Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001943

Physical Location & Seq

Genomic Coordinates Reverse (-)

12492835 .. 12500232

Gene Structure 7398 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1156900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3093 bp

ATGGAAAGCCATCAGCTTTCATTCCCAAAGTTGCTTCTTGCATGTCTCATCTGTTCCTCTACATTTTTGTTCTTTGCAACTTTTGGTCAGTCAGCCACCACCGCCAAGCTTCACACTCAAGAAGTGAATGTATTGAAAGAGATAGGGAAAAAGATGGGTAAGAAAGACTGGGATTTTGGAAAAGATCCATGTACGGAAGAAGGGAATTGGACGGTCCCGATTAGTACGGGGAAGAAGGCCTTTGACAGTGCTGTCATATGTGATTGCACCTTCAACCACAATTCCTCTTGCCACGTCATCAGAATATTTCTAAAGTCCCAGAATCTTTCAGGGACCGTGCCACCAGAGTTTTCGAAGCTTCGTTACCTAGAAGAATTAGACTTGAGTCGAAATGTCCTCAATGGTACTATACCTTCCCAATGGGCTACCATGCGCTTGCACACGCTGTCTTTTATGGGAAACAGATTGTCAGGACCATTTCCAAAAGTTCTTACCAATATCACCACCCTCAGAAACTTGAGCATTGAAGGAAACCATTTCTCAGGACCCATTCCGTGGGAAATTGGAAAGTTGATCAAACTAGAAAAGCTCATTCTGTCTTCAAACGCCTTCACCGGAAAATTGCCATCGACACTAGGAAAGCTGACGAACTTGTCTGATATGAGGATAAATGACAATAATTTCTCTGGAAAGATACCTGACTTCATTGGAAATTGGACGCGGGTTTCAAAACTGCTTATCGAGGGTAGCTCTCTTGAGGGGCCTATACCTTCTACCATATCTGCCTTAACAAGTTTAACTGATCTGAGGATAACCGACTTAAAAGGAAACAGGTCTCCTTTCCCGCCATTGAGTAATTTAGAATCCTTGAAAACATTGATACTGAGGAATTGCTTGATACACGGAGAAATCCCAGATTACATTGGGGATATGGAAAACTTGAAAAACTTAGACCTCAGCTTCAATGAGTTGACGGGTGAAATACCTGCATCTTTTGTCCGACTGGAAAAAATTGACATTATGTATTTGACAGGAAACCAGCTCACTGGAACCATACCGGGATGGGTCCCGTCAAGTAACAATAATGTGGATATTTCTTACAACAACTTCACTTGGGAAGGTTCAAGTCCTAATGAATGCTCTAGGGGAAGTGTCAACTTAGTGGAGAGCTACTCCTCATCAGCTGATAAATTAAAAATACAACCGTGCATGAAAAGAAATTTTCCTTGTCCTGCTTCGAAAGATCAGCGTAAACATTCACTGTATATTAACTGTGGCGGAAAGGAAGTAATGATCGGTGACAAGAAGTATGAAGCTGATAGAGAACCAAGGGGTGCATCGATGTATTACATGGGTGAGAACTGGGCTTTCAGCAGCACAGGAAACTTCATGGACAATGATGCTGATTCAGATACCTACATTGACACCAATACATCTGCACTTTCAAAAAATGTCTCTGCGCTTGATTCAGAACTGTACATGACAGCGCGCGCATCTCCCATCTTGCTCACATACTATGGTCTCTGTCTCATAAATGGAAACTACTCCGTGCAACTCCACTTTGCTGAGATTGTTTTTACTAATGACAGAACATTTTACAGCCTTGGCAAGCGTGTATTTGATGTCTACATCCAGGATAAGCTGGTCCTGAAAGATTTCGATATAGCAAGTGAAGCTGGGGATGCTGGCAAGCCGATTGTTAAAGAGTTCACTTCGGTTGTTTCGAGTCATACATTAAAGATCCATTTTTACTGGGCTGGAAAAGGGACGACGGGCATCCCAGACAGAGGATTTTATGGTCCTCTCATATCGGCTATATCAGTAGATCCTAATTTTAAACCTCCTTCGGTTGATGGCCACAAGAATCGTCTCGTTAAAGTAGTTGGGGGAGTGGCTGGAGCACTAGTACTTCTTCTGCTTCTGGTCTTAGGCATCATGAGGAAAAAGGGCTGTTTGGGAGGAAAGATCGCTGCAGATAAAGAATTCAAAGATTTAGATCTTCAAACAGGACTATATACTTTAAGACAGATAAAGGCTGCAACAAAAAACTTTGACGCAGCTAACAAACTTGGGGAGGGCGGCTTTGGTGCGGTCTACAAGGGTGAATTATCAGACGGCACAGTAATTGCGGTGAAGCAGCTGTCCTCAAAATCAAAGCAAGGAAACCGTGAATTTGTGAATGAAATCGGCATGATTTCTGCCCTGCAACATCCAAACCTTGTGAAGCTTTATGGGTGCTGTGTTGAAGGGAACCAGATGCTGTTAATTTACGAGTACATGGAAAATAACTGTGTGTCACGTGCTCTTTTCGGAAGCGACCCGACTTGCAGACTGAAACTCGACTGGCCTACAAGGAAGAAGATTTGCATAGGTATTGCTAGAGGCTTGGCCTATCTCCACGAAGAATCAAGACTAAGAATCGTGCATCGTGATATCAAGACAAGCAATGTGTTGCTTGATAAGGACTTCAATGCTAAAATTTCAGATTTTGGCTTGGCAAAGCTTAATGAAGACGACAAAACCCACATCAGCACGCGGGTTGCTGGAACCATTGGCTATATGGCTCCTGAGTATGCAATGCGTGGTTACTTGACTGACAAGGCAGATGTTTATAGCTTTGGAGTTGTTGCACTTGAAATCGTTAGTGGAAAGAGCAACACAAACTACAGGCCAAAGGAGGAATTCGTCTACCTTCTTGACTGGGCCTATGTGCTGCAAGAGAGAGGGAGTCTATTGGAGTTGGTTGATCCAGCCTTGGGTTCAGAGTACTCATCAGAAGAGACCATGGTGGTGCTAAATGTTGCTCTCTTGTGCACCAATGCATCTCCCACCCTTAGGCCTACAATGACCCAAGTAGTGAGCATGCTTGAAGGACGAACGGCGGTTCAAGACCTGCTTTCGGATCCCGGGTTTTCAGCCATCAATTCCAAGGTCAGGGCCATCAGAAATCACTTCTGGCAACATCCAAGTTGTACTCAGAGCATGTCAACCAACGGTCCGAAAACTGATACTTCCGGTTCGTACATTGAAACAGAAGAGAATGCTGATCTCCTGAGAGTTGGATCATTGACTTCGGTTAAATCTGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1031

Amino Acids

113.82

Weight (kDa)

7.0

Isoelectric Point (pI)

27.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	164 - 272	1.4e-08	Leucine-rich repeat region
LRR_8	PF13855	289 - 348	2.3e-08	Leucine rich repeat
Malectin	PF11721	421 - 606	6.7e-40	Malectin domain
Pkinase	PF00069	686 - 954	9.5e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	687 - 956	5.4e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07650 AT1G07650 AT1G07650 AT1G07650
fragaria_vesca	FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550
malus_domestica	MD09G1156900.v1.1
prunus_persica	Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1
pyrus_communis	pycom09g07560 pycom11g23700
rosa_chinensis	RchiOBHm_Chr2g0149971 RchiOBHm_Chr2g0150431
rosa_laevigata	RLG00000000535 RLG00000001460 RLG00000011700 RLG00000016818 RLG00000020498 RLG00000030032 RLG00000034736
rosa_multiflora	Rmu_co8438829.1_g000001 Rmu_sc0020041.1_g000001 Rmu_ssc0000480.1_g000017
rosa_roxburghii	Rroxscaffold_2G00097110
rosa_rugosa	Rorug02G0421300
rosa_samantha	Rh2DG501600 Rh4AG126900
rosa_wichuraiana	Rw2G039240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	251, 652
