Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0006050

Physical Location & Seq

Genomic Coordinates Reverse (-)

35397098 .. 35428383

Gene Structure 31286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0309200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4857 bp

ATGGCGGCGTTGCAGTCACTGGCTCCTGTTCCTCAGCTGCTTCACTCCTCCAATGGCCGCTCCCCGATGAAAGGACTCTTTCTCGTCGATTTTGTCGGCTACTGCAAATCAAAGAGGACTCGCCGTAAATTCAACGGAGCATCCCAGCCTCTACGGACAAGCTTCCCTCAATTCGTTTCCAAAGCCGTGCTCGACCTTGGACCCTCCTCTACCGATCACTCAACTCCTCCTCCTCCTCCTCCTACTACGACTTCCAAACCTCAGGTTGCGGACCTGAAAGATATAATATCAGAAAGAGGAGCTTGCGGAGTTGGATTCATTGCCAATCTGGACAACAAAGCATCTCATGGCATTGTTAAGGATGCTCTTACAGCTCTTGGCTGCATGGAACATCGTGGAGGCTGTGGAGCAGATAATGACTCTGGTGATGGTTCAGGATTGATGAGTTCAATCCCATGGGATCTATTTAACAATTGGGCTGACAAGCAAGGGATTGCATCGTTTGACAAGTTACACACTGGTGTTGGAATGATTTTCCTCCCCAAAGAGGACAACCTTATGAAAGAAGCCAAAAAAGCTATTGTGAATATTTTTAAACAAGAGGGTCTTGAAGTGCTTGGGTGGAGACCTGTTCCAGTGAATACAGCTATAGTTGGTTTCTATGCAAAAGAAACAATGCCCAGCATACAACAGGTATTCGTCAAAGTTGTTAAAGAGGAAAAAGTTGACAACATTGAGCGAGAACTGTACATATGCCGGAAGTTAATCGAAAGAGCAGCAAGCTCAGAAAGTTGGGGCAGTGATCTTTATTTCTGTTCTTTGTCCAATCAAACAATAGTTTACAAAGGAATGCTACGCTCAGAAGTTCTTGGTTTATTTTATTCTGACCTCCAAAGTGACCTTTATAAATCCGCTTTTGCCATCTATCATCGAAGGTATAGCACAAATACCACTCCTAGATGGCCCCTTGCACAACCAATGAGGTTACTTGGTCACAACGGAGAGATCAACACCATACAGGGGAACTTGCACTGGATGCAATCACGTGAAGCCTCACTGAAGTCACCTGTATGGCATGATCGTGAAAATGAACTTCGTCCATATGGCAATCCAAAGGGTTCAGACTCTGCGAATCTTGACAGTGCGGCTGAATTCTTACTAAGAAGTGGCCGGACTGCTGAGGAGGCTCTGATGATTCTTGTCCCTGAGGGATACAAAAATCATCCAACTCTGATGATTAACTATCCAGAGGTGGTTGATTTTTATGATTATTACAAGGGTCAGATGGAGGCTTGGGATGGACCTGCTTTATTGTTATTCAGTGATGGAAAAACAGTTGGGGCATGTCTTGATCGTAATGGACTTCGGCCTGCTAGATATTGGCGGACATCTGATAACGTTGTTTATGTCGCATCTGAGGTTGGGGTTCTTCCAGTGGATGATTCAAAAGTTACAATGAAAGGTCGTCTAGGTCCTGGAATGATGATATCTGTTGACTTACTAAGTGGTCAGGTTTATGAGAACACAGAAGTTAAGAAGCGAGTGGCTTTGTCAAATCCATATGGAAAGTGGGTGCAGGAAAACATGCGGACCTTAAAGGCTGTGAACTTCCTTTCATCCACAATCGCGGACAATGATGCAATACTGAGACGGCAACAGGCATTTGGTTACTCGAGTGAGGATGTCCAAATGGTTATCGAGACAATGGCTTCACAGGGGAAGGAGCCTACATTTTGCATGGGTGATGATATTCCATTGGCGATTTTATCTCAGAGACCACATATGCTATATGACTATTTCAAGCAACGGTTTGCGCAGGTTACAAATCCAGCCATTGATCCTCTAAGAGAAGGGTTAGTTATGTCTCTTGAAGTCAACATTGGAGAGCGACGAAATATTTTGGAGGTTGGACCTGAAAATGCATCGCAGGTTATCTTGTCAAGTCCTGTGCTCAATGAAGGAGAACTTGAGTCATTGCTTAAGGATGCTCAGCTAAAACCCCAAGTTTTACCTACATTTTTTGATATTCATAAAGGGGTCGATGGTTCCTTGGAAAAAACGCTGTATAAGCTTTGTGAAGCTGCTGATGATGCTGTTCAAAATGGTTGCCAATTGCTTGTTCTGTCTGACCGGTCTGATGAGTTGGAAGCAACTCATCCTGCAATTCCAATCCTTCTTGCTGTTGGTGCTGTTCATCAGCATCTTATTCAAAATGGGTTGAGAATGTCAGCTTCTATAATAGTGGATACTGCTCAGTGCTTTAGCACTCATCAATTTGCCTGTTTGATTGGATATGGTGCAAGTGCTGTGTGTCCATACTTGGCATTGGAGACATGTCGGCAGTGGCGTCTGAGTAATAAAACAGTAAATCTAATGCGGAATGGGAAGATGCCTACTGTAACGATTGAACAGGCTCAAAAGAACTTCTGCAAGGCTGTTACGGCTGGTCTTCTTAAAATTCTTTCCAAAATGGGGATCTCGTTGCTTTCAAGTTATTGCGGTGCACAGATTTTTGAGATTTATGGATTGGGGAAGGAGGTTGTTGATCTTGCATTTTGTGGGAGTATATCAAGTATTGGTGGATTAACTTTTGATGAGCTGGCGAGGGAGACTTTGTCCTTCTGGGTGAAGGCTTTCTCTGAGGATACAGCTAAACGACTGGAAAATTTTGGATTTATACAATTCAGACCAGGAGGGGAGTATCATGGAAACAACCCAGAGATGTCGAAATTGCTACACAAAGCTGTTCGACAGAAGAATGAAAGTGCCTTTTCTGTTTATCAGCAGCATTTAGCTAACCGGCCCGTTAATGTTCTTCGCGATCTCATTGAGTTCAAAAGCGATCGTGCTCCAATCCCTGTGGGAAAGGTTGAACCTGCTGTATCCATTGTTCAACGATTCTGCACTGGTGGGATGTCACTTGGAGCTATTTCCAGAGAAACTCATGAGGCTATAGCCATTGCAATGAATAGAATAGGTGGAAAGTCCAATTCTGGAGAAGGTGGTGAGGATCCTATTCGCTGGAAACCACTTGCAGATGTTGTTGATGGGTATTCACCGACCCTTCCACATCTAAAAGGTCTTCAAAATGGAGATACTGCTACAAGTGCTATCAAGCAGGTTGCTTCAGGACGTTTTGGAGTCACTCCAACCTTCTTGGTCAATGCCGATCAGCTGGAAATCAAAATTGCACAAGGGGCGAAGCCTGGTGAAGGTGGCCAACTGCCTGGTAAAAAAGTTAGTGCATACATTGCAAGGTTGAGAAACTCGAAACCTGGGGTTCCACTTATATCTCCACCCCCACACCATGACATTTATTCTATAGAGGATCTTGCCCAATTGATCTATGACCTTCATCAGGTGAACCCTAAAGCTAAGGTGTCAGTGAAACTTGTGGCTGAAGCTGGAATTGGCACTGTTGCTTCTGGGGTCGCAAAGGGTAATGCTGATATCATACAGATATCAGGGCATGATGGTGGAACTGGGGCCAGTCCTATAAGTTCCATTAAGCATGCTGGTGGGCCCTGGGAACTAGGACTCACAGAAACACACCAGACACTCATAGAAAATGGACTTAGAGAACGAGTCATTCTTAGAGTTGATGGTGGCTTTAAAAGCGGAGTTGATGTTCTAATGGCTGCAACTATGGGTGCTGATGAGTACGGGTTTGGGTCTGTGGCAATGATTGCTACAGGTTGTGTTATGGCCCGCATTTGCCATACGAATAATTGTCCTGTTGGTGTAGCCAGCCAGAGAGAGGAACTGCGTGCTCGATTTCCTGGCGTGCCTGGTGATCTAGTCAACTTTTTTTTGTATGTTGCCGAAGAGGTAAGAGGCATGTTGGCACAGTTGGGATATGAGAAGTTAGACGATATAATTGGACGGACAGATTTGTTTAGACCACGAGATATATCTCTAATGAAAACACAGCATCTTGATCTCGGTTATATTCTATCAAATGTCGGGTTGCCAAAATGGAGCAGTACCATGATCAGGAATCAGGATGTTCATACTAACGGTCCTGTTCTGGATGATATTTTACTTGGAGATCCAGAGATATCAGATGCAATTGAGAATGAAAAAGTGGTTCAGAAGACTGTAAAGATATACAATGTGGATCGTGCTGTTTGTGGGCGTATAGCAGGGGTGGTTGCCAAAAAGTACGGTGACACTGGTTTTGCTGGGCAGCTGAACATAACCTTTACAGGGAGCGCTGGGCAATCATTTGGTTGCTTCTTGACTCCTGGGATGAATATTCGACTTGTTGGAGAGGCAAATGATTATGTGGGAAAGAGTATTTCGGGGGGAGAATTGGTTGTCACTCCGGCAGAGAATACGGGATTTTGCCCTGAGGATGCAACTATTGTAGGGAATACCTGCTTGTATGGTGCAACTGGGGGTCAAATCTTTGTTAGAGGGAAAGCCGGAGAGCGATTTGCTGTAAGAAACTCTCTTGCGCAAGCAGTGGTTGAAGGCGCTGGAGACCACTGTTGTGAATACATGACTGGTGGCTGTGTAGTTGTACTTGGAAAAGTGGGTAGAAATGTTGCTGCTGGAATGACTGGAGGTCTGGCATACTTTCTTGATGAGGACGATTCTTTCATCCCCAAGGTAAATAGGGAGATTGTGAAGATCCAAAGAGTCAATGCTCCTGTGGGGCAGATGCAGCTAAAGAGCCTTATTGAAGCCCATGTTGAAAAAACTGGAAGTGGCAAGGGCTCTGTTATTCTGAAAGAGTGGGACAAGTACTTGCCACTGTTCTGGCAGCTGGTTCCCCCTAGTGAAGAAGACACTCCAGAGGCGTGTGCAGACTATGTGAGAACAGCTGCTGGTGAAGTGACTTTGCAGTCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

