Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003001

Physical Location & Seq

Genomic Coordinates Forward (+)

35408751 .. 35415509

Gene Structure 6759 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1277400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1293 bp

ATGGGTTTAGCATTCACAGGCAGCAGCGGAGCAAGCATGAGGTACTTGCCCACCCTCTCAAACCCACACTCGCACTCGCACTGCTTACCTTCTCCTCTACTCTTCCACTCTTCTCTCCCGCAGCGCTTCAACCTACACTCCATGGCGGTGAAGCCAATCAAAAGGGTTCGTTGCGAGCTCCAAGCCAAAGTCAATGGCGCTCTTTCCGCCGACTCCGACCCCCGTTTCATCGACCGGCAAAAAGCACTGGAAGCGGCAATGAATGATATAAATAGTTCATTTGGGAAGGGAAGTGTTACAAGATTAGGAAGTGCCGGTGGAGCTCTGGTTGAAACTTTTCCCAGTGGCTGCTTGACATTAGACCTTGCCTTAGGTGGTGGCCTTCCAAAAGGGAGAATTGTGGAGATATTTGGACCTGAAAGTAGTGGAAAAACCACTCTAGCACTCCATGCAATTGCAGAGGTGCAGAAACTTGGAGGAAATGCTATGCTTGTTGATGCAGAACATGCTTTTGATCCAGCATATTCAAGAGCACTGGGAGTAGATGTAGAAAATTTGATTGTTTGTCAGCCTGATCATGGTGAAATGGCACTTGAGATTGCAGATCGTATGTGCAGATCTGGTGCCATTGATCTTATCTGTGTTGATTCTGTCTCGGCTCTCACTCCACGAGCAGAGATTGAAGGTGAGATAGGGATGCAGCAAATGGGTTTGCAAGCACGTCTTATGAGCCAAGCTTTGCGCAAAATGTCAGGAAATGCATCTAAAGCTGGATGTACACTAATATTTTTAAATCAAATCAGATATAAGATCGGTGTATATTATGGGAATCCAGAAGTGACATCTGGAGGAATTGCATTAAAATTCTTTGCCTCAGTTCGGCTTGAAATACGTTCTACAGGGAAGATAAAGTCTGTAAAGGGAGATGAAGAAATCGGGGTTCGGGTTCGTGTAAGAGTAGTAAAGAGTAAGGTTTCGAGACCATACAAACAAGCTGAATTTGAAATTGTTTTTGGAGAGGGAGTTAGTAAATTGGGTTGCATCTTGGATTGTGCTGAAGCAATGGATGTAGTAGTGAGGAAGGGTTCTTGGTATAGCTATGGGGACCACAGGTTAGGACAAGGAAGAGATAAAGCATTGCAGTACTTGAGAGAGAACCCTCTTCTTCTTGATGAAATGGAGAAGATAGTTCGCTCCATGATGACAGATGGAACTGGACAAGTAGGCTCATTTCAGACAAGTAACTTGTCACTTGCCCAACAGGATGAAGATGATTTTGAGGATATCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000150 GO:0000217 GO:0000400 GO:0000724 GO:0000725 GO:0000730 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009987 GO:0010212 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0033554 GO:0034622 GO:0034641 GO:0042148 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045003 GO:0046483 GO:0050896 GO:0051716 GO:0065003 GO:0065004 GO:0071704 GO:0071824 GO:0071840 GO:0090304 GO:0090735 GO:0097159 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03440 map03440

Pfam Domains

Protein Families

RecA

Protein Analysis

431

Amino Acids

46.5

Weight (kDa)

6.77

Isoelectric Point (pI)

