MD09G1246300.v1.1

Ubiquitin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004095
Physical Location & Seq
Forward (+)
31563726 .. 31564499
774 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1246300.v1.1.491

Sequence Viewer

Length: 774 bp
ATGAGGGTGATCATTACTTCATGGGGACACAAATTTACCATGGAAGTGAATGTCAAGGAACCAGTTGTTGAAATCAAAAGGAAGATAGAACAAGTGTTGGGTGTCCCTTTGGCTTCCCAAACCCTTGCAGTGTGTGGCTGGGAGTTAATAGATGGATTAGACATGGAAGATTACCCAATTGTCACTGAAGGTACTACCATTGACCTCACCATCAAATGGACTGGATTCGGAACTCCTTCGAATTTATACCATAATTCTAGTGGTAAAATTCAAATTACCATCAAGTTTTCAGCAAGGCAGCTTCCCATGGAGGTTGATGCATCCGAAACAGTCCGTAGCCTTAAAGAAAAAATACATATCATGGACGGTACCCCGATCAAGAGGATGTCGCTCTTCTTCTGTGGAATTGAGTTAGACGAAGATTTTCGCAATCTAAGTGAGTACGGGATACATTCATTTTCGGAGATTGTTGTGTTCCTCAAGGCTATGAGTCGTCTCAGAGATGAGCCGACGGCAAGAAGGTTGAGTGTAGTGCTACAAACATCGTCAAGTTTACTCAACGCGGCAACGATTCCATTGGAAGTGAAGGATTTGAGCACGGTTAATGAGTTGAGGCAATTGCTTTTGAGCAGGAAAATTCTGCCAATGGATGATTACTTGTTTATACACAAGCAGAGGATCATGCGTGACAATTGCAGCCTCAGGTGGCATGGTGTTGGTGATGGAGATTTCCTATATGTGTTTAAAGGGACCCTTTGTCACAGCGAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

258

Amino Acids

29.25

Weight (kDa)

