MD17G1063300.v1.1

protein C9orf85 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006795
Physical Location & Seq
Forward (+)
5175717 .. 5176277
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1063300.v1.1.491

Sequence Viewer

Length: 456 bp
ATGATGAACGGCGGTCGGCACCAAAACAAATTCGCATGGAAGCCAAACCTCGGCGTCAAAATCAACGAAACCGAGGTCGGAGGTCGGTTCCGGCCGCTGTCGGAGATAACGGGGGTCTGCCAGCGCTGCAAGGAACAGATCGAGTGGAAGCGCCGTTACGGAAAGTACAAGACCCTGACGGAGCCCGCCAAGTGCCAACGTTGTACCAAACGCGCCGTCCGTCAGTCCCACCATAAGCTCTGTGCTGCTTGTGCCAAGGAACAACGTGTTTGTGCGAAGTGTTGCTGTCGTGTCGAGCAAATTGTCGGAAAAGACCTTGCGGAAGTGGAGTCTGAGCAGAAGCAGCTTCAAGAGAAAGAGATAGGAGGAGTCTATTGCGTGTGGCATGTTCTCGAATTTGAACACATTTGTTTGTTCTTGTATTGGATTGTGAATCAAAAGTTGCATAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2039

Protein Analysis

152

Amino Acids

17.67

Weight (kDa)