Acc36I	ACCTGC	2 cut(s)	994, 2901
AccB7I	CCANNNNNTGG	1 cut(s)	555
AccI	GTMKAC	3 cut(s)	1626, 2094, 2688
AccII	CGCG	4 cut(s)	721, 1489, 1491, 2536
AciI	CCGC	9 cut(s)	102, 721, 845, 1278, 2079, 2090, 2129, 2536, 2882
AclWI	GGATC	7 cut(s)	179, 1735, 1820, 2741, 2897, 2910, 3070
AcoI	YGGCCR	1 cut(s)	1855
AcsI	RAATTY	5 cut(s)	1219, 1982, 2171, 2478, 2681
AcvI	CACGTG	1 cut(s)	2300
AfaI	GTAC	9 cut(s)	193, 226, 406, 1478, 1908, 2276, 2768, 2974, 3023
AfiI	CCNNNNNNNGG	6 cut(s)	555, 1787, 2756, 2835, 2899, 2934
AhlI	ACTAGT	1 cut(s)	1903
AjiI	CACGTC	1 cut(s)	295
AjnI	CCWGG	1 cut(s)	1632
Alw21I	GWGCWC	3 cut(s)	1903, 2305, 2816
Alw26I	GTCTC	7 cut(s)	50, 840, 1459, 1526, 1532, 1874, 2774
Alw44I	GTGCAC	1 cut(s)	2812
AlwI	GGATC	7 cut(s)	179, 1735, 1820, 2741, 2897, 2910, 3070
AlwNI	CAGNNNCTG	1 cut(s)	2260
Ama87I	CYCGRG	1 cut(s)	2906
AoxI	GGCC	9 cut(s)	237, 761, 1855, 2345, 2388, 2669, 2703, 2837, 2937
ApaLI	GTGCAC	1 cut(s)	2812
ApeKI	GCWGC	6 cut(s)	1374, 1970, 2036, 2057, 2137, 2713
ApoI	RAATTY	5 cut(s)	1219, 1982, 2171, 2478, 2681
ArsI	GACNNNNNNTTYG	2 cut(s)	158, 190
Asp700I	GAANNNNTTC	1 cut(s)	1655
AspLEI	GCGC	5 cut(s)	435, 1462, 1489, 1491, 1493
AsuC2I	CCSGG	3 cut(s)	1059, 2907, 2908
AsuHPI	GGTGA	7 cut(s)	493, 604, 989, 1310, 1367, 2114, 2143
AsuII	TTCGAA	2 cut(s)	353, 1238
AvaI	CYCGRG	1 cut(s)	2906
AvaII	GGWCC	8 cut(s)	214, 333, 473, 545, 1066, 1645, 1799, 2996
AxyI	CCTNAGG	1 cut(s)	2834
BaeGI	GKGCMC	1 cut(s)	2816
BalI	TGGCCA	1 cut(s)	1857
BamHI	GGATCC	1 cut(s)	2902
BarI	GAAGNNNNNNTAC	4 cut(s)	347, 379, 397, 429
BbrPI	CACGTG	1 cut(s)	2300
BbsI	GAAGAC	2 cut(s)	591, 2517
Bbv12I	GWGCWC	3 cut(s)	1903, 2305, 2816
BbvCI	CCTCAGC	1 cut(s)	956
BbvI	GCAGC	6 cut(s)	1386, 1957, 2023, 2069, 2149, 2700
BccI	CCATC	8 cut(s)	18, 148, 634, 1056, 1508, 1847, 2927, 2948
BceAI	ACGGC	2 cut(s)	2131, 2895
BciT130I	CCWGG	1 cut(s)	1634
BclI	TGATCA	1 cut(s)	573
BcnI	CCSGG	3 cut(s)	1059, 2907, 2908
BcoDI	GTCTC	7 cut(s)	50, 840, 1459, 1526, 1532, 1874, 2774
BcuI	ACTAGT	1 cut(s)	1903
BfaI	CTAG	6 cut(s)	368, 581, 635, 1143, 1904, 2379
BfmI	CTRYAG	2 cut(s)	1971, 2665
BfuAI	ACCTGC	2 cut(s)	994, 2901
BglII	AGATCT	1 cut(s)	1996
BisI	GCNGC	7 cut(s)	1375, 1971, 2037, 2058, 2080, 2138, 2714
BlsI	GCNGC	7 cut(s)	1376, 1972, 2038, 2059, 2081, 2139, 2715
BmcAI	AGTACT	2 cut(s)	1908, 2768
Bme1390I	CCNGG	4 cut(s)	1059, 1634, 2907, 2908