30.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RecA_N	PF00154	81 - 343	2.9e-116	RecA N-terminal domain
Rad51	PF08423	115 - 299	3.8e-07	Rad51
RecA_C	PF21096	346 - 400	4.9e-21	RecA C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79050 AT1G79050
fragaria_vesca	FvH4_1g27813
malus_domestica	MD09G1277400.v1.1 MD17G1270800.v1.1
prunus_persica	Prupe.3G015400_v2.0.a1 Prupe.3G015400_v2.0.a1 Prupe.3G015400_v2.0.a1 Prupe.3G015400_v2.0.a1
pyrus_communis	pycom09g18680 pycom17g26930
rosa_chinensis	RchiOBHm_Chr1g0376401 RchiOBHm_Chr3g0485071 RchiOBHm_Chr3g0485081 RchiOBHm_Chr3g0488901 RchiOBHm_Chr3g0488941 RchiOBHm_Chr3g0488951 RchiOBHm_Chr6g0270261
rosa_laevigata	RLG00000001730 RLG00000022906
rosa_multiflora	Rmu_sc0001208.1_g000006
rosa_roxburghii	Rroxscaffold_5G00371550 Rroxscaffold_6G00393780
rosa_rugosa	Rorug03G0242300
rosa_samantha	Rh2CG293500 Rh3AG266100 Rh3AG290500 Rh3BG327500 Rh3CG324800 Rh3DG323400 Rh3DG323700 Rh3DG329000 Rh5AG166700
rosa_wichuraiana	Rw1G015550 Rw1G026850 Rw3G025730 Rw5G015170 Rw7G021290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	743
AccB1I	GGYRCC	1 cut(s)	623
AciI	CCGC	5 cut(s)	27, 119, 146, 207, 254
AclWI	GGATC	1 cut(s)	509
AcsI	RAATTY	3 cut(s)	553, 863, 998
AcuI	CTGAAG	1 cut(s)	1077
AfaI	GTAC	3 cut(s)	44, 778, 1145
AfeI	AGCGCT	1 cut(s)	125
AfiI	CCNNNNNNNGG	2 cut(s)	578, 879
AgsI	TTSAA	6 cut(s)	130, 332, 528, 683, 887, 1004
AjiI	CACGTC	1 cut(s)	722
AjuI	GAANNNNNNNTTGG	2 cut(s)	996, 1028
AluBI	AGCT	6 cut(s)	178, 323, 737, 770, 995, 1098
AluI	AGCT	6 cut(s)	178, 323, 737, 770, 995, 1098
Alw21I	GWGCWC	3 cut(s)	180, 325, 535
Alw26I	GTCTC	2 cut(s)	658, 973
AlwI	GGATC	1 cut(s)	509
AlwNI	CAGNNNCTG	1 cut(s)	348
Aor51HI	AGCGCT	1 cut(s)	125
AoxI	GGCC	1 cut(s)	379
ApeKI	GCWGC	5 cut(s)	21, 24, 121, 348, 700
ApoI	RAATTY	3 cut(s)	553, 863, 998
Asp700I	GAANNNNTTC	1 cut(s)	336
AspLEI	GCGC	3 cut(s)	126, 200, 744
AspS9I	GGNCC	2 cut(s)	413, 1105
AsuHPI	GGTGA	3 cut(s)	160, 593, 698
AvaII	GGWCC	2 cut(s)	413, 1105
AxyI	CCTNAGG	1 cut(s)	370
BanI	GGYRCC	1 cut(s)	623
BanII	GRGCYC	2 cut(s)	180, 325
BauI	CACGAG	1 cut(s)	669
Bbv12I	GWGCWC	3 cut(s)	180, 325, 535
BbvI	GCAGC	5 cut(s)	33, 36, 133, 335, 712
