Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001943

Physical Location & Seq

Genomic Coordinates Forward (+)

65643896 .. 65650545

Gene Structure 6650 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020498

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2907 bp

ATGGCCCAGATGGGAACTGACCATTTTTCATTCCCAAGGTTGCTTCTTGCATATCTCATCTTCAGTTTCACTGCATTTTTCTTTCCTGCAAGTTTTGGACAAGCAGCCACAGCTAAGCTTCAGAGCCAGGAAGTGAATGCATTGAAAGAGATAGCAAAGAAGTTGGGGAAGAAAGACTGGGATTTTGGGAAAGATCCTTGCACAGGAGAAGGGAATTGGAGAGTTTTTGTAGATGGGATGAAAAGCTTTGAGAGCTCTGTAACTTGTGATTGCAGCTTCAACAACAATTCCTCTTGCCACGTAGTCAGCATAGCTTTGAAGGCACAGAATTTAACTGGTACACTGCCACCAGAGTTCTCAAAGCTTCATTACCTCAAAGTATTAGATGTGAGTAGAAACCTCTTTACTGGTTCCATACCTTCTCAATGGGCAACCATGCAGTTGGAGGAACTAGGTCTTATGGGGAACAGATTGTCGGGTCCATTTCCAAAAGTCCTCACCAATATTACCACCCTCAGAAACTTGAGCATAGAAGGAAACCTTTTTTCAGGGCCCATTCCGTTGGAAATTGGAAAGTTGGTCAAGTTAGAAAAGCTCATTTTATCATCAAATCTATTCACCGGAGAATTGCCACAAGCACTAGCAGAGTTGATCAATTTGCTTGACATGAGGATAACTGACAACAACTTCTCTGGAAAGATACCTGACTTCATTGGAAACTGGACGCAGATTTCGAAATTGCTAATTCAAGGTTGCCCTTTTGAGGGTCCTATACCTTCTAGTATATCAGCCTTAACACACTTGATTGATCTGAGGATAACCGACTTAAAAGGTAGAGGGTCTTCTTTCCCGCCATTGAGTAAAATGGCTTTCTTGAAAACATTGGTACTGAGGAACTGCTTAATATATGGAGAGATCCCGCATTACATTGGCGATATGACAAAATTGAAAAACCTTGACCTCAGCTATAATGAGTTGACTGGTGCAGTACCAGTATCGTTTGACAAACTGACAAAAGTTGATTTCATATATTTAACAGCAAACAAGCTTACTGGAACTATACCTGGATGGGTCTTGAAACGAATCAAAACAGTGGATTTTTCTTACAACAACTTCACTTGGGAAAACTCAAGCTCACTTCCTACTGAATGTGATAGAGGGAGTGTAAATTTAGTAGAAAGCTATTCCTCATCAGCTGATAATATAAGTAGAGTCGCAACATGCTTAAAAAGAAATTATCCATGTTCTGATTCGGGAGCAAAACATCATTCCTTATATATTAATTGTGGTGGAAGGGAGATTAATATTGGTGGGGTTAAGTATCAAGCTGATACAGAACAAAAAGGTGCTTCATTCTATTACTCGTCTCAGAACCAGAACTGGGCCTTCAGCAGTACAGGGAACTTCATGGACAATGATGTTGATGCAGATATCTACATTAAATCCAATACATCTGCACTTTCCAATGTGTCTGTAATCGATTCAGAACTGTACACAACAGCACGCGCATCTGCCATTTCTCTTACATATTATGGACTCTGTCTCCTGAATGGAAATTATACTATCAAACTCCACTTTGCTGAGATTGTATTTACTAATGATAAATCATTTAATAGTCTTGGAGAGCGTATGTTCGATGTGTACATCCAGGATAAGCGGGTTCTAAAAGACTTCAATATAGAAAAGGAAGCTGGTGGTACTGGTAAGCGCATTATAAAGACATTCACTTCGATGGTTTCAAGTCATACACTCAAGATCCATTTTTACTGGGCTGGAAAAGGAACTACTGGCATCCCAGATAGAGGATTTTATGGTCCTCTCATATCAGCTATATCAGTAGATCCTAACTTTGAAGTTCCTTCAGATGATGGCAAAAAGAAGAAAGCCATAACATCAATTGGAATATCAACTGCAGCACTTCTGCTTCTGCTTTTGGTCGCAGGTATTATGTGGAGAAAGGGTTACATAGGAGGAAAGACCGCTGCAGATAAAGTCGTAATTAGTATCTTCTATCTCCTAAGATTCAAGTTTTGCCTTGTCCACAATGGTCTTTTAGCAGACGGCACAGTAATTGCAGTGAAGCAGCTCTCCTCGAAATCAAAGCAAGGAAATAGAGAATTTATAAATGAGATAGGCATGATTTCTGCCTTGCAACATCCAAACCTTGTGAAGCTGTATGGATGTTGTGTTGAAGGAAACCAGATGTTGTTAATTTACGAGTACATGGAGAATAATTGTGTATCGCATGCTCTTTTCGCGAGAGACCCTACTCGCAGATTGAAATTGGACTGGCCTACTAGGAAGAACATTTGCCTAGAGTCAATCTTTATTGCAACGAAAACTAAGGTGAAGCTACTGAGATATAACCTTGGTTATATGGCTCCTGAGTATGCCATGCGTGGCTACTTAACCGCAAAAGCAGATGTTTATAGCTTTGGAGTTGTGGCACTAGAAATTGTTAGCGGAAAGAGCAATACAAACTATAGGCCAAAGGAGGAATTCGTCTACCTTCTTGATTGGGCTTATGTTCTGCAAGAGAGGGGGAGTCTATTGGAGTTGGTCGATCCAGCCTTGGGTTCAGAGTATTCATCAGAGGAGACAATGGTGATGCTAAATGTTGCTCTCTTGTGCACCAATGCCTCACCAACTCTAAGGCCTAAAATGTCCCAAGTTGTGAGCATGCTTGAAGGAAAAACAGAAGTTCAAGACCTTCTTTCTGATCCTGGCTTTTCAGCCGTTAATTCCAAGTACAAGGCCATCAGAAATCACTTCTGGCTACATCCAAGCCGTACTCAAAGCATGTCAGTCATGGACCGTACCGATACCTCTGGTTCATATAACGAATCAGAAGAGAGTGGCCATCTTTTAAGCGTGAGTTCAGTTAAAGCTGATGAATACATTGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

969

Amino Acids

107.52

Weight (kDa)

8.41

Isoelectric Point (pI)

29.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	166 - 274	3.2e-06	Leucine-rich repeat region
LRR_8	PF13855	292 - 350	3.8e-08	Leucine rich repeat
Malectin	PF11721	425 - 611	1.1e-41	Malectin domain
PK_Tyr_Ser-Thr	PF07714	677 - 755	2.8e-12	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	686 - 750	1.4e-08	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	786 - 895	3.1e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07650 AT1G07650 AT1G07650 AT1G07650
fragaria_vesca	FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550
malus_domestica	MD09G1156900.v1.1
prunus_persica	Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1
pyrus_communis	pycom09g07560 pycom11g23700
rosa_chinensis	RchiOBHm_Chr2g0149971 RchiOBHm_Chr2g0150431