Bme18I	GGWCC	8 cut(s)	214, 333, 473, 545, 1066, 1645, 1799, 2996
BmeT110I	CYCGRG	1 cut(s)	2906
BmgBI	CACGTC	1 cut(s)	295
BmrFI	CCNGG	4 cut(s)	1059, 1634, 2907, 2908
BmrI	ACTGGG	4 cut(s)	178, 1372, 1762, 2710
BmuI	ACTGGG	4 cut(s)	178, 1372, 1762, 2710
BoxI	GACNNNNGTC	1 cut(s)	384
BpiI	GAAGAC	2 cut(s)	591, 2517
BplI	GAGNNNNNCTC	4 cut(s)	734, 766, 1157, 1189
BpmI	CTGGAG	1 cut(s)	1917
Bpu10I	CCTNAGC	1 cut(s)	956
Bpu14I	TTCGAA	2 cut(s)	353, 1238
BpuEI	CTTGAG	4 cut(s)	102, 403, 538, 776
BpuMI	CCSGG	3 cut(s)	1059, 2907, 2908
Bsa29I	ATCGAT	1 cut(s)	1340
BsaAI	YACGTR	1 cut(s)	2300
BsaBI	GATNNNNATC	1 cut(s)	1995
BsaI	GGTCTC	3 cut(s)	840, 1526, 2774
BsaJI	CCNNGG	7 cut(s)	554, 1328, 1603, 2754, 2784, 2906, 2928
BsaWI	WCCGGW	2 cut(s)	614, 3014
BsaXI	ACNNNNNCTCC	2 cut(s)	1890, 1920
Bsc4I	CCNNNNNNNGG	6 cut(s)	555, 1787, 2756, 2835, 2899, 2934
Bse1I	ACTGG	7 cut(s)	173, 1008, 1051, 1367, 1757, 2348, 2705
Bse21I	CCTNAGG	1 cut(s)	2834
Bse3DI	GCAATG	2 cut(s)	2452, 2583
Bse8I	GATNNNNATC	1 cut(s)	1995
BseBI	CCWGG	1 cut(s)	1634
BseCI	ATCGAT	1 cut(s)	1340
BseDI	CCNNGG	7 cut(s)	554, 1328, 1603, 2754, 2784, 2906, 2928
BseGI	GGATG	6 cut(s)	1067, 1629, 1687, 1776, 2209, 2962
BseJI	GATNNNNATC	1 cut(s)	1995
BseLI	CCNNNNNNNGG	6 cut(s)	555, 1787, 2756, 2835, 2899, 2934
BseMI	GCAATG	2 cut(s)	2452, 2583
BseMII	CTCAG	9 cut(s)	523, 555, 797, 875, 970, 1557, 2559, 2990, 3044
BseNI	ACTGG	7 cut(s)	173, 1008, 1051, 1367, 1757, 2348, 2705
BsePI	GCGCGC	2 cut(s)	1487, 1489
BseRI	GAGGAG	1 cut(s)	1165
BseSI	GKGCMC	1 cut(s)	2816
BseXI	GCAGC	6 cut(s)	1386, 1957, 2023, 2069, 2149, 2700
BseYI	CCCAGC	1 cut(s)	1676
BsgI	GTGCAG	1 cut(s)	1422
Bsh1236I	CGCG	4 cut(s)	721, 1489, 1491, 2536
BshFI	GGCC	9 cut(s)	239, 763, 1857, 2347, 2390, 2671, 2705, 2839, 2939
BshVI	ATCGAT	1 cut(s)	1340
BsiHKAI	GWGCWC	3 cut(s)	1903, 2305, 2816
BsiHKCI	CYCGRG	1 cut(s)	2906
BsiSI	CCGG	4 cut(s)	615, 1058, 2907, 3015
BslFI	GGGAC	5 cut(s)	200, 301, 346, 1052, 1780
BslI	CCNNNNNNNGG	6 cut(s)	555, 1787, 2756, 2835, 2899, 2934
BsmAI	GTCTC	7 cut(s)	50, 840, 1459, 1526, 1532, 1874, 2774
BsmBI	CGTCTC	1 cut(s)	1874
BsmFI	GGGAC	5 cut(s)	200, 301, 346, 1052, 1780
BsmI	GAATGC	2 cut(s)	1142, 3046
BsnI	GGCC	9 cut(s)	239, 763, 1857, 2347, 2390, 2671, 2705, 2839, 2939
Bso31I	GGTCTC	3 cut(s)	840, 1526, 2774
BsoBI	CYCGRG	1 cut(s)	2906