BccI	CCATC	1 cut(s)	1202
BclI	TGATCA	1 cut(s)	574
BcoDI	GTCTC	2 cut(s)	658, 973
BfaI	CTAG	1 cut(s)	440
BfmI	CTRYAG	1 cut(s)	897
BfoI	RGCGCY	2 cut(s)	127, 201
BglII	AGATCT	1 cut(s)	617
BisI	GCNGC	6 cut(s)	22, 25, 122, 255, 349, 701
BlsI	GCNGC	6 cut(s)	23, 26, 123, 256, 350, 702
BmcAI	AGTACT	1 cut(s)	1145
Bme18I	GGWCC	2 cut(s)	413, 1105
BmgBI	CACGTC	1 cut(s)	722
BmgT120I	GGNCC	2 cut(s)	413, 1105
BmiI	GGNNCC	2 cut(s)	625, 1106
BmrI	ACTGGG	2 cut(s)	336, 545
BmsI	GCATC	4 cut(s)	487, 687, 770, 1050
BmuI	ACTGGG	2 cut(s)	336, 545
BpmI	CTGGAG	1 cut(s)	867
BpuEI	CTTGAG	2 cut(s)	614, 1168
BsaI	GGTCTC	1 cut(s)	973
BsaJI	CCNNGG	1 cut(s)	141
BsaXI	ACNNNNNCTCC	8 cut(s)	122, 152, 312, 342, 531, 561, 1008, 1038
Bsc4I	CCNNNNNNNGG	2 cut(s)	578, 879
Bse118I	RCCGGY	2 cut(s)	234, 314
Bse1I	ACTGG	4 cut(s)	252, 342, 540, 1219
Bse21I	CCTNAGG	1 cut(s)	370
Bse3DI	GCAATG	3 cut(s)	264, 1068, 1136
BseDI	CCNNGG	1 cut(s)	141
BseGI	GGATG	4 cut(s)	702, 779, 1072, 1270
BseLI	CCNNNNNNNGG	2 cut(s)	578, 879
BseMI	GCAATG	3 cut(s)	264, 1068, 1136
BseMII	CTCAG	1 cut(s)	888
BseNI	ACTGG	4 cut(s)	252, 342, 540, 1219
BseRI	GAGGAG	1 cut(s)	84
BseXI	GCAGC	5 cut(s)	33, 36, 133, 335, 712
BsgI	GTGCAG	2 cut(s)	485, 634
Bsh1285I	CGRYCG	1 cut(s)	235
BshFI	GGCC	1 cut(s)	381
BshNI	GGYRCC	1 cut(s)	623
BsiEI	CGRYCG	1 cut(s)	235
BsiHKAI	GWGCWC	3 cut(s)	180, 325, 535
BsiSI	CCGG	2 cut(s)	235, 315
BslFI	GGGAC	1 cut(s)	1118
BslI	CCNNNNNNNGG	2 cut(s)	578, 879
BsmAI	GTCTC	2 cut(s)	658, 973
BsmFI	GGGAC	1 cut(s)	1118
BsmI	GAATGC	1 cut(s)	11
BsnI	GGCC	1 cut(s)	381
Bso31I	GGTCTC	1 cut(s)	973
Bsp1286I	GDGCHC	3 cut(s)	180, 325, 535
Bsp1407I	TGTACA	1 cut(s)	776
Bsp143I	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
Bsp19I	CCATGG	1 cut(s)	141
BspACI	CCGC	5 cut(s)	27, 119, 146, 207, 254
BspANI	GGCC	1 cut(s)	381
BspCNI	CTCAG	1 cut(s)	887
BspLI	GGNNCC	2 cut(s)	625, 1106
BspPI	GGATC	1 cut(s)	509
BspT107I	GGYRCC	1 cut(s)	623
BspTNI	GGTCTC	1 cut(s)	973
BsrDI	GCAATG	3 cut(s)	264, 1068, 1136
BsrFI	RCCGGY	2 cut(s)	234, 314
BsrGI	TGTACA	1 cut(s)	776
BsrI	ACTGG	4 cut(s)	252, 342, 540, 1219
BssAI	RCCGGY	2 cut(s)	234, 314
BssECI	CCNNGG	1 cut(s)	141
BssMI	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