rosa_laevigata	RLG00000000535 RLG00000001460 RLG00000011700 RLG00000016818 RLG00000020498 RLG00000030032 RLG00000034736
rosa_multiflora	Rmu_co8438829.1_g000001 Rmu_sc0020041.1_g000001 Rmu_ssc0000480.1_g000017
rosa_roxburghii	Rroxscaffold_2G00097110
rosa_rugosa	Rorug02G0421300
rosa_samantha	Rh2DG501600 Rh4AG126900
rosa_wichuraiana	Rw2G039240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1715, 2123
Acc36I	ACCTGC	1 cut(s)	1931
AccI	GTMKAC	1 cut(s)	2505
AccII	CGCG	2 cut(s)	1506, 2258
AciI	CCGC	6 cut(s)	851, 920, 1657, 1980, 2412, 2463
AclWI	GGATC	6 cut(s)	188, 910, 1750, 1835, 2558, 2714
AcoI	YGGCCR	1 cut(s)	2857
AcsI	RAATTY	4 cut(s)	328, 1168, 2117, 2498
AcuI	CTGAAG	4 cut(s)	46, 104, 1372, 1845
AfiI	CCNNNNNNNGG	6 cut(s)	203, 763, 764, 1381, 1802, 2573
AjnI	CCWGG	4 cut(s)	126, 1063, 1647, 2722
Alw21I	GWGCWC	2 cut(s)	257, 2633
Alw26I	GTCTC	4 cut(s)	1371, 1547, 2256, 2591
Alw44I	GTGCAC	1 cut(s)	2629
AlwI	GGATC	6 cut(s)	188, 910, 1750, 1835, 2558, 2714
AoxI	GGCC	8 cut(s)	3, 551, 1383, 2291, 2486, 2654, 2754, 2857
ApaI	GGGCCC	1 cut(s)	555
ApaLI	GTGCAC	1 cut(s)	2629
ApeKI	GCWGC	5 cut(s)	104, 273, 1913, 1982, 2083
ApoI	RAATTY	4 cut(s)	328, 1168, 2117, 2498
ArsI	GACNNNNNNTTYG	6 cut(s)	167, 199, 715, 747, 1711, 1743
AseI	ATTAAT	2 cut(s)	1281, 1302
AspLEI	GCGC	2 cut(s)	1508, 1710
AspS9I	GGNCC	8 cut(s)	4, 479, 551, 552, 767, 1383, 1814, 2812
AsuHPI	GGTGA	5 cut(s)	490, 610, 2359, 2617, 2634
AsuII	TTCGAA	1 cut(s)	734
AvaII	GGWCC	4 cut(s)	479, 767, 1814, 2812
BaeGI	GKGCMC	2 cut(s)	555, 2633
BalI	TGGCCA	1 cut(s)	2859
BanII	GRGCYC	2 cut(s)	257, 555
BarI	GAAGNNNNNNTAC	2 cut(s)	826, 858
BbsI	GAAGAC	1 cut(s)	834
Bbv12I	GWGCWC	2 cut(s)	257, 2633
BbvCI	CCTCAGC	1 cut(s)	962
BbvI	GCAGC	5 cut(s)	116, 285, 1925, 1969, 2095
BccI	CCATC	7 cut(s)	4, 227, 1062, 1726, 1862, 2765, 2868
BceAI	ACGGC	3 cut(s)	2077, 2720, 2772
BciT130I	CCWGG	4 cut(s)	128, 1065, 1649, 2724
BclI	TGATCA	1 cut(s)	651
BcoDI	GTCTC	4 cut(s)	1371, 1547, 2256, 2591
BfaI	CTAG	6 cut(s)	452, 641, 780, 2298, 2315, 2450
BfmI	CTRYAG	3 cut(s)	1911, 1983, 2482
BfuAI	ACCTGC	1 cut(s)	1931
BisI	GCNGC	5 cut(s)	105, 274, 1914, 1983, 2084
