RchiOBHm_Chr2g0150431

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001943
Physical Location & Seq
Forward (+)
68106116 .. 68113357
7242 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51977

Sequence Viewer

Length: 3066 bp
ATGGCCCAGATGGGAACTGACCATTTTTCATTCCTAAGGTTGCTTCTTGCATATCTCATCTTCAGTTTCACTGCATTTTTCTTCCCTGCAAGTTTTGGACAAGCAGCCACAGCTAAGCTTCAGAGCCAGGAAGTGAATGCATTGAAAGAGATAGCAAAGAAGTTGGGGAAGAAAGACTGGGATTTTGGGAAAGATCCTTGCACAGGAGAAGGGAATTGGAGAGTTTTTGTAGATGGGATGAAAAGCTTTGAGAGCTCTGTAACTTGTGATTGCAGCTTCAACAACAATTCCTCTTGCCACGTAGTCAGCATAGCTTTGAAGGCACAGAATTTAACTGGTACACTGCCACCAGAGTTCTCAAAGCTTCATTACCTCAAAGTATTAGATGTGAGTAGAAACCTCCTTACTGGTTCCATACCTTCTCAATGGGCAACCATGCAGTTGGTGGAACTTGGTCTTATGGGGAACAGATTGTCGGGTCCATTTCCAAAAGTTCTCACCAATATTACCACCCTCAGAAACTTGAGCATAGAAGGAAACCTTTTTTCAGGGCCCATTCCGTTGGAAATTGGAAAGTTGGTCAAGTTAGAAAAGCTCATTTTATCGTCAAATCTATTCACCGGAGAATTGCCACAAGCACTAGCAAAGTTGACCAATTTGCTTGACATGAGGATAACTGACAACAACTTCTCTGGAAAGATACCTGACTTCATTGGAAACTGGACTCAGATTTCAAAATTGCTAATTCAAGGTTGCCCTTTTGAGGGTCCTATACCTTCTAGTATATCAGCCCTAACAGGCTTGATTGATCTGAGGATAACCGATTTAAAAGGTAGAGGGTCTTCTTTCCCACCATTGAGTGAAATGGCTTCCTTGAAAACATTGGTACTGAGGAACTGCTTAATATATGGAGAGATCCCGCATTACATTGGCGATATGAAAAGATTGAAAAACCTTGACCTCAGCTATAATGAGTTGACCGGTGCAGTACCAGTATCGTTTGACAAACTGACAAAAGTTGATTTCATATATTTAACAGCAAATAAGCTTACTGGAACTATACCTGGATGGGTCTTGAAACGAATCAAGACAGTGGATTTTTCTTACAACAACTTCACTTGGGAAAGCTCAAGCTCACTTCCTACTGAATGTGATAGAGGGAGTGTAAATTTAGTAGAGAGCTATTCCTCATCAGCTGATAATATAAGTAGAGTCGCAACATGCTTAAAAAGAAATTATCCATGTTCTGCTTCGGGAGAAAAACATCATTCCTTACATATTAATTGTGGTGGAAGGGAGATAAATATTGGTGGGGTTAAGTATCAAGCTGATACAGAACAAAAAGGTGCTTCATTCTATTACTCGTCTCAGAACCAGAACTGGGCCTTCAGCAGTACAGGGAACTTCATGGATAATGATGTTGATGCAGATATCTACATTAAATCCAATACATCTGCACTTTCCAATGTGCCTGTTATCGATTCAGAACTGTACACAACAGCACGCGCATCTGCCATATCTCTGACATATTATGGACTCTGTCTCCTGAATGGAAATTATACTATCAAACTCCACTTTGCTGAGATTGTATTTACTAATGATAAATCATTTAATAGTCTTGGAGAGCGTATGTTCGATGTGTACATCCAGGATAAGCGGGTTCTAAAAGACTTCAATATAGAAAAGGAATCTGGTGGTACTGGTAAGCGCATTATAAAGACATTCACTTCAATGGTTTCAAGTCATACACTCAAGATCCATTTTTACTGGGCTGGAAAAGGAACTACTGGCATCCCAGATAGAGGGTTTTATGGTCCTCTCATATCAGCTATATCAGTAAATCCTAACTTTGAAGTTCCTTCAGATGATGGCAAAAAGAAGAAAGCCATAACATCAATTGGAATATCAACTGCAGCACTTCTGCTTCTGCTTTTGGTCGCAGGTATTATGTGGAGAAAGGGCTACATAGGAGGAAAGATCGCTGCAGATAAAGAGCTCAGAGAAATGGATTTGCAAACAGGATTTTTTACTTTAAAACAGATAAAAGCTGCGACAAAAAACTTTGATGCAGCAAACAAACTTGGGGAAGGTGGTTTTGGTTCGGTCTACAAGGGTCTTTTAGCAGATGGCACAGTAATTGCAGTGAAGCAGCTCTCCTCAAAATCAAAGCAAGGAAATCGAGAATTTATAAATGAGATAGGCATGATTTCTGCCTTGCAACATCCAAACCTTGTGAAGCTGTATGGATGTTGTGTTGAAGGAAACCAGATGTTGTTAATTTACGAGTACATGGAGAATAACTGTGTATCACGTGCTCTTTTCGCGAGAGACCCTACTTGCAGATTGAAATTGGACTGGCCTACTAGGAAGAACATTTGCCTAGGTATTGCTAGAGGTTTGGCCTATCTACATGAAGAATCCAGAATAAGAATAGTGCATCGCGATATCAAGACGAGCAATGTGTTGCTTGATAAGAATTTCAATGCTAAAATCTCTGATTTTGGCTTGGCAAAGCTTAATGAGGATGGCAACACCCACATTAGCACTCGGATTGCTGGAACCATTGGTTATATGGCTCCTGAGTATGCCATGCGTGGCTACTTAACCGCAAAAGCAGATGTTTATAGCTTTGGAGTTGTGGCACTAGAAATTGTTAGCGGAAAGAGCAATACAAACTATAGGCCAAAGGAGGAATTCGTCTACCTTCTTGATTGGGCTTATGTTCTGCAAGAGAGGGGGAGTCTATTGGAGTTGGTTGATCCAGCCTTGGGTTCAGAATATTCGTCAGAGGAGACAATGGTGATGCTAAATGTTGCTCTCTTATGCACCAATGCCTCTCCAACTCTAAGGCCTAAAATGTCTCAAGTTGTGAGCATGCTTGAAGGAAAAACAGAAGTTCAAGACCTTCTTTCTGATCCTGGCTTTTCAGCAGTTAATTCCAAGTACAAGGCCATCAGAAATCACTTCTGGTTACATCCAAGCCGTACTCAAACCATGTCAATCAATGGTCCATGTACCGGTACCTCTGGTTCATATAACGAATCAGAAGAGAGTGGCCATCTTTAA

Protein Analysis

1021

Amino Acids

112.73

Weight (kDa)

8.59

Isoelectric Point (pI)

28.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 166 - 273 1.4e-06 Leucine-rich repeat region
LRR_8 PF13855 291 - 350 1.2e-08 Leucine rich repeat
Malectin PF11721 424 - 611 1.5e-41 Malectin domain
Pkinase PF00069 690 - 892 5.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 691 - 960 9.9e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1715, 2189
Acc36I ACCTGC 1 cut(s) 1931
Acc65I GGTACC 1 cut(s) 3020
AccB1I GGYRCC 1 cut(s) 3020
AccI GTMKAC 2 cut(s) 2106, 2700
AccII CGCG 3 cut(s) 1506, 2324, 2442
AciI CCGC 4 cut(s) 920, 1657, 2607, 2658
AclWI GGATC 5 cut(s) 188, 910, 1750, 2753, 2909
AcoI YGGCCR 1 cut(s) 3055
AcsI RAATTY 5 cut(s) 328, 1168, 2183, 2476, 2693
AcuI CTGAAG 4 cut(s) 46, 104, 1372, 1845
AcvI CACGTG 1 cut(s) 2312
AfiI CCNNNNNNNGG 7 cut(s) 203, 763, 764, 1381, 1802, 2768, 3017
AgeI ACCGGT 2 cut(s) 980, 3017