BssSI	CACGAG	1 cut(s)	669
BssT1I	CCWWGG	1 cut(s)	141
Bst2BI	CACGAG	1 cut(s)	669
Bst6I	CTCTTC	4 cut(s)	107, 115, 1120, 1167
BstAPI	GCANNNNNTGC	2 cut(s)	449, 506
BstAUI	TGTACA	1 cut(s)	776
BstC8I	GCNNGC	3 cut(s)	34, 176, 717
BstDEI	CTNAG	2 cut(s)	370, 874
BstDSI	CCRYGG	1 cut(s)	141
BstF5I	GGATG	4 cut(s)	702, 779, 1072, 1270
BstH2I	RGCGCY	2 cut(s)	127, 201
BstHHI	GCGC	3 cut(s)	126, 200, 744
BstKTI	GATC	6 cut(s)	517, 577, 607, 620, 634, 813
BstMAI	GTCTC	2 cut(s)	658, 973
BstMBI	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
BstMCI	CGRYCG	1 cut(s)	235
BstMWI	GCNNNNNNNGC	7 cut(s)	33, 206, 251, 320, 449, 506, 767
BstNSI	RCATGY	1 cut(s)	509
BstSFI	CTRYAG	1 cut(s)	897
BstV1I	GCAGC	5 cut(s)	33, 36, 133, 335, 712
BstX2I	RGATCY	1 cut(s)	617
BstYI	RGATCY	1 cut(s)	617
Bsu36I	CCTNAGG	1 cut(s)	370
BsuRI	GGCC	1 cut(s)	381
BtgI	CCRYGG	1 cut(s)	141
BtrI	CACGTC	1 cut(s)	722
BtsCI	GGATG	4 cut(s)	702, 779, 1072, 1270
BtsI	GCAGTG	1 cut(s)	79
BtsIMutI	CAGTG	4 cut(s)	79, 245, 349, 533
Cac8I	GCNNGC	3 cut(s)	34, 176, 717
CaiI	CAGNNNCTG	1 cut(s)	348
CfoI	GCGC	3 cut(s)	126, 200, 744
Cfr10I	RCCGGY	2 cut(s)	234, 314
Cfr13I	GGNCC	2 cut(s)	413, 1105
Csp6I	GTAC	3 cut(s)	43, 777, 1144
CviAII	CATG	6 cut(s)	37, 142, 449, 506, 578, 1198
CviQI	GTAC	3 cut(s)	43, 777, 1144
DdeI	CTNAG	2 cut(s)	370, 874
DpnI	GATC	6 cut(s)	516, 576, 606, 619, 633, 812
DpnII	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
DraI	TTTAAA	1 cut(s)	792
Eam1104I	CTCTTC	4 cut(s)	107, 115, 1120, 1167
EarI	CTCTTC	4 cut(s)	107, 115, 1120, 1167
EciI	GGCGGA	1 cut(s)	196
Ecl136II	GAGCTC	2 cut(s)	178, 323
Eco130I	CCWWGG	1 cut(s)	141
Eco24I	GRGCYC	2 cut(s)	180, 325
Eco31I	GGTCTC	1 cut(s)	973
Eco32I	GATATC	1 cut(s)	1285
Eco47I	GGWCC	2 cut(s)	413, 1105
Eco47III	AGCGCT	1 cut(s)	125
Eco53kI	GAGCTC	2 cut(s)	178, 323
Eco57I	CTGAAG	1 cut(s)	1077
Eco81I	CCTNAGG	1 cut(s)	370
EcoICRI	GAGCTC	2 cut(s)	178, 323
EcoRV	GATATC	1 cut(s)	1285
EcoT14I	CCWWGG	1 cut(s)	141
EcoT22I	ATGCAT	1 cut(s)	763
EcoT38I	GRGCYC	2 cut(s)	180, 325
ErhI	CCWWGG	1 cut(s)	141
FaeI	CATG	6 cut(s)	40, 145, 452, 509, 581, 1201
FalI	AAGNNNNNCTT	2 cut(s)	708, 740
FaqI	GGGAC	1 cut(s)	1118