Bsp119I	TTCGAA	2 cut(s)	353, 1238
Bsp1286I	GDGCHC	3 cut(s)	1903, 2305, 2816
Bsp1407I	TGTACA	1 cut(s)	1476
Bsp19I	CCATGG	1 cut(s)	2784
BspACI	CCGC	9 cut(s)	102, 721, 845, 1278, 2079, 2090, 2129, 2536, 2882
BspANI	GGCC	9 cut(s)	239, 763, 1857, 2347, 2390, 2671, 2705, 2839, 2939
BspCNI	CTCAG	9 cut(s)	522, 554, 798, 876, 969, 1558, 2560, 2989, 3045
BspDI	ATCGAT	1 cut(s)	1340
BspFNI	CGCG	4 cut(s)	721, 1489, 1491, 2536
BspHI	TCATGA	1 cut(s)	1935
BspMAI	CTGCAG	1 cut(s)	1975
BspMI	ACCTGC	2 cut(s)	994, 2901
BspPI	GGATC	7 cut(s)	179, 1735, 1820, 2741, 2897, 2910, 3070
BspT104I	TTCGAA	2 cut(s)	353, 1238
BspTNI	GGTCTC	3 cut(s)	840, 1526, 2774
BsrDI	GCAATG	2 cut(s)	2452, 2583
BsrGI	TGTACA	1 cut(s)	1476
BsrI	ACTGG	7 cut(s)	173, 1008, 1051, 1367, 1757, 2348, 2705
BssECI	CCNNGG	7 cut(s)	554, 1328, 1603, 2754, 2784, 2906, 2928
BssHII	GCGCGC	2 cut(s)	1487, 1489
BssT1I	CCWWGG	5 cut(s)	1328, 1603, 2754, 2784, 2928
Bst2UI	CCWGG	1 cut(s)	1634
Bst6I	CTCTTC	2 cut(s)	2772, 3030
BstAUI	TGTACA	1 cut(s)	1476
BstBAI	YACGTR	1 cut(s)	2300
BstBI	TTCGAA	2 cut(s)	353, 1238
BstC8I	GCNNGC	8 cut(s)	437, 1489, 1491, 1610, 1687, 1691, 2534, 2864
BstDSI	CCRYGG	2 cut(s)	554, 2784
BstF5I	GGATG	6 cut(s)	1067, 1629, 1687, 1776, 2209, 2962
BstFNI	CGCG	4 cut(s)	721, 1489, 1491, 2536
BstHHI	GCGC	5 cut(s)	435, 1462, 1489, 1491, 1493
BstMAI	GTCTC	7 cut(s)	50, 840, 1459, 1526, 1532, 1874, 2774
BstMWI	GCNNNNNNNGC	3 cut(s)	101, 1673, 1682
BstNI	CCWGG	1 cut(s)	1634
BstNSI	RCATGY	3 cut(s)	45, 2866, 2986
BstPAI	GACNNNNGTC	1 cut(s)	384
BstSCI	CCNGG	4 cut(s)	1057, 1632, 2905, 2906
BstSFI	CTRYAG	2 cut(s)	1971, 2665
BstSLI	GKGCMC	1 cut(s)	2816
BstUI	CGCG	4 cut(s)	721, 1489, 1491, 2536
BstV1I	GCAGC	6 cut(s)	1386, 1957, 2023, 2069, 2149, 2700
BstV2I	GAAGAC	2 cut(s)	591, 2517
BstX2I	RGATCY	5 cut(s)	184, 1740, 1825, 1996, 2902
BstXI	CCANNNNNNTGG	1 cut(s)	1046
BstYI	RGATCY	5 cut(s)	184, 1740, 1825, 1996, 2902
Bsu15I	ATCGAT	1 cut(s)	1340
Bsu36I	CCTNAGG	1 cut(s)	2834
BsuRI	GGCC	9 cut(s)	239, 763, 1857, 2347, 2390, 2671, 2705, 2839, 2939
BsuTUI	ATCGAT	1 cut(s)	1340
BtgI	CCRYGG	2 cut(s)	554, 2784
BtrI	CACGTC	1 cut(s)	295
BtsCI	GGATG	6 cut(s)	1067, 1629, 1687, 1776, 2209, 2962
BtsIMutI	CAGTG	3 cut(s)	253, 1044, 1259
BveI	ACCTGC	2 cut(s)	994, 2901
Cac8I	GCNNGC	8 cut(s)	437, 1489, 1491, 1610, 1687, 1691, 2534, 2864