AjnI CCWGG 4 cut(s) 126, 1063, 1647, 2917
AjuI GAANNNNNNNTTGG 2 cut(s) 2079, 2111
Alw21I GWGCWC 3 cut(s) 257, 1998, 2317
Alw26I GTCTC 5 cut(s) 1371, 1547, 2322, 2786, 2865
AlwI GGATC 5 cut(s) 188, 910, 1750, 2753, 2909
AoxI GGCC 9 cut(s) 3, 551, 1383, 2357, 2400, 2681, 2849, 2949, 3055
ApaI GGGCCC 1 cut(s) 555
ApeKI GCWGC 7 cut(s) 104, 273, 1913, 1982, 2048, 2069, 2149
ApoI RAATTY 5 cut(s) 328, 1168, 2183, 2476, 2693
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 1 cut(s) 1281
AsiGI ACCGGT 2 cut(s) 980, 3017
Asp718I GGTACC 1 cut(s) 3020
AspA2I CCTAGG 1 cut(s) 2380
AspLEI GCGC 2 cut(s) 1508, 1710
AspS9I GGNCC 8 cut(s) 4, 479, 551, 552, 767, 1383, 1814, 3008
AsuHPI GGTGA 3 cut(s) 490, 610, 2812
AvaII GGWCC 4 cut(s) 479, 767, 1814, 3008
AvrII CCTAGG 1 cut(s) 2380
AxyI CCTNAGG 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 555
BalI TGGCCA 1 cut(s) 3057
BanI GGYRCC 1 cut(s) 3020
BanII GRGCYC 3 cut(s) 257, 555, 1998
BarI GAAGNNNNNNTAC 2 cut(s) 826, 858
BbrPI CACGTG 1 cut(s) 2312
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 3 cut(s) 257, 1998, 2317
BbvCI CCTCAGC 1 cut(s) 962
BbvI GCAGC 7 cut(s) 116, 285, 1925, 1969, 2035, 2081, 2161
BccI CCATC 7 cut(s) 4, 227, 1062, 1862, 2120, 2519, 2960
BceAI ACGGC 1 cut(s) 2967
BcgI CGANNNNNNTGC 2 cut(s) 2159, 2193
BciT130I CCWGG 4 cut(s) 128, 1065, 1649, 2919
BcoDI GTCTC 5 cut(s) 1371, 1547, 2322, 2786, 2865
BfaI CTAG 6 cut(s) 641, 780, 2364, 2381, 2391, 2645
BfmI CTRYAG 3 cut(s) 1911, 1983, 2677
BfuAI ACCTGC 1 cut(s) 1931
BisI GCNGC 7 cut(s) 105, 274, 1914, 1983, 2049, 2070, 2150
BlnI CCTAGG 1 cut(s) 2380
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 7 cut(s) 106, 275, 1915, 1984, 2050, 2071, 2151
Bme1390I CCNGG 4 cut(s) 128, 1065, 1649, 2919
Bme18I GGWCC 4 cut(s) 479, 767, 1814, 3008
BmgT120I GGNCC 8 cut(s) 4, 479, 551, 552, 767, 1383, 1814, 3008
BmiI GGNNCC 7 cut(s) 412, 480, 553, 768, 2560, 2577, 3022
BmrFI CCNGG 4 cut(s) 128, 1065, 1649, 2919
BmrI ACTGGG 3 cut(s) 187, 1390, 1777
BmsI GCATC 6 cut(s) 1414, 1517, 1800, 2056, 2446, 2793
BmuI ACTGGG 3 cut(s) 187, 1390, 1777
BpiI GAAGAC 1 cut(s) 834
Bpu10I CCTNAGC 1 cut(s) 962
