AluBI AGCT 5 cut(s) 115, 209, 278, 361, 464
AluI AGCT 5 cut(s) 115, 209, 278, 361, 464
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 1 cut(s) 46
AlwNI CAGNNNCTG 1 cut(s) 443
AoxI GGCC 2 cut(s) 213, 226
ApeKI GCWGC 3 cut(s) 8, 77, 434
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
AspLEI GCGC 3 cut(s) 82, 95, 402
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 112, 214
AvaII GGWCC 1 cut(s) 248
AxyI CCTNAGG 1 cut(s) 301
BanI GGYRCC 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 2 cut(s) 64, 421
BccI CCATC 2 cut(s) 64, 138
BceAI ACGGC 7 cut(s) 19, 21, 57, 166, 200, 206, 253
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 1 cut(s) 86
BfmI CTRYAG 2 cut(s) 425, 435
BfrI CTTAAG 1 cut(s) 230
BfuAI ACCTGC 2 cut(s) 259, 428
BisI GCNGC 5 cut(s) 9, 45, 48, 78, 435
BlsI GCNGC 5 cut(s) 10, 46, 49, 79, 436
Bme1390I CCNGG 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 1 cut(s) 127
BmsI GCATC 5 cut(s) 4, 168, 286, 382, 443
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 1 cut(s) 242
Bse21I CCTNAGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 127
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 4 cut(s) 19, 183, 373, 458
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 116, 315
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 64, 421
Bsh1236I CGCG 1 cut(s) 402
BshFI GGCC 2 cut(s) 215, 228
BshNI GGYRCC 1 cut(s) 239
BsiSI CCGG 2 cut(s) 171, 243
BslFI GGGAC 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 2 cut(s) 215, 228
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 6 cut(s) 11, 44, 47, 174, 195, 422
BspANI GGCC 2 cut(s) 215, 228
BspCNI CTCAG 2 cut(s) 115, 314
BspFNI CGCG 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 439
BspMI ACCTGC 2 cut(s) 259, 428
BspPI GGATC 1 cut(s) 46
BspQI GCTCTTC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 239
BspTI CTTAAG 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 261
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 1 cut(s) 122
BstACI GRCGYC 1 cut(s) 23
BstAFI CTTAAG 1 cut(s) 230
BstC8I GCNNGC 3 cut(s) 91, 280, 330
BstDEI CTNAG 3 cut(s) 102, 274, 301
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 4 cut(s) 19, 183, 373, 458
BstFNI CGCG 1 cut(s) 402
BstHHI GCGC 3 cut(s) 82, 95, 402
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 4 cut(s) 14, 74, 86, 440
BstNI CCWGG 1 cut(s) 127
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 125
BstSFI CTRYAG 2 cut(s) 425, 435
BstUI CGCG 1 cut(s) 402
BstV1I GCAGC 2 cut(s) 64, 421
BstV2I GAAGAC 1 cut(s) 21
Bsu36I CCTNAGG 1 cut(s) 301
BsuRI GGCC 2 cut(s) 215, 228
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 4 cut(s) 19, 183, 373, 458
BveI ACCTGC 2 cut(s) 259, 428
Cac8I GCNNGC 3 cut(s) 91, 280, 330
CaiI CAGNNNCTG 1 cut(s) 443
CfoI GCGC 3 cut(s) 82, 95, 402
Cfr13I GGNCC 1 cut(s) 248
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 1 cut(s) 130
CviAII CATG 2 cut(s) 90, 317
CviJI RGCY 9 cut(s) 8, 115, 209, 215, 228, 278, 332, 361, 464
CviKI_1 RGCY 9 cut(s) 8, 115, 209, 215, 228, 278, 332, 361, 464
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 3 cut(s) 102, 274, 301
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 248
Eco81I CCTNAGG 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 125
EcoT22I ATGCAT 1 cut(s) 458
FaeI CATG 2 cut(s) 93, 320
FaiI YATR 6 cut(s) 91, 290, 294, 318, 417, 458
FaqI GGGAC 1 cut(s) 161
FatI CATG 2 cut(s) 89, 316
FauI CCCGC 1 cut(s) 167
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 5 cut(s) 9, 45, 48, 78, 435
FokI GGATG 4 cut(s) 26, 190, 360, 465