CaiI	CAGNNNCTG	1 cut(s)	2260
CciI	TCATGA	1 cut(s)	1935
CfoI	GCGC	5 cut(s)	435, 1462, 1489, 1491, 1493
Cfr9I	CCCGGG	1 cut(s)	2906
ClaI	ATCGAT	1 cut(s)	1340
CpoI	CGGWCCG	1 cut(s)	2996
CseI	GACGC	2 cut(s)	727, 2063
Csp6I	GTAC	9 cut(s)	192, 225, 405, 1477, 1907, 2275, 2767, 2973, 3022
CspI	CGGWCCG	1 cut(s)	2996
CviQI	GTAC	9 cut(s)	192, 225, 405, 1477, 1907, 2275, 2767, 2973, 3022
DraI	TTTAAA	1 cut(s)	1837
DrdI	GACNNNNNNGTC	2 cut(s)	251, 652
DseDI	GACNNNNNNGTC	2 cut(s)	251, 652
EaeI	YGGCCR	1 cut(s)	1855
Eam1104I	CTCTTC	2 cut(s)	2772, 3030
EarI	CTCTTC	2 cut(s)	2772, 3030
EciI	GGCGGA	1 cut(s)	1293
Eco130I	CCWWGG	5 cut(s)	1328, 1603, 2754, 2784, 2928
Eco147I	AGGCCT	2 cut(s)	239, 2839
Eco31I	GGTCTC	3 cut(s)	840, 1526, 2774
Eco32I	GATATC	1 cut(s)	2434
Eco47I	GGWCC	8 cut(s)	214, 333, 473, 545, 1066, 1645, 1799, 2996
Eco72I	CACGTG	1 cut(s)	2300
Eco81I	CCTNAGG	1 cut(s)	2834
Eco88I	CYCGRG	1 cut(s)	2906
EcoO109I	RGGNCCY	3 cut(s)	545, 761, 1066
EcoRI	GAATTC	2 cut(s)	1982, 2681
EcoRII	CCWGG	1 cut(s)	1632
EcoRV	GATATC	1 cut(s)	2434
EcoT14I	CCWWGG	5 cut(s)	1328, 1603, 2754, 2784, 2928
EcoT22I	ATGCAT	1 cut(s)	2824
ErhI	CCWWGG	5 cut(s)	1328, 1603, 2754, 2784, 2928
Esp3I	CGTCTC	1 cut(s)	1874
FalI	AAGNNNNNCTT	4 cut(s)	224, 256, 2880, 2912
FaqI	GGGAC	5 cut(s)	200, 301, 346, 1052, 1780
FauI	CCCGC	3 cut(s)	714, 852, 2529
FauNDI	CATATG	1 cut(s)	257
FbaI	TGATCA	1 cut(s)	573
FblI	GTMKAC	3 cut(s)	1626, 2094, 2688
Fnu4HI	GCNGC	7 cut(s)	1375, 1971, 2037, 2058, 2080, 2138, 2714
FokI	GGATG	6 cut(s)	1074, 1616, 1694, 1763, 2196, 2949
Fsp4HI	GCNGC	7 cut(s)	1375, 1971, 2037, 2058, 2080, 2138, 2714
FspBI	CTAG	6 cut(s)	368, 581, 635, 1143, 1904, 2379
GlaI	GCGC	5 cut(s)	434, 1461, 1488, 1490, 1492
GluI	GCNGC	7 cut(s)	1375, 1971, 2037, 2058, 2080, 2138, 2714
GsaI	CCCAGC	1 cut(s)	1680
GsuI	CTGGAG	1 cut(s)	1917
HaeIII	GGCC	9 cut(s)	239, 763, 1857, 2347, 2390, 2671, 2705, 2839, 2939
HapII	CCGG	4 cut(s)	615, 1058, 2907, 3015
HgaI	GACGC	2 cut(s)	727, 2063
HhaI	GCGC	5 cut(s)	435, 1462, 1489, 1491, 1493
Hin6I	GCGC	5 cut(s)	433, 1460, 1487, 1489, 1491
HinP1I	GCGC	5 cut(s)	433, 1460, 1487, 1489, 1491
HincII	GTYRAC	3 cut(s)	972, 1156, 2988
HindII	GTYRAC	3 cut(s)	972, 1156, 2988
HindIII	AAGCTT	4 cut(s)	107, 356, 2225, 2501
HpaII	CCGG	4 cut(s)	615, 1058, 2907, 3015
HphI	GGTGA	7 cut(s)	493, 604, 989, 1310, 1367, 2114, 2143