Bpu1102I GCTNAGC 1 cut(s) 114
BpuEI CTTGAG 4 cut(s) 544, 1114, 1736, 2847
Bsa29I ATCGAT 1 cut(s) 1479
BsaAI YACGTR 2 cut(s) 301, 2312
BsaI GGTCTC 1 cut(s) 2322
BsaJI CCNNGG 2 cut(s) 2380, 2766
BsaWI WCCGGW 3 cut(s) 620, 980, 3017
BsaXI ACNNNNNCTCC 2 cut(s) 1527, 1557
Bsc4I CCNNNNNNNGG 7 cut(s) 203, 763, 764, 1381, 1802, 2768, 3017
Bse118I RCCGGY 2 cut(s) 980, 3017
Bse21I CCTNAGG 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 2464
BseBI CCWGG 4 cut(s) 128, 1065, 1649, 2919
BseCI ATCGAT 1 cut(s) 1479
BseDI CCNNGG 2 cut(s) 2380, 2766
BseGI GGATG 8 cut(s) 243, 1073, 1644, 1791, 2221, 2252, 2530, 2974
BseLI CCNNNNNNNGG 7 cut(s) 203, 763, 764, 1381, 1802, 2768, 3017
BseMI GCAATG 1 cut(s) 2464
BseMII CTCAG 9 cut(s) 529, 740, 803, 881, 976, 1382, 1572, 2011, 2571
BseRI GAGGAG 2 cut(s) 2146, 2804
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 7 cut(s) 116, 285, 1925, 1969, 2035, 2081, 2161
BsgI GTGCAG 2 cut(s) 1005, 1440
Bsh1236I CGCG 3 cut(s) 1506, 2324, 2442
BshFI GGCC 9 cut(s) 5, 553, 1385, 2359, 2402, 2683, 2851, 2951, 3057
BshNI GGYRCC 1 cut(s) 3020
BshTI ACCGGT 2 cut(s) 980, 3017
BshVI ATCGAT 1 cut(s) 1479
BsiHKAI GWGCWC 3 cut(s) 257, 1998, 2317
BsiSI CCGG 3 cut(s) 621, 981, 3018
BslI CCNNNNNNNGG 7 cut(s) 203, 763, 764, 1381, 1802, 2768, 3017
BsmAI GTCTC 5 cut(s) 1371, 1547, 2322, 2786, 2865
BsmBI CGTCTC 1 cut(s) 1371
BsmI GAATGC 1 cut(s) 142
BsnI GGCC 9 cut(s) 5, 553, 1385, 2359, 2402, 2683, 2851, 2951, 3057
Bso31I GGTCTC 1 cut(s) 2322
Bsp120I GGGCCC 1 cut(s) 551
Bsp1286I GDGCHC 4 cut(s) 257, 555, 1998, 2317
Bsp1407I TGTACA 2 cut(s) 1491, 1641
Bsp143I GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
Bsp1720I GCTNAGC 1 cut(s) 114
Bsp68I TCGCGA 2 cut(s) 2324, 2442
BspACI CCGC 4 cut(s) 920, 1657, 2607, 2658
BspANI GGCC 9 cut(s) 5, 553, 1385, 2359, 2402, 2683, 2851, 2951, 3057
BspCNI CTCAG 9 cut(s) 528, 739, 804, 882, 975, 1381, 1573, 2010, 2572
BspDI ATCGAT 1 cut(s) 1479
BspFNI CGCG 3 cut(s) 1506, 2324, 2442
BspLI GGNNCC 7 cut(s) 412, 480, 553, 768, 2560, 2577, 3022
BspMAI CTGCAG 2 cut(s) 1915, 1987
BspMI ACCTGC 1 cut(s) 1931
BspPI GGATC 5 cut(s) 188, 910, 1750, 2753, 2909