9.19

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2039 PF10217 6 - 96 2.8e-27 Uncharacterized conserved protein (DUF2039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AccII CGCG 1 cut(s) 213
AciI CCGC 4 cut(s) 12, 95, 186, 320
AclI AACGTT 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 2 cut(s) 29, 395
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 167, 205
AfeI AGCGCT 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 2 cut(s) 350, 401
AluBI AGCT 2 cut(s) 238, 346
AluI AGCT 2 cut(s) 238, 346
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 3 cut(s) 126, 245, 343
ApoI RAATTY 2 cut(s) 29, 395
ArsI GACNNNNNNTTYG 4 cut(s) 60, 92, 201, 233
AspLEI GCGC 3 cut(s) 126, 153, 215
BanI GGYRCC 1 cut(s) 18
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbvI GCAGC 3 cut(s) 113, 232, 355
BceAI ACGGC 3 cut(s) 25, 138, 200
BfoI RGCGCY 2 cut(s) 127, 154
BisI GCNGC 4 cut(s) 95, 127, 246, 344
BlsI GCNGC 4 cut(s) 96, 128, 247, 345
BmiI GGNNCC 3 cut(s) 20, 89, 183
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 49, 72, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 50
BseDI CCNNGG 3 cut(s) 49, 72, 255
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 381
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 3 cut(s) 113, 232, 355
Bsh1236I CGCG 1 cut(s) 213
Bsh1285I CGRYCG 2 cut(s) 16, 95
BshFI GGCC 1 cut(s) 94
BshNI GGYRCC 1 cut(s) 18
BsiEI CGRYCG 2 cut(s) 16, 95
BsiSI CCGG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 1 cut(s) 138
BspACI CCGC 4 cut(s) 12, 95, 186, 320
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 3 cut(s) 20, 89, 183
BspT107I GGYRCC 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 49, 72, 255
BssMI GATC 1 cut(s) 138
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 255
BstACI GRCGYC 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 122, 186
BstDEI CTNAG 1 cut(s) 333
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 2 cut(s) 127, 154
BstHHI GCGC 3 cut(s) 126, 153, 215
BstKTI GATC 1 cut(s) 141
BstMBI GATC 1 cut(s) 138
BstMCI CGRYCG 2 cut(s) 16, 95
BstMWI GCNNNNNNNGC 3 cut(s) 126, 251, 343
BstNSI RCATGY 1 cut(s) 389
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 3 cut(s) 113, 232, 355
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 122, 186
CfoI GCGC 3 cut(s) 126, 153, 215
CseI GACGC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 166, 204
CviAII CATG 2 cut(s) 36, 386
CviJI RGCY 5 cut(s) 43, 94, 184, 238, 346
CviKI_1 RGCY 5 cut(s) 43, 94, 184, 238, 346
CviQI GTAC 2 cut(s) 166, 204
DdeI CTNAG 1 cut(s) 333
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 186
Eco47III AGCGCT 1 cut(s) 125
Eco52I CGGCCG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 2 cut(s) 39, 389
FaiI YATR 4 cut(s) 37, 234, 387, 447
FaqI GGGAC 1 cut(s) 211
FatI CATG 2 cut(s) 35, 385
FauI CCCGC 1 cut(s) 193
Fnu4HI GCNGC 4 cut(s) 95, 127, 246, 344
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 4 cut(s) 95, 127, 246, 344
GlaI GCGC 3 cut(s) 125, 152, 214
GluI GCNGC 4 cut(s) 95, 127, 246, 344
HaeII RGCGCY 2 cut(s) 127, 154
HaeIII GGCC 1 cut(s) 94
HapII CCGG 1 cut(s) 91
HgaI GACGC 1 cut(s) 43
HhaI GCGC 3 cut(s) 126, 153, 215
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 39, 389
Hin6I GCGC 3 cut(s) 124, 151, 213
HinP1I GCGC 3 cut(s) 124, 151, 213
HinfI GANTC 3 cut(s) 329, 369, 433
HpaII CCGG 1 cut(s) 91
Hpy188I TCNGA 4 cut(s) 80, 103, 308, 334
Hpy188III TCNNGA 2 cut(s) 350, 392
HpyCH4IV ACGT 2 cut(s) 199, 265
HpyCH4V TGCA 2 cut(s) 129, 445
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 251, 343
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 2 cut(s) 199, 265
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 39, 389
HspAI GCGC 3 cut(s) 124, 151, 213
Kzo9I GATC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 3 cut(s) 104, 134, 188
Lsp1109I GCAGC 3 cut(s) 113, 232, 355
MaeII ACGT 2 cut(s) 199, 265
MaeIII GTNAC 1 cut(s) 155
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 4 cut(s) 29, 300, 395, 448
MlyI GAGTC 2 cut(s) 338, 378
MmeI TCCRAC 3 cut(s) 58, 81, 286
MnlI CCTC 4 cut(s) 59, 67, 74, 359
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 1 cut(s) 91
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 3 cut(s) 126, 251, 343
NdeII GATC 1 cut(s) 138
NlaIII CATG 2 cut(s) 39, 389
NlaIV GGNNCC 3 cut(s) 20, 89, 183
NmeAIII GCCGAG 1 cut(s) 30
NspI RCATGY 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PfeI GAWTC 1 cut(s) 433
PkrI GCNGC 4 cut(s) 96, 128, 247, 345
PleI GAGTC 2 cut(s) 337, 377
PpsI GAGTC 2 cut(s) 337, 377
Psp1406I AACGTT 1 cut(s) 199
PspN4I GGNNCC 3 cut(s) 20, 89, 183
RsaI GTAC 2 cut(s) 167, 205
RsaNI GTAC 2 cut(s) 166, 204
SatI GCNGC 4 cut(s) 95, 127, 246, 344
Sau3AI GATC 1 cut(s) 138
SchI GAGTC 2 cut(s) 338, 378
SduI GDGCHC 1 cut(s) 186
SetI ASST 8 cut(s) 51, 78, 85, 202, 240, 268, 318, 348
Sse9I AATT 4 cut(s) 29, 300, 395, 448
SsiI CCGC 4 cut(s) 12, 95, 186, 320
StyI CCWWGG 1 cut(s) 255
TaiI ACGT 2 cut(s) 202, 268
TaqI TCGA 3 cut(s) 141, 294, 393
TasI AATT 4 cut(s) 29, 300, 395, 448
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 1 cut(s) 433
TseI GCWGC 3 cut(s) 126, 245, 343
TspDTI ATGAA 1 cut(s) 20
TspGWI ACGGA 3 cut(s) 174, 194, 209
XapI RAATTY 2 cut(s) 29, 395
XceI RCATGY 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.