Hpy166II	GTNNAC	9 cut(s)	972, 1156, 1253, 1627, 1710, 2095, 2689, 2814, 2988
Hpy8I	GTNNAC	9 cut(s)	972, 1156, 1253, 1627, 1710, 2095, 2689, 2814, 2988
Hpy99I	CGWCG	1 cut(s)	1774
HpyCH4IV	ACGT	2 cut(s)	294, 2299
HpyF10VI	GCNNNNNNNGC	3 cut(s)	101, 1673, 1682
HpySE526I	ACGT	2 cut(s)	294, 2299
HspAI	GCGC	5 cut(s)	433, 1460, 1487, 1489, 1491
KflI	GGGWCCC	1 cut(s)	1066
Ksp22I	TGATCA	1 cut(s)	573
LmnI	GCTCC	2 cut(s)	1898, 2569
Lsp1109I	GCAGC	6 cut(s)	1386, 1957, 2023, 2069, 2149, 2700
MaeI	CTAG	6 cut(s)	368, 581, 635, 1143, 1904, 2379
MaeII	ACGT	2 cut(s)	294, 2299
MaeIII	GTNAC	5 cut(s)	362, 1076, 1298, 2295, 2585
MflI	RGATCY	5 cut(s)	184, 1740, 1825, 1996, 2902
MhlI	GDGCHC	3 cut(s)	1903, 2305, 2816
MlsI	TGGCCA	1 cut(s)	1857
MluNI	TGGCCA	1 cut(s)	1857
MlyI	GAGTC	3 cut(s)	394, 1735, 2737
MmeI	TCCRAC	2 cut(s)	1024, 3040
Mox20I	TGGCCA	1 cut(s)	1857
Mph1103I	ATGCAT	1 cut(s)	2824
MroXI	GAANNNNTTC	1 cut(s)	1655
MscI	TGGCCA	1 cut(s)	1857
MslI	CAYNNNNRTG	1 cut(s)	2789
Msp20I	TGGCCA	1 cut(s)	1857
MspA1I	CMGCKG	2 cut(s)	1184, 2140
MspI	CCGG	4 cut(s)	615, 1058, 2907, 3015
MspR9I	CCNGG	4 cut(s)	1059, 1634, 2907, 2908
Mva1269I	GAATGC	2 cut(s)	1142, 3046
MvaI	CCWGG	1 cut(s)	1634
MvnI	CGCG	4 cut(s)	721, 1489, 1491, 2536
MwoI	GCNNNNNNNGC	3 cut(s)	101, 1673, 1682
NciI	CCSGG	3 cut(s)	1059, 2907, 2908
NcoI	CCATGG	1 cut(s)	2784
NdeI	CATATG	1 cut(s)	257
NmuCI	GTSAC	2 cut(s)	1298, 2295
NsiI	ATGCAT	1 cut(s)	2824
NspI	RCATGY	3 cut(s)	45, 2866, 2986
NspV	TTCGAA	2 cut(s)	353, 1238
PaeI	GCATGC	1 cut(s)	2866
PagI	TCATGA	1 cut(s)	1935
PauI	GCGCGC	2 cut(s)	1487, 1489
PceI	AGGCCT	2 cut(s)	239, 2839
PcsI	WCGNNNNNNNCGW	1 cut(s)	1721
PctI	GAATGC	2 cut(s)	1142, 3046
PdmI	GAANNNNTTC	1 cut(s)	1655
PfeI	GAWTC	7 cut(s)	322, 863, 1406, 1466, 1864, 2405, 2418
PflMI	CCANNNNNTGG	1 cut(s)	555
PfoI	TCCNGGA	1 cut(s)	1632
PkrI	GCNGC	7 cut(s)	1376, 1972, 2038, 2059, 2081, 2139, 2715
PleI	GAGTC	3 cut(s)	393, 1734, 2736
PmaCI	CACGTG	1 cut(s)	2300
PmlI	CACGTG	1 cut(s)	2300
PpsI	GAGTC	3 cut(s)	393, 1734, 2736
Ppu21I	YACGTR	1 cut(s)	2300
PpuMI	RGGWCCY	2 cut(s)	545, 1066
PshAI	GACNNNNGTC	1 cut(s)	384
Psp5II	RGGWCCY	2 cut(s)	545, 1066
Psp6I	CCWGG	1 cut(s)	1632
PspCI	CACGTG	1 cut(s)	2300
PspFI	CCCAGC	1 cut(s)	1676
PspGI	CCWGG	1 cut(s)	1632
PspPPI	RGGWCCY	2 cut(s)	545, 1066
PstI	CTGCAG	1 cut(s)	1975
