MD17G1063700.v1.1

protein C9orf85 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006795
Physical Location & Seq
Forward (+)
5193683 .. 5194653
971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1063700.v1.1.491

Sequence Viewer

Length: 591 bp
ATGATGAACGGCGGTCGGCACCAAAACAAATTCGCATGGAAGCCAAACCTCGGCGTCAAAATCAACGAAACCGAGGTCGGAGGCCGGTTCCGGCCGCTGTCGGAGATAACGGGGGTCTGCCAGCGCTGCAAGGAACAGATCGAGTGGAAGCGCCGTTACGGAAAGTACAAGACCCTGACGGAGCCCGCCAAGTGCCAACGTTGTACCAAACGAGCCGTCCGTCAGTCCCACCATAAGCTCTGTGCTGCTTGTGCTAAGGAACAACGTGTTTGTGCGAAGTGTTGCTGTCGTGTCGAGCAAATTATCGGAAAAGACCTTGCGGAAGTGGAGTCTGAGCAGAAGCAGCTTCAAGAGGCGATCAAAAATGCAAGAGAAAGAGATAGGAGGAGTCTGTTGCGTGCGATGAATGCTGGAAAATCTACGGGTGTAGCGAAAGCTGCAAGCGAGAAAGGAGACAAAGCGGGGGACCTGTTCCCGTCTGCGTCACTCGAAGAATATGCAGAGGCAAGCAGAGATGATGAGGATGACGAAGGTGATGATGGTGAAGGCGAAGAAGATGAGGCACATGTTTGCAATCAAAAGAGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2039

Protein Analysis

197

Amino Acids

22.12

Weight (kDa)

8.45

Isoelectric Point (pI)