BspT107I GGYRCC 1 cut(s) 3020
BspTNI GGTCTC 1 cut(s) 2322
BsrDI GCAATG 1 cut(s) 2464
BsrFI RCCGGY 2 cut(s) 980, 3017
BsrGI TGTACA 2 cut(s) 1491, 1641
BssAI RCCGGY 2 cut(s) 980, 3017
BssECI CCNNGG 2 cut(s) 2380, 2766
BssMI GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
BssT1I CCWWGG 2 cut(s) 2380, 2766
Bst2UI CCWGG 4 cut(s) 128, 1065, 1649, 2919
Bst4CI ACNGT 4 cut(s) 1093, 1491, 2134, 2303
Bst6I CTCTTC 1 cut(s) 3042
BstAUI TGTACA 2 cut(s) 1491, 1641
BstBAI YACGTR 2 cut(s) 301, 2312
BstC8I GCNNGC 2 cut(s) 1504, 2876
BstENI CCTNNNNNAGG 1 cut(s) 201
BstF5I GGATG 8 cut(s) 243, 1073, 1644, 1791, 2221, 2252, 2530, 2974
BstFNI CGCG 3 cut(s) 1506, 2324, 2442
BstHHI GCGC 2 cut(s) 1508, 1710
BstKTI GATC 7 cut(s) 196, 811, 918, 1758, 1980, 2761, 2917
BstMAI GTCTC 5 cut(s) 1371, 1547, 2322, 2786, 2865
BstMBI GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
BstMWI GCNNNNNNNGC 5 cut(s) 110, 252, 320, 2321, 2664
BstNI CCWGG 4 cut(s) 128, 1065, 1649, 2919
BstNSI RCATGY 2 cut(s) 1224, 2878
BstSCI CCNGG 4 cut(s) 126, 1063, 1647, 2917
BstSFI CTRYAG 3 cut(s) 1911, 1983, 2677
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 3 cut(s) 1506, 2324, 2442
BstV1I GCAGC 7 cut(s) 116, 285, 1925, 1969, 2035, 2081, 2161
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 3 cut(s) 193, 915, 1755
BstXI CCANNNNNNTGG 2 cut(s) 442, 562
BstYI RGATCY 3 cut(s) 193, 915, 1755
Bsu15I ATCGAT 1 cut(s) 1479
Bsu36I CCTNAGG 1 cut(s) 35
BsuRI GGCC 9 cut(s) 5, 553, 1385, 2359, 2402, 2683, 2851, 2951, 3057
BsuTUI ATCGAT 1 cut(s) 1479
BtgZI GCGATG 1 cut(s) 2423
BtsCI GGATG 8 cut(s) 243, 1073, 1644, 1791, 2221, 2252, 2530, 2974
BtsI GCAGTG 3 cut(s) 69, 341, 2148
BtsIMutI CAGTG 4 cut(s) 69, 341, 1098, 2148
BtuMI TCGCGA 2 cut(s) 2324, 2442
BveI ACCTGC 1 cut(s) 1931
Cac8I GCNNGC 2 cut(s) 1504, 2876
CfoI GCGC 2 cut(s) 1508, 1710
Cfr10I RCCGGY 2 cut(s) 980, 3017
Cfr13I GGNCC 8 cut(s) 4, 479, 551, 552, 767, 1383, 1814, 3008
ClaI ATCGAT 1 cut(s) 1479
CspAI ACCGGT 2 cut(s) 980, 3017
DpnI GATC 7 cut(s) 195, 810, 917, 1757, 1979, 2760, 2916
DpnII GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