PstNI	CAGNNNCTG	1 cut(s)	2260
PsuI	RGATCY	5 cut(s)	184, 1740, 1825, 1996, 2902
PteI	GCGCGC	2 cut(s)	1487, 1489
PvuII	CAGCTG	2 cut(s)	1184, 2140
RsaI	GTAC	9 cut(s)	193, 226, 406, 1478, 1908, 2276, 2768, 2974, 3023
RsaNI	GTAC	9 cut(s)	192, 225, 405, 1477, 1907, 2275, 2767, 2973, 3022
RseI	CAYNNNNRTG	1 cut(s)	2789
Rsr2I	CGGWCCG	1 cut(s)	2996
RsrII	CGGWCCG	1 cut(s)	2996
SatI	GCNGC	7 cut(s)	1375, 1971, 2037, 2058, 2080, 2138, 2714
ScaI	AGTACT	2 cut(s)	1908, 2768
SchI	GAGTC	3 cut(s)	394, 1735, 2737
ScrFI	CCNGG	4 cut(s)	1059, 1634, 2907, 2908
SduI	GDGCHC	3 cut(s)	1903, 2305, 2816
SfcI	CTRYAG	2 cut(s)	1971, 2665
SfuI	TTCGAA	2 cut(s)	353, 1238
SinI	GGWCC	8 cut(s)	214, 333, 473, 545, 1066, 1645, 1799, 2996
SmaI	CCCGGG	1 cut(s)	2908
SmiMI	CAYNNNNRTG	1 cut(s)	2789
SmlI	CTYRAG	4 cut(s)	117, 382, 517, 755
SmoI	CTYRAG	4 cut(s)	117, 382, 517, 755
SpeI	ACTAGT	1 cut(s)	1903
SphI	GCATGC	1 cut(s)	2866
SseBI	AGGCCT	2 cut(s)	239, 2839
SsiI	CCGC	9 cut(s)	102, 721, 845, 1278, 2079, 2090, 2129, 2536, 2882
SspI	AATATT	1 cut(s)	306
SspMI	CTAG	6 cut(s)	368, 581, 635, 1143, 1904, 2379
StuI	AGGCCT	2 cut(s)	239, 2839
StyD4I	CCNGG	4 cut(s)	1057, 1632, 2905, 2906
StyI	CCWWGG	5 cut(s)	1328, 1603, 2754, 2784, 2928
TaiI	ACGT	2 cut(s)	297, 2302
TaqI	TCGA	9 cut(s)	353, 388, 629, 741, 1238, 1340, 1659, 1724, 2340
TatI	WGTACW	5 cut(s)	1476, 1906, 2274, 2766, 2972
TauI	GCSGC	1 cut(s)	2082
TfiI	GAWTC	7 cut(s)	322, 863, 1406, 1466, 1864, 2405, 2418
TscAI	CASTG	3 cut(s)	253, 1051, 1266
TseFI	GTSAC	2 cut(s)	1298, 2295
TseI	GCWGC	6 cut(s)	1374, 1970, 2036, 2057, 2137, 2713
Tsp45I	GTSAC	2 cut(s)	1298, 2295
TspDTI	ATGAA	8 cut(s)	9, 694, 1149, 1226, 1326, 1378, 2196, 2523
TspGWI	ACGGA	4 cut(s)	209, 543, 918, 1537
TspMI	CCCGGG	1 cut(s)	2906
TspRI	CASTG	3 cut(s)	253, 1051, 1266
Van91I	CCANNNNNTGG	1 cut(s)	555
VneI	GTGCAC	1 cut(s)	2812
VpaK11BI	GGWCC	8 cut(s)	214, 333, 473, 545, 1066, 1645, 1799, 2996
XapI	RAATTY	5 cut(s)	1219, 1982, 2171, 2478, 2681
XceI	RCATGY	3 cut(s)	45, 2866, 2986
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2557
XmaI	CCCGGG	1 cut(s)	2906
XmiI	GTMKAC	3 cut(s)	1626, 2094, 2688
XmnI	GAANNNNTTC	1 cut(s)	1655
XspI	CTAG	6 cut(s)	368, 581, 635, 1143, 1904, 2379
ZrmI	AGTACT	2 cut(s)	1908, 2768
Zsp2I	ATGCAT	1 cut(s)	2824

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.