Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0003001

Physical Location & Seq

Genomic Coordinates Reverse (-)

35291141 .. 35298102

Gene Structure 6962 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG323400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGGTTTAGCGCTGAGAAGCAACAGCGCAAGCATGACATATTATATGTCCACCACCCATTCACTCCCCTACTCACACTCTCCTCTCTCATTCCACTCCTGCTCTCTCCCACACCGACACCGCTTCAACGTTCAATCAATGGCGCTCAAAAGGGTCCGCTCTGAGCTCCAAGTGAAAGTCAACGGCTCTCTCTCCGCTGCTGACTCCGACCAACTCTTCCTCGACCGCCAAAAAGCACTGGAGGCGGCCATGAATGATATCAATAGCTCATTCGGCAAGGGAAGTGTTACAAGACTAGGAAGCGCTGGCGGAGCTTTGGTTGAAACTTTCCCCAGTGGTTGCTTGACATTGGACCTTGCCTTAGGTGGCGGCCTCCCAAAAGGGAGAATTGTAGAGATATTTGGACCAGAAAGTAGTGGAAAGACAACTCTAGCACTCCATGCAATTGCAGAGGTGCAGAGGCTTGGAGGAAATGCTATGCTTGTTGATGCAGAACATGCCTTTGATCCAGCATATTCAAAAGCATTGGGAGTAGATGTAGAAAATCTGATTGTCTGCCAGCCTGATCATGGTGAAATGGCACTTGAGATTGCAGATCGTATGTGCAGATCTGGTGCCATTGATCTTATCTGTGTCGATTCTGTCTCGGCTCTCACTCCACGAGCAGAAATTGAAGGTGAGATCGGGATGCAACAAATGGGTTTGCAAGCACGCCTTATGAGCCAAGCTTTGCGCAAAATGTCAGGAAATGCATCTAAAGCTGGATGTACTCTCATATTCTTGAATCAAATAAGATATAAGATTGGTGTGTATTATGGGAATCCAGAAGTGACATCTGGAGGAATTGCATTAAAGTTCTTTGCCTCAGTTCGCCTGGAAATACGTTCTACAGGGAAAATAAAATCTGTAAAAGGAGACGAGGAAATTGGGGTTCGGGTCCGTGTACGAGTGCAAAAGAGTAAGGTTTCGAGGCCATACAAACAAGCTGAATTTGAAATTGTGTTTGGAGAGGGAGTAAGTCAATTGGGTTGCATTTTGGATTGTGCTGAAATGATGGATGTAGTAGTGAAGAAGGGCTCTTGGTATAGCTATGGGGACCACAGGTTGGGACAAGGAAGAGATAAATCATTGCAGTACTTGAGAGAGAACCCTGTTCTTCTTGATGAGATAGAGAAGATAGTTCGCTCCACAATGACAGATGCAACTGGACAGGTAGGCTCATTGCACACAAGGAACTTGCCAGTAACACATCAGGATGAAGATGATTTTGAAGATGTCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000150 GO:0000217 GO:0000400 GO:0000724 GO:0000725 GO:0000730 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009987 GO:0010212 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0033554 GO:0034622 GO:0034641 GO:0042148 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045003 GO:0046483 GO:0050896 GO:0051716 GO:0065003 GO:0065004 GO:0071704 GO:0071824 GO:0071840 GO:0090304 GO:0090735 GO:0097159 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03440 map03440

Pfam Domains

Protein Families

RecA

Protein Analysis

427

Amino Acids

46.47

Weight (kDa)