54.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2039 PF10217 6 - 96 5.5e-27 Uncharacterized conserved protein (DUF2039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AciI CCGC 5 cut(s) 12, 95, 186, 320, 461
AclI AACGTT 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 167, 205
AfeI AGCGCT 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 50
AflIII ACRYGT 2 cut(s) 265, 565
AgsI TTSAA 1 cut(s) 350
AluBI AGCT 3 cut(s) 238, 346, 437
AluI AGCT 3 cut(s) 238, 346, 437
Alw26I GTCTC 1 cut(s) 447
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 2 cut(s) 82, 92
ApeKI GCWGC 4 cut(s) 126, 245, 343, 437
ApoI RAATTY 1 cut(s) 29
ArsI GACNNNNNNTTYG 4 cut(s) 60, 92, 201, 233
AspLEI GCGC 2 cut(s) 126, 153
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 2 cut(s) 545, 554
AvaII GGWCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 18
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbvI GCAGC 4 cut(s) 113, 232, 355, 424
BccI CCATC 1 cut(s) 533
BceAI ACGGC 3 cut(s) 25, 138, 200
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BcoDI GTCTC 1 cut(s) 447
BfoI RGCGCY 2 cut(s) 127, 154
BisI GCNGC 5 cut(s) 95, 127, 246, 344, 438
BlsI GCNGC 5 cut(s) 96, 128, 247, 345, 439
Bme18I GGWCC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 4 cut(s) 20, 89, 183, 467
Bpu10I CCTNAGC 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 49, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 49, 72
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 400
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 4 cut(s) 113, 232, 355, 424
Bsh1285I CGRYCG 2 cut(s) 16, 95
BshFI GGCC 2 cut(s) 84, 94
BshNI GGYRCC 1 cut(s) 18
BsiEI CGRYCG 2 cut(s) 16, 95
BsiSI CCGG 2 cut(s) 85, 91
BslFI GGGAC 2 cut(s) 211, 479
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 1 cut(s) 447
BsmFI GGGAC 2 cut(s) 211, 479
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 2 cut(s) 84, 94
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 138, 357
BspACI CCGC 5 cut(s) 12, 95, 186, 320, 461
BspANI GGCC 2 cut(s) 84, 94
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 4 cut(s) 20, 89, 183, 467
BspT107I GGYRCC 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 2 cut(s) 49, 72
BssMI GATC 2 cut(s) 138, 357
BssNI GRCGYC 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 54
BstC8I GCNNGC 5 cut(s) 122, 186, 399, 442, 508
BstDEI CTNAG 2 cut(s) 255, 333
BstF5I GGATG 1 cut(s) 529
BstH2I RGCGCY 2 cut(s) 127, 154
BstHHI GCGC 2 cut(s) 126, 153
BstKTI GATC 2 cut(s) 141, 360
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 2 cut(s) 138, 357
BstMCI CGRYCG 2 cut(s) 16, 95
BstMWI GCNNNNNNNGC 5 cut(s) 126, 251, 343, 407, 437
BstNSI RCATGY 1 cut(s) 569
BstV1I GCAGC 4 cut(s) 113, 232, 355, 424
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 2 cut(s) 84, 94
BtgZI GCGATG 1 cut(s) 416
BtsCI GGATG 1 cut(s) 529
Cac8I GCNNGC 5 cut(s) 122, 186, 399, 442, 508
CfoI GCGC 2 cut(s) 126, 153
Cfr10I RCCGGY 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 2 cut(s) 43, 471
Csp6I GTAC 2 cut(s) 166, 204
CviAII CATG 2 cut(s) 36, 566
CviJI RGCY 8 cut(s) 43, 84, 94, 184, 215, 238, 346, 437
CviKI_1 RGCY 8 cut(s) 43, 84, 94, 184, 215, 238, 346, 437
CviQI GTAC 2 cut(s) 166, 204
DdeI CTNAG 2 cut(s) 255, 333
DpnI GATC 2 cut(s) 140, 359
DpnII GATC 2 cut(s) 138, 357
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 466
Eco47III AGCGCT 1 cut(s) 125
Eco52I CGGCCG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 2 cut(s) 39, 569
FaiI YATR 4 cut(s) 37, 234, 498, 567
FaqI GGGAC 2 cut(s) 211, 479
FatI CATG 2 cut(s) 35, 565
FauI CCCGC 2 cut(s) 193, 454
Fnu4HI GCNGC 5 cut(s) 95, 127, 246, 344, 438
FokI GGATG 1 cut(s) 536
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 5 cut(s) 95, 127, 246, 344, 438
GlaI GCGC 2 cut(s) 125, 152
GluI GCNGC 5 cut(s) 95, 127, 246, 344, 438
HaeII RGCGCY 2 cut(s) 127, 154
HaeIII GGCC 2 cut(s) 84, 94
HapII CCGG 2 cut(s) 85, 91
HgaI GACGC 2 cut(s) 43, 471
HhaI GCGC 2 cut(s) 126, 153
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 39, 569
Hin6I GCGC 2 cut(s) 124, 151
HinP1I GCGC 2 cut(s) 124, 151
HinfI GANTC 2 cut(s) 329, 388
HpaII CCGG 2 cut(s) 85, 91
HphI GGTGA 2 cut(s) 545, 554
Hpy188I TCNGA 4 cut(s) 80, 103, 308, 334
Hpy188III TCNNGA 1 cut(s) 350
HpyAV CCTTC 2 cut(s) 524, 539
HpyCH4IV ACGT 2 cut(s) 199, 265
HpyCH4V TGCA 5 cut(s) 129, 368, 440, 500, 573
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 251, 343, 407, 437
HpyF3I CTNAG 2 cut(s) 255, 333
HpySE526I ACGT 2 cut(s) 199, 265
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 39, 569
HspAI GCGC 2 cut(s) 124, 151
Kzo9I GATC 2 cut(s) 138, 357
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 6 cut(s) 98, 104, 134, 188, 396, 482
Lsp1109I GCAGC 4 cut(s) 113, 232, 355, 424
MaeII ACGT 2 cut(s) 199, 265
MaeIII GTNAC 2 cut(s) 155, 483
MalI GATC 2 cut(s) 140, 359
MboI GATC 2 cut(s) 138, 357
MboII GAAGA 3 cut(s) 503, 563, 566
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 2 cut(s) 29, 300
MlyI GAGTC 2 cut(s) 338, 397
MmeI TCCRAC 2 cut(s) 58, 81
MnlI CCTC 9 cut(s) 59, 67, 74, 346, 378, 496, 514, 553, 576
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 2 cut(s) 85, 91
Mva1269I GAATGC 1 cut(s) 412
MwoI GCNNNNNNNGC 5 cut(s) 126, 251, 343, 407, 437
NdeII GATC 2 cut(s) 138, 357
NlaIII CATG 2 cut(s) 39, 569
NlaIV GGNNCC 4 cut(s) 20, 89, 183, 467
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 483
NspI RCATGY 1 cut(s) 569
PciI ACATGT 1 cut(s) 565
PcsI WCGNNNNNNNCGW 2 cut(s) 217, 428
PctI GAATGC 1 cut(s) 412
PkrI GCNGC 5 cut(s) 96, 128, 247, 345, 439
PleI GAGTC 2 cut(s) 337, 396
PpsI GAGTC 2 cut(s) 337, 396
PpuMI RGGWCCY 1 cut(s) 466
PscI ACATGT 1 cut(s) 565
Psp1406I AACGTT 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 466
PspN4I GGNNCC 4 cut(s) 20, 89, 183, 467
PspPI GGNCC 1 cut(s) 466
PspPPI RGGWCCY 1 cut(s) 466
RsaI GTAC 2 cut(s) 167, 205
RsaNI GTAC 2 cut(s) 166, 204
SatI GCNGC 5 cut(s) 95, 127, 246, 344, 438
Sau3AI GATC 2 cut(s) 138, 357
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 2 cut(s) 338, 397
SduI GDGCHC 1 cut(s) 186
SinI GGWCC 1 cut(s) 466
Sse9I AATT 2 cut(s) 29, 300
SsiI CCGC 5 cut(s) 12, 95, 186, 320, 461
TaiI ACGT 2 cut(s) 202, 268
TaqI TCGA 3 cut(s) 141, 294, 489
TasI AATT 2 cut(s) 29, 300
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 97
TseFI GTSAC 1 cut(s) 483
TseI GCWGC 4 cut(s) 126, 245, 343, 437
Tsp45I GTSAC 1 cut(s) 483
TspDTI ATGAA 2 cut(s) 20, 419
TspGWI ACGGA 3 cut(s) 174, 194, 209
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 1 cut(s) 29
XceI RCATGY 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.