1618

Amino Acids

176.07

Weight (kDa)

6.2

Isoelectric Point (pI)

35.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	102 - 524	3.8e-176	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	550 - 843	3e-107	Glutamate synthase central domain
Glu_synthase	PF01645	903 - 1286	3e-155	Conserved region in glutamate synthase
GXGXG	PF01493	1368 - 1551	2.1e-78	GXGXG motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006050)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80840 AT2G25000 AT2G25000 AT2G25000 AT2G25000 AT3G32090 AT4G31800 AT4G31800 AT4G31800
fragaria_vesca	FvH4_6g28650 FvH4_6g28650 FvH4_6g28650
malus_domestica	MD09G1224500.v1.1 MD17G1223100.v1.1
prunus_persica	Prupe.3G098100_v2.0.a1
pyrus_communis	pycom09g14410 pycom17g22700
rosa_chinensis	RchiOBHm_Chr2g0133001
rosa_laevigata	RLG00000019302
rosa_multiflora	Rmu_sc0000341.1_g000003
rosa_roxburghii	Rroxscaffold_2G00111570
rosa_rugosa	Rorug02G0309200
rosa_samantha	Rh2AG361500 Rh2BG367200 Rh2CG344700 Rh2DG384200
rosa_wichuraiana	Rw2G029400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	906
AasI	GACNNNNNNGTC	1 cut(s)	4846
Acc16I	TGCGCA	2 cut(s)	1815, 4458
Acc36I	ACCTGC	6 cut(s)	1312, 1807, 1918, 2884, 3109, 4385
AccBSI	CCGCTC	1 cut(s)	60
AccII	CGCG	2 cut(s)	1628, 2820
AclI	AACGTT	1 cut(s)	1398
AclWI	GGATC	9 cut(s)	468, 1832, 2483, 3005, 3018, 3336, 4043, 4125, 4627
AcoI	YGGCCR	3 cut(s)	55, 1168, 3217
AcsI	RAATTY	4 cut(s)	128, 1151, 2457, 2665
AcuI	CTGAAG	3 cut(s)	1079, 3111, 3420
AcvI	CACGTG	1 cut(s)	1046
AcyI	GRCGYC	1 cut(s)	2347
AfaI	GTAC	6 cut(s)	749, 3662, 3985, 4163, 4524, 4748
AfeI	AGCGCT	1 cut(s)	4213
AfiI	CCNNNNNNNGG	9 cut(s)	71, 547, 1020, 2472, 3212, 3358, 3757, 3993, 4206
AflII	CTTAAG	1 cut(s)	1979
AflIII	ACRYGT	1 cut(s)	2333
AgeI	ACCGGT	1 cut(s)	2130
AhdI	GACNNNNNGTC	1 cut(s)	4566
AjnI	CCWGG	9 cut(s)	1474, 2689, 3205, 3226, 3274, 3524, 3778, 3787, 4243
AjuI	GAANNNNNNNTTGG	6 cut(s)	1863, 1895, 2103, 2135, 3393, 3425
AleI	CACNNNNGTG	2 cut(s)	519, 4491
AloI	GAACNNNNNNTCC	2 cut(s)	3612, 3644
Alw21I	GWGCWC	5 cut(s)	192, 1953, 2506, 2851, 3772
Alw26I	GTCTC	8 cut(s)	619, 1642, 1694, 1768, 1869, 2324, 2603, 4476
Alw44I	GTGCAC	1 cut(s)	2502
AlwI	GGATC	9 cut(s)	468, 1832, 2483, 3005, 3018, 3336, 4043, 4125, 4627
AlwNI	CAGNNNCTG	3 cut(s)	19, 1127, 4829
Ama87I	CYCGRG	1 cut(s)	1672
Aor51HI	AGCGCT	1 cut(s)	4213