6.61

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RecA_N	PF00154	78 - 340	7.5e-115	RecA N-terminal domain
Rad51	PF08423	112 - 296	4e-07	Rad51
RecA_C	PF21096	343 - 398	6.4e-22	RecA C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79050 AT1G79050
fragaria_vesca	FvH4_1g27813
malus_domestica	MD09G1277400.v1.1 MD17G1270800.v1.1
prunus_persica	Prupe.3G015400_v2.0.a1 Prupe.3G015400_v2.0.a1 Prupe.3G015400_v2.0.a1 Prupe.3G015400_v2.0.a1
pyrus_communis	pycom09g18680 pycom17g26930
rosa_chinensis	RchiOBHm_Chr1g0376401 RchiOBHm_Chr3g0485071 RchiOBHm_Chr3g0485081 RchiOBHm_Chr3g0488901 RchiOBHm_Chr3g0488941 RchiOBHm_Chr3g0488951 RchiOBHm_Chr6g0270261
rosa_laevigata	RLG00000001730 RLG00000022906
rosa_multiflora	Rmu_sc0001208.1_g000006
rosa_roxburghii	Rroxscaffold_5G00371550 Rroxscaffold_6G00393780
rosa_rugosa	Rorug03G0242300
rosa_samantha	Rh2CG293500 Rh3AG266100 Rh3AG290500 Rh3BG327500 Rh3CG324800 Rh3DG323400 Rh3DG323700 Rh3DG329000 Rh5AG166700
rosa_wichuraiana	Rw1G015550 Rw1G026850 Rw3G025730 Rw5G015170 Rw7G021290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	734
AccB1I	GGYRCC	1 cut(s)	614
AccB7I	CCANNNNNTGG	1 cut(s)	1105
AccBSI	CCGCTC	1 cut(s)	159
AciI	CCGC	7 cut(s)	121, 157, 195, 226, 245, 309, 369
AclI	AACGTT	1 cut(s)	129
AclWI	GGATC	1 cut(s)	500
AcoI	YGGCCR	1 cut(s)	246
AcsI	RAATTY	1 cut(s)	989
AfaI	GTAC	3 cut(s)	769, 945, 1136
AfeI	AGCGCT	2 cut(s)	12, 304
AfiI	CCNNNNNNNGG	2 cut(s)	569, 1105
AgsI	TTSAA	8 cut(s)	127, 134, 323, 519, 674, 784, 995, 1271
AjnI	CCWGG	1 cut(s)	873
AjuI	GAANNNNNNNTTGG	2 cut(s)	987, 1019
AluBI	AGCT	7 cut(s)	166, 267, 314, 728, 761, 986, 1089
AluI	AGCT	7 cut(s)	166, 267, 314, 728, 761, 986, 1089
Alw21I	GWGCWC	1 cut(s)	168
Alw26I	GTCTC	2 cut(s)	649, 909
AlwI	GGATC	1 cut(s)	500
Aor51HI	AGCGCT	2 cut(s)	12, 304
AoxI	GGCC	3 cut(s)	246, 370, 971
ApeKI	GCWGC	1 cut(s)	197
ApoI	RAATTY	1 cut(s)	989
AspLEI	GCGC	5 cut(s)	13, 29, 145, 305, 735
AspS9I	GGNCC	5 cut(s)	154, 352, 404, 937, 1096
AsuHPI	GGTGA	2 cut(s)	584, 689
AvaII	GGWCC	5 cut(s)	154, 352, 404, 937, 1096
AxyI	CCTNAGG	1 cut(s)	361
BanI	GGYRCC	1 cut(s)	614
BanII	GRGCYC	2 cut(s)	168, 1079
BauI	CACGAG	1 cut(s)	660
Bbv12I	GWGCWC	1 cut(s)	168
BbvI	GCAGC	1 cut(s)	184
BccI	CCATC	1 cut(s)	1048
BceAI	ACGGC	1 cut(s)	199
BciT130I	CCWGG	1 cut(s)	875
BclI	TGATCA	1 cut(s)	565
BcoDI	GTCTC	2 cut(s)	649, 909
BfaI	CTAG	2 cut(s)	296, 431
BfmI	CTRYAG	1 cut(s)	888
BfoI	RGCGCY	3 cut(s)	14, 146, 306
BglII	AGATCT	1 cut(s)	608
BisI	GCNGC	3 cut(s)	198, 246, 370
BlsI	GCNGC	3 cut(s)	199, 247, 371
BmcAI	AGTACT	1 cut(s)	1136
Bme1390I	CCNGG	1 cut(s)	875
Bme18I	GGWCC	5 cut(s)	154, 352, 404, 937, 1096