6.18

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 8 - 72 8.4e-08 Ubiquitin family
ubiquitin PF00240 92 - 161 1.5e-13 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 368
AccB7I CCANNNNNTGG 1 cut(s) 216
AccII CGCG 1 cut(s) 563
AciI CCGC 1 cut(s) 563
AclWI GGATC 1 cut(s) 686
AcsI RAATTY 4 cut(s) 32, 241, 267, 636
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 3 cut(s) 193, 370, 443
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 2 cut(s) 71, 272
AhdI GACNNNNNGTC 1 cut(s) 756
AluBI AGCT 1 cut(s) 301
AluI AGCT 1 cut(s) 301
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 686
ApeKI GCWGC 2 cut(s) 298, 696
ApoI RAATTY 4 cut(s) 32, 241, 267, 636
Asp700I GAANNNNTTC 2 cut(s) 235, 423
Asp718I GGTACC 1 cut(s) 368
AspS9I GGNCC 1 cut(s) 750
AsuHPI GGTGA 3 cut(s) 19, 199, 731
AsuII TTCGAA 1 cut(s) 239
AvaII GGWCC 1 cut(s) 750
AxyI CCTNAGG 1 cut(s) 701
BanI GGYRCC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 599
BbvI GCAGC 2 cut(s) 310, 708
BccI CCATC 4 cut(s) 146, 218, 287, 716
BceAI ACGGC 1 cut(s) 528
BciVI GTATCC 1 cut(s) 441
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 258
BfuI GTATCC 1 cut(s) 441
BisI GCNGC 3 cut(s) 299, 564, 697
BlsI GCNGC 3 cut(s) 300, 565, 698
Bme18I GGWCC 1 cut(s) 750
BmeRI GACNNNNNGTC 1 cut(s) 756
BmgT120I GGNCC 1 cut(s) 750
BmiI GGNNCC 4 cut(s) 60, 370, 751, 752
BmsI GCATC 2 cut(s) 307, 329
Bpu14I TTCGAA 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 464
BsaJI CCNNGG 2 cut(s) 39, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse1I ACTGG 2 cut(s) 62, 226
Bse21I CCTNAGG 1 cut(s) 701
BseDI CCNNGG 2 cut(s) 39, 306
BseGI GGATG 3 cut(s) 320, 390, 655
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 511, 715
BseNI ACTGG 2 cut(s) 62, 226
BseXI GCAGC 2 cut(s) 310, 708
BseYI CCCAGC 1 cut(s) 138
Bsh1236I CGCG 1 cut(s) 563
BshNI GGYRCC 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 599
BslFI GGGAC 3 cut(s) 39, 89, 763
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 1 cut(s) 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 3 cut(s) 39, 89, 763
BsmI GAATGC 1 cut(s) 773
Bsp119I TTCGAA 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 599
Bsp143I GATC 3 cut(s) 9, 375, 678
Bsp19I CCATGG 2 cut(s) 39, 306
BspACI CCGC 1 cut(s) 563
BspCNI CTCAG 2 cut(s) 510, 714
BspFNI CGCG 1 cut(s) 563
BspLI GGNNCC 4 cut(s) 60, 370, 751, 752
BspPI GGATC 1 cut(s) 686
BspQI GCTCTTC 1 cut(s) 398
BspT104I TTCGAA 1 cut(s) 239
BspT107I GGYRCC 1 cut(s) 368
BsrI ACTGG 2 cut(s) 62, 226
BssECI CCNNGG 2 cut(s) 39, 306
BssMI GATC 3 cut(s) 9, 375, 678
BssT1I CCWWGG 2 cut(s) 39, 306
Bst4CI ACNGT 3 cut(s) 331, 368, 601
Bst6I CTCTTC 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 239
BstDEI CTNAG 3 cut(s) 434, 497, 701
BstDSI CCRYGG 2 cut(s) 39, 306
BstF5I GGATG 3 cut(s) 320, 390, 655
BstFNI CGCG 1 cut(s) 563
BstKTI GATC 3 cut(s) 12, 378, 681
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 3 cut(s) 9, 375, 678
BstUI CGCG 1 cut(s) 563
BstV1I GCAGC 2 cut(s) 310, 708
Bsu36I CCTNAGG 1 cut(s) 701
BsuI GTATCC 1 cut(s) 441
BtgI CCRYGG 2 cut(s) 39, 306
BtsCI GGATG 3 cut(s) 320, 390, 655
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 135, 183
Cfr13I GGNCC 1 cut(s) 750
Csp6I GTAC 3 cut(s) 192, 369, 442
CviAII CATG 7 cut(s) 21, 40, 163, 307, 361, 682, 710
CviJI RGCY 7 cut(s) 113, 138, 301, 339, 485, 508, 699
CviKI_1 RGCY 7 cut(s) 113, 138, 301, 339, 485, 508, 699
CviQI GTAC 3 cut(s) 192, 369, 442
DdeI CTNAG 3 cut(s) 434, 497, 701
DpnI GATC 3 cut(s) 11, 377, 680
DpnII GATC 3 cut(s) 9, 375, 678
DraI TTTAAA 1 cut(s) 745
DriI GACNNNNNGTC 1 cut(s) 756
Eam1104I CTCTTC 1 cut(s) 398
Eam1105I GACNNNNNGTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 398
Eco130I CCWWGG 2 cut(s) 39, 306
Eco47I GGWCC 1 cut(s) 750
Eco57I CTGAAG 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 701