BlpI	GCTNAGC	1 cut(s)	114
BlsI	GCNGC	5 cut(s)	106, 275, 1915, 1984, 2085
Bme1390I	CCNGG	4 cut(s)	128, 1065, 1649, 2724
Bme18I	GGWCC	4 cut(s)	479, 767, 1814, 2812
BmgT120I	GGNCC	8 cut(s)	4, 479, 551, 552, 767, 1383, 1814, 2812
BmiI	GGNNCC	5 cut(s)	412, 480, 553, 768, 2382
BmrFI	CCNGG	4 cut(s)	128, 1065, 1649, 2724
BmrI	ACTGGG	3 cut(s)	187, 1390, 1777
BmsI	GCATC	4 cut(s)	1414, 1517, 1800, 2598
BmuI	ACTGGG	3 cut(s)	187, 1390, 1777
BpiI	GAAGAC	1 cut(s)	834
Bpu10I	CCTNAGC	1 cut(s)	962
Bpu1102I	GCTNAGC	1 cut(s)	114
Bpu14I	TTCGAA	1 cut(s)	734
BpuEI	CTTGAG	3 cut(s)	544, 1114, 1736
Bsa29I	ATCGAT	1 cut(s)	1479
BsaAI	YACGTR	1 cut(s)	301
BsaI	GGTCTC	1 cut(s)	2256
BsaJI	CCNNGG	3 cut(s)	35, 2368, 2571
BsaWI	WCCGGW	1 cut(s)	620
BsaXI	ACNNNNNCTCC	4 cut(s)	1527, 1557, 1945, 1975
Bsc4I	CCNNNNNNNGG	6 cut(s)	203, 763, 764, 1381, 1802, 2573
BseBI	CCWGG	4 cut(s)	128, 1065, 1649, 2724
BseCI	ATCGAT	1 cut(s)	1479
BseDI	CCNNGG	3 cut(s)	35, 2368, 2571
BseGI	GGATG	7 cut(s)	243, 1073, 1644, 1791, 2155, 2186, 2779
BseLI	CCNNNNNNNGG	6 cut(s)	203, 763, 764, 1381, 1802, 2573
BseMII	CTCAG	8 cut(s)	529, 803, 881, 976, 1382, 1572, 2348, 2376
BseRI	GAGGAG	2 cut(s)	2080, 2609
BseSI	GKGCMC	2 cut(s)	555, 2633
BseXI	GCAGC	5 cut(s)	116, 285, 1925, 1969, 2095
BsgI	GTGCAG	2 cut(s)	1005, 1440
Bsh1236I	CGCG	2 cut(s)	1506, 2258
BshFI	GGCC	8 cut(s)	5, 553, 1385, 2293, 2488, 2656, 2756, 2859
BshVI	ATCGAT	1 cut(s)	1479
BsiHKAI	GWGCWC	2 cut(s)	257, 2633
BsiSI	CCGG	1 cut(s)	621
BslFI	GGGAC	1 cut(s)	2650
BslI	CCNNNNNNNGG	6 cut(s)	203, 763, 764, 1381, 1802, 2573
BsmAI	GTCTC	4 cut(s)	1371, 1547, 2256, 2591
BsmBI	CGTCTC	1 cut(s)	1371
BsmFI	GGGAC	1 cut(s)	2650
BsmI	GAATGC	1 cut(s)	142
BsnI	GGCC	8 cut(s)	5, 553, 1385, 2293, 2488, 2656, 2756, 2859
Bso31I	GGTCTC	1 cut(s)	2256
Bsp119I	TTCGAA	1 cut(s)	734
Bsp120I	GGGCCC	1 cut(s)	551
Bsp1286I	GDGCHC	3 cut(s)	257, 555, 2633
Bsp1407I	TGTACA	2 cut(s)	1491, 1641
Bsp143I	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
Bsp1720I	GCTNAGC	1 cut(s)	114
Bsp68I	TCGCGA	1 cut(s)	2258
BspACI	CCGC	6 cut(s)	851, 920, 1657, 1980, 2412, 2463