BmgT120I	GGNCC	5 cut(s)	154, 352, 404, 937, 1096
BmiI	GGNNCC	4 cut(s)	155, 616, 938, 1097
BmrFI	CCNGG	1 cut(s)	875
BmrI	ACTGGG	1 cut(s)	327
BmsI	GCATC	4 cut(s)	478, 678, 761, 1189
BmuI	ACTGGG	1 cut(s)	327
BpmI	CTGGAG	2 cut(s)	260, 858
BpuEI	CTTGAG	2 cut(s)	605, 1159
BsaXI	ACNNNNNCTCC	6 cut(s)	303, 333, 522, 552, 999, 1029
Bsc4I	CCNNNNNNNGG	2 cut(s)	569, 1105
Bse1I	ACTGG	4 cut(s)	243, 333, 1210, 1241
Bse21I	CCTNAGG	1 cut(s)	361
Bse3DI	GCAATG	2 cut(s)	1127, 1220
BseBI	CCWGG	1 cut(s)	875
BseGI	GGATG	4 cut(s)	693, 770, 1063, 1261
BseLI	CCNNNNNNNGG	2 cut(s)	569, 1105
BseMI	GCAATG	2 cut(s)	1127, 1220
BseMII	CTCAG	3 cut(s)	5, 153, 879
BseNI	ACTGG	4 cut(s)	243, 333, 1210, 1241
BseRI	GAGGAG	1 cut(s)	72
BseXI	GCAGC	1 cut(s)	184
BsgI	GTGCAG	2 cut(s)	476, 625
Bsh1285I	CGRYCG	1 cut(s)	226
BshFI	GGCC	3 cut(s)	248, 372, 973
BshNI	GGYRCC	1 cut(s)	614
BsiEI	CGRYCG	1 cut(s)	226
BsiHKAI	GWGCWC	1 cut(s)	168
BslFI	GGGAC	2 cut(s)	1109, 1122
BslI	CCNNNNNNNGG	2 cut(s)	569, 1105
BsmAI	GTCTC	2 cut(s)	649, 909
BsmBI	CGTCTC	1 cut(s)	909
BsmFI	GGGAC	2 cut(s)	1109, 1122
BsnI	GGCC	3 cut(s)	248, 372, 973
Bsp1286I	GDGCHC	2 cut(s)	168, 1079
Bsp143I	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
BspACI	CCGC	7 cut(s)	121, 157, 195, 226, 245, 309, 369
BspANI	GGCC	3 cut(s)	248, 372, 973
BspCNI	CTCAG	3 cut(s)	6, 154, 878
BspLI	GGNNCC	4 cut(s)	155, 616, 938, 1097
BspPI	GGATC	1 cut(s)	500
BspT107I	GGYRCC	1 cut(s)	614
BsrBI	CCGCTC	1 cut(s)	159
BsrDI	GCAATG	2 cut(s)	1127, 1220
BsrI	ACTGG	4 cut(s)	243, 333, 1210, 1241
BssMI	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
BssSI	CACGAG	1 cut(s)	660
Bst2BI	CACGAG	1 cut(s)	660
Bst2UI	CCWGG	1 cut(s)	875
Bst6I	CTCTTC	2 cut(s)	221, 1111
BstAPI	GCANNNNNTGC	2 cut(s)	440, 497
BstC8I	GCNNGC	5 cut(s)	31, 307, 560, 708, 712
BstDEI	CTNAG	4 cut(s)	14, 162, 361, 865
BstF5I	GGATG	4 cut(s)	693, 770, 1063, 1261
BstH2I	RGCGCY	3 cut(s)	14, 146, 306
BstHHI	GCGC	5 cut(s)	13, 29, 145, 305, 735
BstKTI	GATC	6 cut(s)	508, 568, 598, 611, 625, 684
BstMAI	GTCTC	2 cut(s)	649, 909
BstMBI	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
BstMCI	CGRYCG	1 cut(s)	226
BstMWI	GCNNNNNNNGC	7 cut(s)	242, 273, 311, 440, 497, 720, 758
BstNI	CCWGG	1 cut(s)	875
BstNSI	RCATGY	1 cut(s)	500
BstSCI	CCNGG	1 cut(s)	873
BstSFI	CTRYAG	1 cut(s)	888
BstV1I	GCAGC	1 cut(s)	184
BstX2I	RGATCY	1 cut(s)	608
BstYI	RGATCY	1 cut(s)	608
Bsu36I	CCTNAGG	1 cut(s)	361
BsuRI	GGCC	3 cut(s)	248, 372, 973
BtsCI	GGATG	4 cut(s)	693, 770, 1063, 1261
BtsIMutI	CAGTG	2 cut(s)	236, 340
Cac8I	GCNNGC	5 cut(s)	31, 307, 560, 708, 712
CfoI	GCGC	5 cut(s)	13, 29, 145, 305, 735
Cfr13I	GGNCC	5 cut(s)	154, 352, 404, 937, 1096