FatI	CATG	6 cut(s)	36, 141, 448, 505, 577, 1197
FauI	CCCGC	1 cut(s)	126
FbaI	TGATCA	1 cut(s)	574
Fnu4HI	GCNGC	6 cut(s)	22, 25, 122, 255, 349, 701
FokI	GGATG	4 cut(s)	709, 786, 1079, 1277
FriOI	GRGCYC	2 cut(s)	180, 325
Fsp4HI	GCNGC	6 cut(s)	22, 25, 122, 255, 349, 701
FspBI	CTAG	1 cut(s)	440
FspI	TGCGCA	1 cut(s)	743
GlaI	GCGC	3 cut(s)	125, 199, 743
GluI	GCNGC	6 cut(s)	22, 25, 122, 255, 349, 701
GsuI	CTGGAG	1 cut(s)	867
HaeII	RGCGCY	2 cut(s)	127, 201
HaeIII	GGCC	1 cut(s)	381
HapII	CCGG	2 cut(s)	235, 315
HhaI	GCGC	3 cut(s)	126, 200, 744
Hin1II	CATG	6 cut(s)	40, 145, 452, 509, 581, 1201
Hin6I	GCGC	3 cut(s)	124, 198, 742
HinP1I	GCGC	3 cut(s)	124, 198, 742
HindIII	AAGCTT	1 cut(s)	735
HinfI	GANTC	3 cut(s)	212, 647, 829
HpaII	CCGG	2 cut(s)	235, 315
HphI	GGTGA	3 cut(s)	160, 593, 698
Hpy166II	GTNNAC	1 cut(s)	779
Hpy188I	TCNGA	3 cut(s)	217, 803, 1236
Hpy188III	TCNNGA	6 cut(s)	528, 753, 833, 846, 978, 1169
Hpy8I	GTNNAC	1 cut(s)	779
HpyAV	CCTTC	5 cut(s)	99, 280, 392, 677, 1075
HpyCH4IV	ACGT	2 cut(s)	721, 892
HpyF10VI	GCNNNNNNNGC	7 cut(s)	33, 206, 251, 320, 449, 506, 767
HpyF3I	CTNAG	2 cut(s)	370, 874
HpySE526I	ACGT	2 cut(s)	721, 892
Hsp92II	CATG	6 cut(s)	40, 145, 452, 509, 581, 1201
HspAI	GCGC	3 cut(s)	124, 198, 742
Ksp22I	TGATCA	1 cut(s)	574
Kzo9I	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
LmnI	GCTCC	4 cut(s)	29, 183, 320, 1199
Lsp1109I	GCAGC	5 cut(s)	33, 36, 133, 335, 712
LweI	GCATC	4 cut(s)	487, 687, 770, 1050
MaeI	CTAG	1 cut(s)	440
MaeII	ACGT	2 cut(s)	721, 892
MaeIII	GTNAC	4 cut(s)	295, 838, 1241, 1248
MalI	GATC	6 cut(s)	516, 576, 606, 619, 633, 812
MboI	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
MboII	GAAGA	9 cut(s)	94, 102, 916, 941, 1137, 1154, 1157, 1195, 1280
MfeI	CAATTG	1 cut(s)	453
MflI	RGATCY	1 cut(s)	617
MhlI	GDGCHC	3 cut(s)	180, 325, 535
MluCI	AATT	8 cut(s)	396, 453, 553, 852, 863, 998, 1005, 1031
MlyI	GAGTC	1 cut(s)	206
MmeI	TCCRAC	1 cut(s)	240
Mph1103I	ATGCAT	1 cut(s)	763
MroXI	GAANNNNTTC	1 cut(s)	336
MseI	TTAA	2 cut(s)	791, 860
MslI	CAYNNNNRTG	1 cut(s)	146
MspA1I	CMGCKG	1 cut(s)	27
MspI	CCGG	2 cut(s)	235, 315
MunI	CAATTG	1 cut(s)	453
Mva1269I	GAATGC	1 cut(s)	11
MwoI	GCNNNNNNNGC	7 cut(s)	33, 206, 251, 320, 449, 506, 767