BspANI	GGCC	8 cut(s)	5, 553, 1385, 2293, 2488, 2656, 2756, 2859
BspCNI	CTCAG	8 cut(s)	528, 804, 882, 975, 1381, 1573, 2349, 2377
BspDI	ATCGAT	1 cut(s)	1479
BspFNI	CGCG	2 cut(s)	1506, 2258
BspLI	GGNNCC	5 cut(s)	412, 480, 553, 768, 2382
BspMAI	CTGCAG	2 cut(s)	1915, 1987
BspMI	ACCTGC	1 cut(s)	1931
BspPI	GGATC	6 cut(s)	188, 910, 1750, 1835, 2558, 2714
BspT104I	TTCGAA	1 cut(s)	734
BspTNI	GGTCTC	1 cut(s)	2256
BsrGI	TGTACA	2 cut(s)	1491, 1641
BssECI	CCNNGG	3 cut(s)	35, 2368, 2571
BssMI	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
BssT1I	CCWWGG	3 cut(s)	35, 2368, 2571
Bst2UI	CCWGG	4 cut(s)	128, 1065, 1649, 2724
Bst4CI	ACNGT	4 cut(s)	1093, 1491, 2068, 2816
Bst6I	CTCTTC	1 cut(s)	2844
BstAUI	TGTACA	2 cut(s)	1491, 1641
BstBAI	YACGTR	1 cut(s)	301
BstBI	TTCGAA	1 cut(s)	734
BstC8I	GCNNGC	3 cut(s)	1504, 2247, 2681
BstENI	CCTNNNNNAGG	1 cut(s)	201
BstF5I	GGATG	7 cut(s)	243, 1073, 1644, 1791, 2155, 2186, 2779
BstFNI	CGCG	2 cut(s)	1506, 2258
BstHHI	GCGC	2 cut(s)	1508, 1710
BstKTI	GATC	8 cut(s)	196, 654, 811, 918, 1758, 1843, 2566, 2722
BstMAI	GTCTC	4 cut(s)	1371, 1547, 2256, 2591
BstMBI	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
BstMWI	GCNNNNNNNGC	5 cut(s)	110, 252, 320, 2255, 2469
BstNI	CCWGG	4 cut(s)	128, 1065, 1649, 2724
BstNSI	RCATGY	4 cut(s)	1224, 2249, 2683, 2803
BstSCI	CCNGG	4 cut(s)	126, 1063, 1647, 2722
BstSFI	CTRYAG	3 cut(s)	1911, 1983, 2482
BstSLI	GKGCMC	2 cut(s)	555, 2633
BstUI	CGCG	2 cut(s)	1506, 2258
BstV1I	GCAGC	5 cut(s)	116, 285, 1925, 1969, 2095
BstV2I	GAAGAC	1 cut(s)	834
BstX2I	RGATCY	4 cut(s)	193, 915, 1755, 1840
BstXI	CCANNNNNNTGG	2 cut(s)	442, 562
BstYI	RGATCY	4 cut(s)	193, 915, 1755, 1840
Bsu15I	ATCGAT	1 cut(s)	1479
BsuRI	GGCC	8 cut(s)	5, 553, 1385, 2293, 2488, 2656, 2756, 2859
BsuTUI	ATCGAT	1 cut(s)	1479
BtsCI	GGATG	7 cut(s)	243, 1073, 1644, 1791, 2155, 2186, 2779
BtsI	GCAGTG	3 cut(s)	69, 341, 2082
BtsIMutI	CAGTG	4 cut(s)	69, 341, 1098, 2082
BtuMI	TCGCGA	1 cut(s)	2258
BveI	ACCTGC	1 cut(s)	1931
Cac8I	GCNNGC	3 cut(s)	1504, 2247, 2681
CfoI	GCGC	2 cut(s)	1508, 1710
Cfr13I	GGNCC	8 cut(s)	4, 479, 551, 552, 767, 1383, 1814, 2812