AoxI	GGCC	9 cut(s)	55, 962, 1168, 1367, 2801, 3217, 3486, 3521, 3705
ApaI	GGGCCC	1 cut(s)	3525
ApaLI	GTGCAC	1 cut(s)	2502
ApoI	RAATTY	4 cut(s)	128, 1151, 2457, 2665
ArsI	GACNNNNNNTTYG	2 cut(s)	4160, 4192
AsiGI	ACCGGT	1 cut(s)	2130
Asp700I	GAANNNNTTC	1 cut(s)	2633
AspLEI	GCGC	4 cut(s)	1816, 4214, 4459, 4478
AvaI	CYCGRG	1 cut(s)	1672
AvaII	GGWCC	7 cut(s)	200, 271, 1301, 1472, 1590, 1910, 4019
AxyI	CCTNAGG	3 cut(s)	261, 1206, 4350
BaeGI	GKGCMC	2 cut(s)	2506, 3525
BalI	TGGCCA	1 cut(s)	3219
BamHI	GGATCC	1 cut(s)	3010
BanII	GRGCYC	2 cut(s)	3525, 4721
BauI	CACGAG	1 cut(s)	3903
BbrPI	CACGTG	1 cut(s)	1046
BbsI	GAAGAC	4 cut(s)	2441, 3074, 4100, 4794
Bbv12I	GWGCWC	5 cut(s)	192, 1953, 2506, 2851, 3772
BbvCI	CCTCAGC	2 cut(s)	33, 1179
BceAI	ACGGC	4 cut(s)	108, 170, 1667, 2457
BciT130I	CCWGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
BciVI	GTATCC	4 cut(s)	1205, 2239, 2638, 2893
BclI	TGATCA	1 cut(s)	3990
BcoDI	GTCTC	8 cut(s)	619, 1642, 1694, 1768, 1869, 2324, 2603, 4476
BfaI	CTAG	6 cut(s)	957, 1374, 1469, 3533, 3797, 4779
BfmI	CTRYAG	4 cut(s)	648, 2952, 3321, 3690
BfoI	RGCGCY	2 cut(s)	4215, 4479
BfrI	CTTAAG	1 cut(s)	1979
BfuAI	ACCTGC	6 cut(s)	1312, 1807, 1918, 2884, 3109, 4385
BfuI	GTATCC	4 cut(s)	1205, 2239, 2638, 2893
BlpI	GCTNAGC	1 cut(s)	1989
BmcAI	AGTACT	1 cut(s)	4748
Bme1390I	CCNGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
Bme18I	GGWCC	7 cut(s)	200, 271, 1301, 1472, 1590, 1910, 4019
BmeRI	GACNNNNNGTC	1 cut(s)	4566
BmeT110I	CYCGRG	1 cut(s)	1672
BmrFI	CCNGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
BmrI	ACTGGG	2 cut(s)	3492, 4404
BmuI	ACTGGG	2 cut(s)	3492, 4404
BpiI	GAAGAC	4 cut(s)	2441, 3074, 4100, 4794
BpmI	CTGGAG	4 cut(s)	3015, 4500, 4584, 4779
Bpu10I	CCTNAGC	3 cut(s)	33, 1179, 3375
Bpu1102I	GCTNAGC	1 cut(s)	1989
BpuEI	CTTGAG	1 cut(s)	1988
BsaAI	YACGTR	1 cut(s)	1046
BsaHI	GRCGYC	1 cut(s)	2347
BsaI	GGTCTC	3 cut(s)	619, 1768, 4476
BsaJI	CCNNGG	8 cut(s)	196, 455, 2049, 3275, 3524, 3525, 4244, 4608
BsaWI	WCCGGW	1 cut(s)	2130
BsaXI	ACNNNNNCTCC	8 cut(s)	214, 244, 2600, 2630, 3612, 3642, 4299, 4329
Bsc4I	CCNNNNNNNGG	9 cut(s)	71, 547, 1020, 2472, 3212, 3358, 3757, 3993, 4206
Bse118I	RCCGGY	2 cut(s)	2130, 2799
Bse21I	CCTNAGG	3 cut(s)	261, 1206, 4350
Bse3DI	GCAATG	6 cut(s)	318, 1973, 2958, 2970, 3249, 3687
BseBI	CCWGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
BseDI	CCNNGG	8 cut(s)	196, 455, 2049, 3275, 3524, 3525, 4244, 4608
BseLI	CCNNNNNNNGG	9 cut(s)	71, 547, 1020, 2472, 3212, 3358, 3757, 3993, 4206
BseMI	GCAATG	6 cut(s)	318, 1973, 2958, 2970, 3249, 3687
