Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006050

Physical Location & Seq

Genomic Coordinates Reverse (-)

27404228 .. 27406362

Gene Structure 2135 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1224500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 963 bp

ATGGACTACTCAGCTGCAAATGATGATGATGCTTCTTTGGATCTTAATTCCATGCCTCTCAGACTTTTCGATGATACTCCGATGATCAAAAAAGAGGTGCATAGCAAAATATTGATCGACTTCGGGAGGCAGCTTTCACCTAAAGAAGAGAGTGGTGCTCTATTGGAAGAATTGCACCGAGTGAGTGCAGAGAACAAGAAGCTAACCGAAATGTTGACGGTGATGGGAGAGAACTACAATGCTTTGAGAAGCCAACTGTTGGAGTACATGAGCAAGAACCCTGAGAAGGAGCTTAGCCCAATTTCAAAGAAAAGAAAGTCCGAAAGCAGCAACAACAACACAAACAGTAATAATATCATGAATGGAGCAGTGAATGGAAACTCGGAGAGCAGTTCCAGTGATGGAGAGTCCTACAAGAAACCAAGAGAAGAGATCGTCAAGGCTAAGATTTCAAGGGCTTGTGTCCAGACCGAAGCATCTGATACAAGCTTGGTTGTGAAAGATGGATATCAATGGAGGAAATATGGCCAAAAAGTTACTAGGGATAATCCTTGTCCTAGAGCTTACTTCAAATGCTCTTTTGCTCCAAGCTGCCCTGTCAAAAAGAAGGTTCAAAGAAGTGTTGAGGACCAATCTATTTTGGTGGCAACTTATGAAGGTGAACACAACCACCCCAACCCTTCCCAAATTGAAGCAACATCGGACTCAAACCACTGCGTGGCAATAGGATCCGTCCCTTGCTCAACCTCCCTTGGCTCAACTGGACCTACAATTACTCTTGACTTGACCAAGTCCAAGTCCAGTACTGCTGATGCCAAGAGCACAAAAATAAGAACTGAAACACCCGAAGTTCGCAAATTTTTGGTGGAGCAGATGGCTTCTTCCTTGACAAAGGATCCCGATTTCACGAAAGCACTGGCAGCAGCCATATCAGGAAGAATACTTCAACACAATACTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0002237 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0009890 GO:0009892 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046677 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

321

Amino Acids

35.31

Weight (kDa)

7.59

Isoelectric Point (pI)