DraI TTTAAA 2 cut(s) 828, 2034
EaeI YGGCCR 1 cut(s) 3055
Eam1104I CTCTTC 1 cut(s) 3042
EarI CTCTTC 1 cut(s) 3042
Ecl136II GAGCTC 2 cut(s) 255, 1996
Eco130I CCWWGG 2 cut(s) 2380, 2766
Eco147I AGGCCT 1 cut(s) 2851
Eco24I GRGCYC 3 cut(s) 257, 555, 1998
Eco31I GGTCTC 1 cut(s) 2322
Eco32I GATATC 2 cut(s) 1432, 2446
Eco47I GGWCC 4 cut(s) 479, 767, 1814, 3008
Eco53kI GAGCTC 2 cut(s) 255, 1996
Eco57I CTGAAG 4 cut(s) 46, 104, 1372, 1845
Eco72I CACGTG 1 cut(s) 2312
Eco81I CCTNAGG 1 cut(s) 35
EcoICRI GAGCTC 2 cut(s) 255, 1996
EcoNI CCTNNNNNAGG 1 cut(s) 201
EcoO109I RGGNCCY 2 cut(s) 551, 767
EcoRI GAATTC 1 cut(s) 2693
EcoRII CCWGG 4 cut(s) 126, 1063, 1647, 2917
EcoRV GATATC 2 cut(s) 1432, 2446
EcoT14I CCWWGG 2 cut(s) 2380, 2766
EcoT22I ATGCAT 1 cut(s) 142
EcoT38I GRGCYC 3 cut(s) 257, 555, 1998
ErhI CCWWGG 2 cut(s) 2380, 2766
Esp3I CGTCTC 1 cut(s) 1371
FalI AAGNNNNNCTT 4 cut(s) 525, 557, 2892, 2924
FauI CCCGC 2 cut(s) 927, 1650
FblI GTMKAC 2 cut(s) 2106, 2700
Fnu4HI GCNGC 7 cut(s) 105, 274, 1914, 1983, 2049, 2070, 2150
FokI GGATG 8 cut(s) 250, 1080, 1631, 1778, 2208, 2259, 2537, 2961
FriOI GRGCYC 3 cut(s) 257, 555, 1998
Fsp4HI GCNGC 7 cut(s) 105, 274, 1914, 1983, 2049, 2070, 2150
FspBI CTAG 6 cut(s) 641, 780, 2364, 2381, 2391, 2645
GlaI GCGC 2 cut(s) 1507, 1709
GluI GCNGC 7 cut(s) 105, 274, 1914, 1983, 2049, 2070, 2150
HaeIII GGCC 9 cut(s) 5, 553, 1385, 2359, 2402, 2683, 2851, 2951, 3057
HapII CCGG 3 cut(s) 621, 981, 3018
HhaI GCGC 2 cut(s) 1508, 1710
Hin6I GCGC 2 cut(s) 1506, 1708
HinP1I GCGC 2 cut(s) 1506, 1708
HincII GTYRAC 2 cut(s) 651, 978
HindII GTYRAC 2 cut(s) 651, 978
HindIII AAGCTT 5 cut(s) 116, 244, 362, 1046, 2513
HinfI GANTC 9 cut(s) 724, 1083, 1212, 1481, 1536, 1688, 2417, 2740, 3041
HpaII CCGG 3 cut(s) 621, 981, 3018
HphI GGTGA 3 cut(s) 490, 610, 2812
Hpy166II GTNNAC 7 cut(s) 341, 651, 978, 1494, 1642, 2107, 2701
Hpy8I GTNNAC 7 cut(s) 341, 651, 978, 1494, 1642, 2107, 2701
HpyCH4III ACNGT 4 cut(s) 1093, 1491, 2134, 2303
HpyCH4IV ACGT 2 cut(s) 300, 2311
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 252, 320, 2321, 2664
HpySE526I ACGT 2 cut(s) 300, 2311
HspAI GCGC 2 cut(s) 1506, 1708