ClaI	ATCGAT	1 cut(s)	1479
CseI	GACGC	1 cut(s)	733
DpnI	GATC	8 cut(s)	195, 653, 810, 917, 1757, 1842, 2565, 2721
DpnII	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
EaeI	YGGCCR	1 cut(s)	2857
Eam1104I	CTCTTC	1 cut(s)	2844
EarI	CTCTTC	1 cut(s)	2844
Ecl136II	GAGCTC	1 cut(s)	255
Eco130I	CCWWGG	3 cut(s)	35, 2368, 2571
Eco147I	AGGCCT	1 cut(s)	2656
Eco24I	GRGCYC	2 cut(s)	257, 555
Eco31I	GGTCTC	1 cut(s)	2256
Eco32I	GATATC	1 cut(s)	1432
Eco47I	GGWCC	4 cut(s)	479, 767, 1814, 2812
Eco53kI	GAGCTC	1 cut(s)	255
Eco57I	CTGAAG	4 cut(s)	46, 104, 1372, 1845
EcoICRI	GAGCTC	1 cut(s)	255
EcoNI	CCTNNNNNAGG	1 cut(s)	201
EcoO109I	RGGNCCY	2 cut(s)	551, 767
EcoRI	GAATTC	1 cut(s)	2498
EcoRII	CCWGG	4 cut(s)	126, 1063, 1647, 2722
EcoRV	GATATC	1 cut(s)	1432
EcoT14I	CCWWGG	3 cut(s)	35, 2368, 2571
EcoT22I	ATGCAT	1 cut(s)	142
EcoT38I	GRGCYC	2 cut(s)	257, 555
ErhI	CCWWGG	3 cut(s)	35, 2368, 2571
Esp3I	CGTCTC	1 cut(s)	1371
FalI	AAGNNNNNCTT	4 cut(s)	525, 557, 2697, 2729
FaqI	GGGAC	1 cut(s)	2650
FauI	CCCGC	3 cut(s)	858, 927, 1650
FbaI	TGATCA	1 cut(s)	651
FblI	GTMKAC	1 cut(s)	2505
Fnu4HI	GCNGC	5 cut(s)	105, 274, 1914, 1983, 2084
FokI	GGATG	7 cut(s)	250, 1080, 1631, 1778, 2142, 2193, 2766
FriOI	GRGCYC	2 cut(s)	257, 555
Fsp4HI	GCNGC	5 cut(s)	105, 274, 1914, 1983, 2084
FspBI	CTAG	6 cut(s)	452, 641, 780, 2298, 2315, 2450
GlaI	GCGC	2 cut(s)	1507, 1709
GluI	GCNGC	5 cut(s)	105, 274, 1914, 1983, 2084
HaeIII	GGCC	8 cut(s)	5, 553, 1385, 2293, 2488, 2656, 2756, 2859
HapII	CCGG	1 cut(s)	621
HgaI	GACGC	1 cut(s)	733
HhaI	GCGC	2 cut(s)	1508, 1710
Hin6I	GCGC	2 cut(s)	1506, 1708
HinP1I	GCGC	2 cut(s)	1506, 1708
HincII	GTYRAC	1 cut(s)	978
HindII	GTYRAC	1 cut(s)	978
HindIII	AAGCTT	4 cut(s)	116, 244, 362, 1046
HinfI	GANTC	9 cut(s)	1083, 1212, 1250, 1481, 1536, 2022, 2318, 2545, 2843
HpaII	CCGG	1 cut(s)	621
HphI	GGTGA	5 cut(s)	490, 610, 2359, 2617, 2634
Hpy166II	GTNNAC	7 cut(s)	341, 978, 1494, 1642, 2041, 2506, 2631
Hpy8I	GTNNAC	7 cut(s)	341, 978, 1494, 1642, 2041, 2506, 2631
HpyCH4III	ACNGT	4 cut(s)	1093, 1491, 2068, 2816
HpyCH4IV	ACGT	1 cut(s)	300