44.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	167 - 225	1.6e-24	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006050)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80840 AT2G25000 AT2G25000 AT2G25000 AT2G25000 AT3G32090 AT4G31800 AT4G31800 AT4G31800
fragaria_vesca	FvH4_6g28650 FvH4_6g28650 FvH4_6g28650
malus_domestica	MD09G1224500.v1.1 MD17G1223100.v1.1
prunus_persica	Prupe.3G098100_v2.0.a1
pyrus_communis	pycom09g14410 pycom17g22700
rosa_chinensis	RchiOBHm_Chr2g0133001
rosa_laevigata	RLG00000019302
rosa_multiflora	Rmu_sc0000341.1_g000003
rosa_roxburghii	Rroxscaffold_2G00111570
rosa_rugosa	Rorug02G0309200
rosa_samantha	Rh2AG361500 Rh2BG367200 Rh2CG344700 Rh2DG384200
rosa_wichuraiana	Rw2G029400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	259, 718
AclWI	GGATC	5 cut(s)	48, 723, 736, 890, 903
AcoI	YGGCCR	1 cut(s)	526
AcsI	RAATTY	1 cut(s)	857
AdeI	CACNNNGTG	2 cut(s)	181, 718
AfaI	GTAC	2 cut(s)	266, 805
AfiI	CCNNNNNNNGG	3 cut(s)	259, 286, 718
AgsI	TTSAA	6 cut(s)	306, 453, 571, 614, 692, 947
AluBI	AGCT	7 cut(s)	14, 133, 202, 292, 489, 563, 591
AluI	AGCT	7 cut(s)	14, 133, 202, 292, 489, 563, 591
Alw21I	GWGCWC	2 cut(s)	160, 824
AlwI	GGATC	5 cut(s)	48, 723, 736, 890, 903
AoxI	GGCC	1 cut(s)	526
ApeKI	GCWGC	6 cut(s)	14, 130, 327, 591, 920, 923
ApoI	RAATTY	1 cut(s)	857
AspS9I	GGNCC	2 cut(s)	628, 764
AsuHPI	GGTGA	3 cut(s)	129, 232, 671
AvaII	GGWCC	2 cut(s)	628, 764
BalI	TGGCCA	1 cut(s)	528
BamHI	GGATCC	2 cut(s)	728, 895
Bbv12I	GWGCWC	2 cut(s)	160, 824
BbvI	GCAGC	5 cut(s)	142, 339, 578, 932, 935
BccI	CCATC	4 cut(s)	217, 395, 497, 868
BclI	TGATCA	1 cut(s)	84
BfaI	CTAG	2 cut(s)	540, 558
BisI	GCNGC	6 cut(s)	15, 131, 328, 592, 921, 924
BlpI	GCTNAGC	1 cut(s)	293
BlsI	GCNGC	6 cut(s)	16, 132, 329, 593, 922, 925
BmcAI	AGTACT	1 cut(s)	805
Bme18I	GGWCC	2 cut(s)	628, 764
BmgT120I	GGNCC	2 cut(s)	628, 764
BmiI	GGNNCC	2 cut(s)	730, 897
BmsI	GCATC	3 cut(s)	19, 485, 802
BplI	GAGNNNNNCTC	2 cut(s)	142, 174
Bpu1102I	GCTNAGC	1 cut(s)	293
BsaBI	GATNNNNATC	1 cut(s)	507
BsaJI	CCNNGG	1 cut(s)	751
Bsc4I	CCNNNNNNNGG	3 cut(s)	259, 286, 718
Bse1I	ACTGG	4 cut(s)	396, 766, 801, 921
Bse8I	GATNNNNATC	1 cut(s)	507
BseDI	CCNNGG	1 cut(s)	751
BseJI	GATNNNNATC	1 cut(s)	507
BseLI	CCNNNNNNNGG	3 cut(s)	259, 286, 718
BseMII	CTCAG	3 cut(s)	24, 73, 273
BseNI	ACTGG	4 cut(s)	396, 766, 801, 921
BseXI	GCAGC	5 cut(s)	142, 339, 578, 932, 935
BsgI	GTGCAG	1 cut(s)	207
BshFI	GGCC	1 cut(s)	528
BsiHKAI	GWGCWC	2 cut(s)	160, 824
BslFI	GGGAC	1 cut(s)	719
BslI	CCNNNNNNNGG	3 cut(s)	259, 286, 718