KpnI GGTACC 1 cut(s) 3024
Kzo9I GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
LmnI GCTCC 1 cut(s) 2581
Lsp1109I GCAGC 7 cut(s) 116, 285, 1925, 1969, 2035, 2081, 2161
LweI GCATC 6 cut(s) 1414, 1517, 1800, 2056, 2446, 2793
MaeI CTAG 6 cut(s) 641, 780, 2364, 2381, 2391, 2645
MaeII ACGT 2 cut(s) 300, 2311
MaeIII GTNAC 2 cut(s) 259, 2970
MalI GATC 7 cut(s) 195, 810, 917, 1757, 1979, 2760, 2916
MboI GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
MboII GAAGA 8 cut(s) 52, 73, 181, 834, 1891, 2380, 2426, 3059
MfeI CAATTG 1 cut(s) 1896
MflI RGATCY 3 cut(s) 193, 915, 1755
MhlI GDGCHC 4 cut(s) 257, 555, 1998, 2317
MlsI TGGCCA 1 cut(s) 3057
MluNI TGGCCA 1 cut(s) 3057
MlyI GAGTC 4 cut(s) 718, 1221, 1530, 2749
MmeI TCCRAC 2 cut(s) 543, 2864
Mox20I TGGCCA 1 cut(s) 3057
Mph1103I ATGCAT 1 cut(s) 142
MscI TGGCCA 1 cut(s) 3057
MslI CAYNNNNRTG 1 cut(s) 1730
Msp20I TGGCCA 1 cut(s) 3057
MspA1I CMGCKG 1 cut(s) 1196
MspI CCGG 3 cut(s) 621, 981, 3018
MspR9I CCNGG 4 cut(s) 128, 1065, 1649, 2919
MunI CAATTG 1 cut(s) 1896
Mva1269I GAATGC 1 cut(s) 142
MvaI CCWGG 4 cut(s) 128, 1065, 1649, 2919
MvnI CGCG 3 cut(s) 1506, 2324, 2442
MwoI GCNNNNNNNGC 5 cut(s) 110, 252, 320, 2321, 2664
NdeII GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
NlaIV GGNNCC 7 cut(s) 412, 480, 553, 768, 2560, 2577, 3022
NruI TCGCGA 2 cut(s) 2324, 2442
NsiI ATGCAT 1 cut(s) 142
NspI RCATGY 2 cut(s) 1224, 2878
PaeI GCATGC 1 cut(s) 2878
PceI AGGCCT 1 cut(s) 2851
PctI GAATGC 1 cut(s) 142
PfeI GAWTC 5 cut(s) 1083, 1481, 1688, 2417, 3041
PflFI GACNNNGTC 1 cut(s) 1539
PfoI TCCNGGA 1 cut(s) 1647
PinAI ACCGGT 2 cut(s) 980, 3017
PkrI GCNGC 7 cut(s) 106, 275, 1915, 1984, 2050, 2071, 2151
PleI GAGTC 4 cut(s) 718, 1220, 1530, 2748
PmaCI CACGTG 1 cut(s) 2312
PmlI CACGTG 1 cut(s) 2312
PpsI GAGTC 4 cut(s) 718, 1220, 1530, 2748
Ppu21I YACGTR 2 cut(s) 301, 2312
PpuMI RGGWCCY 1 cut(s) 767
PshBI ATTAAT 1 cut(s) 1281
PsiI TTATAA 2 cut(s) 1715, 2189
Psp124BI GAGCTC 2 cut(s) 257, 1998
Psp5II RGGWCCY 1 cut(s) 767
Psp6I CCWGG 4 cut(s) 126, 1063, 1647, 2917
PspCI CACGTG 1 cut(s) 2312
PspGI CCWGG 4 cut(s) 126, 1063, 1647, 2917