HpyF10VI	GCNNNNNNNGC	5 cut(s)	110, 252, 320, 2255, 2469
HpySE526I	ACGT	1 cut(s)	300
HspAI	GCGC	2 cut(s)	1506, 1708
Ksp22I	TGATCA	1 cut(s)	651
Kzo9I	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
LmnI	GCTCC	2 cut(s)	1256, 2386
Lsp1109I	GCAGC	5 cut(s)	116, 285, 1925, 1969, 2095
LweI	GCATC	4 cut(s)	1414, 1517, 1800, 2598
MaeI	CTAG	6 cut(s)	452, 641, 780, 2298, 2315, 2450
MaeII	ACGT	1 cut(s)	300
MaeIII	GTNAC	2 cut(s)	259, 1961
MalI	GATC	8 cut(s)	195, 653, 810, 917, 1757, 1842, 2565, 2721
MboI	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
MboII	GAAGA	7 cut(s)	52, 181, 834, 1891, 1999, 2314, 2861
MfeI	CAATTG	1 cut(s)	1896
MflI	RGATCY	4 cut(s)	193, 915, 1755, 1840
MhlI	GDGCHC	3 cut(s)	257, 555, 2633
MlsI	TGGCCA	1 cut(s)	2859
MluNI	TGGCCA	1 cut(s)	2859
MlyI	GAGTC	4 cut(s)	1221, 1530, 2327, 2554
MmeI	TCCRAC	2 cut(s)	423, 543
Mox20I	TGGCCA	1 cut(s)	2859
Mph1103I	ATGCAT	1 cut(s)	142
MscI	TGGCCA	1 cut(s)	2859
MslI	CAYNNNNRTG	1 cut(s)	1730
Msp20I	TGGCCA	1 cut(s)	2859
MspA1I	CMGCKG	2 cut(s)	1196, 1982
MspI	CCGG	1 cut(s)	621
MspR9I	CCNGG	4 cut(s)	128, 1065, 1649, 2724
MunI	CAATTG	1 cut(s)	1896
Mva1269I	GAATGC	1 cut(s)	142
MvaI	CCWGG	4 cut(s)	128, 1065, 1649, 2724
MvnI	CGCG	2 cut(s)	1506, 2258
MwoI	GCNNNNNNNGC	5 cut(s)	110, 252, 320, 2255, 2469
NdeII	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
NlaIV	GGNNCC	5 cut(s)	412, 480, 553, 768, 2382
NruI	TCGCGA	1 cut(s)	2258
NsiI	ATGCAT	1 cut(s)	142
NspI	RCATGY	4 cut(s)	1224, 2249, 2683, 2803
NspV	TTCGAA	1 cut(s)	734
PaeI	GCATGC	2 cut(s)	2249, 2683
PceI	AGGCCT	1 cut(s)	2656
PcsI	WCGNNNNNNNCGW	1 cut(s)	731
PctI	GAATGC	1 cut(s)	142
PfeI	GAWTC	5 cut(s)	1083, 1250, 1481, 2022, 2843
PflFI	GACNNNGTC	1 cut(s)	1539
PfoI	TCCNGGA	1 cut(s)	1647
PkrI	GCNGC	5 cut(s)	106, 275, 1915, 1984, 2085
PleI	GAGTC	4 cut(s)	1220, 1530, 2326, 2553
PpsI	GAGTC	4 cut(s)	1220, 1530, 2326, 2553
Ppu21I	YACGTR	1 cut(s)	301
PpuMI	RGGWCCY	1 cut(s)	767
PshBI	ATTAAT	2 cut(s)	1281, 1302
PsiI	TTATAA	2 cut(s)	1715, 2123
Psp124BI	GAGCTC	1 cut(s)	257
Psp5II	RGGWCCY	1 cut(s)	767
Psp6I	CCWGG	4 cut(s)	126, 1063, 1647, 2722
