MD17G1063100.v1.1

protein C9orf85 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006795
Physical Location & Seq
Forward (+)
5162313 .. 5163973
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1063100.v1.1.491

Sequence Viewer

Length: 606 bp
ATGATGAACGGCGGTCGGCACCAAAACAAATTCGCATGGAAGCCAAACCTCGGCGTCAAAATCAACGAAACCGAGGTCGGAGGCCGGTTCCGGCCGCTGTCGGAGATAACGGGGGTCTGCCAGCGGTGCAAGGAACAGATCGAGTGGAAGCGCCGTTACGGGAAGTACAAGACCCTGACGGAGCCCGCCAAGTGCCAACGTTGTACCAAACGCGCCGTCCGTCAGTCCCACCATAAGCTCTGTGCTGCTTGTGCTAAGGAACAGCGTGTTTGTGCGAAGTGTTGCTGTCGTGTCGAGCAAATTGTCGGAAAAGATCTTGCGGAAGTGGAGTCTGAGCAGTGTTGTACCAACATTCCTAACAAGAAGAAGCAGCTTCAAGAGGCGATCAAAAATGCAAGAGAAAAAGATAGGAGGAGTCTGTTGCGTGCGATGAATGCTGGAAAATCTACGGGTGTAGCGAAAGCTGCAAGCGAGAAAGGAGACAAAGCGGGGGACCTGTTCCCGTCTGTGTCACTCGAAGAATATGCAGAGGCAAGCAGAGATGATGAGGATGACGAAGGTGATGATGGTGAAGGCGAAGAAGATGAGGCACTGGTTTGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2039

Protein Analysis

202

Amino Acids

22.53

Weight (kDa)