EcoO109I RGGNCCY 1 cut(s) 750
EcoT14I CCWWGG 2 cut(s) 39, 306
EcoT22I ATGCAT 1 cut(s) 322
ErhI CCWWGG 2 cut(s) 39, 306
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 7 cut(s) 24, 43, 166, 310, 364, 685, 713
FalI AAGNNNNNCTT 2 cut(s) 738, 770
FaqI GGGAC 3 cut(s) 39, 89, 763
FatI CATG 7 cut(s) 20, 39, 162, 306, 360, 681, 709
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 3 cut(s) 299, 564, 697
FokI GGATG 3 cut(s) 307, 397, 662
Fsp4HI GCNGC 3 cut(s) 299, 564, 697
FspBI CTAG 1 cut(s) 258
GluI GCNGC 3 cut(s) 299, 564, 697
GsaI CCCAGC 1 cut(s) 142
Hin1II CATG 7 cut(s) 24, 43, 166, 310, 364, 685, 713
HinfI GANTC 3 cut(s) 225, 490, 571
HphI GGTGA 3 cut(s) 19, 199, 731
Hpy166II GTNNAC 1 cut(s) 554
Hpy188I TCNGA 4 cut(s) 230, 325, 463, 500
Hpy188III TCNNGA 1 cut(s) 379
Hpy8I GTNNAC 1 cut(s) 554
Hpy99I CGWCG 1 cut(s) 514
HpyAV CCTTC 4 cut(s) 182, 246, 513, 580
HpyCH4III ACNGT 3 cut(s) 331, 368, 601
HpyCH4V TGCA 3 cut(s) 128, 320, 696
HpyF3I CTNAG 3 cut(s) 434, 497, 701
Hsp92II CATG 7 cut(s) 24, 43, 166, 310, 364, 685, 713
KflI GGGWCCC 1 cut(s) 750
KpnI GGTACC 1 cut(s) 372
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 3 cut(s) 9, 375, 678
LguI GCTCTTC 1 cut(s) 398
LpnPI CCDG 5 cut(s) 75, 124, 207, 616, 688
Lsp1109I GCAGC 2 cut(s) 310, 708
LweI GCATC 2 cut(s) 307, 329
MaeI CTAG 1 cut(s) 258
MaeIII GTNAC 3 cut(s) 181, 686, 758
MalI GATC 3 cut(s) 11, 377, 680
MboI GATC 3 cut(s) 9, 375, 678
MboII GAAGA 5 cut(s) 94, 179, 385, 388, 431
MfeI CAATTG 3 cut(s) 177, 617, 691
MhlI GDGCHC 1 cut(s) 599
MlyI GAGTC 1 cut(s) 499
MnlI CCTC 7 cut(s) 215, 304, 375, 488, 606, 669, 710
Mph1103I ATGCAT 1 cut(s) 322
MroXI GAANNNNTTC 2 cut(s) 235, 423
MseI TTAA 4 cut(s) 146, 342, 603, 744
MslI CAYNNNNRTG 1 cut(s) 44
MunI CAATTG 3 cut(s) 177, 617, 691
Mva1269I GAATGC 1 cut(s) 773
MvnI CGCG 1 cut(s) 563
NcoI CCATGG 2 cut(s) 39, 306
NdeII GATC 3 cut(s) 9, 375, 678
NlaIII CATG 7 cut(s) 24, 43, 166, 310, 364, 685, 713
NlaIV GGNNCC 4 cut(s) 60, 370, 751, 752
NmuCI GTSAC 3 cut(s) 181, 686, 758
NsiI ATGCAT 1 cut(s) 322
NspV TTCGAA 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 398
PctI GAATGC 1 cut(s) 773
PdmI GAANNNNTTC 2 cut(s) 235, 423
PfeI GAWTC 2 cut(s) 225, 571
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 3 cut(s) 300, 565, 698
PleI GAGTC 1 cut(s) 498
PpsI GAGTC 1 cut(s) 498
PpuMI RGGWCCY 1 cut(s) 750
Psp5II RGGWCCY 1 cut(s) 750
PspFI CCCAGC 1 cut(s) 138
PspN4I GGNNCC 4 cut(s) 60, 370, 751, 752
PspPI GGNCC 1 cut(s) 750
PspPPI RGGWCCY 1 cut(s) 750
RsaI GTAC 3 cut(s) 193, 370, 443
RsaNI GTAC 3 cut(s) 192, 369, 442
RseI CAYNNNNRTG 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 398
SaqAI TTAA 4 cut(s) 146, 342, 603, 744
SatI GCNGC 3 cut(s) 299, 564, 697
Sau3AI GATC 3 cut(s) 9, 375, 678
Sau96I GGNCC 1 cut(s) 750
SchI GAGTC 1 cut(s) 499
SduI GDGCHC 1 cut(s) 599
SetI ASST 6 cut(s) 193, 207, 303, 315, 524, 707
SfaNI GCATC 2 cut(s) 307, 329
SfuI TTCGAA 1 cut(s) 239
SinI GGWCC 1 cut(s) 750
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SsiI CCGC 1 cut(s) 563
SspMI CTAG 1 cut(s) 258
StyI CCWWGG 2 cut(s) 39, 306
TaaI ACNGT 3 cut(s) 331, 368, 601
TaqI TCGA 1 cut(s) 239
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 2 cut(s) 225, 571
Tru1I TTAA 4 cut(s) 146, 342, 603, 744
Tru9I TTAA 4 cut(s) 146, 342, 603, 744
TscAI CASTG 2 cut(s) 135, 190
TseFI GTSAC 3 cut(s) 181, 686, 758
TseI GCWGC 2 cut(s) 298, 696
Tsp45I GTSAC 3 cut(s) 181, 686, 758
TspDTI ATGAA 2 cut(s) 9, 444
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 2 cut(s) 135, 190
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 750
XapI RAATTY 4 cut(s) 32, 241, 267, 636
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmnI GAANNNNTTC 2 cut(s) 235, 423
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.