BseRI	GAGGAG	9 cut(s)	37, 196, 216, 219, 222, 225, 228, 312, 1196
BseSI	GKGCMC	2 cut(s)	2506, 3525
BseYI	CCCAGC	4 cut(s)	144, 680, 4181, 4214
BsgI	GTGCAG	3 cut(s)	1595, 2887, 4827
Bsh1236I	CGCG	2 cut(s)	1628, 2820
Bsh1285I	CGRYCG	1 cut(s)	2845
BshFI	GGCC	9 cut(s)	57, 964, 1170, 1369, 2803, 3219, 3488, 3523, 3707
BshTI	ACCGGT	1 cut(s)	2130
BsiEI	CGRYCG	1 cut(s)	2845
BsiHKAI	GWGCWC	5 cut(s)	192, 1953, 2506, 2851, 3772
BsiHKCI	CYCGRG	1 cut(s)	1672
BsiSI	CCGG	6 cut(s)	757, 1171, 2131, 2800, 4325, 4425
BslFI	GGGAC	2 cut(s)	1187, 4754
BslI	CCNNNNNNNGG	9 cut(s)	71, 547, 1020, 2472, 3212, 3358, 3757, 3993, 4206
BsmAI	GTCTC	8 cut(s)	619, 1642, 1694, 1768, 1869, 2324, 2603, 4476
BsmBI	CGTCTC	1 cut(s)	1642
BsmFI	GGGAC	2 cut(s)	1187, 4754
BsmI	GAATGC	1 cut(s)	855
BsnI	GGCC	9 cut(s)	57, 964, 1170, 1369, 2803, 3219, 3488, 3523, 3707
Bso31I	GGTCTC	3 cut(s)	619, 1768, 4476
BsoBI	CYCGRG	1 cut(s)	1672
Bsp120I	GGGCCC	1 cut(s)	3521
Bsp1286I	GDGCHC	7 cut(s)	192, 1953, 2506, 2851, 3525, 3772, 4721
Bsp1407I	TGTACA	1 cut(s)	747
Bsp1720I	GCTNAGC	1 cut(s)	1989
Bsp19I	CCATGG	1 cut(s)	455
Bsp68I	TCGCGA	1 cut(s)	2820
BspANI	GGCC	9 cut(s)	57, 964, 1170, 1369, 2803, 3219, 3488, 3523, 3707
BspFNI	CGCG	2 cut(s)	1628, 2820
BspHI	TCATGA	1 cut(s)	2944
BspMI	ACCTGC	6 cut(s)	1312, 1807, 1918, 2884, 3109, 4385
BspPI	GGATC	9 cut(s)	468, 1832, 2483, 3005, 3018, 3336, 4043, 4125, 4627
BspTI	CTTAAG	1 cut(s)	1979
BspTNI	GGTCTC	3 cut(s)	619, 1768, 4476
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	6 cut(s)	318, 1973, 2958, 2970, 3249, 3687
BsrFI	RCCGGY	2 cut(s)	2130, 2799
BsrGI	TGTACA	1 cut(s)	747
BssAI	RCCGGY	2 cut(s)	2130, 2799
BssECI	CCNNGG	8 cut(s)	196, 455, 2049, 3275, 3524, 3525, 4244, 4608
BssNI	GRCGYC	1 cut(s)	2347
BssSI	CACGAG	1 cut(s)	3903
BssT1I	CCWWGG	4 cut(s)	196, 455, 2049, 4608
Bst2BI	CACGAG	1 cut(s)	3903
Bst2UI	CCWGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
Bst6I	CTCTTC	1 cut(s)	3819
BstACI	GRCGYC	1 cut(s)	2347
BstAFI	CTTAAG	1 cut(s)	1979
BstAPI	GCANNNNNTGC	3 cut(s)	1036, 3251, 3686
BstAUI	TGTACA	1 cut(s)	747
BstBAI	YACGTR	1 cut(s)	1046
BstDSI	CCRYGG	1 cut(s)	455
BstENI	CCTNNNNNAGG	2 cut(s)	3210, 3356
BstFNI	CGCG	2 cut(s)	1628, 2820
BstH2I	RGCGCY	2 cut(s)	4215, 4479
BstHHI	GCGC	4 cut(s)	1816, 4214, 4459, 4478
BstMAI	GTCTC	8 cut(s)	619, 1642, 1694, 1768, 1869, 2324, 2603, 4476
BstMCI	CGRYCG	1 cut(s)	2845
BstNI	CCWGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
BstNSI	RCATGY	5 cut(s)	1347, 1588, 2337, 3515, 3841
BstSCI	CCNGG	9 cut(s)	1474, 2689, 3205, 3226, 3274, 3524, 3778, 3787, 4243