Fsp4HI GCNGC 5 cut(s) 9, 45, 48, 78, 435
FspBI CTAG 1 cut(s) 86
GlaI GCGC 3 cut(s) 81, 94, 401
GluI GCNGC 5 cut(s) 9, 45, 48, 78, 435
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 2 cut(s) 215, 228
HapII CCGG 2 cut(s) 171, 243
HhaI GCGC 3 cut(s) 82, 95, 402
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 2 cut(s) 93, 320
Hin6I GCGC 3 cut(s) 80, 93, 400
HinP1I GCGC 3 cut(s) 80, 93, 400
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 3 cut(s) 149, 167, 404
HpaII CCGG 2 cut(s) 171, 243
HphI GGTGA 2 cut(s) 112, 214
Hpy166II GTNNAC 1 cut(s) 39
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 1 cut(s) 39
Hpy99I CGWCG 7 cut(s) 22, 25, 28, 40, 43, 187, 221
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 282, 437, 456
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 74, 86, 440
HpyF3I CTNAG 3 cut(s) 102, 274, 301
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 2 cut(s) 93, 320
HspAI GCGC 3 cut(s) 80, 93, 400
Kzo9I GATC 1 cut(s) 51
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 1 cut(s) 87
Lsp1109I GCAGC 2 cut(s) 64, 421
LweI GCATC 5 cut(s) 4, 168, 286, 382, 443
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 86
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 3 cut(s) 186, 202, 476
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 3 cut(s) 21, 109, 166
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 7 cut(s) 74, 83, 119, 239, 310, 375, 405
Mph1103I ATGCAT 1 cut(s) 458
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 287
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 2 cut(s) 171, 243
MspR9I CCNGG 1 cut(s) 127
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 1 cut(s) 402
MwoI GCNNNNNNNGC 4 cut(s) 14, 74, 86, 440
NdeII GATC 1 cut(s) 51
NlaIII CATG 2 cut(s) 93, 320
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 2 cut(s) 186, 202
NsiI ATGCAT 1 cut(s) 458
NspI RCATGY 1 cut(s) 93
PaeI GCATGC 1 cut(s) 93
PaqCI CACCTGC 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 47
PfeI GAWTC 2 cut(s) 167, 404
PflFI GACNNNGTC 2 cut(s) 22, 25
PkrI GCNGC 5 cut(s) 10, 46, 49, 79, 436
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 125
PspGI CCWGG 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 248
PspPPI RGGWCCY 1 cut(s) 248
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 443
PsyI GACNNNGTC 2 cut(s) 22, 25
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
RseI CAYNNNNRTG 1 cut(s) 287
SalI GTCGAC 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 5 cut(s) 9, 45, 48, 78, 435
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 127
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 5 cut(s) 4, 168, 286, 382, 443
SfcI CTRYAG 2 cut(s) 425, 435
SgrDI CGTCGACG 1 cut(s) 37
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 2 cut(s) 110, 230
SmoI CTYRAG 2 cut(s) 110, 230
SphI GCATGC 1 cut(s) 93
SsiI CCGC 6 cut(s) 11, 44, 47, 174, 195, 422
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 125
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 26
TaqI TCGA 4 cut(s) 38, 59, 152, 165
TauI GCSGC 2 cut(s) 47, 50
TfiI GAWTC 2 cut(s) 167, 404
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseFI GTSAC 2 cut(s) 186, 202
TseI GCWGC 3 cut(s) 8, 77, 434
Tsp45I GTSAC 2 cut(s) 186, 202
TspDTI ATGAA 1 cut(s) 305
Tth111I GACNNNGTC 2 cut(s) 22, 25
Vha464I CTTAAG 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 248
XceI RCATGY 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 1 cut(s) 86
ZraI GACGTC 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.