Csp6I	GTAC	3 cut(s)	768, 944, 1135
CviAII	CATG	5 cut(s)	34, 250, 440, 497, 569
CviQI	GTAC	3 cut(s)	768, 944, 1135
DdeI	CTNAG	4 cut(s)	14, 162, 361, 865
DpnI	GATC	6 cut(s)	507, 567, 597, 610, 624, 683
DpnII	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
EaeI	YGGCCR	1 cut(s)	246
Eam1104I	CTCTTC	2 cut(s)	221, 1111
EarI	CTCTTC	2 cut(s)	221, 1111
EciI	GGCGGA	1 cut(s)	324
Ecl136II	GAGCTC	1 cut(s)	166
Eco24I	GRGCYC	2 cut(s)	168, 1079
Eco32I	GATATC	1 cut(s)	259
Eco47I	GGWCC	5 cut(s)	154, 352, 404, 937, 1096
Eco47III	AGCGCT	2 cut(s)	12, 304
Eco53kI	GAGCTC	1 cut(s)	166
Eco81I	CCTNAGG	1 cut(s)	361
EcoICRI	GAGCTC	1 cut(s)	166
EcoRII	CCWGG	1 cut(s)	873
EcoRV	GATATC	1 cut(s)	259
EcoT22I	ATGCAT	1 cut(s)	754
EcoT38I	GRGCYC	2 cut(s)	168, 1079
Esp3I	CGTCTC	1 cut(s)	909
FaeI	CATG	5 cut(s)	37, 253, 443, 500, 572
FalI	AAGNNNNNCTT	2 cut(s)	699, 731
FaqI	GGGAC	2 cut(s)	1109, 1122
FatI	CATG	5 cut(s)	33, 249, 439, 496, 568
FbaI	TGATCA	1 cut(s)	565
Fnu4HI	GCNGC	3 cut(s)	198, 246, 370
FokI	GGATG	4 cut(s)	700, 777, 1070, 1268
FriOI	GRGCYC	2 cut(s)	168, 1079
Fsp4HI	GCNGC	3 cut(s)	198, 246, 370
FspBI	CTAG	2 cut(s)	296, 431
FspI	TGCGCA	1 cut(s)	734
GlaI	GCGC	5 cut(s)	12, 28, 144, 304, 734
GluI	GCNGC	3 cut(s)	198, 246, 370
GsuI	CTGGAG	2 cut(s)	260, 858
HaeII	RGCGCY	3 cut(s)	14, 146, 306
HaeIII	GGCC	3 cut(s)	248, 372, 973
HhaI	GCGC	5 cut(s)	13, 29, 145, 305, 735
Hin1II	CATG	5 cut(s)	37, 253, 443, 500, 572
Hin6I	GCGC	5 cut(s)	11, 27, 143, 303, 733
HinP1I	GCGC	5 cut(s)	11, 27, 143, 303, 733
HincII	GTYRAC	1 cut(s)	181
HindII	GTYRAC	1 cut(s)	181
HindIII	AAGCTT	1 cut(s)	726
HinfI	GANTC	4 cut(s)	203, 638, 784, 820
HphI	GGTGA	2 cut(s)	584, 689
Hpy166II	GTNNAC	3 cut(s)	51, 181, 944
Hpy188I	TCNGA	3 cut(s)	163, 208, 549
Hpy188III	TCNNGA	7 cut(s)	685, 744, 781, 824, 837, 1160, 1253
Hpy8I	GTNNAC	3 cut(s)	51, 181, 944
HpyAV	CCTTC	2 cut(s)	668, 1066
HpyCH4IV	ACGT	2 cut(s)	129, 883
HpyF10VI	GCNNNNNNNGC	7 cut(s)	242, 273, 311, 440, 497, 720, 758
HpyF3I	CTNAG	4 cut(s)	14, 162, 361, 865
HpySE526I	ACGT	2 cut(s)	129, 883
Hsp92II	CATG	5 cut(s)	37, 253, 443, 500, 572
HspAI	GCGC	5 cut(s)	11, 27, 143, 303, 733
Ksp22I	TGATCA	1 cut(s)	565
Kzo9I	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
LmnI	GCTCC	3 cut(s)	171, 311, 1190
Lsp1109I	GCAGC	1 cut(s)	184
LweI	GCATC	4 cut(s)	478, 678, 761, 1189
MaeI	CTAG	2 cut(s)	296, 431
MaeII	ACGT	2 cut(s)	129, 883
MaeIII	GTNAC	3 cut(s)	286, 829, 1243
MalI	GATC	6 cut(s)	507, 567, 597, 610, 624, 683
MbiI	CCGCTC	1 cut(s)	159
MboI	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
MboII	GAAGA	7 cut(s)	208, 1081, 1128, 1148, 1186, 1271, 1283
MfeI	CAATTG	2 cut(s)	444, 1022