BstSFI	CTRYAG	4 cut(s)	648, 2952, 3321, 3690
BstSLI	GKGCMC	2 cut(s)	2506, 3525
BstUI	CGCG	2 cut(s)	1628, 2820
BstV2I	GAAGAC	4 cut(s)	2441, 3074, 4100, 4794
BstX2I	RGATCY	6 cut(s)	460, 2475, 3010, 3328, 4048, 4632
BstXI	CCANNNNNNTGG	2 cut(s)	3227, 4761
BstYI	RGATCY	6 cut(s)	460, 2475, 3010, 3328, 4048, 4632
Bsu36I	CCTNAGG	3 cut(s)	261, 1206, 4350
BsuI	GTATCC	4 cut(s)	1205, 2239, 2638, 2893
BsuRI	GGCC	9 cut(s)	57, 964, 1170, 1369, 2803, 3219, 3488, 3523, 3707
BtgI	CCRYGG	1 cut(s)	455
BtgZI	GCGATG	1 cut(s)	1908
BtsI	GCAGTG	3 cut(s)	805, 2348, 4470
BtuMI	TCGCGA	1 cut(s)	2820
BveI	ACCTGC	6 cut(s)	1312, 1807, 1918, 2884, 3109, 4385
CaiI	CAGNNNCTG	3 cut(s)	19, 1127, 4829
CciI	TCATGA	1 cut(s)	2944
CfoI	GCGC	4 cut(s)	1816, 4214, 4459, 4478
Cfr10I	RCCGGY	2 cut(s)	2130, 2799
CseI	GACGC	1 cut(s)	2336
Csp6I	GTAC	6 cut(s)	748, 3661, 3984, 4162, 4523, 4747
CspAI	ACCGGT	1 cut(s)	2130
CspCI	CAANNNNNGTGG	2 cut(s)	2841, 2876
CviQI	GTAC	6 cut(s)	748, 3661, 3984, 4162, 4523, 4747
DraI	TTTAAA	2 cut(s)	595, 3613
DrdI	GACNNNNNNGTC	1 cut(s)	4846
DriI	GACNNNNNGTC	1 cut(s)	4566
DseDI	GACNNNNNNGTC	1 cut(s)	4846
EaeI	YGGCCR	3 cut(s)	55, 1168, 3217
Eam1104I	CTCTTC	1 cut(s)	3819
Eam1105I	GACNNNNNGTC	1 cut(s)	4566
EarI	CTCTTC	1 cut(s)	3819
EciI	GGCGGA	1 cut(s)	1399
Eco130I	CCWWGG	4 cut(s)	196, 455, 2049, 4608
Eco24I	GRGCYC	2 cut(s)	3525, 4721
Eco31I	GGTCTC	3 cut(s)	619, 1768, 4476
Eco32I	GATATC	4 cut(s)	1488, 3451, 3462, 4059
Eco47I	GGWCC	7 cut(s)	200, 271, 1301, 1472, 1590, 1910, 4019
Eco47III	AGCGCT	1 cut(s)	4213
Eco57I	CTGAAG	3 cut(s)	1079, 3111, 3420
Eco72I	CACGTG	1 cut(s)	1046
Eco81I	CCTNAGG	3 cut(s)	261, 1206, 4350
Eco88I	CYCGRG	1 cut(s)	1672
EcoNI	CCTNNNNNAGG	2 cut(s)	3210, 3356
EcoO109I	RGGNCCY	2 cut(s)	1472, 3522
EcoRI	GAATTC	1 cut(s)	1151
EcoRII	CCWGG	9 cut(s)	1474, 2689, 3205, 3226, 3274, 3524, 3778, 3787, 4243
EcoRV	GATATC	4 cut(s)	1488, 3451, 3462, 4059
EcoT14I	CCWWGG	4 cut(s)	196, 455, 2049, 4608
EcoT22I	ATGCAT	1 cut(s)	1924
EcoT38I	GRGCYC	2 cut(s)	3525, 4721
ErhI	CCWWGG	4 cut(s)	196, 455, 2049, 4608
Esp3I	CGTCTC	1 cut(s)	1642
FalI	AAGNNNNNCTT	6 cut(s)	286, 318, 591, 623, 1950, 1982
FaqI	GGGAC	2 cut(s)	1187, 4754
FauI	CCCGC	1 cut(s)	3716
FauNDI	CATATG	4 cut(s)	752, 1102, 1561, 1782
FbaI	TGATCA	1 cut(s)	3990
FriOI	GRGCYC	2 cut(s)	3525, 4721
FspBI	CTAG	6 cut(s)	957, 1374, 1469, 3533, 3797, 4779
FspI	TGCGCA	2 cut(s)	1815, 4458
GlaI	GCGC	4 cut(s)	1815, 4213, 4458, 4477
GsaI	CCCAGC	4 cut(s)	148, 684, 4185, 4218
GsuI	CTGGAG	4 cut(s)	3015, 4500, 4584, 4779
HaeII	RGCGCY	2 cut(s)	4215, 4479