PspN4I GGNNCC 7 cut(s) 412, 480, 553, 768, 2560, 2577, 3022
PspOMI GGGCCC 1 cut(s) 551
PspPI GGNCC 8 cut(s) 4, 479, 551, 552, 767, 1383, 1814, 3008
PspPPI RGGWCCY 1 cut(s) 767
PsrI GAACNNNNNNTAC 2 cut(s) 3013, 3045
PstI CTGCAG 2 cut(s) 1915, 1987
PsuI RGATCY 3 cut(s) 193, 915, 1755
PsyI GACNNNGTC 1 cut(s) 1539
PvuII CAGCTG 1 cut(s) 1196
RruI TCGCGA 2 cut(s) 2324, 2442
RseI CAYNNNNRTG 1 cut(s) 1730
SacI GAGCTC 2 cut(s) 257, 1998
SatI GCNGC 7 cut(s) 105, 274, 1914, 1983, 2049, 2070, 2150
Sau3AI GATC 7 cut(s) 193, 808, 915, 1755, 1977, 2758, 2914
Sau96I GGNCC 8 cut(s) 4, 479, 551, 552, 767, 1383, 1814, 3008
SchI GAGTC 4 cut(s) 718, 1221, 1530, 2749
ScrFI CCNGG 4 cut(s) 128, 1065, 1649, 2919
SduI GDGCHC 4 cut(s) 257, 555, 1998, 2317
SfaNI GCATC 6 cut(s) 1414, 1517, 1800, 2056, 2446, 2793
SfcI CTRYAG 3 cut(s) 1911, 1983, 2677
SinI GGWCC 4 cut(s) 479, 767, 1814, 3008
SmiMI CAYNNNNRTG 1 cut(s) 1730
SmlI CTYRAG 4 cut(s) 523, 1129, 1751, 2862
SmoI CTYRAG 4 cut(s) 523, 1129, 1751, 2862
SphI GCATGC 1 cut(s) 2878
SseBI AGGCCT 1 cut(s) 2851
SsiI CCGC 4 cut(s) 920, 1657, 2607, 2658
SspI AATATT 3 cut(s) 505, 1306, 2780
SspMI CTAG 6 cut(s) 641, 780, 2364, 2381, 2391, 2645
SstI GAGCTC 2 cut(s) 257, 1998
StuI AGGCCT 1 cut(s) 2851
StyD4I CCNGG 4 cut(s) 126, 1063, 1647, 2917
StyI CCWWGG 2 cut(s) 2380, 2766
TaaI ACNGT 4 cut(s) 1093, 1491, 2134, 2303
TaiI ACGT 2 cut(s) 303, 2314
TaqI TCGA 3 cut(s) 1479, 1635, 2179
TaqII GACCGA 1 cut(s) 2092
TatI WGTACW 5 cut(s) 1394, 1491, 1641, 2286, 2943
TfiI GAWTC 5 cut(s) 1083, 1481, 1688, 2417, 3041
TscAI CASTG 4 cut(s) 76, 348, 1098, 2148
TseI GCWGC 7 cut(s) 104, 273, 1913, 1982, 2048, 2069, 2149
TspGWI ACGGA 1 cut(s) 549
TspRI CASTG 4 cut(s) 76, 348, 1098, 2148
Tth111I GACNNNGTC 1 cut(s) 1539
VpaK11BI GGWCC 4 cut(s) 479, 767, 1814, 3008
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 1 cut(s) 201
XapI RAATTY 5 cut(s) 328, 1168, 2183, 2476, 2693
XceI RCATGY 2 cut(s) 1224, 2878
XcmI CCANNNNNNNNNTGG 2 cut(s) 442, 2569
XmaJI CCTAGG 1 cut(s) 2380
XmiI GTMKAC 2 cut(s) 2106, 2700
XspI CTAG 6 cut(s) 641, 780, 2364, 2381, 2391, 2645
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.