NcoI	CCATGG	1 cut(s)	141
NdeII	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
NlaIII	CATG	6 cut(s)	40, 145, 452, 509, 581, 1201
NlaIV	GGNNCC	2 cut(s)	625, 1106
NmeAIII	GCCGAG	1 cut(s)	635
NmuCI	GTSAC	2 cut(s)	838, 1248
NsbI	TGCGCA	1 cut(s)	743
NsiI	ATGCAT	1 cut(s)	763
NspI	RCATGY	1 cut(s)	509
PctI	GAATGC	1 cut(s)	11
PdmI	GAANNNNTTC	1 cut(s)	336
PfeI	GAWTC	2 cut(s)	647, 829
PkrI	GCNGC	6 cut(s)	23, 26, 123, 256, 350, 702
PleI	GAGTC	1 cut(s)	206
PpsI	GAGTC	1 cut(s)	206
Psp124BI	GAGCTC	2 cut(s)	180, 325
PspN4I	GGNNCC	2 cut(s)	625, 1106
PspPI	GGNCC	2 cut(s)	413, 1105
PstNI	CAGNNNCTG	1 cut(s)	348
PsuI	RGATCY	1 cut(s)	617
RsaI	GTAC	3 cut(s)	44, 778, 1145
RsaNI	GTAC	3 cut(s)	43, 777, 1144
RseI	CAYNNNNRTG	1 cut(s)	146
SacI	GAGCTC	2 cut(s)	180, 325
SaqAI	TTAA	2 cut(s)	791, 860
SatI	GCNGC	6 cut(s)	22, 25, 122, 255, 349, 701
Sau3AI	GATC	6 cut(s)	514, 574, 604, 617, 631, 810
Sau96I	GGNCC	2 cut(s)	413, 1105
ScaI	AGTACT	1 cut(s)	1145
SchI	GAGTC	1 cut(s)	206
SduI	GDGCHC	3 cut(s)	180, 325, 535
SfaNI	GCATC	4 cut(s)	487, 687, 770, 1050
SfcI	CTRYAG	1 cut(s)	897
SinI	GGWCC	2 cut(s)	413, 1105
SmiMI	CAYNNNNRTG	1 cut(s)	146
SmlI	CTYRAG	2 cut(s)	593, 1147
SmoI	CTYRAG	2 cut(s)	593, 1147
Sse9I	AATT	8 cut(s)	396, 453, 553, 852, 863, 998, 1005, 1031
SsiI	CCGC	5 cut(s)	27, 119, 146, 207, 254
SspI	AATATT	1 cut(s)	786
SspMI	CTAG	1 cut(s)	440
SstI	GAGCTC	2 cut(s)	180, 325
StyI	CCWWGG	1 cut(s)	141
TaiI	ACGT	2 cut(s)	724, 895
TaqI	TCGA	2 cut(s)	231, 977
TasI	AATT	8 cut(s)	396, 453, 553, 852, 863, 998, 1005, 1031
TatI	WGTACW	2 cut(s)	776, 1143
TauI	GCSGC	1 cut(s)	257
TfiI	GAWTC	2 cut(s)	647, 829
Tru1I	TTAA	2 cut(s)	791, 860
Tru9I	TTAA	2 cut(s)	791, 860
TscAI	CASTG	4 cut(s)	86, 252, 349, 540
TseFI	GTSAC	2 cut(s)	838, 1248
TseI	GCWGC	5 cut(s)	21, 24, 121, 348, 700
Tsp45I	GTSAC	2 cut(s)	838, 1248
TspDTI	ATGAA	6 cut(s)	217, 267, 275, 942, 1188, 1281
TspRI	CASTG	4 cut(s)	86, 252, 349, 540
VpaK11BI	GGWCC	2 cut(s)	413, 1105
XapI	RAATTY	3 cut(s)	553, 863, 998
XceI	RCATGY	1 cut(s)	509
XmnI	GAANNNNTTC	1 cut(s)	336
XspI	CTAG	1 cut(s)	440
ZrmI	AGTACT	1 cut(s)	1145
Zsp2I	ATGCAT	1 cut(s)	763

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.