MflI	RGATCY	1 cut(s)	608
MhlI	GDGCHC	2 cut(s)	168, 1079
MluCI	AATT	8 cut(s)	387, 444, 669, 843, 924, 989, 996, 1022
MlyI	GAGTC	1 cut(s)	197
MmeI	TCCRAC	1 cut(s)	231
Mph1103I	ATGCAT	1 cut(s)	754
MseI	TTAA	1 cut(s)	851
MslI	CAYNNNNRTG	1 cut(s)	1254
MspA1I	CMGCKG	1 cut(s)	197
MspR9I	CCNGG	1 cut(s)	875
MunI	CAATTG	2 cut(s)	444, 1022
MvaI	CCWGG	1 cut(s)	875
MwoI	GCNNNNNNNGC	7 cut(s)	242, 273, 311, 440, 497, 720, 758
NdeII	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
NlaIII	CATG	5 cut(s)	37, 253, 443, 500, 572
NlaIV	GGNNCC	4 cut(s)	155, 616, 938, 1097
NmeAIII	GCCGAG	1 cut(s)	626
NmuCI	GTSAC	1 cut(s)	829
NsbI	TGCGCA	1 cut(s)	734
NsiI	ATGCAT	1 cut(s)	754
NspI	RCATGY	1 cut(s)	500
PfeI	GAWTC	3 cut(s)	638, 784, 820
PflMI	CCANNNNNTGG	1 cut(s)	1105
PkrI	GCNGC	3 cut(s)	199, 247, 371
PleI	GAGTC	1 cut(s)	197
PpsI	GAGTC	1 cut(s)	197
Psp124BI	GAGCTC	1 cut(s)	168
Psp1406I	AACGTT	1 cut(s)	129
Psp6I	CCWGG	1 cut(s)	873
PspGI	CCWGG	1 cut(s)	873
PspN4I	GGNNCC	4 cut(s)	155, 616, 938, 1097
PspPI	GGNCC	5 cut(s)	154, 352, 404, 937, 1096
PsuI	RGATCY	1 cut(s)	608
RsaI	GTAC	3 cut(s)	769, 945, 1136
RsaNI	GTAC	3 cut(s)	768, 944, 1135
RseI	CAYNNNNRTG	1 cut(s)	1254
SacI	GAGCTC	1 cut(s)	168
SaqAI	TTAA	1 cut(s)	851
SatI	GCNGC	3 cut(s)	198, 246, 370
Sau3AI	GATC	6 cut(s)	505, 565, 595, 608, 622, 681
Sau96I	GGNCC	5 cut(s)	154, 352, 404, 937, 1096
ScaI	AGTACT	1 cut(s)	1136
SchI	GAGTC	1 cut(s)	197
ScrFI	CCNGG	1 cut(s)	875
SduI	GDGCHC	2 cut(s)	168, 1079
SfaNI	GCATC	4 cut(s)	478, 678, 761, 1189
SfcI	CTRYAG	1 cut(s)	888
SinI	GGWCC	5 cut(s)	154, 352, 404, 937, 1096
SmiMI	CAYNNNNRTG	1 cut(s)	1254
SmlI	CTYRAG	2 cut(s)	584, 1138
SmoI	CTYRAG	2 cut(s)	584, 1138
Sse9I	AATT	8 cut(s)	387, 444, 669, 843, 924, 989, 996, 1022
SsiI	CCGC	7 cut(s)	121, 157, 195, 226, 245, 309, 369
SspMI	CTAG	2 cut(s)	296, 431
SstI	GAGCTC	1 cut(s)	168
StyD4I	CCNGG	1 cut(s)	873
TaiI	ACGT	2 cut(s)	132, 886
TaqI	TCGA	3 cut(s)	222, 636, 968
TasI	AATT	8 cut(s)	387, 444, 669, 843, 924, 989, 996, 1022
TatI	WGTACW	2 cut(s)	767, 1134
TauI	GCSGC	2 cut(s)	248, 372
TfiI	GAWTC	3 cut(s)	638, 784, 820
Tru1I	TTAA	1 cut(s)	851
Tru9I	TTAA	1 cut(s)	851
TscAI	CASTG	2 cut(s)	243, 340
TseFI	GTSAC	1 cut(s)	829
TseI	GCWGC	1 cut(s)	197
Tsp45I	GTSAC	1 cut(s)	829
TspDTI	ATGAA	2 cut(s)	266, 1272
TspGWI	ACGGA	1 cut(s)	929
TspRI	CASTG	2 cut(s)	243, 340
Van91I	CCANNNNNTGG	1 cut(s)	1105
VpaK11BI	GGWCC	5 cut(s)	154, 352, 404, 937, 1096
XapI	RAATTY	1 cut(s)	989
XceI	RCATGY	1 cut(s)	500
XcmI	CCANNNNNNNNNTGG	1 cut(s)	566
XspI	CTAG	2 cut(s)	296, 431
ZrmI	AGTACT	1 cut(s)	1136
Zsp2I	ATGCAT	1 cut(s)	754

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.