HaeIII	GGCC	9 cut(s)	57, 964, 1170, 1369, 2803, 3219, 3488, 3523, 3707
HapII	CCGG	6 cut(s)	757, 1171, 2131, 2800, 4325, 4425
HgaI	GACGC	1 cut(s)	2336
HhaI	GCGC	4 cut(s)	1816, 4214, 4459, 4478
Hin1I	GRCGYC	1 cut(s)	2347
Hin6I	GCGC	4 cut(s)	1814, 4212, 4457, 4476
HinP1I	GCGC	4 cut(s)	1814, 4212, 4457, 4476
HincII	GTYRAC	4 cut(s)	727, 1495, 1876, 3802
HindII	GTYRAC	4 cut(s)	727, 1495, 1876, 3802
HindIII	AAGCTT	2 cut(s)	160, 2069
HpaII	CCGG	6 cut(s)	757, 1171, 2131, 2800, 4325, 4425
Hpy166II	GTNNAC	8 cut(s)	727, 841, 1495, 1606, 1876, 2504, 3364, 3802
Hpy8I	GTNNAC	8 cut(s)	727, 841, 1495, 1606, 1876, 2504, 3364, 3802
Hpy99I	CGWCG	2 cut(s)	89, 1893
HpyCH4IV	ACGT	3 cut(s)	1045, 1398, 3133
HpySE526I	ACGT	3 cut(s)	1045, 1398, 3133
Hsp92I	GRCGYC	1 cut(s)	2347
HspAI	GCGC	4 cut(s)	1814, 4212, 4457, 4476
Ksp22I	TGATCA	1 cut(s)	3990
MaeI	CTAG	6 cut(s)	957, 1374, 1469, 3533, 3797, 4779
MaeII	ACGT	3 cut(s)	1045, 1398, 3133
MbiI	CCGCTC	1 cut(s)	60
MfeI	CAATTG	4 cut(s)	472, 2111, 3338, 4068
MflI	RGATCY	6 cut(s)	460, 2475, 3010, 3328, 4048, 4632
MhlI	GDGCHC	7 cut(s)	192, 1953, 2506, 2851, 3525, 3772, 4721
MlsI	TGGCCA	1 cut(s)	3219
MluNI	TGGCCA	1 cut(s)	3219
MmeI	TCCRAC	7 cut(s)	292, 505, 1250, 1888, 2124, 3173, 4246
Mox20I	TGGCCA	1 cut(s)	3219
Mph1103I	ATGCAT	1 cut(s)	1924
MroXI	GAANNNNTTC	1 cut(s)	2633
MscI	TGGCCA	1 cut(s)	3219
MslI	CAYNNNNRTG	7 cut(s)	519, 1069, 1080, 2963, 3474, 3516, 4491
Msp20I	TGGCCA	1 cut(s)	3219
MspA1I	CMGCKG	5 cut(s)	37, 3175, 4189, 4768, 4826
MspCI	CTTAAG	1 cut(s)	1979
MspI	CCGG	6 cut(s)	757, 1171, 2131, 2800, 4325, 4425
MspR9I	CCNGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
MunI	CAATTG	4 cut(s)	472, 2111, 3338, 4068
Mva1269I	GAATGC	1 cut(s)	855
MvaI	CCWGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
MvnI	CGCG	2 cut(s)	1628, 2820
NcoI	CCATGG	1 cut(s)	455
NdeI	CATATG	4 cut(s)	752, 1102, 1561, 1782
NmuCI	GTSAC	9 cut(s)	15, 896, 992, 1062, 2916, 3142, 4166, 4318, 4837
NruI	TCGCGA	1 cut(s)	2820
NsbI	TGCGCA	2 cut(s)	1815, 4458
NsiI	ATGCAT	1 cut(s)	1924
NspI	RCATGY	5 cut(s)	1347, 1588, 2337, 3515, 3841
OliI	CACNNNNGTG	2 cut(s)	519, 4491
PaeI	GCATGC	1 cut(s)	3515
PaeR7I	CTCGAG	1 cut(s)	1672
PagI	TCATGA	1 cut(s)	2944
PasI	CCCWGGG	1 cut(s)	3525
PciI	ACATGT	1 cut(s)	2333
PcsI	WCGNNNNNNNCGW	1 cut(s)	2344
PctI	GAATGC	1 cut(s)	855
PdmI	GAANNNNTTC	1 cut(s)	2633
PfeI	GAWTC	7 cut(s)	315, 1132, 1195, 1442, 2898, 3997, 4595
PflFI	GACNNNGTC	1 cut(s)	2614
PfoI	TCCNGGA	1 cut(s)	1474
PinAI	ACCGGT	1 cut(s)	2130
Ple19I	CGATCG	1 cut(s)	2845
PmaCI	CACGTG	1 cut(s)	1046
PmlI	CACGTG	1 cut(s)	1046