8.37

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2039 PF10217 6 - 96 5.9e-27 Uncharacterized conserved protein (DUF2039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AccII CGCG 1 cut(s) 213
AciI CCGC 6 cut(s) 12, 95, 124, 186, 320, 488
AclI AACGTT 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 3 cut(s) 167, 205, 346
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 1 cut(s) 377
AluBI AGCT 3 cut(s) 238, 373, 464
AluI AGCT 3 cut(s) 238, 373, 464
Alw26I GTCTC 1 cut(s) 474
AoxI GGCC 2 cut(s) 82, 92
ApeKI GCWGC 3 cut(s) 245, 370, 464
ApoI RAATTY 1 cut(s) 29
ArsI GACNNNNNNTTYG 4 cut(s) 60, 92, 201, 233
AspLEI GCGC 2 cut(s) 153, 215
AspS9I GGNCC 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 572, 581
AvaII GGWCC 1 cut(s) 493
BanI GGYRCC 1 cut(s) 18
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbvI GCAGC 3 cut(s) 232, 382, 451
BccI CCATC 1 cut(s) 560
BceAI ACGGC 3 cut(s) 25, 138, 200
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BcoDI GTCTC 1 cut(s) 474
BfoI RGCGCY 1 cut(s) 154
BglII AGATCT 1 cut(s) 313
BisI GCNGC 4 cut(s) 95, 246, 371, 465
BlsI GCNGC 4 cut(s) 96, 247, 372, 466
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 4 cut(s) 20, 89, 183, 494
Bpu10I CCTNAGC 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 49, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 84
Bse1I ACTGG 1 cut(s) 597
BseDI CCNNGG 2 cut(s) 49, 72
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 1 cut(s) 427
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 3 cut(s) 232, 382, 451
Bsh1236I CGCG 1 cut(s) 213
Bsh1285I CGRYCG 2 cut(s) 16, 95
BshFI GGCC 2 cut(s) 84, 94
BshNI GGYRCC 1 cut(s) 18
BsiEI CGRYCG 2 cut(s) 16, 95
BsiSI CCGG 2 cut(s) 85, 91
BslFI GGGAC 2 cut(s) 211, 506
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 1 cut(s) 474
BsmFI GGGAC 2 cut(s) 211, 506
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 84, 94
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 138, 313, 384
BspACI CCGC 6 cut(s) 12, 95, 124, 186, 320, 488
BspANI GGCC 2 cut(s) 84, 94
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 4 cut(s) 20, 89, 183, 494
BspT107I GGYRCC 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 84
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 2 cut(s) 49, 72
BssMI GATC 3 cut(s) 138, 313, 384
BssNI GRCGYC 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 54
BstC8I GCNNGC 5 cut(s) 122, 186, 426, 469, 535
BstDEI CTNAG 2 cut(s) 255, 333
BstF5I GGATG 1 cut(s) 556
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 2 cut(s) 153, 215
BstKTI GATC 3 cut(s) 141, 316, 387
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 3 cut(s) 138, 313, 384
BstMCI CGRYCG 2 cut(s) 16, 95
BstMWI GCNNNNNNNGC 4 cut(s) 126, 251, 434, 464
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 3 cut(s) 232, 382, 451
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 2 cut(s) 84, 94
BtgZI GCGATG 1 cut(s) 443
BtsCI GGATG 1 cut(s) 556
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 2 cut(s) 344, 590
Cac8I GCNNGC 5 cut(s) 122, 186, 426, 469, 535
CfoI GCGC 2 cut(s) 153, 215
Cfr10I RCCGGY 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 493
CseI GACGC 1 cut(s) 43
Csp6I GTAC 3 cut(s) 166, 204, 345
CviAII CATG 1 cut(s) 36
CviJI RGCY 7 cut(s) 43, 84, 94, 184, 238, 373, 464
CviKI_1 RGCY 7 cut(s) 43, 84, 94, 184, 238, 373, 464
CviQI GTAC 3 cut(s) 166, 204, 345
DdeI CTNAG 2 cut(s) 255, 333
DpnI GATC 3 cut(s) 140, 315, 386
DpnII GATC 3 cut(s) 138, 313, 384
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 493
Eco52I CGGCCG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 1 cut(s) 39
FaiI YATR 3 cut(s) 37, 234, 525
FaqI GGGAC 2 cut(s) 211, 506
FatI CATG 1 cut(s) 35
FauI CCCGC 2 cut(s) 193, 481
Fnu4HI GCNGC 4 cut(s) 95, 246, 371, 465
FokI GGATG 1 cut(s) 563
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 4 cut(s) 95, 246, 371, 465
GlaI GCGC 2 cut(s) 152, 214
GluI GCNGC 4 cut(s) 95, 246, 371, 465
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 2 cut(s) 84, 94
HapII CCGG 2 cut(s) 85, 91
HgaI GACGC 1 cut(s) 43
HhaI GCGC 2 cut(s) 153, 215
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 1 cut(s) 39
Hin6I GCGC 2 cut(s) 151, 213
HinP1I GCGC 2 cut(s) 151, 213
HinfI GANTC 2 cut(s) 329, 415
HpaII CCGG 2 cut(s) 85, 91
HphI GGTGA 2 cut(s) 572, 581
Hpy188I TCNGA 4 cut(s) 80, 103, 308, 334
Hpy188III TCNNGA 1 cut(s) 377
HpyAV CCTTC 2 cut(s) 551, 566
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 5 cut(s) 129, 395, 467, 527, 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 251, 434, 464
HpyF3I CTNAG 2 cut(s) 255, 333
HpySE526I ACGT 1 cut(s) 199
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 1 cut(s) 39
HspAI GCGC 2 cut(s) 151, 213
Kzo9I GATC 3 cut(s) 138, 313, 384
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 7 cut(s) 98, 104, 134, 188, 423, 509, 578
Lsp1109I GCAGC 3 cut(s) 232, 382, 451
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 2 cut(s) 155, 510
MalI GATC 3 cut(s) 140, 315, 386
MboI GATC 3 cut(s) 138, 313, 384
MboII GAAGA 4 cut(s) 376, 530, 590, 593
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 3 cut(s) 29, 300, 601
MlyI GAGTC 2 cut(s) 338, 424
MmeI TCCRAC 3 cut(s) 58, 81, 286
MnlI CCTC 8 cut(s) 59, 67, 74, 373, 405, 523, 541, 580
MseI TTAA 1 cut(s) 604
MspA1I CMGCKG 2 cut(s) 97, 124
MspI CCGG 2 cut(s) 85, 91
Mva1269I GAATGC 1 cut(s) 439
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 4 cut(s) 126, 251, 434, 464
NdeII GATC 3 cut(s) 138, 313, 384
NlaIII CATG 1 cut(s) 39
NlaIV GGNNCC 4 cut(s) 20, 89, 183, 494
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 510
PcsI WCGNNNNNNNCGW 2 cut(s) 217, 455
PctI GAATGC 1 cut(s) 439
PkrI GCNGC 4 cut(s) 96, 247, 372, 466
PleI GAGTC 2 cut(s) 337, 423
PpsI GAGTC 2 cut(s) 337, 423
PpuMI RGGWCCY 1 cut(s) 493
Psp1406I AACGTT 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 493
PspN4I GGNNCC 4 cut(s) 20, 89, 183, 494
PspPI GGNCC 1 cut(s) 493
PspPPI RGGWCCY 1 cut(s) 493
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 3 cut(s) 167, 205, 346
RsaNI GTAC 3 cut(s) 166, 204, 345
SaqAI TTAA 1 cut(s) 604
SatI GCNGC 4 cut(s) 95, 246, 371, 465
Sau3AI GATC 3 cut(s) 138, 313, 384
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 2 cut(s) 338, 424
SduI GDGCHC 1 cut(s) 186
SetI ASST 8 cut(s) 51, 78, 202, 240, 375, 466, 498, 562
SinI GGWCC 1 cut(s) 493
Sse9I AATT 3 cut(s) 29, 300, 601
SsiI CCGC 6 cut(s) 12, 95, 124, 186, 320, 488
TaiI ACGT 1 cut(s) 202
TaqI TCGA 3 cut(s) 141, 294, 516
TasI AATT 3 cut(s) 29, 300, 601
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 97
Tru1I TTAA 1 cut(s) 604
Tru9I TTAA 1 cut(s) 604
TscAI CASTG 2 cut(s) 344, 597
TseFI GTSAC 1 cut(s) 510
TseI GCWGC 3 cut(s) 245, 370, 464
Tsp45I GTSAC 1 cut(s) 510
TspDTI ATGAA 2 cut(s) 20, 446
TspGWI ACGGA 2 cut(s) 194, 209
TspRI CASTG 2 cut(s) 344, 597
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.