MD09G1072600.v1.1

protein C9orf85 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006795
Physical Location & Seq
Forward (+)
5049972 .. 5051757
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1072600.v1.1.491

Sequence Viewer

Length: 561 bp
ATGATGAACGGCGGTCGGCACCAAAACAAATTCGCATGGAAGCCCAACCTCGGCGTCAAAATCAACGAAACCGAGGTCGGAGGCCGGTTCCAGCCGCTGTCGGAAATCACGGGGGTCTGCCAACGCTGCAAGGAACAGATCGAGTGGAAGCGCCGTTACGGAAAGTACAAGACCCTGACGGAGCCCGCCAAGTGCCAACGCTGTACCAAACGCGCCGTCCGGCAGTCCCACCATAAGCTCTGCCCTGCTTGCGCCAAGGAACAAGGTGTTTGTGCAAGTGCTGCTGCCGTGTCGAGCAAATCGTCGGAAAAGACCTCGCGGAAGTGGAGGCTGAGCAGAAGAAGCTTCAAGAGGCGATCAAAAATGCAAGAGAAAGAGATAGGAGGAGTCTGTTGCGTGCGGTATATGAATGCTGGAAAATCTGCGGGTGTAGCGAAAGCTGCAGGCGAGAAAGGAGGCAAAGCGGGGGACTTGTTCCCCTCCGCGTCGCTTGAAGAATATGCAGCGGCAACCAGAGACGATGATGATGATGTGGAGGAGGATGGTGGAGGAGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2039

Protein Analysis

187

Amino Acids

20.54

Weight (kDa)

9.58

Isoelectric Point (pI)