PspGI	CCWGG	4 cut(s)	126, 1063, 1647, 2722
PspN4I	GGNNCC	5 cut(s)	412, 480, 553, 768, 2382
PspOMI	GGGCCC	1 cut(s)	551
PspPI	GGNCC	8 cut(s)	4, 479, 551, 552, 767, 1383, 1814, 2812
PspPPI	RGGWCCY	1 cut(s)	767
PstI	CTGCAG	2 cut(s)	1915, 1987
PsuI	RGATCY	4 cut(s)	193, 915, 1755, 1840
PsyI	GACNNNGTC	1 cut(s)	1539
PvuII	CAGCTG	1 cut(s)	1196
RruI	TCGCGA	1 cut(s)	2258
RseI	CAYNNNNRTG	1 cut(s)	1730
SacI	GAGCTC	1 cut(s)	257
SatI	GCNGC	5 cut(s)	105, 274, 1914, 1983, 2084
Sau3AI	GATC	8 cut(s)	193, 651, 808, 915, 1755, 1840, 2563, 2719
Sau96I	GGNCC	8 cut(s)	4, 479, 551, 552, 767, 1383, 1814, 2812
SchI	GAGTC	4 cut(s)	1221, 1530, 2327, 2554
ScrFI	CCNGG	4 cut(s)	128, 1065, 1649, 2724
SduI	GDGCHC	3 cut(s)	257, 555, 2633
SfaNI	GCATC	4 cut(s)	1414, 1517, 1800, 2598
SfcI	CTRYAG	3 cut(s)	1911, 1983, 2482
SfuI	TTCGAA	1 cut(s)	734
SinI	GGWCC	4 cut(s)	479, 767, 1814, 2812
SmiMI	CAYNNNNRTG	1 cut(s)	1730
SmlI	CTYRAG	3 cut(s)	523, 1129, 1751
SmoI	CTYRAG	3 cut(s)	523, 1129, 1751
SphI	GCATGC	2 cut(s)	2249, 2683
SseBI	AGGCCT	1 cut(s)	2656
SsiI	CCGC	6 cut(s)	851, 920, 1657, 1980, 2412, 2463
SspI	AATATT	2 cut(s)	505, 1306
SspMI	CTAG	6 cut(s)	452, 641, 780, 2298, 2315, 2450
SstI	GAGCTC	1 cut(s)	257
StuI	AGGCCT	1 cut(s)	2656
StyD4I	CCNGG	4 cut(s)	126, 1063, 1647, 2722
StyI	CCWWGG	3 cut(s)	35, 2368, 2571
TaaI	ACNGT	4 cut(s)	1093, 1491, 2068, 2816
TaiI	ACGT	1 cut(s)	303
TaqI	TCGA	6 cut(s)	734, 1479, 1635, 1730, 2093, 2562
TatI	WGTACW	5 cut(s)	1394, 1491, 1641, 2220, 2748
TfiI	GAWTC	5 cut(s)	1083, 1250, 1481, 2022, 2843
TscAI	CASTG	4 cut(s)	76, 348, 1098, 2082
TseI	GCWGC	5 cut(s)	104, 273, 1913, 1982, 2083
TspGWI	ACGGA	1 cut(s)	549
TspRI	CASTG	4 cut(s)	76, 348, 1098, 2082
Tth111I	GACNNNGTC	1 cut(s)	1539
VneI	GTGCAC	1 cut(s)	2629
VpaK11BI	GGWCC	4 cut(s)	479, 767, 1814, 2812
VspI	ATTAAT	2 cut(s)	1281, 1302
XagI	CCTNNNNNAGG	1 cut(s)	201
XapI	RAATTY	4 cut(s)	328, 1168, 2117, 2498
XceI	RCATGY	4 cut(s)	1224, 2249, 2683, 2803
XmiI	GTMKAC	1 cut(s)	2505
XspI	CTAG	6 cut(s)	452, 641, 780, 2298, 2315, 2450
Zsp2I	ATGCAT	1 cut(s)	142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.