BsmFI	GGGAC	1 cut(s)	719
BsnI	GGCC	1 cut(s)	528
Bsp1286I	GDGCHC	2 cut(s)	160, 824
Bsp143I	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
Bsp1720I	GCTNAGC	1 cut(s)	293
BspANI	GGCC	1 cut(s)	528
BspCNI	CTCAG	3 cut(s)	23, 72, 274
BspHI	TCATGA	1 cut(s)	357
BspLI	GGNNCC	2 cut(s)	730, 897
BspPI	GGATC	5 cut(s)	48, 723, 736, 890, 903
BsrI	ACTGG	4 cut(s)	396, 766, 801, 921
BssECI	CCNNGG	1 cut(s)	751
BssMI	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
BssT1I	CCWWGG	1 cut(s)	751
Bst4CI	ACNGT	3 cut(s)	220, 258, 347
Bst6I	CTCTTC	2 cut(s)	141, 423
BstDEI	CTNAG	5 cut(s)	10, 59, 282, 293, 444
BstKTI	GATC	6 cut(s)	43, 87, 117, 435, 731, 898
BstMBI	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
BstMWI	GCNNNNNNNGC	1 cut(s)	920
BstV1I	GCAGC	5 cut(s)	142, 339, 578, 932, 935
BstX2I	RGATCY	3 cut(s)	40, 728, 895
BstYI	RGATCY	3 cut(s)	40, 728, 895
BsuRI	GGCC	1 cut(s)	528
BtsI	GCAGTG	2 cut(s)	375, 712
BtsIMutI	CAGTG	4 cut(s)	375, 403, 712, 914
CciI	TCATGA	1 cut(s)	357
Cfr13I	GGNCC	2 cut(s)	628, 764
Csp6I	GTAC	2 cut(s)	265, 804
CviAII	CATG	3 cut(s)	52, 268, 358
CviQI	GTAC	2 cut(s)	265, 804
DdeI	CTNAG	5 cut(s)	10, 59, 282, 293, 444
DpnI	GATC	6 cut(s)	42, 86, 116, 434, 730, 897
DpnII	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
DraIII	CACNNNGTG	2 cut(s)	181, 718
EaeI	YGGCCR	1 cut(s)	526
Eam1104I	CTCTTC	2 cut(s)	141, 423
EarI	CTCTTC	2 cut(s)	141, 423
Eco130I	CCWWGG	1 cut(s)	751
Eco32I	GATATC	1 cut(s)	509
Eco47I	GGWCC	2 cut(s)	628, 764
EcoRV	GATATC	1 cut(s)	509
EcoT14I	CCWWGG	1 cut(s)	751
ErhI	CCWWGG	1 cut(s)	751
FaeI	CATG	3 cut(s)	55, 271, 361
FaiI	YATR	7 cut(s)	53, 102, 269, 359, 525, 654, 929
FaqI	GGGAC	1 cut(s)	719
FatI	CATG	3 cut(s)	51, 267, 357
FbaI	TGATCA	1 cut(s)	84
Fnu4HI	GCNGC	6 cut(s)	15, 131, 328, 592, 921, 924
Fsp4HI	GCNGC	6 cut(s)	15, 131, 328, 592, 921, 924
FspBI	CTAG	2 cut(s)	540, 558
GluI	GCNGC	6 cut(s)	15, 131, 328, 592, 921, 924
HaeIII	GGCC	1 cut(s)	528
Hin1II	CATG	3 cut(s)	55, 271, 361
HincII	GTYRAC	1 cut(s)	216
HindII	GTYRAC	1 cut(s)	216
HindIII	AAGCTT	1 cut(s)	487
HinfI	GANTC	2 cut(s)	407, 704
HphI	GGTGA	3 cut(s)	129, 232, 671
Hpy166II	GTNNAC	2 cut(s)	216, 662
Hpy188I	TCNGA	6 cut(s)	62, 81, 322, 385, 481, 703
Hpy188III	TCNNGA	7 cut(s)	124, 358, 466, 779, 899, 907, 933
Hpy8I	GTNNAC	2 cut(s)	216, 662
HpyAV	CCTTC	4 cut(s)	280, 601, 650, 690
HpyCH4III	ACNGT	3 cut(s)	220, 258, 347
HpyCH4V	TGCA	4 cut(s)	17, 100, 175, 188
HpyF10VI	GCNNNNNNNGC	1 cut(s)	920
HpyF3I	CTNAG	5 cut(s)	10, 59, 282, 293, 444