Ppu21I	YACGTR	1 cut(s)	1046
PpuMI	RGGWCCY	1 cut(s)	1472
PscI	ACATGT	1 cut(s)	2333
PsiI	TTATAA	1 cut(s)	906
Psp1406I	AACGTT	1 cut(s)	1398
Psp5II	RGGWCCY	1 cut(s)	1472
Psp6I	CCWGG	9 cut(s)	1474, 2689, 3205, 3226, 3274, 3524, 3778, 3787, 4243
PspCI	CACGTG	1 cut(s)	1046
PspFI	CCCAGC	4 cut(s)	144, 680, 4181, 4214
PspGI	CCWGG	9 cut(s)	1474, 2689, 3205, 3226, 3274, 3524, 3778, 3787, 4243
PspOMI	GGGCCC	1 cut(s)	3521
PspPPI	RGGWCCY	1 cut(s)	1472
PspXI	VCTCGAGB	1 cut(s)	1672
PsrI	GAACNNNNNNTAC	2 cut(s)	2874, 2906
PstNI	CAGNNNCTG	3 cut(s)	19, 1127, 4829
PsuI	RGATCY	6 cut(s)	460, 2475, 3010, 3328, 4048, 4632
PsyI	GACNNNGTC	1 cut(s)	2614
PvuI	CGATCG	1 cut(s)	2845
PvuII	CAGCTG	5 cut(s)	37, 3175, 4189, 4768, 4826
RruI	TCGCGA	1 cut(s)	2820
RsaI	GTAC	6 cut(s)	749, 3662, 3985, 4163, 4524, 4748
RsaNI	GTAC	6 cut(s)	748, 3661, 3984, 4162, 4523, 4747
RseI	CAYNNNNRTG	7 cut(s)	519, 1069, 1080, 2963, 3474, 3516, 4491
ScaI	AGTACT	1 cut(s)	4748
ScrFI	CCNGG	9 cut(s)	1476, 2691, 3207, 3228, 3276, 3526, 3780, 3789, 4245
SduI	GDGCHC	7 cut(s)	192, 1953, 2506, 2851, 3525, 3772, 4721
SfcI	CTRYAG	4 cut(s)	648, 2952, 3321, 3690
Sfr274I	CTCGAG	1 cut(s)	1672
SinI	GGWCC	7 cut(s)	200, 271, 1301, 1472, 1590, 1910, 4019
SlaI	CTCGAG	1 cut(s)	1672
SmiMI	CAYNNNNRTG	7 cut(s)	519, 1069, 1080, 2963, 3474, 3516, 4491
SmlI	CTYRAG	3 cut(s)	1672, 1967, 1979
SmoI	CTYRAG	3 cut(s)	1672, 1967, 1979
SphI	GCATGC	1 cut(s)	3515
SspI	AATATT	3 cut(s)	589, 1897, 4255
SspMI	CTAG	6 cut(s)	957, 1374, 1469, 3533, 3797, 4779
StyD4I	CCNGG	9 cut(s)	1474, 2689, 3205, 3226, 3274, 3524, 3778, 3787, 4243
StyI	CCWWGG	4 cut(s)	196, 455, 2049, 4608
TaiI	ACGT	3 cut(s)	1048, 1401, 3136
TatI	WGTACW	3 cut(s)	747, 4522, 4746
TauI	GCSGC	3 cut(s)	8, 60, 1148
TfiI	GAWTC	7 cut(s)	315, 1132, 1195, 1442, 2898, 3997, 4595
TseFI	GTSAC	9 cut(s)	15, 896, 992, 1062, 2916, 3142, 4166, 4318, 4837
Tsp45I	GTSAC	9 cut(s)	15, 896, 992, 1062, 2916, 3142, 4166, 4318, 4837
TspGWI	ACGGA	4 cut(s)	150, 169, 1014, 3898
Tth111I	GACNNNGTC	1 cut(s)	2614
Vha464I	CTTAAG	1 cut(s)	1979
VneI	GTGCAC	1 cut(s)	2502
VpaK11BI	GGWCC	7 cut(s)	200, 271, 1301, 1472, 1590, 1910, 4019
XagI	CCTNNNNNAGG	2 cut(s)	3210, 3356
XapI	RAATTY	4 cut(s)	128, 1151, 2457, 2665
XceI	RCATGY	5 cut(s)	1347, 1588, 2337, 3515, 3841
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1566
XhoI	CTCGAG	1 cut(s)	1672
XmnI	GAANNNNTTC	1 cut(s)	2633
XspI	CTAG	6 cut(s)	957, 1374, 1469, 3533, 3797, 4779
ZrmI	AGTACT	1 cut(s)	4748
Zsp2I	ATGCAT	1 cut(s)	1924

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.