68.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2039 PF10217 6 - 93 1e-26 Uncharacterized conserved protein (DUF2039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AccII CGCG 3 cut(s) 213, 319, 485
AciI CCGC 9 cut(s) 12, 95, 186, 319, 400, 425, 464, 483, 506
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 167, 205
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 2 cut(s) 349, 494
AluBI AGCT 3 cut(s) 238, 345, 440
AluI AGCT 3 cut(s) 238, 345, 440
Alw26I GTCTC 1 cut(s) 510
AlwNI CAGNNNCTG 1 cut(s) 97
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 5 cut(s) 126, 281, 284, 440, 503
ApoI RAATTY 1 cut(s) 29
ArsI GACNNNNNNTTYG 4 cut(s) 60, 92, 201, 233
AspLEI GCGC 3 cut(s) 153, 215, 254
BanI GGYRCC 1 cut(s) 18
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbvI GCAGC 5 cut(s) 113, 268, 271, 427, 515
BccI CCATC 2 cut(s) 536, 549
BceAI ACGGC 4 cut(s) 25, 138, 200, 272
BcoDI GTCTC 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 441
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 7 cut(s) 95, 127, 282, 285, 441, 504, 507
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 7 cut(s) 96, 128, 283, 286, 442, 505, 508
BmiI GGNNCC 3 cut(s) 20, 89, 183
Bpu1102I GCTNAGC 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 49, 72, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 84
BseDI CCNNGG 3 cut(s) 49, 72, 255
BseGI GGATG 2 cut(s) 547, 560
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 323
BseRI GAGGAG 2 cut(s) 399, 551
BseXI GCAGC 5 cut(s) 113, 268, 271, 427, 515
Bsh1236I CGCG 3 cut(s) 213, 319, 485
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 84
BshNI GGYRCC 1 cut(s) 18
BsiEI CGRYCG 1 cut(s) 16
BsiSI CCGG 2 cut(s) 85, 220
BslFI GGGAC 2 cut(s) 211, 482
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 1 cut(s) 510
BsmBI CGTCTC 1 cut(s) 510
BsmFI GGGAC 2 cut(s) 211, 482
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 138, 356
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 9 cut(s) 12, 95, 186, 319, 400, 425, 464, 483, 506
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 324
BspFNI CGCG 3 cut(s) 213, 319, 485
BspLI GGNNCC 3 cut(s) 20, 89, 183
BspMAI CTGCAG 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 3 cut(s) 49, 72, 255
BssMI GATC 2 cut(s) 138, 356
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 255
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 281
BstC8I GCNNGC 4 cut(s) 186, 250, 398, 445
BstDEI CTNAG 1 cut(s) 332
BstF5I GGATG 2 cut(s) 547, 560
BstFNI CGCG 3 cut(s) 213, 319, 485
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 3 cut(s) 153, 215, 254
BstKTI GATC 2 cut(s) 141, 359
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 2 cut(s) 138, 356
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 6 cut(s) 126, 249, 281, 342, 431, 440
BstSFI CTRYAG 1 cut(s) 441
BstUI CGCG 3 cut(s) 213, 319, 485
BstV1I GCAGC 5 cut(s) 113, 268, 271, 427, 515
BsuRI GGCC 1 cut(s) 84
BtsCI GGATG 2 cut(s) 547, 560
Cac8I GCNNGC 4 cut(s) 186, 250, 398, 445
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 3 cut(s) 153, 215, 254
Cfr10I RCCGGY 1 cut(s) 84
CseI GACGC 2 cut(s) 43, 474
Csp6I GTAC 2 cut(s) 166, 204
CviAII CATG 1 cut(s) 36
CviJI RGCY 8 cut(s) 43, 84, 94, 184, 238, 331, 345, 440
CviKI_1 RGCY 8 cut(s) 43, 84, 94, 184, 238, 331, 345, 440
CviQI GTAC 2 cut(s) 166, 204
DdeI CTNAG 1 cut(s) 332
DpnI GATC 2 cut(s) 140, 358
DpnII GATC 2 cut(s) 138, 356
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 1 cut(s) 39
FaiI YATR 5 cut(s) 37, 234, 405, 407, 501
FaqI GGGAC 2 cut(s) 211, 482
FatI CATG 1 cut(s) 35
FauI CCCGC 3 cut(s) 193, 418, 457
Fnu4HI GCNGC 7 cut(s) 95, 127, 282, 285, 441, 504, 507
FokI GGATG 1 cut(s) 554
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 7 cut(s) 95, 127, 282, 285, 441, 504, 507
GlaI GCGC 3 cut(s) 152, 214, 253
GluI GCNGC 7 cut(s) 95, 127, 282, 285, 441, 504, 507
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 1 cut(s) 84
HapII CCGG 2 cut(s) 85, 220
HgaI GACGC 2 cut(s) 43, 474
HhaI GCGC 3 cut(s) 153, 215, 254
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 1 cut(s) 39
Hin6I GCGC 3 cut(s) 151, 213, 252
HinP1I GCGC 3 cut(s) 151, 213, 252
HindIII AAGCTT 1 cut(s) 343
HinfI GANTC 1 cut(s) 387
HpaII CCGG 2 cut(s) 85, 220
Hpy188I TCNGA 3 cut(s) 80, 103, 307
Hpy188III TCNNGA 1 cut(s) 349
Hpy99I CGWCG 2 cut(s) 307, 490
HpyCH4V TGCA 5 cut(s) 129, 275, 367, 443, 503
HpyF10VI GCNNNNNNNGC 6 cut(s) 126, 249, 281, 342, 431, 440
HpyF3I CTNAG 1 cut(s) 332
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 1 cut(s) 39
HspAI GCGC 3 cut(s) 151, 213, 252
Kzo9I GATC 2 cut(s) 138, 356
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 8 cut(s) 98, 104, 188, 233, 258, 399, 429, 526
Lsp1109I GCAGC 5 cut(s) 113, 268, 271, 427, 515
MaeIII GTNAC 1 cut(s) 155
MalI GATC 2 cut(s) 140, 358
MboI GATC 2 cut(s) 138, 356
MboII GAAGA 2 cut(s) 351, 506
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 1 cut(s) 29
MlyI GAGTC 1 cut(s) 396
MmeI TCCRAC 3 cut(s) 58, 81, 285
MspA1I CMGCKG 2 cut(s) 97, 506
MspI CCGG 2 cut(s) 85, 220
Mva1269I GAATGC 1 cut(s) 415
MvnI CGCG 3 cut(s) 213, 319, 485
MwoI GCNNNNNNNGC 6 cut(s) 126, 249, 281, 342, 431, 440
NdeII GATC 2 cut(s) 138, 356
NlaIII CATG 1 cut(s) 39
NlaIV GGNNCC 3 cut(s) 20, 89, 183
NmeAIII GCCGAG 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PctI GAATGC 1 cut(s) 415
PkrI GCNGC 7 cut(s) 96, 128, 283, 286, 442, 505, 508
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
PspN4I GGNNCC 3 cut(s) 20, 89, 183
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 97
RsaI GTAC 2 cut(s) 167, 205
RsaNI GTAC 2 cut(s) 166, 204
SatI GCNGC 7 cut(s) 95, 127, 282, 285, 441, 504, 507
Sau3AI GATC 2 cut(s) 138, 356
SchI GAGTC 1 cut(s) 396
SduI GDGCHC 1 cut(s) 186
SetI ASST 7 cut(s) 51, 78, 240, 268, 317, 347, 442
SfcI CTRYAG 1 cut(s) 441
Sse9I AATT 1 cut(s) 29
SsiI CCGC 9 cut(s) 12, 95, 186, 319, 400, 425, 464, 483, 506
StyI CCWWGG 1 cut(s) 255
TaqI TCGA 2 cut(s) 141, 293
TasI AATT 1 cut(s) 29
TatI WGTACW 1 cut(s) 165
TauI GCSGC 2 cut(s) 97, 509
TseI GCWGC 5 cut(s) 126, 281, 284, 440, 503
TspDTI ATGAA 2 cut(s) 20, 422
TspGWI ACGGA 2 cut(s) 174, 194
XapI RAATTY 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.