Hsp92II	CATG	3 cut(s)	55, 271, 361
Ksp22I	TGATCA	1 cut(s)	84
Kzo9I	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
LmnI	GCTCC	4 cut(s)	289, 365, 589, 868
LpnPI	CCDG	8 cut(s)	294, 409, 479, 609, 747, 814, 902, 918
Lsp1109I	GCAGC	5 cut(s)	142, 339, 578, 932, 935
LweI	GCATC	3 cut(s)	19, 485, 802
MaeI	CTAG	2 cut(s)	540, 558
MaeIII	GTNAC	1 cut(s)	535
MalI	GATC	6 cut(s)	42, 86, 116, 434, 730, 897
MboI	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
MboII	GAAGA	5 cut(s)	158, 179, 440, 873, 948
MflI	RGATCY	3 cut(s)	40, 728, 895
MhlI	GDGCHC	2 cut(s)	160, 824
MlsI	TGGCCA	1 cut(s)	528
MluCI	AATT	6 cut(s)	46, 170, 300, 687, 771, 857
MluNI	TGGCCA	1 cut(s)	528
MlyI	GAGTC	2 cut(s)	416, 698
MmeI	TCCRAC	1 cut(s)	240
MnlI	CCTC	6 cut(s)	66, 88, 120, 510, 619, 757
Mox20I	TGGCCA	1 cut(s)	528
MscI	TGGCCA	1 cut(s)	528
MseI	TTAA	1 cut(s)	45
Msp20I	TGGCCA	1 cut(s)	528
MspA1I	CMGCKG	1 cut(s)	14
MwoI	GCNNNNNNNGC	1 cut(s)	920
NdeII	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
NlaIII	CATG	3 cut(s)	55, 271, 361
NlaIV	GGNNCC	2 cut(s)	730, 897
PagI	TCATGA	1 cut(s)	357
PflFI	GACNNNGTC	1 cut(s)	790
PflMI	CCANNNNNTGG	2 cut(s)	259, 718
PkrI	GCNGC	6 cut(s)	16, 132, 329, 593, 922, 925
PleI	GAGTC	2 cut(s)	415, 698
PpsI	GAGTC	2 cut(s)	415, 698
PspN4I	GGNNCC	2 cut(s)	730, 897
PspPI	GGNCC	2 cut(s)	628, 764
PsuI	RGATCY	3 cut(s)	40, 728, 895
PsyI	GACNNNGTC	1 cut(s)	790
PvuII	CAGCTG	1 cut(s)	14
RsaI	GTAC	2 cut(s)	266, 805
RsaNI	GTAC	2 cut(s)	265, 804
SaqAI	TTAA	1 cut(s)	45
SatI	GCNGC	6 cut(s)	15, 131, 328, 592, 921, 924
Sau3AI	GATC	6 cut(s)	40, 84, 114, 432, 728, 895
Sau96I	GGNCC	2 cut(s)	628, 764
ScaI	AGTACT	1 cut(s)	805
SchI	GAGTC	2 cut(s)	416, 698
SduI	GDGCHC	2 cut(s)	160, 824
SfaNI	GCATC	3 cut(s)	19, 485, 802
SinI	GGWCC	2 cut(s)	628, 764
Sse9I	AATT	6 cut(s)	46, 170, 300, 687, 771, 857
SspI	AATATT	1 cut(s)	111
SspMI	CTAG	2 cut(s)	540, 558
StyI	CCWWGG	1 cut(s)	751
TaaI	ACNGT	3 cut(s)	220, 258, 347
TaqI	TCGA	2 cut(s)	69, 117
TaqII	GACCGA	1 cut(s)	485
TasI	AATT	6 cut(s)	46, 170, 300, 687, 771, 857
TatI	WGTACW	2 cut(s)	264, 803
Tru1I	TTAA	1 cut(s)	45
Tru9I	TTAA	1 cut(s)	45
TscAI	CASTG	4 cut(s)	375, 403, 719, 921
TseI	GCWGC	6 cut(s)	14, 130, 327, 591, 920, 923
TspDTI	ATGAA	2 cut(s)	374, 669
TspGWI	ACGGA	1 cut(s)	721
TspRI	CASTG	4 cut(s)	375, 403, 719, 921
Tth111I	GACNNNGTC	1 cut(s)	790
Van91I	CCANNNNNTGG	2 cut(s)	259, 718
VpaK11BI	GGWCC	2 cut(s)	628, 764
XapI	RAATTY	1 cut(s)	857
XspI	CTAG	2 cut(s)	540, 558
ZrmI	AGTACT	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.